pycom13g24500

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000513
Physical Location & Seq
Forward (+)
21616739 .. 21617594
856 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g24500.1

Sequence Viewer

Length: 663 bp
ATGGTTGAAAAACGAAGGAGAAAACTTAGTTTGAAAATTACCCAGTTTTCCGGCGCCGTCGCCGGCGCCGGCGTCTCGCCGGAGAAGAAAGGGGAATATTCCGTTAAGTTTAACGGAATATTCCTAATGGCAGTGGACGGCATCTGGTTAGATTTAACGGAATATTCCTGTGCAGTGCACGTGGCCGCGCATGGGGGGCGCGTAGGTCCGTGCCTGTTCAGGCGCGTGGGGGCGCGTGCGTGGTCCAAAAATTTTTCTAAAAATATGGGTGTGATCCTGAGGTTGTGTAGGTCACGTTGGTATATTCAATTTAACGTTTTCGTAACTTTGTCGCGTTTAGGTGATTCGTATTCCGAGGACGAGCGTACACACTCGAGGCAGGGGGGCTACGACCCTTCTAATTACCATAATTCCCAGAAATTAAATAGAAAAGGTTATTTGTTTTATGCCATGCATCATATAAATGTTGTTTACGCATCATTGCATGTATTAGTAGTGGCATACATATATATATATATGTGTATTTGGTGCTGTGGACGCACAGGTAAGTTTAGAGAGCTCATAATCTGCACCCCCGGTGTTAGTGCTCCCGTCCAGGGCCAGGGCACAGCCTTCACGTGTATGTTCACCAGCACCACATGCTCGTCTTGGATCCATAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

221

Amino Acids

24.82

Weight (kDa)

9.61

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 53, 65
AccII CGCG 5 cut(s) 188, 201, 225, 235, 334
AciI CCGC 1 cut(s) 186
AclI AACGTT 1 cut(s) 315
AclWI GGATC 3 cut(s) 268, 646, 659
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 1 cut(s) 250
AcvI CACGTG 2 cut(s) 181, 618
AcyI GRCGYC 3 cut(s) 54, 66, 72
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 3 cut(s) 192, 596, 601
AflIII ACRYGT 1 cut(s) 617
AgsI TTSAA 3 cut(s) 8, 34, 308
AjnI CCWGG 2 cut(s) 594, 600
AluBI AGCT 1 cut(s) 559
AluI AGCT 1 cut(s) 559
Alw21I GWGCWC 3 cut(s) 180, 561, 589
Alw26I GTCTC 1 cut(s) 79
Alw44I GTGCAC 1 cut(s) 176
AlwI GGATC 3 cut(s) 268, 646, 659
Ama87I CYCGRG 1 cut(s) 373
AoxI GGCC 2 cut(s) 183, 598
ApaLI GTGCAC 1 cut(s) 176
ApoI RAATTY 1 cut(s) 250
AspLEI GCGC 6 cut(s) 56, 68, 190, 201, 225, 235
AspS9I GGNCC 3 cut(s) 206, 243, 598
AsuC2I CCSGG 1 cut(s) 576
AsuHPI GGTGA 2 cut(s) 353, 619
AvaI CYCGRG 1 cut(s) 373
AvaII GGWCC 2 cut(s) 206, 243
AxyI CCTNAGG 1 cut(s) 278
BaeGI GKGCMC 2 cut(s) 180, 608
BamHI GGATCC 1 cut(s) 651
BanI GGYRCC 2 cut(s) 53, 65
BanII GRGCYC 1 cut(s) 561
BbrPI CACGTG 2 cut(s) 181, 618
Bbv12I GWGCWC 3 cut(s) 180, 561, 589
BceAI ACGGC 2 cut(s) 41, 154
BciT130I CCWGG 2 cut(s) 596, 602
BcnI CCSGG 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 79
BfoI RGCGCY 2 cut(s) 57, 69
BisI GCNGC 1 cut(s) 186
BlsI GCNGC 1 cut(s) 187
Bme1390I CCNGG 3 cut(s) 576, 596, 602
Bme18I GGWCC 2 cut(s) 206, 243
BmeT110I CYCGRG 1 cut(s) 373
BmgT120I GGNCC 3 cut(s) 206, 243, 598
BmiI GGNNCC 3 cut(s) 55, 67, 653
BmrFI CCNGG 3 cut(s) 576, 596, 602
BmrI ACTGGG 1 cut(s) 37
BmsI GCATC 3 cut(s) 150, 463, 485
BmuI ACTGGG 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 576
BsaAI YACGTR 2 cut(s) 181, 618
BsaHI GRCGYC 3 cut(s) 54, 66, 72
BsaJI CCNNGG 4 cut(s) 354, 574, 595, 601
Bsc4I CCNNNNNNNGG 3 cut(s) 192, 596, 601
Bse118I RCCGGY 2 cut(s) 62, 68
Bse1I ACTGG 1 cut(s) 43
Bse21I CCTNAGG 1 cut(s) 278
Bse3DI GCAATG 1 cut(s) 479
BseBI CCWGG 2 cut(s) 596, 602
BseDI CCNNGG 4 cut(s) 354, 574, 595, 601
BseLI CCNNNNNNNGG 3 cut(s) 192, 596, 601
BseMI GCAATG 1 cut(s) 479
BseMII CTCAG 1 cut(s) 269
BseNI ACTGG 1 cut(s) 43
BseSI GKGCMC 2 cut(s) 180, 608
BsgI GTGCAG 2 cut(s) 192, 553
Bsh1236I CGCG 5 cut(s) 188, 201, 225, 235, 334
BshFI GGCC 2 cut(s) 185, 600
BshNI GGYRCC 2 cut(s) 53, 65
BsiHKAI GWGCWC 3 cut(s) 180, 561, 589
BsiHKCI CYCGRG 1 cut(s) 373
BsiSI CCGG 5 cut(s) 51, 63, 69, 80, 576
BslI CCNNNNNNNGG 3 cut(s) 192, 596, 601
BsmAI GTCTC 1 cut(s) 79
BsmBI CGTCTC 1 cut(s) 79
BsnI GGCC 2 cut(s) 185, 600
BsoBI CYCGRG 1 cut(s) 373
Bsp1286I GDGCHC 4 cut(s) 180, 561, 589, 608
Bsp143I GATC 2 cut(s) 273, 651
BspACI CCGC 1 cut(s) 186
BspANI GGCC 2 cut(s) 185, 600
BspCNI CTCAG 1 cut(s) 270
BspFNI CGCG 5 cut(s) 188, 201, 225, 235, 334
BspLI GGNNCC 3 cut(s) 55, 67, 653
BspPI GGATC 3 cut(s) 268, 646, 659
BspT107I GGYRCC 2 cut(s) 53, 65
BsrDI GCAATG 1 cut(s) 479
BsrFI RCCGGY 2 cut(s) 62, 68
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 2 cut(s) 62, 68
BssECI CCNNGG 4 cut(s) 354, 574, 595, 601
BssMI GATC 2 cut(s) 273, 651
BssNI GRCGYC 3 cut(s) 54, 66, 72
Bst2UI CCWGG 2 cut(s) 596, 602
BstACI GRCGYC 3 cut(s) 54, 66, 72
BstAPI GCANNNNNTGC 1 cut(s) 639
BstBAI YACGTR 2 cut(s) 181, 618
BstC8I GCNNGC 3 cut(s) 64, 70, 237
BstDEI CTNAG 2 cut(s) 26, 278
BstFNI CGCG 5 cut(s) 188, 201, 225, 235, 334
BstH2I RGCGCY 2 cut(s) 57, 69
BstHHI GCGC 6 cut(s) 56, 68, 190, 201, 225, 235
BstKTI GATC 2 cut(s) 276, 654
BstMAI GTCTC 1 cut(s) 79
BstMBI GATC 2 cut(s) 273, 651
BstMWI GCNNNNNNNGC 3 cut(s) 196, 537, 639
BstNI CCWGG 2 cut(s) 596, 602
BstNSI RCATGY 2 cut(s) 488, 642
BstSCI CCNGG 3 cut(s) 574, 594, 600
BstSLI GKGCMC 2 cut(s) 180, 608
BstUI CGCG 5 cut(s) 188, 201, 225, 235, 334
BstX2I RGATCY 1 cut(s) 651
BstYI RGATCY 1 cut(s) 651
Bsu36I CCTNAGG 1 cut(s) 278
BsuRI GGCC 2 cut(s) 185, 600
BtsI GCAGTG 2 cut(s) 138, 180
BtsIMutI CAGTG 2 cut(s) 138, 180
Cac8I GCNNGC 3 cut(s) 64, 70, 237
CfoI GCGC 6 cut(s) 56, 68, 190, 201, 225, 235
Cfr10I RCCGGY 2 cut(s) 62, 68
Cfr13I GGNCC 3 cut(s) 206, 243, 598
CseI GACGC 2 cut(s) 61, 546
Csp6I GTAC 1 cut(s) 366
CviAII CATG 4 cut(s) 191, 451, 485, 639
CviJI RGCY 5 cut(s) 185, 387, 559, 600, 611
CviKI_1 RGCY 5 cut(s) 185, 387, 559, 600, 611
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 2 cut(s) 26, 278
DinI GGCGCC 2 cut(s) 55, 67
DpnI GATC 2 cut(s) 275, 653
DpnII GATC 2 cut(s) 273, 651
EaeI YGGCCR 1 cut(s) 183
Ecl136II GAGCTC 1 cut(s) 559
Eco24I GRGCYC 1 cut(s) 561
Eco47I GGWCC 2 cut(s) 206, 243
Eco53kI GAGCTC 1 cut(s) 559
Eco72I CACGTG 2 cut(s) 181, 618
Eco81I CCTNAGG 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 373
EcoICRI GAGCTC 1 cut(s) 559
EcoRII CCWGG 2 cut(s) 594, 600
EcoT22I ATGCAT 1 cut(s) 456
EcoT38I GRGCYC 1 cut(s) 561
EgeI GGCGCC 2 cut(s) 55, 67
EheI GGCGCC 2 cut(s) 55, 67
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 4 cut(s) 194, 454, 488, 642
FatI CATG 4 cut(s) 190, 450, 484, 638
Fnu4HI GCNGC 1 cut(s) 186
FriOI GRGCYC 1 cut(s) 561
Fsp4HI GCNGC 1 cut(s) 186
GlaI GCGC 6 cut(s) 55, 67, 189, 200, 224, 234
GluI GCNGC 1 cut(s) 186
HaeII RGCGCY 2 cut(s) 57, 69
HaeIII GGCC 2 cut(s) 185, 600
HapII CCGG 5 cut(s) 51, 63, 69, 80, 576
HgaI GACGC 2 cut(s) 61, 546
HhaI GCGC 6 cut(s) 56, 68, 190, 201, 225, 235
Hin1I GRCGYC 3 cut(s) 54, 66, 72
Hin1II CATG 4 cut(s) 194, 454, 488, 642
Hin6I GCGC 6 cut(s) 54, 66, 188, 199, 223, 233
HinP1I GCGC 6 cut(s) 54, 66, 188, 199, 223, 233
HinfI GANTC 1 cut(s) 344
HpaII CCGG 5 cut(s) 51, 63, 69, 80, 576
HphI GGTGA 2 cut(s) 353, 619
Hpy166II GTNNAC 6 cut(s) 136, 178, 368, 472, 536, 627
Hpy188I TCNGA 1 cut(s) 355
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 6 cut(s) 136, 178, 368, 472, 536, 627
Hpy99I CGWCG 1 cut(s) 62
HpyAV CCTTC 3 cut(s) 9, 405, 622
HpyCH4IV ACGT 4 cut(s) 180, 295, 315, 617
HpyCH4V TGCA 5 cut(s) 173, 178, 454, 484, 570
HpyF10VI GCNNNNNNNGC 3 cut(s) 196, 537, 639
HpyF3I CTNAG 2 cut(s) 26, 278
HpySE526I ACGT 4 cut(s) 180, 295, 315, 617
Hsp92I GRCGYC 3 cut(s) 54, 66, 72
Hsp92II CATG 4 cut(s) 194, 454, 488, 642
HspAI GCGC 6 cut(s) 54, 66, 188, 199, 223, 233
KasI GGCGCC 2 cut(s) 53, 65
KroI GCCGGC 2 cut(s) 62, 68
KroNI GCCGGC 2 cut(s) 64, 70
Kzo9I GATC 2 cut(s) 273, 651
LmnI GCTCC 1 cut(s) 592
LweI GCATC 3 cut(s) 150, 463, 485
MaeII ACGT 4 cut(s) 180, 295, 315, 617
MaeIII GTNAC 2 cut(s) 291, 322
MalI GATC 2 cut(s) 275, 653
MboI GATC 2 cut(s) 273, 651
MboII GAAGA 1 cut(s) 97
MflI RGATCY 1 cut(s) 651
MhlI GDGCHC 4 cut(s) 180, 561, 589, 608
MluCI AATT 6 cut(s) 36, 250, 308, 400, 409, 419
Mly113I GGCGCC 2 cut(s) 54, 66
MnlI CCTC 3 cut(s) 273, 349, 369
Mph1103I ATGCAT 1 cut(s) 456
MreI CGCCGGCG 2 cut(s) 62, 68
MroNI GCCGGC 2 cut(s) 62, 68
MseI TTAA 5 cut(s) 105, 111, 155, 312, 422
MslI CAYNNNNRTG 2 cut(s) 462, 620
MspI CCGG 5 cut(s) 51, 63, 69, 80, 576
MspR9I CCNGG 3 cut(s) 576, 596, 602
MvaI CCWGG 2 cut(s) 596, 602
MvnI CGCG 5 cut(s) 188, 201, 225, 235, 334
MwoI GCNNNNNNNGC 3 cut(s) 196, 537, 639
NaeI GCCGGC 2 cut(s) 64, 70
NarI GGCGCC 2 cut(s) 54, 66
NciI CCSGG 1 cut(s) 576
NdeII GATC 2 cut(s) 273, 651
NgoMIV GCCGGC 2 cut(s) 62, 68
NlaIII CATG 4 cut(s) 194, 454, 488, 642
NlaIV GGNNCC 3 cut(s) 55, 67, 653
NmuCI GTSAC 1 cut(s) 291
NsiI ATGCAT 1 cut(s) 456
NspI RCATGY 2 cut(s) 488, 642
PaeR7I CTCGAG 1 cut(s) 373
PdiI GCCGGC 2 cut(s) 64, 70
PfeI GAWTC 1 cut(s) 344
PkrI GCNGC 1 cut(s) 187
PluTI GGCGCC 2 cut(s) 57, 69
PmaCI CACGTG 2 cut(s) 181, 618
PmlI CACGTG 2 cut(s) 181, 618
Ppu21I YACGTR 2 cut(s) 181, 618
Psp124BI GAGCTC 1 cut(s) 561
Psp1406I AACGTT 1 cut(s) 315
Psp6I CCWGG 2 cut(s) 594, 600
PspCI CACGTG 2 cut(s) 181, 618
PspGI CCWGG 2 cut(s) 594, 600
PspN4I GGNNCC 3 cut(s) 55, 67, 653
PspPI GGNCC 3 cut(s) 206, 243, 598
PspXI VCTCGAGB 1 cut(s) 373
PsuI RGATCY 1 cut(s) 651
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
RseI CAYNNNNRTG 2 cut(s) 462, 620
SacI GAGCTC 1 cut(s) 561
SaqAI TTAA 5 cut(s) 105, 111, 155, 312, 422
SatI GCNGC 1 cut(s) 186
Sau3AI GATC 2 cut(s) 273, 651
Sau96I GGNCC 3 cut(s) 206, 243, 598
ScrFI CCNGG 3 cut(s) 576, 596, 602
SduI GDGCHC 4 cut(s) 180, 561, 589, 608
SfaNI GCATC 3 cut(s) 150, 463, 485
SfoI GGCGCC 2 cut(s) 55, 67
Sfr274I CTCGAG 1 cut(s) 373
SgrAI CRCCGGYG 2 cut(s) 62, 68
SinI GGWCC 2 cut(s) 206, 243
SlaI CTCGAG 1 cut(s) 373
SmiMI CAYNNNNRTG 2 cut(s) 462, 620
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 6 cut(s) 36, 250, 308, 400, 409, 419
SsiI CCGC 1 cut(s) 186
SspDI GGCGCC 2 cut(s) 53, 65
SspI AATATT 3 cut(s) 98, 120, 164
SstI GAGCTC 1 cut(s) 561
StyD4I CCNGG 3 cut(s) 574, 594, 600
TaiI ACGT 4 cut(s) 183, 298, 318, 620
TaqI TCGA 1 cut(s) 374
TasI AATT 6 cut(s) 36, 250, 308, 400, 409, 419
TauI GCSGC 1 cut(s) 188
TfiI GAWTC 1 cut(s) 344
Tru1I TTAA 5 cut(s) 105, 111, 155, 312, 422
Tru9I TTAA 5 cut(s) 105, 111, 155, 312, 422
TscAI CASTG 2 cut(s) 138, 180
TseFI GTSAC 1 cut(s) 291
Tsp45I GTSAC 1 cut(s) 291
TspGWI ACGGA 4 cut(s) 91, 129, 173, 198
TspRI CASTG 2 cut(s) 138, 180
VneI GTGCAC 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 206, 243
XapI RAATTY 1 cut(s) 250
XceI RCATGY 2 cut(s) 488, 642
XhoI CTCGAG 1 cut(s) 373
Zsp2I ATGCAT 1 cut(s) 456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.