pycom520g00840

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000513
Physical Location & Seq
Forward (+)
666653 .. 668287
1635 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00840.7

Sequence Viewer

Length: 1239 bp
ATGAATTACAGTCTACCTTTCTTTAAAATTATGGCTAACTGGCTTCTTAGAAACTCTTTTTCTTCTCTCAGGGTCTCTAAAGTTTGTTTGTTGCTCTTTTTGCTTCTAAAACTTTATGCTGCACTTCTAACCAAGATAAAACTTTTGCTTCTTCAAAGTCCTAGTGGGGTGTCTGGGACAATAAATGTAACATCCCGTCCCGGAAATATCGAAACGTACATTGAAATTACGATTTTACCCCTAAATTGTTCGTTTACGATTAGTGTTGTGTTGTGTGGGTTGTGGGACCACACACACTCATACATTTTCTTTTTCTTCCCGGGATTCCCTCCCTCATTCCCTCACTCTGTCTTTCTCTGTCTCTCTCTCTCTCTCTCTCCCCCGAGTCTAACTCCTTCTTCTTCATTCAACACACAGACAACACACACATATACTCAAACCTTCACCAAACAAGAAACCAAGACCACCATCGTGTTCGTGGAGCTGAGAGGAGTTCAAAGGTGCCATTTTCAGGTAAGGATATCGTTGTTTTCACGTCGATATCGCCTTGGCCGAATTATGCACTCTTGTAGATGTGTTTGTGAGGTCCCAAGGAGCCTCGGAGTAGTTCGTTGGACGAATTTGGACGTTGGGAAGGCTAGGTTCAAAGTTGGCCGAACTTTGATTGTTGTTGCAGCTTCATTTTGGTATAAAGAACGTGAAATTTGGTTGAAAAACGAAGGAGAAAACAAAGTTGGAAAATTTCCCAGTTTTCCGGCGACGGCGGCGGCGCCGGCGACGGCGACGGCGGCGGCGCCGGCGACGGCGACGGCGACTGGCCGGAGAAGACAGGAGAATATTCCGTTAATTAAGGTTAACGGAATATTCCGTCAGTGTGCAGTGCACGTGGGCCGCGCGTGGGGGCGCGTAGGGCCGTGCCTGTGGCGGCGCGTGGGGGCGCGTGCGTGGTCCAAAAATTTTTCTAAAAATTTGTGTGTGATCCTGAGGTTGTGTAGATCACGTTGGTATATTCAATTGTCCAATTTGAGCAATGTTTTCGTAACTTTGTCGCGTATAGGTGATTCGTTTTCCGAAGACGAGCGTACACACTCGAGGCAGGGGGGCTACGACCCTTCTAATTATCAGTTATTTGTTTTATGCCATGCATTATATGAATGTTGTTTACGCATCATTGCATGTATTATTGGTGATATACATACATATATACATGTGTATTTGGTGCGGTGGATGCACAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

413

Amino Acids

46.91

Weight (kDa)

10.03

Isoelectric Point (pI)

55.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 501, 769, 793
AccI GTMKAC 1 cut(s) 13
AccII CGCG 6 cut(s) 894, 896, 906, 930, 940, 1051
AciI CCGC 7 cut(s) 764, 767, 788, 791, 892, 925, 1222
AclWI GGATC 1 cut(s) 973
AcoI YGGCCR 3 cut(s) 550, 652, 817
AcsI RAATTY 5 cut(s) 619, 702, 740, 955, 967
AcvI CACGTG 1 cut(s) 886
AcyI GRCGYC 2 cut(s) 770, 794
AfaI GTAC 2 cut(s) 218, 1084
AfiI CCNNNNNNNGG 2 cut(s) 511, 898
AflIII ACRYGT 1 cut(s) 1207
AgsI TTSAA 7 cut(s) 155, 224, 409, 497, 646, 712, 1013
AjiI CACGTC 1 cut(s) 536
AjuI GAANNNNNNNTTGG 2 cut(s) 717, 749
AleI CACNNNNGTG 1 cut(s) 470
AluBI AGCT 2 cut(s) 484, 677
AluI AGCT 2 cut(s) 484, 677
Alw21I GWGCWC 1 cut(s) 885
Alw26I GTCTC 2 cut(s) 79, 365
Alw44I GTGCAC 1 cut(s) 881
AlwI GGATC 1 cut(s) 973
Ama87I CYCGRG 3 cut(s) 319, 382, 1090
AoxI GGCC 5 cut(s) 550, 652, 817, 889, 911
ApaLI GTGCAC 1 cut(s) 881
ApeKI GCWGC 2 cut(s) 119, 674
ApoI RAATTY 5 cut(s) 619, 702, 740, 955, 967
AspLEI GCGC 6 cut(s) 772, 796, 896, 906, 930, 940
AspS9I GGNCC 5 cut(s) 286, 586, 889, 911, 948
AsuC2I CCSGG 3 cut(s) 201, 320, 321
AsuHPI GGTGA 3 cut(s) 436, 1070, 1199
AvaI CYCGRG 3 cut(s) 319, 382, 1090
AvaII GGWCC 3 cut(s) 286, 586, 948
AxyI CCTNAGG 1 cut(s) 983
BaeGI GKGCMC 1 cut(s) 885
BanI GGYRCC 3 cut(s) 501, 769, 793
BbrPI CACGTG 1 cut(s) 886
BbsI GAAGAC 2 cut(s) 832, 1080
Bbv12I GWGCWC 1 cut(s) 885
BbvI GCAGC 2 cut(s) 106, 686
BccI CCATC 1 cut(s) 476
BceAI ACGGC 6 cut(s) 777, 795, 801, 819, 825, 898
BcgI CGANNNNNNTGC 2 cut(s) 1018, 1052
BcnI CCSGG 3 cut(s) 201, 320, 321
BcoDI GTCTC 2 cut(s) 79, 365
BfaI CTAG 2 cut(s) 162, 639
BfoI RGCGCY 2 cut(s) 773, 797
BisI GCNGC 8 cut(s) 120, 675, 765, 768, 789, 792, 892, 926
BlsI GCNGC 8 cut(s) 121, 676, 766, 769, 790, 793, 893, 927
Bme1390I CCNGG 3 cut(s) 201, 320, 321
Bme18I GGWCC 3 cut(s) 286, 586, 948
BmeT110I CYCGRG 3 cut(s) 319, 382, 1090
BmgBI CACGTC 1 cut(s) 536
BmgT120I GGNCC 5 cut(s) 286, 586, 889, 911, 948
BmiI GGNNCC 6 cut(s) 287, 503, 588, 596, 771, 795
BmrFI CCNGG 3 cut(s) 201, 320, 321
BmrI ACTGGG 1 cut(s) 741
BmsI GCATC 2 cut(s) 1176, 1218
BmuI ACTGGG 1 cut(s) 741
BpiI GAAGAC 2 cut(s) 832, 1080
BplI GAGNNNNNCTC 2 cut(s) 376, 408
BpuMI CCSGG 3 cut(s) 201, 320, 321
BsaAI YACGTR 1 cut(s) 886
BsaHI GRCGYC 2 cut(s) 770, 794
BsaI GGTCTC 1 cut(s) 79
BsaJI CCNNGG 4 cut(s) 319, 547, 590, 598
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 898
Bse118I RCCGGY 2 cut(s) 772, 796
Bse1I ACTGG 3 cut(s) 44, 747, 820
Bse21I CCTNAGG 1 cut(s) 983
Bse3DI GCAATG 2 cut(s) 1036, 1170
BseDI CCNNGG 4 cut(s) 319, 547, 590, 598
BseGI GGATG 2 cut(s) 191, 1233
BseLI CCNNNNNNNGG 2 cut(s) 511, 898
BseMI GCAATG 2 cut(s) 1036, 1170
BseMII CTCAG 3 cut(s) 82, 476, 974
BseNI ACTGG 3 cut(s) 44, 747, 820
BseRI GAGGAG 1 cut(s) 504
BseSI GKGCMC 1 cut(s) 885
BseXI GCAGC 2 cut(s) 106, 686
BsgI GTGCAG 2 cut(s) 105, 897
Bsh1236I CGCG 6 cut(s) 894, 896, 906, 930, 940, 1051
BshFI GGCC 5 cut(s) 552, 654, 819, 891, 913
BshNI GGYRCC 3 cut(s) 501, 769, 793
BsiHKAI GWGCWC 1 cut(s) 885
BsiHKCI CYCGRG 3 cut(s) 319, 382, 1090
BsiSI CCGG 6 cut(s) 201, 320, 755, 773, 797, 820
BslFI GGGAC 4 cut(s) 183, 190, 299, 572
BslI CCNNNNNNNGG 2 cut(s) 511, 898
BsmAI GTCTC 2 cut(s) 79, 365
BsmFI GGGAC 4 cut(s) 183, 190, 299, 572
BsnI GGCC 5 cut(s) 552, 654, 819, 891, 913
Bso31I GGTCTC 1 cut(s) 79
BsoBI CYCGRG 3 cut(s) 319, 382, 1090
Bsp1286I GDGCHC 1 cut(s) 885
Bsp143I GATC 2 cut(s) 978, 995
BspACI CCGC 7 cut(s) 764, 767, 788, 791, 892, 925, 1222
BspANI GGCC 5 cut(s) 552, 654, 819, 891, 913
BspCNI CTCAG 3 cut(s) 81, 477, 975
BspFNI CGCG 6 cut(s) 894, 896, 906, 930, 940, 1051
BspLI GGNNCC 6 cut(s) 287, 503, 588, 596, 771, 795
BspPI GGATC 1 cut(s) 973
BspT107I GGYRCC 3 cut(s) 501, 769, 793
BspTNI GGTCTC 1 cut(s) 79
BsrDI GCAATG 2 cut(s) 1036, 1170
BsrFI RCCGGY 2 cut(s) 772, 796
BsrI ACTGG 3 cut(s) 44, 747, 820
BssAI RCCGGY 2 cut(s) 772, 796
BssECI CCNNGG 4 cut(s) 319, 547, 590, 598
BssMI GATC 2 cut(s) 978, 995
BssNI GRCGYC 2 cut(s) 770, 794
BssT1I CCWWGG 2 cut(s) 547, 590
Bst4CI ACNGT 1 cut(s) 11
BstACI GRCGYC 2 cut(s) 770, 794
BstBAI YACGTR 1 cut(s) 886
BstC8I GCNNGC 3 cut(s) 774, 798, 942
BstDEI CTNAG 4 cut(s) 47, 68, 485, 983
BstF5I GGATG 2 cut(s) 191, 1233
BstFNI CGCG 6 cut(s) 894, 896, 906, 930, 940, 1051
BstH2I RGCGCY 2 cut(s) 773, 797
BstHHI GCGC 6 cut(s) 772, 796, 896, 906, 930, 940
BstKTI GATC 2 cut(s) 981, 998
BstMAI GTCTC 2 cut(s) 79, 365
BstMBI GATC 2 cut(s) 978, 995
BstMWI GCNNNNNNNGC 7 cut(s) 100, 764, 773, 788, 797, 910, 1228
BstNSI RCATGY 2 cut(s) 1179, 1211
BstSCI CCNGG 3 cut(s) 199, 318, 319
BstSLI GKGCMC 1 cut(s) 885
BstUI CGCG 6 cut(s) 894, 896, 906, 930, 940, 1051
BstV1I GCAGC 2 cut(s) 106, 686
BstV2I GAAGAC 2 cut(s) 832, 1080
Bsu36I CCTNAGG 1 cut(s) 983
BsuRI GGCC 5 cut(s) 552, 654, 819, 891, 913
BtrI CACGTC 1 cut(s) 536
BtsCI GGATG 2 cut(s) 191, 1233
BtsI GCAGTG 1 cut(s) 885
BtsIMutI CAGTG 2 cut(s) 878, 885
Cac8I GCNNGC 3 cut(s) 774, 798, 942
CfoI GCGC 6 cut(s) 772, 796, 896, 906, 930, 940
Cfr10I RCCGGY 2 cut(s) 772, 796
Cfr13I GGNCC 5 cut(s) 286, 586, 889, 911, 948
Cfr9I CCCGGG 1 cut(s) 319
Csp6I GTAC 2 cut(s) 217, 1083
CviAII CATG 3 cut(s) 1142, 1176, 1208
CviQI GTAC 2 cut(s) 217, 1083
DdeI CTNAG 4 cut(s) 47, 68, 485, 983
DinI GGCGCC 2 cut(s) 771, 795
DpnI GATC 2 cut(s) 980, 997
DpnII GATC 2 cut(s) 978, 995
DraI TTTAAA 1 cut(s) 25
EaeI YGGCCR 3 cut(s) 550, 652, 817
Eco130I CCWWGG 2 cut(s) 547, 590
Eco31I GGTCTC 1 cut(s) 79
Eco32I GATATC 2 cut(s) 522, 542
Eco47I GGWCC 3 cut(s) 286, 586, 948
Eco72I CACGTG 1 cut(s) 886
Eco81I CCTNAGG 1 cut(s) 983
Eco88I CYCGRG 3 cut(s) 319, 382, 1090
EcoO109I RGGNCCY 1 cut(s) 586
EcoRV GATATC 2 cut(s) 522, 542
EcoT14I CCWWGG 2 cut(s) 547, 590
EcoT22I ATGCAT 1 cut(s) 1147
EgeI GGCGCC 2 cut(s) 771, 795
EheI GGCGCC 2 cut(s) 771, 795
ErhI CCWWGG 2 cut(s) 547, 590
FaeI CATG 3 cut(s) 1145, 1179, 1211
FaqI GGGAC 4 cut(s) 183, 190, 299, 572
FatI CATG 3 cut(s) 1141, 1175, 1207
FblI GTMKAC 1 cut(s) 13
Fnu4HI GCNGC 8 cut(s) 120, 675, 765, 768, 789, 792, 892, 926
FokI GGATG 1 cut(s) 178
Fsp4HI GCNGC 8 cut(s) 120, 675, 765, 768, 789, 792, 892, 926
FspBI CTAG 2 cut(s) 162, 639
GlaI GCGC 6 cut(s) 771, 795, 895, 905, 929, 939
GluI GCNGC 8 cut(s) 120, 675, 765, 768, 789, 792, 892, 926
HaeII RGCGCY 2 cut(s) 773, 797
HaeIII GGCC 5 cut(s) 552, 654, 819, 891, 913
HapII CCGG 6 cut(s) 201, 320, 755, 773, 797, 820
HhaI GCGC 6 cut(s) 772, 796, 896, 906, 930, 940
Hin1I GRCGYC 2 cut(s) 770, 794
Hin1II CATG 3 cut(s) 1145, 1179, 1211
Hin6I GCGC 6 cut(s) 770, 794, 894, 904, 928, 938
HinP1I GCGC 6 cut(s) 770, 794, 894, 904, 928, 938
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HinfI GANTC 3 cut(s) 324, 385, 1061
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 6 cut(s) 201, 320, 755, 773, 797, 820
HphI GGTGA 3 cut(s) 436, 1070, 1199
Hpy166II GTNNAC 6 cut(s) 14, 255, 856, 883, 1085, 1163
Hpy188I TCNGA 2 cut(s) 602, 1072
Hpy188III TCNNGA 1 cut(s) 982
Hpy8I GTNNAC 6 cut(s) 14, 255, 856, 883, 1085, 1163
Hpy99I CGWCG 6 cut(s) 540, 763, 781, 787, 805, 811
HpyAV CCTTC 5 cut(s) 405, 451, 628, 713, 1122
HpyCH4III ACNGT 1 cut(s) 11
HpyCH4IV ACGT 6 cut(s) 215, 535, 627, 697, 885, 1000
HpyCH4V TGCA 8 cut(s) 122, 562, 674, 878, 883, 1145, 1175, 1231
HpyF10VI GCNNNNNNNGC 7 cut(s) 100, 764, 773, 788, 797, 910, 1228
HpyF3I CTNAG 4 cut(s) 47, 68, 485, 983
HpySE526I ACGT 6 cut(s) 215, 535, 627, 697, 885, 1000
Hsp92I GRCGYC 2 cut(s) 770, 794
Hsp92II CATG 3 cut(s) 1145, 1179, 1211
HspAI GCGC 6 cut(s) 770, 794, 894, 904, 928, 938
KasI GGCGCC 2 cut(s) 769, 793
KroI GCCGGC 2 cut(s) 772, 796
KroNI GCCGGC 2 cut(s) 774, 798
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 2 cut(s) 978, 995
LmnI GCTCC 2 cut(s) 481, 594
Lsp1109I GCAGC 2 cut(s) 106, 686
LweI GCATC 2 cut(s) 1176, 1218
MaeI CTAG 2 cut(s) 162, 639
MaeII ACGT 6 cut(s) 215, 535, 627, 697, 885, 1000
MaeIII GTNAC 2 cut(s) 187, 1039
MalI GATC 2 cut(s) 980, 997
MboI GATC 2 cut(s) 978, 995
MboII GAAGA 7 cut(s) 54, 143, 307, 390, 393, 837, 1085
MfeI CAATTG 1 cut(s) 1013
MhlI GDGCHC 1 cut(s) 885
Mly113I GGCGCC 2 cut(s) 770, 794
MlyI GAGTC 1 cut(s) 394
MmeI TCCRAC 2 cut(s) 593, 715
MnlI CCTC 8 cut(s) 339, 343, 351, 482, 577, 608, 978, 1086
Mph1103I ATGCAT 1 cut(s) 1147
MreI CGCCGGCG 2 cut(s) 772, 796
MroNI GCCGGC 2 cut(s) 772, 796
MseI TTAA 4 cut(s) 24, 845, 849, 855
MslI CAYNNNNRTG 1 cut(s) 470
MspI CCGG 6 cut(s) 201, 320, 755, 773, 797, 820
MspR9I CCNGG 3 cut(s) 201, 320, 321
MunI CAATTG 1 cut(s) 1013
MvnI CGCG 6 cut(s) 894, 896, 906, 930, 940, 1051
MwoI GCNNNNNNNGC 7 cut(s) 100, 764, 773, 788, 797, 910, 1228
NaeI GCCGGC 2 cut(s) 774, 798
NarI GGCGCC 2 cut(s) 770, 794
NciI CCSGG 3 cut(s) 201, 320, 321
NdeII GATC 2 cut(s) 978, 995
NgoMIV GCCGGC 2 cut(s) 772, 796
NlaIII CATG 3 cut(s) 1145, 1179, 1211
NlaIV GGNNCC 6 cut(s) 287, 503, 588, 596, 771, 795
NsiI ATGCAT 1 cut(s) 1147
NspI RCATGY 2 cut(s) 1179, 1211
OliI CACNNNNGTG 1 cut(s) 470
PacI TTAATTAA 1 cut(s) 849
PaeR7I CTCGAG 1 cut(s) 1090
PciI ACATGT 1 cut(s) 1207
PcsI WCGNNNNNNNCGW 2 cut(s) 550, 809
PdiI GCCGGC 2 cut(s) 774, 798
PfeI GAWTC 2 cut(s) 324, 1061
PfoI TCCNGGA 1 cut(s) 199
PkrI GCNGC 8 cut(s) 121, 676, 766, 769, 790, 793, 893, 927
PleI GAGTC 1 cut(s) 393
PluTI GGCGCC 2 cut(s) 773, 797
PmaCI CACGTG 1 cut(s) 886
PmlI CACGTG 1 cut(s) 886
PpsI GAGTC 1 cut(s) 393
Ppu21I YACGTR 1 cut(s) 886
PpuMI RGGWCCY 1 cut(s) 586
PscI ACATGT 1 cut(s) 1207
Psp5II RGGWCCY 1 cut(s) 586
PspCI CACGTG 1 cut(s) 886
PspN4I GGNNCC 6 cut(s) 287, 503, 588, 596, 771, 795
PspPI GGNCC 5 cut(s) 286, 586, 889, 911, 948
PspPPI RGGWCCY 1 cut(s) 586
PspXI VCTCGAGB 1 cut(s) 1090
RsaI GTAC 2 cut(s) 218, 1084
RsaNI GTAC 2 cut(s) 217, 1083
RseI CAYNNNNRTG 1 cut(s) 470
SaqAI TTAA 4 cut(s) 24, 845, 849, 855
SatI GCNGC 8 cut(s) 120, 675, 765, 768, 789, 792, 892, 926
Sau3AI GATC 2 cut(s) 978, 995
Sau96I GGNCC 5 cut(s) 286, 586, 889, 911, 948
SchI GAGTC 1 cut(s) 394
ScrFI CCNGG 3 cut(s) 201, 320, 321
SduI GDGCHC 1 cut(s) 885
SfaNI GCATC 2 cut(s) 1176, 1218
SfoI GGCGCC 2 cut(s) 771, 795
Sfr274I CTCGAG 1 cut(s) 1090
SgrAI CRCCGGYG 2 cut(s) 772, 796
SinI GGWCC 3 cut(s) 286, 586, 948
SlaI CTCGAG 1 cut(s) 1090
SmaI CCCGGG 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 470
SmlI CTYRAG 1 cut(s) 1090
SmoI CTYRAG 1 cut(s) 1090
SsiI CCGC 7 cut(s) 764, 767, 788, 791, 892, 925, 1222
SspDI GGCGCC 2 cut(s) 769, 793
SspI AATATT 2 cut(s) 838, 864
SspMI CTAG 2 cut(s) 162, 639
StyD4I CCNGG 3 cut(s) 199, 318, 319
StyI CCWWGG 2 cut(s) 547, 590
TaaI ACNGT 1 cut(s) 11
TaiI ACGT 6 cut(s) 218, 538, 630, 700, 888, 1003
TaqI TCGA 3 cut(s) 210, 538, 1091
TauI GCSGC 6 cut(s) 767, 770, 791, 794, 894, 928
TfiI GAWTC 2 cut(s) 324, 1061
Tru1I TTAA 4 cut(s) 24, 845, 849, 855
Tru9I TTAA 4 cut(s) 24, 845, 849, 855
TscAI CASTG 2 cut(s) 878, 885
TseI GCWGC 2 cut(s) 119, 674
TspDTI ATGAA 4 cut(s) 17, 393, 669, 1167
TspGWI ACGGA 3 cut(s) 831, 857, 873
TspMI CCCGGG 1 cut(s) 319
TspRI CASTG 2 cut(s) 878, 885
VneI GTGCAC 1 cut(s) 881
VpaK11BI GGWCC 3 cut(s) 286, 586, 948
XapI RAATTY 5 cut(s) 619, 702, 740, 955, 967
XceI RCATGY 2 cut(s) 1179, 1211
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XhoI CTCGAG 1 cut(s) 1090
XmaI CCCGGG 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 13
XspI CTAG 2 cut(s) 162, 639
Zsp2I ATGCAT 1 cut(s) 1147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.