pycom05g23760

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000513
Physical Location & Seq
Forward (+)
25535957 .. 25537272
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g23760.1

Sequence Viewer

Length: 1032 bp
ATGGTGTGGTGTGTAGGACCACACACACACTCATACACCTCGTTTCACTCTCCCGGGATTCCCTCCCTCTTTTCCCTCATTCCTGTCTCTTCTCTGTCTCTCCTTTCTTTCTTTCTCTCTCTCTCTCTCACTCTCCCCGAGTCCTCTCTTCTTCTTCCTTCTTCAACACACGGACATACACACAAACTTACTCAATACTTCACCAATCGAAAAACCAAGACTACCATCGTGTTCGTGGAGCTGAGAGGAGTCCAGTGGTGCCATTTTCAGGTAAGGAAAACACCATTTTCACGTCGATATCACGAGGTCCGATTTGAACACTGTTCATGCAAACCTAAACTAGTCCCAAGGAGCCTCGGAGTAGTTCGTTGGACAAATTTGGACGTCGGGAAGGCTAGGTTCAAAGTTGGCCGAACTTTGATCGTTGTTGCAGGTATGATCTTGGTTGAAAAACGAAGGAGAAAACAAGGTTTGAAAATTACCCAGTTTTCCGGCGCCGTCGCCGGCGCCGGAGGTTCGCCGGAGAAGAAAGGAGAATATTCCGTTAAGTCTAACGGAATATTCCTAACGGCAGTGACAGAATCCGTGCACGTGGGCCGCGCGTGGGGGCGCGTAGGTCCGTGGCTGTTCAGGCGCGTGGGGGCGCGTGCGTGGTCCAAAAATTTTTCTAAAAATATGGGTGTGATCCTGAGGTTGTGTAGATCACATTGGTATATTCATTTGTCCAATTTGAGCAATGTTTTCGTAACTTTGTCGCGTATAGGTGATTCGTTTTCCGAAGACGAGCCAGTGAGTGGGCTTTTGTTTTCCGTATATACCTATATACATATTAATTCCCAGAAATTAAATAGAAAAGGTTATGTGTTTTATGCCATGCATTATATGAATGTTGTTTACGCATCATTGCATGTATTATTTGTGACATACATACATATATACATGTGTATTTGGTGCTGTGGATGCACAGGTAAGTGCCAGGTAAGCGGTATTCAGGTGGTTATGCATTCCTGTTTATTATGCAATAGTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

344

Amino Acids

38.78

Weight (kDa)

9.99

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 387
Acc36I ACCTGC 1 cut(s) 422
AccB1I GGYRCC 3 cut(s) 258, 494, 506
AccB7I CCANNNNNTGG 1 cut(s) 794
AccII CGCG 6 cut(s) 600, 602, 612, 636, 646, 757
AciI CCGC 2 cut(s) 598, 984
AclWI GGATC 1 cut(s) 679
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 2 cut(s) 376, 661
AcvI CACGTG 1 cut(s) 592
AcyI GRCGYC 3 cut(s) 384, 495, 507
AfiI CCNNNNNNNGG 3 cut(s) 268, 604, 794
AflIII ACRYGT 1 cut(s) 939
AgsI TTSAA 5 cut(s) 165, 317, 403, 449, 475
AhlI ACTAGT 1 cut(s) 340
AjiI CACGTC 1 cut(s) 293
AjnI CCWGG 1 cut(s) 975
AluBI AGCT 1 cut(s) 241
AluI AGCT 1 cut(s) 241
Alw21I GWGCWC 1 cut(s) 591
Alw26I GTCTC 2 cut(s) 91, 102
Alw44I GTGCAC 1 cut(s) 587
AlwI GGATC 1 cut(s) 679
Ama87I CYCGRG 2 cut(s) 53, 137
AoxI GGCC 2 cut(s) 409, 595
ApaLI GTGCAC 1 cut(s) 587
ApoI RAATTY 2 cut(s) 376, 661
AseI ATTAAT 1 cut(s) 831
AspLEI GCGC 6 cut(s) 497, 509, 602, 612, 636, 646
AspS9I GGNCC 5 cut(s) 17, 307, 595, 617, 654
AsuC2I CCSGG 2 cut(s) 54, 55
AsuHPI GGTGA 2 cut(s) 193, 776
AvaI CYCGRG 2 cut(s) 53, 137
AvaII GGWCC 4 cut(s) 17, 307, 617, 654
AxyI CCTNAGG 1 cut(s) 689
BaeGI GKGCMC 1 cut(s) 591
BanI GGYRCC 3 cut(s) 258, 494, 506
BauI CACGAG 1 cut(s) 302
BbrPI CACGTG 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 786
Bbv12I GWGCWC 1 cut(s) 591
BccI CCATC 1 cut(s) 233
BceAI ACGGC 2 cut(s) 482, 585
BcgI CGANNNNNNTGC 2 cut(s) 724, 758
BciT130I CCWGG 1 cut(s) 977
BcnI CCSGG 2 cut(s) 54, 55
BcoDI GTCTC 2 cut(s) 91, 102
BcuI ACTAGT 1 cut(s) 340
BfaI CTAG 2 cut(s) 341, 396
BfoI RGCGCY 2 cut(s) 498, 510
BfuAI ACCTGC 1 cut(s) 422
BisI GCNGC 1 cut(s) 598
BlsI GCNGC 1 cut(s) 599
Bme1390I CCNGG 3 cut(s) 54, 55, 977
Bme18I GGWCC 4 cut(s) 17, 307, 617, 654
BmeT110I CYCGRG 2 cut(s) 53, 137
BmgBI CACGTC 1 cut(s) 293
BmgT120I GGNCC 5 cut(s) 17, 307, 595, 617, 654
BmiI GGNNCC 4 cut(s) 260, 353, 496, 508
BmrFI CCNGG 3 cut(s) 54, 55, 977
BmrI ACTGGG 1 cut(s) 478
BmsI GCATC 2 cut(s) 908, 950
BmuI ACTGGG 1 cut(s) 478
BpiI GAAGAC 1 cut(s) 786
BpuMI CCSGG 2 cut(s) 54, 55
BsaAI YACGTR 1 cut(s) 592
BsaHI GRCGYC 3 cut(s) 384, 495, 507
BsaJI CCNNGG 4 cut(s) 53, 347, 355, 620
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 604, 794
Bse118I RCCGGY 1 cut(s) 503
Bse1I ACTGG 3 cut(s) 253, 484, 788
Bse21I CCTNAGG 1 cut(s) 689
Bse3DI GCAATG 2 cut(s) 742, 902
BseBI CCWGG 1 cut(s) 977
BseDI CCNNGG 4 cut(s) 53, 347, 355, 620
BseGI GGATG 1 cut(s) 965
BseLI CCNNNNNNNGG 3 cut(s) 268, 604, 794
BseMI GCAATG 2 cut(s) 742, 902
BseMII CTCAG 2 cut(s) 233, 680
BseNI ACTGG 3 cut(s) 253, 484, 788
BseRI GAGGAG 1 cut(s) 261
BseSI GKGCMC 1 cut(s) 591
Bsh1236I CGCG 6 cut(s) 600, 602, 612, 636, 646, 757
BshFI GGCC 2 cut(s) 411, 597
BshNI GGYRCC 3 cut(s) 258, 494, 506
BsiHKAI GWGCWC 1 cut(s) 591
BsiHKCI CYCGRG 2 cut(s) 53, 137
BsiSI CCGG 5 cut(s) 54, 492, 504, 510, 521
BslFI GGGAC 1 cut(s) 329
BslI CCNNNNNNNGG 3 cut(s) 268, 604, 794
BsmAI GTCTC 2 cut(s) 91, 102
BsmFI GGGAC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 1003
BsnI GGCC 2 cut(s) 411, 597
BsoBI CYCGRG 2 cut(s) 53, 137
Bsp1286I GDGCHC 1 cut(s) 591
Bsp143I GATC 4 cut(s) 420, 438, 684, 701
BspACI CCGC 2 cut(s) 598, 984
BspANI GGCC 2 cut(s) 411, 597
BspCNI CTCAG 2 cut(s) 234, 681
BspFNI CGCG 6 cut(s) 600, 602, 612, 636, 646, 757
BspLI GGNNCC 4 cut(s) 260, 353, 496, 508
BspMI ACCTGC 1 cut(s) 422
BspPI GGATC 1 cut(s) 679
BspT107I GGYRCC 3 cut(s) 258, 494, 506
BsrDI GCAATG 2 cut(s) 742, 902
BsrFI RCCGGY 1 cut(s) 503
BsrI ACTGG 3 cut(s) 253, 484, 788
BssAI RCCGGY 1 cut(s) 503
BssECI CCNNGG 4 cut(s) 53, 347, 355, 620
BssMI GATC 4 cut(s) 420, 438, 684, 701
BssNI GRCGYC 3 cut(s) 384, 495, 507
BssSI CACGAG 1 cut(s) 302
BssT1I CCWWGG 1 cut(s) 347
Bst2BI CACGAG 1 cut(s) 302
Bst2UI CCWGG 1 cut(s) 977
Bst4CI ACNGT 1 cut(s) 323
Bst6I CTCTTC 2 cut(s) 94, 153
BstACI GRCGYC 3 cut(s) 384, 495, 507
BstBAI YACGTR 1 cut(s) 592
BstC8I GCNNGC 2 cut(s) 505, 648
BstDEI CTNAG 2 cut(s) 242, 689
BstDSI CCRYGG 1 cut(s) 620
BstF5I GGATG 1 cut(s) 965
BstFNI CGCG 6 cut(s) 600, 602, 612, 636, 646, 757
BstH2I RGCGCY 2 cut(s) 498, 510
BstHHI GCGC 6 cut(s) 497, 509, 602, 612, 636, 646
BstKTI GATC 4 cut(s) 423, 441, 687, 704
BstMAI GTCTC 2 cut(s) 91, 102
BstMBI GATC 4 cut(s) 420, 438, 684, 701
BstMWI GCNNNNNNNGC 3 cut(s) 631, 960, 981
BstNI CCWGG 1 cut(s) 977
BstNSI RCATGY 2 cut(s) 911, 943
BstSCI CCNGG 3 cut(s) 52, 53, 975
BstSLI GKGCMC 1 cut(s) 591
BstUI CGCG 6 cut(s) 600, 602, 612, 636, 646, 757
BstV2I GAAGAC 1 cut(s) 786
Bsu36I CCTNAGG 1 cut(s) 689
BsuRI GGCC 2 cut(s) 411, 597
BtgI CCRYGG 1 cut(s) 620
BtrI CACGTC 1 cut(s) 293
BtsCI GGATG 1 cut(s) 965
BtsI GCAGTG 1 cut(s) 579
BtsIMutI CAGTG 4 cut(s) 260, 319, 579, 795
BveI ACCTGC 1 cut(s) 422
Cac8I GCNNGC 2 cut(s) 505, 648
CfoI GCGC 6 cut(s) 497, 509, 602, 612, 636, 646
Cfr10I RCCGGY 1 cut(s) 503
Cfr13I GGNCC 5 cut(s) 17, 307, 595, 617, 654
Cfr9I CCCGGG 1 cut(s) 53
CviAII CATG 4 cut(s) 327, 874, 908, 940
CviJI RGCY 8 cut(s) 241, 354, 395, 411, 597, 625, 787, 799
CviKI_1 RGCY 8 cut(s) 241, 354, 395, 411, 597, 625, 787, 799
DdeI CTNAG 2 cut(s) 242, 689
DinI GGCGCC 2 cut(s) 496, 508
DpnI GATC 4 cut(s) 422, 440, 686, 703
DpnII GATC 4 cut(s) 420, 438, 684, 701
EaeI YGGCCR 1 cut(s) 409
Eam1104I CTCTTC 2 cut(s) 94, 153
EarI CTCTTC 2 cut(s) 94, 153
Eco130I CCWWGG 1 cut(s) 347
Eco32I GATATC 1 cut(s) 299
Eco47I GGWCC 4 cut(s) 17, 307, 617, 654
Eco72I CACGTG 1 cut(s) 592
Eco81I CCTNAGG 1 cut(s) 689
Eco88I CYCGRG 2 cut(s) 53, 137
EcoRII CCWGG 1 cut(s) 975
EcoRV GATATC 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 347
EcoT22I ATGCAT 2 cut(s) 879, 1005
EgeI GGCGCC 2 cut(s) 496, 508
EheI GGCGCC 2 cut(s) 496, 508
ErhI CCWWGG 1 cut(s) 347
FaeI CATG 4 cut(s) 330, 877, 911, 943
FaqI GGGAC 1 cut(s) 329
FatI CATG 4 cut(s) 326, 873, 907, 939
Fnu4HI GCNGC 1 cut(s) 598
FokI GGATG 1 cut(s) 972
Fsp4HI GCNGC 1 cut(s) 598
FspBI CTAG 2 cut(s) 341, 396
GlaI GCGC 6 cut(s) 496, 508, 601, 611, 635, 645
GluI GCNGC 1 cut(s) 598
HaeII RGCGCY 2 cut(s) 498, 510
HaeIII GGCC 2 cut(s) 411, 597
HapII CCGG 5 cut(s) 54, 492, 504, 510, 521
HhaI GCGC 6 cut(s) 497, 509, 602, 612, 636, 646
Hin1I GRCGYC 3 cut(s) 384, 495, 507
Hin1II CATG 4 cut(s) 330, 877, 911, 943
Hin6I GCGC 6 cut(s) 495, 507, 600, 610, 634, 644
HinP1I GCGC 6 cut(s) 495, 507, 600, 610, 634, 644
HinfI GANTC 5 cut(s) 58, 140, 249, 581, 767
HpaII CCGG 5 cut(s) 54, 492, 504, 510, 521
HphI GGTGA 2 cut(s) 193, 776
Hpy166II GTNNAC 2 cut(s) 589, 895
Hpy188I TCNGA 3 cut(s) 311, 359, 778
Hpy188III TCNNGA 3 cut(s) 302, 388, 688
Hpy8I GTNNAC 2 cut(s) 589, 895
Hpy99I CGWCG 3 cut(s) 297, 389, 503
HpyAV CCTTC 3 cut(s) 168, 385, 450
HpyCH4III ACNGT 1 cut(s) 323
HpyCH4IV ACGT 3 cut(s) 292, 384, 591
HpyCH4V TGCA 8 cut(s) 330, 431, 589, 877, 907, 963, 1003, 1020
HpyF10VI GCNNNNNNNGC 3 cut(s) 631, 960, 981
HpyF3I CTNAG 2 cut(s) 242, 689
HpySE526I ACGT 3 cut(s) 292, 384, 591
Hsp92I GRCGYC 3 cut(s) 384, 495, 507
Hsp92II CATG 4 cut(s) 330, 877, 911, 943
HspAI GCGC 6 cut(s) 495, 507, 600, 610, 634, 644
KasI GGCGCC 2 cut(s) 494, 506
KroI GCCGGC 1 cut(s) 503
KroNI GCCGGC 1 cut(s) 505
Kzo9I GATC 4 cut(s) 420, 438, 684, 701
LmnI GCTCC 2 cut(s) 238, 351
LweI GCATC 2 cut(s) 908, 950
MaeI CTAG 2 cut(s) 341, 396
MaeII ACGT 3 cut(s) 292, 384, 591
MaeIII GTNAC 3 cut(s) 574, 745, 919
MalI GATC 4 cut(s) 422, 440, 686, 703
MboI GATC 4 cut(s) 420, 438, 684, 701
MboII GAAGA 7 cut(s) 81, 140, 143, 146, 153, 538, 791
MhlI GDGCHC 1 cut(s) 591
MluCI AATT 6 cut(s) 376, 477, 661, 727, 832, 842
Mly113I GGCGCC 2 cut(s) 495, 507
MlyI GAGTC 2 cut(s) 149, 258
MmeI TCCRAC 1 cut(s) 350
Mph1103I ATGCAT 2 cut(s) 879, 1005
MreI CGCCGGCG 1 cut(s) 503
MroNI GCCGGC 1 cut(s) 503
MseI TTAA 3 cut(s) 546, 831, 845
MspI CCGG 5 cut(s) 54, 492, 504, 510, 521
MspR9I CCNGG 3 cut(s) 54, 55, 977
Mva1269I GAATGC 1 cut(s) 1003
MvaI CCWGG 1 cut(s) 977
MvnI CGCG 6 cut(s) 600, 602, 612, 636, 646, 757
MwoI GCNNNNNNNGC 3 cut(s) 631, 960, 981
NaeI GCCGGC 1 cut(s) 505
NarI GGCGCC 2 cut(s) 495, 507
NciI CCSGG 2 cut(s) 54, 55
NdeII GATC 4 cut(s) 420, 438, 684, 701
NgoMIV GCCGGC 1 cut(s) 503
NlaIII CATG 4 cut(s) 330, 877, 911, 943
NlaIV GGNNCC 4 cut(s) 260, 353, 496, 508
NmuCI GTSAC 2 cut(s) 574, 919
NsiI ATGCAT 2 cut(s) 879, 1005
NspI RCATGY 2 cut(s) 911, 943
PciI ACATGT 1 cut(s) 939
PctI GAATGC 1 cut(s) 1003
PdiI GCCGGC 1 cut(s) 505
PfeI GAWTC 3 cut(s) 58, 581, 767
PflMI CCANNNNNTGG 1 cut(s) 794
PkrI GCNGC 1 cut(s) 599
PleI GAGTC 2 cut(s) 148, 257
PluTI GGCGCC 2 cut(s) 498, 510
PmaCI CACGTG 1 cut(s) 592
PmlI CACGTG 1 cut(s) 592
PpsI GAGTC 2 cut(s) 148, 257
Ppu21I YACGTR 1 cut(s) 592
PscI ACATGT 1 cut(s) 939
PshBI ATTAAT 1 cut(s) 831
Psp6I CCWGG 1 cut(s) 975
PspCI CACGTG 1 cut(s) 592
PspGI CCWGG 1 cut(s) 975
PspN4I GGNNCC 4 cut(s) 260, 353, 496, 508
PspPI GGNCC 5 cut(s) 17, 307, 595, 617, 654
SaqAI TTAA 3 cut(s) 546, 831, 845
SatI GCNGC 1 cut(s) 598
Sau3AI GATC 4 cut(s) 420, 438, 684, 701
Sau96I GGNCC 5 cut(s) 17, 307, 595, 617, 654
SchI GAGTC 2 cut(s) 149, 258
ScrFI CCNGG 3 cut(s) 54, 55, 977
SduI GDGCHC 1 cut(s) 591
SfaNI GCATC 2 cut(s) 908, 950
SfoI GGCGCC 2 cut(s) 496, 508
SgrAI CRCCGGYG 1 cut(s) 503
SinI GGWCC 4 cut(s) 17, 307, 617, 654
SmaI CCCGGG 1 cut(s) 55
SpeI ACTAGT 1 cut(s) 340
Sse9I AATT 6 cut(s) 376, 477, 661, 727, 832, 842
SsiI CCGC 2 cut(s) 598, 984
SspDI GGCGCC 2 cut(s) 494, 506
SspI AATATT 2 cut(s) 539, 561
SspMI CTAG 2 cut(s) 341, 396
StyD4I CCNGG 3 cut(s) 52, 53, 975
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 1 cut(s) 323
TaiI ACGT 3 cut(s) 295, 387, 594
TaqI TCGA 2 cut(s) 208, 295
TasI AATT 6 cut(s) 376, 477, 661, 727, 832, 842
TauI GCSGC 1 cut(s) 600
TfiI GAWTC 3 cut(s) 58, 581, 767
Tru1I TTAA 3 cut(s) 546, 831, 845
Tru9I TTAA 3 cut(s) 546, 831, 845
TscAI CASTG 4 cut(s) 260, 326, 579, 795
TseFI GTSAC 2 cut(s) 574, 919
Tsp45I GTSAC 2 cut(s) 574, 919
TspDTI ATGAA 3 cut(s) 315, 707, 899
TspGWI ACGGA 6 cut(s) 186, 532, 570, 574, 609, 799
TspMI CCCGGG 1 cut(s) 53
TspRI CASTG 4 cut(s) 260, 326, 579, 795
Van91I CCANNNNNTGG 1 cut(s) 794
VneI GTGCAC 1 cut(s) 587
VpaK11BI GGWCC 4 cut(s) 17, 307, 617, 654
VspI ATTAAT 1 cut(s) 831
XapI RAATTY 2 cut(s) 376, 661
XceI RCATGY 2 cut(s) 911, 943
XcmI CCANNNNNNNNNTGG 1 cut(s) 232
XmaI CCCGGG 1 cut(s) 53
XspI CTAG 2 cut(s) 341, 396
ZraI GACGTC 1 cut(s) 385
Zsp2I ATGCAT 2 cut(s) 879, 1005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.