pycom13g26020

plant mutator transposase zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000513
Physical Location & Seq
Forward (+)
22759644 .. 22761234
1591 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g26020.3

Sequence Viewer

Length: 1404 bp
ATGTGTAGCATCAATTTAATATTATTATTATTATTATTATTATTATTATTTTGTGTAGATCATGTCTTAGTTGTGCCTCTTTATCCACACTCTAGTTATGAGGATTTGTGTGGGCAATTAACTTCTAGGTTTTCAAGTTTATCATCAGGAGGTTTTCTGTTGAAATACTCATTATACGGAGGCAAACCAAATTGTTTATTAGAGTGCAATGAAGATATTCAGACACTTCTATTGGTGAATAATTTGATTTCAAATCCAATTGTTTATGTTTATATTCATGTTAAGAATGAGATGTCTATTATTTCTATTAGTAGTGTGTTGTCTTCTTGTCTAAGTAAAACTACTAGTAATGGATATGAGAACCAATATTTGGGTAAGTATGGCTCTTATGGACCTCACCATTATGCAACAAATGAGTGGCAGAGTTATATTTCTTATGAGGGTCAAAGTTATGAAGACGATGTAGTTGCGTTTTGTAGAAATGTACGCAATACTCCATTTGTAACATCCCTTGCCCCTAGTTCATTTCCGTCATGTTGTTTGGTTGTTTTGTGTGGTGTTGGCATGTGTTGGGCCACACACACACACACACACATTCTTCCTCTCTCTCTGCCTTCCTCCATGTCTCTCCCGTGTTCCCATTTCCCTTCCCTTTTCACTGTGTACGTACGGACTAACACACAACCACACCAAATCGTCACAGATAGAAGGAACAATTACATCTTTGAACTCGTGAGCCGTCGTTTTCACGTCGATTCGACGATGTCCGTTTTGAGCACTATTCATGCAAACATTAACCTAGCTCGTTTTTGGGATTTTGAAGCTCATAGATGTCTTAGTGTGGTCCCAAGGAGCATCGGAGTACTTCGTTGGACGAATTTGGACGTCGGGATAGCTTGGTTCGAAGTTGGCCGGTTTTTGGATTGTGTGGACAGGTATGATTGCGTGATTTTTAGCCCTTCAAACTTGTTTAGATGTGTTCTACTAGTCCTAGGCTTCAATTTGGTGCAAAAATCACGAAAAATGGTTGAAAAACGAGTGAGAAAACACGGTTCGCCGGAGAAGGTGACGGAATATTCCGTCAAGTCTGACGGAATATTCCTGACGCCGTTAACGGAATCTGTCAAAACTGACGGAATATTCCTAACGGCGTCAGTTGACGCCGTCAGCGTGCCAGGCACGTGCCTGCGCGTGGCCGGCGCGTGTGGCCTTGCCTTGGCCGGCAGCACGTTGGTATATTCATTTGTCCAAATTGAGCAATGTATGAGAAGTTATTACGAGAAGTTGGTTATGTGCTTTAAAGTTAACGTTTTTATAGTTATTTCGCATATAGGTGACACGTATCCTGAGGACGAGCGTGGTCACTCGAGGCAGGGGGGCTACGACCCTTCCACATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DBD_Tnp_Mut MULE SWIM

Protein Analysis

468

Amino Acids

52.42

Weight (kDa)

6.94

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 888
AccB7I CCANNNNNTGG 1 cut(s) 370
AccII CGCG 2 cut(s) 1191, 1202
AclI AACGTT 1 cut(s) 1308
AcoI YGGCCR 3 cut(s) 910, 1194, 1218
AcsI RAATTY 1 cut(s) 877
AcvI CACGTG 1 cut(s) 1182
AcyI GRCGYC 4 cut(s) 885, 1106, 1151, 1161
AfaI GTAC 4 cut(s) 486, 665, 669, 864
AfiI CCNNNNNNNGG 4 cut(s) 370, 919, 1192, 1216
AflIII ACRYGT 1 cut(s) 1338
AgsI TTSAA 8 cut(s) 135, 163, 252, 728, 821, 963, 1000, 1031
AhlI ACTAGT 2 cut(s) 344, 985
AjiI CACGTC 1 cut(s) 751
AjnI CCWGG 1 cut(s) 1174
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AluBI AGCT 3 cut(s) 803, 824, 896
AluI AGCT 3 cut(s) 803, 824, 896
Alw21I GWGCWC 1 cut(s) 779
Alw26I GTCTC 1 cut(s) 630
Ama87I CYCGRG 1 cut(s) 1366
AoxI GGCC 5 cut(s) 573, 910, 1194, 1207, 1218
ApeKI GCWGC 1 cut(s) 1224
ApoI RAATTY 1 cut(s) 877
Asp700I GAANNNNTTC 1 cut(s) 216
AspA2I CCTAGG 1 cut(s) 991
AspLEI GCGC 2 cut(s) 1191, 1202
AspS9I GGNCC 3 cut(s) 392, 573, 844
AsuHPI GGTGA 4 cut(s) 247, 389, 1078, 1346
AsuII TTCGAA 1 cut(s) 903
AvaI CYCGRG 1 cut(s) 1366
AvaII GGWCC 2 cut(s) 392, 844
AvrII CCTAGG 1 cut(s) 991
AxyI CCTNAGG 1 cut(s) 1347
BauI CACGAG 1 cut(s) 731
BbrPI CACGTG 1 cut(s) 1182
BbsI GAAGAC 2 cut(s) 315, 462
Bbv12I GWGCWC 1 cut(s) 779
BbvI GCAGC 1 cut(s) 1236
BceAI ACGGC 4 cut(s) 723, 1093, 1148, 1164
BcgI CGANNNNNNTGC 2 cut(s) 449, 483
BciT130I CCWGG 1 cut(s) 1176
BciVI GTATCC 1 cut(s) 1353
BcoDI GTCTC 1 cut(s) 630
BcuI ACTAGT 2 cut(s) 344, 985
BfaI CTAG 7 cut(s) 93, 126, 345, 519, 800, 986, 992
BfuI GTATCC 1 cut(s) 1353
BisI GCNGC 1 cut(s) 1225
BlnI CCTAGG 1 cut(s) 991
BlsI GCNGC 1 cut(s) 1226
BmcAI AGTACT 1 cut(s) 864
Bme1390I CCNGG 1 cut(s) 1176
Bme18I GGWCC 2 cut(s) 392, 844
BmeT110I CYCGRG 1 cut(s) 1366
BmgBI CACGTC 1 cut(s) 751
BmgT120I GGNCC 3 cut(s) 392, 573, 844
BmiI GGNNCC 1 cut(s) 846
BmrFI CCNGG 1 cut(s) 1176
BmsI GCATC 2 cut(s) 18, 864
BpiI GAAGAC 2 cut(s) 315, 462
Bpu14I TTCGAA 1 cut(s) 903
BsaAI YACGTR 3 cut(s) 667, 1182, 1341
BsaHI GRCGYC 4 cut(s) 885, 1106, 1151, 1161
BsaJI CCNNGG 3 cut(s) 848, 991, 1215
Bsc4I CCNNNNNNNGG 4 cut(s) 370, 919, 1192, 1216
Bse118I RCCGGY 3 cut(s) 912, 1196, 1220
Bse1I ACTGG 1 cut(s) 1399
Bse21I CCTNAGG 1 cut(s) 1347
Bse3DI GCAATG 2 cut(s) 214, 1265
BseBI CCWGG 1 cut(s) 1176
BseDI CCNNGG 3 cut(s) 848, 991, 1215
BseGI GGATG 1 cut(s) 506
BseLI CCNNNNNNNGG 4 cut(s) 370, 919, 1192, 1216
BseMI GCAATG 2 cut(s) 214, 1265
BseMII CTCAG 1 cut(s) 1338
BseNI ACTGG 1 cut(s) 1399
BseXI GCAGC 1 cut(s) 1236
Bsh1236I CGCG 2 cut(s) 1191, 1202
BshFI GGCC 5 cut(s) 575, 912, 1196, 1209, 1220
BsiHKAI GWGCWC 1 cut(s) 779
BsiHKCI CYCGRG 1 cut(s) 1366
BsiSI CCGG 4 cut(s) 913, 1058, 1197, 1221
BsiWI CGTACG 1 cut(s) 667
BslFI GGGAC 1 cut(s) 830
BslI CCNNNNNNNGG 4 cut(s) 370, 919, 1192, 1216
BsmAI GTCTC 1 cut(s) 630
BsmFI GGGAC 1 cut(s) 830
BsnI GGCC 5 cut(s) 575, 912, 1196, 1209, 1220
BsoBI CYCGRG 1 cut(s) 1366
Bsp119I TTCGAA 1 cut(s) 903
Bsp1286I GDGCHC 1 cut(s) 779
Bsp143I GATC 1 cut(s) 58
BspANI GGCC 5 cut(s) 575, 912, 1196, 1209, 1220
BspCNI CTCAG 1 cut(s) 1339
BspFNI CGCG 2 cut(s) 1191, 1202
BspLI GGNNCC 1 cut(s) 846
BspT104I TTCGAA 1 cut(s) 903
BsrDI GCAATG 2 cut(s) 214, 1265
BsrFI RCCGGY 3 cut(s) 912, 1196, 1220
BsrI ACTGG 1 cut(s) 1399
BssAI RCCGGY 3 cut(s) 912, 1196, 1220
BssECI CCNNGG 3 cut(s) 848, 991, 1215
BssMI GATC 1 cut(s) 58
BssNI GRCGYC 4 cut(s) 885, 1106, 1151, 1161
BssSI CACGAG 1 cut(s) 731
BssT1I CCWWGG 3 cut(s) 848, 991, 1215
Bst2BI CACGAG 1 cut(s) 731
Bst2UI CCWGG 1 cut(s) 1176
Bst4CI ACNGT 2 cut(s) 661, 1052
BstACI GRCGYC 4 cut(s) 885, 1106, 1151, 1161
BstBAI YACGTR 3 cut(s) 667, 1182, 1341
BstBI TTCGAA 1 cut(s) 903
BstC8I GCNNGC 4 cut(s) 1172, 1187, 1198, 1222
BstDEI CTNAG 4 cut(s) 67, 332, 836, 1347
BstF5I GGATG 1 cut(s) 506
BstFNI CGCG 2 cut(s) 1191, 1202
BstHHI GCGC 2 cut(s) 1191, 1202
BstKTI GATC 1 cut(s) 61
BstMAI GTCTC 1 cut(s) 630
BstMBI GATC 1 cut(s) 58
BstMWI GCNNNNNNNGC 3 cut(s) 1176, 1197, 1206
BstNI CCWGG 1 cut(s) 1176
BstNSI RCATGY 1 cut(s) 568
BstSCI CCNGG 1 cut(s) 1174
BstSNI TACGTA 1 cut(s) 667
BstUI CGCG 2 cut(s) 1191, 1202
BstV1I GCAGC 1 cut(s) 1236
BstV2I GAAGAC 2 cut(s) 315, 462
Bsu36I CCTNAGG 1 cut(s) 1347
BsuI GTATCC 1 cut(s) 1353
BsuRI GGCC 5 cut(s) 575, 912, 1196, 1209, 1220
BtrI CACGTC 1 cut(s) 751
BtsCI GGATG 1 cut(s) 506
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 4 cut(s) 1172, 1187, 1198, 1222
CfoI GCGC 2 cut(s) 1191, 1202
Cfr10I RCCGGY 3 cut(s) 912, 1196, 1220
Cfr13I GGNCC 3 cut(s) 392, 573, 844
CseI GACGC 3 cut(s) 1114, 1140, 1169
Csp6I GTAC 4 cut(s) 485, 664, 668, 863
CviAII CATG 6 cut(s) 62, 278, 534, 565, 622, 785
CviQI GTAC 4 cut(s) 485, 664, 668, 863
DdeI CTNAG 4 cut(s) 67, 332, 836, 1347
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
DraI TTTAAA 1 cut(s) 1300
EaeI YGGCCR 3 cut(s) 910, 1194, 1218
Eco105I TACGTA 1 cut(s) 667
Eco130I CCWWGG 3 cut(s) 848, 991, 1215
Eco47I GGWCC 2 cut(s) 392, 844
Eco72I CACGTG 1 cut(s) 1182
Eco81I CCTNAGG 1 cut(s) 1347
Eco88I CYCGRG 1 cut(s) 1366
EcoRII CCWGG 1 cut(s) 1174
EcoT14I CCWWGG 3 cut(s) 848, 991, 1215
ErhI CCWWGG 3 cut(s) 848, 991, 1215
FaeI CATG 6 cut(s) 65, 281, 537, 568, 625, 788
FaqI GGGAC 1 cut(s) 830
FatI CATG 6 cut(s) 61, 277, 533, 564, 621, 784
Fnu4HI GCNGC 1 cut(s) 1225
FokI GGATG 1 cut(s) 493
Fsp4HI GCNGC 1 cut(s) 1225
FspBI CTAG 7 cut(s) 93, 126, 345, 519, 800, 986, 992
GlaI GCGC 2 cut(s) 1190, 1201
GluI GCNGC 1 cut(s) 1225
HaeIII GGCC 5 cut(s) 575, 912, 1196, 1209, 1220
HapII CCGG 4 cut(s) 913, 1058, 1197, 1221
HgaI GACGC 3 cut(s) 1114, 1140, 1169
HhaI GCGC 2 cut(s) 1191, 1202
Hin1I GRCGYC 4 cut(s) 885, 1106, 1151, 1161
Hin1II CATG 6 cut(s) 65, 281, 537, 568, 625, 788
Hin6I GCGC 2 cut(s) 1189, 1200
HinP1I GCGC 2 cut(s) 1189, 1200
HincII GTYRAC 3 cut(s) 1113, 1159, 1306
HindII GTYRAC 3 cut(s) 1113, 1159, 1306
HinfI GANTC 2 cut(s) 755, 1118
HpaI GTTAAC 2 cut(s) 1113, 1306
HpaII CCGG 4 cut(s) 913, 1058, 1197, 1221
HphI GGTGA 4 cut(s) 247, 389, 1078, 1346
Hpy166II GTNNAC 5 cut(s) 664, 931, 1113, 1159, 1306
Hpy188I TCNGA 3 cut(s) 222, 860, 1090
Hpy188III TCNNGA 6 cut(s) 147, 733, 889, 1017, 1102, 1346
Hpy8I GTNNAC 5 cut(s) 664, 931, 1113, 1159, 1306
Hpy99I CGWCG 4 cut(s) 744, 755, 763, 890
HpyAV CCTTC 6 cut(s) 624, 657, 702, 969, 1057, 1398
HpyCH4III ACNGT 2 cut(s) 661, 1052
HpyCH4IV ACGT 7 cut(s) 666, 750, 885, 1181, 1229, 1308, 1340
HpyCH4V TGCA 4 cut(s) 207, 407, 788, 1009
HpyF10VI GCNNNNNNNGC 3 cut(s) 1176, 1197, 1206
HpyF3I CTNAG 4 cut(s) 67, 332, 836, 1347
HpySE526I ACGT 7 cut(s) 666, 750, 885, 1181, 1229, 1308, 1340
Hsp92I GRCGYC 4 cut(s) 885, 1106, 1151, 1161
Hsp92II CATG 6 cut(s) 65, 281, 537, 568, 625, 788
HspAI GCGC 2 cut(s) 1189, 1200
KroI GCCGGC 2 cut(s) 1196, 1220
KroNI GCCGGC 2 cut(s) 1198, 1222
KspAI GTTAAC 2 cut(s) 1113, 1306
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 1 cut(s) 852
Lsp1109I GCAGC 1 cut(s) 1236
LweI GCATC 2 cut(s) 18, 864
MaeI CTAG 7 cut(s) 93, 126, 345, 519, 800, 986, 992
MaeII ACGT 7 cut(s) 666, 750, 885, 1181, 1229, 1308, 1340
MaeIII GTNAC 5 cut(s) 502, 697, 1066, 1334, 1361
MalI GATC 1 cut(s) 60
MboI GATC 1 cut(s) 58
MboII GAAGA 4 cut(s) 224, 315, 467, 590
MfeI CAATTG 1 cut(s) 258
MhlI GDGCHC 1 cut(s) 779
MluCI AATT 9 cut(s) 13, 116, 190, 241, 258, 715, 877, 1000, 1251
MmeI TCCRAC 1 cut(s) 851
MroNI GCCGGC 2 cut(s) 1196, 1220
MroXI GAANNNNTTC 1 cut(s) 216
MseI TTAA 7 cut(s) 17, 119, 282, 795, 1112, 1299, 1305
MslI CAYNNNNRTG 3 cut(s) 402, 1332, 1399
MspI CCGG 4 cut(s) 913, 1058, 1197, 1221
MspR9I CCNGG 1 cut(s) 1176
MunI CAATTG 1 cut(s) 258
MvaI CCWGG 1 cut(s) 1176
MvnI CGCG 2 cut(s) 1191, 1202
MwoI GCNNNNNNNGC 3 cut(s) 1176, 1197, 1206
NaeI GCCGGC 2 cut(s) 1198, 1222
NdeII GATC 1 cut(s) 58
NgoMIV GCCGGC 2 cut(s) 1196, 1220
NlaIII CATG 6 cut(s) 65, 281, 537, 568, 625, 788
NlaIV GGNNCC 1 cut(s) 846
NmuCI GTSAC 4 cut(s) 697, 1066, 1334, 1361
NspI RCATGY 1 cut(s) 568
NspV TTCGAA 1 cut(s) 903
PaeR7I CTCGAG 1 cut(s) 1366
PcsI WCGNNNNNNNCGW 1 cut(s) 1167
PdiI GCCGGC 2 cut(s) 1198, 1222
PdmI GAANNNNTTC 1 cut(s) 216
PfeI GAWTC 2 cut(s) 755, 1118
Pfl23II CGTACG 1 cut(s) 667
PflFI GACNNNGTC 2 cut(s) 763, 1163
PflMI CCANNNNNTGG 1 cut(s) 370
PkrI GCNGC 1 cut(s) 1226
PmaCI CACGTG 1 cut(s) 1182
PmlI CACGTG 1 cut(s) 1182
Ppu21I YACGTR 3 cut(s) 667, 1182, 1341
Psp1406I AACGTT 1 cut(s) 1308
Psp6I CCWGG 1 cut(s) 1174
PspCI CACGTG 1 cut(s) 1182
PspGI CCWGG 1 cut(s) 1174
PspLI CGTACG 1 cut(s) 667
PspN4I GGNNCC 1 cut(s) 846
PspPI GGNCC 3 cut(s) 392, 573, 844
PspXI VCTCGAGB 1 cut(s) 1366
PsyI GACNNNGTC 2 cut(s) 763, 1163
RsaI GTAC 4 cut(s) 486, 665, 669, 864
RsaNI GTAC 4 cut(s) 485, 664, 668, 863
RseI CAYNNNNRTG 3 cut(s) 402, 1332, 1399
SaqAI TTAA 7 cut(s) 17, 119, 282, 795, 1112, 1299, 1305
SatI GCNGC 1 cut(s) 1225
Sau3AI GATC 1 cut(s) 58
Sau96I GGNCC 3 cut(s) 392, 573, 844
ScaI AGTACT 1 cut(s) 864
ScrFI CCNGG 1 cut(s) 1176
SduI GDGCHC 1 cut(s) 779
SfaNI GCATC 2 cut(s) 18, 864
Sfr274I CTCGAG 1 cut(s) 1366
SfuI TTCGAA 1 cut(s) 903
SinI GGWCC 2 cut(s) 392, 844
SlaI CTCGAG 1 cut(s) 1366
SmiMI CAYNNNNRTG 3 cut(s) 402, 1332, 1399
SmlI CTYRAG 1 cut(s) 1366
SmoI CTYRAG 1 cut(s) 1366
SnaBI TACGTA 1 cut(s) 667
SpeI ACTAGT 2 cut(s) 344, 985
Sse9I AATT 9 cut(s) 13, 116, 190, 241, 258, 715, 877, 1000, 1251
SspI AATATT 5 cut(s) 21, 368, 1076, 1098, 1140
SspMI CTAG 7 cut(s) 93, 126, 345, 519, 800, 986, 992
StyD4I CCNGG 1 cut(s) 1174
StyI CCWWGG 3 cut(s) 848, 991, 1215
TaaI ACNGT 2 cut(s) 661, 1052
TaiI ACGT 7 cut(s) 669, 753, 888, 1184, 1232, 1311, 1343
TaqI TCGA 4 cut(s) 753, 758, 903, 1367
TasI AATT 9 cut(s) 13, 116, 190, 241, 258, 715, 877, 1000, 1251
TatI WGTACW 1 cut(s) 862
TfiI GAWTC 2 cut(s) 755, 1118
Tru1I TTAA 7 cut(s) 17, 119, 282, 795, 1112, 1299, 1305
Tru9I TTAA 7 cut(s) 17, 119, 282, 795, 1112, 1299, 1305
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 4 cut(s) 697, 1066, 1334, 1361
TseI GCWGC 1 cut(s) 1224
Tsp45I GTSAC 4 cut(s) 697, 1066, 1334, 1361
TspDTI ATGAA 6 cut(s) 225, 266, 468, 513, 773, 1230
TspGWI ACGGA 9 cut(s) 192, 519, 685, 757, 1069, 1085, 1107, 1130, 1149
TspRI CASTG 1 cut(s) 664
Tth111I GACNNNGTC 2 cut(s) 763, 1163
Van91I CCANNNNNTGG 1 cut(s) 370
VpaK11BI GGWCC 2 cut(s) 392, 844
XapI RAATTY 1 cut(s) 877
XceI RCATGY 1 cut(s) 568
XhoI CTCGAG 1 cut(s) 1366
XmaJI CCTAGG 1 cut(s) 991
XmnI GAANNNNTTC 1 cut(s) 216
XspI CTAG 7 cut(s) 93, 126, 345, 519, 800, 986, 992
ZraI GACGTC 1 cut(s) 886
ZrmI AGTACT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.