pycom01g21320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000513
Physical Location & Seq
Forward (+)
19424394 .. 19425912
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g21320.2

Sequence Viewer

Length: 1140 bp
ATGAGTTATGAAAACAATGAATATGATAGTTTGTTGTTAACTAAAAAAGGAAAAAAAATTAAAAGGGAGATCGGTAAAATACCAACCGACCGTATCCTTTTTTTCAATTTGTTTATTTATTTTTCCATTGAATTGATTTTTCTTCATAAATTACTTTATTTAGATTTGAATACCTTTAGATTTAAATTTAGATTCACATATGTAGACATCGAAATTTTGGTGAAATGCATTAAAGTTTTGACATGCCAGGGCATACATGGCATGCGCCACGTATCACACAAATTGTACATTGCCCCTAGTTCATTTTTTTGTCACGTTGTGGGTGTTTTGTGTGGTGTGGCATGTGTTGGACCACACACACACACACTCACACACACAGTCACTCTCCCCTCCCTCTGTCTCTCTCCCCCGTGTCGCTCTCTTCCTTCCCGTTATCACCATACGTACGGACAACCACCAAAACCACCCAAACCATCGCAGATCGAGGAAACCAAGGACACCATTCAACTCGTGAGGTCGAGGCGAGCTCAGACATACCATTTTCAGGTGAGAAATACTTCGTTTTCACGTCGATTCAACAATGGCCGATTTGAGTACTCTCGTAGGTGTCTTAGTGAGGTCCTAAGGAGCGTCGGAGTAGCTCGTTGGACAAAATTGGACGTCGGGAAGGCTTGGTTCGAAGTTGGCCGAGTTTTGAGTTTTGAAACAGGTATGATCGCGTATTTAGGGCTTTCTTTTGGTCCAAGAATCATAGAAAATGTTTTCCGGCGACCGGAGTCCGGCGAGAGTAGATCGGAGAAGAAAGGAGAATATTCCGTTAGGTTTAACGGAATATTCCTAACGGCAGTGACGGCGTCAGTTAGGTTTAACGGAATATTCCGTCAGTTTAACGGAATATTCCTGACGGCGTCTGTTGACACCGTCAGTGTGCAGGGCACGTGGCCGCGCGTGGGGGGCGAAAATTATTTTAAAAATATGGTTGTGATCTTGAAGTTGTGTATGAGAAGTTATTACGAGAAGTTAGTTATGTGCTTTAAAGTTAACGTTTTTATAGTTGTTTCGCATTTAGGTGACACGTACCCCGAGGACGAGCGTGCACACGCGAGGCAGGGGGGCTACGACCCTTCTACATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

43.72

Weight (kDa)

9.66

Isoelectric Point (pI)

41.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 663
AccI GTMKAC 1 cut(s) 204
AccII CGCG 4 cut(s) 719, 946, 948, 1103
AciI CCGC 1 cut(s) 944
AclI AACGTT 1 cut(s) 1044
AcoI YGGCCR 3 cut(s) 583, 685, 941
AcsI RAATTY 2 cut(s) 185, 213
AcvI CACGTG 1 cut(s) 939
AcyI GRCGYC 3 cut(s) 660, 854, 908
AdeI CACNNNGTG 1 cut(s) 319
AfaI GTAC 4 cut(s) 287, 446, 596, 1079
AfiI CCNNNNNNNGG 4 cut(s) 544, 772, 779, 950
AflIII ACRYGT 1 cut(s) 1074
AgsI TTSAA 7 cut(s) 106, 131, 169, 506, 577, 704, 991
AjiI CACGTC 1 cut(s) 569
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 2 cut(s) 527, 641
AluI AGCT 2 cut(s) 527, 641
Alw21I GWGCWC 2 cut(s) 529, 1099
Alw26I GTCTC 1 cut(s) 404
Alw44I GTGCAC 1 cut(s) 1095
Ama87I CYCGRG 1 cut(s) 1082
AoxI GGCC 3 cut(s) 583, 685, 941
ApaLI GTGCAC 1 cut(s) 1095
ApoI RAATTY 2 cut(s) 185, 213
Asp700I GAANNNNTTC 1 cut(s) 556
AspLEI GCGC 2 cut(s) 267, 948
AspS9I GGNCC 3 cut(s) 350, 619, 740
AsuHPI GGTGA 4 cut(s) 232, 428, 559, 1082
AsuII TTCGAA 1 cut(s) 678
AvaI CYCGRG 1 cut(s) 1082
AvaII GGWCC 3 cut(s) 350, 619, 740
AxyI CCTNAGG 1 cut(s) 623
BaeGI GKGCMC 2 cut(s) 938, 1099
BanII GRGCYC 1 cut(s) 529
BauI CACGAG 1 cut(s) 509
BbrPI CACGTG 1 cut(s) 939
Bbv12I GWGCWC 2 cut(s) 529, 1099
BccI CCATC 1 cut(s) 481
BceAI ACGGC 3 cut(s) 858, 867, 921
BciT130I CCWGG 1 cut(s) 248
BciVI GTATCC 1 cut(s) 104
BcoDI GTCTC 1 cut(s) 404
BfaI CTAG 1 cut(s) 297
BfuI GTATCC 1 cut(s) 104
BisI GCNGC 1 cut(s) 944
BlsI GCNGC 1 cut(s) 945
BmcAI AGTACT 1 cut(s) 596
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 3 cut(s) 350, 619, 740
BmeT110I CYCGRG 1 cut(s) 1082
BmgBI CACGTC 1 cut(s) 569
BmgT120I GGNCC 3 cut(s) 350, 619, 740
BmrFI CCNGG 1 cut(s) 248
BoxI GACNNNNGTC 1 cut(s) 775
BplI GAGNNNNNCTC 2 cut(s) 511, 543
Bpu14I TTCGAA 1 cut(s) 678
BsaAI YACGTR 4 cut(s) 271, 444, 939, 1077
BsaHI GRCGYC 3 cut(s) 660, 854, 908
BsaJI CCNNGG 3 cut(s) 247, 492, 1083
BsaWI WCCGGW 1 cut(s) 772
Bsc4I CCNNNNNNNGG 4 cut(s) 544, 772, 779, 950
Bse1I ACTGG 1 cut(s) 1135
Bse21I CCTNAGG 1 cut(s) 623
Bse3DI GCAATG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 248
BseDI CCNNGG 3 cut(s) 247, 492, 1083
BseLI CCNNNNNNNGG 4 cut(s) 544, 772, 779, 950
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 1 cut(s) 1135
BseSI GKGCMC 2 cut(s) 938, 1099
BsgI GTGCAG 1 cut(s) 950
Bsh1236I CGCG 4 cut(s) 719, 946, 948, 1103
Bsh1285I CGRYCG 2 cut(s) 91, 773
BshFI GGCC 3 cut(s) 585, 687, 943
BsiEI CGRYCG 2 cut(s) 91, 773
BsiHKAI GWGCWC 2 cut(s) 529, 1099
BsiHKCI CYCGRG 1 cut(s) 1082
BsiSI CCGG 3 cut(s) 766, 773, 780
BsiWI CGTACG 1 cut(s) 444
BslI CCNNNNNNNGG 4 cut(s) 544, 772, 779, 950
BsmAI GTCTC 1 cut(s) 404
BsnI GGCC 3 cut(s) 585, 687, 943
BsoBI CYCGRG 1 cut(s) 1082
Bsp119I TTCGAA 1 cut(s) 678
Bsp1286I GDGCHC 3 cut(s) 529, 938, 1099
Bsp1407I TGTACA 1 cut(s) 285
Bsp143I GATC 5 cut(s) 69, 480, 714, 791, 984
BspACI CCGC 1 cut(s) 944
BspANI GGCC 3 cut(s) 585, 687, 943
BspCNI CTCAG 1 cut(s) 541
BspFNI CGCG 4 cut(s) 719, 946, 948, 1103
BspT104I TTCGAA 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 288
BsrGI TGTACA 1 cut(s) 285
BsrI ACTGG 1 cut(s) 1135
BssECI CCNNGG 3 cut(s) 247, 492, 1083
BssMI GATC 5 cut(s) 69, 480, 714, 791, 984
BssNI GRCGYC 3 cut(s) 660, 854, 908
BssSI CACGAG 1 cut(s) 509
BssT1I CCWWGG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 509
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 3 cut(s) 92, 379, 922
Bst6I CTCTTC 1 cut(s) 426
BstACI GRCGYC 3 cut(s) 660, 854, 908
BstAUI TGTACA 1 cut(s) 285
BstBAI YACGTR 4 cut(s) 271, 444, 939, 1077
BstBI TTCGAA 1 cut(s) 678
BstC8I GCNNGC 3 cut(s) 263, 525, 1095
BstDEI CTNAG 3 cut(s) 528, 611, 623
BstFNI CGCG 4 cut(s) 719, 946, 948, 1103
BstHHI GCGC 2 cut(s) 267, 948
BstKTI GATC 5 cut(s) 72, 483, 717, 794, 987
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 5 cut(s) 69, 480, 714, 791, 984
BstMCI CGRYCG 2 cut(s) 91, 773
BstMWI GCNNNNNNNGC 3 cut(s) 258, 851, 954
BstNI CCWGG 1 cut(s) 248
BstNSI RCATGY 3 cut(s) 246, 265, 345
BstPAI GACNNNNGTC 1 cut(s) 775
BstSCI CCNGG 1 cut(s) 246
BstSLI GKGCMC 2 cut(s) 938, 1099
BstSNI TACGTA 1 cut(s) 444
BstUI CGCG 4 cut(s) 719, 946, 948, 1103
Bsu36I CCTNAGG 1 cut(s) 623
BsuI GTATCC 1 cut(s) 104
BsuRI GGCC 3 cut(s) 585, 687, 943
BtgZI GCGATG 1 cut(s) 459
BtrI CACGTC 1 cut(s) 569
BtsI GCAGTG 1 cut(s) 852
BtsIMutI CAGTG 2 cut(s) 852, 931
Cac8I GCNNGC 3 cut(s) 263, 525, 1095
CfoI GCGC 2 cut(s) 267, 948
Cfr13I GGNCC 3 cut(s) 350, 619, 740
CseI GACGC 3 cut(s) 619, 843, 897
Csp6I GTAC 4 cut(s) 286, 445, 595, 1078
CviAII CATG 4 cut(s) 243, 257, 262, 342
CviJI RGCY 8 cut(s) 527, 585, 641, 671, 687, 730, 943, 1116
CviKI_1 RGCY 8 cut(s) 527, 585, 641, 671, 687, 730, 943, 1116
CviQI GTAC 4 cut(s) 286, 445, 595, 1078
DdeI CTNAG 3 cut(s) 528, 611, 623
DpnI GATC 5 cut(s) 71, 482, 716, 793, 986
DpnII GATC 5 cut(s) 69, 480, 714, 791, 984
DraI TTTAAA 3 cut(s) 184, 970, 1036
DraIII CACNNNGTG 1 cut(s) 319
EaeI YGGCCR 3 cut(s) 583, 685, 941
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
Ecl136II GAGCTC 1 cut(s) 527
Eco105I TACGTA 1 cut(s) 444
Eco130I CCWWGG 1 cut(s) 492
Eco24I GRGCYC 1 cut(s) 529
Eco47I GGWCC 3 cut(s) 350, 619, 740
Eco53kI GAGCTC 1 cut(s) 527
Eco72I CACGTG 1 cut(s) 939
Eco81I CCTNAGG 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 1082
EcoICRI GAGCTC 1 cut(s) 527
EcoO109I RGGNCCY 1 cut(s) 619
EcoRII CCWGG 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 492
EcoT22I ATGCAT 1 cut(s) 230
EcoT38I GRGCYC 1 cut(s) 529
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 4 cut(s) 246, 260, 265, 345
FatI CATG 4 cut(s) 242, 256, 261, 341
FauNDI CATATG 1 cut(s) 199
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 1 cut(s) 944
FriOI GRGCYC 1 cut(s) 529
Fsp4HI GCNGC 1 cut(s) 944
FspBI CTAG 1 cut(s) 297
GlaI GCGC 2 cut(s) 266, 947
GluI GCNGC 1 cut(s) 944
HaeIII GGCC 3 cut(s) 585, 687, 943
HapII CCGG 3 cut(s) 766, 773, 780
HgaI GACGC 3 cut(s) 619, 843, 897
HhaI GCGC 2 cut(s) 267, 948
Hin1I GRCGYC 3 cut(s) 660, 854, 908
Hin1II CATG 4 cut(s) 246, 260, 265, 345
Hin6I GCGC 2 cut(s) 265, 946
HinP1I GCGC 2 cut(s) 265, 946
HincII GTYRAC 3 cut(s) 39, 916, 1042
HindII GTYRAC 3 cut(s) 39, 916, 1042
HinfI GANTC 4 cut(s) 192, 573, 747, 776
HpaI GTTAAC 2 cut(s) 39, 1042
HpaII CCGG 3 cut(s) 766, 773, 780
HphI GGTGA 4 cut(s) 232, 428, 559, 1082
Hpy166II GTNNAC 5 cut(s) 39, 205, 916, 1042, 1097
Hpy188I TCNGA 3 cut(s) 531, 635, 796
Hpy188III TCNNGA 4 cut(s) 511, 664, 901, 988
Hpy8I GTNNAC 5 cut(s) 39, 205, 916, 1042, 1097
Hpy99I CGWCG 3 cut(s) 573, 635, 665
HpyAV CCTTC 3 cut(s) 435, 661, 1134
HpyCH4III ACNGT 3 cut(s) 92, 379, 922
HpyCH4IV ACGT 8 cut(s) 270, 315, 443, 568, 660, 938, 1044, 1076
HpyCH4V TGCA 3 cut(s) 228, 931, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 258, 851, 954
HpyF3I CTNAG 3 cut(s) 528, 611, 623
HpySE526I ACGT 8 cut(s) 270, 315, 443, 568, 660, 938, 1044, 1076
Hsp92I GRCGYC 3 cut(s) 660, 854, 908
Hsp92II CATG 4 cut(s) 246, 260, 265, 345
HspAI GCGC 2 cut(s) 265, 946
KspAI GTTAAC 2 cut(s) 39, 1042
Kzo9I GATC 5 cut(s) 69, 480, 714, 791, 984
LmnI GCTCC 1 cut(s) 627
MaeI CTAG 1 cut(s) 297
MaeII ACGT 8 cut(s) 270, 315, 443, 568, 660, 938, 1044, 1076
MaeIII GTNAC 4 cut(s) 311, 379, 847, 1070
MalI GATC 5 cut(s) 71, 482, 716, 793, 986
MboI GATC 5 cut(s) 69, 480, 714, 791, 984
MboII GAAGA 3 cut(s) 134, 413, 811
MhlI GDGCHC 3 cut(s) 529, 938, 1099
MluCI AATT 9 cut(s) 57, 106, 131, 149, 185, 213, 281, 653, 961
MlyI GAGTC 1 cut(s) 785
MmeI TCCRAC 3 cut(s) 328, 613, 626
MnlI CCTC 8 cut(s) 400, 404, 478, 507, 513, 610, 1078, 1098
Mph1103I ATGCAT 1 cut(s) 230
MroXI GAANNNNTTC 1 cut(s) 556
MslI CAYNNNNRTG 2 cut(s) 1068, 1135
MspI CCGG 3 cut(s) 766, 773, 780
MspR9I CCNGG 1 cut(s) 248
MvaI CCWGG 1 cut(s) 248
MvnI CGCG 4 cut(s) 719, 946, 948, 1103
MwoI GCNNNNNNNGC 3 cut(s) 258, 851, 954
NdeI CATATG 1 cut(s) 199
NdeII GATC 5 cut(s) 69, 480, 714, 791, 984
NlaIII CATG 4 cut(s) 246, 260, 265, 345
NmeAIII GCCGAG 1 cut(s) 713
NmuCI GTSAC 4 cut(s) 311, 379, 847, 1070
NsiI ATGCAT 1 cut(s) 230
NspI RCATGY 3 cut(s) 246, 265, 345
NspV TTCGAA 1 cut(s) 678
PaeI GCATGC 1 cut(s) 265
PdmI GAANNNNTTC 1 cut(s) 556
PfeI GAWTC 3 cut(s) 192, 573, 747
Pfl23II CGTACG 1 cut(s) 444
PflFI GACNNNGTC 3 cut(s) 853, 907, 920
PkrI GCNGC 1 cut(s) 945
PleI GAGTC 1 cut(s) 784
PmaCI CACGTG 1 cut(s) 939
PmlI CACGTG 1 cut(s) 939
PpsI GAGTC 1 cut(s) 784
Ppu21I YACGTR 4 cut(s) 271, 444, 939, 1077
PpuMI RGGWCCY 1 cut(s) 619
PshAI GACNNNNGTC 1 cut(s) 775
Psp124BI GAGCTC 1 cut(s) 529
Psp1406I AACGTT 1 cut(s) 1044
Psp5II RGGWCCY 1 cut(s) 619
Psp6I CCWGG 1 cut(s) 246
PspCI CACGTG 1 cut(s) 939
PspGI CCWGG 1 cut(s) 246
PspLI CGTACG 1 cut(s) 444
PspPI GGNCC 3 cut(s) 350, 619, 740
PspPPI RGGWCCY 1 cut(s) 619
PsyI GACNNNGTC 3 cut(s) 853, 907, 920
RsaI GTAC 4 cut(s) 287, 446, 596, 1079
RsaNI GTAC 4 cut(s) 286, 445, 595, 1078
RseI CAYNNNNRTG 2 cut(s) 1068, 1135
SacI GAGCTC 1 cut(s) 529
SatI GCNGC 1 cut(s) 944
Sau3AI GATC 5 cut(s) 69, 480, 714, 791, 984
Sau96I GGNCC 3 cut(s) 350, 619, 740
ScaI AGTACT 1 cut(s) 596
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 3 cut(s) 529, 938, 1099
SfuI TTCGAA 1 cut(s) 678
SinI GGWCC 3 cut(s) 350, 619, 740
SmiI ATTTAAAT 1 cut(s) 184
SmiMI CAYNNNNRTG 2 cut(s) 1068, 1135
SnaBI TACGTA 1 cut(s) 444
SphI GCATGC 1 cut(s) 265
Sse9I AATT 9 cut(s) 57, 106, 131, 149, 185, 213, 281, 653, 961
SsiI CCGC 1 cut(s) 944
SspI AATATT 4 cut(s) 812, 834, 876, 897
SspMI CTAG 1 cut(s) 297
SstI GAGCTC 1 cut(s) 529
StyD4I CCNGG 1 cut(s) 246
StyI CCWWGG 1 cut(s) 492
SwaI ATTTAAAT 1 cut(s) 184
TaaI ACNGT 3 cut(s) 92, 379, 922
TaiI ACGT 8 cut(s) 273, 318, 446, 571, 663, 941, 1047, 1079
TaqI TCGA 5 cut(s) 210, 483, 518, 571, 678
TasI AATT 9 cut(s) 57, 106, 131, 149, 185, 213, 281, 653, 961
TatI WGTACW 2 cut(s) 285, 594
TauI GCSGC 1 cut(s) 946
TfiI GAWTC 3 cut(s) 192, 573, 747
TscAI CASTG 2 cut(s) 852, 931
TseFI GTSAC 4 cut(s) 311, 379, 847, 1070
Tsp45I GTSAC 4 cut(s) 311, 379, 847, 1070
TspDTI ATGAA 4 cut(s) 24, 33, 134, 291
TspGWI ACGGA 6 cut(s) 462, 805, 843, 869, 885, 906
TspRI CASTG 2 cut(s) 852, 931
Tth111I GACNNNGTC 3 cut(s) 853, 907, 920
VneI GTGCAC 1 cut(s) 1095
VpaK11BI GGWCC 3 cut(s) 350, 619, 740
XapI RAATTY 2 cut(s) 185, 213
XceI RCATGY 3 cut(s) 246, 265, 345
XcmI CCANNNNNNNNNTGG 1 cut(s) 254
XmiI GTMKAC 1 cut(s) 204
XmnI GAANNNNTTC 1 cut(s) 556
XspI CTAG 1 cut(s) 297
ZraI GACGTC 1 cut(s) 661
ZrmI AGTACT 1 cut(s) 596
Zsp2I ATGCAT 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.