pycom16g21190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000513
Physical Location & Seq
Forward (+)
19436157 .. 19437142
986 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g21190.3

Sequence Viewer

Length: 426 bp
ATGTTTTTGGGTTTTTGTTGGCATGTTTTGGGCCACACACACTCTCCTACACCGTACACCATTTTCCCTATCCCTGCACCCTGTCCCGAGCTCCCTTCCCTTCCCATTTCCTACCCAGGTGTACGGACATACACACAAACCCATCAAACCTTCACAGATCGAAGAAATAAAGTACATATCCATGCTCGTGAGGTCCATTGGAGTCTAACCATACCCATTTCAGTTCAAGCTTGTAGGAAGCTTGGTGAGGTCCTTAGGAGGCTCGGGGTGGTTCGTTTGAAAGTTTTGGACGTCGGGATCACGAGTTTCAAGGTTGGCCGGAGTTGGGGGTATTTTCCAGTTGTTTCGCATATAGGTGATACCTATCCCGAGGACGAGCGTGGTCACTCGAGGCAGGGGGGTTACGACCCTTCCACTTACCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

16.05

Weight (kDa)

9.33

Isoelectric Point (pI)

53.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 294
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 1 cut(s) 316
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 3 cut(s) 56, 123, 174
AfiI CCNNNNNNNGG 1 cut(s) 325
AgsI TTSAA 3 cut(s) 227, 280, 310
AjnI CCWGG 1 cut(s) 115
AluBI AGCT 3 cut(s) 91, 230, 241
AluI AGCT 3 cut(s) 91, 230, 241
Alw21I GWGCWC 1 cut(s) 93
AlwI GGATC 1 cut(s) 305
Ama87I CYCGRG 4 cut(s) 86, 263, 368, 388
AoxI GGCC 2 cut(s) 31, 316
AspS9I GGNCC 3 cut(s) 31, 193, 250
AsuHPI GGTGA 2 cut(s) 257, 368
AvaI CYCGRG 4 cut(s) 86, 263, 368, 388
AvaII GGWCC 2 cut(s) 193, 250
AxyI CCTNAGG 1 cut(s) 254
BanII GRGCYC 1 cut(s) 93
BauI CACGAG 2 cut(s) 186, 301
Bbv12I GWGCWC 1 cut(s) 93
BccI CCATC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 117
Bme1390I CCNGG 1 cut(s) 117
Bme18I GGWCC 2 cut(s) 193, 250
BmeT110I CYCGRG 4 cut(s) 86, 263, 368, 388
BmgT120I GGNCC 3 cut(s) 31, 193, 250
BmrFI CCNGG 1 cut(s) 117
BsaBI GATNNNNATC 1 cut(s) 363
BsaHI GRCGYC 1 cut(s) 291
BsaJI CCNNGG 2 cut(s) 115, 369
BsaXI ACNNNNNCTCC 2 cut(s) 28, 58
Bsc4I CCNNNNNNNGG 1 cut(s) 325
Bse1I ACTGG 1 cut(s) 338
Bse21I CCTNAGG 1 cut(s) 254
Bse8I GATNNNNATC 1 cut(s) 363
BseBI CCWGG 1 cut(s) 117
BseDI CCNNGG 2 cut(s) 115, 369
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 1 cut(s) 325
BseNI ACTGG 1 cut(s) 338
BsgI GTGCAG 1 cut(s) 60
BshFI GGCC 2 cut(s) 33, 318
BsiHKAI GWGCWC 1 cut(s) 93
BsiHKCI CYCGRG 4 cut(s) 86, 263, 368, 388
BsiSI CCGG 1 cut(s) 319
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 325
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 2 cut(s) 33, 318
BsoBI CYCGRG 4 cut(s) 86, 263, 368, 388
Bsp1286I GDGCHC 1 cut(s) 93
Bsp143I GATC 2 cut(s) 157, 297
BspANI GGCC 2 cut(s) 33, 318
BspPI GGATC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 338
BssECI CCNNGG 2 cut(s) 115, 369
BssMI GATC 2 cut(s) 157, 297
BssNI GRCGYC 1 cut(s) 291
BssSI CACGAG 2 cut(s) 186, 301
Bst2BI CACGAG 2 cut(s) 186, 301
Bst2UI CCWGG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 291
BstDEI CTNAG 1 cut(s) 254
BstKTI GATC 2 cut(s) 160, 300
BstMBI GATC 2 cut(s) 157, 297
BstNI CCWGG 1 cut(s) 117
BstNSI RCATGY 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 115
Bsu36I CCTNAGG 1 cut(s) 254
BsuRI GGCC 2 cut(s) 33, 318
Cfr13I GGNCC 3 cut(s) 31, 193, 250
Csp6I GTAC 3 cut(s) 55, 122, 173
CviAII CATG 2 cut(s) 23, 182
CviJI RGCY 6 cut(s) 33, 91, 230, 241, 262, 318
CviKI_1 RGCY 6 cut(s) 33, 91, 230, 241, 262, 318
CviQI GTAC 3 cut(s) 55, 122, 173
DdeI CTNAG 1 cut(s) 254
DpnI GATC 2 cut(s) 159, 299
DpnII GATC 2 cut(s) 157, 297
EaeI YGGCCR 1 cut(s) 316
Ecl136II GAGCTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 93
Eco47I GGWCC 2 cut(s) 193, 250
Eco53kI GAGCTC 1 cut(s) 91
Eco81I CCTNAGG 1 cut(s) 254
Eco88I CYCGRG 4 cut(s) 86, 263, 368, 388
EcoICRI GAGCTC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 250
EcoRII CCWGG 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 93
FaeI CATG 2 cut(s) 26, 185
FaiI YATR 7 cut(s) 24, 130, 177, 183, 212, 351, 353
FaqI GGGAC 1 cut(s) 69
FatI CATG 2 cut(s) 22, 181
FriOI GRGCYC 1 cut(s) 93
HaeIII GGCC 2 cut(s) 33, 318
HapII CCGG 1 cut(s) 319
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 2 cut(s) 26, 185
HindIII AAGCTT 2 cut(s) 228, 239
HinfI GANTC 1 cut(s) 202
HpaII CCGG 1 cut(s) 319
HphI GGTGA 2 cut(s) 257, 368
Hpy166II GTNNAC 2 cut(s) 57, 122
Hpy188III TCNNGA 5 cut(s) 86, 188, 295, 301, 368
Hpy8I GTNNAC 2 cut(s) 57, 122
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 4 cut(s) 105, 110, 160, 420
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4IV ACGT 1 cut(s) 291
HpyCH4V TGCA 1 cut(s) 77
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 1 cut(s) 291
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 2 cut(s) 26, 185
Kzo9I GATC 2 cut(s) 157, 297
LmnI GCTCC 1 cut(s) 96
LpnPI CCDG 7 cut(s) 87, 94, 102, 129, 332, 351, 380
MaeII ACGT 1 cut(s) 291
MaeIII GTNAC 2 cut(s) 383, 401
MalI GATC 2 cut(s) 159, 299
MboI GATC 2 cut(s) 157, 297
MboII GAAGA 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 93
MlyI GAGTC 1 cut(s) 211
MnlI CCTC 5 cut(s) 184, 241, 252, 364, 384
MslI CAYNNNNRTG 3 cut(s) 180, 186, 354
MspI CCGG 1 cut(s) 319
MspR9I CCNGG 1 cut(s) 117
MvaI CCWGG 1 cut(s) 117
NdeII GATC 2 cut(s) 157, 297
NlaIII CATG 2 cut(s) 26, 185
NmuCI GTSAC 1 cut(s) 383
NspI RCATGY 1 cut(s) 26
PaeR7I CTCGAG 1 cut(s) 388
PleI GAGTC 1 cut(s) 210
PpsI GAGTC 1 cut(s) 210
PpuMI RGGWCCY 1 cut(s) 250
Psp124BI GAGCTC 1 cut(s) 93
Psp5II RGGWCCY 1 cut(s) 250
Psp6I CCWGG 1 cut(s) 115
PspGI CCWGG 1 cut(s) 115
PspPI GGNCC 3 cut(s) 31, 193, 250
PspPPI RGGWCCY 1 cut(s) 250
PspXI VCTCGAGB 1 cut(s) 388
RsaI GTAC 3 cut(s) 56, 123, 174
RsaNI GTAC 3 cut(s) 55, 122, 173
RseI CAYNNNNRTG 3 cut(s) 180, 186, 354
SacI GAGCTC 1 cut(s) 93
Sau3AI GATC 2 cut(s) 157, 297
Sau96I GGNCC 3 cut(s) 31, 193, 250
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 1 cut(s) 117
SduI GDGCHC 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 388
SinI GGWCC 2 cut(s) 193, 250
SlaI CTCGAG 1 cut(s) 388
SmiMI CAYNNNNRTG 3 cut(s) 180, 186, 354
SmlI CTYRAG 1 cut(s) 388
SmoI CTYRAG 1 cut(s) 388
SstI GAGCTC 1 cut(s) 93
StyD4I CCNGG 1 cut(s) 115
TaaI ACNGT 1 cut(s) 54
TaiI ACGT 1 cut(s) 294
TaqI TCGA 2 cut(s) 160, 389
TatI WGTACW 1 cut(s) 172
TseFI GTSAC 1 cut(s) 383
Tsp45I GTSAC 1 cut(s) 383
TspGWI ACGGA 1 cut(s) 139
VpaK11BI GGWCC 2 cut(s) 193, 250
XceI RCATGY 1 cut(s) 26
XhoI CTCGAG 1 cut(s) 388
ZraI GACGTC 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.