MD05G1357500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000399
Physical Location & Seq
Forward (+)
47326276 .. 47327439
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1357500.v1.1.491

Sequence Viewer

Length: 1164 bp
ATGGCTCTCGTTTGTCCAATAAAACGTATGCCTCCCTGGTCGGTGCTTCCTCACGATCTTATAGCTTCTATCGCCAAGCGCTTGTTTGCCTTCGAAGATTTTATTGCTTTTGGTGCCGTTTGTACATCATGGAAATCAGCTGCGACCAAAGAAAACTTTACTACAAGACGTCAAATTCCATTACTCATGCTTCCGGAGAAGGAGAAGGAGAAGGAGAGCACCACCACCACCACCACCGCTACCTCTACAGTGCGTGAGTTCTACAACTTGAAAAACGGTAAGGTATTCCAACTTAATATGCCAGACTTAGTTAGGGGAAAGTTGTGTTATTCTTCTCTAGGATGGCTAATGACTCAATCTGATAAAGACTCGAAACTCGATCTCTTGCATCCTTTAAACCATTCTCGTATCGAGCTTCCAGACGCTGAAACATTTAAGAAACATCACTCGGACTTGGACCTGTCTAATTTGAAAACGCTGTTTGGTGATTTCCTCGTAAGGAAATTTGTCTTGTCGTCGAGCCCTTCTTGTTCGTCCGACTACACAGTCATGATACATAGTTGGGAAAGACTGGCATTTTGTAGACCGGGAATTGGCGACCAACAATGGAACGTCCTTATGAAGTCAGAAAAAGAAGATCACTTGTATTTTGATATAACGTACTATAAGGGAAAGTTTTATGCCGTGGACGCTTATGGTACAATCTTGGTATGTGAAATTGAAGATCCCATGCAAGCAAGGACGAGGATTATTGTTCCAAAGTTGCCGGAAGAACTTGGATTCCCTTTCCTCTGCGAGAAAGAGTACCTTGTGGAATCAGCAGGGGCCTTATTGCTCGTCACGCGTTCTTTTATACCTACCTTTGAGTTTAGGGTTTTCGAGGTGCCATTGAGCAACGGAGATTGGTCCGACGACGCTAGGGTTAAAAGCCTGGGAAATAGAACTCTATTCCTAGGTTATAATTCTTCATTTTCTGTCGAGTCCTCAAACTACGGATGTAAAGCTAATTGCATATATTACACGAATCATCCGCCAGCACTTGTTCGTCAAGAGGAAGAGATAGACATGGGTATTTTTTATATGGAGGACGGAAAGGCCGAGGCTGACCTAAGAGAACCCATCAATTCCCTAACTCCACATTTGTGGGTTGAGCCGAGCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box

Protein Analysis

388

Amino Acids

44.28

Weight (kDa)

5.69

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 87 - 359 8.1e-57 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 960
AasI GACNNNNNNGTC 1 cut(s) 545
AatII GACGTC 1 cut(s) 172
AccB1I GGYRCC 2 cut(s) 113, 883
AccI GTMKAC 1 cut(s) 583
AccII CGCG 1 cut(s) 844
AccIII TCCGGA 1 cut(s) 193
AciI CCGC 2 cut(s) 237, 1031
AclWI GGATC 1 cut(s) 719
AcsI RAATTY 2 cut(s) 174, 503
AcyI GRCGYC 1 cut(s) 169
AfaI GTAC 4 cut(s) 124, 662, 700, 806
AfeI AGCGCT 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 593
AflIII ACRYGT 1 cut(s) 842
AgsI TTSAA 3 cut(s) 271, 472, 722
AjnI CCWGG 2 cut(s) 35, 930
AleI CACNNNNGTG 1 cut(s) 1141
AloI GAACNNNNNNTCC 2 cut(s) 765, 797
AluBI AGCT 5 cut(s) 65, 140, 415, 1004, 1158
AluI AGCT 5 cut(s) 65, 140, 415, 1004, 1158
Alw21I GWGCWC 1 cut(s) 221
AlwI GGATC 1 cut(s) 719
AlwNI CAGNNNCTG 1 cut(s) 425
Aor13HI TCCGGA 1 cut(s) 193
Aor51HI AGCGCT 1 cut(s) 80
AoxI GGCC 2 cut(s) 825, 1095
ApeKI GCWGC 1 cut(s) 140
ApoI RAATTY 2 cut(s) 174, 503
ArsI GACNNNNNNTTYG 2 cut(s) 561, 593
AspA2I CCTAGG 1 cut(s) 952
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 3 cut(s) 457, 825, 906
AsuC2I CCSGG 1 cut(s) 588
AsuHPI GGTGA 1 cut(s) 497
AsuII TTCGAA 1 cut(s) 93
AvaII GGWCC 2 cut(s) 457, 906
AvrII CCTAGG 1 cut(s) 952
BanI GGYRCC 2 cut(s) 113, 883
BanII GRGCYC 1 cut(s) 524
Bbv12I GWGCWC 1 cut(s) 221
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 336, 1127
BceAI ACGGC 2 cut(s) 101, 668
BciT130I CCWGG 2 cut(s) 37, 932
BcnI CCSGG 1 cut(s) 588
BfaI CTAG 3 cut(s) 338, 918, 953
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 82
BisI GCNGC 1 cut(s) 141
BlnI CCTAGG 1 cut(s) 952
BlsI GCNGC 1 cut(s) 142
Bme1390I CCNGG 3 cut(s) 37, 588, 932
Bme18I GGWCC 2 cut(s) 457, 906
BmgT120I GGNCC 3 cut(s) 457, 825, 906
BmiI GGNNCC 3 cut(s) 115, 826, 885
BmrFI CCNGG 3 cut(s) 37, 588, 932
BmsI GCATC 1 cut(s) 397
Bpu14I TTCGAA 1 cut(s) 93
BpuMI CCSGG 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 169
BsaJI CCNNGG 5 cut(s) 35, 684, 931, 952, 1098
BsaWI WCCGGW 1 cut(s) 193
Bsc4I CCNNNNNNNGG 1 cut(s) 593
Bse1I ACTGG 1 cut(s) 576
BseAI TCCGGA 1 cut(s) 193
BseBI CCWGG 2 cut(s) 37, 932
BseDI CCNNGG 5 cut(s) 35, 684, 931, 952, 1098
BseGI GGATG 4 cut(s) 347, 388, 1001, 1027
BseLI CCNNNNNNNGG 1 cut(s) 593
BseNI ACTGG 1 cut(s) 576
BseXI GCAGC 1 cut(s) 127
Bsh1236I CGCG 1 cut(s) 844
BshFI GGCC 2 cut(s) 827, 1097
BshNI GGYRCC 2 cut(s) 113, 883
BsiHKAI GWGCWC 1 cut(s) 221
BsiSI CCGG 3 cut(s) 194, 587, 767
BslI CCNNNNNNNGG 1 cut(s) 593
BsnI GGCC 2 cut(s) 827, 1097
Bsp119I TTCGAA 1 cut(s) 93
Bsp1286I GDGCHC 2 cut(s) 221, 524
Bsp13I TCCGGA 1 cut(s) 193
Bsp1407I TGTACA 1 cut(s) 122
Bsp143I GATC 4 cut(s) 55, 379, 637, 724
BspACI CCGC 2 cut(s) 237, 1031
BspANI GGCC 2 cut(s) 827, 1097
BspEI TCCGGA 1 cut(s) 193
BspFNI CGCG 1 cut(s) 844
BspHI TCATGA 1 cut(s) 549
BspLI GGNNCC 3 cut(s) 115, 826, 885
BspPI GGATC 1 cut(s) 719
BspT104I TTCGAA 1 cut(s) 93
BspT107I GGYRCC 2 cut(s) 113, 883
BsrGI TGTACA 1 cut(s) 122
BsrI ACTGG 1 cut(s) 576
BssECI CCNNGG 5 cut(s) 35, 684, 931, 952, 1098
BssMI GATC 4 cut(s) 55, 379, 637, 724
BssNI GRCGYC 1 cut(s) 169
BssT1I CCWWGG 1 cut(s) 952
Bst2UI CCWGG 2 cut(s) 37, 932
Bst4CI ACNGT 3 cut(s) 250, 278, 547
Bst6I CTCTTC 1 cut(s) 1050
BstACI GRCGYC 1 cut(s) 169
BstAUI TGTACA 1 cut(s) 122
BstBI TTCGAA 1 cut(s) 93
BstC8I GCNNGC 2 cut(s) 735, 1035
BstDEI CTNAG 2 cut(s) 307, 1109
BstDSI CCRYGG 1 cut(s) 684
BstF5I GGATG 4 cut(s) 347, 388, 1001, 1027
BstFNI CGCG 1 cut(s) 844
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 4 cut(s) 58, 382, 640, 727
BstMBI GATC 4 cut(s) 55, 379, 637, 724
BstMWI GCNNNNNNNGC 4 cut(s) 71, 113, 689, 841
BstNI CCWGG 2 cut(s) 37, 932
BstSCI CCNGG 3 cut(s) 35, 586, 930
BstSFI CTRYAG 1 cut(s) 246
BstUI CGCG 1 cut(s) 844
BstV1I GCAGC 1 cut(s) 127
BstX2I RGATCY 1 cut(s) 724
BstXI CCANNNNNNTGG 1 cut(s) 1143
BstYI RGATCY 1 cut(s) 724
BsuRI GGCC 2 cut(s) 827, 1097
BtgI CCRYGG 1 cut(s) 684
BtsCI GGATG 4 cut(s) 347, 388, 1001, 1027
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 735, 1035
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 549
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 3 cut(s) 457, 825, 906
CseI GACGC 3 cut(s) 431, 698, 923
Csp6I GTAC 4 cut(s) 123, 661, 699, 805
CviAII CATG 5 cut(s) 129, 187, 550, 730, 1066
CviQI GTAC 4 cut(s) 123, 661, 699, 805
DdeI CTNAG 2 cut(s) 307, 1109
DpnI GATC 4 cut(s) 57, 381, 639, 726
DpnII GATC 4 cut(s) 55, 379, 637, 724
DraI TTTAAA 1 cut(s) 396
DrdI GACNNNNNNGTC 1 cut(s) 545
DseDI GACNNNNNNGTC 1 cut(s) 545
Eam1104I CTCTTC 1 cut(s) 1050
EarI CTCTTC 1 cut(s) 1050
EciI GGCGGA 1 cut(s) 1020
Eco130I CCWWGG 1 cut(s) 952
Eco24I GRGCYC 1 cut(s) 524
Eco47I GGWCC 2 cut(s) 457, 906
Eco47III AGCGCT 1 cut(s) 80
EcoO109I RGGNCCY 1 cut(s) 825
EcoRII CCWGG 2 cut(s) 35, 930
EcoT14I CCWWGG 1 cut(s) 952
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 952
FaeI CATG 5 cut(s) 132, 190, 553, 733, 1069
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FatI CATG 5 cut(s) 128, 186, 549, 729, 1065
FblI GTMKAC 1 cut(s) 583
Fnu4HI GCNGC 1 cut(s) 141
FokI GGATG 4 cut(s) 354, 375, 1008, 1014
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 1 cut(s) 141
FspBI CTAG 3 cut(s) 338, 918, 953
GlaI GCGC 1 cut(s) 80
GluI GCNGC 1 cut(s) 141
HaeII RGCGCY 1 cut(s) 82
HaeIII GGCC 2 cut(s) 827, 1097
HapII CCGG 3 cut(s) 194, 587, 767
HgaI GACGC 3 cut(s) 431, 698, 923
HhaI GCGC 1 cut(s) 81
Hin1I GRCGYC 1 cut(s) 169
Hin1II CATG 5 cut(s) 132, 190, 553, 733, 1069
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HinfI GANTC 6 cut(s) 352, 368, 780, 815, 980, 1024
HpaII CCGG 3 cut(s) 194, 587, 767
HphI GGTGA 1 cut(s) 497
Hpy166II GTNNAC 2 cut(s) 584, 688
Hpy188I TCNGA 5 cut(s) 361, 451, 538, 628, 910
Hpy188III TCNNGA 5 cut(s) 53, 194, 419, 550, 1049
Hpy8I GTNNAC 2 cut(s) 584, 688
Hpy99I CGWCG 3 cut(s) 520, 914, 917
HpyAV CCTTC 5 cut(s) 100, 193, 199, 205, 534
HpyCH4III ACNGT 3 cut(s) 250, 278, 547
HpyCH4IV ACGT 4 cut(s) 25, 169, 612, 659
HpyCH4V TGCA 3 cut(s) 388, 733, 1011
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 113, 689, 841
HpyF3I CTNAG 2 cut(s) 307, 1109
HpySE526I ACGT 4 cut(s) 25, 169, 612, 659
Hsp92I GRCGYC 1 cut(s) 169
Hsp92II CATG 5 cut(s) 132, 190, 553, 733, 1069
HspAI GCGC 1 cut(s) 79
Kpn2I TCCGGA 1 cut(s) 193
Kzo9I GATC 4 cut(s) 55, 379, 637, 724
Lsp1109I GCAGC 1 cut(s) 127
LweI GCATC 1 cut(s) 397
MaeI CTAG 3 cut(s) 338, 918, 953
MaeII ACGT 4 cut(s) 25, 169, 612, 659
MaeIII GTNAC 1 cut(s) 838
MalI GATC 4 cut(s) 57, 381, 639, 726
MboI GATC 4 cut(s) 55, 379, 637, 724
MboII GAAGA 7 cut(s) 107, 324, 647, 734, 782, 957, 1067
MflI RGATCY 1 cut(s) 724
MhlI GDGCHC 2 cut(s) 221, 524
MluCI AATT 8 cut(s) 174, 466, 503, 591, 717, 961, 1006, 1123
MluI ACGCGT 1 cut(s) 842
MlyI GAGTC 3 cut(s) 346, 362, 989
MmeI TCCRAC 3 cut(s) 313, 561, 933
MroI TCCGGA 1 cut(s) 193
MseI TTAA 5 cut(s) 294, 395, 435, 924, 1162
MslI CAYNNNNRTG 2 cut(s) 548, 1141
MspA1I CMGCKG 1 cut(s) 140
MspI CCGG 3 cut(s) 194, 587, 767
MspR9I CCNGG 3 cut(s) 37, 588, 932
MvaI CCWGG 2 cut(s) 37, 932
MvnI CGCG 1 cut(s) 844
MwoI GCNNNNNNNGC 4 cut(s) 71, 113, 689, 841
NciI CCSGG 1 cut(s) 588
NdeII GATC 4 cut(s) 55, 379, 637, 724
NlaIII CATG 5 cut(s) 132, 190, 553, 733, 1069
NlaIV GGNNCC 3 cut(s) 115, 826, 885
NmeAIII GCCGAG 1 cut(s) 1123
NmuCI GTSAC 1 cut(s) 838
NspV TTCGAA 1 cut(s) 93
OliI CACNNNNGTG 1 cut(s) 1141
PagI TCATGA 1 cut(s) 549
PcsI WCGNNNNNNNCGW 1 cut(s) 1095
PfeI GAWTC 3 cut(s) 780, 815, 1024
PkrI GCNGC 1 cut(s) 142
PleI GAGTC 3 cut(s) 346, 362, 988
PpsI GAGTC 3 cut(s) 346, 362, 988
PsiI TTATAA 1 cut(s) 960
Psp6I CCWGG 2 cut(s) 35, 930
PspGI CCWGG 2 cut(s) 35, 930
PspN4I GGNNCC 3 cut(s) 115, 826, 885
PspPI GGNCC 3 cut(s) 457, 825, 906
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 724
PvuII CAGCTG 1 cut(s) 140
RsaI GTAC 4 cut(s) 124, 662, 700, 806
RsaNI GTAC 4 cut(s) 123, 661, 699, 805
RseI CAYNNNNRTG 2 cut(s) 548, 1141
SaqAI TTAA 5 cut(s) 294, 395, 435, 924, 1162
SatI GCNGC 1 cut(s) 141
Sau3AI GATC 4 cut(s) 55, 379, 637, 724
Sau96I GGNCC 3 cut(s) 457, 825, 906
SchI GAGTC 3 cut(s) 346, 362, 989
ScrFI CCNGG 3 cut(s) 37, 588, 932
SduI GDGCHC 2 cut(s) 221, 524
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 93
SinI GGWCC 2 cut(s) 457, 906
SmiMI CAYNNNNRTG 2 cut(s) 548, 1141
Sse9I AATT 8 cut(s) 174, 466, 503, 591, 717, 961, 1006, 1123
SsiI CCGC 2 cut(s) 237, 1031
SspMI CTAG 3 cut(s) 338, 918, 953
StyD4I CCNGG 3 cut(s) 35, 586, 930
StyI CCWWGG 1 cut(s) 952
TaaI ACNGT 3 cut(s) 250, 278, 547
TaiI ACGT 4 cut(s) 28, 172, 615, 662
TaqI TCGA 7 cut(s) 93, 371, 378, 411, 518, 879, 978
TasI AATT 8 cut(s) 174, 466, 503, 591, 717, 961, 1006, 1123
TatI WGTACW 1 cut(s) 122
TfiI GAWTC 3 cut(s) 780, 815, 1024
Tru1I TTAA 5 cut(s) 294, 395, 435, 924, 1162
Tru9I TTAA 5 cut(s) 294, 395, 435, 924, 1162
TscAI CASTG 1 cut(s) 255
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 1 cut(s) 140
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 2 cut(s) 635, 957
TspGWI ACGGA 3 cut(s) 912, 1008, 1104
TspRI CASTG 1 cut(s) 255
VpaK11BI GGWCC 2 cut(s) 457, 906
XapI RAATTY 2 cut(s) 174, 503
XmaJI CCTAGG 1 cut(s) 952
XmiI GTMKAC 1 cut(s) 583
XspI CTAG 3 cut(s) 338, 918, 953
ZraI GACGTC 1 cut(s) 170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.