pycom05g22750

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000513
Physical Location & Seq
Forward (+)
24951218 .. 24952964
1747 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g22750.3

Sequence Viewer

Length: 1266 bp
ATGGCATGTGGCCGCGCGTGGGGGCGCGTAGGTCCGTGCCTATTTTGGCGCGTGGGGGCGCGTGAGGGGTCCAAAAATTATTTTAAAAATATGGTTGTGATCCTGAGGTTGTGTAGAGCACGTTGGTATATTCATTTGTCCATTTTGACTATTTCGCATATAGGTGACACGTACCCCGAGGACGAGCGTGCACACGCGAGGCAGGGGGGCTACGACCCTTCTACATACCAAAATGTTTATTCATTATGTCATGCATTACATTATTGTTTTTCGTGCATCGTTGGGTACGTTATTAGTTGCATATATTTATACATATGCATATTGGGTGCTGTGGACGCACATGTAAGTGCCAGAGTTAGAGAGCTCATAACCTGCACCCCCGGTGTTAGTGCTCCCGCCCGTGGCCAGGGTACAGTCCTTCACGTGATGTTCACCTCCCGCACCTTATACTCACCTTGGATTCAAGGTAGGTGCACAGTGACCCGCGATTATTCGCCCAGTCTTCACGTGATCGTAGCACTTATTTACACCCAGGCCTGTCGTACAGACCACTTTAGGTGGTTCCGACTCGTGGCCGTACAGGTCACGTTAGGTGACTTCGGCTGCCAGATTACATTTATACCTGGGCACATGCATATCATTATGAGATTTCGGCATGGCATATTATTTGAGCATGTTTGGCATATGTATTATGTGTATATATCTTCTGGGAAGTATACAGGTTTTACGCTTTGTTTTTGCCCACTCACTTCGGTGGTTTTCCCAGAGGATTCTCCCGGCTTTCTGACAGATACTCACCAGCGTAGGACCTGCTCTGATTTTGTATTTCACTTACTAGCACTTGTCTTAACACTTTGTATGCTAGTTTTTAATTTATTCGTACTTTTATATTACTACTTTATTAGCTTCCGCACGTGCACATGGCTACGTCACCTTCGTGTGACGGCCAGCACGCTCCGATCTCGGTCGGGGTGTGTCAGTTTGGTATCAGAGCCAATCCCTGGTCGGAAGTGTGCTGACGAGGACGTCGGGCCCCTAAGGGGGGTGGATTGTGACATCCCACATCGCCCAGGGGACTTCGGGTTCCGTAGGAACTCCGAAGTTAAGCGAGAAGGGGGCTTGAGCAATCCCATGATGGGTGACCCACTGGGAAGTTCCCAAAGCGGACAATATCGTGCTACGGTAGTGGAGCGGGCTCGGGAAGTGATCCGTCCCGGGCCTGGATGTGACATGATTATAAAAAATATAATATCAATTTATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

422

Amino Acids

47.83

Weight (kDa)

9.0

Isoelectric Point (pI)

45.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1238
AasI GACNNNNNNGTC 1 cut(s) 1025
AatII GACGTC 1 cut(s) 1029
Acc36I ACCTGC 2 cut(s) 380, 818
AccB7I CCANNNNNTGG 1 cut(s) 1001
AccBSI CCGCTC 1 cut(s) 1192
AccI GTMKAC 1 cut(s) 716
AccII CGCG 7 cut(s) 15, 17, 27, 51, 61, 197, 486
AciI CCGC 7 cut(s) 13, 396, 439, 484, 910, 1164, 1192
AclWI GGATC 2 cut(s) 94, 1201
AcoI YGGCCR 4 cut(s) 10, 403, 573, 945
AcvI CACGTG 3 cut(s) 424, 508, 915
AcyI GRCGYC 1 cut(s) 1026
AfaI GTAC 6 cut(s) 173, 287, 412, 544, 579, 882
AflIII ACRYGT 2 cut(s) 168, 340
AgsI TTSAA 1 cut(s) 464
AjnI CCWGG 6 cut(s) 405, 531, 622, 1000, 1069, 1219
AleI CACNNNNGTG 2 cut(s) 752, 936
AloI GAACNNNNNNTCC 2 cut(s) 1067, 1099
AluBI AGCT 2 cut(s) 364, 906
AluI AGCT 2 cut(s) 364, 906
Alw21I GWGCWC 6 cut(s) 121, 193, 366, 394, 476, 920
Alw44I GTGCAC 3 cut(s) 189, 472, 916
AlwI GGATC 2 cut(s) 94, 1201
Ama87I CYCGRG 3 cut(s) 176, 1197, 1214
AoxI GGCC 7 cut(s) 10, 403, 534, 573, 945, 1031, 1217
ApaI GGGCCC 1 cut(s) 1035
ApaLI GTGCAC 3 cut(s) 189, 472, 916
ApeKI GCWGC 1 cut(s) 603
AspLEI GCGC 4 cut(s) 17, 27, 51, 61
AspS9I GGNCC 6 cut(s) 32, 69, 807, 1031, 1032, 1217
AsuC2I CCSGG 4 cut(s) 381, 777, 1215, 1216
AsuHPI GGTGA 7 cut(s) 176, 424, 444, 605, 788, 923, 1151
AvaI CYCGRG 3 cut(s) 176, 1197, 1214
AvaII GGWCC 3 cut(s) 32, 69, 807
AxyI CCTNAGG 2 cut(s) 104, 1037
BaeGI GKGCMC 5 cut(s) 193, 476, 630, 920, 1035
BalI TGGCCA 1 cut(s) 405
BanII GRGCYC 3 cut(s) 366, 1035, 1198
BauI CACGAG 1 cut(s) 569
BbrPI CACGTG 3 cut(s) 424, 508, 915
BbsI GAAGAC 1 cut(s) 494
Bbv12I GWGCWC 6 cut(s) 121, 193, 366, 394, 476, 920
BbvI GCAGC 1 cut(s) 590
BccI CCATC 1 cut(s) 1129
BceAI ACGGC 2 cut(s) 560, 960
BciT130I CCWGG 6 cut(s) 407, 533, 624, 1002, 1071, 1221
BcnI CCSGG 4 cut(s) 381, 777, 1215, 1216
BfaI CTAG 2 cut(s) 836, 863
BfuAI ACCTGC 2 cut(s) 380, 818
BisI GCNGC 2 cut(s) 13, 604
BlsI GCNGC 2 cut(s) 14, 605
Bme18I GGWCC 3 cut(s) 32, 69, 807
BmeT110I CYCGRG 3 cut(s) 176, 1197, 1214
BmgT120I GGNCC 6 cut(s) 32, 69, 807, 1031, 1032, 1217
BmiI GGNNCC 5 cut(s) 70, 563, 1033, 1034, 1085
BmrI ACTGGG 2 cut(s) 492, 1157
BmsI GCATC 1 cut(s) 285
BmuI ACTGGG 2 cut(s) 492, 1157
BpiI GAAGAC 1 cut(s) 494
BpuEI CTTGAG 1 cut(s) 1141
BpuMI CCSGG 4 cut(s) 381, 777, 1215, 1216
BsaAI YACGTR 4 cut(s) 171, 424, 508, 915
BsaHI GRCGYC 1 cut(s) 1026
Bse1I ACTGG 2 cut(s) 498, 1152
Bse21I CCTNAGG 2 cut(s) 104, 1037
BseBI CCWGG 6 cut(s) 407, 533, 624, 1002, 1071, 1221
BseGI GGATG 2 cut(s) 1056, 1229
BseMII CTCAG 1 cut(s) 95
BseNI ACTGG 2 cut(s) 498, 1152
BseSI GKGCMC 5 cut(s) 193, 476, 630, 920, 1035
BseXI GCAGC 1 cut(s) 590
BsgI GTGCAG 1 cut(s) 358
Bsh1236I CGCG 7 cut(s) 15, 17, 27, 51, 61, 197, 486
Bsh1285I CGRYCG 1 cut(s) 968
BshFI GGCC 7 cut(s) 12, 405, 536, 575, 947, 1033, 1219
BsiEI CGRYCG 1 cut(s) 968
BsiHKAI GWGCWC 6 cut(s) 121, 193, 366, 394, 476, 920
BsiHKCI CYCGRG 3 cut(s) 176, 1197, 1214
BsiSI CCGG 3 cut(s) 381, 777, 1215
BslFI GGGAC 2 cut(s) 1088, 1197
BsmFI GGGAC 2 cut(s) 1088, 1197
BsnI GGCC 7 cut(s) 12, 405, 536, 575, 947, 1033, 1219
BsoBI CYCGRG 3 cut(s) 176, 1197, 1214
Bsp120I GGGCCC 1 cut(s) 1031
Bsp1286I GDGCHC 9 cut(s) 121, 193, 366, 394, 476, 630, 920, 1035, 1198
Bsp143I GATC 4 cut(s) 99, 510, 959, 1206
BspACI CCGC 7 cut(s) 13, 396, 439, 484, 910, 1164, 1192
BspANI GGCC 7 cut(s) 12, 405, 536, 575, 947, 1033, 1219
BspCNI CTCAG 1 cut(s) 96
BspFNI CGCG 7 cut(s) 15, 17, 27, 51, 61, 197, 486
BspLI GGNNCC 5 cut(s) 70, 563, 1033, 1034, 1085
BspMI ACCTGC 2 cut(s) 380, 818
BspPI GGATC 2 cut(s) 94, 1201
BsrBI CCGCTC 1 cut(s) 1192
BsrI ACTGG 2 cut(s) 498, 1152
BssMI GATC 4 cut(s) 99, 510, 959, 1206
BssNAI GTATAC 1 cut(s) 717
BssNI GRCGYC 1 cut(s) 1026
BssSI CACGAG 1 cut(s) 569
BssT1I CCWWGG 1 cut(s) 455
Bst1107I GTATAC 1 cut(s) 717
Bst2BI CACGAG 1 cut(s) 569
Bst2UI CCWGG 6 cut(s) 407, 533, 624, 1002, 1071, 1221
Bst4CI ACNGT 3 cut(s) 415, 478, 1183
BstACI GRCGYC 1 cut(s) 1026
BstBAI YACGTR 4 cut(s) 171, 424, 508, 915
BstC8I GCNNGC 4 cut(s) 189, 949, 953, 1194
BstDEI CTNAG 2 cut(s) 104, 1037
BstDSI CCRYGG 1 cut(s) 400
BstEII GGTNACC 1 cut(s) 1139
BstF5I GGATG 2 cut(s) 1056, 1229
BstFNI CGCG 7 cut(s) 15, 17, 27, 51, 61, 197, 486
BstHHI GCGC 4 cut(s) 17, 27, 51, 61
BstKTI GATC 4 cut(s) 102, 513, 962, 1209
BstMBI GATC 4 cut(s) 99, 510, 959, 1206
BstMCI CGRYCG 1 cut(s) 968
BstMWI GCNNNNNNNGC 2 cut(s) 335, 679
BstNI CCWGG 6 cut(s) 407, 533, 624, 1002, 1071, 1221
BstNSI RCATGY 4 cut(s) 9, 344, 634, 677
BstPI GGTNACC 1 cut(s) 1139
BstSLI GKGCMC 5 cut(s) 193, 476, 630, 920, 1035
BstUI CGCG 7 cut(s) 15, 17, 27, 51, 61, 197, 486
BstV1I GCAGC 1 cut(s) 590
BstV2I GAAGAC 1 cut(s) 494
BstZ17I GTATAC 1 cut(s) 717
Bsu36I CCTNAGG 2 cut(s) 104, 1037
BsuRI GGCC 7 cut(s) 12, 405, 536, 575, 947, 1033, 1219
BtgI CCRYGG 1 cut(s) 400
BtgZI GCGATG 1 cut(s) 1049
BtsCI GGATG 2 cut(s) 1056, 1229
BtsIMutI CAGTG 2 cut(s) 483, 1145
BveI ACCTGC 2 cut(s) 380, 818
Cac8I GCNNGC 4 cut(s) 189, 949, 953, 1194
CfoI GCGC 4 cut(s) 17, 27, 51, 61
Cfr13I GGNCC 6 cut(s) 32, 69, 807, 1031, 1032, 1217
Cfr9I CCCGGG 1 cut(s) 1214
CseI GACGC 1 cut(s) 344
Csp6I GTAC 6 cut(s) 172, 286, 411, 543, 578, 881
CviAII CATG 9 cut(s) 6, 251, 341, 631, 656, 674, 921, 1132, 1231
CviQI GTAC 6 cut(s) 172, 286, 411, 543, 578, 881
DdeI CTNAG 2 cut(s) 104, 1037
DpnI GATC 4 cut(s) 101, 512, 961, 1208
DpnII GATC 4 cut(s) 99, 510, 959, 1206
DraI TTTAAA 1 cut(s) 85
DrdI GACNNNNNNGTC 1 cut(s) 1025
DseDI GACNNNNNNGTC 1 cut(s) 1025
EaeI YGGCCR 4 cut(s) 10, 403, 573, 945
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 455
Eco147I AGGCCT 1 cut(s) 536
Eco24I GRGCYC 3 cut(s) 366, 1035, 1198
Eco47I GGWCC 3 cut(s) 32, 69, 807
Eco53kI GAGCTC 1 cut(s) 364
Eco72I CACGTG 3 cut(s) 424, 508, 915
Eco81I CCTNAGG 2 cut(s) 104, 1037
Eco88I CYCGRG 3 cut(s) 176, 1197, 1214
Eco91I GGTNACC 1 cut(s) 1139
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 2 cut(s) 807, 1032
EcoO65I GGTNACC 1 cut(s) 1139
EcoRII CCWGG 6 cut(s) 405, 531, 622, 1000, 1069, 1219
EcoT14I CCWWGG 1 cut(s) 455
EcoT22I ATGCAT 3 cut(s) 256, 320, 636
EcoT38I GRGCYC 3 cut(s) 366, 1035, 1198
ErhI CCWWGG 1 cut(s) 455
FaeI CATG 9 cut(s) 9, 254, 344, 634, 659, 677, 924, 1135, 1234
FaqI GGGAC 2 cut(s) 1088, 1197
FatI CATG 9 cut(s) 5, 250, 340, 630, 655, 673, 920, 1131, 1230
FauI CCCGC 4 cut(s) 403, 446, 491, 1185
FauNDI CATATG 2 cut(s) 314, 684
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 2 cut(s) 13, 604
FokI GGATG 2 cut(s) 1043, 1236
FriOI GRGCYC 3 cut(s) 366, 1035, 1198
Fsp4HI GCNGC 2 cut(s) 13, 604
FspBI CTAG 2 cut(s) 836, 863
GlaI GCGC 4 cut(s) 16, 26, 50, 60
GluI GCNGC 2 cut(s) 13, 604
HaeIII GGCC 7 cut(s) 12, 405, 536, 575, 947, 1033, 1219
HapII CCGG 3 cut(s) 381, 777, 1215
HgaI GACGC 1 cut(s) 344
HhaI GCGC 4 cut(s) 17, 27, 51, 61
Hin1I GRCGYC 1 cut(s) 1026
Hin1II CATG 9 cut(s) 9, 254, 344, 634, 659, 677, 924, 1135, 1234
Hin6I GCGC 4 cut(s) 15, 25, 49, 59
HinP1I GCGC 4 cut(s) 15, 25, 49, 59
HinfI GANTC 3 cut(s) 460, 567, 770
HpaII CCGG 3 cut(s) 381, 777, 1215
HphI GGTGA 7 cut(s) 176, 424, 444, 605, 788, 923, 1151
Hpy166II GTNNAC 6 cut(s) 191, 334, 432, 474, 717, 918
Hpy188I TCNGA 7 cut(s) 566, 786, 817, 959, 991, 1008, 1099
Hpy188III TCNNGA 2 cut(s) 103, 1199
Hpy8I GTNNAC 6 cut(s) 191, 334, 432, 474, 717, 918
Hpy99I CGWCG 1 cut(s) 1031
HpyAV CCTTC 4 cut(s) 228, 428, 944, 1106
HpyCH4III ACNGT 3 cut(s) 415, 478, 1183
HpyCH4IV ACGT 9 cut(s) 121, 170, 288, 423, 507, 587, 914, 928, 1026
HpyCH4V TGCA 9 cut(s) 191, 254, 276, 300, 318, 375, 474, 634, 918
HpyF10VI GCNNNNNNNGC 2 cut(s) 335, 679
HpyF3I CTNAG 2 cut(s) 104, 1037
HpySE526I ACGT 9 cut(s) 121, 170, 288, 423, 507, 587, 914, 928, 1026
Hsp92I GRCGYC 1 cut(s) 1026
Hsp92II CATG 9 cut(s) 9, 254, 344, 634, 659, 677, 924, 1135, 1234
HspAI GCGC 4 cut(s) 15, 25, 49, 59
Kzo9I GATC 4 cut(s) 99, 510, 959, 1206
LmnI GCTCC 3 cut(s) 397, 960, 1189
Lsp1109I GCAGC 1 cut(s) 590
LweI GCATC 1 cut(s) 285
MaeI CTAG 2 cut(s) 836, 863
MaeII ACGT 9 cut(s) 121, 170, 288, 423, 507, 587, 914, 928, 1026
MaeIII GTNAC 9 cut(s) 164, 478, 583, 593, 929, 940, 1052, 1139, 1226
MalI GATC 4 cut(s) 101, 512, 961, 1208
MbiI CCGCTC 1 cut(s) 1192
MboI GATC 4 cut(s) 99, 510, 959, 1206
MboII GAAGA 2 cut(s) 494, 696
MhlI GDGCHC 9 cut(s) 121, 193, 366, 394, 476, 630, 920, 1035, 1198
MlsI TGGCCA 1 cut(s) 405
MluCI AATT 3 cut(s) 76, 871, 1254
MluNI TGGCCA 1 cut(s) 405
MlyI GAGTC 1 cut(s) 561
MmeI TCCRAC 2 cut(s) 589, 986
MnlI CCTC 7 cut(s) 58, 99, 172, 192, 445, 760, 1015
Mox20I TGGCCA 1 cut(s) 405
Mph1103I ATGCAT 3 cut(s) 256, 320, 636
MscI TGGCCA 1 cut(s) 405
MseI TTAA 4 cut(s) 84, 848, 870, 1104
MslI CAYNNNNRTG 4 cut(s) 162, 345, 752, 936
Msp20I TGGCCA 1 cut(s) 405
MspI CCGG 3 cut(s) 381, 777, 1215
MvaI CCWGG 6 cut(s) 407, 533, 624, 1002, 1071, 1221
MvnI CGCG 7 cut(s) 15, 17, 27, 51, 61, 197, 486
MwoI GCNNNNNNNGC 2 cut(s) 335, 679
NciI CCSGG 4 cut(s) 381, 777, 1215, 1216
NdeI CATATG 2 cut(s) 314, 684
NdeII GATC 4 cut(s) 99, 510, 959, 1206
NlaIII CATG 9 cut(s) 9, 254, 344, 634, 659, 677, 924, 1135, 1234
NlaIV GGNNCC 5 cut(s) 70, 563, 1033, 1034, 1085
NmuCI GTSAC 9 cut(s) 164, 478, 583, 593, 929, 940, 1052, 1139, 1226
NsiI ATGCAT 3 cut(s) 256, 320, 636
NspI RCATGY 4 cut(s) 9, 344, 634, 677
OliI CACNNNNGTG 2 cut(s) 752, 936
PasI CCCWGGG 1 cut(s) 1070
PceI AGGCCT 1 cut(s) 536
PciI ACATGT 1 cut(s) 340
PcsI WCGNNNNNNNCGW 2 cut(s) 285, 934
PfeI GAWTC 2 cut(s) 460, 770
PflMI CCANNNNNTGG 1 cut(s) 1001
PkrI GCNGC 2 cut(s) 14, 605
PleI GAGTC 1 cut(s) 561
PmaCI CACGTG 3 cut(s) 424, 508, 915
PmlI CACGTG 3 cut(s) 424, 508, 915
PpsI GAGTC 1 cut(s) 561
Ppu21I YACGTR 4 cut(s) 171, 424, 508, 915
PpuMI RGGWCCY 1 cut(s) 807
PscI ACATGT 1 cut(s) 340
PsiI TTATAA 1 cut(s) 1238
Psp124BI GAGCTC 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 807
Psp6I CCWGG 6 cut(s) 405, 531, 622, 1000, 1069, 1219
PspCI CACGTG 3 cut(s) 424, 508, 915
PspEI GGTNACC 1 cut(s) 1139
PspGI CCWGG 6 cut(s) 405, 531, 622, 1000, 1069, 1219
PspN4I GGNNCC 5 cut(s) 70, 563, 1033, 1034, 1085
PspOMI GGGCCC 1 cut(s) 1031
PspPI GGNCC 6 cut(s) 32, 69, 807, 1031, 1032, 1217
PspPPI RGGWCCY 1 cut(s) 807
RsaI GTAC 6 cut(s) 173, 287, 412, 544, 579, 882
RsaNI GTAC 6 cut(s) 172, 286, 411, 543, 578, 881
RseI CAYNNNNRTG 4 cut(s) 162, 345, 752, 936
SacI GAGCTC 1 cut(s) 366
SaqAI TTAA 4 cut(s) 84, 848, 870, 1104
SatI GCNGC 2 cut(s) 13, 604
Sau3AI GATC 4 cut(s) 99, 510, 959, 1206
Sau96I GGNCC 6 cut(s) 32, 69, 807, 1031, 1032, 1217
SchI GAGTC 1 cut(s) 561
SduI GDGCHC 9 cut(s) 121, 193, 366, 394, 476, 630, 920, 1035, 1198
SfaNI GCATC 1 cut(s) 285
SinI GGWCC 3 cut(s) 32, 69, 807
SmaI CCCGGG 1 cut(s) 1216
SmiMI CAYNNNNRTG 4 cut(s) 162, 345, 752, 936
SmlI CTYRAG 1 cut(s) 1120
SmoI CTYRAG 1 cut(s) 1120
Sse9I AATT 3 cut(s) 76, 871, 1254
SseBI AGGCCT 1 cut(s) 536
SsiI CCGC 7 cut(s) 13, 396, 439, 484, 910, 1164, 1192
SspMI CTAG 2 cut(s) 836, 863
SstI GAGCTC 1 cut(s) 366
StuI AGGCCT 1 cut(s) 536
StyI CCWWGG 1 cut(s) 455
TaaI ACNGT 3 cut(s) 415, 478, 1183
TaiI ACGT 9 cut(s) 124, 173, 291, 426, 510, 590, 917, 931, 1029
TaqII GACCGA 1 cut(s) 954
TasI AATT 3 cut(s) 76, 871, 1254
TauI GCSGC 1 cut(s) 15
TfiI GAWTC 2 cut(s) 460, 770
Tru1I TTAA 4 cut(s) 84, 848, 870, 1104
Tru9I TTAA 4 cut(s) 84, 848, 870, 1104
TscAI CASTG 2 cut(s) 483, 1152
TseFI GTSAC 9 cut(s) 164, 478, 583, 593, 929, 940, 1052, 1139, 1226
TseI GCWGC 1 cut(s) 603
Tsp45I GTSAC 9 cut(s) 164, 478, 583, 593, 929, 940, 1052, 1139, 1226
TspDTI ATGAA 2 cut(s) 122, 231
TspGWI ACGGA 3 cut(s) 24, 1076, 1199
TspMI CCCGGG 1 cut(s) 1214
TspRI CASTG 2 cut(s) 483, 1152
Van91I CCANNNNNTGG 1 cut(s) 1001
VneI GTGCAC 3 cut(s) 189, 472, 916
VpaK11BI GGWCC 3 cut(s) 32, 69, 807
XceI RCATGY 4 cut(s) 9, 344, 634, 677
XmaI CCCGGG 1 cut(s) 1214
XmiI GTMKAC 1 cut(s) 716
XspI CTAG 2 cut(s) 836, 863
ZraI GACGTC 1 cut(s) 1027
Zsp2I ATGCAT 3 cut(s) 256, 320, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.