pycom12661g00080

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00012661
OG0000513
Physical Location & Seq
Forward (+)
67954 .. 68914
961 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12661g00080.3

Sequence Viewer

Length: 429 bp
ATGTTTTGGGTTTTGTTGGCATGTTTTGAGCCACACACTCACTCCCACACTCCACACCCTTTTCTCCCTTTCCCTGTACCAGTCCCGAGTTCCCATTCCTTTCCCATTTTCCTTGAAACGTACGGACACACCCACACACTCATCAAACCTTCACAGATCGAAGAAACCAAGCACATATCCATGCTCGTGAGGCTCATAAGAGTCCAACCATACCCATTTCAGTTTGTTCAAGCTTGTAGGAAGCTTGGTGAGGTCCCTAGGAGGCTCGGGGTGGTTCGTTTGAAGGTTTTGGACGTCGGGATCACAAGTTTCGAGGTTGGCCGGAGTTGGGGTGATTTTTCAGTTGTTTCGCATATAGGTGATACCTATCCCGAGGACGAGCGTGGTCACTCGAGGCAGGGGGGCTACGACCCTTCCACATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

143

Amino Acids

16.27

Weight (kDa)

7.16

Isoelectric Point (pI)

55.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
AclWI GGATC 1 cut(s) 308
AcoI YGGCCR 1 cut(s) 319
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 2 cut(s) 78, 122
AfiI CCNNNNNNNGG 1 cut(s) 328
AgsI TTSAA 3 cut(s) 116, 230, 283
AluBI AGCT 2 cut(s) 233, 244
AluI AGCT 2 cut(s) 233, 244
AlwI GGATC 1 cut(s) 308
Ama87I CYCGRG 4 cut(s) 85, 266, 371, 391
AoxI GGCC 1 cut(s) 319
AspA2I CCTAGG 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 253
AsuHPI GGTGA 3 cut(s) 260, 344, 371
AvaI CYCGRG 4 cut(s) 85, 266, 371, 391
AvaII GGWCC 1 cut(s) 253
AvrII CCTAGG 1 cut(s) 257
BauI CACGAG 1 cut(s) 185
BfaI CTAG 1 cut(s) 258
BlnI CCTAGG 1 cut(s) 257
Bme18I GGWCC 1 cut(s) 253
BmeT110I CYCGRG 4 cut(s) 85, 266, 371, 391
BmgT120I GGNCC 1 cut(s) 253
BmiI GGNNCC 1 cut(s) 255
BsaBI GATNNNNATC 1 cut(s) 366
BsaHI GRCGYC 1 cut(s) 294
BsaJI CCNNGG 2 cut(s) 257, 372
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 316, 346
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 2 cut(s) 80, 424
Bse8I GATNNNNATC 1 cut(s) 366
BseDI CCNNGG 2 cut(s) 257, 372
BseJI GATNNNNATC 1 cut(s) 366
BseLI CCNNNNNNNGG 1 cut(s) 328
BseNI ACTGG 2 cut(s) 80, 424
BshFI GGCC 1 cut(s) 321
BsiHKCI CYCGRG 4 cut(s) 85, 266, 371, 391
BsiSI CCGG 1 cut(s) 322
BsiWI CGTACG 1 cut(s) 120
BslFI GGGAC 2 cut(s) 68, 239
BslI CCNNNNNNNGG 1 cut(s) 328
BsmFI GGGAC 2 cut(s) 68, 239
BsnI GGCC 1 cut(s) 321
BsoBI CYCGRG 4 cut(s) 85, 266, 371, 391
Bsp143I GATC 2 cut(s) 156, 300
BspANI GGCC 1 cut(s) 321
BspLI GGNNCC 1 cut(s) 255
BspPI GGATC 1 cut(s) 308
BsrI ACTGG 2 cut(s) 80, 424
BssECI CCNNGG 2 cut(s) 257, 372
BssMI GATC 2 cut(s) 156, 300
BssNI GRCGYC 1 cut(s) 294
BssSI CACGAG 1 cut(s) 185
BssT1I CCWWGG 1 cut(s) 257
Bst2BI CACGAG 1 cut(s) 185
BstACI GRCGYC 1 cut(s) 294
BstKTI GATC 2 cut(s) 159, 303
BstMBI GATC 2 cut(s) 156, 300
BstMWI GCNNNNNNNGC 1 cut(s) 190
BstNSI RCATGY 1 cut(s) 24
BsuRI GGCC 1 cut(s) 321
Cfr13I GGNCC 1 cut(s) 253
Csp6I GTAC 2 cut(s) 77, 121
CviAII CATG 2 cut(s) 21, 181
CviJI RGCY 7 cut(s) 31, 193, 233, 244, 265, 321, 405
CviKI_1 RGCY 7 cut(s) 31, 193, 233, 244, 265, 321, 405
CviQI GTAC 2 cut(s) 77, 121
DpnI GATC 2 cut(s) 158, 302
DpnII GATC 2 cut(s) 156, 300
EaeI YGGCCR 1 cut(s) 319
Eco130I CCWWGG 1 cut(s) 257
Eco47I GGWCC 1 cut(s) 253
Eco88I CYCGRG 4 cut(s) 85, 266, 371, 391
EcoO109I RGGNCCY 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 257
FaeI CATG 2 cut(s) 24, 184
FaiI YATR 8 cut(s) 22, 176, 182, 197, 211, 354, 356, 421
FaqI GGGAC 2 cut(s) 68, 239
FatI CATG 2 cut(s) 20, 180
FspBI CTAG 1 cut(s) 258
HaeIII GGCC 1 cut(s) 321
HapII CCGG 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 2 cut(s) 24, 184
HindIII AAGCTT 2 cut(s) 231, 242
HinfI GANTC 1 cut(s) 201
HpaII CCGG 1 cut(s) 322
HphI GGTGA 3 cut(s) 260, 344, 371
Hpy188III TCNNGA 4 cut(s) 85, 187, 298, 371
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 3 cut(s) 159, 277, 423
HpyCH4IV ACGT 2 cut(s) 119, 294
HpyF10VI GCNNNNNNNGC 1 cut(s) 190
HpySE526I ACGT 2 cut(s) 119, 294
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 2 cut(s) 24, 184
Kzo9I GATC 2 cut(s) 156, 300
LpnPI CCDG 4 cut(s) 87, 93, 335, 383
MaeI CTAG 1 cut(s) 258
MaeII ACGT 2 cut(s) 119, 294
MaeIII GTNAC 1 cut(s) 386
MalI GATC 2 cut(s) 158, 302
MboI GATC 2 cut(s) 156, 300
MboII GAAGA 1 cut(s) 173
MlyI GAGTC 1 cut(s) 210
MmeI TCCRAC 1 cut(s) 229
MnlI CCTC 6 cut(s) 183, 244, 255, 307, 367, 387
MslI CAYNNNNRTG 4 cut(s) 179, 185, 357, 424
MspI CCGG 1 cut(s) 322
MwoI GCNNNNNNNGC 1 cut(s) 190
NdeII GATC 2 cut(s) 156, 300
NlaIII CATG 2 cut(s) 24, 184
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 386
NspI RCATGY 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 391
Pfl23II CGTACG 1 cut(s) 120
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PpuMI RGGWCCY 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 253
PspLI CGTACG 1 cut(s) 120
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 253
PspPPI RGGWCCY 1 cut(s) 253
PspXI VCTCGAGB 1 cut(s) 391
RsaI GTAC 2 cut(s) 78, 122
RsaNI GTAC 2 cut(s) 77, 121
RseI CAYNNNNRTG 4 cut(s) 179, 185, 357, 424
Sau3AI GATC 2 cut(s) 156, 300
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 1 cut(s) 210
Sfr274I CTCGAG 1 cut(s) 391
SinI GGWCC 1 cut(s) 253
SlaI CTCGAG 1 cut(s) 391
SmiMI CAYNNNNRTG 4 cut(s) 179, 185, 357, 424
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
SspMI CTAG 1 cut(s) 258
StyI CCWWGG 1 cut(s) 257
TaiI ACGT 2 cut(s) 122, 297
TaqI TCGA 3 cut(s) 159, 312, 392
TseFI GTSAC 1 cut(s) 386
Tsp45I GTSAC 1 cut(s) 386
TspGWI ACGGA 1 cut(s) 138
VpaK11BI GGWCC 1 cut(s) 253
XceI RCATGY 1 cut(s) 24
XhoI CTCGAG 1 cut(s) 391
XmaJI CCTAGG 1 cut(s) 257
XspI CTAG 1 cut(s) 258
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.