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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 179, 522 |
| AccBSI | CCGCTC | 1 cut(s) | 500 |
| AciI | CCGC | 1 cut(s) | 498 |
| AcsI | RAATTY | 2 cut(s) | 124, 301 |
| AfaI | GTAC | 8 cut(s) | 85, 136, 164, 185, 207, 376, 658, 1039 |
| AflIII | ACRYGT | 2 cut(s) | 533, 573 |
| AgsI | TTSAA | 4 cut(s) | 287, 301, 406, 986 |
| AjnI | CCWGG | 1 cut(s) | 850 |
| AluBI | AGCT | 1 cut(s) | 673 |
| AluI | AGCT | 1 cut(s) | 673 |
| ApoI | RAATTY | 2 cut(s) | 124, 301 |
| AseI | ATTAAT | 3 cut(s) | 95, 201, 354 |
| AsuHPI | GGTGA | 1 cut(s) | 494 |
| BaeGI | GKGCMC | 1 cut(s) | 567 |
| BccI | CCATC | 1 cut(s) | 649 |
| BciT130I | CCWGG | 1 cut(s) | 852 |
| BciVI | GTATCC | 1 cut(s) | 222 |
| BfaI | CTAG | 1 cut(s) | 587 |
| BfuI | GTATCC | 1 cut(s) | 222 |
| Bme1390I | CCNGG | 1 cut(s) | 852 |
| BmrFI | CCNGG | 1 cut(s) | 852 |
| BmsI | GCATC | 1 cut(s) | 865 |
| BpmI | CTGGAG | 1 cut(s) | 873 |
| BpuEI | CTTGAG | 1 cut(s) | 920 |
| BsaBI | GATNNNNATC | 1 cut(s) | 393 |
| Bse8I | GATNNNNATC | 1 cut(s) | 393 |
| BseBI | CCWGG | 1 cut(s) | 852 |
| BseGI | GGATG | 1 cut(s) | 641 |
| BseJI | GATNNNNATC | 1 cut(s) | 393 |
| BseSI | GKGCMC | 1 cut(s) | 567 |
| Bsp1286I | GDGCHC | 1 cut(s) | 567 |
| Bsp1407I | TGTACA | 1 cut(s) | 374 |
| Bsp143I | GATC | 1 cut(s) | 394 |
| BspACI | CCGC | 1 cut(s) | 498 |
| BsrBI | CCGCTC | 1 cut(s) | 500 |
| BsrGI | TGTACA | 1 cut(s) | 374 |
| BssMI | GATC | 1 cut(s) | 394 |
| Bst2UI | CCWGG | 1 cut(s) | 852 |
| Bst4CI | ACNGT | 5 cut(s) | 319, 569, 667, 764, 794 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 451 |
| BstAUI | TGTACA | 1 cut(s) | 374 |
| BstDEI | CTNAG | 2 cut(s) | 506, 1019 |
| BstF5I | GGATG | 1 cut(s) | 641 |
| BstKTI | GATC | 1 cut(s) | 397 |
| BstMBI | GATC | 1 cut(s) | 394 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 451 |
| BstNI | CCWGG | 1 cut(s) | 852 |
| BstNSI | RCATGY | 2 cut(s) | 537, 577 |
| BstSCI | CCNGG | 1 cut(s) | 850 |
| BstSLI | GKGCMC | 1 cut(s) | 567 |
| BsuI | GTATCC | 1 cut(s) | 222 |
| BtsCI | GGATG | 1 cut(s) | 641 |
| BtsIMutI | CAGTG | 2 cut(s) | 456, 565 |
| CseI | GACGC | 1 cut(s) | 599 |
| Csp6I | GTAC | 8 cut(s) | 84, 135, 163, 184, 206, 375, 657, 1038 |
| CviAII | CATG | 6 cut(s) | 89, 534, 574, 623, 881, 1007 |
| CviJI | RGCY | 4 cut(s) | 23, 673, 751, 897 |
| CviKI_1 | RGCY | 4 cut(s) | 23, 673, 751, 897 |
| CviQI | GTAC | 8 cut(s) | 84, 135, 163, 184, 206, 375, 657, 1038 |
| DdeI | CTNAG | 2 cut(s) | 506, 1019 |
| DpnI | GATC | 1 cut(s) | 396 |
| DpnII | GATC | 1 cut(s) | 394 |
| DraI | TTTAAA | 2 cut(s) | 123, 846 |
| EcoRII | CCWGG | 1 cut(s) | 850 |
| FaeI | CATG | 6 cut(s) | 92, 537, 577, 626, 884, 1010 |
| FatI | CATG | 6 cut(s) | 88, 533, 573, 622, 880, 1006 |
| FokI | GGATG | 1 cut(s) | 628 |
| FspBI | CTAG | 1 cut(s) | 587 |
| GsuI | CTGGAG | 1 cut(s) | 873 |
| HgaI | GACGC | 1 cut(s) | 599 |
| Hin1II | CATG | 6 cut(s) | 92, 537, 577, 626, 884, 1010 |
| HincII | GTYRAC | 2 cut(s) | 680, 744 |
| HindII | GTYRAC | 2 cut(s) | 680, 744 |
| HpaI | GTTAAC | 1 cut(s) | 744 |
| HphI | GGTGA | 1 cut(s) | 494 |
| Hpy166II | GTNNAC | 3 cut(s) | 375, 680, 744 |
| Hpy188I | TCNGA | 1 cut(s) | 597 |
| Hpy188III | TCNNGA | 1 cut(s) | 587 |
| Hpy8I | GTNNAC | 3 cut(s) | 375, 680, 744 |
| HpyAV | CCTTC | 3 cut(s) | 465, 646, 831 |
| HpyCH4III | ACNGT | 5 cut(s) | 319, 569, 667, 764, 794 |
| HpyCH4IV | ACGT | 2 cut(s) | 676, 746 |
| HpyCH4V | TGCA | 2 cut(s) | 445, 949 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 451 |
| HpyF3I | CTNAG | 2 cut(s) | 506, 1019 |
| HpySE526I | ACGT | 2 cut(s) | 676, 746 |
| Hsp92II | CATG | 6 cut(s) | 92, 537, 577, 626, 884, 1010 |
| KspAI | GTTAAC | 1 cut(s) | 744 |
| Kzo9I | GATC | 1 cut(s) | 394 |
| LpnPI | CCDG | 3 cut(s) | 837, 864, 1063 |
| LweI | GCATC | 1 cut(s) | 865 |
| MaeI | CTAG | 1 cut(s) | 587 |
| MaeII | ACGT | 2 cut(s) | 676, 746 |
| MaeIII | GTNAC | 6 cut(s) | 261, 313, 529, 569, 625, 794 |
| MalI | GATC | 1 cut(s) | 396 |
| MbiI | CCGCTC | 1 cut(s) | 500 |
| MboI | GATC | 1 cut(s) | 394 |
| MboII | GAAGA | 5 cut(s) | 487, 685, 786, 789, 896 |
| MfeI | CAATTG | 1 cut(s) | 986 |
| MhlI | GDGCHC | 1 cut(s) | 567 |
| MluCI | AATT | 9 cut(s) | 78, 96, 124, 142, 210, 301, 479, 890, 986 |
| MmeI | TCCRAC | 1 cut(s) | 958 |
| MnlI | CCTC | 7 cut(s) | 177, 363, 597, 828, 848, 895, 946 |
| MspR9I | CCNGG | 1 cut(s) | 852 |
| MunI | CAATTG | 1 cut(s) | 986 |
| MvaI | CCWGG | 1 cut(s) | 852 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 451 |
| NdeII | GATC | 1 cut(s) | 394 |
| NlaIII | CATG | 6 cut(s) | 92, 537, 577, 626, 884, 1010 |
| NmuCI | GTSAC | 2 cut(s) | 529, 569 |
| NspI | RCATGY | 2 cut(s) | 537, 577 |
| PciI | ACATGT | 2 cut(s) | 533, 573 |
| PfoI | TCCNGGA | 1 cut(s) | 850 |
| PscI | ACATGT | 2 cut(s) | 533, 573 |
| PshBI | ATTAAT | 3 cut(s) | 95, 201, 354 |
| PsiI | TTATAA | 2 cut(s) | 179, 522 |
| Psp6I | CCWGG | 1 cut(s) | 850 |
| PspGI | CCWGG | 1 cut(s) | 850 |
| RsaI | GTAC | 8 cut(s) | 85, 136, 164, 185, 207, 376, 658, 1039 |
| RsaNI | GTAC | 8 cut(s) | 84, 135, 163, 184, 206, 375, 657, 1038 |
| Sau3AI | GATC | 1 cut(s) | 394 |
| ScrFI | CCNGG | 1 cut(s) | 852 |
| SduI | GDGCHC | 1 cut(s) | 567 |
| SetI | ASST | 9 cut(s) | 189, 374, 507, 675, 679, 749, 859, 957, 1082 |
| SfaNI | GCATC | 1 cut(s) | 865 |
| SmlI | CTYRAG | 1 cut(s) | 899 |
| SmoI | CTYRAG | 1 cut(s) | 899 |
| Sse9I | AATT | 9 cut(s) | 78, 96, 124, 142, 210, 301, 479, 890, 986 |
| SsiI | CCGC | 1 cut(s) | 498 |
| SspI | AATATT | 1 cut(s) | 61 |
| SspMI | CTAG | 1 cut(s) | 587 |
| StyD4I | CCNGG | 1 cut(s) | 850 |
| TaaI | ACNGT | 5 cut(s) | 319, 569, 667, 764, 794 |
| TaiI | ACGT | 2 cut(s) | 679, 749 |
| TaqI | TCGA | 1 cut(s) | 397 |
| TasI | AATT | 9 cut(s) | 78, 96, 124, 142, 210, 301, 479, 890, 986 |
| TatI | WGTACW | 3 cut(s) | 134, 374, 1037 |
| TscAI | CASTG | 2 cut(s) | 456, 572 |
| TseFI | GTSAC | 2 cut(s) | 529, 569 |
| Tsp45I | GTSAC | 2 cut(s) | 529, 569 |
| TspDTI | ATGAA | 3 cut(s) | 118, 897, 1073 |
| TspRI | CASTG | 2 cut(s) | 456, 572 |
| VspI | ATTAAT | 3 cut(s) | 95, 201, 354 |
| XapI | RAATTY | 2 cut(s) | 124, 301 |
| XbaI | TCTAGA | 1 cut(s) | 586 |
| XceI | RCATGY | 2 cut(s) | 537, 577 |
| XspI | CTAG | 1 cut(s) | 587 |