pycom520g00880

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000513
Physical Location & Seq
Forward (+)
674569 .. 677185
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00880.13

Sequence Viewer

Length: 1779 bp
ATGCACTCTTGTAGATGTGTTTGTGAGGTCCCAAGGAGCCTCGGAGTAGTTCGTTGGACGAATTTGGACGTTGGGAAGGCTAGGTTCAAAGTTGGCCGAACTTTGATTGTTGTTGCAGCTTCATTTTGGTATAAAGAACGTGAAATTTGGTTGAAAAACGAAGGAGAAAACAAAGTTGGAAAATTTCCCAGTTTTCCGGCGGCGGCGCCGGCGACGGCGGCGGCGCCGGCGACGGCGACTGGCCGGAGAAGACAGGAGAATATTCCGTTAATTAAGGTTAACGGAATATTCCGTCAGTGTGCAGTGCACGTGGGCCGCGCGTGGGGGCGCGTAGGGCCGTGCCTGTGGCGGCGCGTGGGGGCGCGTGCGTGGTCCAAAAATTTTTCTAAAAATTTGTGTGTGATCCTGAGGTTGTGTAGATCACGTTGGTATATTCAATTGTCCAATTTGAGCAATGTTTTCGTAACTTTGTCGCGTATAGGTGATTCGTTTTCCGAAGACGAGCCAGTGAGTGGGCTTTTGTTTTCCGTATATACCTATATACAGTTATATTCCCAGAAATTTAATAGAAAATGTTATTTGTTTTATGCCATGCATTATATGAATGTTGTTTACGCATCATTGCATGTATTATTGGTGATATACATACATATATACATGTGTATTTGGTGCGGTGGATGCACAGGTAAGTGCCAGGTAAGCGGTATTCAGATGCTTAGAGAGCTCATAATCTGCACCCCCGGTGTTAGTGCTCCCGTCCAGGGCCAGGGCACAGCCTTCACGTGTATGTTCACCAGCACCGCATGCTCGTCTTGGATCCAAGTTAGGTGCAAGCCTGTCGTAGAGATCACATTAGGTGGTTCCGACTTCACTAGAGCATACATATATATTACACCCAGCTTGTCGTGCAGACCACTTGAGCGTGGAGCTCTAGGTGCAGCGGTACATGTCACGTTAGGTGACTCCCGGCTGCCAGTTTATATGTTATTCTGTCATGGCATATTCTCGAGCATAAATGGCATATCATGGAGCATGATTGACATACCTATAAATACGTATATATGTTCATTTTCTGGGAAAGTTTTCAAAGAACTTTGTTTTTGCCCACTCACGCTTTTGTTTTTCGCCCCTCCAGGTTCTAGTGGTCTAGCAAGTTCGGTGGTATATCCCAGAGGGCGTCCCGGCATTTCTGACAGACATTCACCAACTTGCTCTGAAGTGTGTCTCACGTACACTAGTATTTGTATTAGTACTTTGTATGCTATTAGGTTTTTAATTATTCGTACTTTTTTTTATTACTATCTTAATAGCTTCCGCACGCGCACATGGTTACGTCACCTTCGTAAATACCTGGTGGTGGTGGTTGACAAGGTCTACAAGGACAATAATATATGTTATTTATACAAAATAAACAAGTATGATATCAGTTGTATCAATGACTTCCATCCTCAAGTCGCACAGCAGGCCCGGTTAAATAGTCGAATGACCATTATAACAACTAGACATAAAACCTTGCACATGGAACTCGACATGTTTCAGCATGACGGCCACTCACAGTATAAGTACAAGTTATTTGTATGGCAAGCATTCAACTATAACAGAGTGCTCGAACAAAGTATGATCGTCATAAACAAACACACTAAAGAAAGAAAAGAAAACTTACTTAAGACTTGGAGATTGCTTGAATATATAGTTGTTTACAAGAAAGTATGGTGTGAGTATATGGTGAGAGTAAGGTGTTCAGGTGTTGAGATGCTGAGGTATATTTACAATGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

593

Amino Acids

68.15

Weight (kDa)

9.54

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1493
AccB1I GGYRCC 2 cut(s) 205, 223
AccB7I CCANNNNNTGG 1 cut(s) 512
AccI GTMKAC 1 cut(s) 1374
AccII CGCG 7 cut(s) 318, 320, 330, 354, 364, 475, 1321
AclWI GGATC 3 cut(s) 397, 811, 824
AcoI YGGCCR 3 cut(s) 94, 241, 1546
AcsI RAATTY 6 cut(s) 61, 144, 182, 379, 391, 560
AcuI CTGAAG 1 cut(s) 1236
AcvI CACGTG 2 cut(s) 310, 783
AcyI GRCGYC 3 cut(s) 206, 224, 1177
AfaI GTAC 5 cut(s) 945, 1232, 1252, 1285, 1565
AfiI CCNNNNNNNGG 5 cut(s) 322, 512, 761, 766, 1357
AflII CTTAAG 1 cut(s) 1664
AflIII ACRYGT 4 cut(s) 657, 782, 946, 1530
AgsI TTSAA 6 cut(s) 88, 154, 437, 1087, 1591, 1685
AhlI ACTAGT 1 cut(s) 1235
AjnI CCWGG 5 cut(s) 693, 759, 765, 1132, 1350
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 5 cut(s) 119, 724, 900, 929, 1311
AluI AGCT 5 cut(s) 119, 724, 900, 929, 1311
Alw21I GWGCWC 5 cut(s) 309, 726, 754, 931, 1608
Alw26I GTCTC 1 cut(s) 1229
Alw44I GTGCAC 1 cut(s) 305
AlwI GGATC 3 cut(s) 397, 811, 824
Ama87I CYCGRG 1 cut(s) 1006
AoxI GGCC 7 cut(s) 94, 241, 313, 335, 763, 1464, 1546
ApaLI GTGCAC 1 cut(s) 305
ApeKI GCWGC 3 cut(s) 116, 938, 970
ApoI RAATTY 6 cut(s) 61, 144, 182, 379, 391, 560
Asp700I GAANNNNTTC 1 cut(s) 1082
AspLEI GCGC 7 cut(s) 208, 226, 320, 330, 354, 364, 1323
AspS9I GGNCC 6 cut(s) 28, 313, 335, 372, 763, 1465
AsuC2I CCSGG 4 cut(s) 741, 967, 1182, 1468
AsuHPI GGTGA 7 cut(s) 494, 649, 784, 971, 1194, 1328, 1738
AvaI CYCGRG 1 cut(s) 1006
AvaII GGWCC 2 cut(s) 28, 372
AxyI CCTNAGG 1 cut(s) 407
BaeGI GKGCMC 2 cut(s) 309, 773
BamHI GGATCC 1 cut(s) 816
BanI GGYRCC 2 cut(s) 205, 223
BanII GRGCYC 2 cut(s) 726, 931
BbrPI CACGTG 2 cut(s) 310, 783
BbsI GAAGAC 2 cut(s) 256, 504
Bbv12I GWGCWC 5 cut(s) 309, 726, 754, 931, 1608
BbvCI CCTCAGC 1 cut(s) 1757
BbvI GCAGC 3 cut(s) 128, 950, 957
BccI CCATC 1 cut(s) 1452
BceAI ACGGC 4 cut(s) 231, 249, 322, 1561
BcgI CGANNNNNNTGC 4 cut(s) 442, 476, 820, 854
BciT130I CCWGG 5 cut(s) 695, 761, 767, 1134, 1352
BcnI CCSGG 4 cut(s) 741, 967, 1182, 1468
BcoDI GTCTC 1 cut(s) 1229
BcuI ACTAGT 1 cut(s) 1235
BfaI CTAG 7 cut(s) 81, 873, 932, 1140, 1148, 1236, 1500
BfoI RGCGCY 2 cut(s) 209, 227
BfrI CTTAAG 1 cut(s) 1664
BisI GCNGC 9 cut(s) 117, 201, 204, 219, 222, 316, 350, 939, 971
BlsI GCNGC 9 cut(s) 118, 202, 205, 220, 223, 317, 351, 940, 972
BmcAI AGTACT 1 cut(s) 1252
Bme1390I CCNGG 9 cut(s) 695, 741, 761, 767, 967, 1134, 1182, 1352, 1468
Bme18I GGWCC 2 cut(s) 28, 372
BmeT110I CYCGRG 1 cut(s) 1006
BmgT120I GGNCC 6 cut(s) 28, 313, 335, 372, 763, 1465
BmiI GGNNCC 6 cut(s) 30, 38, 207, 225, 818, 862
BmrFI CCNGG 9 cut(s) 695, 741, 761, 767, 967, 1134, 1182, 1352, 1468
BmrI ACTGGG 1 cut(s) 183
BmsI GCATC 4 cut(s) 626, 668, 702, 1743
BmuI ACTGGG 1 cut(s) 183
BpiI GAAGAC 2 cut(s) 256, 504
BpmI CTGGAG 1 cut(s) 1116
Bpu10I CCTNAGC 1 cut(s) 1757
BpuEI CTTGAG 2 cut(s) 938, 1434
BpuMI CCSGG 4 cut(s) 741, 967, 1182, 1468
BsaAI YACGTR 4 cut(s) 310, 783, 1056, 1230
BsaHI GRCGYC 3 cut(s) 206, 224, 1177
BsaJI CCNNGG 5 cut(s) 32, 40, 739, 760, 766
Bsc4I CCNNNNNNNGG 5 cut(s) 322, 512, 761, 766, 1357
Bse118I RCCGGY 2 cut(s) 208, 226
Bse1I ACTGG 4 cut(s) 189, 244, 506, 974
Bse21I CCTNAGG 1 cut(s) 407
Bse3DI GCAATG 2 cut(s) 460, 620
BseBI CCWGG 5 cut(s) 695, 761, 767, 1134, 1352
BseDI CCNNGG 5 cut(s) 32, 40, 739, 760, 766
BseGI GGATG 2 cut(s) 683, 1444
BseLI CCNNNNNNNGG 5 cut(s) 322, 512, 761, 766, 1357
BseMI GCAATG 2 cut(s) 460, 620
BseMII CTCAG 2 cut(s) 398, 1748
BseNI ACTGG 4 cut(s) 189, 244, 506, 974
BseSI GKGCMC 2 cut(s) 309, 773
BseXI GCAGC 3 cut(s) 128, 950, 957
BseYI CCCAGC 1 cut(s) 896
BsgI GTGCAG 4 cut(s) 321, 718, 928, 957
Bsh1236I CGCG 7 cut(s) 318, 320, 330, 354, 364, 475, 1321
BshFI GGCC 7 cut(s) 96, 243, 315, 337, 765, 1466, 1548
BshNI GGYRCC 2 cut(s) 205, 223
BsiHKAI GWGCWC 5 cut(s) 309, 726, 754, 931, 1608
BsiHKCI CYCGRG 1 cut(s) 1006
BsiSI CCGG 8 cut(s) 197, 209, 227, 244, 741, 967, 1182, 1468
BslFI GGGAC 2 cut(s) 14, 1164
BslI CCNNNNNNNGG 5 cut(s) 322, 512, 761, 766, 1357
BsmAI GTCTC 1 cut(s) 1229
BsmFI GGGAC 2 cut(s) 14, 1164
BsmI GAATGC 1 cut(s) 1586
BsnI GGCC 7 cut(s) 96, 243, 315, 337, 765, 1466, 1548
BsoBI CYCGRG 1 cut(s) 1006
Bsp1286I GDGCHC 6 cut(s) 309, 726, 754, 773, 931, 1608
Bsp143I GATC 5 cut(s) 402, 419, 816, 846, 1620
BspANI GGCC 7 cut(s) 96, 243, 315, 337, 765, 1466, 1548
BspCNI CTCAG 2 cut(s) 399, 1749
BspFNI CGCG 7 cut(s) 318, 320, 330, 354, 364, 475, 1321
BspLI GGNNCC 6 cut(s) 30, 38, 207, 225, 818, 862
BspPI GGATC 3 cut(s) 397, 811, 824
BspT107I GGYRCC 2 cut(s) 205, 223
BspTI CTTAAG 1 cut(s) 1664
BsrDI GCAATG 2 cut(s) 460, 620
BsrFI RCCGGY 2 cut(s) 208, 226
BsrI ACTGG 4 cut(s) 189, 244, 506, 974
BssAI RCCGGY 2 cut(s) 208, 226
BssECI CCNNGG 5 cut(s) 32, 40, 739, 760, 766
BssMI GATC 5 cut(s) 402, 419, 816, 846, 1620
BssNI GRCGYC 3 cut(s) 206, 224, 1177
BssT1I CCWWGG 1 cut(s) 32
Bst2UI CCWGG 5 cut(s) 695, 761, 767, 1134, 1352
Bst4CI ACNGT 2 cut(s) 546, 1557
BstACI GRCGYC 3 cut(s) 206, 224, 1177
BstAFI CTTAAG 1 cut(s) 1664
BstAPI GCANNNNNTGC 1 cut(s) 804
BstBAI YACGTR 4 cut(s) 310, 783, 1056, 1230
BstC8I GCNNGC 8 cut(s) 210, 228, 366, 805, 833, 1319, 1464, 1584
BstDEI CTNAG 3 cut(s) 407, 716, 1757
BstF5I GGATG 2 cut(s) 683, 1444
BstFNI CGCG 7 cut(s) 318, 320, 330, 354, 364, 475, 1321
BstH2I RGCGCY 2 cut(s) 209, 227
BstHHI GCGC 7 cut(s) 208, 226, 320, 330, 354, 364, 1323
BstKTI GATC 5 cut(s) 405, 422, 819, 849, 1623
BstMAI GTCTC 1 cut(s) 1229
BstMBI GATC 5 cut(s) 402, 419, 816, 846, 1620
BstNI CCWGG 5 cut(s) 695, 761, 767, 1134, 1352
BstNSI RCATGY 5 cut(s) 629, 661, 807, 950, 1534
BstSCI CCNGG 9 cut(s) 693, 739, 759, 765, 965, 1132, 1180, 1350, 1466
BstSLI GKGCMC 2 cut(s) 309, 773
BstSNI TACGTA 1 cut(s) 1056
BstUI CGCG 7 cut(s) 318, 320, 330, 354, 364, 475, 1321
BstV1I GCAGC 3 cut(s) 128, 950, 957
BstV2I GAAGAC 2 cut(s) 256, 504
BstX2I RGATCY 1 cut(s) 816
BstYI RGATCY 1 cut(s) 816
Bsu36I CCTNAGG 1 cut(s) 407
BsuRI GGCC 7 cut(s) 96, 243, 315, 337, 765, 1466, 1548
BtsCI GGATG 2 cut(s) 683, 1444
BtsI GCAGTG 1 cut(s) 309
BtsIMutI CAGTG 3 cut(s) 302, 309, 513
Cac8I GCNNGC 8 cut(s) 210, 228, 366, 805, 833, 1319, 1464, 1584
CfoI GCGC 7 cut(s) 208, 226, 320, 330, 354, 364, 1323
Cfr10I RCCGGY 2 cut(s) 208, 226
Cfr13I GGNCC 6 cut(s) 28, 313, 335, 372, 763, 1465
CseI GACGC 1 cut(s) 1166
CsiI ACCWGGT 1 cut(s) 1350
Csp6I GTAC 5 cut(s) 944, 1231, 1251, 1284, 1564
CspCI CAANNNNNGTGG 2 cut(s) 1140, 1175
CviQI GTAC 5 cut(s) 944, 1231, 1251, 1284, 1564
DdeI CTNAG 3 cut(s) 407, 716, 1757
DinI GGCGCC 2 cut(s) 207, 225
DpnI GATC 5 cut(s) 404, 421, 818, 848, 1622
DpnII GATC 5 cut(s) 402, 419, 816, 846, 1620
EaeI YGGCCR 3 cut(s) 94, 241, 1546
Ecl136II GAGCTC 2 cut(s) 724, 929
Eco105I TACGTA 1 cut(s) 1056
Eco130I CCWWGG 1 cut(s) 32
Eco24I GRGCYC 2 cut(s) 726, 931
Eco32I GATATC 1 cut(s) 1423
Eco47I GGWCC 2 cut(s) 28, 372
Eco53kI GAGCTC 2 cut(s) 724, 929
Eco57I CTGAAG 1 cut(s) 1236
Eco72I CACGTG 2 cut(s) 310, 783
Eco81I CCTNAGG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 1006
EcoICRI GAGCTC 2 cut(s) 724, 929
EcoO109I RGGNCCY 1 cut(s) 28
EcoRII CCWGG 5 cut(s) 693, 759, 765, 1132, 1350
EcoRV GATATC 1 cut(s) 1423
EcoT14I CCWWGG 1 cut(s) 32
EcoT22I ATGCAT 1 cut(s) 597
EcoT38I GRGCYC 2 cut(s) 726, 931
EgeI GGCGCC 2 cut(s) 207, 225
EheI GGCGCC 2 cut(s) 207, 225
ErhI CCWWGG 1 cut(s) 32
FaqI GGGAC 2 cut(s) 14, 1164
FblI GTMKAC 1 cut(s) 1374
Fnu4HI GCNGC 9 cut(s) 117, 201, 204, 219, 222, 316, 350, 939, 971
FokI GGATG 2 cut(s) 690, 1431
FriOI GRGCYC 2 cut(s) 726, 931
Fsp4HI GCNGC 9 cut(s) 117, 201, 204, 219, 222, 316, 350, 939, 971
FspBI CTAG 7 cut(s) 81, 873, 932, 1140, 1148, 1236, 1500
GlaI GCGC 7 cut(s) 207, 225, 319, 329, 353, 363, 1322
GluI GCNGC 9 cut(s) 117, 201, 204, 219, 222, 316, 350, 939, 971
GsaI CCCAGC 1 cut(s) 900
GsuI CTGGAG 1 cut(s) 1116
HaeII RGCGCY 2 cut(s) 209, 227
HaeIII GGCC 7 cut(s) 96, 243, 315, 337, 765, 1466, 1548
HapII CCGG 8 cut(s) 197, 209, 227, 244, 741, 967, 1182, 1468
HgaI GACGC 1 cut(s) 1166
HhaI GCGC 7 cut(s) 208, 226, 320, 330, 354, 364, 1323
Hin1I GRCGYC 3 cut(s) 206, 224, 1177
Hin6I GCGC 7 cut(s) 206, 224, 318, 328, 352, 362, 1321
HinP1I GCGC 7 cut(s) 206, 224, 318, 328, 352, 362, 1321
HincII GTYRAC 2 cut(s) 280, 1366
HindII GTYRAC 2 cut(s) 280, 1366
HinfI GANTC 2 cut(s) 485, 962
HpaI GTTAAC 1 cut(s) 280
HpaII CCGG 8 cut(s) 197, 209, 227, 244, 741, 967, 1182, 1468
HphI GGTGA 7 cut(s) 494, 649, 784, 971, 1194, 1328, 1738
Hpy166II GTNNAC 8 cut(s) 280, 307, 613, 792, 1233, 1366, 1375, 1699
Hpy188I TCNGA 6 cut(s) 44, 496, 711, 865, 1192, 1216
Hpy188III TCNNGA 2 cut(s) 406, 1006
Hpy8I GTNNAC 8 cut(s) 280, 307, 613, 792, 1233, 1366, 1375, 1699
Hpy99I CGWCG 2 cut(s) 217, 235
HpyAV CCTTC 4 cut(s) 70, 155, 787, 1349
HpyCH4III ACNGT 2 cut(s) 546, 1557
HpyCH4IV ACGT 9 cut(s) 69, 139, 309, 424, 782, 953, 1055, 1229, 1333
HpyF3I CTNAG 3 cut(s) 407, 716, 1757
HpySE526I ACGT 9 cut(s) 69, 139, 309, 424, 782, 953, 1055, 1229, 1333
Hsp92I GRCGYC 3 cut(s) 206, 224, 1177
HspAI GCGC 7 cut(s) 206, 224, 318, 328, 352, 362, 1321
KasI GGCGCC 2 cut(s) 205, 223
KroI GCCGGC 2 cut(s) 208, 226
KroNI GCCGGC 2 cut(s) 210, 228
KspAI GTTAAC 1 cut(s) 280
Kzo9I GATC 5 cut(s) 402, 419, 816, 846, 1620
LmnI GCTCC 4 cut(s) 36, 757, 926, 1029
Lsp1109I GCAGC 3 cut(s) 128, 950, 957
LweI GCATC 4 cut(s) 626, 668, 702, 1743
MabI ACCWGGT 1 cut(s) 1350
MaeI CTAG 7 cut(s) 81, 873, 932, 1140, 1148, 1236, 1500
MaeII ACGT 9 cut(s) 69, 139, 309, 424, 782, 953, 1055, 1229, 1333
MaeIII GTNAC 5 cut(s) 463, 949, 959, 1329, 1334
MalI GATC 5 cut(s) 404, 421, 818, 848, 1622
MboI GATC 5 cut(s) 402, 419, 816, 846, 1620
MboII GAAGA 2 cut(s) 261, 509
MfeI CAATTG 1 cut(s) 437
MflI RGATCY 1 cut(s) 816
MhlI GDGCHC 6 cut(s) 309, 726, 754, 773, 931, 1608
Mly113I GGCGCC 2 cut(s) 206, 224
MlyI GAGTC 1 cut(s) 956
MmeI TCCRAC 3 cut(s) 35, 157, 888
MnlI CCTC 7 cut(s) 19, 50, 402, 1140, 1166, 1458, 1752
Mph1103I ATGCAT 1 cut(s) 597
MreI CGCCGGCG 2 cut(s) 208, 226
MroNI GCCGGC 2 cut(s) 208, 226
MroXI GAANNNNTTC 1 cut(s) 1082
MseI TTAA 8 cut(s) 269, 273, 279, 564, 1274, 1305, 1472, 1665
MslI CAYNNNNRTG 1 cut(s) 785
MspA1I CMGCKG 1 cut(s) 941
MspCI CTTAAG 1 cut(s) 1664
MspI CCGG 8 cut(s) 197, 209, 227, 244, 741, 967, 1182, 1468
MspR9I CCNGG 9 cut(s) 695, 741, 761, 767, 967, 1134, 1182, 1352, 1468
MunI CAATTG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 1586
MvaI CCWGG 5 cut(s) 695, 761, 767, 1134, 1352
MvnI CGCG 7 cut(s) 318, 320, 330, 354, 364, 475, 1321
NaeI GCCGGC 2 cut(s) 210, 228
NarI GGCGCC 2 cut(s) 206, 224
NciI CCSGG 4 cut(s) 741, 967, 1182, 1468
NdeII GATC 5 cut(s) 402, 419, 816, 846, 1620
NgoMIV GCCGGC 2 cut(s) 208, 226
NlaIV GGNNCC 6 cut(s) 30, 38, 207, 225, 818, 862
NmuCI GTSAC 3 cut(s) 949, 959, 1334
NsiI ATGCAT 1 cut(s) 597
NspI RCATGY 5 cut(s) 629, 661, 807, 950, 1534
PacI TTAATTAA 1 cut(s) 273
PaeI GCATGC 1 cut(s) 807
PaeR7I CTCGAG 1 cut(s) 1006
PciI ACATGT 3 cut(s) 657, 946, 1530
PcsI WCGNNNNNNNCGW 1 cut(s) 1339
PctI GAATGC 1 cut(s) 1586
PdiI GCCGGC 2 cut(s) 210, 228
PdmI GAANNNNTTC 1 cut(s) 1082
PfeI GAWTC 1 cut(s) 485
PflFI GACNNNGTC 1 cut(s) 1370
PflMI CCANNNNNTGG 1 cut(s) 512
PkrI GCNGC 9 cut(s) 118, 202, 205, 220, 223, 317, 351, 940, 972
PleI GAGTC 1 cut(s) 956
PluTI GGCGCC 2 cut(s) 209, 227
PmaCI CACGTG 2 cut(s) 310, 783
PmlI CACGTG 2 cut(s) 310, 783
PpsI GAGTC 1 cut(s) 956
Ppu21I YACGTR 4 cut(s) 310, 783, 1056, 1230
PpuMI RGGWCCY 1 cut(s) 28
PscI ACATGT 3 cut(s) 657, 946, 1530
PsiI TTATAA 1 cut(s) 1493
Psp124BI GAGCTC 2 cut(s) 726, 931
Psp5II RGGWCCY 1 cut(s) 28
Psp6I CCWGG 5 cut(s) 693, 759, 765, 1132, 1350
PspCI CACGTG 2 cut(s) 310, 783
PspFI CCCAGC 1 cut(s) 896
PspGI CCWGG 5 cut(s) 693, 759, 765, 1132, 1350
PspN4I GGNNCC 6 cut(s) 30, 38, 207, 225, 818, 862
PspPI GGNCC 6 cut(s) 28, 313, 335, 372, 763, 1465
PspPPI RGGWCCY 1 cut(s) 28
PsuI RGATCY 1 cut(s) 816
PsyI GACNNNGTC 1 cut(s) 1370
RsaI GTAC 5 cut(s) 945, 1232, 1252, 1285, 1565
RsaNI GTAC 5 cut(s) 944, 1231, 1251, 1284, 1564
RseI CAYNNNNRTG 1 cut(s) 785
SacI GAGCTC 2 cut(s) 726, 931
SaqAI TTAA 8 cut(s) 269, 273, 279, 564, 1274, 1305, 1472, 1665
SatI GCNGC 9 cut(s) 117, 201, 204, 219, 222, 316, 350, 939, 971
Sau3AI GATC 5 cut(s) 402, 419, 816, 846, 1620
Sau96I GGNCC 6 cut(s) 28, 313, 335, 372, 763, 1465
ScaI AGTACT 1 cut(s) 1252
SchI GAGTC 1 cut(s) 956
ScrFI CCNGG 9 cut(s) 695, 741, 761, 767, 967, 1134, 1182, 1352, 1468
SduI GDGCHC 6 cut(s) 309, 726, 754, 773, 931, 1608
SexAI ACCWGGT 1 cut(s) 1350
SfaNI GCATC 4 cut(s) 626, 668, 702, 1743
SfoI GGCGCC 2 cut(s) 207, 225
Sfr274I CTCGAG 1 cut(s) 1006
SgrAI CRCCGGYG 2 cut(s) 208, 226
SinI GGWCC 2 cut(s) 28, 372
SlaI CTCGAG 1 cut(s) 1006
SmiMI CAYNNNNRTG 1 cut(s) 785
SmlI CTYRAG 4 cut(s) 917, 1006, 1449, 1664
SmoI CTYRAG 4 cut(s) 917, 1006, 1449, 1664
SnaBI TACGTA 1 cut(s) 1056
SpeI ACTAGT 1 cut(s) 1235
SphI GCATGC 1 cut(s) 807
SspDI GGCGCC 2 cut(s) 205, 223
SspI AATATT 2 cut(s) 262, 288
SspMI CTAG 7 cut(s) 81, 873, 932, 1140, 1148, 1236, 1500
SstI GAGCTC 2 cut(s) 726, 931
StyD4I CCNGG 9 cut(s) 693, 739, 759, 765, 965, 1132, 1180, 1350, 1466
StyI CCWWGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 546, 1557
TaiI ACGT 9 cut(s) 72, 142, 312, 427, 785, 956, 1058, 1232, 1336
TaqI TCGA 4 cut(s) 1007, 1480, 1527, 1608
TatI WGTACW 2 cut(s) 1250, 1563
TauI GCSGC 6 cut(s) 203, 206, 221, 224, 318, 352
TfiI GAWTC 1 cut(s) 485
Tru1I TTAA 8 cut(s) 269, 273, 279, 564, 1274, 1305, 1472, 1665
Tru9I TTAA 8 cut(s) 269, 273, 279, 564, 1274, 1305, 1472, 1665
TscAI CASTG 3 cut(s) 302, 309, 513
TseFI GTSAC 3 cut(s) 949, 959, 1334
TseI GCWGC 3 cut(s) 116, 938, 970
Tsp45I GTSAC 3 cut(s) 949, 959, 1334
TspDTI ATGAA 3 cut(s) 111, 617, 1056
TspGWI ACGGA 4 cut(s) 255, 281, 297, 517
TspRI CASTG 3 cut(s) 302, 309, 513
Tth111I GACNNNGTC 1 cut(s) 1370
Van91I CCANNNNNTGG 1 cut(s) 512
Vha464I CTTAAG 1 cut(s) 1664
VneI GTGCAC 1 cut(s) 305
VpaK11BI GGWCC 2 cut(s) 28, 372
XapI RAATTY 6 cut(s) 61, 144, 182, 379, 391, 560
XceI RCATGY 5 cut(s) 629, 661, 807, 950, 1534
XhoI CTCGAG 1 cut(s) 1006
XmiI GTMKAC 1 cut(s) 1374
XmnI GAANNNNTTC 1 cut(s) 1082
XspI CTAG 7 cut(s) 81, 873, 932, 1140, 1148, 1236, 1500
ZrmI AGTACT 1 cut(s) 1252
Zsp2I ATGCAT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.