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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 47 |
| AarI | CACCTGC | 1 cut(s) | 1372 |
| Acc16I | TGCGCA | 1 cut(s) | 1252 |
| Acc36I | ACCTGC | 2 cut(s) | 630, 1372 |
| AccB1I | GGYRCC | 1 cut(s) | 740 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 751 |
| AccII | CGCG | 4 cut(s) | 917, 940, 1392, 1715 |
| AciI | CCGC | 5 cut(s) | 799, 915, 935, 1317, 1390 |
| AclWI | GGATC | 1 cut(s) | 1773 |
| AcoI | YGGCCR | 1 cut(s) | 922 |
| AcsI | RAATTY | 7 cut(s) | 67, 155, 652, 683, 772, 1882, 2012 |
| AcvI | CACGTG | 4 cut(s) | 520, 897, 910, 1414 |
| AcyI | GRCGYC | 2 cut(s) | 5, 868 |
| AfaI | GTAC | 2 cut(s) | 1353, 1489 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 246, 751, 920, 931, 1047, 1326, 1327 |
| AflIII | ACRYGT | 5 cut(s) | 517, 894, 896, 1552, 1999 |
| AgsI | TTSAA | 8 cut(s) | 91, 154, 414, 697, 1600, 2012, 2089, 2132 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1665 |
| AjnI | CCWGG | 3 cut(s) | 622, 902, 1500 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1874, 1906 |
| AleI | CACNNNNGTG | 3 cut(s) | 332, 897, 1003 |
| AluBI | AGCT | 9 cut(s) | 405, 469, 561, 582, 730, 1285, 1473, 1605, 1625 |
| AluI | AGCT | 9 cut(s) | 405, 469, 561, 582, 730, 1285, 1473, 1605, 1625 |
| Alw21I | GWGCWC | 6 cut(s) | 471, 506, 1058, 1287, 1315, 1475 |
| Alw26I | GTCTC | 2 cut(s) | 805, 1030 |
| Alw44I | GTGCAC | 1 cut(s) | 502 |
| AlwI | GGATC | 1 cut(s) | 1773 |
| Ama87I | CYCGRG | 1 cut(s) | 1065 |
| AoxI | GGCC | 3 cut(s) | 181, 620, 922 |
| ApaLI | GTGCAC | 1 cut(s) | 502 |
| ApeKI | GCWGC | 3 cut(s) | 221, 802, 1248 |
| ApoI | RAATTY | 7 cut(s) | 67, 155, 652, 683, 772, 1882, 2012 |
| AspLEI | GCGC | 4 cut(s) | 919, 940, 1253, 1717 |
| AspS9I | GGNCC | 3 cut(s) | 181, 1417, 1775 |
| AsuC2I | CCSGG | 2 cut(s) | 1779, 2026 |
| AsuHPI | GGTGA | 5 cut(s) | 203, 682, 808, 1396, 1847 |
| AsuNHI | GCTAGC | 1 cut(s) | 1975 |
| AvaI | CYCGRG | 1 cut(s) | 1065 |
| AvaII | GGWCC | 2 cut(s) | 1417, 1775 |
| AxyI | CCTNAGG | 1 cut(s) | 1046 |
| BaeGI | GKGCMC | 3 cut(s) | 506, 915, 1333 |
| BanI | GGYRCC | 1 cut(s) | 740 |
| BanII | GRGCYC | 3 cut(s) | 471, 1287, 1475 |
| BauI | CACGAG | 1 cut(s) | 1637 |
| BbrPI | CACGTG | 4 cut(s) | 520, 897, 910, 1414 |
| BbsI | GAAGAC | 2 cut(s) | 831, 1400 |
| Bbv12I | GWGCWC | 6 cut(s) | 471, 506, 1058, 1287, 1315, 1475 |
| BbvI | GCAGC | 3 cut(s) | 208, 789, 1235 |
| BccI | CCATC | 3 cut(s) | 848, 1049, 1642 |
| BceAI | ACGGC | 3 cut(s) | 803, 881, 1470 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 218, 252, 1292, 1326 |
| BciT130I | CCWGG | 3 cut(s) | 624, 904, 1502 |
| BcnI | CCSGG | 2 cut(s) | 1779, 2026 |
| BcoDI | GTCTC | 2 cut(s) | 805, 1030 |
| BfaI | CTAG | 2 cut(s) | 1476, 1976 |
| BfmI | CTRYAG | 1 cut(s) | 1362 |
| BfuAI | ACCTGC | 2 cut(s) | 630, 1372 |
| BisI | GCNGC | 5 cut(s) | 222, 800, 803, 936, 1249 |
| BlsI | GCNGC | 5 cut(s) | 223, 801, 804, 937, 1250 |
| Bme1390I | CCNGG | 5 cut(s) | 624, 904, 1502, 1779, 2026 |
| Bme18I | GGWCC | 2 cut(s) | 1417, 1775 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1665 |
| BmeT110I | CYCGRG | 1 cut(s) | 1065 |
| BmgT120I | GGNCC | 3 cut(s) | 181, 1417, 1775 |
| BmiI | GGNNCC | 3 cut(s) | 490, 742, 1372 |
| BmrFI | CCNGG | 5 cut(s) | 624, 904, 1502, 1779, 2026 |
| BmrI | ACTGGG | 1 cut(s) | 1442 |
| BmsI | GCATC | 3 cut(s) | 1263, 1689, 1962 |
| BmtI | GCTAGC | 1 cut(s) | 1979 |
| BmuI | ACTGGG | 1 cut(s) | 1442 |
| BpiI | GAAGAC | 2 cut(s) | 831, 1400 |
| BpmI | CTGGAG | 1 cut(s) | 645 |
| Bpu10I | CCTNAGC | 1 cut(s) | 470 |
| BpuEI | CTTGAG | 2 cut(s) | 1448, 1489 |
| BpuMI | CCSGG | 2 cut(s) | 1779, 2026 |
| BsaAI | YACGTR | 4 cut(s) | 520, 897, 910, 1414 |
| BsaHI | GRCGYC | 2 cut(s) | 5, 868 |
| BsaI | GGTCTC | 1 cut(s) | 1030 |
| BsaJI | CCNNGG | 5 cut(s) | 241, 1501, 1778, 1980, 2024 |
| BsaWI | WCCGGW | 2 cut(s) | 667, 818 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 246, 751, 920, 931, 1047, 1326, 1327 |
| Bse118I | RCCGGY | 1 cut(s) | 924 |
| Bse1I | ACTGG | 2 cut(s) | 1404, 1448 |
| Bse21I | CCTNAGG | 1 cut(s) | 1046 |
| Bse3DI | GCAATG | 2 cut(s) | 797, 1192 |
| BseBI | CCWGG | 3 cut(s) | 624, 904, 1502 |
| BseDI | CCNNGG | 5 cut(s) | 241, 1501, 1778, 1980, 2024 |
| BseGI | GGATG | 6 cut(s) | 14, 981, 1041, 1969, 1977, 2046 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 246, 751, 920, 931, 1047, 1326, 1327 |
| BseMI | GCAATG | 2 cut(s) | 797, 1192 |
| BseMII | CTCAG | 2 cut(s) | 484, 1037 |
| BseNI | ACTGG | 2 cut(s) | 1404, 1448 |
| BseSI | GKGCMC | 3 cut(s) | 506, 915, 1333 |
| BseXI | GCAGC | 3 cut(s) | 208, 789, 1235 |
| BsgI | GTGCAG | 1 cut(s) | 1279 |
| Bsh1236I | CGCG | 4 cut(s) | 917, 940, 1392, 1715 |
| BshFI | GGCC | 3 cut(s) | 183, 622, 924 |
| BshNI | GGYRCC | 1 cut(s) | 740 |
| BsiHKAI | GWGCWC | 6 cut(s) | 471, 506, 1058, 1287, 1315, 1475 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1065 |
| BsiSI | CCGG | 5 cut(s) | 668, 819, 925, 1778, 2026 |
| BslFI | GGGAC | 1 cut(s) | 1478 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 246, 751, 920, 931, 1047, 1326, 1327 |
| BsmAI | GTCTC | 2 cut(s) | 805, 1030 |
| BsmFI | GGGAC | 1 cut(s) | 1478 |
| BsmI | GAATGC | 2 cut(s) | 1810, 2065 |
| BsnI | GGCC | 3 cut(s) | 183, 622, 924 |
| Bso31I | GGTCTC | 1 cut(s) | 1030 |
| BsoBI | CYCGRG | 1 cut(s) | 1065 |
| Bsp1286I | GDGCHC | 8 cut(s) | 471, 506, 915, 1058, 1287, 1315, 1333, 1475 |
| Bsp143I | GATC | 3 cut(s) | 522, 995, 1765 |
| BspACI | CCGC | 5 cut(s) | 799, 915, 935, 1317, 1390 |
| BspANI | GGCC | 3 cut(s) | 183, 622, 924 |
| BspCNI | CTCAG | 2 cut(s) | 483, 1038 |
| BspFNI | CGCG | 4 cut(s) | 917, 940, 1392, 1715 |
| BspLI | GGNNCC | 3 cut(s) | 490, 742, 1372 |
| BspMI | ACCTGC | 2 cut(s) | 630, 1372 |
| BspOI | GCTAGC | 1 cut(s) | 1979 |
| BspPI | GGATC | 1 cut(s) | 1773 |
| BspT107I | GGYRCC | 1 cut(s) | 740 |
| BspTNI | GGTCTC | 1 cut(s) | 1030 |
| BsrDI | GCAATG | 2 cut(s) | 797, 1192 |
| BsrFI | RCCGGY | 1 cut(s) | 924 |
| BsrI | ACTGG | 2 cut(s) | 1404, 1448 |
| BssAI | RCCGGY | 1 cut(s) | 924 |
| BssECI | CCNNGG | 5 cut(s) | 241, 1501, 1778, 1980, 2024 |
| BssMI | GATC | 3 cut(s) | 522, 995, 1765 |
| BssNI | GRCGYC | 2 cut(s) | 5, 868 |
| BssSI | CACGAG | 1 cut(s) | 1637 |
| BssT1I | CCWWGG | 2 cut(s) | 241, 1980 |
| Bst2BI | CACGAG | 1 cut(s) | 1637 |
| Bst2UI | CCWGG | 3 cut(s) | 624, 904, 1502 |
| Bst4CI | ACNGT | 5 cut(s) | 35, 97, 508, 1060, 1335 |
| Bst6I | CTCTTC | 1 cut(s) | 361 |
| BstACI | GRCGYC | 2 cut(s) | 5, 868 |
| BstBAI | YACGTR | 4 cut(s) | 520, 897, 910, 1414 |
| BstC8I | GCNNGC | 5 cut(s) | 807, 915, 926, 1255, 1977 |
| BstDEI | CTNAG | 3 cut(s) | 470, 546, 1046 |
| BstEII | GGTNACC | 1 cut(s) | 1384 |
| BstF5I | GGATG | 6 cut(s) | 14, 981, 1041, 1969, 1977, 2046 |
| BstFNI | CGCG | 4 cut(s) | 917, 940, 1392, 1715 |
| BstHHI | GCGC | 4 cut(s) | 919, 940, 1253, 1717 |
| BstKTI | GATC | 3 cut(s) | 525, 998, 1768 |
| BstMAI | GTCTC | 2 cut(s) | 805, 1030 |
| BstMBI | GATC | 3 cut(s) | 522, 995, 1765 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 796, 925, 1254, 1282 |
| BstNI | CCWGG | 3 cut(s) | 624, 904, 1502 |
| BstNSI | RCATGY | 2 cut(s) | 1556, 2003 |
| BstPI | GGTNACC | 1 cut(s) | 1384 |
| BstSCI | CCNGG | 5 cut(s) | 622, 902, 1500, 1777, 2024 |
| BstSFI | CTRYAG | 1 cut(s) | 1362 |
| BstSLI | GKGCMC | 3 cut(s) | 506, 915, 1333 |
| BstUI | CGCG | 4 cut(s) | 917, 940, 1392, 1715 |
| BstV1I | GCAGC | 3 cut(s) | 208, 789, 1235 |
| BstV2I | GAAGAC | 2 cut(s) | 831, 1400 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1046 |
| BsuRI | GGCC | 3 cut(s) | 183, 622, 924 |
| BtsCI | GGATG | 6 cut(s) | 14, 981, 1041, 1969, 1977, 2046 |
| BtsIMutI | CAGTG | 3 cut(s) | 504, 772, 1105 |
| BveI | ACCTGC | 2 cut(s) | 630, 1372 |
| Cac8I | GCNNGC | 5 cut(s) | 807, 915, 926, 1255, 1977 |
| CfoI | GCGC | 4 cut(s) | 919, 940, 1253, 1717 |
| Cfr10I | RCCGGY | 1 cut(s) | 924 |
| Cfr13I | GGNCC | 3 cut(s) | 181, 1417, 1775 |
| CseI | GACGC | 2 cut(s) | 13, 857 |
| Csp6I | GTAC | 2 cut(s) | 1352, 1488 |
| CviQI | GTAC | 2 cut(s) | 1352, 1488 |
| DdeI | CTNAG | 3 cut(s) | 470, 546, 1046 |
| DpnI | GATC | 3 cut(s) | 524, 997, 1767 |
| DpnII | GATC | 3 cut(s) | 522, 995, 1765 |
| DraI | TTTAAA | 4 cut(s) | 66, 964, 1182, 1822 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1665 |
| EaeI | YGGCCR | 1 cut(s) | 922 |
| Eam1104I | CTCTTC | 1 cut(s) | 361 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1665 |
| EarI | CTCTTC | 1 cut(s) | 361 |
| Ecl136II | GAGCTC | 3 cut(s) | 469, 1285, 1473 |
| Eco130I | CCWWGG | 2 cut(s) | 241, 1980 |
| Eco147I | AGGCCT | 1 cut(s) | 622 |
| Eco24I | GRGCYC | 3 cut(s) | 471, 1287, 1475 |
| Eco31I | GGTCTC | 1 cut(s) | 1030 |
| Eco47I | GGWCC | 2 cut(s) | 1417, 1775 |
| Eco53kI | GAGCTC | 3 cut(s) | 469, 1285, 1473 |
| Eco72I | CACGTG | 4 cut(s) | 520, 897, 910, 1414 |
| Eco81I | CCTNAGG | 1 cut(s) | 1046 |
| Eco88I | CYCGRG | 1 cut(s) | 1065 |
| Eco91I | GGTNACC | 1 cut(s) | 1384 |
| EcoICRI | GAGCTC | 3 cut(s) | 469, 1285, 1473 |
| EcoO109I | RGGNCCY | 1 cut(s) | 181 |
| EcoO65I | GGTNACC | 1 cut(s) | 1384 |
| EcoRI | GAATTC | 2 cut(s) | 772, 2012 |
| EcoRII | CCWGG | 3 cut(s) | 622, 902, 1500 |
| EcoT14I | CCWWGG | 2 cut(s) | 241, 1980 |
| EcoT22I | ATGCAT | 2 cut(s) | 1194, 1219 |
| EcoT38I | GRGCYC | 3 cut(s) | 471, 1287, 1475 |
| ErhI | CCWWGG | 2 cut(s) | 241, 1980 |
| FaqI | GGGAC | 1 cut(s) | 1478 |
| FauI | CCCGC | 3 cut(s) | 922, 1324, 1397 |
| Fnu4HI | GCNGC | 5 cut(s) | 222, 800, 803, 936, 1249 |
| FokI | GGATG | 5 cut(s) | 988, 1028, 1976, 1984, 2053 |
| FriOI | GRGCYC | 3 cut(s) | 471, 1287, 1475 |
| Fsp4HI | GCNGC | 5 cut(s) | 222, 800, 803, 936, 1249 |
| FspBI | CTAG | 2 cut(s) | 1476, 1976 |
| FspI | TGCGCA | 1 cut(s) | 1252 |
| GlaI | GCGC | 4 cut(s) | 918, 939, 1252, 1716 |
| GluI | GCNGC | 5 cut(s) | 222, 800, 803, 936, 1249 |
| GsuI | CTGGAG | 1 cut(s) | 645 |
| HaeIII | GGCC | 3 cut(s) | 183, 622, 924 |
| HapII | CCGG | 5 cut(s) | 668, 819, 925, 1778, 2026 |
| HgaI | GACGC | 2 cut(s) | 13, 857 |
| HhaI | GCGC | 4 cut(s) | 919, 940, 1253, 1717 |
| Hin1I | GRCGYC | 2 cut(s) | 5, 868 |
| Hin6I | GCGC | 4 cut(s) | 917, 938, 1251, 1715 |
| HinP1I | GCGC | 4 cut(s) | 917, 938, 1251, 1715 |
| HincII | GTYRAC | 1 cut(s) | 292 |
| HindII | GTYRAC | 1 cut(s) | 292 |
| HindIII | AAGCTT | 3 cut(s) | 559, 728, 1603 |
| HpaII | CCGG | 5 cut(s) | 668, 819, 925, 1778, 2026 |
| HphI | GGTGA | 5 cut(s) | 203, 682, 808, 1396, 1847 |
| Hpy166II | GTNNAC | 4 cut(s) | 31, 292, 504, 1636 |
| Hpy188I | TCNGA | 5 cut(s) | 367, 586, 1375, 1549, 1659 |
| Hpy8I | GTNNAC | 4 cut(s) | 31, 292, 504, 1636 |
| Hpy99I | CGWCG | 1 cut(s) | 233 |
| HpyAV | CCTTC | 4 cut(s) | 472, 612, 1097, 1348 |
| HpyCH4III | ACNGT | 5 cut(s) | 35, 97, 508, 1060, 1335 |
| HpyCH4IV | ACGT | 6 cut(s) | 519, 896, 909, 1000, 1413, 1666 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 796, 925, 1254, 1282 |
| HpyF3I | CTNAG | 3 cut(s) | 470, 546, 1046 |
| HpySE526I | ACGT | 6 cut(s) | 519, 896, 909, 1000, 1413, 1666 |
| Hsp92I | GRCGYC | 2 cut(s) | 5, 868 |
| HspAI | GCGC | 4 cut(s) | 917, 938, 1251, 1715 |
| KroI | GCCGGC | 1 cut(s) | 924 |
| KroNI | GCCGGC | 1 cut(s) | 926 |
| Kzo9I | GATC | 3 cut(s) | 522, 995, 1765 |
| LmnI | GCTCC | 1 cut(s) | 1318 |
| Lsp1109I | GCAGC | 3 cut(s) | 208, 789, 1235 |
| LweI | GCATC | 3 cut(s) | 1263, 1689, 1962 |
| MaeI | CTAG | 2 cut(s) | 1476, 1976 |
| MaeII | ACGT | 6 cut(s) | 519, 896, 909, 1000, 1413, 1666 |
| MaeIII | GTNAC | 9 cut(s) | 334, 434, 541, 1060, 1384, 1493, 1505, 1667, 2002 |
| MalI | GATC | 3 cut(s) | 524, 997, 1767 |
| MboI | GATC | 3 cut(s) | 522, 995, 1765 |
| MboII | GAAGA | 5 cut(s) | 179, 348, 612, 836, 1400 |
| MfeI | CAATTG | 1 cut(s) | 479 |
| MhlI | GDGCHC | 8 cut(s) | 471, 506, 915, 1058, 1287, 1315, 1333, 1475 |
| MlyI | GAGTC | 7 cut(s) | 287, 331, 451, 484, 807, 1370, 1839 |
| MmeI | TCCRAC | 2 cut(s) | 930, 1398 |
| Mph1103I | ATGCAT | 2 cut(s) | 1194, 1219 |
| MroNI | GCCGGC | 1 cut(s) | 924 |
| MslI | CAYNNNNRTG | 5 cut(s) | 262, 332, 897, 1003, 1098 |
| MspI | CCGG | 5 cut(s) | 668, 819, 925, 1778, 2026 |
| MspR9I | CCNGG | 5 cut(s) | 624, 904, 1502, 1779, 2026 |
| MunI | CAATTG | 1 cut(s) | 479 |
| Mva1269I | GAATGC | 2 cut(s) | 1810, 2065 |
| MvaI | CCWGG | 3 cut(s) | 624, 904, 1502 |
| MvnI | CGCG | 4 cut(s) | 917, 940, 1392, 1715 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 796, 925, 1254, 1282 |
| NaeI | GCCGGC | 1 cut(s) | 926 |
| NciI | CCSGG | 2 cut(s) | 1779, 2026 |
| NdeII | GATC | 3 cut(s) | 522, 995, 1765 |
| NgoMIV | GCCGGC | 1 cut(s) | 924 |
| NheI | GCTAGC | 1 cut(s) | 1975 |
| NlaIV | GGNNCC | 3 cut(s) | 490, 742, 1372 |
| NmuCI | GTSAC | 6 cut(s) | 334, 434, 1060, 1384, 1493, 1667 |
| NsbI | TGCGCA | 1 cut(s) | 1252 |
| NsiI | ATGCAT | 2 cut(s) | 1194, 1219 |
| NspI | RCATGY | 2 cut(s) | 1556, 2003 |
| OliI | CACNNNNGTG | 3 cut(s) | 332, 897, 1003 |
| PaeR7I | CTCGAG | 1 cut(s) | 1065 |
| PaqCI | CACCTGC | 1 cut(s) | 1372 |
| PceI | AGGCCT | 1 cut(s) | 622 |
| PciI | ACATGT | 2 cut(s) | 1552, 1999 |
| PctI | GAATGC | 2 cut(s) | 1810, 2065 |
| PdiI | GCCGGC | 1 cut(s) | 926 |
| PfeI | GAWTC | 4 cut(s) | 430, 675, 1918, 2115 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 751 |
| PkrI | GCNGC | 5 cut(s) | 223, 801, 804, 937, 1250 |
| PleI | GAGTC | 7 cut(s) | 287, 331, 451, 483, 807, 1370, 1839 |
| PmaCI | CACGTG | 4 cut(s) | 520, 897, 910, 1414 |
| PmlI | CACGTG | 4 cut(s) | 520, 897, 910, 1414 |
| PpsI | GAGTC | 7 cut(s) | 287, 331, 451, 483, 807, 1370, 1839 |
| Ppu21I | YACGTR | 4 cut(s) | 520, 897, 910, 1414 |
| PscI | ACATGT | 2 cut(s) | 1552, 1999 |
| PsiI | TTATAA | 1 cut(s) | 47 |
| Psp124BI | GAGCTC | 3 cut(s) | 471, 1287, 1475 |
| Psp6I | CCWGG | 3 cut(s) | 622, 902, 1500 |
| PspCI | CACGTG | 4 cut(s) | 520, 897, 910, 1414 |
| PspEI | GGTNACC | 1 cut(s) | 1384 |
| PspGI | CCWGG | 3 cut(s) | 622, 902, 1500 |
| PspN4I | GGNNCC | 3 cut(s) | 490, 742, 1372 |
| PspPI | GGNCC | 3 cut(s) | 181, 1417, 1775 |
| PspXI | VCTCGAGB | 1 cut(s) | 1065 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1753, 1785 |
| RsaI | GTAC | 2 cut(s) | 1353, 1489 |
| RsaNI | GTAC | 2 cut(s) | 1352, 1488 |
| RseI | CAYNNNNRTG | 5 cut(s) | 262, 332, 897, 1003, 1098 |
| SacI | GAGCTC | 3 cut(s) | 471, 1287, 1475 |
| SatI | GCNGC | 5 cut(s) | 222, 800, 803, 936, 1249 |
| Sau3AI | GATC | 3 cut(s) | 522, 995, 1765 |
| Sau96I | GGNCC | 3 cut(s) | 181, 1417, 1775 |
| SchI | GAGTC | 7 cut(s) | 287, 331, 451, 484, 807, 1370, 1839 |
| ScrFI | CCNGG | 5 cut(s) | 624, 904, 1502, 1779, 2026 |
| SduI | GDGCHC | 8 cut(s) | 471, 506, 915, 1058, 1287, 1315, 1333, 1475 |
| SfaNI | GCATC | 3 cut(s) | 1263, 1689, 1962 |
| SfcI | CTRYAG | 1 cut(s) | 1362 |
| Sfr274I | CTCGAG | 1 cut(s) | 1065 |
| SinI | GGWCC | 2 cut(s) | 1417, 1775 |
| SlaI | CTCGAG | 1 cut(s) | 1065 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 262, 332, 897, 1003, 1098 |
| SmlI | CTYRAG | 3 cut(s) | 1065, 1427, 1468 |
| SmoI | CTYRAG | 3 cut(s) | 1065, 1427, 1468 |
| SseBI | AGGCCT | 1 cut(s) | 622 |
| SsiI | CCGC | 5 cut(s) | 799, 915, 935, 1317, 1390 |
| SspI | AATATT | 2 cut(s) | 836, 1169 |
| SspMI | CTAG | 2 cut(s) | 1476, 1976 |
| SstI | GAGCTC | 3 cut(s) | 471, 1287, 1475 |
| StuI | AGGCCT | 1 cut(s) | 622 |
| StyD4I | CCNGG | 5 cut(s) | 622, 902, 1500, 1777, 2024 |
| StyI | CCWWGG | 2 cut(s) | 241, 1980 |
| TaaI | ACNGT | 5 cut(s) | 35, 97, 508, 1060, 1335 |
| TaiI | ACGT | 6 cut(s) | 522, 899, 912, 1003, 1416, 1669 |
| TaqI | TCGA | 5 cut(s) | 228, 465, 776, 1066, 1560 |
| TauI | GCSGC | 2 cut(s) | 802, 938 |
| TfiI | GAWTC | 4 cut(s) | 430, 675, 1918, 2115 |
| TscAI | CASTG | 3 cut(s) | 511, 772, 1105 |
| TseFI | GTSAC | 6 cut(s) | 334, 434, 1060, 1384, 1493, 1667 |
| TseI | GCWGC | 3 cut(s) | 221, 802, 1248 |
| Tsp45I | GTSAC | 6 cut(s) | 334, 434, 1060, 1384, 1493, 1667 |
| TspGWI | ACGGA | 2 cut(s) | 867, 1110 |
| TspRI | CASTG | 3 cut(s) | 511, 772, 1105 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 751 |
| VneI | GTGCAC | 1 cut(s) | 502 |
| VpaK11BI | GGWCC | 2 cut(s) | 1417, 1775 |
| XapI | RAATTY | 7 cut(s) | 67, 155, 652, 683, 772, 1882, 2012 |
| XbaI | TCTAGA | 1 cut(s) | 1475 |
| XceI | RCATGY | 2 cut(s) | 1556, 2003 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1411 |
| XhoI | CTCGAG | 1 cut(s) | 1065 |
| XspI | CTAG | 2 cut(s) | 1476, 1976 |
| Zsp2I | ATGCAT | 2 cut(s) | 1194, 1219 |