pycom17g16310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000513
Physical Location & Seq
Forward (+)
14485030 .. 14486757
1728 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g16310.12

Sequence Viewer

Length: 1149 bp
ATGATAACTGTAACTTCTTATTCATCAGAAAATTGCAACCAGATGTTAATTAACCTGAATAAACTGTGTTTTACTATTTTTCGTGATTCACGCTTAATAGTAGTGTTCAAGACACGAAGACAAATTTCAACATCCAATAACAGTATCTCATTTGTTTGTGGACAAGTGTTAGCCACAGTTGAAGTCGAAACAATGGCACATCACCATCTCATAGTTTCATGTTCATATTGCATCAATAAAGGTACACAAATTGAGTTAGGCAGAGAAGTAGGACCCAAACATTTGTTTATTATACCCTTCACATTTTTAACTTCAGTTCCTATAAATTCGATTTGTCTATTCTGTCACTCCATTAATTCATATAAGAGTTTTTATCATAAATGGTCATTGAAATTGATTCTCATCATTAAGATGGTGTCTCAATGTGGTCATTCTGTCACAATTCTGTTAAAAAATCTGTTAAGTGCTAATGTGGCACATAAATGGCCCCATAAGTTTTTTTTTTTTGGGTTTTCGACCGTCAGATCGGATGAATTGAAGAAGATCAACGAACATAAATTATCAAGGGGTTCCTTGAAATTGAACCGTGACATGAAAATGGTCCCTAAAATTGACATGCGACATCAAAATGTTCAGGGCTTGAAAGTGGTAAGGTTTTGTTCTACGTGTTGTAAGCTTCATTTTGGTAAAATTTTCCAGTTTTCCGGCGACAGCGACGGCGCTAGAGGTTGGCCGGAGAAGACGACGGAATATTCCGTCAGTTTGGACGGAATATTCCTAACGGCTCCTGACGATGTTAACTGGCGCCGTCAGCGTGCCAGGCACGTGCATGCGCGTTGGCGGCCCGTGTGGCCGTGCCTTAGCCGGCGTGTGGGGGCACGTGTGATGTCGAAAAATTATTTTAAAAATATGGGGATGTTCGTGAGGTTGAGTAGATCACGTTGGTGTATTCATAAACCCCATTTGATTGTTTCGCATATAGGTGAGACCTATCCTGAGGACGAGCGCATGGGCTTTTGGTTTTCCGTATATACCTATATACTTGTGATTTTCCCAAAAAATGAAATGAAATGTTTATATGTTTTAAATGCCATGCATTGCATTTGCCTATTTATTTATGCATTAGTAGTTGCATATATGTACGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

383

Amino Acids

44.45

Weight (kDa)

9.68

Isoelectric Point (pI)

42.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 804
AccII CGCG 1 cut(s) 835
AciI CCGC 1 cut(s) 841
AcoI YGGCCR 2 cut(s) 731, 851
AcsI RAATTY 3 cut(s) 123, 325, 690
AcuI CTGAAG 1 cut(s) 297
AcvI CACGTG 2 cut(s) 826, 881
AcyI GRCGYC 1 cut(s) 805
AfaI GTAC 2 cut(s) 244, 1142
AfiI CCNNNNNNNGG 1 cut(s) 871
AflIII ACRYGT 2 cut(s) 665, 880
AgsI TTSAA 8 cut(s) 109, 129, 182, 391, 538, 577, 583, 643
AjnI CCWGG 1 cut(s) 818
AleI CACNNNNGTG 1 cut(s) 943
AluBI AGCT 1 cut(s) 676
AluI AGCT 1 cut(s) 676
Alw26I GTCTC 2 cut(s) 423, 980
AoxI GGCC 4 cut(s) 485, 731, 842, 851
ApoI RAATTY 3 cut(s) 123, 325, 690
AseI ATTAAT 1 cut(s) 354
AspLEI GCGC 4 cut(s) 722, 807, 835, 1008
AspS9I GGNCC 4 cut(s) 272, 486, 601, 843
AsuHPI GGTGA 2 cut(s) 194, 995
AvaII GGWCC 2 cut(s) 272, 601
AxyI CCTNAGG 1 cut(s) 996
BaeGI GKGCMC 1 cut(s) 880
BanI GGYRCC 1 cut(s) 804
BbrPI CACGTG 2 cut(s) 826, 881
BbsI GAAGAC 2 cut(s) 124, 746
BccI CCATC 2 cut(s) 213, 406
BceAI ACGGC 4 cut(s) 733, 792, 798, 838
BciT130I CCWGG 1 cut(s) 820
BcoDI GTCTC 2 cut(s) 423, 980
BfaI CTAG 1 cut(s) 723
BfoI RGCGCY 2 cut(s) 723, 808
BglI GCCNNNNNGGC 1 cut(s) 850
BisI GCNGC 1 cut(s) 842
BlsI GCNGC 1 cut(s) 843
Bme1390I CCNGG 1 cut(s) 820
Bme18I GGWCC 2 cut(s) 272, 601
BmgT120I GGNCC 4 cut(s) 272, 486, 601, 843
BmiI GGNNCC 6 cut(s) 274, 488, 571, 603, 786, 806
BmrFI CCNGG 1 cut(s) 820
BmsI GCATC 1 cut(s) 240
BpiI GAAGAC 2 cut(s) 124, 746
Bpu10I CCTNAGC 1 cut(s) 860
BsaAI YACGTR 3 cut(s) 666, 826, 881
BsaBI GATNNNNATC 1 cut(s) 401
BsaHI GRCGYC 1 cut(s) 805
BsaI GGTCTC 1 cut(s) 980
Bsc4I CCNNNNNNNGG 1 cut(s) 871
Bse118I RCCGGY 1 cut(s) 864
Bse1I ACTGG 2 cut(s) 697, 806
Bse21I CCTNAGG 1 cut(s) 996
Bse3DI GCAATG 1 cut(s) 1096
Bse8I GATNNNNATC 1 cut(s) 401
BseBI CCWGG 1 cut(s) 820
BseGI GGATG 3 cut(s) 131, 535, 921
BseJI GATNNNNATC 1 cut(s) 401
BseLI CCNNNNNNNGG 1 cut(s) 871
BseMI GCAATG 1 cut(s) 1096
BseMII CTCAG 1 cut(s) 987
BseNI ACTGG 2 cut(s) 697, 806
BseSI GKGCMC 1 cut(s) 880
Bsh1236I CGCG 1 cut(s) 835
Bsh1285I CGRYCG 1 cut(s) 519
BshFI GGCC 4 cut(s) 487, 733, 844, 853
BshNI GGYRCC 1 cut(s) 804
BsiEI CGRYCG 1 cut(s) 519
BsiSI CCGG 3 cut(s) 705, 734, 865
BslFI GGGAC 1 cut(s) 587
BslI CCNNNNNNNGG 1 cut(s) 871
BsmAI GTCTC 2 cut(s) 423, 980
BsmFI GGGAC 1 cut(s) 587
BsnI GGCC 4 cut(s) 487, 733, 844, 853
Bso31I GGTCTC 1 cut(s) 980
Bsp1286I GDGCHC 1 cut(s) 880
Bsp143I GATC 3 cut(s) 524, 543, 935
BspACI CCGC 1 cut(s) 841
BspANI GGCC 4 cut(s) 487, 733, 844, 853
BspCNI CTCAG 1 cut(s) 988
BspFNI CGCG 1 cut(s) 835
BspLI GGNNCC 6 cut(s) 274, 488, 571, 603, 786, 806
BspT107I GGYRCC 1 cut(s) 804
BspTNI GGTCTC 1 cut(s) 980
BsrDI GCAATG 1 cut(s) 1096
BsrFI RCCGGY 1 cut(s) 864
BsrI ACTGG 2 cut(s) 697, 806
BssAI RCCGGY 1 cut(s) 864
BssMI GATC 3 cut(s) 524, 543, 935
BssNI GRCGYC 1 cut(s) 805
Bst2UI CCWGG 1 cut(s) 820
Bst4CI ACNGT 6 cut(s) 10, 66, 143, 178, 520, 587
BstACI GRCGYC 1 cut(s) 805
BstBAI YACGTR 3 cut(s) 666, 826, 881
BstC8I GCNNGC 3 cut(s) 816, 831, 866
BstDEI CTNAG 2 cut(s) 860, 996
BstF5I GGATG 3 cut(s) 131, 535, 921
BstFNI CGCG 1 cut(s) 835
BstH2I RGCGCY 2 cut(s) 723, 808
BstHHI GCGC 4 cut(s) 722, 807, 835, 1008
BstKTI GATC 3 cut(s) 527, 546, 938
BstMAI GTCTC 2 cut(s) 423, 980
BstMBI GATC 3 cut(s) 524, 543, 935
BstMCI CGRYCG 1 cut(s) 519
BstMWI GCNNNNNNNGC 5 cut(s) 473, 811, 820, 841, 850
BstNI CCWGG 1 cut(s) 820
BstNSI RCATGY 2 cut(s) 619, 833
BstSCI CCNGG 1 cut(s) 818
BstSLI GKGCMC 1 cut(s) 880
BstUI CGCG 1 cut(s) 835
BstV2I GAAGAC 2 cut(s) 124, 746
Bsu36I CCTNAGG 1 cut(s) 996
BsuRI GGCC 4 cut(s) 487, 733, 844, 853
BtsCI GGATG 3 cut(s) 131, 535, 921
Cac8I GCNNGC 3 cut(s) 816, 831, 866
CfoI GCGC 4 cut(s) 722, 807, 835, 1008
Cfr10I RCCGGY 1 cut(s) 864
Cfr13I GGNCC 4 cut(s) 272, 486, 601, 843
Csp6I GTAC 2 cut(s) 243, 1141
CviAII CATG 6 cut(s) 219, 592, 616, 830, 1009, 1093
CviQI GTAC 2 cut(s) 243, 1141
DdeI CTNAG 2 cut(s) 860, 996
DinI GGCGCC 1 cut(s) 806
DpnI GATC 3 cut(s) 526, 545, 937
DpnII GATC 3 cut(s) 524, 543, 935
DraI TTTAAA 2 cut(s) 904, 1086
EaeI YGGCCR 2 cut(s) 731, 851
Eco31I GGTCTC 1 cut(s) 980
Eco47I GGWCC 2 cut(s) 272, 601
Eco57I CTGAAG 1 cut(s) 297
Eco72I CACGTG 2 cut(s) 826, 881
Eco81I CCTNAGG 1 cut(s) 996
EcoO109I RGGNCCY 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 818
EcoT22I ATGCAT 2 cut(s) 1098, 1123
EgeI GGCGCC 1 cut(s) 806
EheI GGCGCC 1 cut(s) 806
FaeI CATG 6 cut(s) 222, 595, 619, 833, 1012, 1096
FaqI GGGAC 1 cut(s) 587
FatI CATG 6 cut(s) 218, 591, 615, 829, 1008, 1092
Fnu4HI GCNGC 1 cut(s) 842
FokI GGATG 3 cut(s) 118, 542, 928
Fsp4HI GCNGC 1 cut(s) 842
FspBI CTAG 1 cut(s) 723
GlaI GCGC 4 cut(s) 721, 806, 834, 1007
GluI GCNGC 1 cut(s) 842
HaeII RGCGCY 2 cut(s) 723, 808
HaeIII GGCC 4 cut(s) 487, 733, 844, 853
HapII CCGG 3 cut(s) 705, 734, 865
HhaI GCGC 4 cut(s) 722, 807, 835, 1008
Hin1I GRCGYC 1 cut(s) 805
Hin1II CATG 6 cut(s) 222, 595, 619, 833, 1012, 1096
Hin6I GCGC 4 cut(s) 720, 805, 833, 1006
HinP1I GCGC 4 cut(s) 720, 805, 833, 1006
HincII GTYRAC 1 cut(s) 799
HindII GTYRAC 1 cut(s) 799
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 2 cut(s) 86, 397
HpaI GTTAAC 1 cut(s) 799
HpaII CCGG 3 cut(s) 705, 734, 865
HphI GGTGA 2 cut(s) 194, 995
Hpy166II GTNNAC 3 cut(s) 161, 245, 799
Hpy188I TCNGA 3 cut(s) 28, 524, 529
Hpy188III TCNNGA 5 cut(s) 83, 109, 788, 922, 995
Hpy8I GTNNAC 3 cut(s) 161, 245, 799
Hpy99I CGWCG 2 cut(s) 719, 748
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 6 cut(s) 10, 66, 143, 178, 520, 587
HpyCH4IV ACGT 5 cut(s) 665, 825, 880, 940, 1143
HpyCH4V TGCA 7 cut(s) 36, 231, 829, 1096, 1101, 1121, 1133
HpyF10VI GCNNNNNNNGC 5 cut(s) 473, 811, 820, 841, 850
HpyF3I CTNAG 2 cut(s) 860, 996
HpySE526I ACGT 5 cut(s) 665, 825, 880, 940, 1143
Hsp92I GRCGYC 1 cut(s) 805
Hsp92II CATG 6 cut(s) 222, 595, 619, 833, 1012, 1096
HspAI GCGC 4 cut(s) 720, 805, 833, 1006
KasI GGCGCC 1 cut(s) 804
KroI GCCGGC 1 cut(s) 864
KroNI GCCGGC 1 cut(s) 866
KspAI GTTAAC 1 cut(s) 799
Kzo9I GATC 3 cut(s) 524, 543, 935
LmnI GCTCC 1 cut(s) 790
LweI GCATC 1 cut(s) 240
MaeI CTAG 1 cut(s) 723
MaeII ACGT 5 cut(s) 665, 825, 880, 940, 1143
MaeIII GTNAC 4 cut(s) 10, 344, 436, 587
MalI GATC 3 cut(s) 526, 545, 937
MboI GATC 3 cut(s) 524, 543, 935
MboII GAAGA 4 cut(s) 129, 550, 553, 751
MhlI GDGCHC 1 cut(s) 880
Mly113I GGCGCC 1 cut(s) 805
MnlI CCTC 3 cut(s) 719, 918, 991
Mph1103I ATGCAT 2 cut(s) 1098, 1123
MroNI GCCGGC 1 cut(s) 864
MslI CAYNNNNRTG 7 cut(s) 410, 481, 596, 627, 828, 943, 981
MspI CCGG 3 cut(s) 705, 734, 865
MspR9I CCNGG 1 cut(s) 820
MvaI CCWGG 1 cut(s) 820
MvnI CGCG 1 cut(s) 835
MwoI GCNNNNNNNGC 5 cut(s) 473, 811, 820, 841, 850
NaeI GCCGGC 1 cut(s) 866
NarI GGCGCC 1 cut(s) 805
NdeII GATC 3 cut(s) 524, 543, 935
NgoMIV GCCGGC 1 cut(s) 864
NlaIII CATG 6 cut(s) 222, 595, 619, 833, 1012, 1096
NlaIV GGNNCC 6 cut(s) 274, 488, 571, 603, 786, 806
NmuCI GTSAC 3 cut(s) 344, 436, 587
NsiI ATGCAT 2 cut(s) 1098, 1123
NspI RCATGY 2 cut(s) 619, 833
OliI CACNNNNGTG 1 cut(s) 943
PacI TTAATTAA 1 cut(s) 51
PaeI GCATGC 1 cut(s) 833
PdiI GCCGGC 1 cut(s) 866
PfeI GAWTC 2 cut(s) 86, 397
PkrI GCNGC 1 cut(s) 843
PluTI GGCGCC 1 cut(s) 808
PmaCI CACGTG 2 cut(s) 826, 881
PmlI CACGTG 2 cut(s) 826, 881
Ppu21I YACGTR 3 cut(s) 666, 826, 881
PpuMI RGGWCCY 1 cut(s) 272
PshBI ATTAAT 1 cut(s) 354
Psp5II RGGWCCY 1 cut(s) 272
Psp6I CCWGG 1 cut(s) 818
PspCI CACGTG 2 cut(s) 826, 881
PspGI CCWGG 1 cut(s) 818
PspN4I GGNNCC 6 cut(s) 274, 488, 571, 603, 786, 806
PspPI GGNCC 4 cut(s) 272, 486, 601, 843
PspPPI RGGWCCY 1 cut(s) 272
RsaI GTAC 2 cut(s) 244, 1142
RsaNI GTAC 2 cut(s) 243, 1141
RseI CAYNNNNRTG 7 cut(s) 410, 481, 596, 627, 828, 943, 981
SatI GCNGC 1 cut(s) 842
Sau3AI GATC 3 cut(s) 524, 543, 935
Sau96I GGNCC 4 cut(s) 272, 486, 601, 843
ScrFI CCNGG 1 cut(s) 820
SduI GDGCHC 1 cut(s) 880
SfaNI GCATC 1 cut(s) 240
SfiI GGCCNNNNNGGCC 1 cut(s) 850
SfoI GGCGCC 1 cut(s) 806
SinI GGWCC 2 cut(s) 272, 601
SmiMI CAYNNNNRTG 7 cut(s) 410, 481, 596, 627, 828, 943, 981
SphI GCATGC 1 cut(s) 833
SsiI CCGC 1 cut(s) 841
SspDI GGCGCC 1 cut(s) 804
SspI AATATT 2 cut(s) 752, 774
SspMI CTAG 1 cut(s) 723
StyD4I CCNGG 1 cut(s) 818
TaaI ACNGT 6 cut(s) 10, 66, 143, 178, 520, 587
TaiI ACGT 5 cut(s) 668, 828, 883, 943, 1146
TaqI TCGA 4 cut(s) 186, 329, 515, 890
TauI GCSGC 1 cut(s) 844
TfiI GAWTC 2 cut(s) 86, 397
TseFI GTSAC 3 cut(s) 344, 436, 587
Tsp45I GTSAC 3 cut(s) 344, 436, 587
TspGWI ACGGA 4 cut(s) 745, 761, 783, 1015
VpaK11BI GGWCC 2 cut(s) 272, 601
VspI ATTAAT 1 cut(s) 354
XapI RAATTY 3 cut(s) 123, 325, 690
XceI RCATGY 2 cut(s) 619, 833
XspI CTAG 1 cut(s) 723
Zsp2I ATGCAT 2 cut(s) 1098, 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.