pycom520g00370

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_520
OG0000513
Physical Location & Seq
Forward (+)
320996 .. 321618
623 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom520g00370.6

Sequence Viewer

Length: 381 bp
ATGGTATATCCATATGTATATTACTTGCATATAATTTGGGAATGCTTTCAACATGACTACAGTTATGTGTTAGGCAACTTTGATATTGTCGTACTGCTCATAAACCTGCACCCCGGTGTCAGTGCTCCCGCCAGTGGCCAGGGCACAGTCCTTCACGTGATGTTCACCTCCCGCACCTTACACTCACCTTGGATCCAAGGTAGATGCACAGTAACCCCGTATTTATTTACACCCAGTCTTGTCGTACATACCACTTTAGGTGGTTCCAACTCGTGTGCAGGTATACTTATTGAGGCTTGTTCTCGTGCATTTACGTTATGTTTCTTTAGTTATTTTAGTCCTTTAAACTATTTTCGTGTGTTTGTAGGTCCAAAGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

127

Amino Acids

14.14

Weight (kDa)

7.69

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 114, 269
AccI GTMKAC 1 cut(s) 283
AciI CCGC 2 cut(s) 129, 172
AclWI GGATC 2 cut(s) 187, 200
AcoI YGGCCR 1 cut(s) 136
AcvI CACGTG 1 cut(s) 157
AfaI GTAC 2 cut(s) 93, 246
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 1 cut(s) 50
AjnI CCWGG 1 cut(s) 138
AleI CACNNNNGTG 1 cut(s) 114
Alw21I GWGCWC 1 cut(s) 127
AlwI GGATC 2 cut(s) 187, 200
AoxI GGCC 1 cut(s) 136
Asp700I GAANNNNTTC 1 cut(s) 45
AspS9I GGNCC 1 cut(s) 368
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 157, 177
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 1 cut(s) 146
BalI TGGCCA 1 cut(s) 138
BamHI GGATCC 1 cut(s) 192
BauI CACGAG 2 cut(s) 271, 303
BbrPI CACGTG 1 cut(s) 157
Bbv12I GWGCWC 1 cut(s) 127
BciT130I CCWGG 1 cut(s) 140
BcnI CCSGG 1 cut(s) 114
BfmI CTRYAG 1 cut(s) 58
BfuAI ACCTGC 2 cut(s) 114, 269
Bme1390I CCNGG 2 cut(s) 114, 140
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 1 cut(s) 368
BmiI GGNNCC 2 cut(s) 194, 265
BmrFI CCNGG 2 cut(s) 114, 140
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 1 cut(s) 194
BmuI ACTGGG 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 114
BsaAI YACGTR 1 cut(s) 157
BsaJI CCNNGG 4 cut(s) 112, 139, 188, 196
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 132, 234
BseBI CCWGG 1 cut(s) 140
BseDI CCNNGG 4 cut(s) 112, 139, 188, 196
BseLI CCNNNNNNNGG 1 cut(s) 134
BseNI ACTGG 2 cut(s) 132, 234
BseSI GKGCMC 1 cut(s) 146
BsgI GTGCAG 2 cut(s) 92, 297
BshFI GGCC 1 cut(s) 138
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 1 cut(s) 114
BslI CCNNNNNNNGG 1 cut(s) 134
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 1 cut(s) 138
Bsp1286I GDGCHC 2 cut(s) 127, 146
Bsp143I GATC 1 cut(s) 192
BspACI CCGC 2 cut(s) 129, 172
BspANI GGCC 1 cut(s) 138
BspLI GGNNCC 2 cut(s) 194, 265
BspMI ACCTGC 2 cut(s) 114, 269
BspPI GGATC 2 cut(s) 187, 200
BsrI ACTGG 2 cut(s) 132, 234
BssECI CCNNGG 4 cut(s) 112, 139, 188, 196
BssMI GATC 1 cut(s) 192
BssNAI GTATAC 1 cut(s) 284
BssSI CACGAG 2 cut(s) 271, 303
BssT1I CCWWGG 2 cut(s) 188, 196
Bst1107I GTATAC 1 cut(s) 284
Bst2BI CACGAG 2 cut(s) 271, 303
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 3 cut(s) 62, 148, 211
BstBAI YACGTR 1 cut(s) 157
BstKTI GATC 1 cut(s) 195
BstMBI GATC 1 cut(s) 192
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 2 cut(s) 112, 138
BstSFI CTRYAG 1 cut(s) 58
BstSLI GKGCMC 1 cut(s) 146
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BstZ17I GTATAC 1 cut(s) 284
BsuRI GGCC 1 cut(s) 138
BtsIMutI CAGTG 2 cut(s) 127, 139
BveI ACCTGC 2 cut(s) 114, 269
Cfr13I GGNCC 1 cut(s) 368
Csp6I GTAC 2 cut(s) 92, 245
CviAII CATG 1 cut(s) 53
CviJI RGCY 3 cut(s) 138, 296, 378
CviKI_1 RGCY 3 cut(s) 138, 296, 378
CviQI GTAC 2 cut(s) 92, 245
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
DraI TTTAAA 1 cut(s) 345
EaeI YGGCCR 1 cut(s) 136
Eco130I CCWWGG 2 cut(s) 188, 196
Eco47I GGWCC 1 cut(s) 368
Eco72I CACGTG 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 188, 196
ErhI CCWWGG 2 cut(s) 188, 196
FaeI CATG 1 cut(s) 56
FatI CATG 1 cut(s) 52
FauI CCCGC 2 cut(s) 136, 179
FauNDI CATATG 1 cut(s) 13
FblI GTMKAC 1 cut(s) 283
HaeIII GGCC 1 cut(s) 138
HapII CCGG 1 cut(s) 114
Hin1II CATG 1 cut(s) 56
HpaII CCGG 1 cut(s) 114
HphI GGTGA 2 cut(s) 157, 177
Hpy166II GTNNAC 2 cut(s) 165, 284
Hpy8I GTNNAC 2 cut(s) 165, 284
HpyAV CCTTC 1 cut(s) 161
HpyCH4III ACNGT 3 cut(s) 62, 148, 211
HpyCH4IV ACGT 2 cut(s) 156, 314
HpyCH4V TGCA 5 cut(s) 28, 109, 207, 278, 308
HpySE526I ACGT 2 cut(s) 156, 314
Hsp92II CATG 1 cut(s) 56
Kzo9I GATC 1 cut(s) 192
LmnI GCTCC 1 cut(s) 130
LpnPI CCDG 7 cut(s) 119, 125, 127, 145, 152, 247, 264
LweI GCATC 1 cut(s) 194
MaeII ACGT 2 cut(s) 156, 314
MaeIII GTNAC 1 cut(s) 211
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 2 cut(s) 127, 146
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 1 cut(s) 33
MluNI TGGCCA 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 291
MnlI CCTC 2 cut(s) 178, 286
Mox20I TGGCCA 1 cut(s) 138
MroXI GAANNNNTTC 1 cut(s) 45
MscI TGGCCA 1 cut(s) 138
MseI TTAA 1 cut(s) 344
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 138
MspI CCGG 1 cut(s) 114
MspR9I CCNGG 2 cut(s) 114, 140
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 140
NciI CCSGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 13
NdeII GATC 1 cut(s) 192
NlaIII CATG 1 cut(s) 56
NlaIV GGNNCC 2 cut(s) 194, 265
OliI CACNNNNGTG 1 cut(s) 114
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 45
PmaCI CACGTG 1 cut(s) 157
PmlI CACGTG 1 cut(s) 157
Ppu21I YACGTR 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 138
PspCI CACGTG 1 cut(s) 157
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 2 cut(s) 194, 265
PspPI GGNCC 1 cut(s) 368
PsuI RGATCY 1 cut(s) 192
RsaI GTAC 2 cut(s) 93, 246
RsaNI GTAC 2 cut(s) 92, 245
RseI CAYNNNNRTG 1 cut(s) 114
SaqAI TTAA 1 cut(s) 344
Sau3AI GATC 1 cut(s) 192
Sau96I GGNCC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 114, 140
SduI GDGCHC 2 cut(s) 127, 146
SfaNI GCATC 1 cut(s) 194
SfcI CTRYAG 1 cut(s) 58
SinI GGWCC 1 cut(s) 368
SmiMI CAYNNNNRTG 1 cut(s) 114
Sse9I AATT 1 cut(s) 33
SsiI CCGC 2 cut(s) 129, 172
StyD4I CCNGG 2 cut(s) 112, 138
StyI CCWWGG 2 cut(s) 188, 196
TaaI ACNGT 3 cut(s) 62, 148, 211
TaiI ACGT 2 cut(s) 159, 317
TasI AATT 1 cut(s) 33
Tru1I TTAA 1 cut(s) 344
Tru9I TTAA 1 cut(s) 344
TscAI CASTG 2 cut(s) 127, 139
TspRI CASTG 2 cut(s) 127, 139
VpaK11BI GGWCC 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 283
XmnI GAANNNNTTC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.