Rw5G020460

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000399
Physical Location & Seq
Forward (+)
26934638 .. 26935704
1067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G020460.1

Sequence Viewer

Length: 1017 bp
ATGTCTTCATTGGCTAACTGGTCAGATCTTCCTCAAGATCTTGCTATCTCTATTGCTAAGCTGATGGTTTGCATGGAGGACTTTTTATCTTTTGGGGCGGTCTGTAAATCATGGAGATCCACTGCCACAAAAGAAAACTTTACTGGTGGATTAAAACATCAAGTTCCGTTCCTTATATTACCCGAGAAGGAGGGCATGGCCAATCGTGATAAGGGTCAAACGTTGTTTTCTTCTCTTGGATGGCTGATAACTGAATCAAAAGATGCAAAAGTTTTAACGCTATTGCATCCTTTAAATCATACCCAAATCAAGTTACCAGAGAACTTCCGATACGAATTTATAAAGTCGTTTGCCTTATCAGCAAGTCCTTCCTTGACATCGGATTTCGTTGTCATGGTTCTTTATCATTGCCGCAGGGGATTGGCTTTTTGCAGAGCAAAGGACAACGAATGGACTAGAGTGGATGCAACGAAAAGGGGAAACAATTCCTACTTTGATCTCACTTATTATAGGGAGCAATTTTATGCCTTGGATCTTCATGGCCGTGTTTTGGTTTGTGACATTGAAGACCCTAAGAAAGCAAAAACGCGGGTAGTTGTTCCACAGATACCAAGGGATACAAGTTTTGAAGATTATCCGACCAGATTATACCTGGTGGAATCAGCAGGAACCTTACTTGTTGTACGAGTGGGCTATCAAGATAAAACCATTGCGTTTAGGGTTTTTGAGGTGCCATTTAGCAATTGCGATTGGTCATACTCCGAAGTTAAGAATTTGGGAAATAGAACCTTGTTCTTGAATGTTTTAGGTTCTTCCTTTTCAATTGAGGCTTCAAACTACTCCGGCGTCAAGTCCAATTGCATATACTTCAGATCTATTCTGTTCAGTTTGGACGAAGAAGCGGGCTCGGGCATGTGTATCTTTGATACGGAAGATGAAAAAAGTGACTTGCGTGGGGTTTTTATAAACTCTTCTAGTTTTGCAATGTCCTATTTATGGATCCAACCCAGTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

338

Amino Acids

38.45

Weight (kDa)

6.25

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 70 - 309 9.2e-49 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 341, 965
AccB1I GGYRCC 1 cut(s) 730
AccII CGCG 1 cut(s) 589
AciI CCGC 4 cut(s) 98, 412, 589, 902
AclI AACGTT 1 cut(s) 221
AclWI GGATC 4 cut(s) 111, 540, 994, 1007
AcoI YGGCCR 2 cut(s) 198, 541
AcsI RAATTY 2 cut(s) 335, 772
AcuI CTGAAG 1 cut(s) 853
AcyI GRCGYC 1 cut(s) 846
AfaI GTAC 1 cut(s) 684
AfiI CCNNNNNNNGG 2 cut(s) 550, 996
AgsI TTSAA 5 cut(s) 566, 629, 799, 822, 834
AjnI CCWGG 1 cut(s) 651
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
AlwI GGATC 4 cut(s) 111, 540, 994, 1007
Ama87I CYCGRG 2 cut(s) 182, 907
AoxI GGCC 2 cut(s) 198, 541
ApoI RAATTY 2 cut(s) 335, 772
Asp700I GAANNNNTTC 1 cut(s) 484
AvaI CYCGRG 2 cut(s) 182, 907
BalI TGGCCA 1 cut(s) 200
BamHI GGATCC 1 cut(s) 999
BanI GGYRCC 1 cut(s) 730
BanII GRGCYC 1 cut(s) 908
BbsI GAAGAC 1 cut(s) 573
BccI CCATC 2 cut(s) 58, 234
BceAI ACGGC 1 cut(s) 528
BciT130I CCWGG 1 cut(s) 653
BciVI GTATCC 1 cut(s) 610
BfaI CTAG 2 cut(s) 456, 975
BfuI GTATCC 1 cut(s) 610
BglII AGATCT 3 cut(s) 25, 37, 872
BisI GCNGC 1 cut(s) 412
BlpI GCTNAGC 1 cut(s) 57
BlsI GCNGC 1 cut(s) 413
Bme1390I CCNGG 1 cut(s) 653
BmeT110I CYCGRG 2 cut(s) 182, 907
BmiI GGNNCC 3 cut(s) 670, 732, 1001
BmrFI CCNGG 1 cut(s) 653
BmrI ACTGGG 1 cut(s) 1002
BmsI GCATC 3 cut(s) 253, 295, 454
BmuI ACTGGG 1 cut(s) 1002
BpiI GAAGAC 1 cut(s) 573
Bpu1102I GCTNAGC 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 846
BsaJI CCNNGG 2 cut(s) 528, 611
Bsc4I CCNNNNNNNGG 2 cut(s) 550, 996
Bse1I ACTGG 3 cut(s) 23, 148, 1008
Bse3DI GCAATG 3 cut(s) 406, 708, 990
BseBI CCWGG 1 cut(s) 653
BseDI CCNNGG 2 cut(s) 528, 611
BseGI GGATG 3 cut(s) 245, 286, 469
BseLI CCNNNNNNNGG 2 cut(s) 550, 996
BseMI GCAATG 3 cut(s) 406, 708, 990
BseNI ACTGG 3 cut(s) 23, 148, 1008
Bsh1236I CGCG 1 cut(s) 589
BshFI GGCC 2 cut(s) 200, 543
BshNI GGYRCC 1 cut(s) 730
BsiHKCI CYCGRG 2 cut(s) 182, 907
BsiSI CCGG 1 cut(s) 843
BslI CCNNNNNNNGG 2 cut(s) 550, 996
BsnI GGCC 2 cut(s) 200, 543
BsoBI CYCGRG 2 cut(s) 182, 907
Bsp1286I GDGCHC 1 cut(s) 908
Bsp143I GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
Bsp1720I GCTNAGC 1 cut(s) 57
BspACI CCGC 4 cut(s) 98, 412, 589, 902
BspANI GGCC 2 cut(s) 200, 543
BspFNI CGCG 1 cut(s) 589
BspLI GGNNCC 3 cut(s) 670, 732, 1001
BspPI GGATC 4 cut(s) 111, 540, 994, 1007
BspT107I GGYRCC 1 cut(s) 730
BsrDI GCAATG 3 cut(s) 406, 708, 990
BsrI ACTGG 3 cut(s) 23, 148, 1008
BssECI CCNNGG 2 cut(s) 528, 611
BssMI GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
BssNI GRCGYC 1 cut(s) 846
BssT1I CCWWGG 2 cut(s) 528, 611
Bst2UI CCWGG 1 cut(s) 653
Bst6I CTCTTC 1 cut(s) 976
BstACI GRCGYC 1 cut(s) 846
BstC8I GCNNGC 1 cut(s) 904
BstDEI CTNAG 2 cut(s) 57, 573
BstF5I GGATG 3 cut(s) 245, 286, 469
BstFNI CGCG 1 cut(s) 589
BstKTI GATC 7 cut(s) 28, 40, 119, 499, 535, 875, 1002
BstMBI GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
BstMWI GCNNNNNNNGC 1 cut(s) 359
BstNI CCWGG 1 cut(s) 653
BstNSI RCATGY 1 cut(s) 916
BstSCI CCNGG 1 cut(s) 651
BstUI CGCG 1 cut(s) 589
BstV2I GAAGAC 1 cut(s) 573
BstX2I RGATCY 6 cut(s) 25, 37, 116, 532, 872, 999
BstYI RGATCY 6 cut(s) 25, 37, 116, 532, 872, 999
BsuI GTATCC 1 cut(s) 610
BsuRI GGCC 2 cut(s) 200, 543
BtsCI GGATG 3 cut(s) 245, 286, 469
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 1 cut(s) 904
CseI GACGC 1 cut(s) 835
CsiI ACCWGGT 1 cut(s) 651
Csp6I GTAC 1 cut(s) 683
CviAII CATG 6 cut(s) 73, 111, 196, 394, 539, 913
CviJI RGCY 9 cut(s) 14, 61, 200, 244, 425, 543, 693, 830, 906
CviKI_1 RGCY 9 cut(s) 14, 61, 200, 244, 425, 543, 693, 830, 906
CviQI GTAC 1 cut(s) 683
DdeI CTNAG 2 cut(s) 57, 573
DpnI GATC 7 cut(s) 27, 39, 118, 498, 534, 874, 1001
DpnII GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
DraI TTTAAA 1 cut(s) 294
EaeI YGGCCR 2 cut(s) 198, 541
Eam1104I CTCTTC 1 cut(s) 976
EarI CTCTTC 1 cut(s) 976
Eco130I CCWWGG 2 cut(s) 528, 611
Eco24I GRGCYC 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 853
Eco88I CYCGRG 2 cut(s) 182, 907
EcoRII CCWGG 1 cut(s) 651
EcoT14I CCWWGG 2 cut(s) 528, 611
EcoT38I GRGCYC 1 cut(s) 908
ErhI CCWWGG 2 cut(s) 528, 611
FaeI CATG 6 cut(s) 76, 114, 199, 397, 542, 916
FatI CATG 6 cut(s) 72, 110, 195, 393, 538, 912
FauI CCCGC 2 cut(s) 582, 895
Fnu4HI GCNGC 1 cut(s) 412
FokI GGATG 3 cut(s) 252, 273, 476
FriOI GRGCYC 1 cut(s) 908
Fsp4HI GCNGC 1 cut(s) 412
FspBI CTAG 2 cut(s) 456, 975
GluI GCNGC 1 cut(s) 412
HaeIII GGCC 2 cut(s) 200, 543
HapII CCGG 1 cut(s) 843
HgaI GACGC 1 cut(s) 835
Hin1I GRCGYC 1 cut(s) 846
Hin1II CATG 6 cut(s) 76, 114, 199, 397, 542, 916
HinfI GANTC 2 cut(s) 254, 659
HpaII CCGG 1 cut(s) 843
Hpy188I TCNGA 6 cut(s) 25, 329, 382, 639, 763, 872
Hpy188III TCNNGA 4 cut(s) 35, 206, 698, 796
HpyAV CCTTC 2 cut(s) 181, 378
HpyCH4IV ACGT 1 cut(s) 221
HpyCH4V TGCA 7 cut(s) 72, 266, 286, 432, 467, 861, 983
HpyF10VI GCNNNNNNNGC 1 cut(s) 359
HpyF3I CTNAG 2 cut(s) 57, 573
HpySE526I ACGT 1 cut(s) 221
Hsp92I GRCGYC 1 cut(s) 846
Hsp92II CATG 6 cut(s) 76, 114, 199, 397, 542, 916
Kzo9I GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
LmnI GCTCC 1 cut(s) 514
LpnPI CCDG 9 cut(s) 4, 129, 330, 400, 638, 651, 655, 665, 856
LweI GCATC 3 cut(s) 253, 295, 454
MabI ACCWGGT 1 cut(s) 651
MaeI CTAG 2 cut(s) 456, 975
MaeII ACGT 1 cut(s) 221
MaeIII GTNAC 3 cut(s) 312, 557, 944
MalI GATC 7 cut(s) 27, 39, 118, 498, 534, 874, 1001
MboI GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
MboII GAAGA 9 cut(s) 20, 222, 527, 578, 641, 804, 908, 944, 963
MfeI CAATTG 3 cut(s) 742, 822, 856
MflI RGATCY 6 cut(s) 25, 37, 116, 532, 872, 999
MhlI GDGCHC 1 cut(s) 908
MlsI TGGCCA 1 cut(s) 200
MluCI AATT 7 cut(s) 335, 484, 518, 742, 772, 822, 856
MluNI TGGCCA 1 cut(s) 200
MmeI TCCRAC 1 cut(s) 662
MnlI CCTC 5 cut(s) 42, 70, 184, 721, 820
Mox20I TGGCCA 1 cut(s) 200
MroXI GAANNNNTTC 1 cut(s) 484
MscI TGGCCA 1 cut(s) 200
MseI TTAA 5 cut(s) 152, 275, 293, 768, 1015
MslI CAYNNNNRTG 1 cut(s) 543
Msp20I TGGCCA 1 cut(s) 200
MspI CCGG 1 cut(s) 843
MspR9I CCNGG 1 cut(s) 653
MunI CAATTG 3 cut(s) 742, 822, 856
MvaI CCWGG 1 cut(s) 653
MvnI CGCG 1 cut(s) 589
MwoI GCNNNNNNNGC 1 cut(s) 359
NdeII GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
NlaIII CATG 6 cut(s) 76, 114, 199, 397, 542, 916
NlaIV GGNNCC 3 cut(s) 670, 732, 1001
NmuCI GTSAC 2 cut(s) 557, 944
NspI RCATGY 1 cut(s) 916
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 2 cut(s) 254, 659
PkrI GCNGC 1 cut(s) 413
PsiI TTATAA 2 cut(s) 341, 965
Psp1406I AACGTT 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 651
PspGI CCWGG 1 cut(s) 651
PspN4I GGNNCC 3 cut(s) 670, 732, 1001
PsrI GAACNNNNNNTAC 2 cut(s) 314, 346
PsuI RGATCY 6 cut(s) 25, 37, 116, 532, 872, 999
RsaI GTAC 1 cut(s) 684
RsaNI GTAC 1 cut(s) 683
RseI CAYNNNNRTG 1 cut(s) 543
SaqAI TTAA 5 cut(s) 152, 275, 293, 768, 1015
SatI GCNGC 1 cut(s) 412
Sau3AI GATC 7 cut(s) 25, 37, 116, 496, 532, 872, 999
ScrFI CCNGG 1 cut(s) 653
SduI GDGCHC 1 cut(s) 908
SetI ASST 7 cut(s) 63, 224, 654, 674, 732, 791, 811
SexAI ACCWGGT 1 cut(s) 651
SfaNI GCATC 3 cut(s) 253, 295, 454
SmiMI CAYNNNNRTG 1 cut(s) 543
SmlI CTYRAG 1 cut(s) 33
SmoI CTYRAG 1 cut(s) 33
Sse9I AATT 7 cut(s) 335, 484, 518, 742, 772, 822, 856
SsiI CCGC 4 cut(s) 98, 412, 589, 902
SspMI CTAG 2 cut(s) 456, 975
StyD4I CCNGG 1 cut(s) 651
StyI CCWWGG 2 cut(s) 528, 611
TaiI ACGT 1 cut(s) 224
TasI AATT 7 cut(s) 335, 484, 518, 742, 772, 822, 856
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 2 cut(s) 254, 659
Tru1I TTAA 5 cut(s) 152, 275, 293, 768, 1015
Tru9I TTAA 5 cut(s) 152, 275, 293, 768, 1015
TscAI CASTG 1 cut(s) 127
TseFI GTSAC 2 cut(s) 557, 944
Tsp45I GTSAC 2 cut(s) 557, 944
TspDTI ATGAA 2 cut(s) 527, 951
TspGWI ACGGA 2 cut(s) 156, 944
TspRI CASTG 1 cut(s) 127
XapI RAATTY 2 cut(s) 335, 772
XceI RCATGY 1 cut(s) 916
XcmI CCANNNNNNNNNTGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 2 cut(s) 456, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.