RchiOBHm_Chr2g0134781

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000353
Physical Location & Seq
Reverse (-)
51896523 .. 51900645
4123 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ50578

Sequence Viewer

Length: 2283 bp
ATGAGTTCACGCGAGGAAGAAATGGGAATCAGAGGTAAAGCACAGAAGAGAGAAGAGGCGGTGGCGGTGGCCATCGACAAGGACAAAGGAAGTCAATATGCTATCAAATGGACTGTTGATCATCTTCTCAGCAGAGGCCAAGCTCTCACATTGCTTCATGTTAAACACACTACAAATGCAGTTCCCAACCAATCCAGAGGCACGACTTTTATGTCTGAGGATGTTGCAAAGGTGTACAAAAAGCAACTTGATGTTCAAGCAAAAGAGTTGTTTCTTCCATTCCGTTGCTTTTGCACCAAAAAGGCTATAAAATGCAATGAAGTCATAGTTGAGGATACAGATATAACAAGAGGAATCATCAACTATGTCAATGCCAATGGGATTGAAATTTTGGTACTTGGTTCTCCATCAAAAAGCAGCTTATTCAGATTCATATCTACAGACGTTCCAACCACCGTGGCGAAAAGGGCACCAGACTGTTGCACTGTTTATGTTATTGGGAAAGGAAAGATCTCATATGTGCGATCCGCTACCAGCTCATTGCCCCCAAAAGCTCCATCGAGTAATCAAATACAGCACCAAGCTAGCAACGTTTCAGATTCAAGTGATACACGAAGCACACCCAGCCAGAACTTTAGAGGACCCGAGCAGAAACAGCATCCGGCCCATACCCAGCAAAATGACAATGCAATCCTGTCACCATTCTCAAGAGGAAAAGCTTCAACGAACAAAACACATGAGCTATCACCCGAGAGTGATATATCGTTTGTGAGCAGTAACAGAAGGTCAAGCACTGATAACAGAAGCTCTACATCATCTTACTCTGGAACCAAGTTCATTGACATGGGCTCTGCCCACAATGACTTCACATCATGCTCAATGGATAGTGGCAAATCGTGGTCATCACAGAACATGGATGGAGTGGAATCAGAGATGAGGAGGCTTATGCTAGAGCTCAAGCAAACAAGGGACATGTACAATACAGCCTGCAAGGAGGCACTCGTAGCACAAAATATGGCAACAGAAATAAACCAATGGAAAAGGGCAGAAGAAATAAGATTAGAAGAGGCAAAAGTAGCTGAGGAAGTTGCAATGGCAGCTGCAGAGAAGGAGAAAGCCAAATGTATGGCAGCCATCGAAGCAGCACAAGCAGCTCAAAGGGTTGCAGAATTCGAGGCACTGAAAAGGAAGACTGCAGAAATGAAAGCCCTTAAAGAAGCGGAAGACATGAAGAAGGCACAAGAAGGAATGCCATTTGATGTCAGGTACAGGAAGTACACACTTGAAGAGATTGAAGCAGCAACACACAGCTTTTCAGGTACTCTCAAGATTGGGGAAGGAGGCTATGGGCCTGTGTATAAAGGTGAACTAGACCATACATCTGTGGCCATAAAAGTTCTACGTCCAGATGCAGCTCAAGGGCATTCACAGTTCAAGCGAGAGGTGGATGTTTTGAGCTGCATACGACATCCAAACATGGTCCTCCTTCTTGGAGCATGTCCCGAGTACGGTTGCCTTGTCTACGAGTACATGGCTAATGGTAGCTTAGAAGATCGTCTCTTCCGGAAAGGTAATACCCCAGCTATTCCTTGGCAGCTAAGGTTCCGAATTGCAGCAGAAATTGCCACAGGGCTTTTGTTCCTCCATCAGAGCAAGCCAGAACCCCTTGTACACCGTGACCTTAAACCCGGAAATATTTTGCTGGACCATAACTATGTCAGCAAGATTAGTGATGTAGGTTTGGCTAGACTTGTCCCTCCATCAGTAGCCGACTCTGTCACTCAGTATTACATGACATCAACAGCAGGAACATTCTGCTATATCGATCCAGAGTATCAGCAAACCGGCATGCTTGGAACAAAATCTGACATCTACTCACTTGGAGTCATGCTTCTGCAAATCATAACAGCCACGCCACCAATGGGTTTGACACATCATGTTGAGAGGGCTATTGAGAAAGGGACCTTTGCTGACATGCTTGACCCTGCTGTTAAAGACTGGCCTGTTGAGGAGGCCTTGAACTTTGCCAGGCTAGCTTTACAGTGTGCTGAAATGAGGCGAAAAGACAGACCGGATCTTGGGAAGTTTGTGCTACCTGAGCTTAACAGGTTGAGAGCGCTTGGCCAAGATAGCATGAATGCTAAGATGTTTGGTGGCGGTGAAGTATTCTCTCCAAGAAAAGGCCGATTTTCCACCGGACAATTACAGGATGTGATGAGTGACCCCGATCTACGACAATTTGGTTATGATAGCTCAAGGAGTCAATCAAGCACATCATCTTAA

Protein Analysis

760

Amino Acids

84.28

Weight (kDa)

8.24

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Usp PF00582 21 - 158 1.1e-12 Universal stress protein family
Pkinase PF00069 443 - 691 7e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 443 - 694 1e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 211
AccB1I GGYRCC 1 cut(s) 469
AccI GTMKAC 1 cut(s) 1521
AccII CGCG 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 1563
AciI CCGC 5 cut(s) 59, 65, 528, 1220, 2157
AclI AACGTT 1 cut(s) 591
AclWI GGATC 3 cut(s) 519, 1820, 2082
AcoI YGGCCR 3 cut(s) 69, 1386, 2122
AcsI RAATTY 2 cut(s) 387, 1169
AfaI GTAC 9 cut(s) 236, 396, 977, 1268, 1277, 1321, 1508, 1529, 1671
AfeI AGCGCT 1 cut(s) 2118
AfiI CCNNNNNNNGG 4 cut(s) 1508, 1922, 2078, 2180
AflIII ACRYGT 1 cut(s) 972
AgsI TTSAA 8 cut(s) 257, 386, 603, 723, 1286, 1295, 1435, 2020
AjnI CCWGG 1 cut(s) 2027
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 1 cut(s) 1562
AlwI GGATC 3 cut(s) 519, 1820, 2082
Ama87I CYCGRG 3 cut(s) 644, 749, 1502
Aor13HI TCCGGA 1 cut(s) 1563
Aor51HI AGCGCT 1 cut(s) 2118
AoxI GGCC 9 cut(s) 69, 136, 663, 1349, 1386, 2000, 2013, 2122, 2182
ApoI RAATTY 2 cut(s) 387, 1169
Asp700I GAANNNNTTC 1 cut(s) 718
AspLEI GCGC 1 cut(s) 2119
AspS9I GGNCC 6 cut(s) 641, 664, 1349, 1480, 1705, 1962
AsuC2I CCSGG 1 cut(s) 1689
AsuHPI GGTGA 4 cut(s) 690, 738, 1376, 2171
AsuNHI GCTAGC 2 cut(s) 584, 2032
AvaI CYCGRG 3 cut(s) 644, 749, 1502
AvaII GGWCC 4 cut(s) 641, 1480, 1705, 1962
BaeGI GKGCMC 1 cut(s) 472
BalI TGGCCA 3 cut(s) 71, 1388, 2124
BanI GGYRCC 1 cut(s) 469
BanII GRGCYC 2 cut(s) 851, 957
BbsI GAAGAC 2 cut(s) 1196, 1230
Bbv12I GWGCWC 1 cut(s) 957
BbvCI CCTCAGC 1 cut(s) 1080
BccI CCATC 7 cut(s) 80, 415, 565, 911, 1142, 1653, 1768
BciT130I CCWGG 1 cut(s) 2029
BciVI GTATCC 1 cut(s) 328
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 1 cut(s) 1689
BcoDI GTCTC 1 cut(s) 1562
BfaI CTAG 5 cut(s) 585, 950, 1370, 1746, 2033
BfmI CTRYAG 3 cut(s) 438, 1101, 1194
BfoI RGCGCY 1 cut(s) 2120
BfuI GTATCC 1 cut(s) 328
BglII AGATCT 1 cut(s) 510
Bme1390I CCNGG 2 cut(s) 1689, 2029
Bme18I GGWCC 4 cut(s) 641, 1480, 1705, 1962
BmeT110I CYCGRG 3 cut(s) 644, 749, 1502
BmgT120I GGNCC 6 cut(s) 641, 664, 1349, 1480, 1705, 1962
BmiI GGNNCC 5 cut(s) 471, 643, 829, 1604, 1963
BmrFI CCNGG 2 cut(s) 1689, 2029
BmsI GCATC 2 cut(s) 667, 1399
BmtI GCTAGC 2 cut(s) 588, 2036
BpiI GAAGAC 2 cut(s) 1196, 1230
Bpu10I CCTNAGC 3 cut(s) 1080, 1598, 2097
BpuEI CTTGAG 5 cut(s) 691, 941, 1310, 1401, 2239
BpuMI CCSGG 1 cut(s) 1689
Bsa29I ATCGAT 1 cut(s) 1824
BsaBI GATNNNNATC 1 cut(s) 433
BsaJI CCNNGG 2 cut(s) 456, 1589
BsaWI WCCGGW 3 cut(s) 1563, 2071, 2195
Bsc4I CCNNNNNNNGG 4 cut(s) 1508, 1922, 2078, 2180
Bse118I RCCGGY 1 cut(s) 1844
Bse1I ACTGG 1 cut(s) 2003
Bse3DI GCAATG 4 cut(s) 149, 322, 539, 1098
Bse8I GATNNNNATC 1 cut(s) 433
BseAI TCCGGA 1 cut(s) 1563
BseBI CCWGG 1 cut(s) 2029
BseCI ATCGAT 1 cut(s) 1824
BseDI CCNNGG 2 cut(s) 456, 1589
BseGI GGATG 6 cut(s) 226, 658, 922, 1453, 1468, 2215
BseJI GATNNNNATC 1 cut(s) 433
BseLI CCNNNNNNNGG 4 cut(s) 1508, 1922, 2078, 2180
BseMI GCAATG 4 cut(s) 149, 322, 539, 1098
BseMII CTCAG 5 cut(s) 142, 207, 1071, 1796, 2088
BseNI ACTGG 1 cut(s) 2003
BseRI GAGGAG 2 cut(s) 952, 2024
BseSI GKGCMC 1 cut(s) 472
BseYI CCCAGC 3 cut(s) 623, 672, 1579
Bsh1236I CGCG 1 cut(s) 12
BshFI GGCC 9 cut(s) 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184
BshNI GGYRCC 1 cut(s) 469
BshVI ATCGAT 1 cut(s) 1824
BsiHKAI GWGCWC 1 cut(s) 957
BsiHKCI CYCGRG 3 cut(s) 644, 749, 1502
BsiSI CCGG 6 cut(s) 662, 1564, 1689, 1845, 2072, 2196
BslFI GGGAC 4 cut(s) 983, 1485, 1739, 1975
BslI CCNNNNNNNGG 4 cut(s) 1508, 1922, 2078, 2180
BsmAI GTCTC 1 cut(s) 1562
BsmBI CGTCTC 1 cut(s) 1562
BsmFI GGGAC 4 cut(s) 983, 1485, 1739, 1975
BsmI GAATGC 3 cut(s) 1254, 1423, 2143
BsnI GGCC 9 cut(s) 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184
BsoBI CYCGRG 3 cut(s) 644, 749, 1502
Bsp1286I GDGCHC 3 cut(s) 472, 851, 957
Bsp13I TCCGGA 1 cut(s) 1563
Bsp1407I TGTACA 3 cut(s) 234, 975, 1669
Bsp143I GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
BspACI CCGC 5 cut(s) 59, 65, 528, 1220, 2157
BspANI GGCC 9 cut(s) 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184
BspCNI CTCAG 5 cut(s) 141, 208, 1072, 1795, 2089
BspDI ATCGAT 1 cut(s) 1824
BspEI TCCGGA 1 cut(s) 1563
BspFNI CGCG 1 cut(s) 12
BspLI GGNNCC 5 cut(s) 471, 643, 829, 1604, 1963
BspMAI CTGCAG 2 cut(s) 1105, 1198
BspOI GCTAGC 2 cut(s) 588, 2036
BspPI GGATC 3 cut(s) 519, 1820, 2082
BspT107I GGYRCC 1 cut(s) 469
BsrDI GCAATG 4 cut(s) 149, 322, 539, 1098
BsrFI RCCGGY 1 cut(s) 1844
BsrGI TGTACA 3 cut(s) 234, 975, 1669
BsrI ACTGG 1 cut(s) 2003
BssAI RCCGGY 1 cut(s) 1844
BssECI CCNNGG 2 cut(s) 456, 1589
BssMI GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
BssT1I CCWWGG 1 cut(s) 1589
Bst2UI CCWGG 1 cut(s) 2029
Bst4CI ACNGT 8 cut(s) 115, 457, 479, 487, 1431, 1511, 1676, 2043
Bst6I CTCTTC 5 cut(s) 41, 48, 1059, 1281, 1565
BstAPI GCANNNNNTGC 1 cut(s) 1622
BstAUI TGTACA 3 cut(s) 234, 975, 1669
BstC8I GCNNGC 5 cut(s) 586, 988, 1655, 1850, 2034
BstDEI CTNAG 8 cut(s) 128, 216, 1080, 1546, 1598, 1782, 2097, 2142
BstDSI CCRYGG 1 cut(s) 456
BstF5I GGATG 6 cut(s) 226, 658, 922, 1453, 1468, 2215
BstFNI CGCG 1 cut(s) 12
BstH2I RGCGCY 1 cut(s) 2120
BstHHI GCGC 1 cut(s) 2119
BstKTI GATC 7 cut(s) 121, 513, 527, 1555, 1828, 2077, 2230
BstMAI GTCTC 1 cut(s) 1562
BstMBI GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
BstNI CCWGG 1 cut(s) 2029
BstNSI RCATGY 4 cut(s) 976, 1500, 1852, 1978
BstSCI CCNGG 2 cut(s) 1687, 2027
BstSFI CTRYAG 3 cut(s) 438, 1101, 1194
BstSLI GKGCMC 1 cut(s) 472
BstUI CGCG 1 cut(s) 12
BstV2I GAAGAC 2 cut(s) 1196, 1230
BstX2I RGATCY 2 cut(s) 510, 2074
BstXI CCANNNNNNTGG 1 cut(s) 1126
BstYI RGATCY 2 cut(s) 510, 2074
Bsu15I ATCGAT 1 cut(s) 1824
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 9 cut(s) 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184
BsuTUI ATCGAT 1 cut(s) 1824
BtgI CCRYGG 1 cut(s) 456
BtsCI GGATG 6 cut(s) 226, 658, 922, 1453, 1468, 2215
BtsIMutI CAGTG 4 cut(s) 483, 792, 1178, 2048
Cac8I GCNNGC 5 cut(s) 586, 988, 1655, 1850, 2034
CfoI GCGC 1 cut(s) 2119
Cfr10I RCCGGY 1 cut(s) 1844
Cfr13I GGNCC 6 cut(s) 641, 664, 1349, 1480, 1705, 1962
ClaI ATCGAT 1 cut(s) 1824
Csp6I GTAC 9 cut(s) 235, 395, 976, 1267, 1276, 1320, 1507, 1528, 1670
CspCI CAANNNNNGTGG 2 cut(s) 438, 473
CviQI GTAC 9 cut(s) 235, 395, 976, 1267, 1276, 1320, 1507, 1528, 1670
DdeI CTNAG 8 cut(s) 128, 216, 1080, 1546, 1598, 1782, 2097, 2142
DpnI GATC 7 cut(s) 120, 512, 526, 1554, 1827, 2076, 2229
DpnII GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
DrdI GACNNNNNNGTC 1 cut(s) 211
DseDI GACNNNNNNGTC 1 cut(s) 211
EaeI YGGCCR 3 cut(s) 69, 1386, 2122
Eam1104I CTCTTC 5 cut(s) 41, 48, 1059, 1281, 1565
EarI CTCTTC 5 cut(s) 41, 48, 1059, 1281, 1565
Ecl136II GAGCTC 1 cut(s) 955
Eco130I CCWWGG 1 cut(s) 1589
Eco147I AGGCCT 1 cut(s) 2015
Eco24I GRGCYC 2 cut(s) 851, 957
Eco47I GGWCC 4 cut(s) 641, 1480, 1705, 1962
Eco47III AGCGCT 1 cut(s) 2118
Eco53kI GAGCTC 1 cut(s) 955
Eco88I CYCGRG 3 cut(s) 644, 749, 1502
EcoICRI GAGCTC 1 cut(s) 955
EcoO109I RGGNCCY 2 cut(s) 641, 1962
EcoRI GAATTC 1 cut(s) 1169
EcoRII CCWGG 1 cut(s) 2027
EcoT14I CCWWGG 1 cut(s) 1589
EcoT38I GRGCYC 2 cut(s) 851, 957
ErhI CCWWGG 1 cut(s) 1589
Esp3I CGTCTC 1 cut(s) 1562
FaqI GGGAC 4 cut(s) 983, 1485, 1739, 1975
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 118
FblI GTMKAC 1 cut(s) 1521
FokI GGATG 6 cut(s) 233, 645, 929, 1455, 1460, 2222
FriOI GRGCYC 2 cut(s) 851, 957
FspBI CTAG 5 cut(s) 585, 950, 1370, 1746, 2033
GlaI GCGC 1 cut(s) 2118
GsaI CCCAGC 3 cut(s) 627, 676, 1583
HaeII RGCGCY 1 cut(s) 2120
HaeIII GGCC 9 cut(s) 71, 138, 665, 1351, 1388, 2002, 2015, 2124, 2184
HapII CCGG 6 cut(s) 662, 1564, 1689, 1845, 2072, 2196
HhaI GCGC 1 cut(s) 2119
Hin6I GCGC 1 cut(s) 2117
HinP1I GCGC 1 cut(s) 2117
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 8 cut(s) 27, 354, 429, 599, 926, 1772, 1884, 2260
HpaII CCGG 6 cut(s) 662, 1564, 1689, 1845, 2072, 2196
HphI GGTGA 4 cut(s) 690, 738, 1376, 2171
Hpy166II GTNNAC 6 cut(s) 8, 235, 1278, 1367, 1522, 1672
Hpy188I TCNGA 8 cut(s) 32, 217, 428, 598, 931, 1607, 1650, 1867
Hpy188III TCNNGA 8 cut(s) 195, 708, 825, 1327, 1406, 1502, 1564, 1829
Hpy8I GTNNAC 6 cut(s) 8, 235, 1278, 1367, 1522, 1672
HpyAV CCTTC 6 cut(s) 777, 1102, 1228, 1238, 1331, 1496
HpyCH4III ACNGT 8 cut(s) 115, 457, 479, 487, 1431, 1511, 1676, 2043
HpyCH4IV ACGT 3 cut(s) 444, 591, 1402
HpyF3I CTNAG 8 cut(s) 128, 216, 1080, 1546, 1598, 1782, 2097, 2142
HpySE526I ACGT 3 cut(s) 444, 591, 1402
HspAI GCGC 1 cut(s) 2117
Kpn2I TCCGGA 1 cut(s) 1563
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
LmnI GCTCC 2 cut(s) 559, 1493
LweI GCATC 2 cut(s) 667, 1399
MaeI CTAG 5 cut(s) 585, 950, 1370, 1746, 2033
MaeII ACGT 3 cut(s) 444, 591, 1402
MaeIII GTNAC 5 cut(s) 696, 776, 1676, 1777, 2219
MalI GATC 7 cut(s) 120, 512, 526, 1554, 1827, 2076, 2229
MboI GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
MflI RGATCY 2 cut(s) 510, 2074
MhlI GDGCHC 3 cut(s) 472, 851, 957
MlsI TGGCCA 3 cut(s) 71, 1388, 2124
MluCI AATT 6 cut(s) 387, 1169, 1608, 1620, 2201, 2237
MluNI TGGCCA 3 cut(s) 71, 1388, 2124
MlyI GAGTC 3 cut(s) 1766, 1893, 2269
MmeI TCCRAC 1 cut(s) 473
Mox20I TGGCCA 3 cut(s) 71, 1388, 2124
MroI TCCGGA 1 cut(s) 1563
MroXI GAANNNNTTC 1 cut(s) 718
MscI TGGCCA 3 cut(s) 71, 1388, 2124
MseI TTAA 6 cut(s) 162, 1212, 1683, 1992, 2103, 2281
MslI CAYNNNNRTG 2 cut(s) 842, 1713
Msp20I TGGCCA 3 cut(s) 71, 1388, 2124
MspA1I CMGCKG 1 cut(s) 1100
MspI CCGG 6 cut(s) 662, 1564, 1689, 1845, 2072, 2196
MspR9I CCNGG 2 cut(s) 1689, 2029
Mva1269I GAATGC 3 cut(s) 1254, 1423, 2143
MvaI CCWGG 1 cut(s) 2029
MvnI CGCG 1 cut(s) 12
NciI CCSGG 1 cut(s) 1689
NdeI CATATG 1 cut(s) 517
NdeII GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
NheI GCTAGC 2 cut(s) 584, 2032
NlaIV GGNNCC 5 cut(s) 471, 643, 829, 1604, 1963
NmuCI GTSAC 4 cut(s) 696, 1676, 1777, 2219
NspI RCATGY 4 cut(s) 976, 1500, 1852, 1978
PaeI GCATGC 1 cut(s) 1852
PceI AGGCCT 1 cut(s) 2015
PciI ACATGT 1 cut(s) 972
PctI GAATGC 3 cut(s) 1254, 1423, 2143
PdmI GAANNNNTTC 1 cut(s) 718
PfeI GAWTC 5 cut(s) 27, 354, 429, 599, 926
PflFI GACNNNGTC 1 cut(s) 1775
PleI GAGTC 3 cut(s) 1766, 1892, 2268
PpsI GAGTC 3 cut(s) 1766, 1892, 2268
PpuMI RGGWCCY 2 cut(s) 641, 1962
PscI ACATGT 1 cut(s) 972
Psp124BI GAGCTC 1 cut(s) 957
Psp1406I AACGTT 1 cut(s) 591
Psp5II RGGWCCY 2 cut(s) 641, 1962
Psp6I CCWGG 1 cut(s) 2027
PspFI CCCAGC 3 cut(s) 623, 672, 1579
PspGI CCWGG 1 cut(s) 2027
PspN4I GGNNCC 5 cut(s) 471, 643, 829, 1604, 1963
PspPI GGNCC 6 cut(s) 641, 664, 1349, 1480, 1705, 1962
PspPPI RGGWCCY 2 cut(s) 641, 1962
PsrI GAACNNNNNNTAC 2 cut(s) 1653, 1685
PstI CTGCAG 2 cut(s) 1105, 1198
PsuI RGATCY 2 cut(s) 510, 2074
PsyI GACNNNGTC 1 cut(s) 1775
PvuII CAGCTG 1 cut(s) 1100
RsaI GTAC 9 cut(s) 236, 396, 977, 1268, 1277, 1321, 1508, 1529, 1671
RsaNI GTAC 9 cut(s) 235, 395, 976, 1267, 1276, 1320, 1507, 1528, 1670
RseI CAYNNNNRTG 2 cut(s) 842, 1713
SacI GAGCTC 1 cut(s) 957
SaqAI TTAA 6 cut(s) 162, 1212, 1683, 1992, 2103, 2281
Sau3AI GATC 7 cut(s) 118, 510, 524, 1552, 1825, 2074, 2227
Sau96I GGNCC 6 cut(s) 641, 664, 1349, 1480, 1705, 1962
SchI GAGTC 3 cut(s) 1766, 1893, 2269
ScrFI CCNGG 2 cut(s) 1689, 2029
SduI GDGCHC 3 cut(s) 472, 851, 957
SfaNI GCATC 2 cut(s) 667, 1399
SfcI CTRYAG 3 cut(s) 438, 1101, 1194
SinI GGWCC 4 cut(s) 641, 1480, 1705, 1962
SmiMI CAYNNNNRTG 2 cut(s) 842, 1713
SmlI CTYRAG 5 cut(s) 706, 956, 1325, 1416, 2254
SmoI CTYRAG 5 cut(s) 706, 956, 1325, 1416, 2254
SphI GCATGC 1 cut(s) 1852
Sse9I AATT 6 cut(s) 387, 1169, 1608, 1620, 2201, 2237
SseBI AGGCCT 1 cut(s) 2015
SsiI CCGC 5 cut(s) 59, 65, 528, 1220, 2157
SspI AATATT 1 cut(s) 1696
SspMI CTAG 5 cut(s) 585, 950, 1370, 1746, 2033
SstI GAGCTC 1 cut(s) 957
StuI AGGCCT 1 cut(s) 2015
StyD4I CCNGG 2 cut(s) 1687, 2027
StyI CCWWGG 1 cut(s) 1589
TaaI ACNGT 8 cut(s) 115, 457, 479, 487, 1431, 1511, 1676, 2043
TaiI ACGT 3 cut(s) 447, 594, 1405
TaqI TCGA 5 cut(s) 75, 560, 1137, 1173, 1824
TasI AATT 6 cut(s) 387, 1169, 1608, 1620, 2201, 2237
TatI WGTACW 5 cut(s) 234, 975, 1275, 1527, 1669
TfiI GAWTC 5 cut(s) 27, 354, 429, 599, 926
Tru1I TTAA 6 cut(s) 162, 1212, 1683, 1992, 2103, 2281
Tru9I TTAA 6 cut(s) 162, 1212, 1683, 1992, 2103, 2281
TscAI CASTG 4 cut(s) 490, 799, 1185, 2048
TseFI GTSAC 4 cut(s) 696, 1676, 1777, 2219
Tsp45I GTSAC 4 cut(s) 696, 1676, 1777, 2219
TspDTI ATGAA 7 cut(s) 146, 333, 421, 826, 1217, 1244, 2150
TspGWI ACGGA 1 cut(s) 272
TspRI CASTG 4 cut(s) 490, 799, 1185, 2048
Tth111I GACNNNGTC 1 cut(s) 1775
VpaK11BI GGWCC 4 cut(s) 641, 1480, 1705, 1962
XapI RAATTY 2 cut(s) 387, 1169
XceI RCATGY 4 cut(s) 976, 1500, 1852, 1978
XcmI CCANNNNNNNNNTGG 2 cut(s) 1587, 1918
XmiI GTMKAC 1 cut(s) 1521
XmnI GAANNNNTTC 1 cut(s) 718
XspI CTAG 5 cut(s) 585, 950, 1370, 1746, 2033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.