RchiOBHm_Chr5g0006331

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000399
Physical Location & Seq
Reverse (-)
3942472 .. 3943659
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28746

Sequence Viewer

Length: 1188 bp
ATGGCTGTCGACTGGTCTACGGATATTCCTCGAGATCTTGTTCTCTGTTTCGCCAATCGGTTGATGGTTTCATTGGAGGATTTTATTGCTTTCGGTGCAGTTTGCAAATCATGGAGTTCAGCGGCGATGGAGGTAAAGGAAAACTACCTTGCTAGTATTAGTACTAGTACCAGTACTAGCACTGGATTGAGATTATTAGCATATCAAGTTCCTCTCCTAATGCTACCGATAAGACTTACCCTTCCACGGGGTGTTGCAGGCGGCGACGTGACGGTTGAATTTTACAGTCTCACTGCAAAAGGTAATAAGATTTACAGAAGTAAATTACTAGATGCTAAAGGTAAAATGTGCTATTCTTCTTTAGGATGGCTGGTCACTGTATCTAAAGACTTGGAATTCAAACTGTTACAGCCCTTCAAACATGCCATGAACATTCAACTCCCAGATGGACGAGCTAGTTCGCTACAATATCCCCTTGAAGATAGAGGCAAGTTTGTCTTATCGTCGAGTCCTTCTTGGACATCGGATTACGTAGTCATGTTTCATACTTCTACTACTTTGGCATACTGCAGACCAGGACTAGGTGAAAATTGGACGGAGCTATCATTCCAAAGGGACAATATTAATATTATCAGTGATCTGACTTACTATCGGGGACAATTTTATGTGGTGGACTGTTGTGGCCGTGTTTTAGTCGTTTGTGATATTGACGACCCTAATCGAGCAAAACTGAAGGTTGTTGCTCCAGAGATAAATCAAAAGGAACTACTTGGTACTAGGAGGCACATTAAACAACTGTACTTGGCGGAATGCGCGGGAGCTCTATTGCTTGTTTTTTGTCTCTTCTCCAATAAGTATGAATCCACTACCGCCTGTAGGGTTTTCGAGGTCCCATTTAATAACGGCAAATCATGGAACGACTCTGAGGTCAAGGATTTGGGGAATAGAGCAATCTTCTTGAGTCAAAGTAGTTCTTCCTTCTGTATTGAAGTCACTGATTATTCCGGATGTAAAGCCAATTGCATATACTTCATGAACAATATGTTTGTCTCTTCAGTGCTAAACATTGATATGGGTATTTATAATATGAGCAACGCGTCAATCGACCGAGAATTTGGCAAATCCTTCAATTATGATTTTAAAGATAAGAGTGGATCGCATTTATGGATTCAACCGAGTTTTGAATAG

Protein Analysis

395

Amino Acids

44.43

Weight (kDa)

6.88

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 94 - 362 2.7e-46 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1083
AasI GACNNNNNNGTC 1 cut(s) 926
AccI GTMKAC 2 cut(s) 9, 17
AccII CGCG 2 cut(s) 815, 1097
AccIII TCCGGA 1 cut(s) 1004
AciI CCGC 5 cut(s) 122, 261, 806, 815, 870
AclWI GGATC 1 cut(s) 1162
AcoI YGGCCR 1 cut(s) 682
AcsI RAATTY 3 cut(s) 278, 395, 1112
AcuI CTGAAG 2 cut(s) 752, 1038
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 5 cut(s) 163, 169, 175, 775, 800
AfiI CCNNNNNNNGG 4 cut(s) 246, 247, 581, 876
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 9 cut(s) 278, 400, 418, 437, 479, 989, 1129, 1172, 1184
AhlI ACTAGT 1 cut(s) 164
AjiI CACGTC 1 cut(s) 268
AjnI CCWGG 1 cut(s) 574
AluBI AGCT 3 cut(s) 455, 601, 821
AluI AGCT 3 cut(s) 455, 601, 821
Alw21I GWGCWC 1 cut(s) 823
Alw26I GTCTC 3 cut(s) 293, 845, 1054
AlwI GGATC 1 cut(s) 1162
Ama87I CYCGRG 1 cut(s) 30
Aor13HI TCCGGA 1 cut(s) 1004
AoxI GGCC 1 cut(s) 682
ApoI RAATTY 3 cut(s) 278, 395, 1112
ArsI GACNNNNNNTTYG 2 cut(s) 1097, 1129
AseI ATTAAT 1 cut(s) 624
AspLEI GCGC 1 cut(s) 815
AspS9I GGNCC 1 cut(s) 889
AsuHPI GGTGA 1 cut(s) 596
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 823
Bbv12I GWGCWC 1 cut(s) 823
BccI CCATC 4 cut(s) 58, 121, 360, 440
BceAI ACGGC 2 cut(s) 669, 919
BciT130I CCWGG 1 cut(s) 576
BcoDI GTCTC 3 cut(s) 293, 845, 1054
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 7 cut(s) 153, 165, 177, 329, 456, 581, 777
BfmI CTRYAG 2 cut(s) 568, 874
BglII AGATCT 1 cut(s) 34
BisI GCNGC 2 cut(s) 123, 262
BlsI GCNGC 2 cut(s) 124, 263
BmcAI AGTACT 2 cut(s) 163, 175
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 1 cut(s) 889
BmeT110I CYCGRG 1 cut(s) 30
BmgBI CACGTC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 889
BmiI GGNNCC 1 cut(s) 891
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 1 cut(s) 322
BpmI CTGGAG 1 cut(s) 729
BpuEI CTTGAG 1 cut(s) 979
BsaAI YACGTR 1 cut(s) 532
BsaJI CCNNGG 1 cut(s) 245
BsaWI WCCGGW 1 cut(s) 1004
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 247, 581, 876
Bse1I ACTGG 3 cut(s) 17, 171, 187
BseAI TCCGGA 1 cut(s) 1004
BseBI CCWGG 1 cut(s) 576
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 2 cut(s) 371, 1013
BseLI CCNNNNNNNGG 4 cut(s) 246, 247, 581, 876
BseMII CTCAG 1 cut(s) 915
BseNI ACTGG 3 cut(s) 17, 171, 187
BsgI GTGCAG 1 cut(s) 117
Bsh1236I CGCG 2 cut(s) 815, 1097
Bsh1285I CGRYCG 1 cut(s) 1108
BshFI GGCC 1 cut(s) 684
BsiEI CGRYCG 1 cut(s) 1108
BsiHKAI GWGCWC 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 1 cut(s) 1005
BslFI GGGAC 3 cut(s) 629, 669, 875
BslI CCNNNNNNNGG 4 cut(s) 246, 247, 581, 876
BsmAI GTCTC 3 cut(s) 293, 845, 1054
BsmFI GGGAC 3 cut(s) 629, 669, 875
BsmI GAATGC 1 cut(s) 815
BsnI GGCC 1 cut(s) 684
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 823
Bsp13I TCCGGA 1 cut(s) 1004
Bsp143I GATC 3 cut(s) 34, 637, 1154
BspACI CCGC 5 cut(s) 122, 261, 806, 815, 870
BspANI GGCC 1 cut(s) 684
BspCNI CTCAG 1 cut(s) 916
BspEI TCCGGA 1 cut(s) 1004
BspFNI CGCG 2 cut(s) 815, 1097
BspHI TCATGA 1 cut(s) 1032
BspLI GGNNCC 1 cut(s) 891
BspMAI CTGCAG 1 cut(s) 572
BspPI GGATC 1 cut(s) 1162
BsrI ACTGG 3 cut(s) 17, 171, 187
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 3 cut(s) 34, 637, 1154
Bst2UI CCWGG 1 cut(s) 576
Bst4CI ACNGT 6 cut(s) 274, 287, 379, 405, 677, 798
Bst6I CTCTTC 2 cut(s) 848, 1057
BstBAI YACGTR 1 cut(s) 532
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 1 cut(s) 924
BstDSI CCRYGG 1 cut(s) 245
BstF5I GGATG 2 cut(s) 371, 1013
BstFNI CGCG 2 cut(s) 815, 1097
BstHHI GCGC 1 cut(s) 815
BstKTI GATC 3 cut(s) 37, 640, 1157
BstMAI GTCTC 3 cut(s) 293, 845, 1054
BstMBI GATC 3 cut(s) 34, 637, 1154
BstMCI CGRYCG 1 cut(s) 1108
BstMWI GCNNNNNNNGC 2 cut(s) 95, 812
BstNI CCWGG 1 cut(s) 576
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 2 cut(s) 568, 874
BstSNI TACGTA 1 cut(s) 532
BstUI CGCG 2 cut(s) 815, 1097
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuRI GGCC 1 cut(s) 684
BtgI CCRYGG 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 140
BtrI CACGTC 1 cut(s) 268
BtsCI GGATG 2 cut(s) 371, 1013
BtsI GCAGTG 1 cut(s) 291
BtsIMutI CAGTG 6 cut(s) 180, 291, 375, 640, 993, 1062
Cac8I GCNNGC 1 cut(s) 259
CciI TCATGA 1 cut(s) 1032
CfoI GCGC 1 cut(s) 815
Cfr13I GGNCC 1 cut(s) 889
CseI GACGC 1 cut(s) 1086
Csp6I GTAC 5 cut(s) 162, 168, 174, 774, 799
CviAII CATG 6 cut(s) 111, 422, 427, 538, 912, 1033
CviJI RGCY 8 cut(s) 5, 370, 412, 455, 601, 684, 821, 1016
CviKI_1 RGCY 8 cut(s) 5, 370, 412, 455, 601, 684, 821, 1016
CviQI GTAC 5 cut(s) 162, 168, 174, 774, 799
DdeI CTNAG 1 cut(s) 924
DpnI GATC 3 cut(s) 36, 639, 1156
DpnII GATC 3 cut(s) 34, 637, 1154
DraI TTTAAA 1 cut(s) 1141
DraIII CACNNNGTG 1 cut(s) 251
DrdI GACNNNNNNGTC 1 cut(s) 926
DseDI GACNNNNNNGTC 1 cut(s) 926
EaeI YGGCCR 1 cut(s) 682
Eam1104I CTCTTC 2 cut(s) 848, 1057
EarI CTCTTC 2 cut(s) 848, 1057
EciI GGCGGA 1 cut(s) 821
Ecl136II GAGCTC 1 cut(s) 821
Eco105I TACGTA 1 cut(s) 532
Eco24I GRGCYC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 821
Eco57I CTGAAG 2 cut(s) 752, 1038
Eco88I CYCGRG 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 821
EcoO109I RGGNCCY 1 cut(s) 889
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 574
EcoT38I GRGCYC 1 cut(s) 823
FaeI CATG 6 cut(s) 114, 425, 430, 541, 915, 1036
FalI AAGNNNNNCTT 4 cut(s) 482, 514, 958, 990
FaqI GGGAC 3 cut(s) 629, 669, 875
FatI CATG 6 cut(s) 110, 421, 426, 537, 911, 1032
FauI CCCGC 1 cut(s) 808
FblI GTMKAC 2 cut(s) 9, 17
Fnu4HI GCNGC 2 cut(s) 123, 262
FokI GGATG 2 cut(s) 378, 1020
FriOI GRGCYC 1 cut(s) 823
Fsp4HI GCNGC 2 cut(s) 123, 262
FspBI CTAG 7 cut(s) 153, 165, 177, 329, 456, 581, 777
GlaI GCGC 1 cut(s) 814
GluI GCNGC 2 cut(s) 123, 262
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 1 cut(s) 684
HapII CCGG 1 cut(s) 1005
HgaI GACGC 1 cut(s) 1086
HhaI GCGC 1 cut(s) 815
Hin1II CATG 6 cut(s) 114, 425, 430, 541, 915, 1036
Hin6I GCGC 1 cut(s) 813
HinP1I GCGC 1 cut(s) 813
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 5 cut(s) 508, 860, 920, 961, 1168
HpaII CCGG 1 cut(s) 1005
HphI GGTGA 1 cut(s) 596
Hpy166II GTNNAC 3 cut(s) 10, 18, 673
Hpy188I TCNGA 3 cut(s) 526, 642, 925
Hpy188III TCNNGA 5 cut(s) 32, 746, 958, 1005, 1033
Hpy8I GTNNAC 3 cut(s) 10, 18, 673
Hpy99I CGWCG 2 cut(s) 269, 508
HpyAV CCTTC 6 cut(s) 251, 424, 522, 727, 988, 1135
HpyCH4III ACNGT 6 cut(s) 274, 287, 379, 405, 677, 798
HpyCH4IV ACGT 2 cut(s) 267, 531
HpyCH4V TGCA 6 cut(s) 98, 105, 257, 296, 570, 1023
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 812
HpyF3I CTNAG 1 cut(s) 924
HpySE526I ACGT 2 cut(s) 267, 531
Hsp92II CATG 6 cut(s) 114, 425, 430, 541, 915, 1036
HspAI GCGC 1 cut(s) 813
Kpn2I TCCGGA 1 cut(s) 1004
Kzo9I GATC 3 cut(s) 34, 637, 1154
LmnI GCTCC 3 cut(s) 598, 748, 818
LweI GCATC 1 cut(s) 322
MaeI CTAG 7 cut(s) 153, 165, 177, 329, 456, 581, 777
MaeII ACGT 2 cut(s) 267, 531
MaeIII GTNAC 4 cut(s) 268, 373, 405, 991
MalI GATC 3 cut(s) 36, 639, 1156
MboI GATC 3 cut(s) 34, 637, 1154
MboII GAAGA 6 cut(s) 348, 491, 835, 946, 966, 1044
MfeI CAATTG 1 cut(s) 1018
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 823
MluCI AATT 8 cut(s) 278, 323, 395, 589, 659, 1018, 1112, 1129
MluI ACGCGT 1 cut(s) 1095
MlyI GAGTC 3 cut(s) 517, 914, 970
MnlI CCTC 8 cut(s) 39, 70, 124, 222, 479, 774, 880, 919
MroI TCCGGA 1 cut(s) 1004
MseI TTAA 4 cut(s) 624, 789, 897, 1140
MslI CAYNNNNRTG 1 cut(s) 1070
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 1 cut(s) 1005
MspR9I CCNGG 1 cut(s) 576
MunI CAATTG 1 cut(s) 1018
Mva1269I GAATGC 1 cut(s) 815
MvaI CCWGG 1 cut(s) 576
MvnI CGCG 2 cut(s) 815, 1097
MwoI GCNNNNNNNGC 2 cut(s) 95, 812
NdeII GATC 3 cut(s) 34, 637, 1154
NlaIII CATG 6 cut(s) 114, 425, 430, 541, 915, 1036
NlaIV GGNNCC 1 cut(s) 891
NmuCI GTSAC 3 cut(s) 268, 373, 991
NspI RCATGY 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 1 cut(s) 1032
PcsI WCGNNNNNNNCGW 1 cut(s) 1101
PctI GAATGC 1 cut(s) 815
PfeI GAWTC 2 cut(s) 860, 1168
PkrI GCNGC 2 cut(s) 124, 263
PleI GAGTC 3 cut(s) 516, 914, 969
PpsI GAGTC 3 cut(s) 516, 914, 969
Ppu21I YACGTR 1 cut(s) 532
PpuMI RGGWCCY 1 cut(s) 889
PshBI ATTAAT 1 cut(s) 624
PsiI TTATAA 1 cut(s) 1083
Psp124BI GAGCTC 1 cut(s) 823
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 1 cut(s) 574
PspGI CCWGG 1 cut(s) 574
PspN4I GGNNCC 1 cut(s) 891
PspPI GGNCC 1 cut(s) 889
PspPPI RGGWCCY 1 cut(s) 889
PstI CTGCAG 1 cut(s) 572
PsuI RGATCY 1 cut(s) 34
RsaI GTAC 5 cut(s) 163, 169, 175, 775, 800
RsaNI GTAC 5 cut(s) 162, 168, 174, 774, 799
RseI CAYNNNNRTG 1 cut(s) 1070
SacI GAGCTC 1 cut(s) 823
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 4 cut(s) 624, 789, 897, 1140
SatI GCNGC 2 cut(s) 123, 262
Sau3AI GATC 3 cut(s) 34, 637, 1154
Sau96I GGNCC 1 cut(s) 889
ScaI AGTACT 2 cut(s) 163, 175
SchI GAGTC 3 cut(s) 517, 914, 970
ScrFI CCNGG 1 cut(s) 576
SduI GDGCHC 1 cut(s) 823
SfaNI GCATC 1 cut(s) 322
SfcI CTRYAG 2 cut(s) 568, 874
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 889
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 2 cut(s) 30, 958
SmoI CTYRAG 2 cut(s) 30, 958
SnaBI TACGTA 1 cut(s) 532
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 8 cut(s) 278, 323, 395, 589, 659, 1018, 1112, 1129
SsiI CCGC 5 cut(s) 122, 261, 806, 815, 870
SspI AATATT 2 cut(s) 622, 628
SspMI CTAG 7 cut(s) 153, 165, 177, 329, 456, 581, 777
SstI GAGCTC 1 cut(s) 823
StyD4I CCNGG 1 cut(s) 574
TaaI ACNGT 6 cut(s) 274, 287, 379, 405, 677, 798
TaiI ACGT 2 cut(s) 270, 534
TaqI TCGA 6 cut(s) 9, 31, 506, 721, 885, 1104
TaqII GACCGA 1 cut(s) 1122
TasI AATT 8 cut(s) 278, 323, 395, 589, 659, 1018, 1112, 1129
TatI WGTACW 3 cut(s) 161, 173, 798
TauI GCSGC 2 cut(s) 125, 264
TfiI GAWTC 2 cut(s) 860, 1168
Tru1I TTAA 4 cut(s) 624, 789, 897, 1140
Tru9I TTAA 4 cut(s) 624, 789, 897, 1140
TscAI CASTG 6 cut(s) 187, 298, 382, 640, 1000, 1062
TseFI GTSAC 3 cut(s) 268, 373, 991
Tsp45I GTSAC 3 cut(s) 268, 373, 991
TspDTI ATGAA 6 cut(s) 60, 443, 533, 873, 1021, 1049
TspGWI ACGGA 2 cut(s) 35, 611
TspRI CASTG 6 cut(s) 187, 298, 382, 640, 1000, 1062
VpaK11BI GGWCC 1 cut(s) 889
VspI ATTAAT 1 cut(s) 624
XapI RAATTY 3 cut(s) 278, 395, 1112
XceI RCATGY 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 9, 17
XspI CTAG 7 cut(s) 153, 165, 177, 329, 456, 581, 777
ZrmI AGTACT 2 cut(s) 163, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.