pycom01g00140

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000513
Physical Location & Seq
Forward (+)
326833 .. 328402
1570 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00140.6

Sequence Viewer

Length: 1173 bp
ATGAAATACCAAATAGGCTTGAACCATACTAATTGCTATCTCTATCTGCACAGACATAAGCACATGGAGCAAAAGAAAATCTATCGTACTGCTATATATAGAAATACACATTTAATCTCAAGTAAGAAAAGTGATATTGAAAGAAAACCTAATTACTGTCATACAAACCTAAATCCCAAATTATACACAAATTATGCCTTTATAGTTTTACTGCTTCCTATAACCTACCTAAAAATCATATACTTAATAAATGCGGCAAGTAGAAGATTGGATATTCGATCAGCGAATATGGTTATCAATTATGATATCCCCAAAAACTGTGACAGCTCGTTCCGAAAATTCTTAAACGTACGCGTGAAAAGACGATTTTTGGTGTGGCTGGTTGTGGACCACACACAATTCCACACACTCACCCTCACCATCACCATTTCTGCCCTGCACCCCCTGTCCCGAGCCCTCTCTCTCCCATTTTCCCCCTCCATGTGTACAGACACATTACAAAACCCTTGTAATCTTCACAGATCGAAGAAACAAAGTACATATCCATACCTTCGTTTTCATGTCGAACTCGGGATACCCGATTTTTGGCACTGTTCATACACACGTAAATTATTATGTTTTTGGGAATTTCAAGCTTGTAGGAAGCTTGGTGAGGTCCTTAGGAGGCTCGGGGTGGTTGGGTTGAAGCTTTTGGACGTCGAAATTGCGAGTTTCGAGGTTGGCTGGAGTTGGGGGTATTTTCCCGGCGAGATTTCATGGATTTTAGTACTTGAAAGTGGTATGATTTTGTTCCTCTCATTTTCCCGATTTTCCGGCGCAGGGGCCTCGCCGGAGAAGACGACGGAATATTCCGTTAGTTTGGACGGAATATTCCTAACGCTGTTGCCAGAATCCGTTAAAGTTAACAGAATATTCCTGACGCCGTCAGCGTGCAAGGCACGTGCCTGCGCGTGGCCGACGCGTGTGGCCATGCCTTGGCCGGCGCGTAGGGGTATCACGTTGGTGTATTCATACACCCCATTTGAGCATTGTATGAGAAGTTATTTCTTAAGGTTGGTTATGTGCTTTAAAATTAACATTTTTATAGTTATTTCGCATATAGGTGATACTTACCCCGAGGACGAGCGTGGTCACTCGAGGCTAGGGGGTTACGACCCTTCCACATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

391

Amino Acids

45.43

Weight (kDa)

9.56

Isoelectric Point (pI)

43.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 699
AccB7I CCANNNNNTGG 1 cut(s) 975
AccII CGCG 4 cut(s) 354, 950, 961, 985
AciI CCGC 1 cut(s) 254
AcoI YGGCCR 3 cut(s) 953, 966, 977
AcsI RAATTY 2 cut(s) 338, 626
AcvI CACGTG 1 cut(s) 941
AcyI GRCGYC 2 cut(s) 696, 920
AfaI GTAC 5 cut(s) 88, 351, 487, 538, 768
AfiI CCNNNNNNNGG 4 cut(s) 585, 819, 951, 975
AflII CTTAAG 1 cut(s) 1048
AflIII ACRYGT 3 cut(s) 352, 602, 959
AgsI TTSAA 5 cut(s) 22, 140, 632, 685, 773
AleI CACNNNNGTG 1 cut(s) 1001
AluBI AGCT 4 cut(s) 327, 635, 646, 688
AluI AGCT 4 cut(s) 327, 635, 646, 688
Ama87I CYCGRG 5 cut(s) 450, 569, 668, 1115, 1135
AoxI GGCC 4 cut(s) 822, 953, 966, 977
ApoI RAATTY 2 cut(s) 338, 626
AspLEI GCGC 3 cut(s) 818, 950, 985
AspS9I GGNCC 3 cut(s) 388, 655, 822
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 5 cut(s) 403, 409, 415, 662, 1115
AvaI CYCGRG 5 cut(s) 450, 569, 668, 1115, 1135
AvaII GGWCC 2 cut(s) 388, 655
AxyI CCTNAGG 1 cut(s) 659
BalI TGGCCA 1 cut(s) 968
BanII GRGCYC 1 cut(s) 457
BbrPI CACGTG 1 cut(s) 941
BbsI GAAGAC 1 cut(s) 842
BccI CCATC 1 cut(s) 428
BceAI ACGGC 1 cut(s) 907
BcgI CGANNNNNNTGC 2 cut(s) 807, 841
BciVI GTATCC 1 cut(s) 567
BcnI CCSGG 1 cut(s) 744
BfaI CTAG 1 cut(s) 1142
BfrI CTTAAG 1 cut(s) 1048
BfuI GTATCC 1 cut(s) 567
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
BmcAI AGTACT 1 cut(s) 768
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 2 cut(s) 388, 655
BmeT110I CYCGRG 5 cut(s) 450, 569, 668, 1115, 1135
BmgT120I GGNCC 3 cut(s) 388, 655, 822
BmiI GGNNCC 1 cut(s) 823
BmrFI CCNGG 1 cut(s) 744
BpiI GAAGAC 1 cut(s) 842
BpmI CTGGAG 1 cut(s) 745
BpuEI CTTGAG 1 cut(s) 103
BpuMI CCSGG 1 cut(s) 744
BsaAI YACGTR 2 cut(s) 605, 941
BsaHI GRCGYC 2 cut(s) 696, 920
BsaJI CCNNGG 2 cut(s) 974, 1116
Bsc4I CCNNNNNNNGG 4 cut(s) 585, 819, 951, 975
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 1 cut(s) 1168
Bse21I CCTNAGG 1 cut(s) 659
BseDI CCNNGG 2 cut(s) 974, 1116
BseLI CCNNNNNNNGG 4 cut(s) 585, 819, 951, 975
BseNI ACTGG 1 cut(s) 1168
BsgI GTGCAG 2 cut(s) 32, 422
Bsh1236I CGCG 4 cut(s) 354, 950, 961, 985
BshFI GGCC 4 cut(s) 824, 955, 968, 979
BsiHKCI CYCGRG 5 cut(s) 450, 569, 668, 1115, 1135
BsiSI CCGG 4 cut(s) 744, 813, 830, 980
BsiWI CGTACG 1 cut(s) 349
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 4 cut(s) 585, 819, 951, 975
BsmFI GGGAC 1 cut(s) 433
BsnI GGCC 4 cut(s) 824, 955, 968, 979
BsoBI CYCGRG 5 cut(s) 450, 569, 668, 1115, 1135
Bsp1286I GDGCHC 1 cut(s) 457
Bsp1407I TGTACA 1 cut(s) 485
Bsp143I GATC 2 cut(s) 278, 521
BspACI CCGC 1 cut(s) 254
BspANI GGCC 4 cut(s) 824, 955, 968, 979
BspFNI CGCG 4 cut(s) 354, 950, 961, 985
BspLI GGNNCC 1 cut(s) 823
BspTI CTTAAG 1 cut(s) 1048
BsrFI RCCGGY 1 cut(s) 979
BsrGI TGTACA 1 cut(s) 485
BsrI ACTGG 1 cut(s) 1168
BssAI RCCGGY 1 cut(s) 979
BssECI CCNNGG 2 cut(s) 974, 1116
BssMI GATC 2 cut(s) 278, 521
BssNI GRCGYC 2 cut(s) 696, 920
BssT1I CCWWGG 1 cut(s) 974
Bst4CI ACNGT 3 cut(s) 158, 320, 593
BstACI GRCGYC 2 cut(s) 696, 920
BstAFI CTTAAG 1 cut(s) 1048
BstAUI TGTACA 1 cut(s) 485
BstBAI YACGTR 2 cut(s) 605, 941
BstC8I GCNNGC 3 cut(s) 931, 946, 981
BstDEI CTNAG 1 cut(s) 659
BstFNI CGCG 4 cut(s) 354, 950, 961, 985
BstHHI GCGC 3 cut(s) 818, 950, 985
BstKTI GATC 2 cut(s) 281, 524
BstMBI GATC 2 cut(s) 278, 521
BstMWI GCNNNNNNNGC 2 cut(s) 67, 935
BstSCI CCNGG 1 cut(s) 742
BstUI CGCG 4 cut(s) 354, 950, 961, 985
BstV2I GAAGAC 1 cut(s) 842
Bsu36I CCTNAGG 1 cut(s) 659
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 4 cut(s) 824, 955, 968, 979
BtsIMutI CAGTG 1 cut(s) 589
Cac8I GCNNGC 3 cut(s) 931, 946, 981
CfoI GCGC 3 cut(s) 818, 950, 985
Cfr10I RCCGGY 1 cut(s) 979
Cfr13I GGNCC 3 cut(s) 388, 655, 822
CseI GACGC 2 cut(s) 928, 967
Csp6I GTAC 5 cut(s) 87, 350, 486, 537, 767
CviAII CATG 5 cut(s) 64, 481, 560, 756, 970
CviQI GTAC 5 cut(s) 87, 350, 486, 537, 767
DdeI CTNAG 1 cut(s) 659
DpnI GATC 2 cut(s) 280, 523
DpnII GATC 2 cut(s) 278, 521
DraI TTTAAA 1 cut(s) 1069
EaeI YGGCCR 3 cut(s) 953, 966, 977
Eco130I CCWWGG 1 cut(s) 974
Eco24I GRGCYC 1 cut(s) 457
Eco32I GATATC 1 cut(s) 307
Eco47I GGWCC 2 cut(s) 388, 655
Eco72I CACGTG 1 cut(s) 941
Eco81I CCTNAGG 1 cut(s) 659
Eco88I CYCGRG 5 cut(s) 450, 569, 668, 1115, 1135
EcoO109I RGGNCCY 2 cut(s) 655, 822
EcoRV GATATC 1 cut(s) 307
EcoT14I CCWWGG 1 cut(s) 974
EcoT38I GRGCYC 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 974
FaeI CATG 5 cut(s) 67, 484, 563, 759, 973
FaqI GGGAC 1 cut(s) 433
FatI CATG 5 cut(s) 63, 480, 559, 755, 969
Fnu4HI GCNGC 1 cut(s) 255
FriOI GRGCYC 1 cut(s) 457
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 1 cut(s) 1142
GlaI GCGC 3 cut(s) 817, 949, 984
GluI GCNGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 745
HaeIII GGCC 4 cut(s) 824, 955, 968, 979
HapII CCGG 4 cut(s) 744, 813, 830, 980
HgaI GACGC 2 cut(s) 928, 967
HhaI GCGC 3 cut(s) 818, 950, 985
Hin1I GRCGYC 2 cut(s) 696, 920
Hin1II CATG 5 cut(s) 67, 484, 563, 759, 973
Hin6I GCGC 3 cut(s) 816, 948, 983
HinP1I GCGC 3 cut(s) 816, 948, 983
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HindIII AAGCTT 3 cut(s) 633, 644, 686
HinfI GANTC 1 cut(s) 890
HpaI GTTAAC 1 cut(s) 904
HpaII CCGG 4 cut(s) 744, 813, 830, 980
HphI GGTGA 5 cut(s) 403, 409, 415, 662, 1115
Hpy166II GTNNAC 3 cut(s) 388, 486, 904
Hpy188I TCNGA 1 cut(s) 335
Hpy188III TCNNGA 4 cut(s) 450, 571, 804, 916
Hpy8I GTNNAC 3 cut(s) 388, 486, 904
Hpy99I CGWCG 3 cut(s) 701, 844, 961
HpyAV CCTTC 2 cut(s) 560, 1167
HpyCH4III ACNGT 3 cut(s) 158, 320, 593
HpyCH4IV ACGT 5 cut(s) 348, 604, 696, 940, 998
HpyCH4V TGCA 3 cut(s) 49, 439, 933
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 935
HpyF3I CTNAG 1 cut(s) 659
HpySE526I ACGT 5 cut(s) 348, 604, 696, 940, 998
Hsp92I GRCGYC 2 cut(s) 696, 920
Hsp92II CATG 5 cut(s) 67, 484, 563, 759, 973
HspAI GCGC 3 cut(s) 816, 948, 983
KroI GCCGGC 1 cut(s) 979
KroNI GCCGGC 1 cut(s) 981
KspAI GTTAAC 1 cut(s) 904
Kzo9I GATC 2 cut(s) 278, 521
LmnI GCTCC 1 cut(s) 67
MaeI CTAG 1 cut(s) 1142
MaeII ACGT 5 cut(s) 348, 604, 696, 940, 998
MaeIII GTNAC 3 cut(s) 320, 1130, 1148
MalI GATC 2 cut(s) 280, 523
MboI GATC 2 cut(s) 278, 521
MboII GAAGA 4 cut(s) 276, 506, 538, 847
MhlI GDGCHC 1 cut(s) 457
MlsI TGGCCA 1 cut(s) 968
MluI ACGCGT 2 cut(s) 352, 959
MluNI TGGCCA 1 cut(s) 968
Mox20I TGGCCA 1 cut(s) 968
MroNI GCCGGC 1 cut(s) 979
MscI TGGCCA 1 cut(s) 968
MseI TTAA 8 cut(s) 113, 245, 344, 897, 903, 1049, 1068, 1074
MslI CAYNNNNRTG 3 cut(s) 1001, 1101, 1168
Msp20I TGGCCA 1 cut(s) 968
MspCI CTTAAG 1 cut(s) 1048
MspI CCGG 4 cut(s) 744, 813, 830, 980
MspR9I CCNGG 1 cut(s) 744
MvnI CGCG 4 cut(s) 354, 950, 961, 985
MwoI GCNNNNNNNGC 2 cut(s) 67, 935
NaeI GCCGGC 1 cut(s) 981
NciI CCSGG 1 cut(s) 744
NdeII GATC 2 cut(s) 278, 521
NgoMIV GCCGGC 1 cut(s) 979
NlaIII CATG 5 cut(s) 67, 484, 563, 759, 973
NlaIV GGNNCC 1 cut(s) 823
NmuCI GTSAC 2 cut(s) 320, 1130
OliI CACNNNNGTG 1 cut(s) 1001
PaeR7I CTCGAG 1 cut(s) 1135
PcsI WCGNNNNNNNCGW 2 cut(s) 576, 926
PdiI GCCGGC 1 cut(s) 981
PfeI GAWTC 1 cut(s) 890
Pfl23II CGTACG 1 cut(s) 349
PflFI GACNNNGTC 1 cut(s) 922
PflMI CCANNNNNTGG 1 cut(s) 975
PkrI GCNGC 1 cut(s) 256
PmaCI CACGTG 1 cut(s) 941
PmlI CACGTG 1 cut(s) 941
Ppu21I YACGTR 2 cut(s) 605, 941
PpuMI RGGWCCY 1 cut(s) 655
Psp5II RGGWCCY 1 cut(s) 655
PspCI CACGTG 1 cut(s) 941
PspLI CGTACG 1 cut(s) 349
PspN4I GGNNCC 1 cut(s) 823
PspPI GGNCC 3 cut(s) 388, 655, 822
PspPPI RGGWCCY 1 cut(s) 655
PspXI VCTCGAGB 1 cut(s) 1135
PsrI GAACNNNNNNTAC 2 cut(s) 558, 590
PsyI GACNNNGTC 1 cut(s) 922
RsaI GTAC 5 cut(s) 88, 351, 487, 538, 768
RsaNI GTAC 5 cut(s) 87, 350, 486, 537, 767
RseI CAYNNNNRTG 3 cut(s) 1001, 1101, 1168
SaqAI TTAA 8 cut(s) 113, 245, 344, 897, 903, 1049, 1068, 1074
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 2 cut(s) 278, 521
Sau96I GGNCC 3 cut(s) 388, 655, 822
ScaI AGTACT 1 cut(s) 768
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 1 cut(s) 457
Sfr274I CTCGAG 1 cut(s) 1135
SinI GGWCC 2 cut(s) 388, 655
SlaI CTCGAG 1 cut(s) 1135
SmiMI CAYNNNNRTG 3 cut(s) 1001, 1101, 1168
SmlI CTYRAG 3 cut(s) 118, 1048, 1135
SmoI CTYRAG 3 cut(s) 118, 1048, 1135
SsiI CCGC 1 cut(s) 254
SspI AATATT 3 cut(s) 848, 870, 912
SspMI CTAG 1 cut(s) 1142
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 1 cut(s) 974
TaaI ACNGT 3 cut(s) 158, 320, 593
TaiI ACGT 5 cut(s) 351, 607, 699, 943, 1001
TaqI TCGA 6 cut(s) 277, 524, 564, 699, 714, 1136
TatI WGTACW 3 cut(s) 485, 536, 766
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 1 cut(s) 890
Tru1I TTAA 8 cut(s) 113, 245, 344, 897, 903, 1049, 1068, 1074
Tru9I TTAA 8 cut(s) 113, 245, 344, 897, 903, 1049, 1068, 1074
TscAI CASTG 1 cut(s) 596
TseFI GTSAC 2 cut(s) 320, 1130
Tsp45I GTSAC 2 cut(s) 320, 1130
TspDTI ATGAA 5 cut(s) 17, 548, 585, 744, 999
TspGWI ACGGA 4 cut(s) 841, 857, 879, 883
TspRI CASTG 1 cut(s) 596
Tth111I GACNNNGTC 1 cut(s) 922
Van91I CCANNNNNTGG 1 cut(s) 975
Vha464I CTTAAG 1 cut(s) 1048
VpaK11BI GGWCC 2 cut(s) 388, 655
XapI RAATTY 2 cut(s) 338, 626
XhoI CTCGAG 1 cut(s) 1135
XspI CTAG 1 cut(s) 1142
ZraI GACGTC 1 cut(s) 697
ZrmI AGTACT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.