pycom520g00530

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000513
Physical Location & Seq
Forward (+)
446604 .. 448334
1731 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00530.5

Sequence Viewer

Length: 1290 bp
ATGCATTTTGACCTCATTATCCTGCACCCCGGTGTTAGTGCTCCAACCCATGGCCAGGGCACAATCCTTCATATGATGTTCACCTCCCGCATCGTACGCTCACCTTGGATCCAAGTTTGGTTATTCGCACAGTCTTCACGTGATCGTAGCACTCGAGCGTACTTATTTACACCTAGCCCTATCGTACAGACCACAATAGGTGATTCCGACTCGTGTGCAGGCATATCTGATGAATTGGCATACTATTCTACATTGATTCGTGGCATACCTCTATTTTCACTACTTCTAAGCATTGTTTTTATATACCTACGTGTTATTATTTTCTGGGAAATTATACGGGGTTTACACTTTGTTTTTGCCCACTCACGTTTTCGGTTTTACGCCCCTCCAGGTTCTAGGTATACAATTTTGCCCCTAGTTCGATTTTTGTCACGTTGTGGGTTGTTTGGAGTGTTGGGGCATGTGTTTGGAACCTTACACACTCCCACACACACACTGACACTGCTGTCTCTCTCTCCCTCTGTCTTCTCCCTGTCTTTCTCTCCCCCGAGCTCCCATTGCCACTGTATACGTACGGACACACACCAAGAACCATTATTTCGTAGCAGATCGAGGAAACCAAGGCCTTTTCAGGTGAGAACATCGACGTTTTCACGTCGATTTCTTAAACTCCGTTTTGAGTACTGTTCATGTTCCCAGGAGCTTGGGAGTGGCTCGTTGGTTGAATTTGGACGTAAGGATCCTTGGTTGTGGAGTTGGCCGGTTTTGGGAAGCTTGCACAGGTATAATCTATTTAGAGCTTTAAATTGGTGCAAGAAACGTGGAAAAAGGTGGAAAAACGAGAGAGTTAGAGCGATTTGCAGGTTTCCGGCGACGGTGCCGGCGACGGCGACGGTGCCGGCGACGGCGACGGTGCCGGCGTCGGGGGGCTGGCCGGAGAAGAAGCAGGAATATTCTGTTAAGTTTAACGGAATATTCCTGACGGCAGGGGTTAAGTTTGACGGAATATTCCTGACGGCGTCTGTTGACACCGTCAGTGTGCCTGGCACGTGGCCGCGCGTGGGGGCGCGTGGGTCTGTGCCTGTGCTGGCGCGTGGGGGAGCGTGCGGGAGCACGTTGGTATATTCATTTGTCCATTTTGAGCAATGTATGAGAAGTTTTTACGAGAAACGGGTTATGTGCTTTAAAATTAACGTTTTTATAGTTGTTTCGCATTTAGGTGACACGTACCCCGAGGACGAGCGTGCACACGCAAGACAGGGGGGCTACGACCCTTCTACATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

48.59

Weight (kDa)

10.01

Isoelectric Point (pI)

48.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 505
Acc36I ACCTGC 1 cut(s) 852
AccB1I GGYRCC 3 cut(s) 877, 895, 913
AccB7I CCANNNNNTGG 1 cut(s) 50
AccI GTMKAC 2 cut(s) 401, 568
AccII CGCG 4 cut(s) 1057, 1059, 1069, 1093
AciI CCGC 3 cut(s) 88, 1055, 1107
AclI AACGTT 1 cut(s) 1194
AclWI GGATC 4 cut(s) 103, 116, 734, 747
AcoI YGGCCR 4 cut(s) 52, 758, 932, 1052
AcsI RAATTY 1 cut(s) 725
AcvI CACGTG 2 cut(s) 140, 1050
AcyI GRCGYC 2 cut(s) 920, 1019
AdeI CACNNNGTG 1 cut(s) 437
AfaI GTAC 6 cut(s) 96, 161, 186, 574, 683, 1229
AfiI CCNNNNNNNGG 5 cut(s) 50, 55, 767, 923, 1061
AflIII ACRYGT 2 cut(s) 310, 1224
AgsI TTSAA 1 cut(s) 725
AjiI CACGTC 1 cut(s) 656
AjnI CCWGG 4 cut(s) 54, 388, 696, 1042
AleI CACNNNNGTG 1 cut(s) 30
AluBI AGCT 4 cut(s) 552, 703, 774, 800
AluI AGCT 4 cut(s) 552, 703, 774, 800
Alw21I GWGCWC 4 cut(s) 43, 554, 1115, 1249
Alw26I GTCTC 1 cut(s) 513
Alw44I GTGCAC 1 cut(s) 1245
AlwI GGATC 4 cut(s) 103, 116, 734, 747
Ama87I CYCGRG 3 cut(s) 153, 547, 1232
AoxI GGCC 5 cut(s) 52, 623, 758, 932, 1052
ApaLI GTGCAC 1 cut(s) 1245
ApoI RAATTY 1 cut(s) 725
AspLEI GCGC 3 cut(s) 1059, 1069, 1093
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 5 cut(s) 73, 93, 212, 646, 1232
AvaI CYCGRG 3 cut(s) 153, 547, 1232
BaeGI GKGCMC 2 cut(s) 62, 1249
BalI TGGCCA 1 cut(s) 54
BamHI GGATCC 2 cut(s) 108, 739
BanI GGYRCC 3 cut(s) 877, 895, 913
BanII GRGCYC 1 cut(s) 554
BauI CACGAG 1 cut(s) 211
BbrPI CACGTG 2 cut(s) 140, 1050
BbsI GAAGAC 2 cut(s) 126, 517
Bbv12I GWGCWC 4 cut(s) 43, 554, 1115, 1249
BceAI ACGGC 4 cut(s) 903, 921, 999, 1032
BcgI CGANNNNNNTGC 2 cut(s) 197, 231
BciT130I CCWGG 4 cut(s) 56, 390, 698, 1044
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 513
BfaI CTAG 3 cut(s) 174, 396, 416
BfuAI ACCTGC 1 cut(s) 852
BisI GCNGC 1 cut(s) 1055
BlsI GCNGC 1 cut(s) 1056
BmcAI AGTACT 1 cut(s) 683
Bme1390I CCNGG 5 cut(s) 30, 56, 390, 698, 1044
BmeT110I CYCGRG 3 cut(s) 153, 547, 1232
BmgBI CACGTC 1 cut(s) 656
BmiI GGNNCC 6 cut(s) 110, 472, 741, 879, 897, 915
BmrFI CCNGG 5 cut(s) 30, 56, 390, 698, 1044
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 126, 517
BpmI CTGGAG 1 cut(s) 372
BpuMI CCSGG 1 cut(s) 30
BsaAI YACGTR 5 cut(s) 140, 311, 572, 1050, 1227
BsaHI GRCGYC 2 cut(s) 920, 1019
BsaJI CCNNGG 8 cut(s) 28, 49, 55, 104, 620, 696, 743, 1233
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 55, 767, 923, 1061
Bse118I RCCGGY 4 cut(s) 760, 880, 898, 916
Bse1I ACTGG 1 cut(s) 1285
Bse3DI GCAATG 2 cut(s) 556, 1151
BseBI CCWGG 4 cut(s) 56, 390, 698, 1044
BseDI CCNNGG 8 cut(s) 28, 49, 55, 104, 620, 696, 743, 1233
BseLI CCNNNNNNNGG 5 cut(s) 50, 55, 767, 923, 1061
BseMI GCAATG 2 cut(s) 556, 1151
BseNI ACTGG 1 cut(s) 1285
BseSI GKGCMC 2 cut(s) 62, 1249
BsgI GTGCAG 2 cut(s) 8, 237
Bsh1236I CGCG 4 cut(s) 1057, 1059, 1069, 1093
BshFI GGCC 5 cut(s) 54, 625, 760, 934, 1054
BshNI GGYRCC 3 cut(s) 877, 895, 913
BsiHKAI GWGCWC 4 cut(s) 43, 554, 1115, 1249
BsiHKCI CYCGRG 3 cut(s) 153, 547, 1232
BsiSI CCGG 7 cut(s) 30, 761, 869, 881, 899, 917, 935
BsiWI CGTACG 2 cut(s) 94, 572
BslI CCNNNNNNNGG 5 cut(s) 50, 55, 767, 923, 1061
BsmAI GTCTC 1 cut(s) 513
BsnI GGCC 5 cut(s) 54, 625, 760, 934, 1054
BsoBI CYCGRG 3 cut(s) 153, 547, 1232
Bsp1286I GDGCHC 5 cut(s) 43, 62, 554, 1115, 1249
Bsp143I GATC 4 cut(s) 108, 142, 608, 739
Bsp19I CCATGG 1 cut(s) 49
BspACI CCGC 3 cut(s) 88, 1055, 1107
BspANI GGCC 5 cut(s) 54, 625, 760, 934, 1054
BspFNI CGCG 4 cut(s) 1057, 1059, 1069, 1093
BspLI GGNNCC 6 cut(s) 110, 472, 741, 879, 897, 915
BspMI ACCTGC 1 cut(s) 852
BspPI GGATC 4 cut(s) 103, 116, 734, 747
BspT107I GGYRCC 3 cut(s) 877, 895, 913
BsrDI GCAATG 2 cut(s) 556, 1151
BsrFI RCCGGY 4 cut(s) 760, 880, 898, 916
BsrI ACTGG 1 cut(s) 1285
BssAI RCCGGY 4 cut(s) 760, 880, 898, 916
BssECI CCNNGG 8 cut(s) 28, 49, 55, 104, 620, 696, 743, 1233
BssMI GATC 4 cut(s) 108, 142, 608, 739
BssNAI GTATAC 2 cut(s) 402, 569
BssNI GRCGYC 2 cut(s) 920, 1019
BssSI CACGAG 1 cut(s) 211
BssT1I CCWWGG 4 cut(s) 49, 104, 620, 743
Bst1107I GTATAC 2 cut(s) 402, 569
Bst2BI CACGAG 1 cut(s) 211
Bst2UI CCWGG 4 cut(s) 56, 390, 698, 1044
Bst4CI ACNGT 7 cut(s) 132, 566, 686, 877, 895, 913, 1033
BstACI GRCGYC 2 cut(s) 920, 1019
BstBAI YACGTR 5 cut(s) 140, 311, 572, 1050, 1227
BstC8I GCNNGC 9 cut(s) 220, 776, 882, 900, 918, 932, 1089, 1105, 1245
BstDEI CTNAG 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 49
BstFNI CGCG 4 cut(s) 1057, 1059, 1069, 1093
BstHHI GCGC 3 cut(s) 1059, 1069, 1093
BstKTI GATC 4 cut(s) 111, 145, 611, 742
BstMAI GTCTC 1 cut(s) 513
BstMBI GATC 4 cut(s) 108, 142, 608, 739
BstMWI GCNNNNNNNGC 2 cut(s) 96, 558
BstNI CCWGG 4 cut(s) 56, 390, 698, 1044
BstNSI RCATGY 1 cut(s) 464
BstSCI CCNGG 5 cut(s) 28, 54, 388, 696, 1042
BstSLI GKGCMC 2 cut(s) 62, 1249
BstSNI TACGTA 1 cut(s) 572
BstUI CGCG 4 cut(s) 1057, 1059, 1069, 1093
BstV2I GAAGAC 2 cut(s) 126, 517
BstX2I RGATCY 2 cut(s) 108, 739
BstXI CCANNNNNNTGG 1 cut(s) 704
BstYI RGATCY 2 cut(s) 108, 739
BstZ17I GTATAC 2 cut(s) 402, 569
BsuRI GGCC 5 cut(s) 54, 625, 760, 934, 1054
BtgI CCRYGG 1 cut(s) 49
BtrI CACGTC 1 cut(s) 656
BtsI GCAGTG 1 cut(s) 500
BtsIMutI CAGTG 4 cut(s) 494, 500, 562, 1042
BveI ACCTGC 1 cut(s) 852
Cac8I GCNNGC 9 cut(s) 220, 776, 882, 900, 918, 932, 1089, 1105, 1245
CfoI GCGC 3 cut(s) 1059, 1069, 1093
Cfr10I RCCGGY 4 cut(s) 760, 880, 898, 916
CseI GACGC 2 cut(s) 909, 1008
Csp6I GTAC 6 cut(s) 95, 160, 185, 573, 682, 1228
CspCI CAANNNNNGTGG 2 cut(s) 802, 837
CviAII CATG 3 cut(s) 50, 461, 690
CviQI GTAC 6 cut(s) 95, 160, 185, 573, 682, 1228
DdeI CTNAG 1 cut(s) 287
DpnI GATC 4 cut(s) 110, 144, 610, 741
DpnII GATC 4 cut(s) 108, 142, 608, 739
DraI TTTAAA 2 cut(s) 804, 1186
DraIII CACNNNGTG 1 cut(s) 437
DrdI GACNNNNNNGTC 1 cut(s) 505
DseDI GACNNNNNNGTC 1 cut(s) 505
EaeI YGGCCR 4 cut(s) 52, 758, 932, 1052
Ecl136II GAGCTC 1 cut(s) 552
Eco105I TACGTA 1 cut(s) 572
Eco130I CCWWGG 4 cut(s) 49, 104, 620, 743
Eco147I AGGCCT 1 cut(s) 625
Eco24I GRGCYC 1 cut(s) 554
Eco53kI GAGCTC 1 cut(s) 552
Eco72I CACGTG 2 cut(s) 140, 1050
Eco88I CYCGRG 3 cut(s) 153, 547, 1232
EcoICRI GAGCTC 1 cut(s) 552
EcoRII CCWGG 4 cut(s) 54, 388, 696, 1042
EcoT14I CCWWGG 4 cut(s) 49, 104, 620, 743
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 554
ErhI CCWWGG 4 cut(s) 49, 104, 620, 743
FaeI CATG 3 cut(s) 53, 464, 693
FatI CATG 3 cut(s) 49, 460, 689
FauI CCCGC 2 cut(s) 95, 1100
FauNDI CATATG 1 cut(s) 72
FblI GTMKAC 2 cut(s) 401, 568
Fnu4HI GCNGC 1 cut(s) 1055
FriOI GRGCYC 1 cut(s) 554
Fsp4HI GCNGC 1 cut(s) 1055
FspBI CTAG 3 cut(s) 174, 396, 416
GlaI GCGC 3 cut(s) 1058, 1068, 1092
GluI GCNGC 1 cut(s) 1055
GsuI CTGGAG 1 cut(s) 372
HaeIII GGCC 5 cut(s) 54, 625, 760, 934, 1054
HapII CCGG 7 cut(s) 30, 761, 869, 881, 899, 917, 935
HgaI GACGC 2 cut(s) 909, 1008
HhaI GCGC 3 cut(s) 1059, 1069, 1093
Hin1I GRCGYC 2 cut(s) 920, 1019
Hin1II CATG 3 cut(s) 53, 464, 693
Hin6I GCGC 3 cut(s) 1057, 1067, 1091
HinP1I GCGC 3 cut(s) 1057, 1067, 1091
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HindIII AAGCTT 1 cut(s) 772
HinfI GANTC 3 cut(s) 203, 209, 256
HpaII CCGG 7 cut(s) 30, 761, 869, 881, 899, 917, 935
HphI GGTGA 5 cut(s) 73, 93, 212, 646, 1232
Hpy166II GTNNAC 6 cut(s) 81, 344, 402, 569, 1027, 1247
Hpy188I TCNGA 2 cut(s) 208, 229
Hpy188III TCNNGA 2 cut(s) 979, 1012
Hpy8I GTNNAC 6 cut(s) 81, 344, 402, 569, 1027, 1247
Hpy99I CGWCG 8 cut(s) 649, 660, 877, 889, 895, 907, 913, 925
HpyAV CCTTC 2 cut(s) 77, 1284
HpyCH4III ACNGT 7 cut(s) 132, 566, 686, 877, 895, 913, 1033
HpyCH4V TGCA 7 cut(s) 4, 25, 218, 778, 813, 861, 1247
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 558
HpyF3I CTNAG 1 cut(s) 287
Hsp92I GRCGYC 2 cut(s) 920, 1019
Hsp92II CATG 3 cut(s) 53, 464, 693
HspAI GCGC 3 cut(s) 1057, 1067, 1091
KroI GCCGGC 3 cut(s) 880, 898, 916
KroNI GCCGGC 3 cut(s) 882, 900, 918
Kzo9I GATC 4 cut(s) 108, 142, 608, 739
LmnI GCTCC 5 cut(s) 46, 557, 700, 1100, 1110
LweI GCATC 1 cut(s) 99
MaeI CTAG 3 cut(s) 174, 396, 416
MaeIII GTNAC 2 cut(s) 429, 1220
MalI GATC 4 cut(s) 110, 144, 610, 741
MboI GATC 4 cut(s) 108, 142, 608, 739
MboII GAAGA 3 cut(s) 126, 517, 952
MflI RGATCY 2 cut(s) 108, 739
MhlI GDGCHC 5 cut(s) 43, 62, 554, 1115, 1249
MlsI TGGCCA 1 cut(s) 54
MluCI AATT 6 cut(s) 233, 330, 405, 725, 805, 1188
MluNI TGGCCA 1 cut(s) 54
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 2 cut(s) 68, 231
MnlI CCTC 7 cut(s) 23, 94, 279, 396, 529, 606, 1228
Mox20I TGGCCA 1 cut(s) 54
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 3 cut(s) 880, 898, 916
MscI TGGCCA 1 cut(s) 54
MseI TTAA 7 cut(s) 666, 803, 960, 966, 993, 1185, 1191
MslI CAYNNNNRTG 3 cut(s) 30, 1218, 1285
Msp20I TGGCCA 1 cut(s) 54
MspI CCGG 7 cut(s) 30, 761, 869, 881, 899, 917, 935
MspR9I CCNGG 5 cut(s) 30, 56, 390, 698, 1044
MvaI CCWGG 4 cut(s) 56, 390, 698, 1044
MvnI CGCG 4 cut(s) 1057, 1059, 1069, 1093
MwoI GCNNNNNNNGC 2 cut(s) 96, 558
NaeI GCCGGC 3 cut(s) 882, 900, 918
NciI CCSGG 1 cut(s) 30
NcoI CCATGG 1 cut(s) 49
NdeI CATATG 1 cut(s) 72
NdeII GATC 4 cut(s) 108, 142, 608, 739
NgoMIV GCCGGC 3 cut(s) 880, 898, 916
NlaIII CATG 3 cut(s) 53, 464, 693
NlaIV GGNNCC 6 cut(s) 110, 472, 741, 879, 897, 915
NmuCI GTSAC 2 cut(s) 429, 1220
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 464
OliI CACNNNNGTG 1 cut(s) 30
PaeR7I CTCGAG 1 cut(s) 153
PceI AGGCCT 1 cut(s) 625
PcsI WCGNNNNNNNCGW 4 cut(s) 151, 881, 899, 917
PdiI GCCGGC 3 cut(s) 882, 900, 918
PfeI GAWTC 2 cut(s) 203, 256
Pfl23II CGTACG 2 cut(s) 94, 572
PflFI GACNNNGTC 2 cut(s) 1018, 1031
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 1 cut(s) 1056
PleI GAGTC 1 cut(s) 203
PmaCI CACGTG 2 cut(s) 140, 1050
PmlI CACGTG 2 cut(s) 140, 1050
PpsI GAGTC 1 cut(s) 203
Ppu21I YACGTR 5 cut(s) 140, 311, 572, 1050, 1227
Psp124BI GAGCTC 1 cut(s) 554
Psp1406I AACGTT 1 cut(s) 1194
Psp6I CCWGG 4 cut(s) 54, 388, 696, 1042
PspCI CACGTG 2 cut(s) 140, 1050
PspGI CCWGG 4 cut(s) 54, 388, 696, 1042
PspLI CGTACG 2 cut(s) 94, 572
PspN4I GGNNCC 6 cut(s) 110, 472, 741, 879, 897, 915
PspXI VCTCGAGB 1 cut(s) 153
PsuI RGATCY 2 cut(s) 108, 739
PsyI GACNNNGTC 2 cut(s) 1018, 1031
RsaI GTAC 6 cut(s) 96, 161, 186, 574, 683, 1229
RsaNI GTAC 6 cut(s) 95, 160, 185, 573, 682, 1228
RseI CAYNNNNRTG 3 cut(s) 30, 1218, 1285
SacI GAGCTC 1 cut(s) 554
SaqAI TTAA 7 cut(s) 666, 803, 960, 966, 993, 1185, 1191
SatI GCNGC 1 cut(s) 1055
Sau3AI GATC 4 cut(s) 108, 142, 608, 739
ScaI AGTACT 1 cut(s) 683
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 5 cut(s) 30, 56, 390, 698, 1044
SduI GDGCHC 5 cut(s) 43, 62, 554, 1115, 1249
SfaNI GCATC 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 153
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 3 cut(s) 30, 1218, 1285
SmlI CTYRAG 1 cut(s) 153
SmoI CTYRAG 1 cut(s) 153
SnaBI TACGTA 1 cut(s) 572
Sse9I AATT 6 cut(s) 233, 330, 405, 725, 805, 1188
SseBI AGGCCT 1 cut(s) 625
SsiI CCGC 3 cut(s) 88, 1055, 1107
SspI AATATT 3 cut(s) 953, 975, 1008
SspMI CTAG 3 cut(s) 174, 396, 416
SstI GAGCTC 1 cut(s) 554
StuI AGGCCT 1 cut(s) 625
StyD4I CCNGG 5 cut(s) 28, 54, 388, 696, 1042
StyI CCWWGG 4 cut(s) 49, 104, 620, 743
TaaI ACNGT 7 cut(s) 132, 566, 686, 877, 895, 913, 1033
TaqI TCGA 5 cut(s) 154, 421, 611, 644, 658
TasI AATT 6 cut(s) 233, 330, 405, 725, 805, 1188
TatI WGTACW 1 cut(s) 681
TauI GCSGC 1 cut(s) 1057
TfiI GAWTC 2 cut(s) 203, 256
Tru1I TTAA 7 cut(s) 666, 803, 960, 966, 993, 1185, 1191
Tru9I TTAA 7 cut(s) 666, 803, 960, 966, 993, 1185, 1191
TscAI CASTG 4 cut(s) 501, 507, 569, 1042
TseFI GTSAC 2 cut(s) 429, 1220
Tsp45I GTSAC 2 cut(s) 429, 1220
TspDTI ATGAA 4 cut(s) 59, 246, 678, 1116
TspGWI ACGGA 4 cut(s) 590, 662, 984, 1017
TspRI CASTG 4 cut(s) 501, 507, 569, 1042
Tth111I GACNNNGTC 2 cut(s) 1018, 1031
Van91I CCANNNNNTGG 1 cut(s) 50
VneI GTGCAC 1 cut(s) 1245
XapI RAATTY 1 cut(s) 725
XceI RCATGY 1 cut(s) 464
XhoI CTCGAG 1 cut(s) 153
XmiI GTMKAC 2 cut(s) 401, 568
XspI CTAG 3 cut(s) 174, 396, 416
ZrmI AGTACT 1 cut(s) 683
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.