pycom05g08170

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000513
Physical Location & Seq
Forward (+)
10893756 .. 10894350
595 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g08170.4

Sequence Viewer

Length: 447 bp
ATGTTCACCTCTCGCACTTTACGCTCACCTTGGACTCAAGGTAGGTGCACAGGCTTGTCTCTTCACGTATCGATCGTAGCACTTGAGCGTATTTATTTACACCCAGTCCTGTCGTACAGACCACTAAAGGTGGCTCAGACTCGTGTGCAGGGATGTTTGATGAGCTATAGATTAAGTCGTACAGTGATGAGATATGGATACCTGCACCCCGGTGTTAGCGTTTCCGCTCGTGGCCAGGGCACAGTCCTTCACGTGATGTTCACCTCCCACACCTTACGCTCACCTTGGACTCAAGGTAGGTGCATAGTTTTCGCCCAGTCTTCGCTTGATCGTAGCACTTGGGCGTACTTATTTACACCCAGTCCTGTCGTACAGACCATTAGAGGTGGTTCCGACTCGTGTGCAGGGCTGCTTGATGAGATATGGATAAGTCGTACAGGTCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

149

Amino Acids

16.61

Weight (kDa)

10.58

Isoelectric Point (pI)

39.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 210
AccBSI CCGCTC 1 cut(s) 227
AciI CCGC 1 cut(s) 225
AcoI YGGCCR 1 cut(s) 232
AcvI CACGTG 1 cut(s) 253
AfaI GTAC 5 cut(s) 116, 181, 347, 372, 436
AjnI CCWGG 1 cut(s) 234
AleI CACNNNNGTG 1 cut(s) 210
AluBI AGCT 1 cut(s) 165
AluI AGCT 1 cut(s) 165
Alw21I GWGCWC 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 63
Alw44I GTGCAC 1 cut(s) 46
AoxI GGCC 1 cut(s) 232
ApaLI GTGCAC 1 cut(s) 46
ApeKI GCWGC 1 cut(s) 409
AsuC2I CCSGG 1 cut(s) 210
AsuHPI GGTGA 3 cut(s) 18, 253, 273
BaeGI GKGCMC 2 cut(s) 50, 242
BalI TGGCCA 1 cut(s) 234
BauI CACGAG 3 cut(s) 141, 228, 397
BbrPI CACGTG 1 cut(s) 253
BbsI GAAGAC 1 cut(s) 312
Bbv12I GWGCWC 1 cut(s) 50
BbvI GCAGC 1 cut(s) 396
BcgI CGANNNNNNTGC 4 cut(s) 292, 326, 383, 417
BciT130I CCWGG 1 cut(s) 236
BciVI GTATCC 1 cut(s) 191
BcnI CCSGG 1 cut(s) 210
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 1 cut(s) 445
BfmI CTRYAG 1 cut(s) 166
BfuAI ACCTGC 1 cut(s) 210
BfuI GTATCC 1 cut(s) 191
BisI GCNGC 1 cut(s) 410
BlsI GCNGC 1 cut(s) 411
Bme1390I CCNGG 2 cut(s) 210, 236
BmiI GGNNCC 1 cut(s) 391
BmrFI CCNGG 2 cut(s) 210, 236
BmrI ACTGGG 3 cut(s) 98, 310, 354
BmuI ACTGGG 3 cut(s) 98, 310, 354
BpiI GAAGAC 1 cut(s) 312
BpuEI CTTGAG 3 cut(s) 21, 104, 276
BpuMI CCSGG 1 cut(s) 210
Bsa29I ATCGAT 1 cut(s) 71
BsaAI YACGTR 2 cut(s) 67, 253
BsaJI CCNNGG 4 cut(s) 29, 208, 235, 284
Bse1I ACTGG 3 cut(s) 104, 316, 360
BseBI CCWGG 1 cut(s) 236
BseCI ATCGAT 1 cut(s) 71
BseDI CCNNGG 4 cut(s) 29, 208, 235, 284
BseGI GGATG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 3 cut(s) 104, 316, 360
BseSI GKGCMC 2 cut(s) 50, 242
BseXI GCAGC 1 cut(s) 396
BsgI GTGCAG 3 cut(s) 167, 188, 423
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 1 cut(s) 234
BshVI ATCGAT 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 75
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 1 cut(s) 210
BsmAI GTCTC 1 cut(s) 63
BsnI GGCC 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 50, 242
Bsp143I GATC 2 cut(s) 72, 328
BspACI CCGC 1 cut(s) 225
BspANI GGCC 1 cut(s) 234
BspCNI CTCAG 1 cut(s) 148
BspDI ATCGAT 1 cut(s) 71
BspLI GGNNCC 1 cut(s) 391
BspMI ACCTGC 1 cut(s) 210
BsrBI CCGCTC 1 cut(s) 227
BsrI ACTGG 3 cut(s) 104, 316, 360
BssECI CCNNGG 4 cut(s) 29, 208, 235, 284
BssMI GATC 2 cut(s) 72, 328
BssSI CACGAG 3 cut(s) 141, 228, 397
BssT1I CCWWGG 2 cut(s) 29, 284
Bst2BI CACGAG 3 cut(s) 141, 228, 397
Bst2UI CCWGG 1 cut(s) 236
Bst4CI ACNGT 2 cut(s) 184, 244
Bst6I CTCTTC 1 cut(s) 66
BstBAI YACGTR 2 cut(s) 67, 253
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 1 cut(s) 158
BstKTI GATC 2 cut(s) 75, 331
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 2 cut(s) 72, 328
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNI CCWGG 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 208, 234
BstSFI CTRYAG 1 cut(s) 166
BstSLI GKGCMC 2 cut(s) 50, 242
BstV1I GCAGC 1 cut(s) 396
BstV2I GAAGAC 1 cut(s) 312
Bsu15I ATCGAT 1 cut(s) 71
BsuI GTATCC 1 cut(s) 191
BsuRI GGCC 1 cut(s) 234
BsuTUI ATCGAT 1 cut(s) 71
BtsCI GGATG 1 cut(s) 158
BtsIMutI CAGTG 1 cut(s) 189
BveI ACCTGC 1 cut(s) 210
ClaI ATCGAT 1 cut(s) 71
Csp6I GTAC 5 cut(s) 115, 180, 346, 371, 435
CviJI RGCY 5 cut(s) 54, 134, 165, 234, 409
CviKI_1 RGCY 5 cut(s) 54, 134, 165, 234, 409
CviQI GTAC 5 cut(s) 115, 180, 346, 371, 435
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 74, 330
DpnII GATC 2 cut(s) 72, 328
EaeI YGGCCR 1 cut(s) 232
Eam1104I CTCTTC 1 cut(s) 66
EarI CTCTTC 1 cut(s) 66
Eco130I CCWWGG 2 cut(s) 29, 284
Eco72I CACGTG 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 234
EcoT14I CCWWGG 2 cut(s) 29, 284
ErhI CCWWGG 2 cut(s) 29, 284
FaiI YATR 4 cut(s) 168, 195, 305, 424
Fnu4HI GCNGC 1 cut(s) 410
FokI GGATG 1 cut(s) 165
Fsp4HI GCNGC 1 cut(s) 410
FspBI CTAG 1 cut(s) 445
GluI GCNGC 1 cut(s) 410
HaeIII GGCC 1 cut(s) 234
HapII CCGG 1 cut(s) 210
HinfI GANTC 4 cut(s) 34, 139, 289, 395
HpaII CCGG 1 cut(s) 210
HphI GGTGA 3 cut(s) 18, 253, 273
Hpy166II GTNNAC 3 cut(s) 6, 48, 261
Hpy188I TCNGA 2 cut(s) 138, 394
Hpy8I GTNNAC 3 cut(s) 6, 48, 261
HpyAV CCTTC 1 cut(s) 257
HpyCH4III ACNGT 2 cut(s) 184, 244
HpyCH4IV ACGT 2 cut(s) 66, 252
HpyCH4V TGCA 5 cut(s) 48, 148, 205, 303, 404
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 1 cut(s) 135
HpySE526I ACGT 2 cut(s) 66, 252
Kzo9I GATC 2 cut(s) 72, 328
Lsp1109I GCAGC 1 cut(s) 396
MaeI CTAG 1 cut(s) 445
MaeII ACGT 2 cut(s) 66, 252
MaeIII GTNAC 1 cut(s) 440
MalI GATC 2 cut(s) 74, 330
MbiI CCGCTC 1 cut(s) 227
MboI GATC 2 cut(s) 72, 328
MboII GAAGA 2 cut(s) 53, 312
MhlI GDGCHC 2 cut(s) 50, 242
MlsI TGGCCA 1 cut(s) 234
MluNI TGGCCA 1 cut(s) 234
MlyI GAGTC 4 cut(s) 28, 133, 283, 389
MmeI TCCRAC 1 cut(s) 417
MnlI CCTC 3 cut(s) 19, 274, 377
Mox20I TGGCCA 1 cut(s) 234
MscI TGGCCA 1 cut(s) 234
MseI TTAA 1 cut(s) 173
MslI CAYNNNNRTG 1 cut(s) 210
Msp20I TGGCCA 1 cut(s) 234
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 210, 236
MvaI CCWGG 1 cut(s) 236
MwoI GCNNNNNNNGC 1 cut(s) 21
NciI CCSGG 1 cut(s) 210
NdeII GATC 2 cut(s) 72, 328
NlaIV GGNNCC 1 cut(s) 391
NmuCI GTSAC 1 cut(s) 440
OliI CACNNNNGTG 1 cut(s) 210
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PkrI GCNGC 1 cut(s) 411
Ple19I CGATCG 1 cut(s) 75
PleI GAGTC 4 cut(s) 28, 133, 283, 389
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
PpsI GAGTC 4 cut(s) 28, 133, 283, 389
Ppu21I YACGTR 2 cut(s) 67, 253
Psp6I CCWGG 1 cut(s) 234
PspCI CACGTG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 234
PspN4I GGNNCC 1 cut(s) 391
PvuI CGATCG 1 cut(s) 75
RsaI GTAC 5 cut(s) 116, 181, 347, 372, 436
RsaNI GTAC 5 cut(s) 115, 180, 346, 371, 435
RseI CAYNNNNRTG 1 cut(s) 210
SaqAI TTAA 1 cut(s) 173
SatI GCNGC 1 cut(s) 410
Sau3AI GATC 2 cut(s) 72, 328
SchI GAGTC 4 cut(s) 28, 133, 283, 389
ScrFI CCNGG 2 cut(s) 210, 236
SduI GDGCHC 2 cut(s) 50, 242
SfcI CTRYAG 1 cut(s) 166
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 3 cut(s) 36, 83, 291
SmoI CTYRAG 3 cut(s) 36, 83, 291
SsiI CCGC 1 cut(s) 225
SspMI CTAG 1 cut(s) 445
StyD4I CCNGG 2 cut(s) 208, 234
StyI CCWWGG 2 cut(s) 29, 284
TaaI ACNGT 2 cut(s) 184, 244
TaiI ACGT 2 cut(s) 69, 255
TaqI TCGA 1 cut(s) 71
Tru1I TTAA 1 cut(s) 173
Tru9I TTAA 1 cut(s) 173
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 1 cut(s) 440
TseI GCWGC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 440
TspRI CASTG 1 cut(s) 189
VneI GTGCAC 1 cut(s) 46
XspI CTAG 1 cut(s) 445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.