pycom13g28360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000513
Physical Location & Seq
Forward (+)
24236456 .. 24238044
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g28360.1

Sequence Viewer

Length: 1176 bp
ATGACCCTTCTACATACCAGTAAGTGGGTCCTTTCCTATGCATGTTACCTACAAATAATTGATGCTAACTACGAATGGCTTGATCCCTGTTTAGGGTATGTAGGCAGTCTAACGAGACACAATAGCCTTGTTTGGGGAATTGAGCAATGTGAGGGACATTGGTGGAAAATAAATCAATGTTTGGACTTCTTCCAGATAATTATTCCCGAAATAAATAGTTCGGGAGTGGGGCATTATAGATTATGCATATTTCACCATGTTATATATATGTATATATTGGTAAATGCTATGTATACTACCGTAAGTAATTACTGTGAATGCTTTGAAATGCGAAGAGGAACACAGGACGCACAAGTAAGTTCATGTGAGTTTATGGTGTTACTTGAAACGATCGAGTTATGGTATGACTATATTTATGCTTTAGGAAACTCCGACCTTGTCGTACAGGTTGCAAGGATAGGCAACTCTGACATTGTCCTAGAGCTCATAAGCCTGCACCCCGGTGTTAGGGCTCCCGCCCGTGGCCAGGGCACAGTCCTTCACGTGATGTTCACCTCTGGCACCTTATACTCACCTTGGATCCAAGGTAGGTGCACAGCCTTGTCGTATAGACCACTACATGTGGTTCCGACCGTAGCACTTGAGCGTACTTATTTACACGTAGTCCTGTCGTACAGACCACTAGAGGTGGTTCTGATAGGAGCATATTTATGCTACTTAGCTATGTTGTTTCCTTGCATTTGGATAGTTAATTACTACTTATTAAAGTCTGTTAACAAGAAAGTACATTTTGGAGCTATTTGGAGCGGTTTTGGGCATGGATTGGATAGCTTAACCATAGAGCAAAGTGGATGGACGAAATTGAAGTCAAGAGAGGCTAGGAAAGGCTACAAATCTGGAGAAAGAAGTTCTAATGCAAAGAAGATAAGGAAAGAGCAAGAAATAAGGAACCTTATCCAACCTTATCTTATCCTACCTTATCCAACCTTATCCTTTCCTAAATCTAACATTATCCTATCTTATCCTATCCAAATCAGGTTGTGCCTTAAGTCTAGCTATTTAGAAGGGATTAGATATACATTTAAACACATAAACTCATATTCTAGAAGCCTAAGCCTTTATCCTTCTCCTACAAAGGTGCCGCACCCCTTAGTCTTTGTTCATCTAGAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

392

Amino Acids

45.03

Weight (kDa)

8.78

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 560, 1138
AccB7I CCANNNNNTGG 1 cut(s) 24
AccBSI CCGCTC 1 cut(s) 807
AccI GTMKAC 1 cut(s) 293
AciI CCGC 3 cut(s) 516, 807, 1142
AclWI GGATC 3 cut(s) 77, 574, 587
AcoI YGGCCR 1 cut(s) 523
AcvI CACGTG 1 cut(s) 544
AfaI GTAC 4 cut(s) 444, 649, 674, 786
AfiI CCNNNNNNNGG 7 cut(s) 24, 92, 93, 133, 507, 521, 526
AflII CTTAAG 1 cut(s) 1046
AflIII ACRYGT 2 cut(s) 619, 658
AgsI TTSAA 3 cut(s) 326, 386, 865
AjnI CCWGG 1 cut(s) 525
AleI CACNNNNGTG 1 cut(s) 501
AluBI AGCT 5 cut(s) 484, 722, 797, 831, 1056
AluI AGCT 5 cut(s) 484, 722, 797, 831, 1056
Alw21I GWGCWC 2 cut(s) 486, 596
Alw26I GTCTC 1 cut(s) 109
Alw44I GTGCAC 1 cut(s) 592
AlwI GGATC 3 cut(s) 77, 574, 587
AoxI GGCC 1 cut(s) 523
ApaLI GTGCAC 1 cut(s) 592
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
AspS9I GGNCC 1 cut(s) 28
AsuC2I CCSGG 1 cut(s) 501
AsuHPI GGTGA 3 cut(s) 245, 544, 564
AvaII GGWCC 1 cut(s) 28
BaeGI GKGCMC 2 cut(s) 533, 596
BalI TGGCCA 1 cut(s) 525
BamHI GGATCC 1 cut(s) 579
BanI GGYRCC 2 cut(s) 560, 1138
BanII GRGCYC 2 cut(s) 486, 514
BbrPI CACGTG 1 cut(s) 544
Bbv12I GWGCWC 2 cut(s) 486, 596
BccI CCATC 1 cut(s) 846
BciT130I CCWGG 1 cut(s) 527
BcnI CCSGG 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 109
BfaI CTAG 6 cut(s) 479, 683, 879, 1053, 1104, 1166
BfrI CTTAAG 1 cut(s) 1046
BisI GCNGC 1 cut(s) 1142
BlsI GCNGC 1 cut(s) 1143
Bme1390I CCNGG 2 cut(s) 501, 527
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 7 cut(s) 29, 513, 562, 581, 627, 950, 1140
BmrFI CCNGG 2 cut(s) 501, 527
BmsI GCATC 1 cut(s) 52
BpmI CTGGAG 1 cut(s) 918
Bpu10I CCTNAGC 1 cut(s) 1112
BpuEI CTTGAG 1 cut(s) 662
BpuMI CCSGG 1 cut(s) 501
BsaAI YACGTR 2 cut(s) 544, 661
BsaJI CCNNGG 5 cut(s) 499, 520, 526, 575, 583
Bsc4I CCNNNNNNNGG 7 cut(s) 24, 92, 93, 133, 507, 521, 526
Bse1I ACTGG 1 cut(s) 18
Bse3DI GCAATG 1 cut(s) 152
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 5 cut(s) 499, 520, 526, 575, 583
BseGI GGATG 1 cut(s) 857
BseLI CCNNNNNNNGG 7 cut(s) 24, 92, 93, 133, 507, 521, 526
BseMI GCAATG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 18
BseSI GKGCMC 2 cut(s) 533, 596
BsgI GTGCAG 1 cut(s) 479
Bsh1285I CGRYCG 2 cut(s) 393, 633
BshFI GGCC 1 cut(s) 525
BshNI GGYRCC 2 cut(s) 560, 1138
BsiEI CGRYCG 2 cut(s) 393, 633
BsiHKAI GWGCWC 2 cut(s) 486, 596
BsiSI CCGG 1 cut(s) 501
BslFI GGGAC 1 cut(s) 168
BslI CCNNNNNNNGG 7 cut(s) 24, 92, 93, 133, 507, 521, 526
BsmAI GTCTC 1 cut(s) 109
BsmFI GGGAC 1 cut(s) 168
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 1 cut(s) 525
Bsp1286I GDGCHC 4 cut(s) 486, 514, 533, 596
Bsp143I GATC 3 cut(s) 82, 390, 579
BspACI CCGC 3 cut(s) 516, 807, 1142
BspANI GGCC 1 cut(s) 525
BspLI GGNNCC 7 cut(s) 29, 513, 562, 581, 627, 950, 1140
BspPI GGATC 3 cut(s) 77, 574, 587
BspT107I GGYRCC 2 cut(s) 560, 1138
BspTI CTTAAG 1 cut(s) 1046
BsrBI CCGCTC 1 cut(s) 807
BsrDI GCAATG 1 cut(s) 152
BsrI ACTGG 1 cut(s) 18
BssECI CCNNGG 5 cut(s) 499, 520, 526, 575, 583
BssMI GATC 3 cut(s) 82, 390, 579
BssNAI GTATAC 1 cut(s) 294
BssT1I CCWWGG 2 cut(s) 575, 583
Bst1107I GTATAC 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 527
Bst4CI ACNGT 4 cut(s) 301, 314, 535, 634
Bst6I CTCTTC 1 cut(s) 328
BstAFI CTTAAG 1 cut(s) 1046
BstBAI YACGTR 2 cut(s) 544, 661
BstC8I GCNNGC 1 cut(s) 494
BstDEI CTNAG 3 cut(s) 718, 1112, 1150
BstDSI CCRYGG 1 cut(s) 520
BstF5I GGATG 1 cut(s) 857
BstKTI GATC 3 cut(s) 85, 393, 582
BstMAI GTCTC 1 cut(s) 109
BstMBI GATC 3 cut(s) 82, 390, 579
BstMCI CGRYCG 2 cut(s) 393, 633
BstNI CCWGG 1 cut(s) 527
BstNSI RCATGY 2 cut(s) 45, 623
BstSCI CCNGG 2 cut(s) 499, 525
BstSLI GKGCMC 2 cut(s) 533, 596
BstX2I RGATCY 1 cut(s) 579
BstYI RGATCY 1 cut(s) 579
BstZ17I GTATAC 1 cut(s) 294
BsuRI GGCC 1 cut(s) 525
BtgI CCRYGG 1 cut(s) 520
BtsCI GGATG 1 cut(s) 857
Cac8I GCNNGC 1 cut(s) 494
Cfr13I GGNCC 1 cut(s) 28
CseI GACGC 1 cut(s) 356
Csp6I GTAC 4 cut(s) 443, 648, 673, 785
CviAII CATG 5 cut(s) 42, 257, 363, 620, 818
CviQI GTAC 4 cut(s) 443, 648, 673, 785
DdeI CTNAG 3 cut(s) 718, 1112, 1150
DpnI GATC 3 cut(s) 84, 392, 581
DpnII GATC 3 cut(s) 82, 390, 579
DraI TTTAAA 1 cut(s) 1084
EaeI YGGCCR 1 cut(s) 523
Eam1104I CTCTTC 1 cut(s) 328
EarI CTCTTC 1 cut(s) 328
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 2 cut(s) 575, 583
Eco24I GRGCYC 2 cut(s) 486, 514
Eco47I GGWCC 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 484
Eco72I CACGTG 1 cut(s) 544
EcoICRI GAGCTC 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 525
EcoT14I CCWWGG 2 cut(s) 575, 583
EcoT22I ATGCAT 2 cut(s) 43, 248
EcoT38I GRGCYC 2 cut(s) 486, 514
ErhI CCWWGG 2 cut(s) 575, 583
FaeI CATG 5 cut(s) 45, 260, 366, 623, 821
FaqI GGGAC 1 cut(s) 168
FatI CATG 5 cut(s) 41, 256, 362, 619, 817
FauI CCCGC 1 cut(s) 523
FblI GTMKAC 1 cut(s) 293
Fnu4HI GCNGC 1 cut(s) 1142
FokI GGATG 1 cut(s) 864
FriOI GRGCYC 2 cut(s) 486, 514
Fsp4HI GCNGC 1 cut(s) 1142
FspBI CTAG 6 cut(s) 479, 683, 879, 1053, 1104, 1166
GluI GCNGC 1 cut(s) 1142
GsuI CTGGAG 1 cut(s) 918
HaeIII GGCC 1 cut(s) 525
HapII CCGG 1 cut(s) 501
HgaI GACGC 1 cut(s) 356
Hin1II CATG 5 cut(s) 45, 260, 366, 623, 821
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HpaI GTTAAC 1 cut(s) 775
HpaII CCGG 1 cut(s) 501
HphI GGTGA 3 cut(s) 245, 544, 564
Hpy166II GTNNAC 4 cut(s) 294, 552, 594, 775
Hpy188I TCNGA 4 cut(s) 433, 469, 630, 696
Hpy188III TCNNGA 7 cut(s) 193, 206, 222, 870, 897, 1104, 1166
Hpy8I GTNNAC 4 cut(s) 294, 552, 594, 775
HpyAV CCTTC 4 cut(s) 17, 548, 1058, 1134
HpyCH4III ACNGT 4 cut(s) 301, 314, 535, 634
HpyCH4IV ACGT 2 cut(s) 543, 660
HpyCH4V TGCA 7 cut(s) 41, 246, 452, 496, 594, 738, 917
HpyF3I CTNAG 3 cut(s) 718, 1112, 1150
HpySE526I ACGT 2 cut(s) 543, 660
Hsp92II CATG 5 cut(s) 45, 260, 366, 623, 821
KspAI GTTAAC 1 cut(s) 775
Kzo9I GATC 3 cut(s) 82, 390, 579
LmnI GCTCC 4 cut(s) 517, 701, 794, 804
LweI GCATC 1 cut(s) 52
MaeI CTAG 6 cut(s) 479, 683, 879, 1053, 1104, 1166
MaeII ACGT 2 cut(s) 543, 660
MaeIII GTNAC 2 cut(s) 44, 378
MalI GATC 3 cut(s) 84, 392, 581
MbiI CCGCTC 1 cut(s) 807
MboI GATC 3 cut(s) 82, 390, 579
MboII GAAGA 3 cut(s) 181, 345, 934
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 4 cut(s) 486, 514, 533, 596
MlsI TGGCCA 1 cut(s) 525
MluCI AATT 6 cut(s) 57, 138, 198, 307, 751, 860
MluNI TGGCCA 1 cut(s) 525
MmeI TCCRAC 4 cut(s) 456, 653, 982, 1007
MnlI CCTC 5 cut(s) 145, 329, 565, 679, 868
Mox20I TGGCCA 1 cut(s) 525
Mph1103I ATGCAT 2 cut(s) 43, 248
MscI TGGCCA 1 cut(s) 525
MseI TTAA 6 cut(s) 750, 764, 774, 833, 1047, 1083
MslI CAYNNNNRTG 2 cut(s) 501, 709
Msp20I TGGCCA 1 cut(s) 525
MspCI CTTAAG 1 cut(s) 1046
MspI CCGG 1 cut(s) 501
MspR9I CCNGG 2 cut(s) 501, 527
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 1 cut(s) 527
NciI CCSGG 1 cut(s) 501
NdeII GATC 3 cut(s) 82, 390, 579
NlaIII CATG 5 cut(s) 45, 260, 366, 623, 821
NlaIV GGNNCC 7 cut(s) 29, 513, 562, 581, 627, 950, 1140
NsiI ATGCAT 2 cut(s) 43, 248
NspI RCATGY 2 cut(s) 45, 623
OliI CACNNNNGTG 1 cut(s) 501
PciI ACATGT 1 cut(s) 619
PctI GAATGC 1 cut(s) 323
PflFI GACNNNGTC 2 cut(s) 437, 473
PflMI CCANNNNNTGG 1 cut(s) 24
PkrI GCNGC 1 cut(s) 1143
Ple19I CGATCG 1 cut(s) 393
PmaCI CACGTG 1 cut(s) 544
PmlI CACGTG 1 cut(s) 544
Ppu21I YACGTR 2 cut(s) 544, 661
PpuMI RGGWCCY 1 cut(s) 28
PscI ACATGT 1 cut(s) 619
Psp124BI GAGCTC 1 cut(s) 486
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 525
PspCI CACGTG 1 cut(s) 544
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 7 cut(s) 29, 513, 562, 581, 627, 950, 1140
PspPI GGNCC 1 cut(s) 28
PspPPI RGGWCCY 1 cut(s) 28
PsuI RGATCY 1 cut(s) 579
PsyI GACNNNGTC 2 cut(s) 437, 473
PvuI CGATCG 1 cut(s) 393
RsaI GTAC 4 cut(s) 444, 649, 674, 786
RsaNI GTAC 4 cut(s) 443, 648, 673, 785
RseI CAYNNNNRTG 2 cut(s) 501, 709
SacI GAGCTC 1 cut(s) 486
SaqAI TTAA 6 cut(s) 750, 764, 774, 833, 1047, 1083
SatI GCNGC 1 cut(s) 1142
Sau3AI GATC 3 cut(s) 82, 390, 579
Sau96I GGNCC 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 501, 527
SduI GDGCHC 4 cut(s) 486, 514, 533, 596
SfaNI GCATC 1 cut(s) 52
SinI GGWCC 1 cut(s) 28
SmiMI CAYNNNNRTG 2 cut(s) 501, 709
SmlI CTYRAG 2 cut(s) 641, 1046
SmoI CTYRAG 2 cut(s) 641, 1046
Sse9I AATT 6 cut(s) 57, 138, 198, 307, 751, 860
SsiI CCGC 3 cut(s) 516, 807, 1142
SspMI CTAG 6 cut(s) 479, 683, 879, 1053, 1104, 1166
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 2 cut(s) 499, 525
StyI CCWWGG 2 cut(s) 575, 583
TaaI ACNGT 4 cut(s) 301, 314, 535, 634
TaiI ACGT 2 cut(s) 546, 663
TaqI TCGA 1 cut(s) 393
TasI AATT 6 cut(s) 57, 138, 198, 307, 751, 860
TatI WGTACW 1 cut(s) 784
TauI GCSGC 1 cut(s) 1144
Tru1I TTAA 6 cut(s) 750, 764, 774, 833, 1047, 1083
Tru9I TTAA 6 cut(s) 750, 764, 774, 833, 1047, 1083
TspDTI ATGAA 2 cut(s) 351, 1151
Tth111I GACNNNGTC 2 cut(s) 437, 473
Van91I CCANNNNNTGG 1 cut(s) 24
Vha464I CTTAAG 1 cut(s) 1046
VneI GTGCAC 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 28
XbaI TCTAGA 2 cut(s) 1103, 1165
XceI RCATGY 2 cut(s) 45, 623
XmiI GTMKAC 1 cut(s) 293
XspI CTAG 6 cut(s) 479, 683, 879, 1053, 1104, 1166
Zsp2I ATGCAT 2 cut(s) 43, 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.