Rw6G031990

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000399
Physical Location & Seq
Forward (+)
56098674 .. 56099744
1071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031990.1

Sequence Viewer

Length: 957 bp
ATGGCTGTCGACTGGTCTACGGATATTCCTCGAGATCTTGTTCTCTGTTTCGCCAATCGGTTGATGGTTTCATTGGAGGATTTTATTGCTTTCGGTGCAGTTTGCAAATCATGGAGTTCAGCGGCGATGGAAGTAAAGGAAAACTACCTTGCTAGTATTAGTACTAGTACCAGTACTAGCACTGGATTGAGATTATTAGCATATCAAGTTCCTCTCCTAATGCTACCGGTAAGACTTACCCTTCCACGGGGTGTTGCAGGCGGCGACATGCTAAAGGTAAAATGTGCTATTCTGGTCACTGTATCTAAAGACTTGGAATTCAAACTGTTACATCCCTTCAAACATGCCATGAACATTCAACTCCCAGATGGACGAACCAGTTCGCTACAATATCCCCTTGAAGATATAGGCAAGTTTGTCTTATCGTCGAGTCCTTCTTGGACATCGGATTACGTAGTCATGTTTCATACTTCTACTACTTTGGCATACTGCAGACCAGGACTAGGTGAAAATTGGACGGAGCTATCATTCCAAACGGCCAATATTAATATAATCAGTGATCTGACTTACTATCGGGGACAATTTTATGTGGTGGACTGTTGTGGCCGTGTTTTAGTCGTTTGTGATATTGACGACCCTAATCGAGCAAAACTGAAGGTTGTTGCTCCAGAGATAAATCAAAAGGAACTATTTGGTACTAGGAGGCACATTAAACAACTGTACTTGGCGGAATGCGCGGGAGCTCTATTGCTTGTTTTTTTGTCCCATTTAATCGGCAAATCATGGAACGACTCTGAGGTCAAGGATTTGGGGAATAGAGCAATCTTCTTGAGTCAAAGTAGTTCTTCCTTCTGTATTGAAGTCACTGATTATTCCGGATGTAAAGCCAATTGCATATACTTCATGAACAATATGTTAGTCTCTTCAGTGCTAAACATTGATATGGGTATTTATTAA

Protein Analysis

318

Amino Acids

35.4

Weight (kDa)

6.22

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 96 - 318 1.5e-34 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 797
AccI GTMKAC 2 cut(s) 9, 17
AccII CGCG 1 cut(s) 737
AccIII TCCGGA 1 cut(s) 875
AciI CCGC 4 cut(s) 122, 261, 728, 737
AcoI YGGCCR 2 cut(s) 537, 604
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 2 cut(s) 674, 909
AdeI CACNNNGTG 1 cut(s) 251
AfaI GTAC 5 cut(s) 163, 169, 175, 697, 722
AfiI CCNNNNNNNGG 3 cut(s) 246, 247, 503
AgeI ACCGGT 1 cut(s) 226
AgsI TTSAA 5 cut(s) 322, 340, 359, 401, 860
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 2 cut(s) 523, 743
AluI AGCT 2 cut(s) 523, 743
Alw21I GWGCWC 1 cut(s) 745
Alw26I GTCTC 1 cut(s) 925
Ama87I CYCGRG 1 cut(s) 30
Aor13HI TCCGGA 1 cut(s) 875
AoxI GGCC 2 cut(s) 537, 604
ApoI RAATTY 1 cut(s) 317
AseI ATTAAT 1 cut(s) 546
AsiGI ACCGGT 1 cut(s) 226
Asp700I GAANNNNTTC 1 cut(s) 379
AspLEI GCGC 1 cut(s) 737
AsuHPI GGTGA 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 30
BanII GRGCYC 1 cut(s) 745
Bbv12I GWGCWC 1 cut(s) 745
BccI CCATC 3 cut(s) 58, 121, 362
BceAI ACGGC 2 cut(s) 552, 591
BciT130I CCWGG 1 cut(s) 498
BcoDI GTCTC 1 cut(s) 925
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 5 cut(s) 153, 165, 177, 503, 699
BfmI CTRYAG 1 cut(s) 490
BglII AGATCT 1 cut(s) 34
BisI GCNGC 2 cut(s) 123, 262
BlsI GCNGC 2 cut(s) 124, 263
BmcAI AGTACT 2 cut(s) 163, 175
Bme1390I CCNGG 1 cut(s) 498
BmeT110I CYCGRG 1 cut(s) 30
BmrFI CCNGG 1 cut(s) 498
BpmI CTGGAG 1 cut(s) 651
BpuEI CTTGAG 1 cut(s) 850
BsaAI YACGTR 1 cut(s) 454
BsaJI CCNNGG 1 cut(s) 245
BsaWI WCCGGW 2 cut(s) 226, 875
Bsc4I CCNNNNNNNGG 3 cut(s) 246, 247, 503
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 4 cut(s) 17, 171, 187, 378
BseAI TCCGGA 1 cut(s) 875
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 245
BseGI GGATG 2 cut(s) 331, 884
BseLI CCNNNNNNNGG 3 cut(s) 246, 247, 503
BseMII CTCAG 1 cut(s) 786
BseNI ACTGG 4 cut(s) 17, 171, 187, 378
BsgI GTGCAG 1 cut(s) 117
Bsh1236I CGCG 1 cut(s) 737
BshFI GGCC 2 cut(s) 539, 606
BshTI ACCGGT 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 745
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 2 cut(s) 227, 876
BslFI GGGAC 2 cut(s) 591, 748
BslI CCNNNNNNNGG 3 cut(s) 246, 247, 503
BsmAI GTCTC 1 cut(s) 925
BsmFI GGGAC 2 cut(s) 591, 748
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 2 cut(s) 539, 606
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 745
Bsp13I TCCGGA 1 cut(s) 875
Bsp143I GATC 2 cut(s) 34, 559
BspACI CCGC 4 cut(s) 122, 261, 728, 737
BspANI GGCC 2 cut(s) 539, 606
BspCNI CTCAG 1 cut(s) 787
BspEI TCCGGA 1 cut(s) 875
BspFNI CGCG 1 cut(s) 737
BspHI TCATGA 1 cut(s) 903
BspMAI CTGCAG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 4 cut(s) 17, 171, 187, 378
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 1 cut(s) 245
BssMI GATC 2 cut(s) 34, 559
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 4 cut(s) 301, 327, 599, 720
Bst6I CTCTTC 1 cut(s) 928
BstBAI YACGTR 1 cut(s) 454
BstC8I GCNNGC 1 cut(s) 259
BstDEI CTNAG 1 cut(s) 795
BstDSI CCRYGG 1 cut(s) 245
BstF5I GGATG 2 cut(s) 331, 884
BstFNI CGCG 1 cut(s) 737
BstHHI GCGC 1 cut(s) 737
BstKTI GATC 2 cut(s) 37, 562
BstMAI GTCTC 1 cut(s) 925
BstMBI GATC 2 cut(s) 34, 559
BstMWI GCNNNNNNNGC 2 cut(s) 95, 734
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 2 cut(s) 271, 347
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 490
BstSNI TACGTA 1 cut(s) 454
BstUI CGCG 1 cut(s) 737
BstX2I RGATCY 1 cut(s) 34
BstYI RGATCY 1 cut(s) 34
BsuRI GGCC 2 cut(s) 539, 606
BtgI CCRYGG 1 cut(s) 245
BtgZI GCGATG 1 cut(s) 140
BtsCI GGATG 2 cut(s) 331, 884
BtsIMutI CAGTG 5 cut(s) 180, 297, 562, 864, 933
Cac8I GCNNGC 1 cut(s) 259
CciI TCATGA 1 cut(s) 903
CfoI GCGC 1 cut(s) 737
Cfr10I RCCGGY 1 cut(s) 226
Csp6I GTAC 5 cut(s) 162, 168, 174, 696, 721
CspAI ACCGGT 1 cut(s) 226
CviAII CATG 7 cut(s) 111, 268, 344, 349, 460, 783, 904
CviJI RGCY 6 cut(s) 5, 523, 539, 606, 743, 887
CviKI_1 RGCY 6 cut(s) 5, 523, 539, 606, 743, 887
CviQI GTAC 5 cut(s) 162, 168, 174, 696, 721
DdeI CTNAG 1 cut(s) 795
DpnI GATC 2 cut(s) 36, 561
DpnII GATC 2 cut(s) 34, 559
DraIII CACNNNGTG 1 cut(s) 251
DrdI GACNNNNNNGTC 1 cut(s) 797
DseDI GACNNNNNNGTC 1 cut(s) 797
EaeI YGGCCR 2 cut(s) 537, 604
Eam1104I CTCTTC 1 cut(s) 928
EarI CTCTTC 1 cut(s) 928
EciI GGCGGA 1 cut(s) 743
Ecl136II GAGCTC 1 cut(s) 743
Eco105I TACGTA 1 cut(s) 454
Eco24I GRGCYC 1 cut(s) 745
Eco53kI GAGCTC 1 cut(s) 743
Eco57I CTGAAG 2 cut(s) 674, 909
Eco88I CYCGRG 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 743
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 496
EcoT38I GRGCYC 1 cut(s) 745
FaeI CATG 7 cut(s) 114, 271, 347, 352, 463, 786, 907
FalI AAGNNNNNCTT 4 cut(s) 404, 436, 829, 861
FaqI GGGAC 2 cut(s) 591, 748
FatI CATG 7 cut(s) 110, 267, 343, 348, 459, 782, 903
FauI CCCGC 1 cut(s) 730
FblI GTMKAC 2 cut(s) 9, 17
Fnu4HI GCNGC 2 cut(s) 123, 262
FokI GGATG 2 cut(s) 318, 891
FriOI GRGCYC 1 cut(s) 745
Fsp4HI GCNGC 2 cut(s) 123, 262
FspBI CTAG 5 cut(s) 153, 165, 177, 503, 699
GlaI GCGC 1 cut(s) 736
GluI GCNGC 2 cut(s) 123, 262
GsuI CTGGAG 1 cut(s) 651
HaeIII GGCC 2 cut(s) 539, 606
HapII CCGG 2 cut(s) 227, 876
HhaI GCGC 1 cut(s) 737
Hin1II CATG 7 cut(s) 114, 271, 347, 352, 463, 786, 907
Hin6I GCGC 1 cut(s) 735
HinP1I GCGC 1 cut(s) 735
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 3 cut(s) 430, 791, 832
HpaII CCGG 2 cut(s) 227, 876
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 3 cut(s) 10, 18, 595
Hpy188I TCNGA 3 cut(s) 448, 564, 796
Hpy188III TCNNGA 5 cut(s) 32, 668, 829, 876, 904
Hpy8I GTNNAC 3 cut(s) 10, 18, 595
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 5 cut(s) 251, 346, 444, 649, 859
HpyCH4III ACNGT 4 cut(s) 301, 327, 599, 720
HpyCH4IV ACGT 1 cut(s) 453
HpyCH4V TGCA 5 cut(s) 98, 105, 257, 492, 894
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 734
HpyF3I CTNAG 1 cut(s) 795
HpySE526I ACGT 1 cut(s) 453
Hsp92II CATG 7 cut(s) 114, 271, 347, 352, 463, 786, 907
HspAI GCGC 1 cut(s) 735
Kpn2I TCCGGA 1 cut(s) 875
Kzo9I GATC 2 cut(s) 34, 559
LmnI GCTCC 3 cut(s) 520, 670, 740
MaeI CTAG 5 cut(s) 153, 165, 177, 503, 699
MaeII ACGT 1 cut(s) 453
MaeIII GTNAC 3 cut(s) 295, 327, 862
MalI GATC 2 cut(s) 36, 561
MboI GATC 2 cut(s) 34, 559
MboII GAAGA 4 cut(s) 413, 817, 837, 915
MfeI CAATTG 1 cut(s) 889
MflI RGATCY 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 745
MluCI AATT 4 cut(s) 317, 511, 581, 889
MlyI GAGTC 3 cut(s) 439, 785, 841
MnlI CCTC 5 cut(s) 39, 70, 222, 696, 790
MroI TCCGGA 1 cut(s) 875
MroXI GAANNNNTTC 1 cut(s) 379
MseI TTAA 4 cut(s) 546, 711, 770, 955
MslI CAYNNNNRTG 1 cut(s) 941
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 2 cut(s) 227, 876
MspR9I CCNGG 1 cut(s) 498
MunI CAATTG 1 cut(s) 889
Mva1269I GAATGC 1 cut(s) 737
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 1 cut(s) 737
MwoI GCNNNNNNNGC 2 cut(s) 95, 734
NdeII GATC 2 cut(s) 34, 559
NlaIII CATG 7 cut(s) 114, 271, 347, 352, 463, 786, 907
NmuCI GTSAC 2 cut(s) 295, 862
NspI RCATGY 2 cut(s) 271, 347
PaeR7I CTCGAG 1 cut(s) 30
PagI TCATGA 1 cut(s) 903
PctI GAATGC 1 cut(s) 737
PdmI GAANNNNTTC 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 226
PkrI GCNGC 2 cut(s) 124, 263
PleI GAGTC 3 cut(s) 438, 785, 840
PpsI GAGTC 3 cut(s) 438, 785, 840
Ppu21I YACGTR 1 cut(s) 454
PshBI ATTAAT 1 cut(s) 546
Psp124BI GAGCTC 1 cut(s) 745
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PstI CTGCAG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 34
RsaI GTAC 5 cut(s) 163, 169, 175, 697, 722
RsaNI GTAC 5 cut(s) 162, 168, 174, 696, 721
RseI CAYNNNNRTG 1 cut(s) 941
SacI GAGCTC 1 cut(s) 745
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 4 cut(s) 546, 711, 770, 955
SatI GCNGC 2 cut(s) 123, 262
Sau3AI GATC 2 cut(s) 34, 559
ScaI AGTACT 2 cut(s) 163, 175
SchI GAGTC 3 cut(s) 439, 785, 841
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 1 cut(s) 745
SetI ASST 8 cut(s) 150, 279, 456, 508, 525, 660, 745, 801
SfcI CTRYAG 1 cut(s) 490
Sfr274I CTCGAG 1 cut(s) 30
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 1 cut(s) 941
SmlI CTYRAG 2 cut(s) 30, 829
SmoI CTYRAG 2 cut(s) 30, 829
SnaBI TACGTA 1 cut(s) 454
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 4 cut(s) 317, 511, 581, 889
SsiI CCGC 4 cut(s) 122, 261, 728, 737
SspI AATATT 1 cut(s) 544
SspMI CTAG 5 cut(s) 153, 165, 177, 503, 699
SstI GAGCTC 1 cut(s) 745
StyD4I CCNGG 1 cut(s) 496
TaaI ACNGT 4 cut(s) 301, 327, 599, 720
TaiI ACGT 1 cut(s) 456
TaqI TCGA 4 cut(s) 9, 31, 428, 643
TasI AATT 4 cut(s) 317, 511, 581, 889
TatI WGTACW 3 cut(s) 161, 173, 720
TauI GCSGC 2 cut(s) 125, 264
Tru1I TTAA 4 cut(s) 546, 711, 770, 955
Tru9I TTAA 4 cut(s) 546, 711, 770, 955
TscAI CASTG 5 cut(s) 187, 304, 562, 871, 933
TseFI GTSAC 2 cut(s) 295, 862
Tsp45I GTSAC 2 cut(s) 295, 862
TspDTI ATGAA 5 cut(s) 60, 365, 455, 892, 920
TspGWI ACGGA 2 cut(s) 35, 533
TspRI CASTG 5 cut(s) 187, 304, 562, 871, 933
VspI ATTAAT 1 cut(s) 546
XapI RAATTY 1 cut(s) 317
XceI RCATGY 2 cut(s) 271, 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 61
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 2 cut(s) 9, 17
XmnI GAANNNNTTC 1 cut(s) 379
XspI CTAG 5 cut(s) 153, 165, 177, 503, 699
ZrmI AGTACT 2 cut(s) 163, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.