pycom13g26300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000513
Physical Location & Seq
Forward (+)
22991697 .. 22992433
737 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g26300.3

Sequence Viewer

Length: 474 bp
ATGTGTTGGTGGTTAAATGTTGGTTATAAATCAATGTGTGAAGAATCAAGAAATTTAACTAAGGAAACAAATAGTTTGAGAGTAGGGCATTACAGCTTATGCATTTTATGCCATATTATATATATGTATATAATTGAAAATGCTATGACATGGTTGATTATTAGAATGCATCATGGTATATCCATATATATATATATATATATATATATTACCTTTCAGGACTACATTTATGTGTTAGGCAACTCTGACACTGTCGTATTGGTTCTCATAAACCTACACCCCGGTGTTAGTGCTCCCGCATGTGGCCAGGGAACAATCCTTCACGTGATGTTCACCTCCTGCACCTTATGCTCACCTTGGACTCAAGGTAAGTGCACGGGCCTGTTTCTTCATGTGATCGTAACACTTGAGCATACTTATTTACACCCATTCCTGTCGTATAGACCTCTAGAACTGGTTCCGACTTGTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

18.1

Weight (kDa)

6.73

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 27
AciI CCGC 1 cut(s) 297
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 1 cut(s) 52
AcvI CACGTG 1 cut(s) 325
AfiI CCNNNNNNNGG 1 cut(s) 302
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 1 cut(s) 306
AleI CACNNNNGTG 1 cut(s) 282
AluBI AGCT 1 cut(s) 96
AluI AGCT 1 cut(s) 96
Alw21I GWGCWC 2 cut(s) 295, 377
Alw44I GTGCAC 1 cut(s) 373
AoxI GGCC 2 cut(s) 304, 379
ApaLI GTGCAC 1 cut(s) 373
ApoI RAATTY 1 cut(s) 52
Asp700I GAANNNNTTC 1 cut(s) 456
AspS9I GGNCC 1 cut(s) 379
AsuC2I CCSGG 1 cut(s) 282
AsuHPI GGTGA 2 cut(s) 325, 345
BaeGI GKGCMC 1 cut(s) 377
BalI TGGCCA 1 cut(s) 306
BbrPI CACGTG 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 295, 377
BciT130I CCWGG 1 cut(s) 308
BcnI CCSGG 1 cut(s) 282
BfaI CTAG 1 cut(s) 449
Bme1390I CCNGG 2 cut(s) 282, 308
BmgT120I GGNCC 1 cut(s) 379
BmiI GGNNCC 1 cut(s) 459
BmrFI CCNGG 2 cut(s) 282, 308
BmsI GCATC 1 cut(s) 178
BpuEI CTTGAG 2 cut(s) 348, 428
BpuMI CCSGG 1 cut(s) 282
BsaAI YACGTR 1 cut(s) 325
BsaJI CCNNGG 3 cut(s) 280, 307, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 308
BseDI CCNNGG 3 cut(s) 280, 307, 356
BseLI CCNNNNNNNGG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 459
BseSI GKGCMC 1 cut(s) 377
BsgI GTGCAG 1 cut(s) 325
BshFI GGCC 2 cut(s) 306, 381
BsiHKAI GWGCWC 2 cut(s) 295, 377
BsiSI CCGG 1 cut(s) 282
BslI CCNNNNNNNGG 1 cut(s) 302
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 2 cut(s) 306, 381
Bsp1286I GDGCHC 2 cut(s) 295, 377
Bsp143I GATC 1 cut(s) 396
BspACI CCGC 1 cut(s) 297
BspANI GGCC 2 cut(s) 306, 381
BspLI GGNNCC 1 cut(s) 459
BsrI ACTGG 1 cut(s) 459
BssECI CCNNGG 3 cut(s) 280, 307, 356
BssMI GATC 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 1 cut(s) 253
BstAPI GCANNNNNTGC 2 cut(s) 108, 348
BstBAI YACGTR 1 cut(s) 325
BstDEI CTNAG 1 cut(s) 60
BstKTI GATC 1 cut(s) 399
BstMBI GATC 1 cut(s) 396
BstMWI GCNNNNNNNGC 2 cut(s) 108, 348
BstNI CCWGG 1 cut(s) 308
BstNSI RCATGY 1 cut(s) 303
BstSCI CCNGG 2 cut(s) 280, 306
BstSLI GKGCMC 1 cut(s) 377
BsuRI GGCC 2 cut(s) 306, 381
BtsIMutI CAGTG 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 379
CviAII CATG 4 cut(s) 150, 173, 300, 392
CviJI RGCY 3 cut(s) 96, 306, 381
CviKI_1 RGCY 3 cut(s) 96, 306, 381
DdeI CTNAG 1 cut(s) 60
DpnI GATC 1 cut(s) 398
DpnII GATC 1 cut(s) 396
EaeI YGGCCR 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 356
Eco72I CACGTG 1 cut(s) 325
EcoRII CCWGG 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 356
EcoT22I ATGCAT 2 cut(s) 104, 171
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 4 cut(s) 153, 176, 303, 395
FatI CATG 4 cut(s) 149, 172, 299, 391
FauI CCCGC 1 cut(s) 304
FspBI CTAG 1 cut(s) 449
HaeIII GGCC 2 cut(s) 306, 381
HapII CCGG 1 cut(s) 282
Hin1II CATG 4 cut(s) 153, 176, 303, 395
HinfI GANTC 2 cut(s) 44, 361
HpaII CCGG 1 cut(s) 282
HphI GGTGA 2 cut(s) 325, 345
Hpy166II GTNNAC 2 cut(s) 333, 375
Hpy188I TCNGA 2 cut(s) 247, 462
Hpy188III TCNNGA 3 cut(s) 48, 218, 449
Hpy8I GTNNAC 2 cut(s) 333, 375
HpyAV CCTTC 1 cut(s) 329
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 4 cut(s) 102, 169, 342, 375
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 348
HpyF3I CTNAG 1 cut(s) 60
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 4 cut(s) 153, 176, 303, 395
Kzo9I GATC 1 cut(s) 396
LmnI GCTCC 1 cut(s) 298
LpnPI CCDG 8 cut(s) 203, 293, 295, 320, 352, 395, 440, 446
LweI GCATC 1 cut(s) 178
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 1 cut(s) 400
MalI GATC 1 cut(s) 398
MboI GATC 1 cut(s) 396
MboII GAAGA 2 cut(s) 53, 380
MhlI GDGCHC 2 cut(s) 295, 377
MlsI TGGCCA 1 cut(s) 306
MluCI AATT 2 cut(s) 52, 132
MluNI TGGCCA 1 cut(s) 306
MlyI GAGTC 1 cut(s) 355
MnlI CCTC 2 cut(s) 346, 456
Mox20I TGGCCA 1 cut(s) 306
Mph1103I ATGCAT 2 cut(s) 104, 171
MroXI GAANNNNTTC 1 cut(s) 456
MscI TGGCCA 1 cut(s) 306
MseI TTAA 2 cut(s) 14, 56
MslI CAYNNNNRTG 2 cut(s) 230, 282
Msp20I TGGCCA 1 cut(s) 306
MspI CCGG 1 cut(s) 282
MspR9I CCNGG 2 cut(s) 282, 308
Mva1269I GAATGC 1 cut(s) 171
MvaI CCWGG 1 cut(s) 308
MwoI GCNNNNNNNGC 2 cut(s) 108, 348
NciI CCSGG 1 cut(s) 282
NdeII GATC 1 cut(s) 396
NlaIII CATG 4 cut(s) 153, 176, 303, 395
NlaIV GGNNCC 1 cut(s) 459
NsiI ATGCAT 2 cut(s) 104, 171
NspI RCATGY 1 cut(s) 303
OliI CACNNNNGTG 1 cut(s) 282
PctI GAATGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 456
PfeI GAWTC 1 cut(s) 44
PflFI GACNNNGTC 1 cut(s) 251
PleI GAGTC 1 cut(s) 355
PmaCI CACGTG 1 cut(s) 325
PmlI CACGTG 1 cut(s) 325
PpsI GAGTC 1 cut(s) 355
Ppu21I YACGTR 1 cut(s) 325
PsiI TTATAA 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 306
PspCI CACGTG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 1 cut(s) 459
PspPI GGNCC 1 cut(s) 379
PsyI GACNNNGTC 1 cut(s) 251
RseI CAYNNNNRTG 2 cut(s) 230, 282
SaqAI TTAA 2 cut(s) 14, 56
Sau3AI GATC 1 cut(s) 396
Sau96I GGNCC 1 cut(s) 379
SchI GAGTC 1 cut(s) 355
ScrFI CCNGG 2 cut(s) 282, 308
SduI GDGCHC 2 cut(s) 295, 377
SetI ASST 9 cut(s) 98, 215, 276, 327, 338, 347, 358, 370, 448
SfaNI GCATC 1 cut(s) 178
SmiMI CAYNNNNRTG 2 cut(s) 230, 282
SmlI CTYRAG 2 cut(s) 363, 407
SmoI CTYRAG 2 cut(s) 363, 407
Sse9I AATT 2 cut(s) 52, 132
SsiI CCGC 1 cut(s) 297
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 2 cut(s) 280, 306
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 1 cut(s) 253
TaiI ACGT 1 cut(s) 327
TasI AATT 2 cut(s) 52, 132
TfiI GAWTC 1 cut(s) 44
Tru1I TTAA 2 cut(s) 14, 56
Tru9I TTAA 2 cut(s) 14, 56
TscAI CASTG 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 380
TspRI CASTG 1 cut(s) 256
Tth111I GACNNNGTC 1 cut(s) 251
VneI GTGCAC 1 cut(s) 373
XapI RAATTY 1 cut(s) 52
XbaI TCTAGA 1 cut(s) 448
XceI RCATGY 1 cut(s) 303
XmnI GAANNNNTTC 1 cut(s) 456
XspI CTAG 1 cut(s) 449
Zsp2I ATGCAT 2 cut(s) 104, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.