pycom02g21950

U-box domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000353
Physical Location & Seq
Reverse (-)
20089005 .. 20094445
5441 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21950.8

Sequence Viewer

Length: 3396 bp
ATGAAAGGGGTGAAGGGAAAGTTGATGATGAAGTTGAAGTCAATCGGACCGATTGGAATTGGGTACCTGAAACCCCAAGATCGAGTTCTTCAGGTAAATGCATCGGATGGGTACGCGGATTCAAATCTCAACATTCAGACCCATAATTTGATTTCGGAAGAAACGGAGCAGAAGAAGAAGATCAAACAGTTAGAGAAGAATGTGACGGAGCAAGAGCCTGACGTTATCGACGTCACCGAGCTTATGAGAGACCTCGAAGATGAAGAAGAGTCAGGGCAGGATGGCGATGATATCGACGTCGACGACAAGGAAAACGTTAGGCCTAAAATGGTGGCAAAACACCCAGTAGAGTGCAGCAAAAGTTGGGTTAAAACGGAATCAAATTTGGAGGAAAACAGAGCTTCTGAAGCTTCAGAGAATCGTAGACAGACCCCTTTGTCGGAGATTGACATCTCATCTTTTCGGAGACCGGACTTAAACTCCGGTAGCCTCTTCGACCCGGACCTACTAGCAGCCTTTCAGCAAGCAGTGATGGAATATATGAGGTTGAGTGAAGAAGAGAAAAATGCAAGGAGAAGCGAAAAAGAAGATATTGAATCCAATTTTGTAGAAGCGCCAGATCCCGAGCCAGAGCTAGAGCCAGCACCACCTTCAAATGAAGACGCTCTGTTAGCCTTTGAAGAAAAGTGTCCGCCGGGTAGTTTGGATTCATCGGTAGTTCTCTACACTACAACCCTTCGGGGAATTCGGAAGACTTTTGATGATTGCAACAGCGTTCGATTTCTTTTGGAGAGCTTTCGGGTGGTGTTCTACGAGCGAGATGTGTCAATGCACATGGAATTCAGACAAGAGCTGTGGAAGATTTTGGATTGCAAAGCAGTGCCTCCAAAGCTTTTCATAAAGGGGAGATATATCGGGGGAGCGGAGGAGGTTTTGACGCTTCATGAACAAGGAAAGCTCAAGCCGCTCTTTGAAGGAGTCCCGTTGGATCGGTCCATTGGCCCCTGTGAAGCTTGTGATGGAGTTCGATTTGTGGTTTGCTTCAGATGCAGCGGGAGCTGCAAGCTCATCGCCGATGAGGGGCAGCCGGATAAGTGCCCCGAGTACAGATACCAAGTAGCTCGTAGCTTACAAGCCTGGTGGTACTTCTGTTTCCGGGGTCGATTGGAAGACAAATCATATAGAGAAATGGGAAGAGGGACAGTAGAATCGAGAGAGAAGGTGACACTTGCAATAGACAGAGACAAAGGAAGTCAACATGCTATAAAATGGGCTGTTGATAATCTTGTCACCGGAGGCCAACATGTCATTCTGCTCCATGTCAAACAAACAACGCCGGCGGTTTCTGCTGTATCCCAAGGGGAGGAAACTGAAAATGTGTACAAAAAACCAGTTGACCCAATAACCAAAGAGCTGTTTCTTCCATTCCGTTCCTTTTGCTGCAAAAAGCTTATAAAATGCAAAGAAGTCGTGGTTGAGGCTGCCGATGTACCAAAAACCTTGATCAACTATGTTATCTCTAATTCAATTGAGGTTCTGGTACTTGGAGCACCCACAAGAACCGGCCTTTTCAAATCATTCAAAACAACAACAGACGTCCCAAGCTCCGTGTTAAAAGGGATACCAGATTTCTGCACTCTCTATGTCATTGGGAAAGGAAAGATCTCATATATGCAAACTGCTACCACAGCACCACCCCAAAAAGCACATAACCAAATTCAAAAACAATCCAGCAGAATTTCGGAAAATGATGAACAATCCATGCGTAAACAGCATCCCAGGGGACGATCTTCAATGAACCACTCATACGACCTATCACCAGAGAATAGTGATATATCATCTGTGAGCAGCGGAAGACCAAGCATTGATCACATGTTTCCTTCCTTGTACAATAGTGTGTCATCGGGAATGAGCTCCCGACAATCAATGGGCTTGGAGTTTGACAGCATAAGCTCTGCGTCATCTTACTCTAGACACCAATATATTGACATGAGCGCCTCACCATACGGATTCTCAACATCCTCATCGGAAAGTGGAAGATCATGGTCATCGTCACAAAACGTGGATGAAGTGGAAGTTGAGATGAGGAGGCTCCAGCTAGAGCTCAAGCAAACAATGGACATGTATAACGCGGCCTGCAAGGAGGCAGTCGCATCAAAACATACGGCAAACCGATTGAAATCGGAAGAACAACAACGATTACATGAGGCACGAATAGCAGAGGAAGCTGCATTGGCACAAATAGAGAATGAGAAAGCGAAATGTAAGGCAGCCATTCAGGTGGCAGCGGCAGCTCAAATGATTGCTGAACTGGAATCCCAAAAAAGGAGGCTTGCAGAAATGACGATTCTTGGACAAGCTGAAGAGAAGAAGGGAACAGAAGCAACGGCATACAGTTTCAGGTATAGGAAATATACAATTGAGGAGATTGAAGTAGCAACAAATAAATTTTCACAAGATCGCAAGATTGGAGAAGGAGGCTATGGACCAGTGTACTGGGGTGAACTGGATCACACACAAGTAGCAATCAAAATTCTACGTCCAGATGCAGCGCACGGACGTTCACAGTTCCAACAAGAGATCGAAGTATTGAGCTGCATACGACATCCAAACATGGTGCTCCTTCTTGGAGCTTGCCCAGAGTATGGTTGTCTGGTCTATGAGTACATGGCTAAGGGGAGCTTAGAAGACTGTCTCTTCCAGCAAGGTAACAGTAAGACCCCGGCTATTCCTTGGCAGCTCAGGTTCCGAATTGCTGCAGAAATTGGCACTGCGCTTTTGTTCCTTCACCAGACGAAGCCAGAACCAATTGTACACCGTGACCTAAAACCTGGAAACATTTTGTTAGACCATAACTATGTGAGCAAGATTAGTGATGTAGGTCTAGCCAGGCTTGTCCCTCCATCTGTACAAGACTCTGTCACTCAGTACCGAATGACATCAACAGCTGGAACCTTCTGTTATATCGACCCAGAGTATCAACAAACTGGCATGCTTGGAACGAAATCTGATGTCTACTCACTCGGGGTCATGTTTCTACAACTAATAACAGCGAAATCGCCAATGGGTTTGACTCATCATGTCGAGCGAGCTTTGGAGCTAGGGACCTTTGCCGAGTTGCTTGACCCTGCTGTTCCTGACTGGCCAGTGGAGGAAGCCACCAACTTTGCCAAGTTAGCCTTACAATGTGCTGAAATGAGGCGGAAAGACAGACCTGATCTTGGGAAGGTTATGCTACCTGAACTTAACAGATTGAGAGCATTAGCCGAAGATAGCATGCCTGGTGCTGGTGGTGGTGGAGTATACTCACAAAGACATAGCAAAAGCTCGATGTCCTCATGTGATCAAGATGTGCTAAGCGACGTCCATCAACCACTATCTGGGTACGAGAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase Pkinase_Tyr Usp

Protein Analysis

1132

Amino Acids

126.68

Weight (kDa)

5.61

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07020 AT5G35380
fragaria_vesca FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_1g27800 FvH4_6g28460 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29380 FvH4_6g29580 FvH4_6g29580 FvH4_6g29581
malus_domestica MD02G1258200.v1.1 MD09G1226500.v1.1 MD13G1232900.v1.1 MD13G1233000.v1.1 MD17G1212900.v1.1 MD17G1215300.v1.1 MD17G1215400.v1.1 MD17G1224800.v1.1
prunus_persica Prupe.1G064300_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.2G073500_v2.0.a1 Prupe.3G088300_v2.0.a1 Prupe.3G091100_v2.0.a1 Prupe.3G100600_v2.0.a1
pyrus_communis pycom02g21950 pycom09g14550 pycom13g20580 pycom17g21790 pycom17g21970 pycom17g22850
rosa_chinensis RchiOBHm_Chr1g0332091 RchiOBHm_Chr2g0132681 RchiOBHm_Chr2g0134781 RchiOBHm_Chr2g0135161 RchiOBHm_Chr2g0135181 RchiOBHm_Chr2g0135191 RchiOBHm_Chr2g0135201 RchiOBHm_Chr4g0397641 RchiOBHm_Chr4g0397651
rosa_laevigata RLG00000009421 RLG00000019421 RLG00000019446 RLG00000019447 RLG00000029693
rosa_multiflora Rmu_co8345215.1_g000001 Rmu_sc0000100.1_g000004 Rmu_sc0000718.1_g000015 Rmu_sc0002729.1_g000006 Rmu_sc0003358.1_g000008 Rmu_sc0004216.1_g000001 Rmu_sc0013462.1_g000001 Rmu_sc0013462.1_g000002 Rmu_sc0016837.1_g000002 Rmu_sc0021270.1_g000001 Rmu_sc0021634.1_g000001 Rmu_sc0024706.1_g000001
rosa_roxburghii Rroxscaffold_2G00109410 Rroxscaffold_2G00109420 Rroxscaffold_2G00109760 Rroxscaffold_4G00318390 Rroxscaffold_5G00342450 Rroxscaffold_5G00342460
rosa_rugosa Rorug01G0098200 Rorug01G0098300 Rorug02G0306000 Rorug02G0320000 Rorug02G0323800 Rorug02G0323800 Rorug03G0342000 Rorug03G0342100
rosa_samantha Rh1AG006500 Rh1AG122200 Rh1BG093600 Rh1CG116800 Rh2AG359000 Rh2AG371300 Rh2AG371400 Rh2AG373600 Rh2BG365600 Rh2BG377600 Rh2BG380100 Rh2BG380200 Rh2BG380300 Rh2CG342200 Rh2CG356000 Rh2CG359300 Rh2CG359600 Rh2DG382100 Rh2DG394600 Rh2DG396600 Rh2DG396700 Rh2DG396800 Rh2DG396900 Rh4BG077700 Rh4CG085500 Rh4DG072600
rosa_wichuraiana Rw1G009960 Rw2G029220 Rw2G030290 Rw2G030560 Rw2G030570 Rw4G006520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1454
AasI GACNNNNNNGTC 1 cut(s) 436
AatII GACGTC 4 cut(s) 234, 300, 1599, 3366
Acc65I GGTACC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 63
AccB7I CCANNNNNTGG 2 cut(s) 2645, 3380
AccBSI CCGCTC 2 cut(s) 923, 967
AccI GTMKAC 4 cut(s) 300, 424, 3015, 3303
AccII CGCG 2 cut(s) 116, 2132
AclI AACGTT 1 cut(s) 315
AclWI GGATC 3 cut(s) 614, 996, 2517
AcoI YGGCCR 1 cut(s) 3143
AcsI RAATTY 7 cut(s) 382, 744, 839, 1716, 1737, 2447, 2532
AcuI CTGAAG 5 cut(s) 74, 396, 426, 1027, 2382
AcyI GRCGYC 4 cut(s) 231, 297, 1596, 3363
AfiI CCNNNNNNNGG 8 cut(s) 439, 1080, 1363, 2325, 2645, 3221, 3287, 3380
AflIII ACRYGT 3 cut(s) 1303, 1872, 2121
AjnI CCWGG 5 cut(s) 1136, 1778, 2830, 2888, 3280
AjuI GAANNNNNNNTTGG 4 cut(s) 69, 101, 1293, 1325
Alw21I GWGCWC 4 cut(s) 1552, 1916, 2106, 2622
Alw26I GTCTC 4 cut(s) 243, 460, 1236, 2699
AlwI GGATC 3 cut(s) 614, 996, 2517
Ama87I CYCGRG 3 cut(s) 623, 1100, 3023
AoxI GGCC 6 cut(s) 320, 1000, 1297, 1564, 2133, 3143
ApoI RAATTY 7 cut(s) 382, 744, 839, 1716, 1737, 2447, 2532
ArsI GACNNNNNNTTYG 2 cut(s) 130, 162
Asp718I GGTACC 1 cut(s) 63
AspLEI GCGC 4 cut(s) 616, 1997, 2554, 2776
AspS9I GGNCC 6 cut(s) 47, 502, 993, 1001, 2486, 3105
AsuC2I CCSGG 4 cut(s) 500, 696, 1157, 2723
AsuHPI GGTGA 8 cut(s) 22, 226, 1234, 1282, 1809, 1992, 2513, 2780
AvaI CYCGRG 3 cut(s) 623, 1100, 3023
AvaII GGWCC 5 cut(s) 47, 502, 993, 2486, 3105
BaeGI GKGCMC 1 cut(s) 1100
BaeI ACNNNNGTAYC 2 cut(s) 1135, 1168
BalI TGGCCA 1 cut(s) 3145
BanI GGYRCC 1 cut(s) 63
BanII GRGCYC 2 cut(s) 1916, 2106
BbsI GAAGAC 5 cut(s) 666, 758, 1176, 1861, 2694
Bbv12I GWGCWC 4 cut(s) 1552, 1916, 2106, 2622
BccI CCATC 6 cut(s) 101, 275, 526, 1013, 2911, 3375
BceAI ACGGC 2 cut(s) 2181, 2403
BcgI CGANNNNNNTGC 4 cut(s) 1051, 1085, 1450, 1484
BciT130I CCWGG 5 cut(s) 1138, 1780, 2832, 2890, 3282
BciVI GTATCC 2 cut(s) 1363, 1614
BclI TGATCA 3 cut(s) 1503, 1867, 3343
BcnI CCSGG 4 cut(s) 500, 696, 1157, 2723
BcoDI GTCTC 4 cut(s) 243, 460, 1236, 2699
BfaI CTAG 6 cut(s) 509, 635, 1971, 2099, 2885, 3101
BfmI CTRYAG 1 cut(s) 2757
BfoI RGCGCY 2 cut(s) 617, 1998
BfuI GTATCC 2 cut(s) 1363, 1614
BglII AGATCT 1 cut(s) 1662
BlpI GCTNAGC 1 cut(s) 3356
Bme1390I CCNGG 9 cut(s) 500, 696, 1138, 1157, 1780, 2723, 2832, 2890, 3282
Bme18I GGWCC 5 cut(s) 47, 502, 993, 2486, 3105
BmeT110I CYCGRG 3 cut(s) 623, 1100, 3023
BmgT120I GGNCC 6 cut(s) 47, 502, 993, 1001, 2486, 3105
BmiI GGNNCC 6 cut(s) 65, 1003, 2093, 2747, 2953, 3106
BmrFI CCNGG 9 cut(s) 500, 696, 1138, 1157, 1780, 2723, 2832, 2890, 3282
BmrI ACTGGG 2 cut(s) 338, 2506
BmsI GCATC 5 cut(s) 110, 1037, 1783, 2162, 2536
BmuI ACTGGG 2 cut(s) 338, 2506
BpiI GAAGAC 5 cut(s) 666, 758, 1176, 1861, 2694
BpmI CTGGAG 1 cut(s) 2078
Bpu10I CCTNAGC 2 cut(s) 2673, 2741
Bpu1102I GCTNAGC 1 cut(s) 3356
BpuEI CTTGAG 2 cut(s) 944, 2090
BpuMI CCSGG 4 cut(s) 500, 696, 1157, 2723
BsaBI GATNNNNATC 3 cut(s) 123, 449, 2179
BsaHI GRCGYC 4 cut(s) 231, 297, 1596, 3363
BsaI GGTCTC 2 cut(s) 243, 460
BsaJI CCNNGG 6 cut(s) 1156, 1357, 1778, 1779, 2721, 2732
BsaWI WCCGGW 3 cut(s) 469, 482, 1292
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 8 cut(s) 439, 1080, 1363, 2325, 2645, 3221, 3287, 3380
Bse118I RCCGGY 2 cut(s) 1336, 1562
Bse1I ACTGG 9 cut(s) 344, 1391, 2316, 2489, 2501, 2511, 2992, 3146, 3146
Bse8I GATNNNNATC 3 cut(s) 123, 449, 2179
BseBI CCWGG 5 cut(s) 1138, 1780, 2832, 2890, 3282
BseDI CCNNGG 6 cut(s) 1156, 1357, 1778, 1779, 2721, 2732
BseGI GGATG 6 cut(s) 112, 286, 1774, 2018, 2071, 2605
BseJI GATNNNNATC 3 cut(s) 123, 449, 2179
BseLI CCNNNNNNNGG 8 cut(s) 439, 1080, 1363, 2325, 2645, 3221, 3287, 3380
BseMII CTCAG 2 cut(s) 2755, 2939
BseNI ACTGG 9 cut(s) 344, 1391, 2316, 2489, 2501, 2511, 2992, 3146, 3146
BseRI GAGGAG 3 cut(s) 941, 2101, 2438
BseSI GKGCMC 1 cut(s) 1100
BsgI GTGCAG 2 cut(s) 373, 1618
Bsh1236I CGCG 2 cut(s) 116, 2132
BshFI GGCC 6 cut(s) 322, 1002, 1299, 1566, 2135, 3145
BshNI GGYRCC 1 cut(s) 63
BsiHKAI GWGCWC 4 cut(s) 1552, 1916, 2106, 2622
BsiHKCI CYCGRG 3 cut(s) 623, 1100, 3023
BslFI GGGAC 6 cut(s) 965, 1213, 1583, 1797, 2882, 3118
BslI CCNNNNNNNGG 8 cut(s) 439, 1080, 1363, 2325, 2645, 3221, 3287, 3380
BsmAI GTCTC 4 cut(s) 243, 460, 1236, 2699
BsmFI GGGAC 6 cut(s) 965, 1213, 1583, 1797, 2882, 3118
BsnI GGCC 6 cut(s) 322, 1002, 1299, 1566, 2135, 3145
Bso31I GGTCTC 2 cut(s) 243, 460
BsoBI CYCGRG 3 cut(s) 623, 1100, 3023
Bsp1286I GDGCHC 5 cut(s) 1100, 1552, 1916, 2106, 2622
Bsp1407I TGTACA 4 cut(s) 1380, 1887, 2812, 2908
Bsp1720I GCTNAGC 1 cut(s) 3356
BspANI GGCC 6 cut(s) 322, 1002, 1299, 1566, 2135, 3145
BspCNI CTCAG 2 cut(s) 2754, 2938
BspFNI CGCG 2 cut(s) 116, 2132
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 6 cut(s) 65, 1003, 2093, 2747, 2953, 3106
BspMAI CTGCAG 1 cut(s) 2761
BspPI GGATC 3 cut(s) 614, 996, 2517
BspT107I GGYRCC 1 cut(s) 63
BspTNI GGTCTC 2 cut(s) 243, 460
BsrBI CCGCTC 2 cut(s) 923, 967
BsrFI RCCGGY 2 cut(s) 1336, 1562
BsrGI TGTACA 4 cut(s) 1380, 1887, 2812, 2908
BsrI ACTGG 9 cut(s) 344, 1391, 2316, 2489, 2501, 2511, 2992, 3146, 3146
BssAI RCCGGY 2 cut(s) 1336, 1562
BssECI CCNNGG 6 cut(s) 1156, 1357, 1778, 1779, 2721, 2732
BssNAI GTATAC 1 cut(s) 3304
BssNI GRCGYC 4 cut(s) 231, 297, 1596, 3363
BssT1I CCWWGG 2 cut(s) 1357, 2732
Bst1107I GTATAC 1 cut(s) 3304
Bst2UI CCWGG 5 cut(s) 1138, 1780, 2832, 2890, 3282
Bst4CI ACNGT 7 cut(s) 189, 1204, 2396, 2568, 2693, 2714, 2819
Bst6I CTCTTC 6 cut(s) 261, 497, 552, 1189, 2358, 2702
BstACI GRCGYC 4 cut(s) 231, 297, 1596, 3363
BstAUI TGTACA 4 cut(s) 1380, 1887, 2812, 2908
BstDEI CTNAG 5 cut(s) 2673, 2683, 2741, 2925, 3356
BstF5I GGATG 6 cut(s) 112, 286, 1774, 2018, 2071, 2605
BstFNI CGCG 2 cut(s) 116, 2132
BstH2I RGCGCY 2 cut(s) 617, 1998
BstHHI GCGC 4 cut(s) 616, 1997, 2554, 2776
BstMAI GTCTC 4 cut(s) 243, 460, 1236, 2699
BstNI CCWGG 5 cut(s) 1138, 1780, 2832, 2890, 3282
BstNSI RCATGY 6 cut(s) 1262, 1307, 1876, 2125, 2995, 3280
BstSCI CCNGG 9 cut(s) 498, 694, 1136, 1155, 1778, 2721, 2830, 2888, 3280
BstSFI CTRYAG 1 cut(s) 2757
BstSLI GKGCMC 1 cut(s) 1100
BstUI CGCG 2 cut(s) 116, 2132
BstV2I GAAGAC 5 cut(s) 666, 758, 1176, 1861, 2694
BstX2I RGATCY 2 cut(s) 619, 1662
BstXI CCANNNNNNTGG 2 cut(s) 2281, 2496
BstYI RGATCY 2 cut(s) 619, 1662
BstZ17I GTATAC 1 cut(s) 3304
BsuI GTATCC 2 cut(s) 1363, 1614
BsuRI GGCC 6 cut(s) 322, 1002, 1299, 1566, 2135, 3145
BtgZI GCGATG 2 cut(s) 300, 1054
BtsCI GGATG 6 cut(s) 112, 286, 1774, 2018, 2071, 2605
BtsI GCAGTG 3 cut(s) 534, 885, 2769
BtsIMutI CAGTG 5 cut(s) 534, 885, 2496, 2769, 3153
CciI TCATGA 1 cut(s) 943
CfoI GCGC 4 cut(s) 616, 1997, 2554, 2776
Cfr10I RCCGGY 2 cut(s) 1336, 1562
Cfr13I GGNCC 6 cut(s) 47, 502, 993, 1001, 2486, 3105
CpoI CGGWCCG 1 cut(s) 47
CseI GACGC 3 cut(s) 671, 946, 1947
CspCI CAANNNNNGTGG 6 cut(s) 836, 871, 1121, 1156, 3148, 3183
CspI CGGWCCG 1 cut(s) 47
DdeI CTNAG 5 cut(s) 2673, 2683, 2741, 2925, 3356
DrdI GACNNNNNNGTC 1 cut(s) 436
DseDI GACNNNNNNGTC 1 cut(s) 436
EaeI YGGCCR 1 cut(s) 3143
Eam1104I CTCTTC 6 cut(s) 261, 497, 552, 1189, 2358, 2702
EarI CTCTTC 6 cut(s) 261, 497, 552, 1189, 2358, 2702
EciI GGCGGA 2 cut(s) 681, 3217
Ecl136II GAGCTC 2 cut(s) 1914, 2104
Eco130I CCWWGG 2 cut(s) 1357, 2732
Eco147I AGGCCT 1 cut(s) 322
Eco24I GRGCYC 2 cut(s) 1916, 2106
Eco31I GGTCTC 2 cut(s) 243, 460
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 5 cut(s) 47, 502, 993, 2486, 3105
Eco53kI GAGCTC 2 cut(s) 1914, 2104
Eco57I CTGAAG 5 cut(s) 74, 396, 426, 1027, 2382
Eco88I CYCGRG 3 cut(s) 623, 1100, 3023
EcoICRI GAGCTC 2 cut(s) 1914, 2104
EcoO109I RGGNCCY 1 cut(s) 3105
EcoRI GAATTC 2 cut(s) 744, 839
EcoRII CCWGG 5 cut(s) 1136, 1778, 2830, 2888, 3280
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 2 cut(s) 1357, 2732
EcoT22I ATGCAT 1 cut(s) 103
EcoT38I GRGCYC 2 cut(s) 1916, 2106
ErhI CCWWGG 2 cut(s) 1357, 2732
FalI AAGNNNNNCTT 6 cut(s) 953, 985, 2666, 2698, 3164, 3196
FaqI GGGAC 6 cut(s) 965, 1213, 1583, 1797, 2882, 3118
FauI CCCGC 1 cut(s) 1046
FbaI TGATCA 3 cut(s) 1503, 1867, 3343
FblI GTMKAC 4 cut(s) 300, 424, 3015, 3303
FokI GGATG 6 cut(s) 119, 293, 1761, 2005, 2078, 2592
FriOI GRGCYC 2 cut(s) 1916, 2106
FspBI CTAG 6 cut(s) 509, 635, 1971, 2099, 2885, 3101
GlaI GCGC 4 cut(s) 615, 1996, 2553, 2775
GsuI CTGGAG 1 cut(s) 2078
HaeII RGCGCY 2 cut(s) 617, 1998
HaeIII GGCC 6 cut(s) 322, 1002, 1299, 1566, 2135, 3145
HgaI GACGC 3 cut(s) 671, 946, 1947
HhaI GCGC 4 cut(s) 616, 1997, 2554, 2776
Hin1I GRCGYC 4 cut(s) 231, 297, 1596, 3363
Hin6I GCGC 4 cut(s) 614, 1995, 2552, 2774
HinP1I GCGC 4 cut(s) 614, 1995, 2552, 2774
HincII GTYRAC 3 cut(s) 301, 1256, 1396
HindII GTYRAC 3 cut(s) 301, 1256, 1396
HindIII AAGCTT 4 cut(s) 408, 890, 1011, 1448
HphI GGTGA 8 cut(s) 22, 226, 1234, 1282, 1809, 1992, 2513, 2780
Hpy188III TCNNGA 9 cut(s) 623, 944, 1212, 1905, 1917, 1971, 2543, 3137, 3347
Hpy99I CGWCG 5 cut(s) 233, 299, 302, 305, 3365
HpyCH4III ACNGT 7 cut(s) 189, 1204, 2396, 2568, 2693, 2714, 2819
HpyCH4IV ACGT 9 cut(s) 222, 231, 297, 315, 1596, 2061, 2539, 2560, 3363
HpyF3I CTNAG 5 cut(s) 2673, 2683, 2741, 2925, 3356
HpySE526I ACGT 9 cut(s) 222, 231, 297, 315, 1596, 2061, 2539, 2560, 3363
Hsp92I GRCGYC 4 cut(s) 231, 297, 1596, 3363
HspAI GCGC 4 cut(s) 614, 1995, 2552, 2774
KpnI GGTACC 1 cut(s) 67
KroI GCCGGC 1 cut(s) 1336
KroNI GCCGGC 1 cut(s) 1338
Ksp22I TGATCA 3 cut(s) 1503, 1867, 3343
LweI GCATC 5 cut(s) 110, 1037, 1783, 2162, 2536
MaeI CTAG 6 cut(s) 509, 635, 1971, 2099, 2885, 3101
MaeII ACGT 9 cut(s) 222, 231, 297, 315, 1596, 2061, 2539, 2560, 3363
MaeIII GTNAC 8 cut(s) 202, 232, 1222, 1288, 2052, 2708, 2819, 2920
MbiI CCGCTC 2 cut(s) 923, 967
MfeI CAATTG 3 cut(s) 1527, 2418, 2808
MflI RGATCY 2 cut(s) 619, 1662
MhlI GDGCHC 5 cut(s) 1100, 1552, 1916, 2106, 2622
MlsI TGGCCA 1 cut(s) 3145
MluNI TGGCCA 1 cut(s) 3145
MlyI GAGTC 4 cut(s) 278, 987, 2909, 3067
MmeI TCCRAC 3 cut(s) 420, 966, 2596
Mox20I TGGCCA 1 cut(s) 3145
Mph1103I ATGCAT 1 cut(s) 103
MreI CGCCGGCG 1 cut(s) 1336
MroNI GCCGGC 1 cut(s) 1336
MscI TGGCCA 1 cut(s) 3145
MseI TTAA 4 cut(s) 369, 476, 1613, 3246
MslI CAYNNNNRTG 2 cut(s) 2279, 2856
Msp20I TGGCCA 1 cut(s) 3145
MspA1I CMGCKG 4 cut(s) 1053, 1851, 2288, 2948
MspR9I CCNGG 9 cut(s) 500, 696, 1138, 1157, 1780, 2723, 2832, 2890, 3282
MunI CAATTG 3 cut(s) 1527, 2418, 2808
MvaI CCWGG 5 cut(s) 1138, 1780, 2832, 2890, 3282
MvnI CGCG 2 cut(s) 116, 2132
NaeI GCCGGC 1 cut(s) 1338
NciI CCSGG 4 cut(s) 500, 696, 1157, 2723
NgoMIV GCCGGC 1 cut(s) 1336
NlaIV GGNNCC 6 cut(s) 65, 1003, 2093, 2747, 2953, 3106
NmeAIII GCCGAG 1 cut(s) 3139
NmuCI GTSAC 7 cut(s) 202, 232, 1222, 1288, 2052, 2819, 2920
NsiI ATGCAT 1 cut(s) 103
NspI RCATGY 6 cut(s) 1262, 1307, 1876, 2125, 2995, 3280
PaeI GCATGC 2 cut(s) 2995, 3280
PagI TCATGA 1 cut(s) 943
PasI CCCWGGG 1 cut(s) 1779
PceI AGGCCT 1 cut(s) 322
PciI ACATGT 3 cut(s) 1303, 1872, 2121
PcsI WCGNNNNNNNCGW 3 cut(s) 228, 234, 300
PdiI GCCGGC 1 cut(s) 1338
PfeI GAWTC 9 cut(s) 119, 377, 418, 596, 707, 1208, 2010, 2315, 2347
PflFI GACNNNGTC 1 cut(s) 2918
PflMI CCANNNNNTGG 2 cut(s) 2645, 3380
PleI GAGTC 4 cut(s) 277, 986, 2909, 3067
PpsI GAGTC 4 cut(s) 277, 986, 2909, 3067
PpuMI RGGWCCY 1 cut(s) 3105
PscI ACATGT 3 cut(s) 1303, 1872, 2121
PsiI TTATAA 1 cut(s) 1454
Psp124BI GAGCTC 2 cut(s) 1916, 2106
Psp1406I AACGTT 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 3105
Psp6I CCWGG 5 cut(s) 1136, 1778, 2830, 2888, 3280
PspGI CCWGG 5 cut(s) 1136, 1778, 2830, 2888, 3280
PspN4I GGNNCC 6 cut(s) 65, 1003, 2093, 2747, 2953, 3106
PspPI GGNCC 6 cut(s) 47, 502, 993, 1001, 2486, 3105
PspPPI RGGWCCY 1 cut(s) 3105
PsrI GAACNNNNNNTAC 4 cut(s) 1790, 1822, 2796, 2828
PstI CTGCAG 1 cut(s) 2761
PsuI RGATCY 2 cut(s) 619, 1662
PsyI GACNNNGTC 1 cut(s) 2918
PvuII CAGCTG 1 cut(s) 2948
RseI CAYNNNNRTG 2 cut(s) 2279, 2856
Rsr2I CGGWCCG 1 cut(s) 47
RsrII CGGWCCG 1 cut(s) 47
SacI GAGCTC 2 cut(s) 1916, 2106
SalI GTCGAC 1 cut(s) 299
SaqAI TTAA 4 cut(s) 369, 476, 1613, 3246
Sau96I GGNCC 6 cut(s) 47, 502, 993, 1001, 2486, 3105
SchI GAGTC 4 cut(s) 278, 987, 2909, 3067
ScrFI CCNGG 9 cut(s) 500, 696, 1138, 1157, 1780, 2723, 2832, 2890, 3282
SduI GDGCHC 5 cut(s) 1100, 1552, 1916, 2106, 2622
SfaNI GCATC 5 cut(s) 110, 1037, 1783, 2162, 2536
SfcI CTRYAG 1 cut(s) 2757
SgrAI CRCCGGYG 1 cut(s) 1336
SgrDI CGTCGACG 1 cut(s) 299
SinI GGWCC 5 cut(s) 47, 502, 993, 2486, 3105
SmiMI CAYNNNNRTG 2 cut(s) 2279, 2856
SmlI CTYRAG 2 cut(s) 959, 2105
SmoI CTYRAG 2 cut(s) 959, 2105
SphI GCATGC 2 cut(s) 2995, 3280
SseBI AGGCCT 1 cut(s) 322
SspMI CTAG 6 cut(s) 509, 635, 1971, 2099, 2885, 3101
SstI GAGCTC 2 cut(s) 1916, 2106
StuI AGGCCT 1 cut(s) 322
StyD4I CCNGG 9 cut(s) 498, 694, 1136, 1155, 1778, 2721, 2830, 2888, 3280
StyI CCWWGG 2 cut(s) 1357, 2732
TaaI ACNGT 7 cut(s) 189, 1204, 2396, 2568, 2693, 2714, 2819
TaiI ACGT 9 cut(s) 225, 234, 300, 318, 1599, 2064, 2542, 2563, 3366
TaqII GACCGA 2 cut(s) 64, 981
TatI WGTACW 7 cut(s) 1104, 1380, 1887, 2493, 2664, 2812, 2908
TauI GCSGC 3 cut(s) 967, 2135, 2291
TfiI GAWTC 9 cut(s) 119, 377, 418, 596, 707, 1208, 2010, 2315, 2347
Tru1I TTAA 4 cut(s) 369, 476, 1613, 3246
Tru9I TTAA 4 cut(s) 369, 476, 1613, 3246
TscAI CASTG 5 cut(s) 534, 885, 2496, 2776, 3153
TseFI GTSAC 7 cut(s) 202, 232, 1222, 1288, 2052, 2819, 2920
Tsp45I GTSAC 7 cut(s) 202, 232, 1222, 1288, 2052, 2819, 2920
TspGWI ACGGA 7 cut(s) 179, 221, 389, 1418, 1597, 2022, 2571
TspRI CASTG 5 cut(s) 534, 885, 2496, 2776, 3153
Tth111I GACNNNGTC 1 cut(s) 2918
Van91I CCANNNNNTGG 2 cut(s) 2645, 3380
VpaK11BI GGWCC 5 cut(s) 47, 502, 993, 2486, 3105
XapI RAATTY 7 cut(s) 382, 744, 839, 1716, 1737, 2447, 2532
XbaI TCTAGA 1 cut(s) 1970
XceI RCATGY 6 cut(s) 1262, 1307, 1876, 2125, 2995, 3280
XmiI GTMKAC 4 cut(s) 300, 424, 3015, 3303
XspI CTAG 6 cut(s) 509, 635, 1971, 2099, 2885, 3101
ZraI GACGTC 4 cut(s) 232, 298, 1597, 3364
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.