AT4G30150

Nucleolar 27S pre-rRNA processing, Urb2 Npa2 (InterPro IPR018849)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000376
Physical Location & Seq
Forward (+)
14742292 .. 14750042
7751 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G30150.2

Sequence Viewer

Length: 6030 bp
ATGTTGAAAAGGAAGCTTACGAGTCCGAAGAAATCTAAGCCGTCGAAGAAGACGAAGAAGAACTCTAAAACCCAATTCGATGATTCTCCAGTAGAGGTCGTAGAAACCAAAGCTGAAGAGCTTCCGCGTACGGGTTCGAAGAAATCGAATCCGTCGAAGAAGAGAAAGCAGACGAAGAAGAATTCTGAAACTCAATTTGAAGATTCTTCAGTAGAGGTCGTAGAAACCAAAGCTTGTGACCAGGAGGAAACGGTTACGGACATTGTTGTTGAAGAGGGTCCATGGAAGAACCTTGAACTCATACTTTCGTTGCAGAGCAATACATTGGGCTTTAAAAAGAAGGTGGAGTTGGCTTTTAGTTTTGTTAAAGGTTATGGAGGAGAAAATGGGACTAATGAGGATGAAGAATGTCAAGCGGTTAAGATATCGCGGCTGATTATATTTCTCAGCGACTGGATCCAGTCTCTTTTGATTCCTTCAGAGAAGAATATTAAAGTTAAGTGTGATTTAGATTCTGAACCATGTTTGGACTTCAGGTGTTGGGAGATCTTCAGTTTCTGCTTGAAGGAGGCGACAATCTTGGGAGTCTCTTTAAATTTGTCAAGAAACCTATTAAAGGCTATTGGGCTTATCACGGGAAGATTTTTATCTGCCCTGAACGAATCCTTGGCTACGGGGGTTGATTTCTGCAATGGTCAAGGTTTTGTAGTTTACAGTTCTGTGGTTGATTGTCTCGGCTTGTTATTTTCATCGAAAAGTGGCATGTCTAATGACAATTTAGACTTGTGGTTTTCGACTGTTGAACCAGTTCTGAAGCTCACACATACGGTTCTTGTAGAGAATATCAAGGATAGTCTTGGTGACAGACATGTCCTTAAATTCTCTTGCTTGGTTCTTGAGCCATTCTCTAGGTTCTTAATGACTCATCCAACTACGAAGAATGGGTTTTGTGATTTTCTGGACAAGCTTTTTGAGCCATTTATGGATGTGTTGGGTTTATTAAATCTCATCGAGGACAAAAACAAGGATTTGGAGATATCCTTGCTGAGGTTGATTGAAGACATATTATCTCTGGCTCTGTTTCATTCAGCTCATATTGATGGGTTCTTAGGCCTGGGTGGAGCGAAAAAGTATTTACCAGAATCGAAAGAGAACAAAACTATTCTGAAAAGCTATCATCGGCATTTTTTTACCAAGTTCAAAAATATGCTATTAATGAAGAAGGAGTTGGAGCTGAGCTGTATGGGGTCATTGTTTAAAGTGTTTATTTACAGAGTGATGAAACAACAAAGAGATCCTAATCAGTTGCAGGAAGGCATGATGACAAAGGCCTCAAATGCGAGGCAAGCAGAAGAAAGGCCATGGAAGCTGGCGGACACAGCTACAAATGATAATGGGTCTTCTACAAAAAGTCATTACTCAAGTTCCCTCCGGCTGGAGACACGAAAATCAATTTTTGATTTCTTCCTGCATCTTATGGAACCTATATTACTTGAGATCAATGGATATAATCAGTCTGGCTCTGAAATGGCTCCTTTATTGGGTGATTTTTGTTGTGTTATTAAGTCAGCAAATAGTTTGCTGTTCAACTTTGCTCATGAGCGAATGTATGTGAAGACAGAAGATGCATCTGAAGGAGCTTGCTCTTGTTTTTTGAGGACGATCTTCAAAACAATAGTTTCAGTTGCTTCTGAATTAAAGAAACATTGTCCATATGATAATGGGTCAGAGATGCATGTTTTGTTAGCCAAGGAGCTAGTAACCGCAATAGGCTATCTGTTACATATTGAATACGAAATCATTGAGAGTGATTTAGTTACTTTGTGGCTAATCATTCTCTCTTTCCTGGAGTTTAGTAGTTTGTCACCAGAGAATTCAGAAGGGGACTGCCCGTTGACTTCATTGTTAGTTGGCCTTGGATGCCAGCTGATAACTTTATATAGTGACCTTCGCCAGGTAAGTGTTGCTGTATTTTCTCTGTTCAAAGCTGTAAGGCTTGTGATGCCTGTTGTGACACCTGCTGATGGTGATGATGATGAGATGATTGCCACTGAAGAGCTTCCACTATCAACTGTGTTCCCTTTCCGTTTAGAGAGAAGTGAAAAATCAGTGGAAAAGCTCTTGTCATCTCAAGCTTTAAGACTTGCTATACATAAAGCTATTAAAGTGATACCAGAAGGCCAAGCAAGTGGTTGTATCAAGAGCTTGACTGCAGATGTATCAAAAACCATGAAATGGATAAAACAAGTTTGTTGCTCAACAGGTGCTACAGAACAAGATGGACAGGTGGCAGCATTCTTGGCTGGATCTTTGTCTGATATTTATTCACTAATTCTCGACTCAATAACTATTACAACAGGGAATAGTAACCTTGTTGGCCAGTCCATGAAAGATCTGTTGGATCTCATCAGTCCTTGCTTAACCCACCTGGTTTCTTCAGACTCCGATTGCATCGAAAATTTCCTTTCTGCTCTCACCGGAAAAGATTTGGAGATTGTGATGGCTGAAAAGAAGATAGAGACCTACAGAAAGTCTGTGCGCTTGTTTGTTATATTCGTCTTGCGTATTTACATGTCTTCCAGAAGCTTATATAGGCAGGTGATTAGTCTTATGCCTCCGAAAAAAACAAAGGATATGGCTGGTATTAAGGGCGACTCTGTTGCAGTTCGTTGCGGAAGCGATTGGATAAAAGAGAAAAGTTGGAATTATGAAGGATATTTTTCATGGATCTCCCAACCTTCTGCTTCCATTGTTGACACTATTAAACACATTTCAGCTTTTTACCTTAAGGATGACAGTGCAGATTGCTCCTTGCTGATATATATATTGTATGGAGTCGCTCTTCAGAGACTTGTTGATTTAAACAGCCACATAAAATCACTTGATTATGTGTCACAGATAAGTGATAATCAGATAAATGATACAATGCTCAAGCATGTATCAGTTCTTAAGCGTGAAGGAGAAGAGCTTACTGATTTCCTTTTAGGCAACAACATCATATCAGGTTTTGTTGATGATGGAACATTTGAGACGATAAAAGACACTGATCAGTGGGTTCTTAGGGTTTCTGGCATCAACGGGAAGTGTTTGCCTACTATGCGTTTGTGGGTTCTTAGCCAGCATATTGATCTTTGGTGCCCCCATGCAGGAAAAAAGAAATTGAAGAATTTCCTGTCTCAGCTAATAGGTAGTTCCGTTCCTTGTATATTGAACGGAGTGGGTATGTCTACTCTTGGCTGGGAAAATAATGTAGACAAGGGCTCTCAAAAGAAGAAAATAGGGTTGGAACAGTTCTCGTTTGGACTTCTATTTGATTCAGTACTGTATGAACACGAATTTGTTCGCAGGTATTTGGCCCCGAGTTTTTCTCACGTACTGAAAATGACAGCAGAAACGTTTTTCAAGGATATTACTGAAGAAGTAAACTTTGATTCTCCATCAGATTGGTCAGAGGTGTTAATTTTGCTTGAAAGTTCAATTGCTAACTTATCTGGAAAGCTTAAATCAGAAGCCTTTCTAGAGGCACATGTGTCACTGCTGGACAATCGGAAGTTCACAGCTTGCCAAAATTTGCTAAATCTGTTAGGTGTGATGCCCAAGGAGTATACGAATAAGAAGTCGTTCCAACTTTATGCAAGTTATGTTCTCGACCTTGAGAGGTTTATAGTTTTTAGCATGTTGAGATGTTTGAACAAGCTGTCCTGTGGTGATATGCAAAACCTTTTCAGCCTTTTCAGTACTTGCAGAAAAACTTTGAAAAGTATTGCTATGATTTCTTGCGACAAGGTACTAGGAGCTACCAAGTTGCCTTTATCTGATAGTTCGTTGTTGGCTTCTTGGCTTTTTAAATCAGCACAAGCTGCGACTTGTCAAGTGAGATTTAGGAATGATGTTACAGGAAAAGCAAGGGACGCCCTCTTTTCTTTGATGGATCACACATCGTATATGTTTCTAACCGTAAGTAAATATCAATTTAGCAAGGCACTACCATTTTCTGATGAAAAACTCATTTCATCAGAAATTTCTGAGGGAACTGGACAAGCGAACCTTATAATTGAGAACTTGACAGAACAGGCAGAAACTCTACTAAATGCTTTGAGGGCTACCTTCAGAGATGAGAAAACAGCCTTTAAATGTGAGAGTCTGATACTGAACAAGTTAACACCTATATTTTCTTGTTTTAGTGGGTTGTTGTGGGGATTAGCATCTGCAGTGAGTAACAGAGATATGCAGAAGAATCATCAGAACGCAAAACTGAGATGGAAATCAGAACAGTTCTCAAAGCTTTCCCGCATTATCCATGTGCTTAGCAATTTTTTCGAGGTTTTTGCACAGTGTCTGTTTCTTAGTGGTGATGTGCAGCGAGAAATCCAAACCAATATCAACTGGACCAGATTGCTTGATGGGACCGAGGGTTCAAATGGTCTTGTGTGTGGTGACGTAGTAGAGACTAGTGATGTGAAAAAGAAAATTATTGAGAGCTTGATAAAGGGTGATTCTTCAGAAGTAGTATTGGCACTCAAGCATCTGTTAATTGCTTCTGCTGCTATTTTAAGGTTAAACCTGCAAATTGATGGCATTACATTCTCACCTACATTTGTCTCTGTTCTCACGAACATTTCGAATGATTTACTGTCTGTATTCGCTGACATGAGCGAGGCACCACTTGAATTCTCATTTATTTGGTTGGATGGTGCAGTTAAAGTTGTAGAAGAACTAGGGAGCCAATTCTGCTTATCTAACCCTACATTAAACATAGATCTGTACTCAAAGTTGATTGAGTTGCACCTGAAGGTGATTGGGAAATGCATATCACTACAAGGAAAAGAGGCTACCTTGGAATCTCATGAGACAGGATTTGGTACTAATGCAATCCATGCTAAGCTAGTGCTAACAGAAAAAAAACGGTCTCACAGATTGCATTGGTTGGACGAATTAAAACAGAGGCTTAGGATGTCATTTAAAGTGTTTATACATAGTTCCTCAGAGCTACACTTATTGTCAGGAGTACAAGCTATAGAAAGAGCACTGGTTGGAGTATGGGAAGTGTGCCCAGCTATCTATTGCATACAGACTGGAAATAGAGATGGAGGCAGAATTTCTGAAACCGTCGCTGCTGGTCTTGACTGTCTGGACCTAATTCTGGAACATGCCACAGGTCGCAAACGTTTGAATGTGGTTAAAAGGCACATTCAAGGCTTAATGTCTGCAGTGTTTGGTATAATGGCTCACATGCAGAGTCCATTTATTTTCTTCTCAAATGCAGTCGTTGGCAACCAGGGTTCCAATTCTCCAGATTCTGGGGCAGTCATTCTCATGTGTGTTGGAGTCTTGATAAGAATAGCAGGGAAACACGCTTTGTTTCGGATGGATTCATCCCACGTAAGTCAATCCATACATATACCCGGAGCAATTTTTCTTGATTACCTCCACGCAACAAGGGTGGGATTCTCGGTTTTGGATGGAAATCTGTTATCTAAGGATGATCAGCAACAAGATCTTTTAGGATGCTCAAAAGAACTTCAAGTAGACAGGAAATTCTCAGTGAGCCTGTATGCTGCTTGCTGCCGACTACTATATACAGCTGTTAAGCATCATAAGAGTCAAACTGAGGGGTCCATTGCTACACTCCAAGAATCTGTTTCCGCGCTTCTTCATTGTCTGGAGACAGCAGGAAAAAATTTGGGTAATTGTGTTTCATGGGAAGTGGAAGAGGGAATTAGATGTGCTTGTTTCCTCCGGAGGATCTATGAAGAGCTAAGACAGCAGAAGGAGGTCTTTGGACAGCATTGTTTCAAGTTCTTGTCAACTTACATATGGGTTTCTTCCGGATATGGACCTCTTAAGACGGGTCTTGAAAGGGAGGTAGATGAAGCTTTAAGGCCTGGTGTGTATGCTCTTATAGACTCTTGCTCACCTAACGATCTTCAATATCTCCATACAGTATTTGGTGAAGGTCCTTGTAGAAACTCCCTAGCAACTCTGCAACAAGACTACAAGTTGAATTTCAAGTATGGAGGAAAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

2009

Amino Acids

224.59

Weight (kDa)

6.53

Isoelectric Point (pI)

41.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1733 - 1968 8e-37 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4052
AarI CACCTGC 2 cut(s) 2026, 2587
AasI GACNNNNNNGTC 1 cut(s) 869
Acc36I ACCTGC 4 cut(s) 2026, 2587, 3336, 4572
AccB1I GGYRCC 2 cut(s) 3135, 4660
AccB7I CCANNNNNTGG 2 cut(s) 2235, 5300
AccI GTMKAC 4 cut(s) 3227, 3252, 3605, 5529
AccII CGCG 3 cut(s) 127, 430, 5648
AccIII TCCGGA 2 cut(s) 5739, 5828
AciI CCGC 8 cut(s) 125, 416, 430, 1373, 1764, 2673, 4291, 5646
AclI AACGTT 2 cut(s) 3395, 5167
AclWI GGATC 8 cut(s) 451, 464, 1289, 2314, 2409, 2735, 3939, 5753
AcoI YGGCCR 1 cut(s) 2377
AcyI GRCGYC 1 cut(s) 3912
AfaI GTAC 8 cut(s) 130, 3321, 3375, 3739, 3789, 4766, 4864, 5010
AflII CTTAAG 3 cut(s) 2786, 2948, 5843
AflIII ACRYGT 3 cut(s) 868, 2571, 3526
AhlI ACTAGT 1 cut(s) 4451
AjnI CCWGG 7 cut(s) 240, 1113, 1845, 1953, 2427, 5277, 5884
AloI GAACNNNNNNTCC 4 cut(s) 3683, 3715, 4399, 4431
Alw21I GWGCWC 1 cut(s) 5029
AlwI GGATC 8 cut(s) 451, 464, 1289, 2314, 2409, 2735, 3939, 5753
AlwNI CAGNNNCTG 4 cut(s) 453, 558, 4339, 5300
Ama87I CYCGRG 1 cut(s) 3358
Aor13HI TCCGGA 2 cut(s) 5739, 5828
AoxI GGCC 8 cut(s) 1111, 1329, 1358, 1912, 2179, 2377, 3354, 5882
ApeKI GCWGC 7 cut(s) 2291, 3860, 4360, 4544, 5114, 5558, 5565
ArsI GACNNNNNNTTYG 4 cut(s) 953, 985, 3602, 3634
AseI ATTAAT 1 cut(s) 1214
Asp700I GAANNNNTTC 7 cut(s) 120, 807, 2058, 3167, 3339, 3513, 3620
AspLEI GCGC 2 cut(s) 2541, 5650
AspS9I GGNCC 8 cut(s) 278, 3355, 4389, 4407, 5134, 5616, 5837, 5957
AsuC2I CCSGG 1 cut(s) 5406
AsuII TTCGAA 2 cut(s) 137, 4622
AvaI CYCGRG 1 cut(s) 3358
AvaII GGWCC 7 cut(s) 278, 4389, 4407, 5134, 5616, 5837, 5957
BaeGI GKGCMC 2 cut(s) 3140, 5054
BalI TGGCCA 1 cut(s) 2379
BamHI GGATCC 1 cut(s) 456
BanI GGYRCC 2 cut(s) 3135, 4660
BanII GRGCYC 1 cut(s) 3263
BbsI GAAGAC 5 cut(s) 56, 1065, 1392, 1622, 2568
Bbv12I GWGCWC 1 cut(s) 5029
BbvCI CCTCAGC 1 cut(s) 1046
BbvI GCAGC 7 cut(s) 2303, 3847, 4372, 4531, 5101, 5545, 5552
BceAI ACGGC 1 cut(s) 25
BcgI CGANNNNNNTGC 6 cut(s) 2642, 2676, 4300, 4310, 4334, 4344
BciT130I CCWGG 7 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5886
BclI TGATCA 2 cut(s) 3046, 5485
BcnI CCSGG 1 cut(s) 5406
BcuI ACTAGT 1 cut(s) 4451
BfaI CTAG 9 cut(s) 909, 1757, 3518, 3791, 4452, 4718, 4886, 5975, 6028
BfmI CTRYAG 6 cut(s) 2211, 2268, 2524, 4209, 5016, 5208
BfrI CTTAAG 3 cut(s) 2786, 2948, 5843
BfuAI ACCTGC 4 cut(s) 2026, 2587, 3336, 4572
BglII AGATCT 4 cut(s) 546, 2392, 4759, 5497
BisI GCNGC 8 cut(s) 431, 2292, 3861, 4361, 4545, 5115, 5559, 5566
BlpI GCTNAGC 3 cut(s) 1235, 4307, 4881
BlsI GCNGC 8 cut(s) 432, 2293, 3862, 4362, 4546, 5116, 5560, 5567
BmcAI AGTACT 2 cut(s) 3321, 3739
Bme1390I CCNGG 8 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5406, 5886
Bme18I GGWCC 7 cut(s) 278, 4389, 4407, 5134, 5616, 5837, 5957
BmeT110I CYCGRG 1 cut(s) 3358
BmgT120I GGNCC 8 cut(s) 278, 3355, 4389, 4407, 5134, 5616, 5837, 5957
BmrFI CCNGG 8 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5406, 5886
BpiI GAAGAC 5 cut(s) 56, 1065, 1392, 1622, 2568
BplI GAGNNNNNCTC 4 cut(s) 890, 922, 3352, 3384
BpmI CTGGAG 5 cut(s) 72, 1457, 1868, 5277, 5684
Bpu10I CCTNAGC 2 cut(s) 1046, 4949
Bpu1102I GCTNAGC 3 cut(s) 1235, 4307, 4881
Bpu14I TTCGAA 2 cut(s) 137, 4622
BpuEI CTTGAG 7 cut(s) 917, 1405, 1514, 2115, 2915, 3674, 4505
BpuMI CCSGG 1 cut(s) 5406
BsaAI YACGTR 2 cut(s) 3373, 5383
BsaBI GATNNNNATC 5 cut(s) 646, 1299, 4204, 4264, 5466
BsaHI GRCGYC 1 cut(s) 3912
BsaI GGTCTC 2 cut(s) 2513, 4914
BsaWI WCCGGW 3 cut(s) 2477, 5739, 5828
BsaXI ACNNNNNCTCC 1 cut(s) 6009
Bse1I ACTGG 9 cut(s) 89, 458, 460, 806, 2380, 4039, 4391, 5034, 5080
Bse3DI GCAATG 2 cut(s) 697, 5619
Bse8I GATNNNNATC 5 cut(s) 646, 1299, 4204, 4264, 5466
BseAI TCCGGA 2 cut(s) 5739, 5828
BseBI CCWGG 7 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5886
BseJI GATNNNNATC 5 cut(s) 646, 1299, 4204, 4264, 5466
BseMI GCAATG 2 cut(s) 697, 5619
BseMII CTCAG 9 cut(s) 460, 1037, 1226, 3191, 4017, 4247, 4998, 5556, 5601
BseNI ACTGG 9 cut(s) 89, 458, 460, 806, 2380, 4039, 4391, 5034, 5080
BseRI GAGGAG 1 cut(s) 393
BseSI GKGCMC 2 cut(s) 3140, 5054
BseXI GCAGC 7 cut(s) 2303, 3847, 4372, 4531, 5101, 5545, 5552
BseYI CCCAGC 2 cut(s) 3237, 5053
BsgI GTGCAG 3 cut(s) 2820, 4379, 4716
Bsh1236I CGCG 3 cut(s) 127, 430, 5648
BshFI GGCC 8 cut(s) 1113, 1331, 1360, 1914, 2181, 2379, 3356, 5884
BshNI GGYRCC 2 cut(s) 3135, 4660
BsiHKAI GWGCWC 1 cut(s) 5029
BsiHKCI CYCGRG 1 cut(s) 3358
BsiSI CCGG 5 cut(s) 1432, 2478, 5406, 5740, 5829
BsiWI CGTACG 1 cut(s) 128
BslFI GGGAC 4 cut(s) 403, 1898, 3923, 4420
BsmBI CGTCTC 1 cut(s) 3023
BsmFI GGGAC 4 cut(s) 403, 1898, 3923, 4420
BsmI GAATGC 1 cut(s) 2294
BsnI GGCC 8 cut(s) 1113, 1331, 1360, 1914, 2181, 2379, 3356, 5884
Bso31I GGTCTC 2 cut(s) 2513, 4914
BsoBI CYCGRG 1 cut(s) 3358
Bsp119I TTCGAA 2 cut(s) 137, 4622
Bsp1286I GDGCHC 4 cut(s) 3140, 3263, 5029, 5054
Bsp13I TCCGGA 2 cut(s) 5739, 5828
Bsp1720I GCTNAGC 3 cut(s) 1235, 4307, 4881
Bsp19I CCATGG 2 cut(s) 281, 1361
BspACI CCGC 8 cut(s) 125, 416, 430, 1373, 1764, 2673, 4291, 5646
BspANI GGCC 8 cut(s) 1113, 1331, 1360, 1914, 2181, 2379, 3356, 5884
BspCNI CTCAG 9 cut(s) 459, 1038, 1227, 3190, 4018, 4248, 4997, 5555, 5602
BspEI TCCGGA 2 cut(s) 5739, 5828
BspFNI CGCG 3 cut(s) 127, 430, 5648
BspHI TCATGA 2 cut(s) 1597, 4846
BspMAI CTGCAG 3 cut(s) 2215, 4213, 5212
BspMI ACCTGC 4 cut(s) 2026, 2587, 3336, 4572
BspPI GGATC 8 cut(s) 451, 464, 1289, 2314, 2409, 2735, 3939, 5753
BspQI GCTCTTC 5 cut(s) 111, 2049, 2847, 2958, 5748
BspT104I TTCGAA 2 cut(s) 137, 4622
BspT107I GGYRCC 2 cut(s) 3135, 4660
BspTI CTTAAG 3 cut(s) 2786, 2948, 5843
BspTNI GGTCTC 2 cut(s) 2513, 4914
BsrDI GCAATG 2 cut(s) 697, 5619
BsrI ACTGG 9 cut(s) 89, 458, 460, 806, 2380, 4039, 4391, 5034, 5080
BssNAI GTATAC 1 cut(s) 3606
BssNI GRCGYC 1 cut(s) 3912
BssT1I CCWWGG 7 cut(s) 281, 666, 1361, 1749, 1915, 3597, 4836
Bst1107I GTATAC 1 cut(s) 3606
Bst2UI CCWGG 7 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5886
Bst6I CTCTTC 8 cut(s) 111, 155, 267, 2049, 2847, 2958, 5706, 5748
BstACI GRCGYC 1 cut(s) 3912
BstAFI CTTAAG 3 cut(s) 2786, 2948, 5843
BstAPI GCANNNNNTGC 2 cut(s) 3860, 4877
BstBAI YACGTR 2 cut(s) 3373, 5383
BstBI TTCGAA 2 cut(s) 137, 4622
BstC8I GCNNGC 7 cut(s) 1347, 1371, 1642, 1925, 3119, 3562, 5563
BstDSI CCRYGG 2 cut(s) 281, 1361
BstENI CCTNNNNNAGG 3 cut(s) 614, 1045, 1952
BstFNI CGCG 3 cut(s) 127, 430, 5648
BstHHI GCGC 2 cut(s) 2541, 5650
BstNI CCWGG 7 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5886
BstNSI RCATGY 9 cut(s) 766, 872, 1739, 2575, 2939, 3530, 3679, 5153, 5236
BstSCI CCNGG 8 cut(s) 240, 1113, 1845, 1953, 2427, 5277, 5404, 5884
BstSFI CTRYAG 6 cut(s) 2211, 2268, 2524, 4209, 5016, 5208
BstSLI GKGCMC 2 cut(s) 3140, 5054
BstUI CGCG 3 cut(s) 127, 430, 5648
BstV1I GCAGC 7 cut(s) 2303, 3847, 4372, 4531, 5101, 5545, 5552
BstV2I GAAGAC 5 cut(s) 56, 1065, 1392, 1622, 2568
BstXI CCANNNNNNTGG 2 cut(s) 2189, 3444
BstZ17I GTATAC 1 cut(s) 3606
BsuRI GGCC 8 cut(s) 1113, 1331, 1360, 1914, 2181, 2379, 3356, 5884
BtgI CCRYGG 2 cut(s) 281, 1361
BtsI GCAGTG 3 cut(s) 3533, 4218, 5217
BveI ACCTGC 4 cut(s) 2026, 2587, 3336, 4572
Cac8I GCNNGC 7 cut(s) 1347, 1371, 1642, 1925, 3119, 3562, 5563
CaiI CAGNNNCTG 4 cut(s) 453, 558, 4339, 5300
CciI TCATGA 2 cut(s) 1597, 4846
CfoI GCGC 2 cut(s) 2541, 5650
Cfr13I GGNCC 8 cut(s) 278, 3355, 4389, 4407, 5134, 5616, 5837, 5957
CseI GACGC 1 cut(s) 3920
CsiI ACCWGGT 1 cut(s) 2427
Csp6I GTAC 8 cut(s) 129, 3320, 3374, 3738, 3788, 4765, 4863, 5009
CspCI CAANNNNNGTGG 2 cut(s) 5424, 5459
CviQI GTAC 8 cut(s) 129, 3320, 3374, 3738, 3788, 4765, 4863, 5009
DraI TTTAAA 7 cut(s) 334, 594, 1258, 2862, 3847, 4132, 4963
DrdI GACNNNNNNGTC 1 cut(s) 869
DseDI GACNNNNNNGTC 1 cut(s) 869
EaeI YGGCCR 1 cut(s) 2377
Eam1104I CTCTTC 8 cut(s) 111, 155, 267, 2049, 2847, 2958, 5706, 5748
EarI CTCTTC 8 cut(s) 111, 155, 267, 2049, 2847, 2958, 5706, 5748
EciI GGCGGA 1 cut(s) 1388
Eco130I CCWWGG 7 cut(s) 281, 666, 1361, 1749, 1915, 3597, 4836
Eco147I AGGCCT 3 cut(s) 1113, 1331, 5884
Eco24I GRGCYC 1 cut(s) 3263
Eco31I GGTCTC 2 cut(s) 2513, 4914
Eco32I GATATC 2 cut(s) 426, 1038
Eco47I GGWCC 7 cut(s) 278, 4389, 4407, 5134, 5616, 5837, 5957
Eco88I CYCGRG 1 cut(s) 3358
EcoNI CCTNNNNNAGG 3 cut(s) 614, 1045, 1952
EcoO109I RGGNCCY 1 cut(s) 5957
EcoRI GAATTC 3 cut(s) 181, 1873, 4670
EcoRII CCWGG 7 cut(s) 240, 1113, 1845, 1953, 2427, 5277, 5884
EcoRV GATATC 2 cut(s) 426, 1038
EcoT14I CCWWGG 7 cut(s) 281, 666, 1361, 1749, 1915, 3597, 4836
EcoT22I ATGCAT 3 cut(s) 1630, 1737, 4811
EcoT38I GRGCYC 1 cut(s) 3263
ErhI CCWWGG 7 cut(s) 281, 666, 1361, 1749, 1915, 3597, 4836
Esp3I CGTCTC 1 cut(s) 3023
FalI AAGNNNNNCTT 4 cut(s) 4032, 4064, 5762, 5794
FaqI GGGAC 4 cut(s) 403, 1898, 3923, 4420
FauI CCCGC 1 cut(s) 4298
FauNDI CATATG 2 cut(s) 1714, 5816
FbaI TGATCA 2 cut(s) 3046, 5485
FblI GTMKAC 4 cut(s) 3227, 3252, 3605, 5529
Fnu4HI GCNGC 8 cut(s) 431, 2292, 3861, 4361, 4545, 5115, 5559, 5566
FriOI GRGCYC 1 cut(s) 3263
Fsp4HI GCNGC 8 cut(s) 431, 2292, 3861, 4361, 4545, 5115, 5559, 5566
FspBI CTAG 9 cut(s) 909, 1757, 3518, 3791, 4452, 4718, 4886, 5975, 6028
GlaI GCGC 2 cut(s) 2540, 5649
GluI GCNGC 8 cut(s) 431, 2292, 3861, 4361, 4545, 5115, 5559, 5566
GsaI CCCAGC 2 cut(s) 3241, 5057
GsuI CTGGAG 5 cut(s) 72, 1457, 1868, 5277, 5684
HaeIII GGCC 8 cut(s) 1113, 1331, 1360, 1914, 2181, 2379, 3356, 5884
HapII CCGG 5 cut(s) 1432, 2478, 5406, 5740, 5829
HgaI GACGC 1 cut(s) 3920
HhaI GCGC 2 cut(s) 2541, 5650
Hin1I GRCGYC 1 cut(s) 3912
Hin6I GCGC 2 cut(s) 2539, 5648
HinP1I GCGC 2 cut(s) 2539, 5648
HincII GTYRAC 4 cut(s) 1896, 2755, 4161, 5808
HindII GTYRAC 4 cut(s) 1896, 2755, 4161, 5808
HindIII AAGCTT 8 cut(s) 14, 231, 965, 2133, 2584, 3497, 4283, 5874
HpaI GTTAAC 1 cut(s) 4161
HpaII CCGG 5 cut(s) 1432, 2478, 5406, 5740, 5829
Hpy99I CGWCG 3 cut(s) 46, 157, 5114
HpyCH4IV ACGT 5 cut(s) 3372, 3395, 4440, 5167, 5382
HpySE526I ACGT 5 cut(s) 3372, 3395, 4440, 5167, 5382
Hsp92I GRCGYC 1 cut(s) 3912
HspAI GCGC 2 cut(s) 2539, 5648
Kpn2I TCCGGA 2 cut(s) 5739, 5828
Ksp22I TGATCA 2 cut(s) 3046, 5485
KspAI GTTAAC 1 cut(s) 4161
LguI GCTCTTC 5 cut(s) 111, 2049, 2847, 2958, 5748
LmnI GCTCC 9 cut(s) 1121, 1231, 1537, 1637, 1753, 2813, 3794, 4722, 5408
Lsp1109I GCAGC 7 cut(s) 2303, 3847, 4372, 4531, 5101, 5545, 5552
MabI ACCWGGT 1 cut(s) 2427
MaeI CTAG 9 cut(s) 909, 1757, 3518, 3791, 4452, 4718, 4886, 5975, 6028
MaeII ACGT 5 cut(s) 3372, 3395, 4440, 5167, 5382
MfeI CAATTG 1 cut(s) 3477
MhlI GDGCHC 4 cut(s) 3140, 3263, 5029, 5054
MlsI TGGCCA 1 cut(s) 2379
MluNI TGGCCA 1 cut(s) 2379
Mox20I TGGCCA 1 cut(s) 2379
Mph1103I ATGCAT 3 cut(s) 1630, 1737, 4811
MroI TCCGGA 2 cut(s) 5739, 5828
MroXI GAANNNNTTC 7 cut(s) 120, 807, 2058, 3167, 3339, 3513, 3620
MscI TGGCCA 1 cut(s) 2379
MslI CAYNNNNRTG 2 cut(s) 1098, 5315
Msp20I TGGCCA 1 cut(s) 2379
MspA1I CMGCKG 2 cut(s) 1927, 5585
MspCI CTTAAG 3 cut(s) 2786, 2948, 5843
MspI CCGG 5 cut(s) 1432, 2478, 5406, 5740, 5829
MspR9I CCNGG 8 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5406, 5886
MunI CAATTG 1 cut(s) 3477
Mva1269I GAATGC 1 cut(s) 2294
MvaI CCWGG 7 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5886
MvnI CGCG 3 cut(s) 127, 430, 5648
NciI CCSGG 1 cut(s) 5406
NcoI CCATGG 2 cut(s) 281, 1361
NdeI CATATG 2 cut(s) 1714, 5816
NmeAIII GCCGAG 1 cut(s) 714
NmuCI GTSAC 8 cut(s) 236, 860, 1863, 1943, 2011, 2892, 3531, 4436
NsiI ATGCAT 3 cut(s) 1630, 1737, 4811
NspI RCATGY 9 cut(s) 766, 872, 1739, 2575, 2939, 3530, 3679, 5153, 5236
NspV TTCGAA 2 cut(s) 137, 4622
PagI TCATGA 2 cut(s) 1597, 4846
PaqCI CACCTGC 2 cut(s) 2026, 2587
PceI AGGCCT 3 cut(s) 1113, 1331, 5884
PciI ACATGT 3 cut(s) 868, 2571, 3526
PciSI GCTCTTC 5 cut(s) 111, 2049, 2847, 2958, 5748
PcsI WCGNNNNNNNCGW 4 cut(s) 50, 143, 152, 4619
PctI GAATGC 1 cut(s) 2294
PdmI GAANNNNTTC 7 cut(s) 120, 807, 2058, 3167, 3339, 3513, 3620
Pfl23II CGTACG 1 cut(s) 128
PflMI CCANNNNNTGG 2 cut(s) 2235, 5300
PfoI TCCNGGA 1 cut(s) 1845
PkrI GCNGC 8 cut(s) 432, 2293, 3862, 4362, 4546, 5116, 5560, 5567
Ppu21I YACGTR 2 cut(s) 3373, 5383
PpuMI RGGWCCY 1 cut(s) 5957
PscI ACATGT 3 cut(s) 868, 2571, 3526
PshBI ATTAAT 1 cut(s) 1214
PsiI TTATAA 1 cut(s) 4052
Psp1406I AACGTT 2 cut(s) 3395, 5167
Psp5II RGGWCCY 1 cut(s) 5957
Psp6I CCWGG 7 cut(s) 240, 1113, 1845, 1953, 2427, 5277, 5884
PspFI CCCAGC 2 cut(s) 3237, 5053
PspGI CCWGG 7 cut(s) 240, 1113, 1845, 1953, 2427, 5277, 5884
PspLI CGTACG 1 cut(s) 128
PspPI GGNCC 8 cut(s) 278, 3355, 4389, 4407, 5134, 5616, 5837, 5957
PspPPI RGGWCCY 1 cut(s) 5957
PsrI GAACNNNNNNTAC 2 cut(s) 1473, 1505
PstI CTGCAG 3 cut(s) 2215, 4213, 5212
PstNI CAGNNNCTG 4 cut(s) 453, 558, 4339, 5300
PvuII CAGCTG 2 cut(s) 1927, 5585
RsaI GTAC 8 cut(s) 130, 3321, 3375, 3739, 3789, 4766, 4864, 5010
RsaNI GTAC 8 cut(s) 129, 3320, 3374, 3738, 3788, 4765, 4863, 5009
RseI CAYNNNNRTG 2 cut(s) 1098, 5315
SapI GCTCTTC 5 cut(s) 111, 2049, 2847, 2958, 5748
SatI GCNGC 8 cut(s) 431, 2292, 3861, 4361, 4545, 5115, 5559, 5566
Sau96I GGNCC 8 cut(s) 278, 3355, 4389, 4407, 5134, 5616, 5837, 5957
ScaI AGTACT 2 cut(s) 3321, 3739
ScrFI CCNGG 8 cut(s) 242, 1115, 1847, 1955, 2429, 5279, 5406, 5886
SduI GDGCHC 4 cut(s) 3140, 3263, 5029, 5054
SexAI ACCWGGT 1 cut(s) 2427
SfcI CTRYAG 6 cut(s) 2211, 2268, 2524, 4209, 5016, 5208
SfuI TTCGAA 2 cut(s) 137, 4622
SinI GGWCC 7 cut(s) 278, 4389, 4407, 5134, 5616, 5837, 5957
SmiMI CAYNNNNRTG 2 cut(s) 1098, 5315
SpeI ACTAGT 1 cut(s) 4451
SseBI AGGCCT 3 cut(s) 1113, 1331, 5884
SsiI CCGC 8 cut(s) 125, 416, 430, 1373, 1764, 2673, 4291, 5646
SspI AATATT 1 cut(s) 490
SspMI CTAG 9 cut(s) 909, 1757, 3518, 3791, 4452, 4718, 4886, 5975, 6028
StuI AGGCCT 3 cut(s) 1113, 1331, 5884
StyD4I CCNGG 8 cut(s) 240, 1113, 1845, 1953, 2427, 5277, 5404, 5884
StyI CCWWGG 7 cut(s) 281, 666, 1361, 1749, 1915, 3597, 4836
TaiI ACGT 5 cut(s) 3375, 3398, 4443, 5170, 5385
TaqII GACCGA 1 cut(s) 4424
TatI WGTACW 4 cut(s) 3319, 3737, 4764, 5008
TauI GCSGC 1 cut(s) 433
TseFI GTSAC 8 cut(s) 236, 860, 1863, 1943, 2011, 2892, 3531, 4436
TseI GCWGC 7 cut(s) 2291, 3860, 4360, 4544, 5114, 5558, 5565
Tsp45I GTSAC 8 cut(s) 236, 860, 1863, 1943, 2011, 2892, 3531, 4436
TspGWI ACGGA 5 cut(s) 141, 272, 2075, 3184, 3228
Van91I CCANNNNNTGG 2 cut(s) 2235, 5300
Vha464I CTTAAG 3 cut(s) 2786, 2948, 5843
VpaK11BI GGWCC 7 cut(s) 278, 4389, 4407, 5134, 5616, 5837, 5957
VspI ATTAAT 1 cut(s) 1214
XagI CCTNNNNNAGG 3 cut(s) 614, 1045, 1952
XbaI TCTAGA 1 cut(s) 3517
XceI RCATGY 9 cut(s) 766, 872, 1739, 2575, 2939, 3530, 3679, 5153, 5236
XcmI CCANNNNNNNNNTGG 1 cut(s) 5945
XmiI GTMKAC 4 cut(s) 3227, 3252, 3605, 5529
XmnI GAANNNNTTC 7 cut(s) 120, 807, 2058, 3167, 3339, 3513, 3620
XspI CTAG 9 cut(s) 909, 1757, 3518, 3791, 4452, 4718, 4886, 5975, 6028
ZrmI AGTACT 2 cut(s) 3321, 3739
Zsp2I ATGCAT 3 cut(s) 1630, 1737, 4811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.