MD02G1188500.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000513
Physical Location & Seq
Forward (+)
17225142 .. 17225721
580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1188500.v1.1.491

Sequence Viewer

Length: 300 bp
ATGGTGTGGATTGATTTTGGTCACCTATACCCTTACACTTTCATTCTCTCTATCCCGTGGCTCTCTCTCTCCTCCATCCCGACTCTCTCTCTACACACACCCATACGTACGGACACACCCAAAAATCTTCCAAACTCGACGGATCGAGGCAACCAAGGGAATTCTAAGCTTATAGTGAGCTTAGTGAGGTCCCAAGGAAGCTCGGAGTGCTTCGTTGGAAGGATTTGGACGTCGGGATCACGAGGGTTAAGTTTGGCCGATTTTGTTTGGATTTTTCCGGTGAGATTTCTTGATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

100

Amino Acids

11.17

Weight (kDa)

9.1

Isoelectric Point (pI)

23.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 233
AclWI GGATC 2 cut(s) 150, 244
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 230
AfaI GTAC 1 cut(s) 109
AluBI AGCT 3 cut(s) 169, 180, 201
AluI AGCT 3 cut(s) 169, 180, 201
AlwI GGATC 2 cut(s) 150, 244
AoxI GGCC 1 cut(s) 255
ApoI RAATTY 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 189
AsuHPI GGTGA 2 cut(s) 14, 292
AvaII GGWCC 1 cut(s) 189
BauI CACGAG 1 cut(s) 240
BccI CCATC 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 189
BmgT120I GGNCC 1 cut(s) 189
BmiI GGNNCC 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 107
BsaHI GRCGYC 1 cut(s) 230
BsaJI CCNNGG 3 cut(s) 56, 154, 193
BsaWI WCCGGW 1 cut(s) 277
BseDI CCNNGG 3 cut(s) 56, 154, 193
BseGI GGATG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 61
BshFI GGCC 1 cut(s) 257
BsiSI CCGG 1 cut(s) 278
BsiWI CGTACG 1 cut(s) 107
BslFI GGGAC 1 cut(s) 175
BsmFI GGGAC 1 cut(s) 175
BsnI GGCC 1 cut(s) 257
Bsp143I GATC 2 cut(s) 142, 236
BspANI GGCC 1 cut(s) 257
BspLI GGNNCC 1 cut(s) 191
BspPI GGATC 2 cut(s) 150, 244
BssECI CCNNGG 3 cut(s) 56, 154, 193
BssMI GATC 2 cut(s) 142, 236
BssNI GRCGYC 1 cut(s) 230
BssSI CACGAG 1 cut(s) 240
BssT1I CCWWGG 2 cut(s) 154, 193
Bst2BI CACGAG 1 cut(s) 240
BstACI GRCGYC 1 cut(s) 230
BstBAI YACGTR 1 cut(s) 107
BstDEI CTNAG 2 cut(s) 165, 181
BstDSI CCRYGG 1 cut(s) 56
BstEII GGTNACC 1 cut(s) 20
BstF5I GGATG 1 cut(s) 75
BstKTI GATC 2 cut(s) 145, 239
BstMBI GATC 2 cut(s) 142, 236
BstMWI GCNNNNNNNGC 1 cut(s) 207
BstPI GGTNACC 1 cut(s) 20
BstSNI TACGTA 1 cut(s) 107
BsuRI GGCC 1 cut(s) 257
BtgI CCRYGG 1 cut(s) 56
BtsCI GGATG 1 cut(s) 75
Cfr13I GGNCC 1 cut(s) 189
Csp6I GTAC 1 cut(s) 108
CviJI RGCY 5 cut(s) 61, 169, 180, 201, 257
CviKI_1 RGCY 5 cut(s) 61, 169, 180, 201, 257
CviQI GTAC 1 cut(s) 108
DdeI CTNAG 2 cut(s) 165, 181
DpnI GATC 2 cut(s) 144, 238
DpnII GATC 2 cut(s) 142, 236
EaeI YGGCCR 1 cut(s) 255
Eco105I TACGTA 1 cut(s) 107
Eco130I CCWWGG 2 cut(s) 154, 193
Eco47I GGWCC 1 cut(s) 189
Eco91I GGTNACC 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 189
EcoO65I GGTNACC 1 cut(s) 20
EcoRI GAATTC 1 cut(s) 160
EcoT14I CCWWGG 2 cut(s) 154, 193
ErhI CCWWGG 2 cut(s) 154, 193
FaiI YATR 3 cut(s) 28, 104, 173
FaqI GGGAC 1 cut(s) 175
FokI GGATG 1 cut(s) 62
HaeIII GGCC 1 cut(s) 257
HapII CCGG 1 cut(s) 278
Hin1I GRCGYC 1 cut(s) 230
HindIII AAGCTT 1 cut(s) 167
HinfI GANTC 1 cut(s) 82
HpaII CCGG 1 cut(s) 278
HphI GGTGA 2 cut(s) 14, 292
Hpy188I TCNGA 1 cut(s) 205
Hpy188III TCNNGA 4 cut(s) 79, 234, 240, 290
Hpy99I CGWCG 2 cut(s) 142, 235
HpyAV CCTTC 1 cut(s) 213
HpyCH4IV ACGT 2 cut(s) 106, 230
HpyF10VI GCNNNNNNNGC 1 cut(s) 207
HpyF3I CTNAG 2 cut(s) 165, 181
HpySE526I ACGT 2 cut(s) 106, 230
Hsp92I GRCGYC 1 cut(s) 230
Kzo9I GATC 2 cut(s) 142, 236
LpnPI CCDG 1 cut(s) 291
MaeII ACGT 2 cut(s) 106, 230
MaeIII GTNAC 1 cut(s) 20
MalI GATC 2 cut(s) 144, 238
MboI GATC 2 cut(s) 142, 236
MboII GAAGA 1 cut(s) 119
MluCI AATT 1 cut(s) 160
MlyI GAGTC 1 cut(s) 76
MmeI TCCRAC 1 cut(s) 196
MnlI CCTC 4 cut(s) 82, 140, 180, 236
MseI TTAA 1 cut(s) 248
MspI CCGG 1 cut(s) 278
MwoI GCNNNNNNNGC 1 cut(s) 207
NdeII GATC 2 cut(s) 142, 236
NlaIV GGNNCC 1 cut(s) 191
NmuCI GTSAC 1 cut(s) 20
Pfl23II CGTACG 1 cut(s) 107
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Ppu21I YACGTR 1 cut(s) 107
PpuMI RGGWCCY 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 189
PspEI GGTNACC 1 cut(s) 20
PspLI CGTACG 1 cut(s) 107
PspN4I GGNNCC 1 cut(s) 191
PspPI GGNCC 1 cut(s) 189
PspPPI RGGWCCY 1 cut(s) 189
RsaI GTAC 1 cut(s) 109
RsaNI GTAC 1 cut(s) 108
SaqAI TTAA 1 cut(s) 248
Sau3AI GATC 2 cut(s) 142, 236
Sau96I GGNCC 1 cut(s) 189
SchI GAGTC 1 cut(s) 76
SetI ASST 7 cut(s) 27, 109, 171, 182, 191, 203, 233
SinI GGWCC 1 cut(s) 189
SnaBI TACGTA 1 cut(s) 107
Sse9I AATT 1 cut(s) 160
StyI CCWWGG 2 cut(s) 154, 193
TaiI ACGT 2 cut(s) 109, 233
TaqI TCGA 2 cut(s) 137, 145
TasI AATT 1 cut(s) 160
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TseFI GTSAC 1 cut(s) 20
Tsp45I GTSAC 1 cut(s) 20
TspDTI ATGAA 1 cut(s) 31
TspGWI ACGGA 2 cut(s) 125, 155
VpaK11BI GGWCC 1 cut(s) 189
XapI RAATTY 1 cut(s) 160
ZraI GACGTC 1 cut(s) 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.