pycom05g24030

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000513
Physical Location & Seq
Forward (+)
25612770 .. 25613973
1204 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g24030.2

Sequence Viewer

Length: 930 bp
ATGGCCGAATTATGTACTGTTCATGCAACCCTTAAACTAGCTTGTTTTTGCAATTTTGAAGCTTGTAGTTGTGTTTGTGAGGTCCCAAGGAGCCTCGGAGTGGTTCGATGGGTGAAATTGGACGTCGGGAAGGCTAGGTTCGAAGTTGGCCGAACTTGGATCGTTTCTAACGTGATTCTACTAAAACGAAGGAGAAAACAAAGTTTGAAAATTTTCCAGTTTTCCGGCGCCGTCGCCGGCGCCAGAGTCTCGCCGGAGAAGGTGACGGAATATTCCGTCAAATCTGACGGAATATTCCTAACGGCAGTGACGGCGTCAGTTAAGTTTAACGGAATATTCCGTCAGTTTAACGGAATATTCCTGACGGCGTCAGTTGACGCCGTCAGTGTGCATGGACGGCGCGTGGGGGCGCGTGCGTGGTCCAAAAATTATTCTAAAAATATGGGTGTGATCCTGAGGTTGTGTCGATCACGTTGGTATATTCGTTTTAACGTTTTCGTAGCTTTTTCGCGTATAGGTGATTCGTTTTCCGAGGACGAGCGTTCACACTCGAGGCAAGGGGGCTACGACCCTTCTAATTATCATAATTCCCAGAAATTAAATAGAAAAGGTTATTTGTTTTATGCCATGCATCATATGAATGTTGTTTACGCATCATCGCATGTATTAGTAGTGACATACATATATATATATGTGTATTGGTGCTGTGGACGCACAGGTAAGTGCCAGAGAGCTCATAATCTGCACCCCGGTGTTAGTGCTCCCGCCCAGGGCCAGGGCATAGTCCTTCACGTGATGTTCACCCGCACCACACGCTCGCCTTGGATGCAAGTTAGGTGCAAGCCTTGTCGTGCAGACCACATTAGGTGGTTCCGACTCGTAGGTGACCCGCGATTATTCGCACAGTCTTCATGTGATCGTAGCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

310

Amino Acids

35.18

Weight (kDa)

10.07

Isoelectric Point (pI)

49.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccB1I GGYRCC 2 cut(s) 227, 239
AccII CGCG 4 cut(s) 402, 412, 511, 892
AciI CCGC 3 cut(s) 765, 805, 890
AclI AACGTT 1 cut(s) 492
AclWI GGATC 2 cut(s) 167, 445
AcoI YGGCCR 2 cut(s) 3, 148
AcsI RAATTY 1 cut(s) 210
AcvI CACGTG 1 cut(s) 793
AcyI GRCGYC 6 cut(s) 123, 228, 240, 314, 368, 378
AfaI GTAC 1 cut(s) 16
AfiI CCNNNNNNNGG 3 cut(s) 100, 770, 775
AgsI TTSAA 2 cut(s) 59, 208
AjnI CCWGG 2 cut(s) 768, 774
AleI CACNNNNGTG 1 cut(s) 750
AluBI AGCT 4 cut(s) 41, 62, 503, 734
AluI AGCT 4 cut(s) 41, 62, 503, 734
Alw21I GWGCWC 2 cut(s) 736, 763
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 2 cut(s) 167, 445
Ama87I CYCGRG 1 cut(s) 550
AoxI GGCC 3 cut(s) 3, 148, 772
ApoI RAATTY 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 212
AspLEI GCGC 4 cut(s) 230, 242, 402, 412
AspS9I GGNCC 3 cut(s) 82, 420, 772
AsuC2I CCSGG 1 cut(s) 750
AsuHPI GGTGA 5 cut(s) 124, 274, 530, 793, 896
AsuII TTCGAA 1 cut(s) 141
AvaI CYCGRG 1 cut(s) 550
AvaII GGWCC 2 cut(s) 82, 420
AxyI CCTNAGG 1 cut(s) 455
BanI GGYRCC 2 cut(s) 227, 239
BanII GRGCYC 1 cut(s) 736
BbrPI CACGTG 1 cut(s) 793
BbsI GAAGAC 1 cut(s) 900
Bbv12I GWGCWC 2 cut(s) 736, 763
BccI CCATC 1 cut(s) 102
BceAI ACGGC 6 cut(s) 215, 318, 327, 365, 381, 413
BciT130I CCWGG 2 cut(s) 770, 776
BcnI CCSGG 1 cut(s) 750
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 2 cut(s) 38, 135
BfoI RGCGCY 2 cut(s) 231, 243
Bme1390I CCNGG 3 cut(s) 750, 770, 776
Bme18I GGWCC 2 cut(s) 82, 420
BmeT110I CYCGRG 1 cut(s) 550
BmgT120I GGNCC 3 cut(s) 82, 420, 772
BmiI GGNNCC 5 cut(s) 84, 92, 229, 241, 872
BmrFI CCNGG 3 cut(s) 750, 770, 776
BmsI GCATC 3 cut(s) 640, 662, 816
BpiI GAAGAC 1 cut(s) 900
Bpu14I TTCGAA 1 cut(s) 141
BpuMI CCSGG 1 cut(s) 750
BsaAI YACGTR 1 cut(s) 793
BsaHI GRCGYC 6 cut(s) 123, 228, 240, 314, 368, 378
BsaJI CCNNGG 8 cut(s) 86, 94, 531, 748, 768, 769, 775, 821
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 770, 775
Bse118I RCCGGY 1 cut(s) 236
Bse1I ACTGG 1 cut(s) 217
Bse21I CCTNAGG 1 cut(s) 455
BseBI CCWGG 2 cut(s) 770, 776
BseDI CCNNGG 8 cut(s) 86, 94, 531, 748, 768, 769, 775, 821
BseGI GGATG 1 cut(s) 831
BseLI CCNNNNNNNGG 3 cut(s) 100, 770, 775
BseMII CTCAG 1 cut(s) 446
BseNI ACTGG 1 cut(s) 217
BsgI GTGCAG 2 cut(s) 728, 873
Bsh1236I CGCG 4 cut(s) 402, 412, 511, 892
BshFI GGCC 3 cut(s) 5, 150, 774
BshNI GGYRCC 2 cut(s) 227, 239
BsiHKAI GWGCWC 2 cut(s) 736, 763
BsiHKCI CYCGRG 1 cut(s) 550
BsiSI CCGG 4 cut(s) 225, 237, 254, 750
BslFI GGGAC 1 cut(s) 68
BslI CCNNNNNNNGG 3 cut(s) 100, 770, 775
BsmAI GTCTC 1 cut(s) 253
BsmFI GGGAC 1 cut(s) 68
BsnI GGCC 3 cut(s) 5, 150, 774
BsoBI CYCGRG 1 cut(s) 550
Bsp119I TTCGAA 1 cut(s) 141
Bsp1286I GDGCHC 2 cut(s) 736, 763
Bsp143I GATC 4 cut(s) 159, 450, 467, 916
BspACI CCGC 3 cut(s) 765, 805, 890
BspANI GGCC 3 cut(s) 5, 150, 774
BspCNI CTCAG 1 cut(s) 447
BspFNI CGCG 4 cut(s) 402, 412, 511, 892
BspLI GGNNCC 5 cut(s) 84, 92, 229, 241, 872
BspPI GGATC 2 cut(s) 167, 445
BspT104I TTCGAA 1 cut(s) 141
BspT107I GGYRCC 2 cut(s) 227, 239
BsrFI RCCGGY 1 cut(s) 236
BsrI ACTGG 1 cut(s) 217
BssAI RCCGGY 1 cut(s) 236
BssECI CCNNGG 8 cut(s) 86, 94, 531, 748, 768, 769, 775, 821
BssMI GATC 4 cut(s) 159, 450, 467, 916
BssNI GRCGYC 6 cut(s) 123, 228, 240, 314, 368, 378
BssT1I CCWWGG 2 cut(s) 86, 821
Bst2UI CCWGG 2 cut(s) 770, 776
Bst4CI ACNGT 2 cut(s) 19, 906
BstACI GRCGYC 6 cut(s) 123, 228, 240, 314, 368, 378
BstBAI YACGTR 1 cut(s) 793
BstBI TTCGAA 1 cut(s) 141
BstC8I GCNNGC 4 cut(s) 238, 414, 818, 842
BstDEI CTNAG 1 cut(s) 455
BstEII GGTNACC 1 cut(s) 884
BstF5I GGATG 1 cut(s) 831
BstFNI CGCG 4 cut(s) 402, 412, 511, 892
BstH2I RGCGCY 2 cut(s) 231, 243
BstHHI GCGC 4 cut(s) 230, 242, 402, 412
BstKTI GATC 4 cut(s) 162, 453, 470, 919
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 4 cut(s) 159, 450, 467, 916
BstMWI GCNNNNNNNGC 5 cut(s) 311, 397, 711, 813, 826
BstNI CCWGG 2 cut(s) 770, 776
BstNSI RCATGY 1 cut(s) 665
BstPI GGTNACC 1 cut(s) 884
BstSCI CCNGG 3 cut(s) 748, 768, 774
BstUI CGCG 4 cut(s) 402, 412, 511, 892
BstV2I GAAGAC 1 cut(s) 900
Bsu36I CCTNAGG 1 cut(s) 455
BsuRI GGCC 3 cut(s) 5, 150, 774
BtgZI GCGATG 1 cut(s) 642
BtsCI GGATG 1 cut(s) 831
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 312, 391
Cac8I GCNNGC 4 cut(s) 238, 414, 818, 842
CfoI GCGC 4 cut(s) 230, 242, 402, 412
Cfr10I RCCGGY 1 cut(s) 236
Cfr13I GGNCC 3 cut(s) 82, 420, 772
CseI GACGC 4 cut(s) 303, 357, 386, 720
Csp6I GTAC 1 cut(s) 15
CviAII CATG 5 cut(s) 23, 392, 628, 662, 912
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 1 cut(s) 455
DinI GGCGCC 2 cut(s) 229, 241
DpnI GATC 4 cut(s) 161, 452, 469, 918
DpnII GATC 4 cut(s) 159, 450, 467, 916
EaeI YGGCCR 2 cut(s) 3, 148
Ecl136II GAGCTC 1 cut(s) 734
Eco130I CCWWGG 2 cut(s) 86, 821
Eco24I GRGCYC 1 cut(s) 736
Eco47I GGWCC 2 cut(s) 82, 420
Eco53kI GAGCTC 1 cut(s) 734
Eco72I CACGTG 1 cut(s) 793
Eco81I CCTNAGG 1 cut(s) 455
Eco88I CYCGRG 1 cut(s) 550
Eco91I GGTNACC 1 cut(s) 884
EcoICRI GAGCTC 1 cut(s) 734
EcoO109I RGGNCCY 1 cut(s) 82
EcoO65I GGTNACC 1 cut(s) 884
EcoRII CCWGG 2 cut(s) 768, 774
EcoT14I CCWWGG 2 cut(s) 86, 821
EcoT22I ATGCAT 1 cut(s) 633
EcoT38I GRGCYC 1 cut(s) 736
EgeI GGCGCC 2 cut(s) 229, 241
EheI GGCGCC 2 cut(s) 229, 241
ErhI CCWWGG 2 cut(s) 86, 821
FaeI CATG 5 cut(s) 26, 395, 631, 665, 915
FaqI GGGAC 1 cut(s) 68
FatI CATG 5 cut(s) 22, 391, 627, 661, 911
FauI CCCGC 3 cut(s) 772, 812, 897
FauNDI CATATG 1 cut(s) 636
FokI GGATG 1 cut(s) 838
FriOI GRGCYC 1 cut(s) 736
FspBI CTAG 2 cut(s) 38, 135
GlaI GCGC 4 cut(s) 229, 241, 401, 411
HaeII RGCGCY 2 cut(s) 231, 243
HaeIII GGCC 3 cut(s) 5, 150, 774
HapII CCGG 4 cut(s) 225, 237, 254, 750
HgaI GACGC 4 cut(s) 303, 357, 386, 720
HhaI GCGC 4 cut(s) 230, 242, 402, 412
Hin1I GRCGYC 6 cut(s) 123, 228, 240, 314, 368, 378
Hin1II CATG 5 cut(s) 26, 395, 631, 665, 915
Hin6I GCGC 4 cut(s) 228, 240, 400, 410
HinP1I GCGC 4 cut(s) 228, 240, 400, 410
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 60
HinfI GANTC 4 cut(s) 175, 246, 521, 876
HpaII CCGG 4 cut(s) 225, 237, 254, 750
HphI GGTGA 5 cut(s) 124, 274, 530, 793, 896
Hpy166II GTNNAC 5 cut(s) 376, 545, 649, 710, 801
Hpy188I TCNGA 4 cut(s) 98, 286, 532, 875
Hpy188III TCNNGA 3 cut(s) 127, 361, 454
Hpy8I GTNNAC 5 cut(s) 376, 545, 649, 710, 801
Hpy99I CGWCG 2 cut(s) 128, 236
HpyAV CCTTC 5 cut(s) 124, 183, 253, 582, 797
HpyCH4III ACNGT 2 cut(s) 19, 906
HpyCH4IV ACGT 5 cut(s) 123, 171, 472, 492, 792
HpyCH4V TGCA 8 cut(s) 26, 51, 391, 631, 745, 829, 840, 854
HpyF10VI GCNNNNNNNGC 5 cut(s) 311, 397, 711, 813, 826
HpyF3I CTNAG 1 cut(s) 455
HpySE526I ACGT 5 cut(s) 123, 171, 472, 492, 792
Hsp92I GRCGYC 6 cut(s) 123, 228, 240, 314, 368, 378
Hsp92II CATG 5 cut(s) 26, 395, 631, 665, 915
HspAI GCGC 4 cut(s) 228, 240, 400, 410
KasI GGCGCC 2 cut(s) 227, 239
KroI GCCGGC 1 cut(s) 236
KroNI GCCGGC 1 cut(s) 238
Kzo9I GATC 4 cut(s) 159, 450, 467, 916
LmnI GCTCC 2 cut(s) 90, 766
LweI GCATC 3 cut(s) 640, 662, 816
MaeI CTAG 2 cut(s) 38, 135
MaeII ACGT 5 cut(s) 123, 171, 472, 492, 792
MaeIII GTNAC 4 cut(s) 262, 307, 673, 884
MalI GATC 4 cut(s) 161, 452, 469, 918
MboI GATC 4 cut(s) 159, 450, 467, 916
MboII GAAGA 1 cut(s) 900
MhlI GDGCHC 2 cut(s) 736, 763
MluCI AATT 8 cut(s) 8, 52, 116, 210, 427, 577, 586, 596
Mly113I GGCGCC 2 cut(s) 228, 240
MlyI GAGTC 2 cut(s) 255, 870
MmeI TCCRAC 1 cut(s) 898
MnlI CCTC 5 cut(s) 73, 104, 450, 526, 546
Mph1103I ATGCAT 1 cut(s) 633
MreI CGCCGGCG 1 cut(s) 236
MroNI GCCGGC 1 cut(s) 236
MroXI GAANNNNTTC 1 cut(s) 212
MseI TTAA 6 cut(s) 33, 321, 327, 348, 489, 599
MslI CAYNNNNRTG 2 cut(s) 639, 750
MspI CCGG 4 cut(s) 225, 237, 254, 750
MspR9I CCNGG 3 cut(s) 750, 770, 776
MvaI CCWGG 2 cut(s) 770, 776
MvnI CGCG 4 cut(s) 402, 412, 511, 892
MwoI GCNNNNNNNGC 5 cut(s) 311, 397, 711, 813, 826
NaeI GCCGGC 1 cut(s) 238
NarI GGCGCC 2 cut(s) 228, 240
NciI CCSGG 1 cut(s) 750
NdeI CATATG 1 cut(s) 636
NdeII GATC 4 cut(s) 159, 450, 467, 916
NgoMIV GCCGGC 1 cut(s) 236
NlaIII CATG 5 cut(s) 26, 395, 631, 665, 915
NlaIV GGNNCC 5 cut(s) 84, 92, 229, 241, 872
NmuCI GTSAC 4 cut(s) 262, 307, 673, 884
NsiI ATGCAT 1 cut(s) 633
NspI RCATGY 1 cut(s) 665
NspV TTCGAA 1 cut(s) 141
OliI CACNNNNGTG 1 cut(s) 750
PaeR7I CTCGAG 1 cut(s) 550
PasI CCCWGGG 1 cut(s) 769
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PdiI GCCGGC 1 cut(s) 238
PdmI GAANNNNTTC 1 cut(s) 212
PfeI GAWTC 2 cut(s) 175, 521
PflFI GACNNNGTC 3 cut(s) 313, 367, 380
PleI GAGTC 2 cut(s) 254, 870
PluTI GGCGCC 2 cut(s) 231, 243
PmaCI CACGTG 1 cut(s) 793
PmlI CACGTG 1 cut(s) 793
PpsI GAGTC 2 cut(s) 254, 870
Ppu21I YACGTR 1 cut(s) 793
PpuMI RGGWCCY 1 cut(s) 82
Psp124BI GAGCTC 1 cut(s) 736
Psp1406I AACGTT 1 cut(s) 492
Psp5II RGGWCCY 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 768, 774
PspCI CACGTG 1 cut(s) 793
PspEI GGTNACC 1 cut(s) 884
PspGI CCWGG 2 cut(s) 768, 774
PspN4I GGNNCC 5 cut(s) 84, 92, 229, 241, 872
PspPI GGNCC 3 cut(s) 82, 420, 772
PspPPI RGGWCCY 1 cut(s) 82
PspXI VCTCGAGB 1 cut(s) 550
PsyI GACNNNGTC 3 cut(s) 313, 367, 380
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
RseI CAYNNNNRTG 2 cut(s) 639, 750
SacI GAGCTC 1 cut(s) 736
SaqAI TTAA 6 cut(s) 33, 321, 327, 348, 489, 599
Sau3AI GATC 4 cut(s) 159, 450, 467, 916
Sau96I GGNCC 3 cut(s) 82, 420, 772
SchI GAGTC 2 cut(s) 255, 870
ScrFI CCNGG 3 cut(s) 750, 770, 776
SduI GDGCHC 2 cut(s) 736, 763
SfaNI GCATC 3 cut(s) 640, 662, 816
SfoI GGCGCC 2 cut(s) 229, 241
Sfr274I CTCGAG 1 cut(s) 550
SfuI TTCGAA 1 cut(s) 141
SgrAI CRCCGGYG 1 cut(s) 236
SinI GGWCC 2 cut(s) 82, 420
SlaI CTCGAG 1 cut(s) 550
SmiMI CAYNNNNRTG 2 cut(s) 639, 750
SmlI CTYRAG 1 cut(s) 550
SmoI CTYRAG 1 cut(s) 550
Sse9I AATT 8 cut(s) 8, 52, 116, 210, 427, 577, 586, 596
SsiI CCGC 3 cut(s) 765, 805, 890
SspDI GGCGCC 2 cut(s) 227, 239
SspI AATATT 4 cut(s) 272, 294, 336, 357
SspMI CTAG 2 cut(s) 38, 135
SstI GAGCTC 1 cut(s) 736
StyD4I CCNGG 3 cut(s) 748, 768, 774
StyI CCWWGG 2 cut(s) 86, 821
TaaI ACNGT 2 cut(s) 19, 906
TaiI ACGT 5 cut(s) 126, 174, 475, 495, 795
TaqI TCGA 4 cut(s) 106, 141, 466, 551
TasI AATT 8 cut(s) 8, 52, 116, 210, 427, 577, 586, 596
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 2 cut(s) 175, 521
Tru1I TTAA 6 cut(s) 33, 321, 327, 348, 489, 599
Tru9I TTAA 6 cut(s) 33, 321, 327, 348, 489, 599
TscAI CASTG 2 cut(s) 312, 391
TseFI GTSAC 4 cut(s) 262, 307, 673, 884
Tsp45I GTSAC 4 cut(s) 262, 307, 673, 884
TspDTI ATGAA 3 cut(s) 11, 653, 900
TspGWI ACGGA 6 cut(s) 265, 281, 303, 329, 345, 366
TspRI CASTG 2 cut(s) 312, 391
Tth111I GACNNNGTC 3 cut(s) 313, 367, 380
VpaK11BI GGWCC 2 cut(s) 82, 420
XapI RAATTY 1 cut(s) 210
XceI RCATGY 1 cut(s) 665
XhoI CTCGAG 1 cut(s) 550
XmnI GAANNNNTTC 1 cut(s) 212
XspI CTAG 2 cut(s) 38, 135
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.