FvH4_2g21230

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000399
Physical Location & Seq
Forward (+)
17649245 .. 17650614
1370 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g21230.t1

Sequence Viewer

Length: 1041 bp
ATGGCGAATTACCTCGACGTCTCTCTCTGCGAAAGGCTGACGGTCGATCAAGGTCCTCCTATTATGCTTCCGAAGAGGCTTGATGAGGCTGTCGTTCAGTTTTACACCCTCACAAACGGTCAGACCTACAGCTCCGATCTCCCGGAAACCAGGGGTAAGGTCTGCTTTGCTTCTCTAGGATGGCTAGTTACTCTAACCAGGGTTGAAGGGCAAGTGAAACTTGATCTGTTCCACCCATCAAATCGTGCCGAAATCAAACTACCAGATGGTGTGACCTATCCGCTCGGTAACATACGCAAGTTTGTGTTGTCGTCGAATCCTGCTTCGACATTGGATTATACAGTTATGTTTCAGACCTTCTCTTCCTTGGCATATTGCAGACCAGGACTTGGCCAAGGTTGGACAAAGCTCATGTTTAACGGACACAATTACTTTAGGGACCTCACATACTATCAAGGAAACTTGTATGTTGTGGAACAATCCGGCAAAGTCTCTGTTGTTTGCGACATTGAAGACCCTGAGCGAGCATATTTGAGTGTTGTTGCTCCAGAGATTCCTGATCAGGAGAAGCTTCTAGGTCGTATGAAGGGCATCAAAGATCTATATCTGGTGGAATCCGCAGGGTCCCTTTTGGTGGTTCTGTGTGTTTTCTTACATCATCAGTCTGAAACAACTGTTGGCTTCAGGGTTTACAAGGTCCCGTTCAGTAATGGGGAGTCATGGTCCGACTCAGAGGTCAAGGATTTGGGAAATAGAGCCCTCTTCTTGAGTAAAAGCAGCTCTTCCTTCTCCATAGAGGTCTCGGGGGAAAACTCTGGATTATGCAAGCCCAATTGCATATATTTCATGAACAGCAAAACGACCTCTCCATACCTTGACTTGGATGTTGGTGTTTTCAACATGGAGGATAAGACCATTGATCGAGACTTCCGCAATGCCTTCAAACACAGGACTAAAGAACTCAGAGAAGTGACAGAACTCGTATGCAATCCCAAAATCAAAGAACCCCTTCTATGGATTCAACCGACCCTCTGCTCTTGA

Protein Analysis

347

Amino Acids

39.01

Weight (kDa)

5.83

Isoelectric Point (pI)

30.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 34 - 300 1.2e-47 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 734
AatII GACGTC 1 cut(s) 21
AccB7I CCANNNNNTGG 1 cut(s) 389
AccBSI CCGCTC 1 cut(s) 283
AciI CCGC 3 cut(s) 281, 618, 931
AcoI YGGCCR 1 cut(s) 391
AcuI CTGAAG 1 cut(s) 667
AcyI GRCGYC 1 cut(s) 18
AfiI CCNNNNNNNGG 4 cut(s) 389, 634, 880, 1014
AgsI TTSAA 5 cut(s) 206, 512, 898, 943, 1022
AjnI CCWGG 3 cut(s) 149, 197, 382
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 4 cut(s) 132, 409, 571, 780
AluI AGCT 4 cut(s) 132, 409, 571, 780
Alw26I GTCTC 4 cut(s) 25, 496, 805, 918
Ama87I CYCGRG 1 cut(s) 802
AoxI GGCC 1 cut(s) 391
ApeKI GCWGC 1 cut(s) 777
Asp700I GAANNNNTTC 1 cut(s) 1008
AspS9I GGNCC 5 cut(s) 53, 439, 624, 697, 723
AsuC2I CCSGG 1 cut(s) 143
AvaI CYCGRG 1 cut(s) 802
AvaII GGWCC 5 cut(s) 53, 439, 624, 697, 723
BalI TGGCCA 1 cut(s) 393
BanII GRGCYC 1 cut(s) 760
BbsI GAAGAC 1 cut(s) 519
BbvI GCAGC 1 cut(s) 789
BccI CCATC 3 cut(s) 174, 244, 260
BciT130I CCWGG 3 cut(s) 151, 199, 384
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 1 cut(s) 143
BcoDI GTCTC 4 cut(s) 25, 496, 805, 918
BfaI CTAG 3 cut(s) 176, 185, 575
BfmI CTRYAG 1 cut(s) 127
BglII AGATCT 1 cut(s) 598
BisI GCNGC 1 cut(s) 778
BlsI GCNGC 1 cut(s) 779
Bme1390I CCNGG 4 cut(s) 143, 151, 199, 384
Bme18I GGWCC 5 cut(s) 53, 439, 624, 697, 723
BmeT110I CYCGRG 1 cut(s) 802
BmgT120I GGNCC 5 cut(s) 53, 439, 624, 697, 723
BmiI GGNNCC 4 cut(s) 440, 625, 626, 699
BmrFI CCNGG 4 cut(s) 143, 151, 199, 384
BmsI GCATC 1 cut(s) 600
BpiI GAAGAC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 787
BpuMI CCSGG 1 cut(s) 143
BsaBI GATNNNNATC 1 cut(s) 603
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 805
BsaJI CCNNGG 4 cut(s) 150, 198, 366, 394
BsaXI ACNNNNNCTCC 4 cut(s) 707, 737, 850, 880
Bsc4I CCNNNNNNNGG 4 cut(s) 389, 634, 880, 1014
Bse3DI GCAATG 1 cut(s) 940
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 3 cut(s) 151, 199, 384
BseDI CCNNGG 4 cut(s) 150, 198, 366, 394
BseGI GGATG 2 cut(s) 185, 889
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 4 cut(s) 389, 634, 880, 1014
BseMI GCAATG 1 cut(s) 940
BseMII CTCAG 3 cut(s) 510, 744, 976
BseXI GCAGC 1 cut(s) 789
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 1 cut(s) 393
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 802
BsiSI CCGG 2 cut(s) 143, 483
BslFI GGGAC 3 cut(s) 452, 610, 683
BslI CCNNNNNNNGG 4 cut(s) 389, 634, 880, 1014
BsmAI GTCTC 4 cut(s) 25, 496, 805, 918
BsmBI CGTCTC 1 cut(s) 25
BsmFI GGGAC 3 cut(s) 452, 610, 683
BsnI GGCC 1 cut(s) 393
Bso31I GGTCTC 1 cut(s) 805
BsoBI CYCGRG 1 cut(s) 802
Bsp1286I GDGCHC 1 cut(s) 760
Bsp143I GATC 6 cut(s) 46, 136, 223, 559, 598, 919
BspACI CCGC 3 cut(s) 281, 618, 931
BspANI GGCC 1 cut(s) 393
BspCNI CTCAG 3 cut(s) 511, 743, 975
BspHI TCATGA 1 cut(s) 846
BspLI GGNNCC 4 cut(s) 440, 625, 626, 699
BspQI GCTCTTC 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 805
BsrBI CCGCTC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 940
BssECI CCNNGG 4 cut(s) 150, 198, 366, 394
BssMI GATC 6 cut(s) 46, 136, 223, 559, 598, 919
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 366, 394
Bst2UI CCWGG 3 cut(s) 151, 199, 384
Bst4CI ACNGT 4 cut(s) 43, 119, 343, 676
Bst6I CTCTTC 4 cut(s) 68, 367, 767, 787
BstACI GRCGYC 1 cut(s) 18
BstC8I GCNNGC 2 cut(s) 525, 827
BstDEI CTNAG 3 cut(s) 519, 730, 962
BstF5I GGATG 2 cut(s) 185, 889
BstKTI GATC 6 cut(s) 49, 139, 226, 562, 601, 922
BstMAI GTCTC 4 cut(s) 25, 496, 805, 918
BstMBI GATC 6 cut(s) 46, 136, 223, 559, 598, 919
BstMCI CGRYCG 1 cut(s) 45
BstNI CCWGG 3 cut(s) 151, 199, 384
BstSCI CCNGG 4 cut(s) 141, 149, 197, 382
BstSFI CTRYAG 1 cut(s) 127
BstV1I GCAGC 1 cut(s) 789
BstV2I GAAGAC 1 cut(s) 519
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 1 cut(s) 393
BtsCI GGATG 2 cut(s) 185, 889
Cac8I GCNNGC 2 cut(s) 525, 827
CciI TCATGA 1 cut(s) 846
Cfr13I GGNCC 5 cut(s) 53, 439, 624, 697, 723
CviAII CATG 4 cut(s) 412, 720, 847, 901
DdeI CTNAG 3 cut(s) 519, 730, 962
DpnI GATC 6 cut(s) 48, 138, 225, 561, 600, 921
DpnII GATC 6 cut(s) 46, 136, 223, 559, 598, 919
DrdI GACNNNNNNGTC 1 cut(s) 734
DseDI GACNNNNNNGTC 1 cut(s) 734
EaeI YGGCCR 1 cut(s) 391
Eam1104I CTCTTC 4 cut(s) 68, 367, 767, 787
EarI CTCTTC 4 cut(s) 68, 367, 767, 787
Eco130I CCWWGG 2 cut(s) 366, 394
Eco24I GRGCYC 1 cut(s) 760
Eco31I GGTCTC 1 cut(s) 805
Eco47I GGWCC 5 cut(s) 53, 439, 624, 697, 723
Eco57I CTGAAG 1 cut(s) 667
Eco88I CYCGRG 1 cut(s) 802
EcoO109I RGGNCCY 4 cut(s) 53, 439, 624, 697
EcoRII CCWGG 3 cut(s) 149, 197, 382
EcoT14I CCWWGG 2 cut(s) 366, 394
EcoT38I GRGCYC 1 cut(s) 760
ErhI CCWWGG 2 cut(s) 366, 394
Esp3I CGTCTC 1 cut(s) 25
FaeI CATG 4 cut(s) 415, 723, 850, 904
FalI AAGNNNNNCTT 8 cut(s) 149, 181, 204, 236, 766, 798, 993, 1025
FaqI GGGAC 3 cut(s) 452, 610, 683
FatI CATG 4 cut(s) 411, 719, 846, 900
FbaI TGATCA 1 cut(s) 559
Fnu4HI GCNGC 1 cut(s) 778
FokI GGATG 2 cut(s) 192, 896
FriOI GRGCYC 1 cut(s) 760
Fsp4HI GCNGC 1 cut(s) 778
FspBI CTAG 3 cut(s) 176, 185, 575
GluI GCNGC 1 cut(s) 778
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 1 cut(s) 393
HapII CCGG 2 cut(s) 143, 483
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 4 cut(s) 415, 723, 850, 904
HindIII AAGCTT 1 cut(s) 569
HinfI GANTC 6 cut(s) 316, 553, 614, 716, 728, 1018
HpaII CCGG 2 cut(s) 143, 483
Hpy166II GTNNAC 1 cut(s) 691
Hpy188I TCNGA 8 cut(s) 72, 123, 136, 354, 667, 727, 733, 965
Hpy188III TCNNGA 8 cut(s) 548, 557, 563, 766, 816, 847, 923, 1038
Hpy8I GTNNAC 1 cut(s) 691
Hpy99I CGWCG 2 cut(s) 20, 316
HpyAV CCTTC 6 cut(s) 200, 367, 580, 796, 949, 1019
HpyCH4III ACNGT 4 cut(s) 43, 119, 343, 676
HpyCH4IV ACGT 1 cut(s) 18
HpyCH4V TGCA 4 cut(s) 378, 825, 837, 987
HpyF3I CTNAG 3 cut(s) 519, 730, 962
HpySE526I ACGT 1 cut(s) 18
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 4 cut(s) 415, 723, 850, 904
KflI GGGWCCC 1 cut(s) 624
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 6 cut(s) 46, 136, 223, 559, 598, 919
LguI GCTCTTC 1 cut(s) 787
LmnI GCTCC 2 cut(s) 137, 550
Lsp1109I GCAGC 1 cut(s) 789
LweI GCATC 1 cut(s) 600
MaeI CTAG 3 cut(s) 176, 185, 575
MaeII ACGT 1 cut(s) 18
MaeIII GTNAC 4 cut(s) 187, 271, 287, 970
MalI GATC 6 cut(s) 48, 138, 225, 561, 600, 921
MbiI CCGCTC 1 cut(s) 283
MboI GATC 6 cut(s) 46, 136, 223, 559, 598, 919
MboII GAAGA 5 cut(s) 85, 354, 524, 754, 774
MfeI CAATTG 1 cut(s) 832
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 760
MlsI TGGCCA 1 cut(s) 393
MluCI AATT 3 cut(s) 7, 427, 832
MluNI TGGCCA 1 cut(s) 393
MlyI GAGTC 2 cut(s) 722, 725
MmeI TCCRAC 2 cut(s) 380, 750
Mox20I TGGCCA 1 cut(s) 393
MroXI GAANNNNTTC 1 cut(s) 1008
MscI TGGCCA 1 cut(s) 393
MseI TTAA 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 393
MspI CCGG 2 cut(s) 143, 483
MspR9I CCNGG 4 cut(s) 143, 151, 199, 384
MunI CAATTG 1 cut(s) 832
MvaI CCWGG 3 cut(s) 151, 199, 384
NciI CCSGG 1 cut(s) 143
NdeII GATC 6 cut(s) 46, 136, 223, 559, 598, 919
NlaIII CATG 4 cut(s) 415, 723, 850, 904
NlaIV GGNNCC 4 cut(s) 440, 625, 626, 699
NmuCI GTSAC 2 cut(s) 271, 970
PagI TCATGA 1 cut(s) 846
PciSI GCTCTTC 1 cut(s) 787
PdmI GAANNNNTTC 1 cut(s) 1008
PfeI GAWTC 4 cut(s) 316, 553, 614, 1018
PflMI CCANNNNNTGG 1 cut(s) 389
PfoI TCCNGGA 1 cut(s) 141
PkrI GCNGC 1 cut(s) 779
PleI GAGTC 2 cut(s) 722, 724
PpsI GAGTC 2 cut(s) 722, 724
PpuMI RGGWCCY 4 cut(s) 53, 439, 624, 697
Psp5II RGGWCCY 4 cut(s) 53, 439, 624, 697
Psp6I CCWGG 3 cut(s) 149, 197, 382
PspGI CCWGG 3 cut(s) 149, 197, 382
PspN4I GGNNCC 4 cut(s) 440, 625, 626, 699
PspPI GGNCC 5 cut(s) 53, 439, 624, 697, 723
PspPPI RGGWCCY 4 cut(s) 53, 439, 624, 697
PsuI RGATCY 1 cut(s) 598
SapI GCTCTTC 1 cut(s) 787
SaqAI TTAA 1 cut(s) 417
SatI GCNGC 1 cut(s) 778
Sau3AI GATC 6 cut(s) 46, 136, 223, 559, 598, 919
Sau96I GGNCC 5 cut(s) 53, 439, 624, 697, 723
SchI GAGTC 2 cut(s) 722, 725
ScrFI CCNGG 4 cut(s) 143, 151, 199, 384
SduI GDGCHC 1 cut(s) 760
SfaNI GCATC 1 cut(s) 600
SfcI CTRYAG 1 cut(s) 127
SinI GGWCC 5 cut(s) 53, 439, 624, 697, 723
SmlI CTYRAG 1 cut(s) 766
SmoI CTYRAG 1 cut(s) 766
Sse9I AATT 3 cut(s) 7, 427, 832
SsiI CCGC 3 cut(s) 281, 618, 931
SspMI CTAG 3 cut(s) 176, 185, 575
StyD4I CCNGG 4 cut(s) 141, 149, 197, 382
StyI CCWWGG 2 cut(s) 366, 394
TaaI ACNGT 4 cut(s) 43, 119, 343, 676
TaiI ACGT 1 cut(s) 21
TaqI TCGA 5 cut(s) 15, 45, 314, 326, 922
TasI AATT 3 cut(s) 7, 427, 832
TfiI GAWTC 4 cut(s) 316, 553, 614, 1018
Tru1I TTAA 1 cut(s) 417
Tru9I TTAA 1 cut(s) 417
TseFI GTSAC 2 cut(s) 271, 970
TseI GCWGC 1 cut(s) 777
Tsp45I GTSAC 2 cut(s) 271, 970
TspDTI ATGAA 3 cut(s) 599, 835, 863
TspGWI ACGGA 1 cut(s) 435
Van91I CCANNNNNTGG 1 cut(s) 389
VpaK11BI GGWCC 5 cut(s) 53, 439, 624, 697, 723
XmnI GAANNNNTTC 1 cut(s) 1008
XspI CTAG 3 cut(s) 176, 185, 575
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.