pycom16g24640

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0000513
Physical Location & Seq
Reverse (-)
26240783 .. 26242614
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g24640.3

Sequence Viewer

Length: 396 bp
ATGGTGGACCACACATTTCCCTCACTGTTTTCTCCCTTTCCCTGCACCTGTCCCGAGTTCCCTTTCCCATCCCATTTTCCATTCAACGTACGGACACACACCCAAACCCATCAAACCTCCACAGATCGATGGAACCAAGCACATATTCATGCTCGTGAGGCTCGTAGGAGTCCAACGATACCCATTTCAGTTCAAGCTTGTAGGAAGCTTGGTGAGGTCCCTAGGAGGCTCGGGGTGGTTCGTTTGAAGGTTTTGGACGTCGGGATCACGAGTTTCGAGGTTGGCCTGAGTTTGGGTGATTTTCCAATTGTTTCGCATATAGGTGATACCTATCCCGAGGACGAGCGTGGTCACTCGAGGCAGGGGGGCTACGACCCTTCCACATACCAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.85

Weight (kDa)

7.09

Isoelectric Point (pI)

64.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
AclWI GGATC 1 cut(s) 272
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 1 cut(s) 90
AfiI CCNNNNNNNGG 1 cut(s) 292
AgsI TTSAA 3 cut(s) 85, 194, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 129, 161
AluBI AGCT 2 cut(s) 197, 208
AluI AGCT 2 cut(s) 197, 208
AlwI GGATC 1 cut(s) 272
Ama87I CYCGRG 4 cut(s) 53, 230, 335, 355
AoxI GGCC 1 cut(s) 283
AspA2I CCTAGG 1 cut(s) 221
AspS9I GGNCC 2 cut(s) 7, 217
AsuHPI GGTGA 3 cut(s) 224, 308, 335
AvaI CYCGRG 4 cut(s) 53, 230, 335, 355
AvaII GGWCC 2 cut(s) 7, 217
AvrII CCTAGG 1 cut(s) 221
BauI CACGAG 2 cut(s) 153, 268
BccI CCATC 3 cut(s) 76, 117, 123
BfaI CTAG 2 cut(s) 222, 394
BlnI CCTAGG 1 cut(s) 221
Bme18I GGWCC 2 cut(s) 7, 217
BmeT110I CYCGRG 4 cut(s) 53, 230, 335, 355
BmgT120I GGNCC 2 cut(s) 7, 217
BmiI GGNNCC 2 cut(s) 134, 219
Bsa29I ATCGAT 1 cut(s) 127
BsaBI GATNNNNATC 1 cut(s) 330
BsaHI GRCGYC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 221, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 292
Bse1I ACTGG 1 cut(s) 388
Bse8I GATNNNNATC 1 cut(s) 330
BseCI ATCGAT 1 cut(s) 127
BseDI CCNNGG 2 cut(s) 221, 336
BseGI GGATG 1 cut(s) 68
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 1 cut(s) 292
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 1 cut(s) 388
BsgI GTGCAG 1 cut(s) 28
BshFI GGCC 1 cut(s) 285
BshVI ATCGAT 1 cut(s) 127
BsiHKCI CYCGRG 4 cut(s) 53, 230, 335, 355
BsiWI CGTACG 1 cut(s) 88
BslFI GGGAC 2 cut(s) 36, 203
BslI CCNNNNNNNGG 1 cut(s) 292
BsmFI GGGAC 2 cut(s) 36, 203
BsnI GGCC 1 cut(s) 285
BsoBI CYCGRG 4 cut(s) 53, 230, 335, 355
Bsp143I GATC 2 cut(s) 124, 264
BspANI GGCC 1 cut(s) 285
BspCNI CTCAG 1 cut(s) 279
BspDI ATCGAT 1 cut(s) 127
BspLI GGNNCC 2 cut(s) 134, 219
BspPI GGATC 1 cut(s) 272
BsrI ACTGG 1 cut(s) 388
BssECI CCNNGG 2 cut(s) 221, 336
BssMI GATC 2 cut(s) 124, 264
BssNI GRCGYC 1 cut(s) 258
BssSI CACGAG 2 cut(s) 153, 268
BssT1I CCWWGG 1 cut(s) 221
Bst2BI CACGAG 2 cut(s) 153, 268
Bst4CI ACNGT 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 258
BstDEI CTNAG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 68
BstKTI GATC 2 cut(s) 127, 267
BstMBI GATC 2 cut(s) 124, 264
BstMWI GCNNNNNNNGC 1 cut(s) 158
Bsu15I ATCGAT 1 cut(s) 127
BsuRI GGCC 1 cut(s) 285
BsuTUI ATCGAT 1 cut(s) 127
BtsCI GGATG 1 cut(s) 68
BtsIMutI CAGTG 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 7, 217
ClaI ATCGAT 1 cut(s) 127
Csp6I GTAC 1 cut(s) 89
CviAII CATG 1 cut(s) 149
CviJI RGCY 6 cut(s) 161, 197, 208, 229, 285, 369
CviKI_1 RGCY 6 cut(s) 161, 197, 208, 229, 285, 369
CviQI GTAC 1 cut(s) 89
DdeI CTNAG 1 cut(s) 287
DpnI GATC 2 cut(s) 126, 266
DpnII GATC 2 cut(s) 124, 264
Eco130I CCWWGG 1 cut(s) 221
Eco47I GGWCC 2 cut(s) 7, 217
Eco88I CYCGRG 4 cut(s) 53, 230, 335, 355
EcoO109I RGGNCCY 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
FaeI CATG 1 cut(s) 152
FaiI YATR 5 cut(s) 144, 150, 318, 320, 385
FaqI GGGAC 2 cut(s) 36, 203
FatI CATG 1 cut(s) 148
FokI GGATG 1 cut(s) 55
FspBI CTAG 2 cut(s) 222, 394
HaeIII GGCC 1 cut(s) 285
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 1 cut(s) 152
HindIII AAGCTT 2 cut(s) 195, 206
HinfI GANTC 1 cut(s) 169
HphI GGTGA 3 cut(s) 224, 308, 335
Hpy166II GTNNAC 1 cut(s) 7
Hpy188III TCNNGA 5 cut(s) 53, 155, 262, 268, 335
Hpy8I GTNNAC 1 cut(s) 7
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 2 cut(s) 241, 387
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 2 cut(s) 87, 258
HpyCH4V TGCA 1 cut(s) 45
HpyF10VI GCNNNNNNNGC 1 cut(s) 158
HpyF3I CTNAG 1 cut(s) 287
HpySE526I ACGT 2 cut(s) 87, 258
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 1 cut(s) 152
Kzo9I GATC 2 cut(s) 124, 264
LpnPI CCDG 4 cut(s) 55, 61, 299, 347
MaeI CTAG 2 cut(s) 222, 394
MaeII ACGT 2 cut(s) 87, 258
MaeIII GTNAC 1 cut(s) 350
MalI GATC 2 cut(s) 126, 266
MboI GATC 2 cut(s) 124, 264
MfeI CAATTG 1 cut(s) 306
MluCI AATT 1 cut(s) 306
MlyI GAGTC 1 cut(s) 178
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 8 cut(s) 31, 127, 151, 208, 219, 271, 331, 351
MslI CAYNNNNRTG 3 cut(s) 147, 153, 321
MunI CAATTG 1 cut(s) 306
MwoI GCNNNNNNNGC 1 cut(s) 158
NdeII GATC 2 cut(s) 124, 264
NlaIII CATG 1 cut(s) 152
NlaIV GGNNCC 2 cut(s) 134, 219
NmuCI GTSAC 1 cut(s) 350
PaeR7I CTCGAG 1 cut(s) 355
PcsI WCGNNNNNNNCGW 1 cut(s) 160
Pfl23II CGTACG 1 cut(s) 88
PleI GAGTC 1 cut(s) 177
PpsI GAGTC 1 cut(s) 177
PpuMI RGGWCCY 1 cut(s) 217
Psp5II RGGWCCY 1 cut(s) 217
PspLI CGTACG 1 cut(s) 88
PspN4I GGNNCC 2 cut(s) 134, 219
PspPI GGNCC 2 cut(s) 7, 217
PspPPI RGGWCCY 1 cut(s) 217
PspXI VCTCGAGB 1 cut(s) 355
RsaI GTAC 1 cut(s) 90
RsaNI GTAC 1 cut(s) 89
RseI CAYNNNNRTG 3 cut(s) 147, 153, 321
Sau3AI GATC 2 cut(s) 124, 264
Sau96I GGNCC 2 cut(s) 7, 217
SchI GAGTC 1 cut(s) 178
Sfr274I CTCGAG 1 cut(s) 355
SinI GGWCC 2 cut(s) 7, 217
SlaI CTCGAG 1 cut(s) 355
SmiMI CAYNNNNRTG 3 cut(s) 147, 153, 321
SmlI CTYRAG 1 cut(s) 355
SmoI CTYRAG 1 cut(s) 355
Sse9I AATT 1 cut(s) 306
SspMI CTAG 2 cut(s) 222, 394
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 2 cut(s) 90, 261
TaqI TCGA 3 cut(s) 127, 276, 356
TasI AATT 1 cut(s) 306
TscAI CASTG 1 cut(s) 30
TseFI GTSAC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 350
TspDTI ATGAA 1 cut(s) 137
TspGWI ACGGA 1 cut(s) 106
TspRI CASTG 1 cut(s) 30
VpaK11BI GGWCC 2 cut(s) 7, 217
XhoI CTCGAG 1 cut(s) 355
XmaJI CCTAGG 1 cut(s) 221
XspI CTAG 2 cut(s) 222, 394
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.