pycom05g13560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000513
Physical Location & Seq
Forward (+)
17119440 .. 17120274
835 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g13560.1

Sequence Viewer

Length: 312 bp
ATGTACGGACCACACCCAAAATCCATCAAACCTTCACAGATCGTTAAATCCAAGCACATATTCGAGCTCGTGAGGCTTGTAGGAGTCAACCATACCTTTTTCAGGGAATTTCAAGCTTGTAGGAAGCTTGGTGAGGTCCCTAGGAGGCTCGGGGTGGTTCATTTGAAGGTTTTGGACGTCGAGATCACTAGTTTGGAAGTTGGCCGGAGATGGGTTGTTTTTGCAGTTGTTTCGCATATAGGTGATACCTATCCCGAGGACGAGCGTGGTCACTCGAGGCAGGGGGGCTACGACCCTTCCACATACCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

104

Amino Acids

11.81

Weight (kDa)

9.46

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 180
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 102, 211
AgsI TTSAA 2 cut(s) 113, 166
AhlI ACTAGT 1 cut(s) 188
AjuI GAANNNNNNNTTGG 2 cut(s) 44, 76
AluBI AGCT 3 cut(s) 67, 116, 127
AluI AGCT 3 cut(s) 67, 116, 127
Alw21I GWGCWC 1 cut(s) 69
Ama87I CYCGRG 3 cut(s) 149, 254, 274
AoxI GGCC 1 cut(s) 202
ApoI RAATTY 1 cut(s) 107
AspA2I CCTAGG 1 cut(s) 140
AspS9I GGNCC 2 cut(s) 8, 136
AsuHPI GGTGA 2 cut(s) 143, 254
AvaI CYCGRG 3 cut(s) 149, 254, 274
AvaII GGWCC 2 cut(s) 8, 136
AvrII CCTAGG 1 cut(s) 140
BanII GRGCYC 1 cut(s) 69
BauI CACGAG 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 69
BccI CCATC 2 cut(s) 32, 204
BcgI CGANNNNNNTGC 2 cut(s) 213, 247
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 2 cut(s) 141, 189
BlnI CCTAGG 1 cut(s) 140
Bme18I GGWCC 2 cut(s) 8, 136
BmeT110I CYCGRG 3 cut(s) 149, 254, 274
BmgT120I GGNCC 2 cut(s) 8, 136
BmiI GGNNCC 1 cut(s) 138
BsaBI GATNNNNATC 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 140, 255
Bsc4I CCNNNNNNNGG 2 cut(s) 102, 211
Bse1I ACTGG 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 140, 255
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 102, 211
BseNI ACTGG 1 cut(s) 307
BshFI GGCC 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 69
BsiHKCI CYCGRG 3 cut(s) 149, 254, 274
BsiSI CCGG 1 cut(s) 205
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 2 cut(s) 102, 211
BsmFI GGGAC 1 cut(s) 122
BsnI GGCC 1 cut(s) 204
BsoBI CYCGRG 3 cut(s) 149, 254, 274
Bsp1286I GDGCHC 1 cut(s) 69
Bsp143I GATC 2 cut(s) 39, 183
BspANI GGCC 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 138
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 2 cut(s) 140, 255
BssMI GATC 2 cut(s) 39, 183
BssNI GRCGYC 1 cut(s) 177
BssSI CACGAG 1 cut(s) 68
BssT1I CCWWGG 1 cut(s) 140
Bst2BI CACGAG 1 cut(s) 68
BstACI GRCGYC 1 cut(s) 177
BstENI CCTNNNNNAGG 1 cut(s) 100
BstKTI GATC 2 cut(s) 42, 186
BstMBI GATC 2 cut(s) 39, 183
BstMWI GCNNNNNNNGC 1 cut(s) 73
BsuRI GGCC 1 cut(s) 204
Cfr13I GGNCC 2 cut(s) 8, 136
Csp6I GTAC 1 cut(s) 4
CviJI RGCY 7 cut(s) 67, 76, 116, 127, 148, 204, 288
CviKI_1 RGCY 7 cut(s) 67, 76, 116, 127, 148, 204, 288
CviQI GTAC 1 cut(s) 4
DpnI GATC 2 cut(s) 41, 185
DpnII GATC 2 cut(s) 39, 183
EaeI YGGCCR 1 cut(s) 202
Ecl136II GAGCTC 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 140
Eco24I GRGCYC 1 cut(s) 69
Eco47I GGWCC 2 cut(s) 8, 136
Eco53kI GAGCTC 1 cut(s) 67
Eco88I CYCGRG 3 cut(s) 149, 254, 274
EcoICRI GAGCTC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 100
EcoO109I RGGNCCY 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 140
EcoT38I GRGCYC 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 140
FaiI YATR 5 cut(s) 59, 93, 237, 239, 304
FaqI GGGAC 1 cut(s) 122
FriOI GRGCYC 1 cut(s) 69
FspBI CTAG 2 cut(s) 141, 189
HaeIII GGCC 1 cut(s) 204
HapII CCGG 1 cut(s) 205
Hin1I GRCGYC 1 cut(s) 177
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 2 cut(s) 114, 125
HinfI GANTC 1 cut(s) 84
HpaII CCGG 1 cut(s) 205
HphI GGTGA 2 cut(s) 143, 254
Hpy166II GTNNAC 1 cut(s) 88
Hpy188III TCNNGA 3 cut(s) 70, 181, 254
Hpy8I GTNNAC 1 cut(s) 88
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 3 cut(s) 42, 160, 306
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 1 cut(s) 224
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 177
Kzo9I GATC 2 cut(s) 39, 183
LpnPI CCDG 3 cut(s) 88, 218, 266
MaeI CTAG 2 cut(s) 141, 189
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 1 cut(s) 269
MalI GATC 2 cut(s) 41, 185
MboI GATC 2 cut(s) 39, 183
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 1 cut(s) 107
MlyI GAGTC 1 cut(s) 93
MnlI CCTC 5 cut(s) 66, 127, 138, 250, 270
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 2 cut(s) 240, 307
MspI CCGG 1 cut(s) 205
MwoI GCNNNNNNNGC 1 cut(s) 73
NdeII GATC 2 cut(s) 39, 183
NlaIV GGNNCC 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 269
PaeR7I CTCGAG 1 cut(s) 274
PleI GAGTC 1 cut(s) 92
PpsI GAGTC 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 136
Psp124BI GAGCTC 1 cut(s) 69
Psp5II RGGWCCY 1 cut(s) 136
PspN4I GGNNCC 1 cut(s) 138
PspPI GGNCC 2 cut(s) 8, 136
PspPPI RGGWCCY 1 cut(s) 136
PspXI VCTCGAGB 1 cut(s) 274
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 2 cut(s) 240, 307
SacI GAGCTC 1 cut(s) 69
SaqAI TTAA 1 cut(s) 45
Sau3AI GATC 2 cut(s) 39, 183
Sau96I GGNCC 2 cut(s) 8, 136
SchI GAGTC 1 cut(s) 93
SduI GDGCHC 1 cut(s) 69
Sfr274I CTCGAG 1 cut(s) 274
SinI GGWCC 2 cut(s) 8, 136
SlaI CTCGAG 1 cut(s) 274
SmiMI CAYNNNNRTG 2 cut(s) 240, 307
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SpeI ACTAGT 1 cut(s) 188
Sse9I AATT 1 cut(s) 107
SspMI CTAG 2 cut(s) 141, 189
SstI GAGCTC 1 cut(s) 69
StyI CCWWGG 1 cut(s) 140
TaiI ACGT 1 cut(s) 180
TaqI TCGA 3 cut(s) 63, 180, 275
TasI AATT 1 cut(s) 107
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TseFI GTSAC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 149
TspGWI ACGGA 1 cut(s) 21
VpaK11BI GGWCC 2 cut(s) 8, 136
XagI CCTNNNNNAGG 1 cut(s) 100
XapI RAATTY 1 cut(s) 107
XhoI CTCGAG 1 cut(s) 274
XmaJI CCTAGG 1 cut(s) 140
XspI CTAG 2 cut(s) 141, 189
ZraI GACGTC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.