pycom08g16160

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000513
Physical Location & Seq
Forward (+)
16034263 .. 16035966
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g16160.1

Sequence Viewer

Length: 303 bp
ATGCCCTGTACCCCCTACCCCGTGCCCAATCGAAGAAACCAAGCACATATCCATGCTCGTGAGGCTTGTAGGAGTCCAACCATACCCATTTCAGAATTTCAAGCTTGTAGGAAGCTTGGTGAGGTCCCTAGGAGGCTCGGGGTGGTTCGTTTGAAGGTTTTGGACGTCGGGATCACGAGTTTCGAGGTTGGCCGGAGTTGGGGTGATTTTCCAGTTGTTTCGCATATAGGTGAATCCTATCCCGAGGACGAGCGTGGTCACTCGAGGCAGGGGGGTTACGACCCTTCCACATACCAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.29

Weight (kDa)

8.57

Isoelectric Point (pI)

76.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 168
AclWI GGATC 1 cut(s) 179
AcoI YGGCCR 1 cut(s) 190
AcsI RAATTY 1 cut(s) 95
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 10
AfiI CCNNNNNNNGG 1 cut(s) 199
AgsI TTSAA 2 cut(s) 101, 154
AluBI AGCT 2 cut(s) 104, 115
AluI AGCT 2 cut(s) 104, 115
AlwI GGATC 1 cut(s) 179
Ama87I CYCGRG 3 cut(s) 137, 242, 262
AoxI GGCC 1 cut(s) 190
ApoI RAATTY 1 cut(s) 95
AspA2I CCTAGG 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 124
AsuHPI GGTGA 3 cut(s) 131, 215, 242
AvaI CYCGRG 3 cut(s) 137, 242, 262
AvaII GGWCC 1 cut(s) 124
AvrII CCTAGG 1 cut(s) 128
BaeGI GKGCMC 1 cut(s) 27
BauI CACGAG 2 cut(s) 57, 175
BfaI CTAG 2 cut(s) 129, 301
BlnI CCTAGG 1 cut(s) 128
Bme18I GGWCC 1 cut(s) 124
BmeT110I CYCGRG 3 cut(s) 137, 242, 262
BmgT120I GGNCC 1 cut(s) 124
BmiI GGNNCC 1 cut(s) 126
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 2 cut(s) 128, 243
BsaXI ACNNNNNCTCC 2 cut(s) 187, 217
Bsc4I CCNNNNNNNGG 1 cut(s) 199
Bse1I ACTGG 2 cut(s) 212, 295
BseDI CCNNGG 2 cut(s) 128, 243
BseLI CCNNNNNNNGG 1 cut(s) 199
BseNI ACTGG 2 cut(s) 212, 295
BseSI GKGCMC 1 cut(s) 27
BshFI GGCC 1 cut(s) 192
BsiHKCI CYCGRG 3 cut(s) 137, 242, 262
BsiSI CCGG 1 cut(s) 193
BslFI GGGAC 1 cut(s) 110
BslI CCNNNNNNNGG 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 110
BsnI GGCC 1 cut(s) 192
BsoBI CYCGRG 3 cut(s) 137, 242, 262
Bsp1286I GDGCHC 1 cut(s) 27
Bsp143I GATC 1 cut(s) 171
BspANI GGCC 1 cut(s) 192
BspLI GGNNCC 1 cut(s) 126
BspPI GGATC 1 cut(s) 179
BsrI ACTGG 2 cut(s) 212, 295
BssECI CCNNGG 2 cut(s) 128, 243
BssMI GATC 1 cut(s) 171
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 2 cut(s) 57, 175
BssT1I CCWWGG 1 cut(s) 128
Bst2BI CACGAG 2 cut(s) 57, 175
BstACI GRCGYC 1 cut(s) 165
BstKTI GATC 1 cut(s) 174
BstMBI GATC 1 cut(s) 171
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstSLI GKGCMC 1 cut(s) 27
BsuRI GGCC 1 cut(s) 192
Cfr13I GGNCC 1 cut(s) 124
Csp6I GTAC 1 cut(s) 9
CviAII CATG 1 cut(s) 53
CviJI RGCY 5 cut(s) 65, 104, 115, 136, 192
CviKI_1 RGCY 5 cut(s) 65, 104, 115, 136, 192
CviQI GTAC 1 cut(s) 9
DpnI GATC 1 cut(s) 173
DpnII GATC 1 cut(s) 171
EaeI YGGCCR 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 128
Eco47I GGWCC 1 cut(s) 124
Eco88I CYCGRG 3 cut(s) 137, 242, 262
EcoO109I RGGNCCY 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 128
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 1 cut(s) 56
FaiI YATR 6 cut(s) 48, 54, 83, 225, 227, 292
FaqI GGGAC 1 cut(s) 110
FatI CATG 1 cut(s) 52
FspBI CTAG 2 cut(s) 129, 301
HaeIII GGCC 1 cut(s) 192
HapII CCGG 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 1 cut(s) 56
HindIII AAGCTT 2 cut(s) 102, 113
HinfI GANTC 2 cut(s) 73, 233
HpaII CCGG 1 cut(s) 193
HphI GGTGA 3 cut(s) 131, 215, 242
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 4 cut(s) 59, 169, 175, 242
Hpy99I CGWCG 1 cut(s) 170
HpyAV CCTTC 2 cut(s) 148, 294
HpyCH4IV ACGT 1 cut(s) 165
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpySE526I ACGT 1 cut(s) 165
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 1 cut(s) 56
Kzo9I GATC 1 cut(s) 171
LpnPI CCDG 4 cut(s) 19, 206, 225, 254
MaeI CTAG 2 cut(s) 129, 301
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 2 cut(s) 257, 275
MalI GATC 1 cut(s) 173
MboI GATC 1 cut(s) 171
MboII GAAGA 1 cut(s) 45
MhlI GDGCHC 1 cut(s) 27
MluCI AATT 1 cut(s) 95
MlyI GAGTC 1 cut(s) 82
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 6 cut(s) 55, 115, 126, 178, 238, 258
MslI CAYNNNNRTG 3 cut(s) 51, 57, 228
MspI CCGG 1 cut(s) 193
MwoI GCNNNNNNNGC 1 cut(s) 62
NdeII GATC 1 cut(s) 171
NlaIII CATG 1 cut(s) 56
NlaIV GGNNCC 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 257
PaeR7I CTCGAG 1 cut(s) 262
PfeI GAWTC 1 cut(s) 233
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
PpuMI RGGWCCY 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 124
PspN4I GGNNCC 1 cut(s) 126
PspPI GGNCC 1 cut(s) 124
PspPPI RGGWCCY 1 cut(s) 124
PspXI VCTCGAGB 1 cut(s) 262
RsaI GTAC 1 cut(s) 10
RsaNI GTAC 1 cut(s) 9
RseI CAYNNNNRTG 3 cut(s) 51, 57, 228
Sau3AI GATC 1 cut(s) 171
Sau96I GGNCC 1 cut(s) 124
SchI GAGTC 1 cut(s) 82
SduI GDGCHC 1 cut(s) 27
SetI ASST 7 cut(s) 106, 117, 126, 159, 168, 189, 232
Sfr274I CTCGAG 1 cut(s) 262
SinI GGWCC 1 cut(s) 124
SlaI CTCGAG 1 cut(s) 262
SmiMI CAYNNNNRTG 3 cut(s) 51, 57, 228
SmlI CTYRAG 1 cut(s) 262
SmoI CTYRAG 1 cut(s) 262
Sse9I AATT 1 cut(s) 95
SspMI CTAG 2 cut(s) 129, 301
StyI CCWWGG 1 cut(s) 128
TaiI ACGT 1 cut(s) 168
TaqI TCGA 3 cut(s) 31, 183, 263
TasI AATT 1 cut(s) 95
TfiI GAWTC 1 cut(s) 233
TseFI GTSAC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 257
VpaK11BI GGWCC 1 cut(s) 124
XapI RAATTY 1 cut(s) 95
XhoI CTCGAG 1 cut(s) 262
XmaJI CCTAGG 1 cut(s) 128
XspI CTAG 2 cut(s) 129, 301
ZraI GACGTC 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.