Rmu_sc0003335.1_g000007

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003335.1
OG0000399
Physical Location & Seq
Reverse (-)
29902 .. 30966
1065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003335.1_g000007.1.cds

Sequence Viewer

Length: 1065 bp
atggctaccgggtggtgcgattctcttccgaattaccttgtctctctcggcgaaaggctggcagttttctggaacaagtttatcactgcagtgcggaaatcattcggcgctgatcaagttcctcttcttatgcttccaaagaggattgatgacgccatggttcagttttacaccctcgcaaaaggtgagatctacaacgccaatctcccagaaatcagtggtaaggcctgctttgcttccctgggatggctgcttactgtaactagtgatcaaggggaattgaaatttaatatgctccacccatcaaatcatcatgcccaaattcaacttcctggtttgctgcgctgtccgctcgagaatatttggaagtttgctttgtcgtccaatcctcgttccacattggattatatagtcatgtttcagacagctactactttgggattttgcagaccaggactgggcggccaaggttggaaacatctcagatttgagggctacaatttcattagagatctcacacactatcaaggaagtttctacgttgtggacgagactggccgtgtttctgttgtttgtgaaattgaagaccctaagcgagcacatttgagtgttgttgctccagagattccgagtgaggagcaacttctaggtcctaagaagggcattaaacagctatatctggtggaatctgcagggtccctattggtggttttgtgtacttccttccataatcattatgaatcaactattggctgcagggttttcaaggtcccattcagtaatggcaattcatggtccgactcagaggtaaagaatttgggtaatagagccctcttcttgagtaacagcagttcttcattctctattgagggttcaggcaattccggatgcaagcccaattgcatatatttcctgaacagcaaaatggtctctccattcgttgacttagatgttggtatttttaatatggaggatgggacaattgatcgagacttcagcaattccttcaataccgggtttgaagaaatcagagaatcccatctgtggattcaaccgagtttctga

Protein Analysis

354

Amino Acids

39.57

Weight (kDa)

6.0

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000399)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g21230 FvH4_2g22763 FvH4_2g23250 FvH4_3g19214 FvH4_5g01761 FvH4_5g02180 FvH4_5g23981 FvH4_7g21350 FvH4_7g21590
malus_domestica MD00G1040400.v1.1 MD00G1057500.v1.1 MD05G1079000.v1.1 MD05G1357500.v1.1 MD05G1357800.v1.1
prunus_persica Prupe.4G213300_v2.0.a1 Prupe.8G190800_v2.0.a1 Prupe.8G190900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0372911 RchiOBHm_Chr5g0006331 RchiOBHm_Chr5g0032291 RchiOBHm_Chr6g0287741 RchiOBHm_Chr6g0287791 RchiOBHm_Chr6g0289281 RchiOBHm_Chr6g0290581 RchiOBHm_Chr6g0290601 RchiOBHm_Chr7g0210061
rosa_laevigata RLG00000003898 RLG00000004101 RLG00000012190 RLG00000012194 RLG00000012196 RLG00000012198 RLG00000012199 RLG00000012200 RLG00000012278 RLG00000012316 RLG00000012346 RLG00000012468 RLG00000027389 RLG00000033378
rosa_multiflora Rmu_sc0000366.1_g000019 Rmu_sc0001082.1_g000011 Rmu_sc0001105.1_g000009 Rmu_sc0002290.1_g000001 Rmu_sc0003335.1_g000005 Rmu_sc0003335.1_g000007 Rmu_sc0003877.1_g000009 Rmu_sc0003877.1_g000011 Rmu_sc0004311.1_g000002 Rmu_sc0006266.1_g000019 Rmu_sc0006802.1_g000001 Rmu_sc0008789.1_g000004 Rmu_sc0010418.1_g000008 Rmu_sc0016104.1_g000004 Rmu_sc0022792.1_g000002 Rmu_sc0030012.1_g000001
rosa_roxburghii Rroxscaffold_3G00248640 Rroxscaffold_3G00258060 Rroxscaffold_4G00284630 Rroxscaffold_4G00291560 Rroxscaffold_7G00172250 Rroxscaffold_7G00176870 Rroxscaffold_7G00176900 Rroxscaffold_7G00176910 Rroxscaffold_7G00179370 Rroxscaffold_7G00181120 Rroxscaffold_7G00181140 Rroxscaffold_7G00181220
rosa_rugosa Rorug01G0324600 Rorug01G0324900 Rorug05G0130400 Rorug06G0191400 Rorug06G0191700 Rorug06G0192000 Rorug06G0192100 Rorug06G0199500 Rorug06G0199600 Rorug06G0210400 Rorug06G0213400 Rorug07G0044900
rosa_samantha Rh1BG289300 Rh5CG250500 Rh6DG111000 Rh6DG299100 Rh6DG299400 Rh6DG299700 Rh6DG300000 Rh6DG324600 Rh7BG171300
rosa_wichuraiana Rw0G015350 Rw1G029080 Rw1G029600 Rw5G020460 Rw6G026040 Rw6G028110 Rw6G028120 Rw6G028150 Rw6G031990 Rw7G014690 Rw7G014700 Rw7G015310

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 352
AccIII TCCGGA 1 cut(s) 884
AciI CCGC 3 cut(s) 94, 350, 462
AcoI YGGCCR 2 cut(s) 463, 556
AcsI RAATTY 3 cut(s) 284, 321, 814
AcuI CTGAAG 1 cut(s) 979
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 1 cut(s) 718
AfiI CCNNNNNNNGG 5 cut(s) 246, 458, 659, 706, 1044
AgsI TTSAA 7 cut(s) 283, 326, 584, 766, 1009, 1022, 1052
AhlI ACTAGT 1 cut(s) 263
AjnI CCWGG 3 cut(s) 240, 331, 451
AjuI GAANNNNNNNTTGG 4 cut(s) 312, 344, 732, 764
AleI CACNNNNGTG 1 cut(s) 89
AluBI AGCT 2 cut(s) 428, 673
AluI AGCT 2 cut(s) 428, 673
Alw21I GWGCWC 1 cut(s) 601
Alw26I GTCTC 4 cut(s) 46, 545, 934, 984
Ama87I CYCGRG 1 cut(s) 353
Aor13HI TCCGGA 1 cut(s) 884
AoxI GGCC 3 cut(s) 225, 463, 556
ApeKI GCWGC 3 cut(s) 250, 340, 753
ApoI RAATTY 3 cut(s) 284, 321, 814
Asp700I GAANNNNTTC 1 cut(s) 101
AspLEI GCGC 2 cut(s) 110, 345
AspS9I GGNCC 4 cut(s) 650, 696, 769, 795
AsuC2I CCSGG 2 cut(s) 10, 1015
AsuHPI GGTGA 1 cut(s) 197
AvaI CYCGRG 1 cut(s) 353
AvaII GGWCC 4 cut(s) 650, 696, 769, 795
BanII GRGCYC 1 cut(s) 832
BbsI GAAGAC 1 cut(s) 591
Bbv12I GWGCWC 1 cut(s) 601
BbvI GCAGC 3 cut(s) 237, 327, 740
BccI CCATC 4 cut(s) 240, 310, 968, 1047
BceAI ACGGC 1 cut(s) 543
BciT130I CCWGG 3 cut(s) 242, 333, 453
BclI TGATCA 2 cut(s) 112, 268
BcnI CCSGG 2 cut(s) 10, 1015
BcoDI GTCTC 4 cut(s) 46, 545, 934, 984
BcuI ACTAGT 1 cut(s) 263
BfaI CTAG 2 cut(s) 264, 647
BfmI CTRYAG 3 cut(s) 87, 690, 754
BfoI RGCGCY 1 cut(s) 111
BglII AGATCT 2 cut(s) 189, 511
BisI GCNGC 4 cut(s) 251, 341, 463, 754
BlsI GCNGC 4 cut(s) 252, 342, 464, 755
Bme1390I CCNGG 5 cut(s) 10, 242, 333, 453, 1015
Bme18I GGWCC 4 cut(s) 650, 696, 769, 795
BmeT110I CYCGRG 1 cut(s) 353
BmgT120I GGNCC 4 cut(s) 650, 696, 769, 795
BmiI GGNNCC 3 cut(s) 697, 698, 771
BmrFI CCNGG 5 cut(s) 10, 242, 333, 453, 1015
BmrI ACTGGG 1 cut(s) 467
BmsI GCATC 1 cut(s) 878
BmuI ACTGGG 1 cut(s) 467
BpiI GAAGAC 1 cut(s) 591
BpmI CTGGAG 1 cut(s) 603
Bpu10I CCTNAGC 1 cut(s) 591
BpuEI CTTGAG 1 cut(s) 859
BpuMI CCSGG 2 cut(s) 10, 1015
BsaHI GRCGYC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 934
BsaJI CCNNGG 4 cut(s) 156, 240, 241, 466
BsaWI WCCGGW 1 cut(s) 884
Bsc4I CCNNNNNNNGG 5 cut(s) 246, 458, 659, 706, 1044
Bse1I ACTGG 2 cut(s) 462, 559
BseAI TCCGGA 1 cut(s) 884
BseBI CCWGG 3 cut(s) 242, 333, 453
BseDI CCNNGG 4 cut(s) 156, 240, 241, 466
BseGI GGATG 3 cut(s) 251, 893, 979
BseLI CCNNNNNNNGG 5 cut(s) 246, 458, 659, 706, 1044
BseMII CTCAG 2 cut(s) 496, 816
BseNI ACTGG 2 cut(s) 462, 559
BseRI GAGGAG 1 cut(s) 650
BseXI GCAGC 3 cut(s) 237, 327, 740
BshFI GGCC 3 cut(s) 227, 465, 558
BsiHKAI GWGCWC 1 cut(s) 601
BsiHKCI CYCGRG 1 cut(s) 353
BsiSI CCGG 3 cut(s) 9, 885, 1014
BslFI GGGAC 3 cut(s) 682, 755, 991
BslI CCNNNNNNNGG 5 cut(s) 246, 458, 659, 706, 1044
BsmAI GTCTC 4 cut(s) 46, 545, 934, 984
BsmFI GGGAC 3 cut(s) 682, 755, 991
BsnI GGCC 3 cut(s) 227, 465, 558
Bso31I GGTCTC 1 cut(s) 934
BsoBI CYCGRG 1 cut(s) 353
Bsp1286I GDGCHC 2 cut(s) 601, 832
Bsp13I TCCGGA 1 cut(s) 884
Bsp143I GATC 5 cut(s) 112, 189, 268, 511, 985
Bsp19I CCATGG 1 cut(s) 156
BspACI CCGC 3 cut(s) 94, 350, 462
BspANI GGCC 3 cut(s) 227, 465, 558
BspCNI CTCAG 2 cut(s) 495, 815
BspEI TCCGGA 1 cut(s) 884
BspLI GGNNCC 3 cut(s) 697, 698, 771
BspMAI CTGCAG 3 cut(s) 91, 694, 758
BspTNI GGTCTC 1 cut(s) 934
BsrBI CCGCTC 1 cut(s) 352
BsrI ACTGG 2 cut(s) 462, 559
BssECI CCNNGG 4 cut(s) 156, 240, 241, 466
BssMI GATC 5 cut(s) 112, 189, 268, 511, 985
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 2 cut(s) 156, 466
Bst2UI CCWGG 3 cut(s) 242, 333, 453
Bst4CI ACNGT 1 cut(s) 259
Bst6I CTCTTC 3 cut(s) 30, 129, 839
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 4 cut(s) 60, 229, 597, 893
BstDEI CTNAG 5 cut(s) 482, 591, 654, 802, 946
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 3 cut(s) 251, 893, 979
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 2 cut(s) 110, 345
BstKTI GATC 5 cut(s) 115, 192, 271, 514, 988
BstMAI GTCTC 4 cut(s) 46, 545, 934, 984
BstMBI GATC 5 cut(s) 112, 189, 268, 511, 985
BstMWI GCNNNNNNNGC 2 cut(s) 233, 349
BstNI CCWGG 3 cut(s) 242, 333, 453
BstSCI CCNGG 5 cut(s) 8, 240, 331, 451, 1013
BstSFI CTRYAG 3 cut(s) 87, 690, 754
BstV1I GCAGC 3 cut(s) 237, 327, 740
BstV2I GAAGAC 1 cut(s) 591
BstX2I RGATCY 2 cut(s) 189, 511
BstYI RGATCY 2 cut(s) 189, 511
BsuRI GGCC 3 cut(s) 227, 465, 558
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 3 cut(s) 251, 893, 979
BtsI GCAGTG 2 cut(s) 84, 96
BtsIMutI CAGTG 3 cut(s) 84, 96, 223
Cac8I GCNNGC 4 cut(s) 60, 229, 597, 893
CfoI GCGC 2 cut(s) 110, 345
Cfr13I GGNCC 4 cut(s) 650, 696, 769, 795
CseI GACGC 1 cut(s) 161
Csp6I GTAC 1 cut(s) 717
CviAII CATG 4 cut(s) 157, 314, 415, 792
CviQI GTAC 1 cut(s) 717
DdeI CTNAG 5 cut(s) 482, 591, 654, 802, 946
DpnI GATC 5 cut(s) 114, 191, 270, 513, 987
DpnII GATC 5 cut(s) 112, 189, 268, 511, 985
EaeI YGGCCR 2 cut(s) 463, 556
Eam1104I CTCTTC 3 cut(s) 30, 129, 839
EarI CTCTTC 3 cut(s) 30, 129, 839
Eco130I CCWWGG 2 cut(s) 156, 466
Eco147I AGGCCT 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 832
Eco31I GGTCTC 1 cut(s) 934
Eco47I GGWCC 4 cut(s) 650, 696, 769, 795
Eco57I CTGAAG 1 cut(s) 979
Eco88I CYCGRG 1 cut(s) 353
EcoO109I RGGNCCY 3 cut(s) 650, 696, 769
EcoRII CCWGG 3 cut(s) 240, 331, 451
EcoT14I CCWWGG 2 cut(s) 156, 466
EcoT38I GRGCYC 1 cut(s) 832
ErhI CCWWGG 2 cut(s) 156, 466
FaeI CATG 4 cut(s) 160, 317, 418, 795
FalI AAGNNNNNCTT 4 cut(s) 108, 140, 215, 247
FaqI GGGAC 3 cut(s) 682, 755, 991
FatI CATG 4 cut(s) 156, 313, 414, 791
FbaI TGATCA 2 cut(s) 112, 268
Fnu4HI GCNGC 4 cut(s) 251, 341, 463, 754
FokI GGATG 3 cut(s) 258, 900, 986
FriOI GRGCYC 1 cut(s) 832
Fsp4HI GCNGC 4 cut(s) 251, 341, 463, 754
FspBI CTAG 2 cut(s) 264, 647
GlaI GCGC 2 cut(s) 109, 344
GluI GCNGC 4 cut(s) 251, 341, 463, 754
GsuI CTGGAG 1 cut(s) 603
HaeII RGCGCY 1 cut(s) 111
HaeIII GGCC 3 cut(s) 227, 465, 558
HapII CCGG 3 cut(s) 9, 885, 1014
HgaI GACGC 1 cut(s) 161
HhaI GCGC 2 cut(s) 110, 345
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 4 cut(s) 160, 317, 418, 795
Hin6I GCGC 2 cut(s) 108, 343
HinP1I GCGC 2 cut(s) 108, 343
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HinfI GANTC 7 cut(s) 20, 625, 686, 740, 800, 1034, 1048
HpaII CCGG 3 cut(s) 9, 885, 1014
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 3 cut(s) 547, 717, 943
Hpy188I TCNGA 8 cut(s) 30, 423, 485, 630, 799, 805, 1031, 1064
Hpy188III TCNNGA 7 cut(s) 70, 355, 620, 838, 885, 913, 989
Hpy8I GTNNAC 3 cut(s) 547, 717, 943
HpyAV CCTTC 3 cut(s) 652, 733, 1015
HpyCH4III ACNGT 1 cut(s) 259
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 6 cut(s) 89, 447, 692, 756, 891, 903
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 349
HpyF3I CTNAG 5 cut(s) 482, 591, 654, 802, 946
HpySE526I ACGT 1 cut(s) 540
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 4 cut(s) 160, 317, 418, 795
HspAI GCGC 2 cut(s) 108, 343
KflI GGGWCCC 1 cut(s) 696
Kpn2I TCCGGA 1 cut(s) 884
Ksp22I TGATCA 2 cut(s) 112, 268
Kzo9I GATC 5 cut(s) 112, 189, 268, 511, 985
LmnI GCTCC 3 cut(s) 300, 622, 637
Lsp1109I GCAGC 3 cut(s) 237, 327, 740
LweI GCATC 1 cut(s) 878
MaeI CTAG 2 cut(s) 264, 647
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 2 cut(s) 259, 842
MalI GATC 5 cut(s) 114, 191, 270, 513, 987
MbiI CCGCTC 1 cut(s) 352
MboI GATC 5 cut(s) 112, 189, 268, 511, 985
MboII GAAGA 6 cut(s) 17, 116, 596, 826, 846, 1034
MfeI CAATTG 2 cut(s) 898, 981
MflI RGATCY 2 cut(s) 189, 511
MhlI GDGCHC 2 cut(s) 601, 832
MlyI GAGTC 1 cut(s) 794
MmeI TCCRAC 2 cut(s) 452, 822
MroI TCCGGA 1 cut(s) 884
MroXI GAANNNNTTC 1 cut(s) 101
MseI TTAA 3 cut(s) 288, 666, 963
MslI CAYNNNNRTG 2 cut(s) 89, 606
MspI CCGG 3 cut(s) 9, 885, 1014
MspR9I CCNGG 5 cut(s) 10, 242, 333, 453, 1015
MunI CAATTG 2 cut(s) 898, 981
MvaI CCWGG 3 cut(s) 242, 333, 453
MwoI GCNNNNNNNGC 2 cut(s) 233, 349
NciI CCSGG 2 cut(s) 10, 1015
NcoI CCATGG 1 cut(s) 156
NdeII GATC 5 cut(s) 112, 189, 268, 511, 985
NlaIII CATG 4 cut(s) 160, 317, 418, 795
NlaIV GGNNCC 3 cut(s) 697, 698, 771
NmeAIII GCCGAG 1 cut(s) 27
OliI CACNNNNGTG 1 cut(s) 89
PaeR7I CTCGAG 1 cut(s) 353
PasI CCCWGGG 1 cut(s) 241
PceI AGGCCT 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 101
PfeI GAWTC 6 cut(s) 20, 625, 686, 740, 1034, 1048
PkrI GCNGC 4 cut(s) 252, 342, 464, 755
PleI GAGTC 1 cut(s) 794
PpsI GAGTC 1 cut(s) 794
PpuMI RGGWCCY 3 cut(s) 650, 696, 769
Psp5II RGGWCCY 3 cut(s) 650, 696, 769
Psp6I CCWGG 3 cut(s) 240, 331, 451
PspGI CCWGG 3 cut(s) 240, 331, 451
PspN4I GGNNCC 3 cut(s) 697, 698, 771
PspPI GGNCC 4 cut(s) 650, 696, 769, 795
PspPPI RGGWCCY 3 cut(s) 650, 696, 769
PsrI GAACNNNNNNTAC 2 cut(s) 835, 867
PstI CTGCAG 3 cut(s) 91, 694, 758
PsuI RGATCY 2 cut(s) 189, 511
RsaI GTAC 1 cut(s) 718
RsaNI GTAC 1 cut(s) 717
RseI CAYNNNNRTG 2 cut(s) 89, 606
SaqAI TTAA 3 cut(s) 288, 666, 963
SatI GCNGC 4 cut(s) 251, 341, 463, 754
Sau3AI GATC 5 cut(s) 112, 189, 268, 511, 985
Sau96I GGNCC 4 cut(s) 650, 696, 769, 795
SchI GAGTC 1 cut(s) 794
ScrFI CCNGG 5 cut(s) 10, 242, 333, 453, 1015
SduI GDGCHC 2 cut(s) 601, 832
SetI ASST 9 cut(s) 39, 187, 430, 472, 543, 652, 675, 771, 810
SfaNI GCATC 1 cut(s) 878
SfcI CTRYAG 3 cut(s) 87, 690, 754
Sfr274I CTCGAG 1 cut(s) 353
SinI GGWCC 4 cut(s) 650, 696, 769, 795
SlaI CTCGAG 1 cut(s) 353
SmiMI CAYNNNNRTG 2 cut(s) 89, 606
SmlI CTYRAG 2 cut(s) 353, 838
SmoI CTYRAG 2 cut(s) 353, 838
SpeI ACTAGT 1 cut(s) 263
SseBI AGGCCT 1 cut(s) 227
SsiI CCGC 3 cut(s) 94, 350, 462
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 264, 647
StuI AGGCCT 1 cut(s) 227
StyD4I CCNGG 5 cut(s) 8, 240, 331, 451, 1013
StyI CCWWGG 2 cut(s) 156, 466
TaaI ACNGT 1 cut(s) 259
TaiI ACGT 1 cut(s) 543
TaqI TCGA 2 cut(s) 354, 988
TatI WGTACW 1 cut(s) 716
TauI GCSGC 1 cut(s) 465
TfiI GAWTC 6 cut(s) 20, 625, 686, 740, 1034, 1048
Tru1I TTAA 3 cut(s) 288, 666, 963
Tru9I TTAA 3 cut(s) 288, 666, 963
TscAI CASTG 3 cut(s) 91, 96, 223
TseI GCWGC 3 cut(s) 250, 340, 753
TspDTI ATGAA 4 cut(s) 493, 753, 780, 846
TspRI CASTG 3 cut(s) 91, 96, 223
VpaK11BI GGWCC 4 cut(s) 650, 696, 769, 795
XapI RAATTY 3 cut(s) 284, 321, 814
XhoI CTCGAG 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 101
XspI CTAG 2 cut(s) 264, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.