Rroxscaffold_7G00181120

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000399
Physical Location & Seq
Reverse (-)
20344099 .. 20345519
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00181120.1

Sequence Viewer

Length: 1074 bp
ATGGCTACCGGGTGGTGCGATTCTCTTCCGAATTACCTTGTCTCTCTGCGCGAAAGGCTGGCAGTTTTCTGGAACAAGTTTATCACCGCAGTGCAGAAATCATGGGGACCATTCGGCGCTAATCAAGTTCCTCTTCTTATGCTTCCGAAGAGGATTGATGCCTCCATGGTTCAGTTTTACACCGTTGCAAACGGTGAGATCTACAACTCCAATCTCCCAGAAACCAGTGGTAAGGTCTGCTTTGCTTCCCTGGGATGGCTGCTTACTGTAACTACGGATCAAGGGGAATTGAAATTTAATATGCTCCACCCATCAAATCATCATGCCAAAATTCAACTTCCTGGTTTGCTGCACTATCCGCTCGAGAATATTTGGAAGTTTGCTTTGTCGTCCAATCCTCGTTCCACATTGGATTATATAGTCATGTTTCAGACAGCTACTAGTTTGGGATTTTGCAGACCAGGACTGGGCGGCCAAGGTTGGAAACATCTCAGATTTGAGGGCCACAATTTCATTAGGGATCTCACACACTATCAAGGAAGTTTCTACGTTGTGGACCAATCTGGCCGTGTTTCTGTTGTTTGTGAAATTGAAGACCCTAAGCGAGCACATTTGAGTGTTGTTGCTCCAGAGATTCCGAGTGAGGAGCAACTTCTAGGTCCTATGAAGGGCATTAAACAGCTATATCTGGTGGAATCTGCAGGGTCCCTATTGGTGGTTTTGTGTACTTCCTACCATAATCATTATGAATCAACTATTGGCTGCAGGGTTTTCAAGGTCCCATTCAGTAATGGCAATTCATGGTCCGACTCAGAGGTAAAGGATTTGGGTAATAGAGCCCTCTTCTTGAGTAACAGCAGTTCTTCATTCTCTATTGAGGGCTCAGGCAATTCCGGATGCAAGCCCAATTGCATATATTTCATGAACAGCAAAAGGGTCTCTCCATTCGTTGACTTAGATGTTGGTATTTTTAATATGGAGGATGGGACAATTGATCGAGACTTCAGCAATTCCTTCAATACCGGGTTTGAAGAAATCAGAGAATCCCATCTGTGGATTCAACCGAGTTTCTGA

Protein Analysis

357

Amino Acids

40.02

Weight (kDa)

6.26

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 59 - 325 6.2e-44 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 361
AccII CGCG 1 cut(s) 51
AccIII TCCGGA 1 cut(s) 893
AciI CCGC 3 cut(s) 87, 359, 471
AclWI GGATC 2 cut(s) 285, 528
AcoI YGGCCR 2 cut(s) 472, 565
AcsI RAATTY 2 cut(s) 293, 330
AcuI CTGAAG 1 cut(s) 988
AfaI GTAC 1 cut(s) 727
AfiI CCNNNNNNNGG 5 cut(s) 255, 467, 668, 715, 1053
AgsI TTSAA 7 cut(s) 292, 335, 593, 775, 1018, 1031, 1061
AhlI ACTAGT 1 cut(s) 440
AjnI CCWGG 3 cut(s) 249, 340, 460
AjuI GAANNNNNNNTTGG 2 cut(s) 741, 773
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 2 cut(s) 437, 682
AluI AGCT 2 cut(s) 437, 682
Alw21I GWGCWC 1 cut(s) 610
Alw26I GTCTC 3 cut(s) 46, 943, 993
AlwI GGATC 2 cut(s) 285, 528
Ama87I CYCGRG 1 cut(s) 362
Aor13HI TCCGGA 1 cut(s) 893
AoxI GGCC 3 cut(s) 472, 502, 565
ApeKI GCWGC 3 cut(s) 259, 349, 762
ApoI RAATTY 2 cut(s) 293, 330
AspLEI GCGC 2 cut(s) 51, 119
AspS9I GGNCC 7 cut(s) 107, 502, 556, 659, 705, 778, 804
AsuC2I CCSGG 2 cut(s) 10, 1024
AsuHPI GGTGA 2 cut(s) 76, 206
AvaI CYCGRG 1 cut(s) 362
AvaII GGWCC 6 cut(s) 107, 556, 659, 705, 778, 804
BanII GRGCYC 2 cut(s) 841, 884
BbsI GAAGAC 1 cut(s) 600
Bbv12I GWGCWC 1 cut(s) 610
BbvI GCAGC 3 cut(s) 246, 336, 749
BccI CCATC 4 cut(s) 249, 319, 977, 1056
BceAI ACGGC 1 cut(s) 552
BciT130I CCWGG 3 cut(s) 251, 342, 462
BcnI CCSGG 2 cut(s) 10, 1024
BcoDI GTCTC 3 cut(s) 46, 943, 993
BcuI ACTAGT 1 cut(s) 440
BfaI CTAG 2 cut(s) 441, 656
BfmI CTRYAG 2 cut(s) 699, 763
BfoI RGCGCY 1 cut(s) 120
BglII AGATCT 1 cut(s) 198
BisI GCNGC 4 cut(s) 260, 350, 472, 763
BlsI GCNGC 4 cut(s) 261, 351, 473, 764
Bme1390I CCNGG 5 cut(s) 10, 251, 342, 462, 1024
Bme18I GGWCC 6 cut(s) 107, 556, 659, 705, 778, 804
BmeT110I CYCGRG 1 cut(s) 362
BmgT120I GGNCC 7 cut(s) 107, 502, 556, 659, 705, 778, 804
BmiI GGNNCC 4 cut(s) 108, 706, 707, 780
BmrFI CCNGG 5 cut(s) 10, 251, 342, 462, 1024
BmrI ACTGGG 1 cut(s) 476
BmsI GCATC 2 cut(s) 148, 887
BmuI ACTGGG 1 cut(s) 476
BpiI GAAGAC 1 cut(s) 600
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 2 cut(s) 600, 883
BpuEI CTTGAG 1 cut(s) 868
BpuMI CCSGG 2 cut(s) 10, 1024
BsaI GGTCTC 1 cut(s) 943
BsaJI CCNNGG 4 cut(s) 165, 249, 250, 475
BsaWI WCCGGW 1 cut(s) 893
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 467, 668, 715, 1053
Bse1I ACTGG 2 cut(s) 225, 471
BseAI TCCGGA 1 cut(s) 893
BseBI CCWGG 3 cut(s) 251, 342, 462
BseDI CCNNGG 4 cut(s) 165, 249, 250, 475
BseGI GGATG 3 cut(s) 260, 902, 988
BseLI CCNNNNNNNGG 5 cut(s) 255, 467, 668, 715, 1053
BseMII CTCAG 3 cut(s) 505, 825, 897
BseNI ACTGG 2 cut(s) 225, 471
BseRI GAGGAG 1 cut(s) 659
BseXI GCAGC 3 cut(s) 246, 336, 749
BsgI GTGCAG 2 cut(s) 113, 335
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 3 cut(s) 474, 504, 567
BsiHKAI GWGCWC 1 cut(s) 610
BsiHKCI CYCGRG 1 cut(s) 362
BsiSI CCGG 3 cut(s) 9, 894, 1023
BslFI GGGAC 4 cut(s) 120, 691, 764, 1000
BslI CCNNNNNNNGG 5 cut(s) 255, 467, 668, 715, 1053
BsmAI GTCTC 3 cut(s) 46, 943, 993
BsmFI GGGAC 4 cut(s) 120, 691, 764, 1000
BsnI GGCC 3 cut(s) 474, 504, 567
Bso31I GGTCTC 1 cut(s) 943
BsoBI CYCGRG 1 cut(s) 362
Bsp1286I GDGCHC 3 cut(s) 610, 841, 884
Bsp13I TCCGGA 1 cut(s) 893
Bsp143I GATC 4 cut(s) 198, 277, 520, 994
Bsp19I CCATGG 1 cut(s) 165
BspACI CCGC 3 cut(s) 87, 359, 471
BspANI GGCC 3 cut(s) 474, 504, 567
BspCNI CTCAG 3 cut(s) 504, 824, 896
BspEI TCCGGA 1 cut(s) 893
BspFNI CGCG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 921
BspLI GGNNCC 4 cut(s) 108, 706, 707, 780
BspMAI CTGCAG 2 cut(s) 703, 767
BspPI GGATC 2 cut(s) 285, 528
BspTNI GGTCTC 1 cut(s) 943
BsrBI CCGCTC 1 cut(s) 361
BsrI ACTGG 2 cut(s) 225, 471
BssECI CCNNGG 4 cut(s) 165, 249, 250, 475
BssMI GATC 4 cut(s) 198, 277, 520, 994
BssT1I CCWWGG 2 cut(s) 165, 475
Bst2UI CCWGG 3 cut(s) 251, 342, 462
Bst4CI ACNGT 3 cut(s) 184, 194, 268
Bst6I CTCTTC 4 cut(s) 30, 138, 143, 848
BstC8I GCNNGC 3 cut(s) 60, 606, 902
BstDEI CTNAG 5 cut(s) 491, 600, 811, 883, 955
BstDSI CCRYGG 1 cut(s) 165
BstF5I GGATG 3 cut(s) 260, 902, 988
BstFNI CGCG 1 cut(s) 51
BstH2I RGCGCY 1 cut(s) 120
BstHHI GCGC 2 cut(s) 51, 119
BstKTI GATC 4 cut(s) 201, 280, 523, 997
BstMAI GTCTC 3 cut(s) 46, 943, 993
BstMBI GATC 4 cut(s) 198, 277, 520, 994
BstMWI GCNNNNNNNGC 2 cut(s) 55, 358
BstNI CCWGG 3 cut(s) 251, 342, 462
BstSCI CCNGG 5 cut(s) 8, 249, 340, 460, 1022
BstSFI CTRYAG 2 cut(s) 699, 763
BstUI CGCG 1 cut(s) 51
BstV1I GCAGC 3 cut(s) 246, 336, 749
BstV2I GAAGAC 1 cut(s) 600
BstX2I RGATCY 2 cut(s) 198, 520
BstYI RGATCY 2 cut(s) 198, 520
BsuRI GGCC 3 cut(s) 474, 504, 567
BtgI CCRYGG 1 cut(s) 165
BtsCI GGATG 3 cut(s) 260, 902, 988
BtsI GCAGTG 1 cut(s) 96
BtsIMutI CAGTG 2 cut(s) 96, 232
Cac8I GCNNGC 3 cut(s) 60, 606, 902
CciI TCATGA 1 cut(s) 921
CfoI GCGC 2 cut(s) 51, 119
Cfr13I GGNCC 7 cut(s) 107, 502, 556, 659, 705, 778, 804
Csp6I GTAC 1 cut(s) 726
CviAII CATG 6 cut(s) 102, 166, 323, 424, 801, 922
CviQI GTAC 1 cut(s) 726
DdeI CTNAG 5 cut(s) 491, 600, 811, 883, 955
DpnI GATC 4 cut(s) 200, 279, 522, 996
DpnII GATC 4 cut(s) 198, 277, 520, 994
EaeI YGGCCR 2 cut(s) 472, 565
Eam1104I CTCTTC 4 cut(s) 30, 138, 143, 848
EarI CTCTTC 4 cut(s) 30, 138, 143, 848
Eco130I CCWWGG 2 cut(s) 165, 475
Eco24I GRGCYC 2 cut(s) 841, 884
Eco31I GGTCTC 1 cut(s) 943
Eco47I GGWCC 6 cut(s) 107, 556, 659, 705, 778, 804
Eco57I CTGAAG 1 cut(s) 988
Eco88I CYCGRG 1 cut(s) 362
EcoO109I RGGNCCY 3 cut(s) 659, 705, 778
EcoRII CCWGG 3 cut(s) 249, 340, 460
EcoT14I CCWWGG 2 cut(s) 165, 475
EcoT38I GRGCYC 2 cut(s) 841, 884
ErhI CCWWGG 2 cut(s) 165, 475
FaeI CATG 6 cut(s) 105, 169, 326, 427, 804, 925
FalI AAGNNNNNCTT 4 cut(s) 117, 149, 224, 256
FaqI GGGAC 4 cut(s) 120, 691, 764, 1000
FatI CATG 6 cut(s) 101, 165, 322, 423, 800, 921
Fnu4HI GCNGC 4 cut(s) 260, 350, 472, 763
FokI GGATG 3 cut(s) 267, 909, 995
FriOI GRGCYC 2 cut(s) 841, 884
Fsp4HI GCNGC 4 cut(s) 260, 350, 472, 763
FspBI CTAG 2 cut(s) 441, 656
GlaI GCGC 2 cut(s) 50, 118
GluI GCNGC 4 cut(s) 260, 350, 472, 763
GsuI CTGGAG 1 cut(s) 612
HaeII RGCGCY 1 cut(s) 120
HaeIII GGCC 3 cut(s) 474, 504, 567
HapII CCGG 3 cut(s) 9, 894, 1023
HhaI GCGC 2 cut(s) 51, 119
Hin1II CATG 6 cut(s) 105, 169, 326, 427, 804, 925
Hin6I GCGC 2 cut(s) 49, 117
HinP1I GCGC 2 cut(s) 49, 117
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 7 cut(s) 20, 634, 695, 749, 809, 1043, 1057
HpaII CCGG 3 cut(s) 9, 894, 1023
HphI GGTGA 2 cut(s) 76, 206
Hpy166II GTNNAC 3 cut(s) 556, 726, 952
Hpy188I TCNGA 9 cut(s) 30, 147, 432, 494, 639, 808, 814, 1040, 1073
Hpy188III TCNNGA 7 cut(s) 70, 364, 629, 847, 894, 922, 998
Hpy8I GTNNAC 3 cut(s) 556, 726, 952
HpyAV CCTTC 2 cut(s) 661, 1024
HpyCH4III ACNGT 3 cut(s) 184, 194, 268
HpyCH4IV ACGT 1 cut(s) 549
HpyCH4V TGCA 8 cut(s) 94, 188, 352, 456, 701, 765, 900, 912
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 358
HpyF3I CTNAG 5 cut(s) 491, 600, 811, 883, 955
HpySE526I ACGT 1 cut(s) 549
Hsp92II CATG 6 cut(s) 105, 169, 326, 427, 804, 925
HspAI GCGC 2 cut(s) 49, 117
KflI GGGWCCC 1 cut(s) 705
Kpn2I TCCGGA 1 cut(s) 893
Kzo9I GATC 4 cut(s) 198, 277, 520, 994
LmnI GCTCC 3 cut(s) 309, 631, 646
Lsp1109I GCAGC 3 cut(s) 246, 336, 749
LweI GCATC 2 cut(s) 148, 887
MaeI CTAG 2 cut(s) 441, 656
MaeII ACGT 1 cut(s) 549
MaeIII GTNAC 2 cut(s) 268, 851
MalI GATC 4 cut(s) 200, 279, 522, 996
MbiI CCGCTC 1 cut(s) 361
MboI GATC 4 cut(s) 198, 277, 520, 994
MboII GAAGA 7 cut(s) 17, 125, 160, 605, 835, 855, 1043
MfeI CAATTG 2 cut(s) 907, 990
MflI RGATCY 2 cut(s) 198, 520
MhlI GDGCHC 3 cut(s) 610, 841, 884
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 2 cut(s) 461, 831
MroI TCCGGA 1 cut(s) 893
MseI TTAA 3 cut(s) 297, 675, 972
MslI CAYNNNNRTG 2 cut(s) 89, 615
MspI CCGG 3 cut(s) 9, 894, 1023
MspR9I CCNGG 5 cut(s) 10, 251, 342, 462, 1024
MunI CAATTG 2 cut(s) 907, 990
MvaI CCWGG 3 cut(s) 251, 342, 462
MvnI CGCG 1 cut(s) 51
MwoI GCNNNNNNNGC 2 cut(s) 55, 358
NciI CCSGG 2 cut(s) 10, 1024
NcoI CCATGG 1 cut(s) 165
NdeII GATC 4 cut(s) 198, 277, 520, 994
NlaIII CATG 6 cut(s) 105, 169, 326, 427, 804, 925
NlaIV GGNNCC 4 cut(s) 108, 706, 707, 780
OliI CACNNNNGTG 1 cut(s) 89
PaeR7I CTCGAG 1 cut(s) 362
PagI TCATGA 1 cut(s) 921
PasI CCCWGGG 1 cut(s) 250
PfeI GAWTC 6 cut(s) 20, 634, 695, 749, 1043, 1057
PkrI GCNGC 4 cut(s) 261, 351, 473, 764
PleI GAGTC 1 cut(s) 803
PpsI GAGTC 1 cut(s) 803
PpuMI RGGWCCY 3 cut(s) 659, 705, 778
Psp5II RGGWCCY 3 cut(s) 659, 705, 778
Psp6I CCWGG 3 cut(s) 249, 340, 460
PspGI CCWGG 3 cut(s) 249, 340, 460
PspN4I GGNNCC 4 cut(s) 108, 706, 707, 780
PspPI GGNCC 7 cut(s) 107, 502, 556, 659, 705, 778, 804
PspPPI RGGWCCY 3 cut(s) 659, 705, 778
PsrI GAACNNNNNNTAC 2 cut(s) 844, 876
PstI CTGCAG 2 cut(s) 703, 767
PsuI RGATCY 2 cut(s) 198, 520
RsaI GTAC 1 cut(s) 727
RsaNI GTAC 1 cut(s) 726
RseI CAYNNNNRTG 2 cut(s) 89, 615
SaqAI TTAA 3 cut(s) 297, 675, 972
SatI GCNGC 4 cut(s) 260, 350, 472, 763
Sau3AI GATC 4 cut(s) 198, 277, 520, 994
Sau96I GGNCC 7 cut(s) 107, 502, 556, 659, 705, 778, 804
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 5 cut(s) 10, 251, 342, 462, 1024
SduI GDGCHC 3 cut(s) 610, 841, 884
SetI ASST 9 cut(s) 39, 237, 439, 481, 552, 661, 684, 780, 819
SfaNI GCATC 2 cut(s) 148, 887
SfcI CTRYAG 2 cut(s) 699, 763
Sfr274I CTCGAG 1 cut(s) 362
SinI GGWCC 6 cut(s) 107, 556, 659, 705, 778, 804
SlaI CTCGAG 1 cut(s) 362
SmiMI CAYNNNNRTG 2 cut(s) 89, 615
SmlI CTYRAG 2 cut(s) 362, 847
SmoI CTYRAG 2 cut(s) 362, 847
SpeI ACTAGT 1 cut(s) 440
SsiI CCGC 3 cut(s) 87, 359, 471
SspI AATATT 1 cut(s) 370
SspMI CTAG 2 cut(s) 441, 656
StyD4I CCNGG 5 cut(s) 8, 249, 340, 460, 1022
StyI CCWWGG 2 cut(s) 165, 475
TaaI ACNGT 3 cut(s) 184, 194, 268
TaiI ACGT 1 cut(s) 552
TaqI TCGA 2 cut(s) 363, 997
TatI WGTACW 1 cut(s) 725
TauI GCSGC 1 cut(s) 474
TfiI GAWTC 6 cut(s) 20, 634, 695, 749, 1043, 1057
Tru1I TTAA 3 cut(s) 297, 675, 972
Tru9I TTAA 3 cut(s) 297, 675, 972
TscAI CASTG 2 cut(s) 96, 232
TseI GCWGC 3 cut(s) 259, 349, 762
TspDTI ATGAA 7 cut(s) 502, 680, 762, 789, 855, 910, 938
TspGWI ACGGA 1 cut(s) 290
TspRI CASTG 2 cut(s) 96, 232
VpaK11BI GGWCC 6 cut(s) 107, 556, 659, 705, 778, 804
XapI RAATTY 2 cut(s) 293, 330
XhoI CTCGAG 1 cut(s) 362
XspI CTAG 2 cut(s) 441, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.