pycom04g16620

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0000513
Physical Location & Seq
Reverse (-)
19291271 .. 19292583
1313 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16620.3

Sequence Viewer

Length: 510 bp
ATGACGATTTTGCCACTAGCATGTTGGTTTCGTTGTGTGGTTGTTTTGGGTTTTGTTTGGCTGGTTTTGGACCACACACACCTTCCCACATCCCTTCAACCCTTTCCCTGCCCTGTTCCCTGTCTCTTTCTCTATCTCTCTCCCATTTCTATCCTCCCATGTACGGACATAATCAAAACCATCCAAACCTTCACAGATCGAGGAAACAAATTACATATTCGTGCTCGTGAGGTCCGTAGGAGTTCAACCATACCTATTTCAGCTTGTAGGAAACTTGGTGAGGTCCTTAGGAGGCTCGGGGTGGTTTGTTTGAAGGTTTTGGACGTCGGGATCGTGAGTTACAAGCTTGGCCGGAGTTGGTGGTGTTTTCCCGGCGAGATTCCGTGGATTTTAGTGCTTGAAAGTGGTGAGACCTATCCCAAGGACGAGCGCGGTCACTCGAGGCAAGGGGGTTACGACCCTTCTACATACCATGCTCCCGCCCAGAGTTGGGCACAGTCCTTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

19.18

Weight (kDa)

9.0

Isoelectric Point (pI)

64.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 327
AccII CGCG 1 cut(s) 432
AciI CCGC 2 cut(s) 432, 480
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 349
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 1 cut(s) 163
AfiI CCNNNNNNNGG 3 cut(s) 163, 489, 490
AgsI TTSAA 4 cut(s) 98, 246, 313, 401
AluBI AGCT 2 cut(s) 263, 346
AluI AGCT 2 cut(s) 263, 346
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 2 cut(s) 128, 404
AlwI GGATC 1 cut(s) 338
Ama87I CYCGRG 2 cut(s) 296, 439
AoxI GGCC 1 cut(s) 349
AspLEI GCGC 1 cut(s) 432
AspS9I GGNCC 3 cut(s) 70, 232, 283
AsuC2I CCSGG 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 290, 419
AvaI CYCGRG 2 cut(s) 296, 439
AvaII GGWCC 3 cut(s) 70, 232, 283
AxyI CCTNAGG 1 cut(s) 287
BaeGI GKGCMC 1 cut(s) 496
BauI CACGAG 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 226
BccI CCATC 1 cut(s) 188
BcnI CCSGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 128, 404
BfaI CTAG 1 cut(s) 17
Bme1390I CCNGG 1 cut(s) 372
Bme18I GGWCC 3 cut(s) 70, 232, 283
BmeT110I CYCGRG 2 cut(s) 296, 439
BmgT120I GGNCC 3 cut(s) 70, 232, 283
BmrFI CCNGG 1 cut(s) 372
BpuMI CCSGG 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 383, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 489, 490
Bse21I CCTNAGG 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 383, 420
BseGI GGATG 2 cut(s) 89, 180
BseLI CCNNNNNNNGG 3 cut(s) 163, 489, 490
BseSI GKGCMC 1 cut(s) 496
Bsh1236I CGCG 1 cut(s) 432
BshFI GGCC 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 226
BsiHKCI CYCGRG 2 cut(s) 296, 439
BsiSI CCGG 2 cut(s) 352, 372
BslI CCNNNNNNNGG 3 cut(s) 163, 489, 490
BsmAI GTCTC 2 cut(s) 128, 404
BsnI GGCC 1 cut(s) 351
Bso31I GGTCTC 1 cut(s) 404
BsoBI CYCGRG 2 cut(s) 296, 439
Bsp1286I GDGCHC 2 cut(s) 226, 496
Bsp143I GATC 2 cut(s) 196, 330
BspACI CCGC 2 cut(s) 432, 480
BspANI GGCC 1 cut(s) 351
BspFNI CGCG 1 cut(s) 432
BspPI GGATC 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 404
BssECI CCNNGG 2 cut(s) 383, 420
BssMI GATC 2 cut(s) 196, 330
BssNI GRCGYC 1 cut(s) 324
BssSI CACGAG 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 420
Bst2BI CACGAG 1 cut(s) 225
Bst4CI ACNGT 1 cut(s) 498
BstACI GRCGYC 1 cut(s) 324
BstDEI CTNAG 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 383
BstF5I GGATG 2 cut(s) 89, 180
BstFNI CGCG 1 cut(s) 432
BstHHI GCGC 1 cut(s) 432
BstKTI GATC 2 cut(s) 199, 333
BstMAI GTCTC 2 cut(s) 128, 404
BstMBI GATC 2 cut(s) 196, 330
BstNSI RCATGY 1 cut(s) 24
BstSCI CCNGG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 496
BstUI CGCG 1 cut(s) 432
Bsu36I CCTNAGG 1 cut(s) 287
BsuRI GGCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 383
BtsCI GGATG 2 cut(s) 89, 180
CfoI GCGC 1 cut(s) 432
Cfr13I GGNCC 3 cut(s) 70, 232, 283
Csp6I GTAC 1 cut(s) 162
CviAII CATG 4 cut(s) 21, 159, 473, 505
CviJI RGCY 5 cut(s) 61, 263, 295, 346, 351
CviKI_1 RGCY 5 cut(s) 61, 263, 295, 346, 351
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 1 cut(s) 287
DpnI GATC 2 cut(s) 198, 332
DpnII GATC 2 cut(s) 196, 330
EaeI YGGCCR 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 420
Eco31I GGTCTC 1 cut(s) 404
Eco47I GGWCC 3 cut(s) 70, 232, 283
Eco81I CCTNAGG 1 cut(s) 287
Eco88I CYCGRG 2 cut(s) 296, 439
EcoO109I RGGNCCY 1 cut(s) 283
EcoT14I CCWWGG 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 4 cut(s) 24, 162, 476, 508
FaiI YATR 8 cut(s) 22, 160, 170, 216, 251, 469, 474, 506
FatI CATG 4 cut(s) 20, 158, 472, 504
FauI CCCGC 1 cut(s) 487
FokI GGATG 2 cut(s) 76, 167
FspBI CTAG 1 cut(s) 17
GlaI GCGC 1 cut(s) 431
HaeIII GGCC 1 cut(s) 351
HapII CCGG 2 cut(s) 352, 372
HhaI GCGC 1 cut(s) 432
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 4 cut(s) 24, 162, 476, 508
Hin6I GCGC 1 cut(s) 430
HinP1I GCGC 1 cut(s) 430
HindIII AAGCTT 1 cut(s) 344
HinfI GANTC 1 cut(s) 379
HpaII CCGG 2 cut(s) 352, 372
HphI GGTGA 2 cut(s) 290, 419
Hpy188III TCNNGA 3 cut(s) 227, 328, 334
Hpy99I CGWCG 1 cut(s) 329
HpyAV CCTTC 5 cut(s) 92, 104, 199, 307, 471
HpyCH4III ACNGT 1 cut(s) 498
HpyCH4IV ACGT 1 cut(s) 324
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 1 cut(s) 324
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 4 cut(s) 24, 162, 476, 508
HspAI GCGC 1 cut(s) 430
Kzo9I GATC 2 cut(s) 196, 330
LmnI GCTCC 1 cut(s) 481
LpnPI CCDG 7 cut(s) 47, 121, 126, 133, 365, 385, 497
MaeI CTAG 1 cut(s) 17
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 3 cut(s) 338, 434, 452
MalI GATC 2 cut(s) 198, 332
MboI GATC 2 cut(s) 196, 330
MhlI GDGCHC 2 cut(s) 226, 496
MluCI AATT 1 cut(s) 209
MnlI CCTC 6 cut(s) 164, 194, 223, 274, 285, 435
MslI CAYNNNNRTG 2 cut(s) 19, 219
MspI CCGG 2 cut(s) 352, 372
MspR9I CCNGG 1 cut(s) 372
MvnI CGCG 1 cut(s) 432
NciI CCSGG 1 cut(s) 372
NdeII GATC 2 cut(s) 196, 330
NlaIII CATG 4 cut(s) 24, 162, 476, 508
NmuCI GTSAC 1 cut(s) 434
NspI RCATGY 1 cut(s) 24
PaeR7I CTCGAG 1 cut(s) 439
PcsI WCGNNNNNNNCGW 2 cut(s) 232, 330
PfeI GAWTC 1 cut(s) 379
PpuMI RGGWCCY 1 cut(s) 283
Psp5II RGGWCCY 1 cut(s) 283
PspPI GGNCC 3 cut(s) 70, 232, 283
PspPPI RGGWCCY 1 cut(s) 283
PspXI VCTCGAGB 1 cut(s) 439
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
RseI CAYNNNNRTG 2 cut(s) 19, 219
Sau3AI GATC 2 cut(s) 196, 330
Sau96I GGNCC 3 cut(s) 70, 232, 283
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 2 cut(s) 226, 496
Sfr274I CTCGAG 1 cut(s) 439
SinI GGWCC 3 cut(s) 70, 232, 283
SlaI CTCGAG 1 cut(s) 439
SmiMI CAYNNNNRTG 2 cut(s) 19, 219
SmlI CTYRAG 1 cut(s) 439
SmoI CTYRAG 1 cut(s) 439
Sse9I AATT 1 cut(s) 209
SsiI CCGC 2 cut(s) 432, 480
SspMI CTAG 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 370
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 498
TaiI ACGT 1 cut(s) 327
TaqI TCGA 2 cut(s) 199, 440
TasI AATT 1 cut(s) 209
TfiI GAWTC 1 cut(s) 379
TseFI GTSAC 1 cut(s) 434
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 1 cut(s) 493
TspGWI ACGGA 3 cut(s) 179, 224, 372
VpaK11BI GGWCC 3 cut(s) 70, 232, 283
XceI RCATGY 1 cut(s) 24
XcmI CCANNNNNNNNNTGG 1 cut(s) 21
XhoI CTCGAG 1 cut(s) 439
XspI CTAG 1 cut(s) 17
ZraI GACGTC 1 cut(s) 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.