pycom07g12550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000513
Physical Location & Seq
Forward (+)
13884249 .. 13885321
1073 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g12550.1

Sequence Viewer

Length: 672 bp
ATGCGAGCGCTAGAGAATCATAGCGTGGACTCCAGTTCTATCGACACTTTTAAATTGATTAGGCATATTACTTTCATATATAATTATTGTGAATGCCTAAAATGCAGCCATAAAATATTTGAGACAAAAGCCTTATTTGGGAAATTGAGTAATGCGAAGGAAATTGATAAAGACAAGTTTTACGTTATTGATTGTACATTTCACACCGTACTGTTTATAATTGAAAATGCTACAACATGGTTGAGTTTTAGATTGCATCATGGTATATCCATCTGTATACTACTTGCATATAATTATTGGGAATGCTTTCAATTGCAAAGGTGGACGCAGGACACCCAGGTAAGCTTCAGAAACCTGCACCCCGGTGTTAGTGCTCCCGCCTGTGGCCAGGGCACAGTCCTTCACGTGATGTTCACCTCTCACACCTTACGCTCACCTTGGATTCAAGGTAAGTGCACAGTGACCCGCGATTATTTGCCCAGTCTTCAAGTGATCGTATGTGCACTTGAGCATATTTATTTACACCCAGTATACTTATTGAGCTATTGGCTAGCCAGGATTGATGAGATATGGATAAGTCGTACATTCGTACAGGTCACTATAGGTGACTCCGACTTATATGCTAGCAGGATTGATGAGATATGGATAAGTCATACAGGTCACTATAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

224

Amino Acids

26.07

Weight (kDa)

7.19

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 218
Acc36I ACCTGC 1 cut(s) 363
AccI GTMKAC 2 cut(s) 277, 531
AccII CGCG 1 cut(s) 468
AciI CCGC 2 cut(s) 378, 466
AcoI YGGCCR 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 331
AcvI CACGTG 1 cut(s) 406
AfaI GTAC 4 cut(s) 196, 210, 583, 591
AfeI AGCGCT 1 cut(s) 9
AfiI CCNNNNNNNGG 2 cut(s) 138, 383
AgsI TTSAA 4 cut(s) 224, 311, 446, 488
AjnI CCWGG 3 cut(s) 336, 387, 554
AleI CACNNNNGTG 1 cut(s) 363
AluBI AGCT 2 cut(s) 345, 543
AluI AGCT 2 cut(s) 345, 543
Alw21I GWGCWC 3 cut(s) 376, 458, 505
Alw26I GTCTC 1 cut(s) 116
Alw44I GTGCAC 2 cut(s) 454, 501
Aor51HI AGCGCT 1 cut(s) 9
AoxI GGCC 1 cut(s) 385
ApaLI GTGCAC 2 cut(s) 454, 501
ApeKI GCWGC 1 cut(s) 105
Asp700I GAANNNNTTC 1 cut(s) 306
AspLEI GCGC 1 cut(s) 10
AsuC2I CCSGG 1 cut(s) 363
AsuHPI GGTGA 3 cut(s) 406, 426, 617
AsuNHI GCTAGC 2 cut(s) 550, 623
BaeGI GKGCMC 3 cut(s) 395, 458, 505
BalI TGGCCA 1 cut(s) 387
BbrPI CACGTG 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 3 cut(s) 376, 458, 505
BbvI GCAGC 1 cut(s) 117
BccI CCATC 1 cut(s) 278
BcgI CGANNNNNNTGC 2 cut(s) 602, 636
BciT130I CCWGG 3 cut(s) 338, 389, 556
BcnI CCSGG 1 cut(s) 363
BcoDI GTCTC 1 cut(s) 116
BfaI CTAG 3 cut(s) 11, 551, 624
BfmI CTRYAG 2 cut(s) 600, 664
BfoI RGCGCY 1 cut(s) 11
BfuAI ACCTGC 1 cut(s) 363
BisI GCNGC 1 cut(s) 106
BlsI GCNGC 1 cut(s) 107
Bme1390I CCNGG 4 cut(s) 338, 363, 389, 556
BmrFI CCNGG 4 cut(s) 338, 363, 389, 556
BmrI ACTGGG 2 cut(s) 474, 521
BmsI GCATC 1 cut(s) 265
BmtI GCTAGC 2 cut(s) 554, 627
BmuI ACTGGG 2 cut(s) 474, 521
BpiI GAAGAC 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 406
BsaJI CCNNGG 4 cut(s) 336, 361, 388, 437
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 383
Bse1I ACTGG 3 cut(s) 33, 480, 527
BseBI CCWGG 3 cut(s) 338, 389, 556
BseDI CCNNGG 4 cut(s) 336, 361, 388, 437
BseLI CCNNNNNNNGG 2 cut(s) 138, 383
BseNI ACTGG 3 cut(s) 33, 480, 527
BseSI GKGCMC 3 cut(s) 395, 458, 505
BseXI GCAGC 1 cut(s) 117
BsgI GTGCAG 1 cut(s) 341
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 1 cut(s) 387
BsiHKAI GWGCWC 3 cut(s) 376, 458, 505
BsiSI CCGG 1 cut(s) 363
BslI CCNNNNNNNGG 2 cut(s) 138, 383
BsmAI GTCTC 1 cut(s) 116
BsmI GAATGC 2 cut(s) 98, 308
BsnI GGCC 1 cut(s) 387
Bsp1286I GDGCHC 4 cut(s) 376, 395, 458, 505
Bsp1407I TGTACA 1 cut(s) 194
Bsp143I GATC 1 cut(s) 492
BspACI CCGC 2 cut(s) 378, 466
BspANI GGCC 1 cut(s) 387
BspFNI CGCG 1 cut(s) 468
BspMI ACCTGC 1 cut(s) 363
BspOI GCTAGC 2 cut(s) 554, 627
BsrGI TGTACA 1 cut(s) 194
BsrI ACTGG 3 cut(s) 33, 480, 527
BssECI CCNNGG 4 cut(s) 336, 361, 388, 437
BssMI GATC 1 cut(s) 492
BssNAI GTATAC 2 cut(s) 278, 532
BssT1I CCWWGG 1 cut(s) 437
Bst1107I GTATAC 2 cut(s) 278, 532
Bst2UI CCWGG 3 cut(s) 338, 389, 556
Bst4CI ACNGT 4 cut(s) 208, 213, 397, 460
BstAUI TGTACA 1 cut(s) 194
BstBAI YACGTR 1 cut(s) 406
BstC8I GCNNGC 3 cut(s) 6, 552, 625
BstFNI CGCG 1 cut(s) 468
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 1 cut(s) 495
BstMAI GTCTC 1 cut(s) 116
BstMBI GATC 1 cut(s) 492
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNI CCWGG 3 cut(s) 338, 389, 556
BstSCI CCNGG 4 cut(s) 336, 361, 387, 554
BstSFI CTRYAG 2 cut(s) 600, 664
BstSLI GKGCMC 3 cut(s) 395, 458, 505
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 1 cut(s) 117
BstV2I GAAGAC 1 cut(s) 476
BstZ17I GTATAC 2 cut(s) 278, 532
BsuRI GGCC 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 465
BveI ACCTGC 1 cut(s) 363
Cac8I GCNNGC 3 cut(s) 6, 552, 625
CfoI GCGC 1 cut(s) 10
CseI GACGC 1 cut(s) 334
Csp6I GTAC 4 cut(s) 195, 209, 582, 590
CviAII CATG 2 cut(s) 237, 260
CviJI RGCY 7 cut(s) 108, 131, 345, 387, 543, 550, 554
CviKI_1 RGCY 7 cut(s) 108, 131, 345, 387, 543, 550, 554
CviQI GTAC 4 cut(s) 195, 209, 582, 590
DpnI GATC 1 cut(s) 494
DpnII GATC 1 cut(s) 492
DraI TTTAAA 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 385
Eco130I CCWWGG 1 cut(s) 437
Eco47III AGCGCT 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 331
Eco72I CACGTG 1 cut(s) 406
EcoRII CCWGG 3 cut(s) 336, 387, 554
EcoT14I CCWWGG 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 437
FaeI CATG 2 cut(s) 240, 263
FatI CATG 2 cut(s) 236, 259
FauI CCCGC 2 cut(s) 385, 473
FblI GTMKAC 2 cut(s) 277, 531
Fnu4HI GCNGC 1 cut(s) 106
Fsp4HI GCNGC 1 cut(s) 106
FspBI CTAG 3 cut(s) 11, 551, 624
GlaI GCGC 1 cut(s) 9
GluI GCNGC 1 cut(s) 106
GsuI CTGGAG 1 cut(s) 16
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 1 cut(s) 387
HapII CCGG 1 cut(s) 363
HgaI GACGC 1 cut(s) 334
HhaI GCGC 1 cut(s) 10
Hin1II CATG 2 cut(s) 240, 263
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HindIII AAGCTT 1 cut(s) 343
HinfI GANTC 4 cut(s) 16, 29, 442, 608
HpaII CCGG 1 cut(s) 363
HphI GGTGA 3 cut(s) 406, 426, 617
Hpy166II GTNNAC 7 cut(s) 28, 278, 324, 414, 456, 503, 532
Hpy188I TCNGA 2 cut(s) 350, 613
Hpy8I GTNNAC 7 cut(s) 28, 278, 324, 414, 456, 503, 532
HpyAV CCTTC 2 cut(s) 151, 410
HpyCH4III ACNGT 4 cut(s) 208, 213, 397, 460
HpyCH4IV ACGT 2 cut(s) 183, 405
HpyCH4V TGCA 7 cut(s) 105, 256, 287, 316, 358, 456, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpySE526I ACGT 2 cut(s) 183, 405
Hsp92II CATG 2 cut(s) 240, 263
HspAI GCGC 1 cut(s) 8
Kzo9I GATC 1 cut(s) 492
LmnI GCTCC 1 cut(s) 379
Lsp1109I GCAGC 1 cut(s) 117
LweI GCATC 1 cut(s) 265
MaeI CTAG 3 cut(s) 11, 551, 624
MaeII ACGT 2 cut(s) 183, 405
MaeIII GTNAC 4 cut(s) 460, 595, 605, 659
MalI GATC 1 cut(s) 494
MboI GATC 1 cut(s) 492
MboII GAAGA 1 cut(s) 476
MfeI CAATTG 1 cut(s) 311
MhlI GDGCHC 4 cut(s) 376, 395, 458, 505
MlsI TGGCCA 1 cut(s) 387
MluCI AATT 7 cut(s) 53, 82, 143, 162, 219, 292, 311
MluNI TGGCCA 1 cut(s) 387
MlyI GAGTC 2 cut(s) 23, 602
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 1 cut(s) 427
Mox20I TGGCCA 1 cut(s) 387
MroXI GAANNNNTTC 1 cut(s) 306
MscI TGGCCA 1 cut(s) 387
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 1 cut(s) 363
Msp20I TGGCCA 1 cut(s) 387
MspI CCGG 1 cut(s) 363
MspR9I CCNGG 4 cut(s) 338, 363, 389, 556
MunI CAATTG 1 cut(s) 311
Mva1269I GAATGC 2 cut(s) 98, 308
MvaI CCWGG 3 cut(s) 338, 389, 556
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 1 cut(s) 102
NciI CCSGG 1 cut(s) 363
NdeII GATC 1 cut(s) 492
NheI GCTAGC 2 cut(s) 550, 623
NlaIII CATG 2 cut(s) 240, 263
NmuCI GTSAC 4 cut(s) 460, 595, 605, 659
OliI CACNNNNGTG 1 cut(s) 363
PctI GAATGC 2 cut(s) 98, 308
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 16, 442
PkrI GCNGC 1 cut(s) 107
PleI GAGTC 2 cut(s) 23, 602
PmaCI CACGTG 1 cut(s) 406
PmlI CACGTG 1 cut(s) 406
PpsI GAGTC 2 cut(s) 23, 602
Ppu21I YACGTR 1 cut(s) 406
PsiI TTATAA 1 cut(s) 218
Psp6I CCWGG 3 cut(s) 336, 387, 554
PspCI CACGTG 1 cut(s) 406
PspGI CCWGG 3 cut(s) 336, 387, 554
RsaI GTAC 4 cut(s) 196, 210, 583, 591
RsaNI GTAC 4 cut(s) 195, 209, 582, 590
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 1 cut(s) 106
Sau3AI GATC 1 cut(s) 492
SchI GAGTC 2 cut(s) 23, 602
ScrFI CCNGG 4 cut(s) 338, 363, 389, 556
SduI GDGCHC 4 cut(s) 376, 395, 458, 505
SfaNI GCATC 1 cut(s) 265
SfcI CTRYAG 2 cut(s) 600, 664
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 506
SmoI CTYRAG 1 cut(s) 506
Sse9I AATT 7 cut(s) 53, 82, 143, 162, 219, 292, 311
SsiI CCGC 2 cut(s) 378, 466
SspI AATATT 1 cut(s) 117
SspMI CTAG 3 cut(s) 11, 551, 624
StyD4I CCNGG 4 cut(s) 336, 361, 387, 554
StyI CCWWGG 1 cut(s) 437
TaaI ACNGT 4 cut(s) 208, 213, 397, 460
TaiI ACGT 2 cut(s) 186, 408
TaqI TCGA 1 cut(s) 42
TasI AATT 7 cut(s) 53, 82, 143, 162, 219, 292, 311
TatI WGTACW 1 cut(s) 194
TfiI GAWTC 2 cut(s) 16, 442
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 465
TseFI GTSAC 4 cut(s) 460, 595, 605, 659
TseI GCWGC 1 cut(s) 105
Tsp45I GTSAC 4 cut(s) 460, 595, 605, 659
TspDTI ATGAA 1 cut(s) 64
TspRI CASTG 1 cut(s) 465
VneI GTGCAC 2 cut(s) 454, 501
XmiI GTMKAC 2 cut(s) 277, 531
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 3 cut(s) 11, 551, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.