Belongs to the peptidase A1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 900, 1014 |
| AccB1I | GGYRCC | 1 cut(s) | 1001 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 181 |
| AccII | CGCG | 3 cut(s) | 1180, 1182, 1334 |
| AciI | CCGC | 2 cut(s) | 259, 1178 |
| AclI | AACGTT | 2 cut(s) | 993, 1275 |
| AclWI | GGATC | 2 cut(s) | 1082, 1095 |
| AcoI | YGGCCR | 3 cut(s) | 778, 922, 1175 |
| AcsI | RAATTY | 3 cut(s) | 404, 414, 889 |
| AcvI | CACGTG | 2 cut(s) | 1173, 1197 |
| AcyI | GRCGYC | 4 cut(s) | 897, 1002, 1011, 1142 |
| AfaI | GTAC | 2 cut(s) | 641, 791 |
| AfeI | AGCGCT | 1 cut(s) | 51 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 181, 219, 739, 1184 |
| AflII | CTTAAG | 1 cut(s) | 301 |
| AgsI | TTSAA | 2 cut(s) | 106, 419 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 234 |
| AhlI | ACTAGT | 1 cut(s) | 953 |
| AjiI | CACGTC | 1 cut(s) | 764 |
| AjnI | CCWGG | 2 cut(s) | 144, 1165 |
| AluBI | AGCT | 5 cut(s) | 134, 529, 722, 836, 878 |
| AluI | AGCT | 5 cut(s) | 134, 529, 722, 836, 878 |
| Alw21I | GWGCWC | 2 cut(s) | 724, 1330 |
| Alw26I | GTCTC | 6 cut(s) | 200, 557, 587, 600, 709, 1330 |
| Alw44I | GTGCAC | 1 cut(s) | 1326 |
| AlwI | GGATC | 2 cut(s) | 1082, 1095 |
| Aor51HI | AGCGCT | 1 cut(s) | 51 |
| AoxI | GGCC | 4 cut(s) | 108, 778, 922, 1175 |
| ApaLI | GTGCAC | 1 cut(s) | 1326 |
| ApeKI | GCWGC | 1 cut(s) | 1191 |
| ApoI | RAATTY | 3 cut(s) | 404, 414, 889 |
| AseI | ATTAAT | 2 cut(s) | 69, 470 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 751 |
| AspLEI | GCGC | 3 cut(s) | 52, 1004, 1182 |
| AspS9I | GGNCC | 4 cut(s) | 86, 109, 189, 974 |
| AsuHPI | GGTGA | 2 cut(s) | 754, 1313 |
| AsuII | TTCGAA | 1 cut(s) | 915 |
| AvaII | GGWCC | 3 cut(s) | 86, 189, 974 |
| BaeGI | GKGCMC | 1 cut(s) | 1330 |
| BamHI | GGATCC | 1 cut(s) | 1087 |
| BanI | GGYRCC | 1 cut(s) | 1001 |
| BanII | GRGCYC | 1 cut(s) | 724 |
| BauI | CACGAG | 1 cut(s) | 704 |
| BbrPI | CACGTG | 2 cut(s) | 1173, 1197 |
| Bbv12I | GWGCWC | 2 cut(s) | 724, 1330 |
| BbvI | GCAGC | 1 cut(s) | 1203 |
| BccI | CCATC | 1 cut(s) | 127 |
| BceAI | ACGGC | 2 cut(s) | 1063, 1155 |
| BciT130I | CCWGG | 2 cut(s) | 146, 1167 |
| BclI | TGATCA | 1 cut(s) | 58 |
| BcoDI | GTCTC | 6 cut(s) | 200, 557, 587, 600, 709, 1330 |
| BcuI | ACTAGT | 1 cut(s) | 953 |
| BfaI | CTAG | 3 cut(s) | 954, 959, 1313 |
| BfoI | RGCGCY | 2 cut(s) | 53, 1005 |
| BfrI | CTTAAG | 1 cut(s) | 301 |
| BglII | AGATCT | 1 cut(s) | 339 |
| BisI | GCNGC | 2 cut(s) | 1178, 1192 |
| BlpI | GCTNAGC | 1 cut(s) | 723 |
| BlsI | GCNGC | 2 cut(s) | 1179, 1193 |
| BmcAI | AGTACT | 1 cut(s) | 791 |
| Bme1390I | CCNGG | 2 cut(s) | 146, 1167 |
| Bme18I | GGWCC | 3 cut(s) | 86, 189, 974 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 234 |
| BmgBI | CACGTC | 1 cut(s) | 764 |
| BmgT120I | GGNCC | 4 cut(s) | 86, 109, 189, 974 |
| BmiI | GGNNCC | 4 cut(s) | 318, 685, 1003, 1089 |
| BmrFI | CCNGG | 2 cut(s) | 146, 1167 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 253, 285, 706, 738 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 723 |
| Bpu14I | TTCGAA | 1 cut(s) | 915 |
| BsaAI | YACGTR | 2 cut(s) | 1173, 1197 |
| BsaBI | GATNNNNATC | 1 cut(s) | 338 |
| BsaHI | GRCGYC | 4 cut(s) | 897, 1002, 1011, 1142 |
| BsaI | GGTCTC | 1 cut(s) | 200 |
| BsaJI | CCNNGG | 1 cut(s) | 144 |
| BsaWI | WCCGGW | 2 cut(s) | 191, 1090 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 181, 219, 739, 1184 |
| Bse118I | RCCGGY | 3 cut(s) | 153, 1004, 1016 |
| Bse1I | ACTGG | 1 cut(s) | 1366 |
| Bse3DI | GCAATG | 1 cut(s) | 1232 |
| Bse8I | GATNNNNATC | 1 cut(s) | 338 |
| BseBI | CCWGG | 2 cut(s) | 146, 1167 |
| BseDI | CCNNGG | 1 cut(s) | 144 |
| BseJI | GATNNNNATC | 1 cut(s) | 338 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 181, 219, 739, 1184 |
| BseMI | GCAATG | 1 cut(s) | 1232 |
| BseMII | CTCAG | 1 cut(s) | 737 |
| BseNI | ACTGG | 1 cut(s) | 1366 |
| BseRI | GAGGAG | 1 cut(s) | 617 |
| BseSI | GKGCMC | 1 cut(s) | 1330 |
| BseXI | GCAGC | 1 cut(s) | 1203 |
| Bsh1236I | CGCG | 3 cut(s) | 1180, 1182, 1334 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1024 |
| BshFI | GGCC | 4 cut(s) | 110, 780, 924, 1177 |
| BshNI | GGYRCC | 1 cut(s) | 1001 |
| BsiEI | CGRYCG | 1 cut(s) | 1024 |
| BsiHKAI | GWGCWC | 2 cut(s) | 724, 1330 |
| BsiSI | CCGG | 6 cut(s) | 154, 192, 999, 1005, 1017, 1091 |
| BsiWI | CGTACG | 1 cut(s) | 639 |
| BslFI | GGGAC | 2 cut(s) | 374, 573 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 181, 219, 739, 1184 |
| BsmAI | GTCTC | 6 cut(s) | 200, 557, 587, 600, 709, 1330 |
| BsmBI | CGTCTC | 1 cut(s) | 709 |
| BsmFI | GGGAC | 2 cut(s) | 374, 573 |
| BsnI | GGCC | 4 cut(s) | 110, 780, 924, 1177 |
| Bso31I | GGTCTC | 1 cut(s) | 200 |
| Bsp119I | TTCGAA | 1 cut(s) | 915 |
| Bsp1286I | GDGCHC | 2 cut(s) | 724, 1330 |
| Bsp143I | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 723 |
| BspACI | CCGC | 2 cut(s) | 259, 1178 |
| BspANI | GGCC | 4 cut(s) | 110, 780, 924, 1177 |
| BspCNI | CTCAG | 1 cut(s) | 736 |
| BspFNI | CGCG | 3 cut(s) | 1180, 1182, 1334 |
| BspLI | GGNNCC | 4 cut(s) | 318, 685, 1003, 1089 |
| BspPI | GGATC | 2 cut(s) | 1082, 1095 |
| BspT104I | TTCGAA | 1 cut(s) | 915 |
| BspT107I | GGYRCC | 1 cut(s) | 1001 |
| BspTI | CTTAAG | 1 cut(s) | 301 |
| BspTNI | GGTCTC | 1 cut(s) | 200 |
| BsrDI | GCAATG | 1 cut(s) | 1232 |
| BsrFI | RCCGGY | 3 cut(s) | 153, 1004, 1016 |
| BsrI | ACTGG | 1 cut(s) | 1366 |
| BssAI | RCCGGY | 3 cut(s) | 153, 1004, 1016 |
| BssECI | CCNNGG | 1 cut(s) | 144 |
| BssMI | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| BssNI | GRCGYC | 4 cut(s) | 897, 1002, 1011, 1142 |
| BssSI | CACGAG | 1 cut(s) | 704 |
| Bst2BI | CACGAG | 1 cut(s) | 704 |
| Bst2UI | CCWGG | 2 cut(s) | 146, 1167 |
| Bst4CI | ACNGT | 3 cut(s) | 551, 794, 1156 |
| Bst6I | CTCTTC | 1 cut(s) | 82 |
| BstACI | GRCGYC | 4 cut(s) | 897, 1002, 1011, 1142 |
| BstAFI | CTTAAG | 1 cut(s) | 301 |
| BstBAI | YACGTR | 2 cut(s) | 1173, 1197 |
| BstBI | TTCGAA | 1 cut(s) | 915 |
| BstC8I | GCNNGC | 7 cut(s) | 155, 159, 370, 527, 1006, 1018, 1326 |
| BstDEI | CTNAG | 4 cut(s) | 343, 530, 723, 860 |
| BstEII | GGTNACC | 2 cut(s) | 12, 505 |
| BstFNI | CGCG | 3 cut(s) | 1180, 1182, 1334 |
| BstH2I | RGCGCY | 2 cut(s) | 53, 1005 |
| BstHHI | GCGC | 3 cut(s) | 52, 1004, 1182 |
| BstKTI | GATC | 6 cut(s) | 61, 336, 342, 678, 1024, 1090 |
| BstMAI | GTCTC | 6 cut(s) | 200, 557, 587, 600, 709, 1330 |
| BstMBI | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| BstMCI | CGRYCG | 1 cut(s) | 1024 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1188 |
| BstNI | CCWGG | 2 cut(s) | 146, 1167 |
| BstNSI | RCATGY | 1 cut(s) | 372 |
| BstPI | GGTNACC | 2 cut(s) | 12, 505 |
| BstSCI | CCNGG | 2 cut(s) | 144, 1165 |
| BstSLI | GKGCMC | 1 cut(s) | 1330 |
| BstUI | CGCG | 3 cut(s) | 1180, 1182, 1334 |
| BstV1I | GCAGC | 1 cut(s) | 1203 |
| BstX2I | RGATCY | 2 cut(s) | 339, 1087 |
| BstYI | RGATCY | 2 cut(s) | 339, 1087 |
| BsuRI | GGCC | 4 cut(s) | 110, 780, 924, 1177 |
| BtrI | CACGTC | 1 cut(s) | 764 |
| BtsIMutI | CAGTG | 2 cut(s) | 547, 1165 |
| Cac8I | GCNNGC | 7 cut(s) | 155, 159, 370, 527, 1006, 1018, 1326 |
| CfoI | GCGC | 3 cut(s) | 52, 1004, 1182 |
| Cfr10I | RCCGGY | 3 cut(s) | 153, 1004, 1016 |
| Cfr13I | GGNCC | 4 cut(s) | 86, 109, 189, 974 |
| CpoI | CGGWCCG | 1 cut(s) | 189 |
| CseI | GACGC | 2 cut(s) | 856, 1131 |
| Csp6I | GTAC | 2 cut(s) | 640, 790 |
| CspI | CGGWCCG | 1 cut(s) | 189 |
| CviAII | CATG | 2 cut(s) | 369, 798 |
| CviQI | GTAC | 2 cut(s) | 640, 790 |
| DdeI | CTNAG | 4 cut(s) | 343, 530, 723, 860 |
| DinI | GGCGCC | 1 cut(s) | 1003 |
| DpnI | GATC | 6 cut(s) | 60, 335, 341, 677, 1023, 1089 |
| DpnII | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| DraI | TTTAAA | 3 cut(s) | 409, 832, 1267 |
| DriI | GACNNNNNGTC | 1 cut(s) | 234 |
| EaeI | YGGCCR | 3 cut(s) | 778, 922, 1175 |
| Eam1104I | CTCTTC | 1 cut(s) | 82 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 234 |
| EarI | CTCTTC | 1 cut(s) | 82 |
| EciI | GGCGGA | 1 cut(s) | 274 |
| Ecl136II | GAGCTC | 1 cut(s) | 722 |
| Eco24I | GRGCYC | 1 cut(s) | 724 |
| Eco31I | GGTCTC | 1 cut(s) | 200 |
| Eco47I | GGWCC | 3 cut(s) | 86, 189, 974 |
| Eco47III | AGCGCT | 1 cut(s) | 51 |
| Eco53kI | GAGCTC | 1 cut(s) | 722 |
| Eco72I | CACGTG | 2 cut(s) | 1173, 1197 |
| Eco91I | GGTNACC | 2 cut(s) | 12, 505 |
| EcoICRI | GAGCTC | 1 cut(s) | 722 |
| EcoO109I | RGGNCCY | 1 cut(s) | 109 |
| EcoO65I | GGTNACC | 2 cut(s) | 12, 505 |
| EcoRII | CCWGG | 2 cut(s) | 144, 1165 |
| EcoT22I | ATGCAT | 2 cut(s) | 374, 382 |
| EcoT38I | GRGCYC | 1 cut(s) | 724 |
| EgeI | GGCGCC | 1 cut(s) | 1003 |
| EheI | GGCGCC | 1 cut(s) | 1003 |
| Esp3I | CGTCTC | 1 cut(s) | 709 |
| FaeI | CATG | 2 cut(s) | 372, 801 |
| FaqI | GGGAC | 2 cut(s) | 374, 573 |
| FatI | CATG | 2 cut(s) | 368, 797 |
| FbaI | TGATCA | 1 cut(s) | 58 |
| Fnu4HI | GCNGC | 2 cut(s) | 1178, 1192 |
| FriOI | GRGCYC | 1 cut(s) | 724 |
| Fsp4HI | GCNGC | 2 cut(s) | 1178, 1192 |
| FspBI | CTAG | 3 cut(s) | 954, 959, 1313 |
| GlaI | GCGC | 3 cut(s) | 51, 1003, 1181 |
| GluI | GCNGC | 2 cut(s) | 1178, 1192 |
| HaeII | RGCGCY | 2 cut(s) | 53, 1005 |
| HaeIII | GGCC | 4 cut(s) | 110, 780, 924, 1177 |
| HapII | CCGG | 6 cut(s) | 154, 192, 999, 1005, 1017, 1091 |
| HgaI | GACGC | 2 cut(s) | 856, 1131 |
| HhaI | GCGC | 3 cut(s) | 52, 1004, 1182 |
| Hin1I | GRCGYC | 4 cut(s) | 897, 1002, 1011, 1142 |
| Hin1II | CATG | 2 cut(s) | 372, 801 |
| Hin6I | GCGC | 3 cut(s) | 50, 1002, 1180 |
| HinP1I | GCGC | 3 cut(s) | 50, 1002, 1180 |
| HincII | GTYRAC | 3 cut(s) | 1083, 1150, 1273 |
| HindII | GTYRAC | 3 cut(s) | 1083, 1150, 1273 |
| HindIII | AAGCTT | 3 cut(s) | 132, 527, 834 |
| HinfI | GANTC | 2 cut(s) | 702, 981 |
| HpaI | GTTAAC | 2 cut(s) | 1083, 1273 |
| HpaII | CCGG | 6 cut(s) | 154, 192, 999, 1005, 1017, 1091 |
| HphI | GGTGA | 2 cut(s) | 754, 1313 |
| Hpy166II | GTNNAC | 4 cut(s) | 1083, 1150, 1273, 1328 |
| Hpy188I | TCNGA | 2 cut(s) | 872, 1060 |
| Hpy188III | TCNNGA | 7 cut(s) | 337, 706, 714, 851, 901, 985, 1135 |
| Hpy8I | GTNNAC | 4 cut(s) | 1083, 1150, 1273, 1328 |
| Hpy99I | CGWCG | 7 cut(s) | 640, 768, 872, 902, 1013, 1016, 1042 |
| HpyAV | CCTTC | 7 cut(s) | 100, 122, 642, 674, 898, 1027, 1365 |
| HpyCH4III | ACNGT | 3 cut(s) | 551, 794, 1156 |
| HpyCH4IV | ACGT | 8 cut(s) | 638, 763, 897, 993, 1011, 1172, 1196, 1275 |
| HpyCH4V | TGCA | 4 cut(s) | 372, 380, 801, 1328 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1188 |
| HpyF3I | CTNAG | 4 cut(s) | 343, 530, 723, 860 |
| HpySE526I | ACGT | 8 cut(s) | 638, 763, 897, 993, 1011, 1172, 1196, 1275 |
| Hsp92I | GRCGYC | 4 cut(s) | 897, 1002, 1011, 1142 |
| Hsp92II | CATG | 2 cut(s) | 372, 801 |
| HspAI | GCGC | 3 cut(s) | 50, 1002, 1180 |
| KasI | GGCGCC | 1 cut(s) | 1001 |
| KroI | GCCGGC | 3 cut(s) | 153, 1004, 1016 |
| KroNI | GCCGGC | 3 cut(s) | 155, 1006, 1018 |
| Ksp22I | TGATCA | 1 cut(s) | 58 |
| KspAI | GTTAAC | 2 cut(s) | 1083, 1273 |
| Kzo9I | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| LmnI | GCTCC | 1 cut(s) | 864 |
| Lsp1109I | GCAGC | 1 cut(s) | 1203 |
| MaeI | CTAG | 3 cut(s) | 954, 959, 1313 |
| MaeII | ACGT | 8 cut(s) | 638, 763, 897, 993, 1011, 1172, 1196, 1275 |
| MaeIII | GTNAC | 5 cut(s) | 12, 463, 505, 561, 1301 |
| MalI | GATC | 6 cut(s) | 60, 335, 341, 677, 1023, 1089 |
| MboI | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| MboII | GAAGA | 6 cut(s) | 69, 107, 608, 611, 614, 1042 |
| MflI | RGATCY | 2 cut(s) | 339, 1087 |
| MhlI | GDGCHC | 2 cut(s) | 724, 1330 |
| Mly113I | GGCGCC | 1 cut(s) | 1002 |
| MlyI | GAGTC | 1 cut(s) | 696 |
| MmeI | TCCRAC | 2 cut(s) | 850, 863 |
| MnlI | CCTC | 7 cut(s) | 91, 358, 581, 587, 638, 702, 1309 |
| Mph1103I | ATGCAT | 2 cut(s) | 374, 382 |
| MreI | CGCCGGCG | 2 cut(s) | 1004, 1016 |
| MroNI | GCCGGC | 3 cut(s) | 153, 1004, 1016 |
| MroXI | GAANNNNTTC | 1 cut(s) | 751 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1299, 1366 |
| MspCI | CTTAAG | 1 cut(s) | 301 |
| MspI | CCGG | 6 cut(s) | 154, 192, 999, 1005, 1017, 1091 |
| MspR9I | CCNGG | 2 cut(s) | 146, 1167 |
| MvaI | CCWGG | 2 cut(s) | 146, 1167 |
| MvnI | CGCG | 3 cut(s) | 1180, 1182, 1334 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1188 |
| NaeI | GCCGGC | 3 cut(s) | 155, 1006, 1018 |
| NarI | GGCGCC | 1 cut(s) | 1002 |
| NdeII | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| NgoMIV | GCCGGC | 3 cut(s) | 153, 1004, 1016 |
| NlaIII | CATG | 2 cut(s) | 372, 801 |
| NlaIV | GGNNCC | 4 cut(s) | 318, 685, 1003, 1089 |
| NmuCI | GTSAC | 2 cut(s) | 561, 1301 |
| NsiI | ATGCAT | 2 cut(s) | 374, 382 |
| NspI | RCATGY | 1 cut(s) | 372 |
| NspV | TTCGAA | 1 cut(s) | 915 |
| PaeI | GCATGC | 1 cut(s) | 372 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1017 |
| PdiI | GCCGGC | 3 cut(s) | 155, 1006, 1018 |
| PdmI | GAANNNNTTC | 1 cut(s) | 751 |
| PfeI | GAWTC | 1 cut(s) | 981 |
| Pfl23II | CGTACG | 1 cut(s) | 639 |
| PflFI | GACNNNGTC | 2 cut(s) | 1141, 1154 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 181 |
| PkrI | GCNGC | 2 cut(s) | 1179, 1193 |
| Ple19I | CGATCG | 1 cut(s) | 1024 |
| PleI | GAGTC | 1 cut(s) | 696 |
| PluTI | GGCGCC | 1 cut(s) | 1005 |
| PmaCI | CACGTG | 2 cut(s) | 1173, 1197 |
| PmlI | CACGTG | 2 cut(s) | 1173, 1197 |
| PpsI | GAGTC | 1 cut(s) | 696 |
| Ppu21I | YACGTR | 2 cut(s) | 1173, 1197 |
| PshBI | ATTAAT | 2 cut(s) | 69, 470 |
| Psp124BI | GAGCTC | 1 cut(s) | 724 |
| Psp1406I | AACGTT | 2 cut(s) | 993, 1275 |
| Psp6I | CCWGG | 2 cut(s) | 144, 1165 |
| PspCI | CACGTG | 2 cut(s) | 1173, 1197 |
| PspEI | GGTNACC | 2 cut(s) | 12, 505 |
| PspGI | CCWGG | 2 cut(s) | 144, 1165 |
| PspLI | CGTACG | 1 cut(s) | 639 |
| PspN4I | GGNNCC | 4 cut(s) | 318, 685, 1003, 1089 |
| PspPI | GGNCC | 4 cut(s) | 86, 109, 189, 974 |
| PsuI | RGATCY | 2 cut(s) | 339, 1087 |
| PsyI | GACNNNGTC | 2 cut(s) | 1141, 1154 |
| PvuI | CGATCG | 1 cut(s) | 1024 |
| RsaI | GTAC | 2 cut(s) | 641, 791 |
| RsaNI | GTAC | 2 cut(s) | 640, 790 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1299, 1366 |
| Rsr2I | CGGWCCG | 1 cut(s) | 189 |
| RsrII | CGGWCCG | 1 cut(s) | 189 |
| SacI | GAGCTC | 1 cut(s) | 724 |
| SatI | GCNGC | 2 cut(s) | 1178, 1192 |
| Sau3AI | GATC | 6 cut(s) | 58, 333, 339, 675, 1021, 1087 |
| Sau96I | GGNCC | 4 cut(s) | 86, 109, 189, 974 |
| ScaI | AGTACT | 1 cut(s) | 791 |
| SchI | GAGTC | 1 cut(s) | 696 |
| ScrFI | CCNGG | 2 cut(s) | 146, 1167 |
| SduI | GDGCHC | 2 cut(s) | 724, 1330 |
| SfoI | GGCGCC | 1 cut(s) | 1003 |
| SfuI | TTCGAA | 1 cut(s) | 915 |
| SgrAI | CRCCGGYG | 2 cut(s) | 1004, 1016 |
| SinI | GGWCC | 3 cut(s) | 86, 189, 974 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1299, 1366 |
| SmlI | CTYRAG | 1 cut(s) | 301 |
| SmoI | CTYRAG | 1 cut(s) | 301 |
| SpeI | ACTAGT | 1 cut(s) | 953 |
| SphI | GCATGC | 1 cut(s) | 372 |
| SsiI | CCGC | 2 cut(s) | 259, 1178 |
| SspDI | GGCGCC | 1 cut(s) | 1001 |
| SspI | AATATT | 6 cut(s) | 328, 426, 1046, 1068, 1110, 1131 |
| SspMI | CTAG | 3 cut(s) | 954, 959, 1313 |
| SstI | GAGCTC | 1 cut(s) | 724 |
| StyD4I | CCNGG | 2 cut(s) | 144, 1165 |
| TaaI | ACNGT | 3 cut(s) | 551, 794, 1156 |
| TaiI | ACGT | 8 cut(s) | 641, 766, 900, 996, 1014, 1175, 1199, 1278 |
| TaqI | TCGA | 8 cut(s) | 227, 336, 635, 678, 700, 713, 915, 1024 |
| TaqII | GACCGA | 2 cut(s) | 103, 177 |
| TatI | WGTACW | 1 cut(s) | 789 |
| TauI | GCSGC | 1 cut(s) | 1180 |
| TfiI | GAWTC | 1 cut(s) | 981 |
| TscAI | CASTG | 2 cut(s) | 554, 1165 |
| TseFI | GTSAC | 2 cut(s) | 561, 1301 |
| TseI | GCWGC | 1 cut(s) | 1191 |
| Tsp45I | GTSAC | 2 cut(s) | 561, 1301 |
| TspDTI | ATGAA | 6 cut(s) | 416, 760, 786, 957, 1043, 1197 |
| TspGWI | ACGGA | 8 cut(s) | 657, 1039, 1055, 1077, 1100, 1103, 1119, 1140 |
| TspRI | CASTG | 2 cut(s) | 554, 1165 |
| Tth111I | GACNNNGTC | 2 cut(s) | 1141, 1154 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 181 |
| Vha464I | CTTAAG | 1 cut(s) | 301 |
| VneI | GTGCAC | 1 cut(s) | 1326 |
| VpaK11BI | GGWCC | 3 cut(s) | 86, 189, 974 |
| VspI | ATTAAT | 2 cut(s) | 69, 470 |
| XapI | RAATTY | 3 cut(s) | 404, 414, 889 |
| XceI | RCATGY | 1 cut(s) | 372 |
| XmnI | GAANNNNTTC | 1 cut(s) | 751 |
| XspI | CTAG | 3 cut(s) | 954, 959, 1313 |
| ZraI | GACGTC | 2 cut(s) | 898, 1012 |
| ZrmI | AGTACT | 1 cut(s) | 791 |
| Zsp2I | ATGCAT | 2 cut(s) | 374, 382 |