pycom02g07390

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000513
Physical Location & Seq
Forward (+)
5191414 .. 5191701
288 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g07390.1

Sequence Viewer

Length: 288 bp
ATGTTTAGAGAGCTCATAATCTGCACCCCCGGTGTTAGTGCTCCCGCCCAGGGCCAGGGCACAGTCCTTCACGTGATGTTCACCAGCACCACACGCTCGCCTTGGATCCAAGTTAGGTGCAAGCCTTGTCGGACAGACTACATTAGGTGGTTCCGACTCGTAGGTGACCCGCGATTATTCGCACAGTCTTCACGTGATCGTAGCACTAGAGCGTATATATATTACACCCAGTCCTGTCGGACAGACTACATTAGGTGGTTCCGACTCGTGTGCAGATTCAGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

96

Amino Acids

11.35

Weight (kDa)

10.3

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 172
AciI CCGC 2 cut(s) 45, 170
AclWI GGATC 2 cut(s) 100, 113
AcvI CACGTG 2 cut(s) 73, 194
AfiI CCNNNNNNNGG 2 cut(s) 50, 55
AjnI CCWGG 2 cut(s) 48, 54
AluBI AGCT 1 cut(s) 13
AluI AGCT 1 cut(s) 13
Alw21I GWGCWC 2 cut(s) 15, 43
AlwI GGATC 2 cut(s) 100, 113
AoxI GGCC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 52
AsuC2I CCSGG 1 cut(s) 30
AsuHPI GGTGA 2 cut(s) 73, 176
BaeGI GKGCMC 1 cut(s) 62
BamHI GGATCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 15
BauI CACGAG 1 cut(s) 266
BbrPI CACGTG 2 cut(s) 73, 194
BbsI GAAGAC 1 cut(s) 180
Bbv12I GWGCWC 2 cut(s) 15, 43
BcgI CGANNNNNNTGC 2 cut(s) 252, 286
BciT130I CCWGG 2 cut(s) 50, 56
BcnI CCSGG 1 cut(s) 30
BfaI CTAG 1 cut(s) 207
Bme1390I CCNGG 3 cut(s) 30, 50, 56
BmgT120I GGNCC 1 cut(s) 52
BmiI GGNNCC 3 cut(s) 107, 152, 260
BmrFI CCNGG 3 cut(s) 30, 50, 56
BmrI ACTGGG 1 cut(s) 223
BmuI ACTGGG 1 cut(s) 223
BpiI GAAGAC 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 30
BsaAI YACGTR 2 cut(s) 73, 194
BsaJI CCNNGG 5 cut(s) 28, 48, 49, 55, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 55
Bse1I ACTGG 1 cut(s) 229
BseBI CCWGG 2 cut(s) 50, 56
BseDI CCNNGG 5 cut(s) 28, 48, 49, 55, 101
BseLI CCNNNNNNNGG 2 cut(s) 50, 55
BseNI ACTGG 1 cut(s) 229
BseSI GKGCMC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 7
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 1 cut(s) 54
BsiHKAI GWGCWC 2 cut(s) 15, 43
BsiSI CCGG 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 50, 55
BsnI GGCC 1 cut(s) 54
Bsp1286I GDGCHC 3 cut(s) 15, 43, 62
Bsp143I GATC 2 cut(s) 105, 196
BspACI CCGC 2 cut(s) 45, 170
BspANI GGCC 1 cut(s) 54
BspFNI CGCG 1 cut(s) 172
BspLI GGNNCC 3 cut(s) 107, 152, 260
BspPI GGATC 2 cut(s) 100, 113
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 5 cut(s) 28, 48, 49, 55, 101
BssMI GATC 2 cut(s) 105, 196
BssSI CACGAG 1 cut(s) 266
BssT1I CCWWGG 1 cut(s) 101
Bst2BI CACGAG 1 cut(s) 266
Bst2UI CCWGG 2 cut(s) 50, 56
Bst4CI ACNGT 2 cut(s) 64, 186
BstBAI YACGTR 2 cut(s) 73, 194
BstC8I GCNNGC 2 cut(s) 98, 122
BstEII GGTNACC 1 cut(s) 164
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 2 cut(s) 108, 199
BstMBI GATC 2 cut(s) 105, 196
BstMWI GCNNNNNNNGC 1 cut(s) 93
BstNI CCWGG 2 cut(s) 50, 56
BstPI GGTNACC 1 cut(s) 164
BstSCI CCNGG 3 cut(s) 28, 48, 54
BstSLI GKGCMC 1 cut(s) 62
BstUI CGCG 1 cut(s) 172
BstV2I GAAGAC 1 cut(s) 180
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 1 cut(s) 54
Cac8I GCNNGC 2 cut(s) 98, 122
Cfr13I GGNCC 1 cut(s) 52
CviJI RGCY 3 cut(s) 13, 54, 124
CviKI_1 RGCY 3 cut(s) 13, 54, 124
DpnI GATC 2 cut(s) 107, 198
DpnII GATC 2 cut(s) 105, 196
Ecl136II GAGCTC 1 cut(s) 13
Eco130I CCWWGG 1 cut(s) 101
Eco24I GRGCYC 1 cut(s) 15
Eco53kI GAGCTC 1 cut(s) 13
Eco72I CACGTG 2 cut(s) 73, 194
Eco91I GGTNACC 1 cut(s) 164
EcoICRI GAGCTC 1 cut(s) 13
EcoO65I GGTNACC 1 cut(s) 164
EcoRII CCWGG 2 cut(s) 48, 54
EcoT14I CCWWGG 1 cut(s) 101
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 101
FaiI YATR 4 cut(s) 17, 216, 218, 220
FauI CCCGC 2 cut(s) 52, 177
FriOI GRGCYC 1 cut(s) 15
FspBI CTAG 1 cut(s) 207
HaeIII GGCC 1 cut(s) 54
HapII CCGG 1 cut(s) 30
HinfI GANTC 3 cut(s) 156, 264, 276
HpaII CCGG 1 cut(s) 30
HphI GGTGA 2 cut(s) 73, 176
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 5 cut(s) 132, 155, 240, 263, 281
Hpy8I GTNNAC 1 cut(s) 81
HpyAV CCTTC 1 cut(s) 77
HpyCH4III ACNGT 2 cut(s) 64, 186
HpyCH4IV ACGT 2 cut(s) 72, 193
HpyCH4V TGCA 3 cut(s) 24, 120, 273
HpyF10VI GCNNNNNNNGC 1 cut(s) 93
HpySE526I ACGT 2 cut(s) 72, 193
Kzo9I GATC 2 cut(s) 105, 196
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 8 cut(s) 35, 41, 43, 62, 68, 97, 242, 247
MaeI CTAG 1 cut(s) 207
MaeII ACGT 2 cut(s) 72, 193
MaeIII GTNAC 1 cut(s) 164
MalI GATC 2 cut(s) 107, 198
MboI GATC 2 cut(s) 105, 196
MboII GAAGA 1 cut(s) 180
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 3 cut(s) 15, 43, 62
MlyI GAGTC 2 cut(s) 150, 258
MmeI TCCRAC 4 cut(s) 110, 178, 218, 286
MspI CCGG 1 cut(s) 30
MspR9I CCNGG 3 cut(s) 30, 50, 56
MvaI CCWGG 2 cut(s) 50, 56
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 93
NciI CCSGG 1 cut(s) 30
NdeII GATC 2 cut(s) 105, 196
NlaIV GGNNCC 3 cut(s) 107, 152, 260
NmuCI GTSAC 1 cut(s) 164
PasI CCCWGGG 1 cut(s) 49
PfeI GAWTC 1 cut(s) 276
PleI GAGTC 2 cut(s) 150, 258
PmaCI CACGTG 2 cut(s) 73, 194
PmlI CACGTG 2 cut(s) 73, 194
PpsI GAGTC 2 cut(s) 150, 258
Ppu21I YACGTR 2 cut(s) 73, 194
Psp124BI GAGCTC 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 48, 54
PspCI CACGTG 2 cut(s) 73, 194
PspEI GGTNACC 1 cut(s) 164
PspGI CCWGG 2 cut(s) 48, 54
PspN4I GGNNCC 3 cut(s) 107, 152, 260
PspPI GGNCC 1 cut(s) 52
PsuI RGATCY 1 cut(s) 105
SacI GAGCTC 1 cut(s) 15
Sau3AI GATC 2 cut(s) 105, 196
Sau96I GGNCC 1 cut(s) 52
SchI GAGTC 2 cut(s) 150, 258
ScrFI CCNGG 3 cut(s) 30, 50, 56
SduI GDGCHC 3 cut(s) 15, 43, 62
SetI ASST 7 cut(s) 15, 75, 119, 149, 166, 196, 257
SsiI CCGC 2 cut(s) 45, 170
SspMI CTAG 1 cut(s) 207
SstI GAGCTC 1 cut(s) 15
StyD4I CCNGG 3 cut(s) 28, 48, 54
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 2 cut(s) 64, 186
TaiI ACGT 2 cut(s) 75, 196
TfiI GAWTC 1 cut(s) 276
TseFI GTSAC 1 cut(s) 164
Tsp45I GTSAC 1 cut(s) 164
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.