pycom01g00970

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0000513
Physical Location & Seq
Forward (+)
939866 .. 941272
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g00970.5

Sequence Viewer

Length: 1026 bp
ATGCGACAGCCCGTTCCGGAAATTTTTAAAACGTACGCGGTTGTTTTGAGCCTGGTGTGGCTGGTTTTGCACCACACACTATCCCCCACACATTTCCTTCACCCTTTCCCTGTCCCTGCACCTGTCCCGTGCCATTTCCCTTCCCCATTTTTTATCCCATTTGTACGGACATACCCCAAAACCCTTGTAATCTTCACAGATCGAAGAATCAAAGTACATATTCATGTAAGGATACCTTCGTTTTCACGTCGAACTCGGGATACCCGATTTTTGTCACTGTTCATGCACACGTTAATTCTTGTTTTTTGTGAATTTCAAGCTCGTAGGAAGCTTGGTGAGGTCCTTAGGAGGCTCGGGGTGGTTCGTTTGAAGGTTTTGGACGTCGGATCGATCGTGAGTTTCAAGGTTGGCCGAAGTTGGTGGTTGGTATGGATTTGTTCCTCTCATTGTAAGCTTCATTTTGGTACCAATTTTCGACGGCACCGGAGCCTCGCGGAGAAGACGACGGAATATTCCGTCAGTTTGGACAGAATATTCCTAACGGCGTTAACGGAATCCATTAAAGTTAACGGAATATTCCTGACGGCGTTAACTGACGCCTTCAACATGCCAGGCACGTGTAGCCGTGCCTTGGCCAGCGCGTGGGGGCTAGATCAATCATTGGTGTATTCATACACCCCATTTGAGCATTGTATGAGAAGTTATTTCATAAGGTTGGTTATGTGTTTTAAAATTAACGTTTTTATAGTTGTTTCGCATATAGGTGAGACCTATCCCGAGGACGAGCGTGGTCACTCGAGGCAAGGGGGTTACGACCCTTCCACATATCAATGCCATGCTTATAGTTTGTCATTGATTTACGCATTACGAATGGCATATGTTTATATATATGTTTGGTGCTGCGGACGCACAGGTAAATGTCAGGTGAGTTATGATTTATGTTTACTGTTCCCGCCCAGAGTTGGGCACAGTCCTTCACGTGATGTTCACCTCCCGCACCACACGCTCATCTTGGATCCAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

342

Amino Acids

39.49

Weight (kDa)

9.59

Isoelectric Point (pI)

51.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 384
Acc65I GGTACC 1 cut(s) 464
AccB1I GGYRCC 2 cut(s) 464, 480
AccB7I CCANNNNNTGG 1 cut(s) 642
AccII CGCG 3 cut(s) 38, 494, 641
AccIII TCCGGA 1 cut(s) 16
AciI CCGC 5 cut(s) 38, 494, 903, 953, 995
AclI AACGTT 1 cut(s) 738
AclWI GGATC 3 cut(s) 394, 1010, 1023
AcoI YGGCCR 2 cut(s) 409, 633
AcsI RAATTY 2 cut(s) 21, 311
AcvI CACGTG 2 cut(s) 618, 980
AcyI GRCGYC 2 cut(s) 381, 597
AfaI GTAC 4 cut(s) 35, 165, 216, 466
AfiI CCNNNNNNNGG 4 cut(s) 631, 642, 962, 963
AflIII ACRYGT 2 cut(s) 288, 617
AgsI TTSAA 4 cut(s) 317, 370, 403, 604
AjiI CACGTC 1 cut(s) 248
AjnI CCWGG 2 cut(s) 51, 610
AluBI AGCT 3 cut(s) 320, 331, 454
AluI AGCT 3 cut(s) 320, 331, 454
Alw26I GTCTC 1 cut(s) 761
AlwI GGATC 3 cut(s) 394, 1010, 1023
Ama87I CYCGRG 4 cut(s) 255, 353, 776, 796
Aor13HI TCCGGA 1 cut(s) 16
AoxI GGCC 2 cut(s) 409, 633
ApeKI GCWGC 1 cut(s) 900
ApoI RAATTY 2 cut(s) 21, 311
Asp718I GGTACC 1 cut(s) 464
AspLEI GCGC 1 cut(s) 641
AspS9I GGNCC 1 cut(s) 340
AsuHPI GGTGA 5 cut(s) 92, 347, 776, 937, 980
AvaI CYCGRG 4 cut(s) 255, 353, 776, 796
AvaII GGWCC 1 cut(s) 340
AxyI CCTNAGG 1 cut(s) 344
BaeGI GKGCMC 1 cut(s) 969
BaeI ACNNNNGTAYC 2 cut(s) 224, 257
BalI TGGCCA 1 cut(s) 635
BamHI GGATCC 1 cut(s) 1015
BanI GGYRCC 2 cut(s) 464, 480
BbrPI CACGTG 2 cut(s) 618, 980
BbsI GAAGAC 1 cut(s) 506
BbvI GCAGC 1 cut(s) 887
BceAI ACGGC 4 cut(s) 494, 558, 600, 609
BciT130I CCWGG 2 cut(s) 53, 612
BciVI GTATCC 2 cut(s) 225, 253
BcoDI GTCTC 1 cut(s) 761
BfaI CTAG 1 cut(s) 650
BfuI GTATCC 2 cut(s) 225, 253
BisI GCNGC 1 cut(s) 901
BlsI GCNGC 1 cut(s) 902
Bme1390I CCNGG 2 cut(s) 53, 612
Bme18I GGWCC 1 cut(s) 340
BmeT110I CYCGRG 4 cut(s) 255, 353, 776, 796
BmgBI CACGTC 1 cut(s) 248
BmgT120I GGNCC 1 cut(s) 340
BmiI GGNNCC 4 cut(s) 466, 482, 488, 1017
BmrFI CCNGG 2 cut(s) 53, 612
BpiI GAAGAC 1 cut(s) 506
Bsa29I ATCGAT 1 cut(s) 389
BsaAI YACGTR 2 cut(s) 618, 980
BsaHI GRCGYC 2 cut(s) 381, 597
BsaI GGTCTC 1 cut(s) 761
BsaJI CCNNGG 2 cut(s) 630, 777
BsaWI WCCGGW 2 cut(s) 16, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 631, 642, 962, 963
Bse21I CCTNAGG 1 cut(s) 344
BseAI TCCGGA 1 cut(s) 16
BseBI CCWGG 2 cut(s) 53, 612
BseCI ATCGAT 1 cut(s) 389
BseDI CCNNGG 2 cut(s) 630, 777
BseLI CCNNNNNNNGG 4 cut(s) 631, 642, 962, 963
BseSI GKGCMC 1 cut(s) 969
BseXI GCAGC 1 cut(s) 887
BsgI GTGCAG 1 cut(s) 102
Bsh1236I CGCG 3 cut(s) 38, 494, 641
Bsh1285I CGRYCG 1 cut(s) 393
BshFI GGCC 2 cut(s) 411, 635
BshNI GGYRCC 2 cut(s) 464, 480
BshVI ATCGAT 1 cut(s) 389
BsiEI CGRYCG 1 cut(s) 393
BsiHKCI CYCGRG 4 cut(s) 255, 353, 776, 796
BsiSI CCGG 2 cut(s) 17, 484
BsiWI CGTACG 1 cut(s) 33
BslFI GGGAC 2 cut(s) 98, 110
BslI CCNNNNNNNGG 4 cut(s) 631, 642, 962, 963
BsmAI GTCTC 1 cut(s) 761
BsmFI GGGAC 2 cut(s) 98, 110
BsnI GGCC 2 cut(s) 411, 635
Bso31I GGTCTC 1 cut(s) 761
BsoBI CYCGRG 4 cut(s) 255, 353, 776, 796
Bsp1286I GDGCHC 1 cut(s) 969
Bsp13I TCCGGA 1 cut(s) 16
Bsp143I GATC 5 cut(s) 199, 386, 390, 652, 1015
BspACI CCGC 5 cut(s) 38, 494, 903, 953, 995
BspANI GGCC 2 cut(s) 411, 635
BspDI ATCGAT 1 cut(s) 389
BspEI TCCGGA 1 cut(s) 16
BspFNI CGCG 3 cut(s) 38, 494, 641
BspLI GGNNCC 4 cut(s) 466, 482, 488, 1017
BspPI GGATC 3 cut(s) 394, 1010, 1023
BspT107I GGYRCC 2 cut(s) 464, 480
BspTNI GGTCTC 1 cut(s) 761
BssECI CCNNGG 2 cut(s) 630, 777
BssMI GATC 5 cut(s) 199, 386, 390, 652, 1015
BssNI GRCGYC 2 cut(s) 381, 597
BssT1I CCWWGG 1 cut(s) 630
Bst2UI CCWGG 2 cut(s) 53, 612
Bst4CI ACNGT 3 cut(s) 279, 948, 971
BstACI GRCGYC 2 cut(s) 381, 597
BstBAI YACGTR 2 cut(s) 618, 980
BstC8I GCNNGC 1 cut(s) 637
BstDEI CTNAG 1 cut(s) 344
BstFNI CGCG 3 cut(s) 38, 494, 641
BstHHI GCGC 1 cut(s) 641
BstKTI GATC 5 cut(s) 202, 389, 393, 655, 1018
BstMAI GTCTC 1 cut(s) 761
BstMBI GATC 5 cut(s) 199, 386, 390, 652, 1015
BstMCI CGRYCG 1 cut(s) 393
BstMWI GCNNNNNNNGC 4 cut(s) 67, 621, 906, 1003
BstNI CCWGG 2 cut(s) 53, 612
BstNSI RCATGY 1 cut(s) 610
BstSCI CCNGG 2 cut(s) 51, 610
BstSLI GKGCMC 1 cut(s) 969
BstUI CGCG 3 cut(s) 38, 494, 641
BstV1I GCAGC 1 cut(s) 887
BstV2I GAAGAC 1 cut(s) 506
BstX2I RGATCY 1 cut(s) 1015
BstYI RGATCY 1 cut(s) 1015
Bsu15I ATCGAT 1 cut(s) 389
Bsu36I CCTNAGG 1 cut(s) 344
BsuI GTATCC 2 cut(s) 225, 253
BsuRI GGCC 2 cut(s) 411, 635
BsuTUI ATCGAT 1 cut(s) 389
BtrI CACGTC 1 cut(s) 248
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 637
CfoI GCGC 1 cut(s) 641
Cfr13I GGNCC 1 cut(s) 340
ClaI ATCGAT 1 cut(s) 389
CseI GACGC 2 cut(s) 605, 915
Csp6I GTAC 4 cut(s) 34, 164, 215, 465
CviAII CATG 4 cut(s) 224, 283, 607, 836
CviQI GTAC 4 cut(s) 34, 164, 215, 465
DdeI CTNAG 1 cut(s) 344
DpnI GATC 5 cut(s) 201, 388, 392, 654, 1017
DpnII GATC 5 cut(s) 199, 386, 390, 652, 1015
DraI TTTAAA 2 cut(s) 28, 730
EaeI YGGCCR 2 cut(s) 409, 633
Eco130I CCWWGG 1 cut(s) 630
Eco31I GGTCTC 1 cut(s) 761
Eco47I GGWCC 1 cut(s) 340
Eco72I CACGTG 2 cut(s) 618, 980
Eco81I CCTNAGG 1 cut(s) 344
Eco88I CYCGRG 4 cut(s) 255, 353, 776, 796
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 2 cut(s) 51, 610
EcoT14I CCWWGG 1 cut(s) 630
ErhI CCWWGG 1 cut(s) 630
FaeI CATG 4 cut(s) 227, 286, 610, 839
FalI AAGNNNNNCTT 2 cut(s) 220, 252
FaqI GGGAC 2 cut(s) 98, 110
FatI CATG 4 cut(s) 223, 282, 606, 835
FauI CCCGC 2 cut(s) 960, 1002
FauNDI CATATG 1 cut(s) 877
Fnu4HI GCNGC 1 cut(s) 901
Fsp4HI GCNGC 1 cut(s) 901
FspBI CTAG 1 cut(s) 650
GlaI GCGC 1 cut(s) 640
GluI GCNGC 1 cut(s) 901
HaeIII GGCC 2 cut(s) 411, 635
HapII CCGG 2 cut(s) 17, 484
HgaI GACGC 2 cut(s) 605, 915
HhaI GCGC 1 cut(s) 641
Hin1I GRCGYC 2 cut(s) 381, 597
Hin1II CATG 4 cut(s) 227, 286, 610, 839
Hin6I GCGC 1 cut(s) 639
HinP1I GCGC 1 cut(s) 639
HincII GTYRAC 3 cut(s) 549, 568, 591
HindII GTYRAC 3 cut(s) 549, 568, 591
HindIII AAGCTT 2 cut(s) 329, 452
HinfI GANTC 2 cut(s) 207, 554
HpaI GTTAAC 3 cut(s) 549, 568, 591
HpaII CCGG 2 cut(s) 17, 484
HphI GGTGA 5 cut(s) 92, 347, 776, 937, 980
Hpy166II GTNNAC 5 cut(s) 549, 568, 591, 944, 988
Hpy188I TCNGA 1 cut(s) 386
Hpy188III TCNNGA 5 cut(s) 17, 257, 394, 580, 776
Hpy8I GTNNAC 5 cut(s) 549, 568, 591, 944, 988
Hpy99I CGWCG 4 cut(s) 252, 386, 480, 508
HpyAV CCTTC 7 cut(s) 107, 150, 246, 364, 610, 828, 984
HpyCH4III ACNGT 3 cut(s) 279, 948, 971
HpyCH4IV ACGT 7 cut(s) 32, 247, 290, 381, 617, 738, 979
HpyCH4V TGCA 3 cut(s) 70, 119, 286
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 621, 906, 1003
HpyF3I CTNAG 1 cut(s) 344
HpySE526I ACGT 7 cut(s) 32, 247, 290, 381, 617, 738, 979
Hsp92I GRCGYC 2 cut(s) 381, 597
Hsp92II CATG 4 cut(s) 227, 286, 610, 839
HspAI GCGC 1 cut(s) 639
Kpn2I TCCGGA 1 cut(s) 16
KpnI GGTACC 1 cut(s) 468
KspAI GTTAAC 3 cut(s) 549, 568, 591
Kzo9I GATC 5 cut(s) 199, 386, 390, 652, 1015
LmnI GCTCC 1 cut(s) 486
Lsp1109I GCAGC 1 cut(s) 887
MaeI CTAG 1 cut(s) 650
MaeII ACGT 7 cut(s) 32, 247, 290, 381, 617, 738, 979
MaeIII GTNAC 3 cut(s) 273, 791, 809
MalI GATC 5 cut(s) 201, 388, 392, 654, 1017
MboI GATC 5 cut(s) 199, 386, 390, 652, 1015
MboII GAAGA 3 cut(s) 184, 216, 511
MflI RGATCY 1 cut(s) 1015
MhlI GDGCHC 1 cut(s) 969
MlsI TGGCCA 1 cut(s) 635
MluCI AATT 5 cut(s) 21, 294, 311, 469, 732
MluNI TGGCCA 1 cut(s) 635
MmeI TCCRAC 1 cut(s) 364
MnlI CCTC 7 cut(s) 331, 342, 451, 500, 772, 792, 1001
Mox20I TGGCCA 1 cut(s) 635
MroI TCCGGA 1 cut(s) 16
MscI TGGCCA 1 cut(s) 635
MseI TTAA 8 cut(s) 27, 293, 548, 561, 567, 590, 729, 735
MslI CAYNNNNRTG 3 cut(s) 222, 762, 829
Msp20I TGGCCA 1 cut(s) 635
MspI CCGG 2 cut(s) 17, 484
MspR9I CCNGG 2 cut(s) 53, 612
MvaI CCWGG 2 cut(s) 53, 612
MvnI CGCG 3 cut(s) 38, 494, 641
MwoI GCNNNNNNNGC 4 cut(s) 67, 621, 906, 1003
NdeI CATATG 1 cut(s) 877
NdeII GATC 5 cut(s) 199, 386, 390, 652, 1015
NlaIII CATG 4 cut(s) 227, 286, 610, 839
NlaIV GGNNCC 4 cut(s) 466, 482, 488, 1017
NmuCI GTSAC 2 cut(s) 273, 791
NspI RCATGY 1 cut(s) 610
PaeR7I CTCGAG 1 cut(s) 796
PcsI WCGNNNNNNNCGW 2 cut(s) 262, 390
PfeI GAWTC 2 cut(s) 207, 554
Pfl23II CGTACG 1 cut(s) 33
PflMI CCANNNNNTGG 1 cut(s) 642
PkrI GCNGC 1 cut(s) 902
Ple19I CGATCG 1 cut(s) 393
PmaCI CACGTG 2 cut(s) 618, 980
PmlI CACGTG 2 cut(s) 618, 980
Ppu21I YACGTR 2 cut(s) 618, 980
PpuMI RGGWCCY 1 cut(s) 340
Psp1406I AACGTT 1 cut(s) 738
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 2 cut(s) 51, 610
PspCI CACGTG 2 cut(s) 618, 980
PspGI CCWGG 2 cut(s) 51, 610
PspLI CGTACG 1 cut(s) 33
PspN4I GGNNCC 4 cut(s) 466, 482, 488, 1017
PspPI GGNCC 1 cut(s) 340
PspPPI RGGWCCY 1 cut(s) 340
PspXI VCTCGAGB 1 cut(s) 796
PsrI GAACNNNNNNTAC 2 cut(s) 244, 276
PsuI RGATCY 1 cut(s) 1015
PvuI CGATCG 1 cut(s) 393
RsaI GTAC 4 cut(s) 35, 165, 216, 466
RsaNI GTAC 4 cut(s) 34, 164, 215, 465
RseI CAYNNNNRTG 3 cut(s) 222, 762, 829
SaqAI TTAA 8 cut(s) 27, 293, 548, 561, 567, 590, 729, 735
SatI GCNGC 1 cut(s) 901
Sau3AI GATC 5 cut(s) 199, 386, 390, 652, 1015
Sau96I GGNCC 1 cut(s) 340
ScrFI CCNGG 2 cut(s) 53, 612
SduI GDGCHC 1 cut(s) 969
Sfr274I CTCGAG 1 cut(s) 796
SinI GGWCC 1 cut(s) 340
SlaI CTCGAG 1 cut(s) 796
SmiMI CAYNNNNRTG 3 cut(s) 222, 762, 829
SmlI CTYRAG 1 cut(s) 796
SmoI CTYRAG 1 cut(s) 796
Sse9I AATT 5 cut(s) 21, 294, 311, 469, 732
SsiI CCGC 5 cut(s) 38, 494, 903, 953, 995
SspI AATATT 3 cut(s) 512, 534, 576
SspMI CTAG 1 cut(s) 650
StyD4I CCNGG 2 cut(s) 51, 610
StyI CCWWGG 1 cut(s) 630
TaaI ACNGT 3 cut(s) 279, 948, 971
TaiI ACGT 7 cut(s) 35, 250, 293, 384, 620, 741, 982
TaqI TCGA 5 cut(s) 202, 250, 389, 475, 797
TasI AATT 5 cut(s) 21, 294, 311, 469, 732
TatI WGTACW 1 cut(s) 214
TfiI GAWTC 2 cut(s) 207, 554
Tru1I TTAA 8 cut(s) 27, 293, 548, 561, 567, 590, 729, 735
Tru9I TTAA 8 cut(s) 27, 293, 548, 561, 567, 590, 729, 735
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 2 cut(s) 273, 791
TseI GCWGC 1 cut(s) 900
Tsp45I GTSAC 2 cut(s) 273, 791
TspDTI ATGAA 5 cut(s) 212, 271, 446, 660, 697
TspGWI ACGGA 5 cut(s) 181, 505, 521, 566, 585
TspRI CASTG 1 cut(s) 282
Van91I CCANNNNNTGG 1 cut(s) 642
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 2 cut(s) 21, 311
XceI RCATGY 1 cut(s) 610
XhoI CTCGAG 1 cut(s) 796
XspI CTAG 1 cut(s) 650
ZraI GACGTC 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.