pycom808g00600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000808
OG0000513
Physical Location & Seq
Forward (+)
379783 .. 382186
2404 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom808g00600.18

Sequence Viewer

Length: 1569 bp
ATGCGAAGGAGATTAGTAAAGGAAAATTTTAGTTTTTTCGGGAGTATGGTGTTATTGATTGTACATTTCACACCGTATTGTATGATTGAAAATGCTACGATACGGTTGAGTATTAGATTGCTTTCAATTGCAAAGGTGAACGCAGGACACCCAGGTAAGTTCAGTTATGTGTTAGGCAACTTCGATACTATCGTACTGCTCATAAACCTACACACCGGTGTTAGTGCTCCCGCCTGTGGCCAGGGCACAGTCCTTCACGTGATGTTCACCTTCTGCACCTTGCGCTTATCTTGGATTCAAGGTAGGTGCACAAACCTGTCTCTTCACGTGATCGTAGCACTTGAGCGTACTTATTTACACCTAGTCCTATCGTACAGACCACTAGAGGTGGTTCCAACTCGTGTGCAGGGATGTTTGATGAGCTATAGATTAAGTCGTACAGTGATGAGATATAAATACTTGCACCCCGGTGTTAGTTCACCTCCCACACCTTACGCTCACATTGGACTCAAGGTAGGTGCACAGGTGACCTGCGATTATTCGCCCAGTCTTCACGTGATCGTAGCACTTGAGCGTGCTTATTTACACCCAGTCCTGTCGTACAGACCACTAGAGGTGGTTCCGACTCGTGTGATTCTGGCTGATGTATCATTTTATATTGATTCAGTTCATTTGGCCTACTTGTTAATGCATTGTAGAGTCTATGGACACGGCATACTCGTGATATATACATATATATGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATGTGGGTTATTACTGTTAGAAAGGTAATAGTTTTGGGAAGCTTGGTTTTACTGCTCACTCACACTTTCTGTTTTGATATATGGCTAATCTTAGTATTGGTGGCTACTTTTAAGGGTATGATTCTTACCCATACTACTGTTCTTTATTTATGCTCTGACATCGCGTGTGAAATGAGTTCACTCCCACTCGTAGCGCACTCTGACACTATCACACTTTATGGCTTCGTCAGCTTCCAGGTGTCGGCCAACATAGCTCAATTTGGGGTCTTAGTGGACATTCAGGGTCGGGGTGTGTCAGAAAGAACTCTGGAATTCCCTAGCACTCCAATGGCAGATTCGGCACAATCCTTAGGTGCAGGTGCACGACACTCTTCCTCCTCTCCTTCCTTGCAAGCACAAATCACTCTTTTCCTCTCAGGAACTTGCGGCACTCTTTGTATTTTTTTCCTCTTCCCCCTTGAAAGTTGCGGCAATAGGTATGTTTTAGGACTCTTTAAATCAGATAGGAAGTGTTTTAATGTGATAAGGAATCCCCCTTATAAAGTTTTCCTTGTCTTCCAAGCCTCAATCATAATACTTCCAGATTGTAGCACACCCCTAGCCTTATTTGACCACCAAAAACGTCCATCCCAAGTGCTCCCCACGCATGTTATCCAATCCAAGTCCAAAACTGCTCCAAATTGCTCCACTTTGCCCTTTTTTGTCACCTTTGCCAGTTGGGCTTACAAACACATGAAAATAGAGCTAACAAAGTCTCATAAATATATTGATGAATCGAATACCTTAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

523

Amino Acids

58.25

Weight (kDa)

9.05

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1317
AarI CACCTGC 1 cut(s) 1124
Acc36I ACCTGC 2 cut(s) 539, 1124
AccII CGCG 1 cut(s) 941
AciI CCGC 3 cut(s) 231, 1203, 1245
AcoI YGGCCR 2 cut(s) 238, 1020
AcsI RAATTY 2 cut(s) 25, 1088
AcvI CACGTG 3 cut(s) 259, 328, 556
AfaI GTAC 6 cut(s) 63, 195, 349, 374, 439, 602
AfiI CCNNNNNNNGG 2 cut(s) 236, 1018
AgeI ACCGGT 1 cut(s) 215
AgsI TTSAA 4 cut(s) 89, 126, 299, 1238
AjnI CCWGG 3 cut(s) 151, 240, 1011
AjuI GAANNNNNNNTTGG 2 cut(s) 1096, 1128
AleI CACNNNNGTG 2 cut(s) 216, 468
AluBI AGCT 5 cut(s) 423, 819, 1008, 1031, 1522
AluI AGCT 5 cut(s) 423, 819, 1008, 1031, 1522
Alw21I GWGCWC 5 cut(s) 229, 311, 523, 1141, 1416
Alw26I GTCTC 2 cut(s) 324, 1536
Alw44I GTGCAC 3 cut(s) 307, 519, 1137
AoxI GGCC 3 cut(s) 238, 675, 1020
ApaLI GTGCAC 3 cut(s) 307, 519, 1137
ApoI RAATTY 2 cut(s) 25, 1088
AsiGI ACCGGT 1 cut(s) 215
AspLEI GCGC 2 cut(s) 285, 973
AsuC2I CCSGG 1 cut(s) 468
AsuHPI GGTGA 5 cut(s) 148, 259, 471, 538, 1474
AxyI CCTNAGG 1 cut(s) 1126
BaeGI GKGCMC 4 cut(s) 248, 311, 523, 1141
BaeI ACNNNNGTAYC 2 cut(s) 177, 210
BalI TGGCCA 1 cut(s) 240
BauI CACGAG 3 cut(s) 399, 627, 719
BbrPI CACGTG 3 cut(s) 259, 328, 556
BbsI GAAGAC 2 cut(s) 542, 1324
Bbv12I GWGCWC 5 cut(s) 229, 311, 523, 1141, 1416
BccI CCATC 1 cut(s) 1411
BceAI ACGGC 1 cut(s) 727
BciT130I CCWGG 3 cut(s) 153, 242, 1013
BcnI CCSGG 1 cut(s) 468
BcoDI GTCTC 2 cut(s) 324, 1536
BfaI CTAG 5 cut(s) 362, 383, 611, 1095, 1376
BfmI CTRYAG 1 cut(s) 424
BfuAI ACCTGC 2 cut(s) 539, 1124
BglI GCCNNNNNGGC 1 cut(s) 1496
BisI GCNGC 2 cut(s) 1204, 1246
BlsI GCNGC 2 cut(s) 1205, 1247
Bme1390I CCNGG 4 cut(s) 153, 242, 468, 1013
BmiI GGNNCC 2 cut(s) 393, 621
BmrFI CCNGG 4 cut(s) 153, 242, 468, 1013
BmrI ACTGGG 2 cut(s) 540, 584
BmuI ACTGGG 2 cut(s) 540, 584
BpiI GAAGAC 2 cut(s) 542, 1324
BpuEI CTTGAG 3 cut(s) 362, 494, 590
BpuMI CCSGG 1 cut(s) 468
BsaAI YACGTR 3 cut(s) 259, 328, 556
BsaJI CCNNGG 3 cut(s) 151, 241, 466
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 2 cut(s) 1136, 1166
Bsc4I CCNNNNNNNGG 2 cut(s) 236, 1018
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 3 cut(s) 546, 590, 1491
Bse21I CCTNAGG 1 cut(s) 1126
BseBI CCWGG 3 cut(s) 153, 242, 1013
BseDI CCNNGG 3 cut(s) 151, 241, 466
BseGI GGATG 2 cut(s) 416, 1403
BseLI CCNNNNNNNGG 2 cut(s) 236, 1018
BseMII CTCAG 1 cut(s) 1206
BseNI ACTGG 3 cut(s) 546, 590, 1491
BseRI GAGGAG 1 cut(s) 1144
BseSI GKGCMC 4 cut(s) 248, 311, 523, 1141
BsgI GTGCAG 3 cut(s) 259, 425, 1152
Bsh1236I CGCG 1 cut(s) 941
BshFI GGCC 3 cut(s) 240, 677, 1022
BshTI ACCGGT 1 cut(s) 215
BsiHKAI GWGCWC 5 cut(s) 229, 311, 523, 1141, 1416
BsiSI CCGG 2 cut(s) 216, 468
BslI CCNNNNNNNGG 2 cut(s) 236, 1018
BsmAI GTCTC 2 cut(s) 324, 1536
BsnI GGCC 3 cut(s) 240, 677, 1022
Bsp1286I GDGCHC 6 cut(s) 229, 248, 311, 523, 1141, 1416
Bsp1407I TGTACA 1 cut(s) 61
Bsp143I GATC 2 cut(s) 330, 558
BspACI CCGC 3 cut(s) 231, 1203, 1245
BspANI GGCC 3 cut(s) 240, 677, 1022
BspCNI CTCAG 1 cut(s) 1205
BspFNI CGCG 1 cut(s) 941
BspLI GGNNCC 2 cut(s) 393, 621
BspMI ACCTGC 2 cut(s) 539, 1124
BsrFI RCCGGY 1 cut(s) 215
BsrGI TGTACA 1 cut(s) 61
BsrI ACTGG 3 cut(s) 546, 590, 1491
BssAI RCCGGY 1 cut(s) 215
BssECI CCNNGG 3 cut(s) 151, 241, 466
BssMI GATC 2 cut(s) 330, 558
BssSI CACGAG 3 cut(s) 399, 627, 719
Bst2BI CACGAG 3 cut(s) 399, 627, 719
Bst2UI CCWGG 3 cut(s) 153, 242, 1013
Bst4CI ACNGT 6 cut(s) 75, 105, 250, 442, 793, 916
Bst6I CTCTTC 3 cut(s) 327, 1153, 1232
BstAUI TGTACA 1 cut(s) 61
BstBAI YACGTR 3 cut(s) 259, 328, 556
BstC8I GCNNGC 2 cut(s) 576, 1170
BstDEI CTNAG 4 cut(s) 868, 1045, 1126, 1192
BstEII GGTNACC 1 cut(s) 526
BstF5I GGATG 2 cut(s) 416, 1403
BstFNI CGCG 1 cut(s) 941
BstHHI GCGC 2 cut(s) 285, 973
BstKTI GATC 2 cut(s) 333, 561
BstMAI GTCTC 2 cut(s) 324, 1536
BstMBI GATC 2 cut(s) 330, 558
BstMWI GCNNNNNNNGC 6 cut(s) 282, 1005, 1028, 1115, 1420, 1496
BstNI CCWGG 3 cut(s) 153, 242, 1013
BstNSI RCATGY 1 cut(s) 1427
BstPI GGTNACC 1 cut(s) 526
BstSCI CCNGG 4 cut(s) 151, 240, 466, 1011
BstSFI CTRYAG 1 cut(s) 424
BstSLI GKGCMC 4 cut(s) 248, 311, 523, 1141
BstUI CGCG 1 cut(s) 941
BstV2I GAAGAC 2 cut(s) 542, 1324
Bsu36I CCTNAGG 1 cut(s) 1126
BsuRI GGCC 3 cut(s) 240, 677, 1022
BtgZI GCGATG 1 cut(s) 922
BtsCI GGATG 2 cut(s) 416, 1403
BtsIMutI CAGTG 1 cut(s) 447
BveI ACCTGC 2 cut(s) 539, 1124
Cac8I GCNNGC 2 cut(s) 576, 1170
CfoI GCGC 2 cut(s) 285, 973
Cfr10I RCCGGY 1 cut(s) 215
Csp6I GTAC 6 cut(s) 62, 194, 348, 373, 438, 601
CspAI ACCGGT 1 cut(s) 215
CviAII CATG 2 cut(s) 1424, 1510
CviQI GTAC 6 cut(s) 62, 194, 348, 373, 438, 601
DdeI CTNAG 4 cut(s) 868, 1045, 1126, 1192
DpnI GATC 2 cut(s) 332, 560
DpnII GATC 2 cut(s) 330, 558
DraI TTTAAA 1 cut(s) 1273
EaeI YGGCCR 2 cut(s) 238, 1020
Eam1104I CTCTTC 3 cut(s) 327, 1153, 1232
EarI CTCTTC 3 cut(s) 327, 1153, 1232
Eco72I CACGTG 3 cut(s) 259, 328, 556
Eco81I CCTNAGG 1 cut(s) 1126
Eco91I GGTNACC 1 cut(s) 526
EcoO65I GGTNACC 1 cut(s) 526
EcoRI GAATTC 1 cut(s) 1088
EcoRII CCWGG 3 cut(s) 151, 240, 1011
EcoT22I ATGCAT 1 cut(s) 693
FaeI CATG 2 cut(s) 1427, 1513
FalI AAGNNNNNCTT 2 cut(s) 1311, 1343
FatI CATG 2 cut(s) 1423, 1509
FauI CCCGC 1 cut(s) 238
Fnu4HI GCNGC 2 cut(s) 1204, 1246
FokI GGATG 2 cut(s) 423, 1390
Fsp4HI GCNGC 2 cut(s) 1204, 1246
FspBI CTAG 5 cut(s) 362, 383, 611, 1095, 1376
GlaI GCGC 2 cut(s) 284, 972
GluI GCNGC 2 cut(s) 1204, 1246
HaeIII GGCC 3 cut(s) 240, 677, 1022
HapII CCGG 2 cut(s) 216, 468
HhaI GCGC 2 cut(s) 285, 973
Hin1II CATG 2 cut(s) 1427, 1513
Hin6I GCGC 2 cut(s) 283, 971
HinP1I GCGC 2 cut(s) 283, 971
HindIII AAGCTT 1 cut(s) 817
HpaII CCGG 2 cut(s) 216, 468
HphI GGTGA 5 cut(s) 148, 259, 471, 538, 1474
Hpy166II GTNNAC 8 cut(s) 139, 267, 309, 479, 521, 956, 1051, 1139
Hpy188I TCNGA 5 cut(s) 624, 934, 979, 1075, 1279
Hpy188III TCNNGA 5 cut(s) 40, 721, 1085, 1194, 1358
Hpy8I GTNNAC 8 cut(s) 139, 267, 309, 479, 521, 956, 1051, 1139
HpyAV CCTTC 3 cut(s) 263, 280, 1170
HpyCH4III ACNGT 6 cut(s) 75, 105, 250, 442, 793, 916
HpyCH4IV ACGT 4 cut(s) 258, 327, 555, 1399
HpyF10VI GCNNNNNNNGC 6 cut(s) 282, 1005, 1028, 1115, 1420, 1496
HpyF3I CTNAG 4 cut(s) 868, 1045, 1126, 1192
HpySE526I ACGT 4 cut(s) 258, 327, 555, 1399
Hsp92II CATG 2 cut(s) 1427, 1513
HspAI GCGC 2 cut(s) 283, 971
Kzo9I GATC 2 cut(s) 330, 558
LmnI GCTCC 4 cut(s) 232, 1419, 1456, 1466
MaeI CTAG 5 cut(s) 362, 383, 611, 1095, 1376
MaeII ACGT 4 cut(s) 258, 327, 555, 1399
MaeIII GTNAC 2 cut(s) 526, 1480
MalI GATC 2 cut(s) 332, 560
MboI GATC 2 cut(s) 330, 558
MboII GAAGA 5 cut(s) 314, 542, 1140, 1219, 1324
MfeI CAATTG 1 cut(s) 126
MhlI GDGCHC 6 cut(s) 229, 248, 311, 523, 1141, 1416
MlsI TGGCCA 1 cut(s) 240
MluCI AATT 5 cut(s) 25, 126, 1034, 1088, 1456
MluNI TGGCCA 1 cut(s) 240
MlyI GAGTC 4 cut(s) 501, 619, 708, 1260
MmeI TCCRAC 2 cut(s) 419, 647
MnlI CCTC 8 cut(s) 379, 492, 607, 1162, 1165, 1199, 1235, 1351
Mox20I TGGCCA 1 cut(s) 240
Mph1103I ATGCAT 1 cut(s) 693
MscI TGGCCA 1 cut(s) 240
MseI TTAA 7 cut(s) 431, 686, 888, 1272, 1293, 1562, 1567
MslI CAYNNNNRTG 5 cut(s) 216, 468, 719, 736, 1103
Msp20I TGGCCA 1 cut(s) 240
MspI CCGG 2 cut(s) 216, 468
MspR9I CCNGG 4 cut(s) 153, 242, 468, 1013
MunI CAATTG 1 cut(s) 126
MvaI CCWGG 3 cut(s) 153, 242, 1013
MvnI CGCG 1 cut(s) 941
MwoI GCNNNNNNNGC 6 cut(s) 282, 1005, 1028, 1115, 1420, 1496
NciI CCSGG 1 cut(s) 468
NdeII GATC 2 cut(s) 330, 558
NlaIII CATG 2 cut(s) 1427, 1513
NlaIV GGNNCC 2 cut(s) 393, 621
NmuCI GTSAC 2 cut(s) 526, 1480
NsiI ATGCAT 1 cut(s) 693
NspI RCATGY 1 cut(s) 1427
OliI CACNNNNGTG 2 cut(s) 216, 468
PaqCI CACCTGC 1 cut(s) 1124
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 717
PfeI GAWTC 7 cut(s) 295, 634, 662, 898, 1112, 1306, 1550
PinAI ACCGGT 1 cut(s) 215
PkrI GCNGC 2 cut(s) 1205, 1247
PleI GAGTC 4 cut(s) 501, 619, 707, 1260
PmaCI CACGTG 3 cut(s) 259, 328, 556
PmlI CACGTG 3 cut(s) 259, 328, 556
PpsI GAGTC 4 cut(s) 501, 619, 707, 1260
Ppu21I YACGTR 3 cut(s) 259, 328, 556
PsiI TTATAA 1 cut(s) 1317
Psp6I CCWGG 3 cut(s) 151, 240, 1011
PspCI CACGTG 3 cut(s) 259, 328, 556
PspEI GGTNACC 1 cut(s) 526
PspGI CCWGG 3 cut(s) 151, 240, 1011
PspN4I GGNNCC 2 cut(s) 393, 621
RsaI GTAC 6 cut(s) 63, 195, 349, 374, 439, 602
RsaNI GTAC 6 cut(s) 62, 194, 348, 373, 438, 601
RseI CAYNNNNRTG 5 cut(s) 216, 468, 719, 736, 1103
SaqAI TTAA 7 cut(s) 431, 686, 888, 1272, 1293, 1562, 1567
SatI GCNGC 2 cut(s) 1204, 1246
Sau3AI GATC 2 cut(s) 330, 558
SchI GAGTC 4 cut(s) 501, 619, 708, 1260
ScrFI CCNGG 4 cut(s) 153, 242, 468, 1013
SduI GDGCHC 6 cut(s) 229, 248, 311, 523, 1141, 1416
SfcI CTRYAG 1 cut(s) 424
SgrAI CRCCGGYG 1 cut(s) 215
SmiMI CAYNNNNRTG 5 cut(s) 216, 468, 719, 736, 1103
SmlI CTYRAG 3 cut(s) 341, 509, 569
SmoI CTYRAG 3 cut(s) 341, 509, 569
Sse9I AATT 5 cut(s) 25, 126, 1034, 1088, 1456
SsiI CCGC 3 cut(s) 231, 1203, 1245
SspMI CTAG 5 cut(s) 362, 383, 611, 1095, 1376
StyD4I CCNGG 4 cut(s) 151, 240, 466, 1011
TaaI ACNGT 6 cut(s) 75, 105, 250, 442, 793, 916
TaiI ACGT 4 cut(s) 261, 330, 558, 1402
TaqI TCGA 2 cut(s) 183, 1553
TasI AATT 5 cut(s) 25, 126, 1034, 1088, 1456
TatI WGTACW 1 cut(s) 61
TauI GCSGC 2 cut(s) 1206, 1248
TfiI GAWTC 7 cut(s) 295, 634, 662, 898, 1112, 1306, 1550
Tru1I TTAA 7 cut(s) 431, 686, 888, 1272, 1293, 1562, 1567
Tru9I TTAA 7 cut(s) 431, 686, 888, 1272, 1293, 1562, 1567
TscAI CASTG 1 cut(s) 447
TseFI GTSAC 2 cut(s) 526, 1480
Tsp45I GTSAC 2 cut(s) 526, 1480
TspDTI ATGAA 3 cut(s) 659, 1526, 1563
TspRI CASTG 1 cut(s) 447
VneI GTGCAC 3 cut(s) 307, 519, 1137
XapI RAATTY 2 cut(s) 25, 1088
XceI RCATGY 1 cut(s) 1427
XspI CTAG 5 cut(s) 362, 383, 611, 1095, 1376
Zsp2I ATGCAT 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.