Rw6G028120

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000399
Physical Location & Seq
Reverse (-)
52075147 .. 52076520
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G028120.1

Sequence Viewer

Length: 1287 bp
ATGTATAAAAGACCACGGGAGTCAAACTCTTCGGTCCTTCTAGTTTACCAGAGAAAAAGAAGAGTAATCTCGATGGCGCCCAACTGGTCGTACGATCTTCCCGAAGAGCTTATCATCTCTGTTGCCAAACGGCTGACAGGTTCCTTGCAGGATTTTATTTTTTTCGGTGCAGTTTGCAAATCATGGAGATCAGCCGCCACTGAGGTAAAGGAGAAAAACCCTAATAATATTGCATTGAGATTATTAACACATCAAGTTCCTCTCCTTTTGCTTCCACTTTCGTGTGGAATCTTGGTCAGCAACCCGGTTCCTAAAATTATCAGGCCGCCTCCCAAGGAAAAGAGTGAGGGTTTGAATGAGGCAGCTGAATTTTACAGTCTGAAGGGAAAAGGTAATAAGATCAGTTACAAACTTAACCTACCAAAAATATTACCAAAAGCAAGAATGAGGATATGCTTTTCATCTTTTGGATGGCTGGTGATTGTATCTAAAACTGAAGGGTGTGAATGGAGTCTGGTACATCCTTTAAATCATGCCAAAATTGAGCTCCCAGATGTACAGGGGTCTTTGCCAGCCAGCGTTGTTTCTGGGTTCCGGGTAGGGAAGTTTGCCTTATCATCGAGCCCTTCTTGGACATCGGATTACATAGTCATGTTTCAATCAGGTCAAAGATTTGGTTTTTGCAGGCCAGGGCTGGGTGAAAATTGGAAATACAAAGTATCAAGGGGAGATTTAGTAAGGGATCTGAGTTATTATAAAGGACAGTTCTATTTCGTGCACCTTTCCAGCCGCATTTCAGTTTTTGATATTGACAAAGAAGAAATAAGGCTTGTTGCTCCAGAATTTCCAAAGGAACAACTTTTAGGTCCTAATGTGCTTATTAAAGAGCACTATTTAGTGGAATCAGCGGGGGTTCTTTTGGTGGTTCTGTTCACTACCACTACCACCAAGTGTAAAGACCAAGGATTAACATTTGGCTGCAGGATTTTTGAGGTCCCATTTACAAACAGCAAATCGTGGTCGGACTCGGAGGTTAGGAGCTTGGGGAACAGAACCATTTTCCTAAGTAAATGTAGTTCTTCCTTCTCAATCGAGGCATCAGACTATTCTGGATTATGTAAGGCCAATTCCATCTACTTCATGAATAATAAGGTAGTCCTTCCGAGACAAAGCAGAGATATGGGTGTTTTTAATATGAAGGATGGAACAATCACCCGAGACTTTGAAAAATTCTTTGATTATGAAGAATTAAGAGGATTCCATTCATGGATTCAACCGAGTTTGTAA

Protein Analysis

428

Amino Acids

48.57

Weight (kDa)

9.41

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 124 - 398 2.5e-45 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 756
AccB1I GGYRCC 1 cut(s) 76
AciI CCGC 4 cut(s) 195, 326, 790, 908
AclWI GGATC 1 cut(s) 750
AcsI RAATTY 3 cut(s) 368, 842, 1229
AcuI CTGAAG 2 cut(s) 401, 516
AcyI GRCGYC 1 cut(s) 77
AdeI CACNNNGTG 1 cut(s) 951
AfaI GTAC 3 cut(s) 92, 519, 558
AfiI CCNNNNNNNGG 1 cut(s) 695
AgsI TTSAA 4 cut(s) 355, 659, 1226, 1274
AjnI CCWGG 1 cut(s) 688
AleI CACNNNNGTG 1 cut(s) 280
AluBI AGCT 4 cut(s) 109, 365, 547, 1041
AluI AGCT 4 cut(s) 109, 365, 547, 1041
Alw21I GWGCWC 3 cut(s) 549, 780, 891
Alw26I GTCTC 2 cut(s) 1159, 1212
Alw44I GTGCAC 1 cut(s) 776
AlwI GGATC 1 cut(s) 750
Ama87I CYCGRG 1 cut(s) 1215
AoxI GGCC 3 cut(s) 323, 686, 1122
ApaLI GTGCAC 1 cut(s) 776
ApeKI GCWGC 2 cut(s) 362, 978
ApoI RAATTY 3 cut(s) 368, 842, 1229
AspLEI GCGC 1 cut(s) 79
AspS9I GGNCC 3 cut(s) 34, 866, 994
AsuC2I CCSGG 2 cut(s) 305, 596
AsuHPI GGTGA 3 cut(s) 490, 710, 1204
AvaI CYCGRG 1 cut(s) 1215
AvaII GGWCC 3 cut(s) 34, 866, 994
BaeGI GKGCMC 1 cut(s) 780
BanI GGYRCC 1 cut(s) 76
BanII GRGCYC 2 cut(s) 549, 626
Bbv12I GWGCWC 3 cut(s) 549, 780, 891
BbvI GCAGC 2 cut(s) 374, 965
BccI CCATC 4 cut(s) 67, 465, 1139, 1196
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 690
BcnI CCSGG 2 cut(s) 305, 596
BcoDI GTCTC 2 cut(s) 1159, 1212
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 1 cut(s) 979
BfoI RGCGCY 1 cut(s) 80
BisI GCNGC 5 cut(s) 195, 326, 363, 790, 979
BlsI GCNGC 5 cut(s) 196, 327, 364, 791, 980
Bme1390I CCNGG 3 cut(s) 305, 596, 690
Bme18I GGWCC 3 cut(s) 34, 866, 994
BmeT110I CYCGRG 1 cut(s) 1215
BmgT120I GGNCC 3 cut(s) 34, 866, 994
BmiI GGNNCC 5 cut(s) 78, 142, 309, 593, 996
BmrFI CCNGG 3 cut(s) 305, 596, 690
BmsI GCATC 1 cut(s) 1106
BplI GAGNNNNNCTC 2 cut(s) 11, 43
BpmI CTGGAG 1 cut(s) 822
BpuMI CCSGG 2 cut(s) 305, 596
BsaHI GRCGYC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 14, 333, 689, 961
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse1I ACTGG 1 cut(s) 89
BseBI CCWGG 1 cut(s) 690
BseDI CCNNGG 4 cut(s) 14, 333, 689, 961
BseGI GGATG 3 cut(s) 476, 520, 1207
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMII CTCAG 2 cut(s) 192, 737
BseNI ACTGG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 780
BseXI GCAGC 2 cut(s) 374, 965
BseYI CCCAGC 1 cut(s) 694
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 3 cut(s) 325, 688, 1124
BshNI GGYRCC 1 cut(s) 76
BsiHKAI GWGCWC 3 cut(s) 549, 780, 891
BsiHKCI CYCGRG 1 cut(s) 1215
BsiSI CCGG 2 cut(s) 305, 595
BsiWI CGTACG 1 cut(s) 90
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 2 cut(s) 1159, 1212
BsmFI GGGAC 1 cut(s) 980
BsnI GGCC 3 cut(s) 325, 688, 1124
BsoBI CYCGRG 1 cut(s) 1215
Bsp1286I GDGCHC 4 cut(s) 549, 626, 780, 891
Bsp1407I TGTACA 1 cut(s) 556
Bsp143I GATC 4 cut(s) 94, 188, 399, 742
BspACI CCGC 4 cut(s) 195, 326, 790, 908
BspANI GGCC 3 cut(s) 325, 688, 1124
BspCNI CTCAG 2 cut(s) 193, 738
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 5 cut(s) 78, 142, 309, 593, 996
BspMAI CTGCAG 1 cut(s) 983
BspPI GGATC 1 cut(s) 750
BspQI GCTCTTC 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 556
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 4 cut(s) 14, 333, 689, 961
BssMI GATC 4 cut(s) 94, 188, 399, 742
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 333, 961
Bst2UI CCWGG 1 cut(s) 690
Bst4CI ACNGT 2 cut(s) 377, 765
Bst6I CTCTTC 3 cut(s) 34, 55, 99
BstACI GRCGYC 1 cut(s) 77
BstAUI TGTACA 1 cut(s) 556
BstC8I GCNNGC 3 cut(s) 573, 577, 686
BstDEI CTNAG 3 cut(s) 201, 746, 1064
BstDSI CCRYGG 1 cut(s) 14
BstF5I GGATG 3 cut(s) 476, 520, 1207
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 1 cut(s) 79
BstKTI GATC 4 cut(s) 97, 191, 402, 745
BstMAI GTCTC 2 cut(s) 1159, 1212
BstMBI GATC 4 cut(s) 94, 188, 399, 742
BstNI CCWGG 1 cut(s) 690
BstSCI CCNGG 3 cut(s) 303, 594, 688
BstSFI CTRYAG 1 cut(s) 979
BstSLI GKGCMC 1 cut(s) 780
BstV1I GCAGC 2 cut(s) 374, 965
BstX2I RGATCY 1 cut(s) 742
BstYI RGATCY 1 cut(s) 742
BsuRI GGCC 3 cut(s) 325, 688, 1124
BtgI CCRYGG 1 cut(s) 14
BtsCI GGATG 3 cut(s) 476, 520, 1207
BtsIMutI CAGTG 1 cut(s) 198
Cac8I GCNNGC 3 cut(s) 573, 577, 686
CciI TCATGA 1 cut(s) 1140
CfoI GCGC 1 cut(s) 79
Cfr13I GGNCC 3 cut(s) 34, 866, 994
Csp6I GTAC 3 cut(s) 91, 518, 557
CviAII CATG 5 cut(s) 183, 533, 652, 1141, 1266
CviQI GTAC 3 cut(s) 91, 518, 557
DdeI CTNAG 3 cut(s) 201, 746, 1064
DinI GGCGCC 1 cut(s) 78
DpnI GATC 4 cut(s) 96, 190, 401, 744
DpnII GATC 4 cut(s) 94, 188, 399, 742
DraI TTTAAA 1 cut(s) 528
DraIII CACNNNGTG 1 cut(s) 951
Eam1104I CTCTTC 3 cut(s) 34, 55, 99
EarI CTCTTC 3 cut(s) 34, 55, 99
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 2 cut(s) 333, 961
Eco24I GRGCYC 2 cut(s) 549, 626
Eco47I GGWCC 3 cut(s) 34, 866, 994
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 2 cut(s) 401, 516
Eco88I CYCGRG 1 cut(s) 1215
EcoICRI GAGCTC 1 cut(s) 547
EcoO109I RGGNCCY 2 cut(s) 866, 994
EcoRII CCWGG 1 cut(s) 688
EcoT14I CCWWGG 2 cut(s) 333, 961
EcoT38I GRGCYC 2 cut(s) 549, 626
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
ErhI CCWWGG 2 cut(s) 333, 961
FaeI CATG 5 cut(s) 186, 536, 655, 1144, 1269
FalI AAGNNNNNCTT 2 cut(s) 596, 628
FaqI GGGAC 1 cut(s) 980
FatI CATG 5 cut(s) 182, 532, 651, 1140, 1265
FauI CCCGC 1 cut(s) 901
Fnu4HI GCNGC 5 cut(s) 195, 326, 363, 790, 979
FokI GGATG 3 cut(s) 483, 507, 1214
FriOI GRGCYC 2 cut(s) 549, 626
Fsp4HI GCNGC 5 cut(s) 195, 326, 363, 790, 979
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 78
GluI GCNGC 5 cut(s) 195, 326, 363, 790, 979
GsaI CCCAGC 1 cut(s) 698
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 3 cut(s) 325, 688, 1124
HapII CCGG 2 cut(s) 305, 595
HhaI GCGC 1 cut(s) 79
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 5 cut(s) 186, 536, 655, 1144, 1269
Hin6I GCGC 1 cut(s) 77
HinP1I GCGC 1 cut(s) 77
HinfI GANTC 7 cut(s) 20, 288, 511, 902, 1025, 1257, 1270
HpaII CCGG 2 cut(s) 305, 595
HphI GGTGA 3 cut(s) 490, 710, 1204
Hpy166II GTNNAC 3 cut(s) 46, 778, 933
Hpy188I TCNGA 7 cut(s) 381, 640, 747, 1024, 1030, 1102, 1164
Hpy188III TCNNGA 5 cut(s) 70, 101, 839, 1110, 1141
Hpy8I GTNNAC 3 cut(s) 46, 778, 933
HpyAV CCTTC 7 cut(s) 47, 376, 491, 636, 1093, 1169, 1192
HpyCH4III ACNGT 2 cut(s) 377, 765
HpyCH4V TGCA 7 cut(s) 148, 170, 177, 233, 684, 778, 981
HpyF3I CTNAG 3 cut(s) 201, 746, 1064
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 5 cut(s) 186, 536, 655, 1144, 1269
HspAI GCGC 1 cut(s) 77
KasI GGCGCC 1 cut(s) 76
Kzo9I GATC 4 cut(s) 94, 188, 399, 742
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 3 cut(s) 552, 841, 1038
Lsp1109I GCAGC 2 cut(s) 374, 965
LweI GCATC 1 cut(s) 1106
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 404
MalI GATC 4 cut(s) 96, 190, 401, 744
MboI GATC 4 cut(s) 94, 188, 399, 742
MboII GAAGA 7 cut(s) 21, 72, 89, 116, 830, 1071, 1256
MflI RGATCY 1 cut(s) 742
MhlI GDGCHC 4 cut(s) 549, 626, 780, 891
MluCI AATT 8 cut(s) 315, 368, 540, 703, 842, 1126, 1229, 1247
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 3 cut(s) 29, 520, 1019
MmeI TCCRAC 1 cut(s) 1002
MseI TTAA 7 cut(s) 245, 414, 527, 882, 968, 1191, 1250
MslI CAYNNNNRTG 2 cut(s) 280, 650
MspA1I CMGCKG 2 cut(s) 365, 908
MspI CCGG 2 cut(s) 305, 595
MspR9I CCNGG 3 cut(s) 305, 596, 690
MvaI CCWGG 1 cut(s) 690
NarI GGCGCC 1 cut(s) 77
NciI CCSGG 2 cut(s) 305, 596
NdeII GATC 4 cut(s) 94, 188, 399, 742
NlaIII CATG 5 cut(s) 186, 536, 655, 1144, 1269
NlaIV GGNNCC 5 cut(s) 78, 142, 309, 593, 996
OliI CACNNNNGTG 1 cut(s) 280
PagI TCATGA 1 cut(s) 1140
PciSI GCTCTTC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PfeI GAWTC 4 cut(s) 288, 902, 1257, 1270
Pfl23II CGTACG 1 cut(s) 90
PkrI GCNGC 5 cut(s) 196, 327, 364, 791, 980
PleI GAGTC 3 cut(s) 28, 519, 1019
PluTI GGCGCC 1 cut(s) 80
PpsI GAGTC 3 cut(s) 28, 519, 1019
PpuMI RGGWCCY 2 cut(s) 866, 994
PsiI TTATAA 1 cut(s) 756
Psp124BI GAGCTC 1 cut(s) 549
Psp5II RGGWCCY 2 cut(s) 866, 994
Psp6I CCWGG 1 cut(s) 688
PspFI CCCAGC 1 cut(s) 694
PspGI CCWGG 1 cut(s) 688
PspLI CGTACG 1 cut(s) 90
PspN4I GGNNCC 5 cut(s) 78, 142, 309, 593, 996
PspPI GGNCC 3 cut(s) 34, 866, 994
PspPPI RGGWCCY 2 cut(s) 866, 994
PsrI GAACNNNNNNTAC 2 cut(s) 1060, 1092
PstI CTGCAG 1 cut(s) 983
PsuI RGATCY 1 cut(s) 742
PvuII CAGCTG 1 cut(s) 365
RsaI GTAC 3 cut(s) 92, 519, 558
RsaNI GTAC 3 cut(s) 91, 518, 557
RseI CAYNNNNRTG 2 cut(s) 280, 650
SacI GAGCTC 1 cut(s) 549
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 7 cut(s) 245, 414, 527, 882, 968, 1191, 1250
SatI GCNGC 5 cut(s) 195, 326, 363, 790, 979
Sau3AI GATC 4 cut(s) 94, 188, 399, 742
Sau96I GGNCC 3 cut(s) 34, 866, 994
SchI GAGTC 3 cut(s) 29, 520, 1019
ScrFI CCNGG 3 cut(s) 305, 596, 690
SduI GDGCHC 4 cut(s) 549, 626, 780, 891
SfaNI GCATC 1 cut(s) 1106
SfcI CTRYAG 1 cut(s) 979
SfoI GGCGCC 1 cut(s) 78
SinI GGWCC 3 cut(s) 34, 866, 994
SmiMI CAYNNNNRTG 2 cut(s) 280, 650
Sse9I AATT 8 cut(s) 315, 368, 540, 703, 842, 1126, 1229, 1247
SsiI CCGC 4 cut(s) 195, 326, 790, 908
SspDI GGCGCC 1 cut(s) 76
SspI AATATT 2 cut(s) 229, 429
SspMI CTAG 1 cut(s) 41
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 3 cut(s) 303, 594, 688
StyI CCWWGG 2 cut(s) 333, 961
TaaI ACNGT 2 cut(s) 377, 765
TaqI TCGA 3 cut(s) 71, 620, 1092
TaqII GACCGA 1 cut(s) 22
TasI AATT 8 cut(s) 315, 368, 540, 703, 842, 1126, 1229, 1247
TatI WGTACW 1 cut(s) 556
TauI GCSGC 3 cut(s) 197, 328, 792
TfiI GAWTC 4 cut(s) 288, 902, 1257, 1270
Tru1I TTAA 7 cut(s) 245, 414, 527, 882, 968, 1191, 1250
Tru9I TTAA 7 cut(s) 245, 414, 527, 882, 968, 1191, 1250
TscAI CASTG 1 cut(s) 205
TseI GCWGC 2 cut(s) 362, 978
TspDTI ATGAA 6 cut(s) 450, 1129, 1157, 1211, 1254, 1257
TspRI CASTG 1 cut(s) 205
VneI GTGCAC 1 cut(s) 776
VpaK11BI GGWCC 3 cut(s) 34, 866, 994
XapI RAATTY 3 cut(s) 368, 842, 1229
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.