Urb2/Npa2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 69 |
| AciI | CCGC | 3 cut(s) | 69, 92, 106 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AgsI | TTSAA | 2 cut(s) | 155, 335 |
| AjnI | CCWGG | 1 cut(s) | 201 |
| AluBI | AGCT | 3 cut(s) | 17, 167, 235 |
| AluI | AGCT | 3 cut(s) | 17, 167, 235 |
| Alw26I | GTCTC | 1 cut(s) | 25 |
| AoxI | GGCC | 2 cut(s) | 3, 89 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 134, 166 |
| AspS9I | GGNCC | 2 cut(s) | 71, 188 |
| AsuHPI | GGTGA | 1 cut(s) | 108 |
| AvaII | GGWCC | 2 cut(s) | 71, 188 |
| BciT130I | CCWGG | 1 cut(s) | 203 |
| BcoDI | GTCTC | 1 cut(s) | 25 |
| BisI | GCNGC | 1 cut(s) | 92 |
| BlsI | GCNGC | 1 cut(s) | 93 |
| Bme1390I | CCNGG | 1 cut(s) | 203 |
| Bme18I | GGWCC | 2 cut(s) | 71, 188 |
| BmgT120I | GGNCC | 2 cut(s) | 71, 188 |
| BmiI | GGNNCC | 2 cut(s) | 73, 200 |
| BmrFI | CCNGG | 1 cut(s) | 203 |
| BpmI | CTGGAG | 2 cut(s) | 33, 224 |
| BsaJI | CCNNGG | 1 cut(s) | 191 |
| BseBI | CCWGG | 1 cut(s) | 203 |
| BseDI | CCNNGG | 1 cut(s) | 191 |
| BseGI | GGATG | 1 cut(s) | 266 |
| BshFI | GGCC | 2 cut(s) | 5, 91 |
| BsmAI | GTCTC | 1 cut(s) | 25 |
| BsnI | GGCC | 2 cut(s) | 5, 91 |
| Bsp143I | GATC | 1 cut(s) | 238 |
| Bsp19I | CCATGG | 1 cut(s) | 191 |
| BspACI | CCGC | 3 cut(s) | 69, 92, 106 |
| BspANI | GGCC | 2 cut(s) | 5, 91 |
| BspLI | GGNNCC | 2 cut(s) | 73, 200 |
| BspQI | GCTCTTC | 1 cut(s) | 265 |
| BsrBI | CCGCTC | 1 cut(s) | 69 |
| BssECI | CCNNGG | 1 cut(s) | 191 |
| BssMI | GATC | 1 cut(s) | 238 |
| BssT1I | CCWWGG | 1 cut(s) | 191 |
| Bst2UI | CCWGG | 1 cut(s) | 203 |
| Bst6I | CTCTTC | 2 cut(s) | 50, 265 |
| BstDSI | CCRYGG | 1 cut(s) | 191 |
| BstF5I | GGATG | 1 cut(s) | 266 |
| BstKTI | GATC | 1 cut(s) | 241 |
| BstMAI | GTCTC | 1 cut(s) | 25 |
| BstMBI | GATC | 1 cut(s) | 238 |
| BstNI | CCWGG | 1 cut(s) | 203 |
| BstSCI | CCNGG | 1 cut(s) | 201 |
| BsuRI | GGCC | 2 cut(s) | 5, 91 |
| BtgI | CCRYGG | 1 cut(s) | 191 |
| BtsCI | GGATG | 1 cut(s) | 266 |
| Cfr13I | GGNCC | 2 cut(s) | 71, 188 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 166, 201 |
| CviAII | CATG | 1 cut(s) | 192 |
| CviJI | RGCY | 5 cut(s) | 5, 17, 91, 167, 235 |
| CviKI_1 | RGCY | 5 cut(s) | 5, 17, 91, 167, 235 |
| DpnI | GATC | 1 cut(s) | 240 |
| DpnII | GATC | 1 cut(s) | 238 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 2 cut(s) | 50, 265 |
| EarI | CTCTTC | 2 cut(s) | 50, 265 |
| Eco130I | CCWWGG | 1 cut(s) | 191 |
| Eco47I | GGWCC | 2 cut(s) | 71, 188 |
| EcoRII | CCWGG | 1 cut(s) | 201 |
| EcoT14I | CCWWGG | 1 cut(s) | 191 |
| ErhI | CCWWGG | 1 cut(s) | 191 |
| FaeI | CATG | 1 cut(s) | 195 |
| FaiI | YATR | 3 cut(s) | 193, 256, 345 |
| FatI | CATG | 1 cut(s) | 191 |
| Fnu4HI | GCNGC | 1 cut(s) | 92 |
| FokI | GGATG | 1 cut(s) | 253 |
| Fsp4HI | GCNGC | 1 cut(s) | 92 |
| GluI | GCNGC | 1 cut(s) | 92 |
| GsuI | CTGGAG | 2 cut(s) | 33, 224 |
| HaeIII | GGCC | 2 cut(s) | 5, 91 |
| Hin1II | CATG | 1 cut(s) | 195 |
| HinfI | GANTC | 2 cut(s) | 8, 80 |
| HphI | GGTGA | 1 cut(s) | 108 |
| Hpy188I | TCNGA | 2 cut(s) | 85, 313 |
| Hpy188III | TCNNGA | 3 cut(s) | 12, 223, 362 |
| Hsp92II | CATG | 1 cut(s) | 195 |
| Kzo9I | GATC | 1 cut(s) | 238 |
| LguI | GCTCTTC | 1 cut(s) | 265 |
| LmnI | GCTCC | 1 cut(s) | 14 |
| LpnPI | CCDG | 4 cut(s) | 132, 188, 215, 236 |
| MalI | GATC | 1 cut(s) | 240 |
| MbiI | CCGCTC | 1 cut(s) | 69 |
| MboI | GATC | 1 cut(s) | 238 |
| MboII | GAAGA | 8 cut(s) | 55, 64, 67, 89, 233, 252, 255, 359 |
| MluCI | AATT | 4 cut(s) | 112, 210, 330, 336 |
| MmeI | TCCRAC | 1 cut(s) | 139 |
| MnlI | CCTC | 1 cut(s) | 189 |
| MseI | TTAA | 3 cut(s) | 209, 306, 329 |
| MspR9I | CCNGG | 1 cut(s) | 203 |
| MvaI | CCWGG | 1 cut(s) | 203 |
| NcoI | CCATGG | 1 cut(s) | 191 |
| NdeII | GATC | 1 cut(s) | 238 |
| NlaIII | CATG | 1 cut(s) | 195 |
| NlaIV | GGNNCC | 2 cut(s) | 73, 200 |
| PciSI | GCTCTTC | 1 cut(s) | 265 |
| PfeI | GAWTC | 2 cut(s) | 8, 80 |
| PkrI | GCNGC | 1 cut(s) | 93 |
| Psp6I | CCWGG | 1 cut(s) | 201 |
| PspGI | CCWGG | 1 cut(s) | 201 |
| PspN4I | GGNNCC | 2 cut(s) | 73, 200 |
| PspPI | GGNCC | 2 cut(s) | 71, 188 |
| SapI | GCTCTTC | 1 cut(s) | 265 |
| SaqAI | TTAA | 3 cut(s) | 209, 306, 329 |
| SatI | GCNGC | 1 cut(s) | 92 |
| Sau3AI | GATC | 1 cut(s) | 238 |
| Sau96I | GGNCC | 2 cut(s) | 71, 188 |
| ScrFI | CCNGG | 1 cut(s) | 203 |
| SetI | ASST | 5 cut(s) | 19, 169, 187, 204, 237 |
| SinI | GGWCC | 2 cut(s) | 71, 188 |
| Sse9I | AATT | 4 cut(s) | 112, 210, 330, 336 |
| SsiI | CCGC | 3 cut(s) | 69, 92, 106 |
| StyD4I | CCNGG | 1 cut(s) | 201 |
| StyI | CCWWGG | 1 cut(s) | 191 |
| TasI | AATT | 4 cut(s) | 112, 210, 330, 336 |
| TauI | GCSGC | 1 cut(s) | 94 |
| TfiI | GAWTC | 2 cut(s) | 8, 80 |
| Tru1I | TTAA | 3 cut(s) | 209, 306, 329 |
| Tru9I | TTAA | 3 cut(s) | 209, 306, 329 |
| TspDTI | ATGAA | 4 cut(s) | 147, 255, 287, 360 |
| VpaK11BI | GGWCC | 2 cut(s) | 71, 188 |