pycom14g12840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000513
Physical Location & Seq
Reverse (-)
15892377 .. 15893389
1013 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g12840.1

Sequence Viewer

Length: 465 bp
ATGGTTTTGCCCCTAGTGATTTTGTTTCGTTGTGTGGTCATTTTGGGGATTTGGTTGGCTGCATTTGGACCACACACACACCCCCACACTTTCAGTCTCATCCTCTGCCTGTCCCGAGTCTCTCTCTCTTCCTTCTTCTTCATTTGTGTACGGACAAGAACCAATTCACTCGAAAACGTGGTAGATCGAGGAAACAAATTACATATTTGTGCTCGTGAGGTTCTTAGGAGTTCAACCATACCCATTTCAGTTTGTAGGAAGCTTGGTGAGGTCCTTAGGAAGCTCGGGGTGGTTCGTTTGAAGGTTTTGGACGTTGGGATTGTGAGTTGCAGGTTTGGCCGGATTTGGTGGAGTTTTTCCGGCGAGTTTCCGTGGGTTTTAGTACTTGAAAGTGGTGAGACCTATCCCGAGGACGAGCGCAGTCACTCGAGGCAAGGGGGTTACGACCCTTCTACATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

155

Amino Acids

17.49

Weight (kDa)

9.27

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 321
AcoI YGGCCR 1 cut(s) 337
AfaI GTAC 2 cut(s) 150, 384
AgsI TTSAA 3 cut(s) 234, 301, 389
AluBI AGCT 2 cut(s) 262, 283
AluI AGCT 2 cut(s) 262, 283
Alw21I GWGCWC 1 cut(s) 214
Alw26I GTCTC 3 cut(s) 101, 124, 392
Ama87I CYCGRG 4 cut(s) 114, 284, 407, 427
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 1 cut(s) 59
Asp700I GAANNNNTTC 1 cut(s) 163
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 2 cut(s) 68, 271
AsuHPI GGTGA 2 cut(s) 278, 407
AvaI CYCGRG 4 cut(s) 114, 284, 407, 427
AvaII GGWCC 2 cut(s) 68, 271
AxyI CCTNAGG 1 cut(s) 275
BauI CACGAG 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 214
BbvI GCAGC 1 cut(s) 46
BcoDI GTCTC 3 cut(s) 101, 124, 392
BfaI CTAG 1 cut(s) 14
BfuAI ACCTGC 1 cut(s) 321
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
BmcAI AGTACT 1 cut(s) 384
Bme18I GGWCC 2 cut(s) 68, 271
BmeT110I CYCGRG 4 cut(s) 114, 284, 407, 427
BmgT120I GGNCC 2 cut(s) 68, 271
BplI GAGNNNNNCTC 2 cut(s) 108, 140
BsaI GGTCTC 1 cut(s) 392
BsaJI CCNNGG 2 cut(s) 371, 408
Bse1I ACTGG 1 cut(s) 460
Bse21I CCTNAGG 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 371, 408
BseGI GGATG 1 cut(s) 99
BseNI ACTGG 1 cut(s) 460
BseXI GCAGC 1 cut(s) 46
BshFI GGCC 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 214
BsiHKCI CYCGRG 4 cut(s) 114, 284, 407, 427
BsiSI CCGG 2 cut(s) 340, 360
BslFI GGGAC 1 cut(s) 97
BsmAI GTCTC 3 cut(s) 101, 124, 392
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 339
Bso31I GGTCTC 1 cut(s) 392
BsoBI CYCGRG 4 cut(s) 114, 284, 407, 427
Bsp1286I GDGCHC 1 cut(s) 214
Bsp143I GATC 1 cut(s) 184
BspANI GGCC 1 cut(s) 339
BspMI ACCTGC 1 cut(s) 321
BspTNI GGTCTC 1 cut(s) 392
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 371, 408
BssMI GATC 1 cut(s) 184
BssSI CACGAG 1 cut(s) 213
Bst2BI CACGAG 1 cut(s) 213
Bst6I CTCTTC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 224, 275
BstDSI CCRYGG 1 cut(s) 371
BstF5I GGATG 1 cut(s) 99
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 3 cut(s) 101, 124, 392
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 46
Bsu36I CCTNAGG 1 cut(s) 275
BsuRI GGCC 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 371
BtsCI GGATG 1 cut(s) 99
BveI ACCTGC 1 cut(s) 321
CfoI GCGC 1 cut(s) 420
Cfr13I GGNCC 2 cut(s) 68, 271
Csp6I GTAC 2 cut(s) 149, 383
CviJI RGCY 4 cut(s) 59, 262, 283, 339
CviKI_1 RGCY 4 cut(s) 59, 262, 283, 339
CviQI GTAC 2 cut(s) 149, 383
DdeI CTNAG 2 cut(s) 224, 275
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 133
Eco31I GGTCTC 1 cut(s) 392
Eco47I GGWCC 2 cut(s) 68, 271
Eco81I CCTNAGG 1 cut(s) 275
Eco88I CYCGRG 4 cut(s) 114, 284, 407, 427
EcoO109I RGGNCCY 1 cut(s) 271
FaiI YATR 3 cut(s) 204, 239, 457
FaqI GGGAC 1 cut(s) 97
Fnu4HI GCNGC 1 cut(s) 60
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 1 cut(s) 60
FspBI CTAG 1 cut(s) 14
GlaI GCGC 1 cut(s) 419
GluI GCNGC 1 cut(s) 60
HaeIII GGCC 1 cut(s) 339
HapII CCGG 2 cut(s) 340, 360
HhaI GCGC 1 cut(s) 420
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 260
HinfI GANTC 1 cut(s) 117
HpaII CCGG 2 cut(s) 340, 360
HphI GGTGA 2 cut(s) 278, 407
Hpy166II GTNNAC 1 cut(s) 149
Hpy188III TCNNGA 3 cut(s) 114, 215, 407
Hpy8I GTNNAC 1 cut(s) 149
HpyAV CCTTC 3 cut(s) 142, 295, 459
HpyCH4IV ACGT 2 cut(s) 177, 312
HpyCH4V TGCA 2 cut(s) 62, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 336
HpyF3I CTNAG 2 cut(s) 224, 275
HpySE526I ACGT 2 cut(s) 177, 312
HspAI GCGC 1 cut(s) 418
Kzo9I GATC 1 cut(s) 184
LpnPI CCDG 4 cut(s) 122, 316, 353, 373
Lsp1109I GCAGC 1 cut(s) 46
MaeI CTAG 1 cut(s) 14
MaeII ACGT 2 cut(s) 177, 312
MaeIII GTNAC 2 cut(s) 422, 440
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 3 cut(s) 120, 127, 130
MhlI GDGCHC 1 cut(s) 214
MluCI AATT 2 cut(s) 163, 197
MlyI GAGTC 1 cut(s) 126
MnlI CCTC 6 cut(s) 113, 182, 211, 262, 403, 423
MroXI GAANNNNTTC 1 cut(s) 163
MslI CAYNNNNRTG 2 cut(s) 207, 460
MspI CCGG 2 cut(s) 340, 360
MwoI GCNNNNNNNGC 1 cut(s) 336
NdeII GATC 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 422
PaeR7I CTCGAG 1 cut(s) 427
PdmI GAANNNNTTC 1 cut(s) 163
PkrI GCNGC 1 cut(s) 61
PleI GAGTC 1 cut(s) 125
PpsI GAGTC 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 271
Psp5II RGGWCCY 1 cut(s) 271
PspPI GGNCC 2 cut(s) 68, 271
PspPPI RGGWCCY 1 cut(s) 271
PspXI VCTCGAGB 1 cut(s) 427
RsaI GTAC 2 cut(s) 150, 384
RsaNI GTAC 2 cut(s) 149, 383
RseI CAYNNNNRTG 2 cut(s) 207, 460
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 1 cut(s) 184
Sau96I GGNCC 2 cut(s) 68, 271
ScaI AGTACT 1 cut(s) 384
SchI GAGTC 1 cut(s) 126
SduI GDGCHC 1 cut(s) 214
SetI ASST 9 cut(s) 180, 222, 264, 273, 285, 306, 315, 335, 404
Sfr274I CTCGAG 1 cut(s) 427
SinI GGWCC 2 cut(s) 68, 271
SlaI CTCGAG 1 cut(s) 427
SmiMI CAYNNNNRTG 2 cut(s) 207, 460
SmlI CTYRAG 1 cut(s) 427
SmoI CTYRAG 1 cut(s) 427
Sse9I AATT 2 cut(s) 163, 197
SspMI CTAG 1 cut(s) 14
TaiI ACGT 2 cut(s) 180, 315
TaqI TCGA 3 cut(s) 171, 187, 428
TasI AATT 2 cut(s) 163, 197
TatI WGTACW 1 cut(s) 382
TseFI GTSAC 1 cut(s) 422
TseI GCWGC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 422
TspDTI ATGAA 1 cut(s) 130
TspGWI ACGGA 2 cut(s) 166, 360
VpaK11BI GGWCC 2 cut(s) 68, 271
XhoI CTCGAG 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 163
XspI CTAG 1 cut(s) 14
ZrmI AGTACT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.