MD13G1075700.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000513
Physical Location & Seq
Forward (+)
5352387 .. 5352682
296 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1075700.v1.1.491

Sequence Viewer

Length: 213 bp
ATGGACACATTCACCCATGATCATCCAAGCACGGATCGAAGGTTTCAAGGGCACCATTGTACTCGTGAGGACCCTACGAACTCACCCATACCCATTTTAGGACAATCCAAGCTCATGGTCAGCTTTAGGAGGTTCTTACGAAGCTCGGGGACGTTCGTTTGGAAGTTTTGGACGTCGGGATCACAAGGTTCGAAGGTGGCCAGTTTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

71

Amino Acids

7.99

Weight (kDa)

10.26

Isoelectric Point (pI)

57.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 51
AclWI GGATC 2 cut(s) 42, 187
AcoI YGGCCR 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 173
AfaI GTAC 1 cut(s) 61
AfiI CCNNNNNNNGG 2 cut(s) 98, 207
AgsI TTSAA 1 cut(s) 47
AluBI AGCT 3 cut(s) 112, 123, 144
AluI AGCT 3 cut(s) 112, 123, 144
AlwI GGATC 2 cut(s) 42, 187
Ama87I CYCGRG 1 cut(s) 145
AoxI GGCC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 70
AsuHPI GGTGA 2 cut(s) 4, 75
AsuII TTCGAA 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 145
AvaII GGWCC 1 cut(s) 70
BaeGI GKGCMC 1 cut(s) 54
BalI TGGCCA 1 cut(s) 200
BanI GGYRCC 1 cut(s) 51
BauI CACGAG 1 cut(s) 63
BclI TGATCA 1 cut(s) 19
Bme18I GGWCC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 145
BmgT120I GGNCC 1 cut(s) 70
BmiI GGNNCC 2 cut(s) 53, 72
Bpu14I TTCGAA 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 173
Bsc4I CCNNNNNNNGG 2 cut(s) 98, 207
Bse1I ACTGG 1 cut(s) 201
BseGI GGATG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 98, 207
BseNI ACTGG 1 cut(s) 201
BseSI GKGCMC 1 cut(s) 54
BshFI GGCC 1 cut(s) 200
BshNI GGYRCC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 145
BslFI GGGAC 1 cut(s) 163
BslI CCNNNNNNNGG 2 cut(s) 98, 207
BsmFI GGGAC 1 cut(s) 163
BsnI GGCC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 145
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 3 cut(s) 19, 34, 179
BspANI GGCC 1 cut(s) 200
BspLI GGNNCC 2 cut(s) 53, 72
BspPI GGATC 2 cut(s) 42, 187
BspT104I TTCGAA 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 51
BsrI ACTGG 1 cut(s) 201
BssMI GATC 3 cut(s) 19, 34, 179
BssNI GRCGYC 1 cut(s) 173
BssSI CACGAG 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 63
BstACI GRCGYC 1 cut(s) 173
BstBI TTCGAA 1 cut(s) 191
BstF5I GGATG 1 cut(s) 22
BstKTI GATC 3 cut(s) 22, 37, 182
BstMBI GATC 3 cut(s) 19, 34, 179
BstSLI GKGCMC 1 cut(s) 54
BstXI CCANNNNNNTGG 1 cut(s) 115
BsuRI GGCC 1 cut(s) 200
BtsCI GGATG 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 70
Csp6I GTAC 1 cut(s) 60
CviAII CATG 2 cut(s) 17, 115
CviJI RGCY 4 cut(s) 112, 123, 144, 200
CviKI_1 RGCY 4 cut(s) 112, 123, 144, 200
CviQI GTAC 1 cut(s) 60
DpnI GATC 3 cut(s) 21, 36, 181
DpnII GATC 3 cut(s) 19, 34, 179
EaeI YGGCCR 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 70
Eco88I CYCGRG 1 cut(s) 145
EcoO109I RGGNCCY 1 cut(s) 70
FaeI CATG 2 cut(s) 20, 118
FaiI YATR 3 cut(s) 18, 89, 116
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 16, 114
FbaI TGATCA 1 cut(s) 19
FokI GGATG 1 cut(s) 9
HaeIII GGCC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 173
Hin1II CATG 2 cut(s) 20, 118
HphI GGTGA 2 cut(s) 4, 75
Hpy188III TCNNGA 2 cut(s) 65, 177
Hpy99I CGWCG 1 cut(s) 178
HpyAV CCTTC 2 cut(s) 33, 187
HpyCH4IV ACGT 2 cut(s) 152, 173
HpySE526I ACGT 2 cut(s) 152, 173
Hsp92I GRCGYC 1 cut(s) 173
Hsp92II CATG 2 cut(s) 20, 118
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 3 cut(s) 19, 34, 179
MaeII ACGT 2 cut(s) 152, 173
MalI GATC 3 cut(s) 21, 36, 181
MboI GATC 3 cut(s) 19, 34, 179
MhlI GDGCHC 1 cut(s) 54
MlsI TGGCCA 1 cut(s) 200
MluNI TGGCCA 1 cut(s) 200
MnlI CCTC 2 cut(s) 61, 123
Mox20I TGGCCA 1 cut(s) 200
MscI TGGCCA 1 cut(s) 200
Msp20I TGGCCA 1 cut(s) 200
NdeII GATC 3 cut(s) 19, 34, 179
NlaIII CATG 2 cut(s) 20, 118
NlaIV GGNNCC 2 cut(s) 53, 72
NspV TTCGAA 1 cut(s) 191
PpuMI RGGWCCY 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 53, 72
PspPI GGNCC 1 cut(s) 70
PspPPI RGGWCCY 1 cut(s) 70
RsaI GTAC 1 cut(s) 61
RsaNI GTAC 1 cut(s) 60
Sau3AI GATC 3 cut(s) 19, 34, 179
Sau96I GGNCC 1 cut(s) 70
SduI GDGCHC 1 cut(s) 54
SetI ASST 9 cut(s) 44, 114, 125, 134, 146, 155, 176, 190, 198
SfuI TTCGAA 1 cut(s) 191
SinI GGWCC 1 cut(s) 70
TaiI ACGT 2 cut(s) 155, 176
TaqI TCGA 2 cut(s) 37, 191
TatI WGTACW 1 cut(s) 59
TspGWI ACGGA 1 cut(s) 47
VpaK11BI GGWCC 1 cut(s) 70
ZraI GACGTC 1 cut(s) 174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.