Rh6DG024200

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000376
Physical Location & Seq
Reverse (-)
2164203 .. 2194169
29967 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG024200.1

Sequence Viewer

Length: 6210 bp
ATGGCCGATTCTGGAGCTAACCCTAAACAGAGACAACCCAAGAAGAACAAGAAGAAGAGAAAGCAGAGCGGACCCGAAGAATCTGAAAGGCCGCCCAAAACTAACCGCACCAATTCATCAGAGAACGAGAATGAACCACAAGACCAAAACTTTGAAGTTGGAAGAGCTGAAAAGCCCAATCAAGGTGGTCCATGGAGGAACCTGGAGTTAATTCTGTCAATCCAGAACAGAGAGCTTGATCTTCACAAGAAGGTGGAACTAGCTTATGGTTTTGTGATGTCGAGAGGGAAGGAAGAAGGAGGCAGCAGTGATGAGGATAATCAAGCAGTGAACATGTCACGTTTAGTAATTTTTGTCAGTGATTGGATCCAGTCATTGTTGATTTCCGAAGGAAAGAAAGTTGAGAGTGGTGGAGAGAAGCATCAAGCTGAAGTTATTTATACGTATTTGGATTTAAGATGCTGGGAGATTTTCAAATTCTGTTTGGAGGAGTCTCTGAAATTAAATGTTTCTTTAACTTTCTCACGGAACCTTTTACGCTCTATATGTTGGATTGCAAGAAGTGCACTGTCCTCATTGAACAAAACATTTATTGGTGAAGGGTTACAATTGTACACTACTGTGCTTGATTGTATTTCTTTGGTATTTTCATCTCAAGAAGGCTTCTCAAATGAAAATTTAGACATGTGGATTTCAACTGTAAGTTCAGTTCTTGACCTTGGACACAAATTTTACAGTGAAGCTCTTGTTAGTGGCAATGAGGGTGTATTTGTTTTCCGATTCTTGTGCTTGGTGCTTGAGCCATTTGCCAGGTTTTTAAGGGCTCACGCAGCTCGGAAAGATGGATTTCGTGATTTTAGTAATAAGCTCCTTGAGCCTCTGTTGCATCTCTTGGGTCTCTTGCATCTCCAGATTGGTGGAAGTAATCCTGGTTGGACGAGAAACTTAGTGAAGCTTGTTGAAGATGTATTATCACATGGGCTATATCACCCACTCCACATTGATGGGTTTTTGAGTTTATGCAGTACTGAAAAATGCGCCACATCCAATCATGGGAAGTCAAAAGATTCCAAAACAATCAACCAGAGTTATCATAGACATTTATTTGATAAAGTGGAAGGAATTTTAGCTGCAAAGAATGCTTTTGCAGTAGAAAGCATAGGAGTATTGTTTCATTTGCTTATTGATCAAGTTAAAAAGCTAAAGAGGGATTCAGCGCTGACTGCAAGTACCAAAATGATGGGGAAAAGTGAAGGTTCAAGGCAGTTAGAAGATAGTTCATTGGGTCAAATGTCTACGATGTCGTCTGAGAGTCGTATTGTTGAAAAAAATTATTGCTCAACTAGTTTCAATCCAGAGACACGAAAGTCGCTTCACTATTTTTTTGTACTGATTATGGAGCCTCTTTTGCTTGAGATCAATGGCTTTCTTGAAACTAAGCTGAAAGTGGGCCCTGAATTGATGGATGTTCATTGCACACTTAAAGCTATAAATAATGTACTTTCGGTCTTTGTGCATGAAAAGATTTATGTGAGAACAGAGGATGCCTCTGAGGGAGCTTGTCTTAATTTCTTGAAGAAGGTTTATAATATCATCATCTCATTGTCCTCCGGTTTAATTCAGTTGTCAGTTACACACTCAGATAGTATTACTCTAATAGCAAATGAGGTACTTTCTGCTGTAGGCTATCTTTTGGAAATTGAATATGATGTTCTAGAGAATGATCTGGTAAGCTTGTGGTTAATGATGCTCTCTTACTTGGCCATTGGGCTTTCTTTGGTGGATTCTCCGGATAGATGCTCATTATTCTCCAAGATAACAGATACTGGATGCCAATTAATTATGCTCTATAGTCAACTTCGCCAGGTGAATAATACTATCTTTGCATTGTGTAAAGCAATAAGGGTTATTAATTTACATAATAGCGATGGTGAACTGAACTATACTAGATTTGTCATTCCTTTTCATGGTGAAGCATATGCAAAAGCTGTGGAAATACTATTATCTGCGCACGAATTCAAAATTGCAATCCATAAAGCAATGAAGTCCATACCAGAAGGACAAGCAAGTCAGTGTATTTGGCAGTTAACATTGGATATATCAGAATCTCTGGAGTGGATGAAAGTTAGTTGTTCGGAGGCTGATGAAAAAGAATTTTCTGAATGCCATCTAAGTAGCTTGCACAGTTTTAATCTGGAAGCTGAACTTTACGGGAGAGGGTTGTCAGAAATATATGCACTATTCCTAGAGTCATTGATTGTCACAACTGGAAACTGTAACCTTCTTGGCGCTTCTATAAAGGAATTAATTAGAGTGATTTTCCCTTGCATGAGCAATCTGGTTGGACCACAGCAGGAGGATGCTGTAAACAAATTCCTATGGTCTGTCGCGGTAAAAGATTCCGACAATGTGATCGCTAGAAACAAAAACAAATATCTGATATTTGGAGTCTCCACTTATTGGGTAGTTTTGTTCTTCTTTCGATTGTATAGCTCCTGCCGATGCTTATATAGGCAAGCAGCAATCCTTATGCCTCCAGATTTATCAAGAAAGATGTCTGTAGAAATGGGAGATTCGTTCTCCTCATTCTCTGGCAGGGATTGGATGGAAATGTCTGATTGGATCAATGGGGGATTTTTCTCTTGGATTGTACAACCTTCAGCTTCTCTTCTTTCTGTGATTCAATCTGTTTCAAACATCTTTTGTAAGGACCGTGCAGCTGATTCTTGTCCTTTAACTTATGTAATGCATGCTATGGCTTGTGAAAGGCTTGTGGATTTGAATAGTCGTATAAAGTCTTTTGAATATTTGATTGAAAATGGTGACAATCTGGTGCAAGTTGCGGAGATCTCTTCGTTGAGGCAAGAGGCAGCAGGACTCACTGGATTTATGATGGAGCACCTCTCATTAGTGTCTGAAGATCAACAGCGAATATTCATTTCTGCTGATACGACTAATAATAAGATGGTTGACTATGAAAGTGATGAATGGGATTTCAGTATTTGCTTTGTGAACAAGAAGTCATTGCCAACTGCACTTTGGTGGGTTGTTTGCCAAAATATTCATGCATGGTGCCCTCATGCCTCTAAAAAGGATTTGAAGAGATTCCTTTCTCTTTTAATCCATACTTCAATTCCCTATGTAAGAAGCAGCTTGGGGGTTATAGAGCACAAGAACCATGAAGCTGACAGGCTGAAGAATGTGGCATTGCATCAAATCTCATCACAGTGCTTTATTGATTCCAGTCTGTATGAGCAAAGATTTGTCCGCAGGTATTTTGCAAAGAGGTTCTGCCGTGCATTGGAGAAATCTACATTACCATTCATCAGCGACTTTCCATCCGGCAATGTTAAATTCAAGTCATCACCTAATTGGCCGAATGTCTTAAGTGATTTAGAGAACTCATCATTGGCTATTTCCTGCAACAAACTCAATGTATTTGATTGTTCCTCAGCCTCAAGTTGCAAGGGACAAAACTCCCAACCATCTACCATCATGAAATTTACAGCTTGTCAGAGTTTGATTAATCTTTTGAGTTGTATGCCAAAAGGTCATTTGGACACGAGATCATTTTCATGTTATGTGACTTCTATTCTGAACCTTGAAAGGATTGTTGTTGGCGGCTTATTAGATTATCAGAATGCTTCATACTCAACTTATTATTATGAGCTCTTCAGATTGTTTGTGTCCTGTCGGAAGGCCTTAAGATGTATAATTACTGCTTGTGAGGAGACAGTAGCCAGTCGAACCTCAGACACTGTACTCTTTGAGGATTTATTTCCAGTTTCTTGGCTTTACAAGTCAGTGCATATGGTTGCTGGGCTTCAAGAATCATTCTCAAAAGATATTTATCATCAAGTTCATGATATGATCTTGGCGTTAATGGATCACACATTTTATGTGTTTCTGACATTAAATAAATATCAATCCAATCATGCTGTTCGTTTCCTTGAGGTTGCTGAGCTGAATTTTGGATGTGCCCAAGAACAGAGAAGTTTATTGAATTCGTCCAATTATATTGAAGGCTGGAAAAGTGTAAACGTTGTTGCTAAGATTTTGAAGGAACAGATGCAGATCTTACTTGTAAATGTGAAGAATGGCATTTGTAATGGAAAAGAGGAAGTTTCTGTTGATGCTTTAAATTTGAACAAGTTTGCTTCCATAATTTCGTGTTTTAGTGGGTTTTTATGGGGCCTTGCATGCGTTGTGATCGACACAGATGGGAGAAATAGTGATGAGAAAGCTAAGTTGTCAAGGTGGAAACTTGAACCAATCTCCGAACTCAACCTCTGTATAAAGGTTTTTGAAGAAGTTTCTAGTCTTCTACTACAAATGTTTATTCATGACGATAATCAGCAGTCCACAACTAGATGTCATGCCTATAATCTTCAGAAGTCAGGCTACAATTTAGATTTGTTGGGTGCAGAGAAGATTTTGCCGGAAGGCAATGATGCTGACACTGATATGGCATGTGGTGGACTGCAGGATGAATCTGCAGTTGCAGTGACGTGCTCAGCATCATCTGACATCTGTGTTGACTCTGTAATTGGTAGTGTTCATAGAAGAAGACCGCGCTTGAAAGATGCAAATAGTGTTGTTAGTGTTCTCACTGCTGTTGATTCATTTGAACTGCAATCTTTAAATAAGCCTTTGTTGAGAAGCATGCTAAAAGGTGATTTCCCCAACGCAGCATTTTTACTCAGGCAGTTGTTAATCGCCTCTTCTGCTATTTTGAGGCTAAATTTGCATATTAAGAGCGCTCCCATGTTGTCAAGCTTGGTGCATAAGTTTGCTGGCATTATGCAAGTCTTGTTATTGGAATCAGCGGACGCAAGTCAGGTGCCACACGTCTATTATTTTGTGTGTTTAGATGGTGTGCTGAAGTATCTGGAAGAATTAGGGAATCATTTTCCTTTCACTAATCCTACCTTATCCAGAGATTTGTTTGACAAGATGGTTCAGCTACAGTTACGGGCTCTAGGGAAATGCATAACTTTACAAGGGAAAAGAGCAACTCTTGCATCTCATGAAACAAGCACCAACACACCCATAGGAGAAAGGAGATTTTCTGAAGCATCATCTTTTTCTGGCTGGGAATATTTATTGGATGAATTCAAAGCTAGGTTGAGGTCATCATTTGCAGTGTTCATAAAGAAATCAACAGAGTTGCATCTCCAATCTGCAGTTCAAGCTATCGAAAGAGCCCTAGTTGGTGTGCAGGAAGGGTGCACAGTGAGGTATGACATATGTGCTGGAAGTGAAGATGGAGGAAAGGTTTCTTCAATTGTTGCATCTGGCATTGATTGCTTGGATTTGGTTCTGGAATTTGTTTCAGGACGCAATTTGATTGTGGTTAAAAAATACATTCAGAGGTTAATTGCCTGCATGTTCAATGTTATTCTGCATTTGCAAAGTCCACTAATCTTCTATGAGAGATTCACTCAGAGTAAAGATGATAGTGATCCAGATCCAGGGACAGTCATTCTTATGTGTGTTGACGTACTGGCAAGAATTTCTGGCAAGCATGCAATATACAAAATGGATCTCTGGCATGTAGCACACTCGTTTCGTATACCTTCAGCACTTTTTCAAGATTTCCATCTGTTAAAACATTCTAAATGTCCTGTTCCAAATGATTCTTCAACAAGTCCAAATAACCAACCATCCGACCCAGTAGCAAGTGTACATGTCTCTGGTATAGATAGGCAATACTCAATCGGCCTATATTCTGGTTGCTGCCGATTATTGCATAATGTTGTGAAGCATCACAAAAGTGAATGCGAGGGGTACGTTGCTCTACTTCAAGCTTCTGTTCGTGTTCTTCTTTATTGTTTGGAGACATTGGATGCTGTTGTGGTAGCCAGGGAAGGCTTCTTTTCATGGGAAGTAGAAGAAGGAGTCAAATGTGCATGTTCTCTCAGAAGGATTTATGAAGAGCTAAGACATCAAAAAGAGGTCTTTGGTCCACACTGTTACCACTTTTTAGCTTATTACATACGGGTTTATTCAGGATATGGCCCCCGCAAAACAGGCATCAAAAGGGAAATAGATGAAGCTCTAAGACCAGGTGTATATGCGCTGATAGATGTTTGCTCACCTGATGATCTTCAACGTCTTCATACCTCATTTGGAGAGGGTCCTTGCAGAAACACTTTGGCAACCTTGAAGCACGATTACGAACTCAATTTCCAGTACCAGGGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

2069

Amino Acids

232.9

Weight (kDa)

6.38

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1830 - 2068 1.4e-37 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1587
AasI GACNNNNNNGTC 2 cut(s) 1366, 2067
Acc16I TGCGCA 1 cut(s) 2010
Acc36I ACCTGC 1 cut(s) 3261
AccB1I GGYRCC 2 cut(s) 3072, 4840
AccB7I CCANNNNNTGG 1 cut(s) 2460
AccBSI CCGCTC 1 cut(s) 69
AccI GTMKAC 2 cut(s) 1295, 5572
AccII CGCG 2 cut(s) 2390, 4573
AccIII TCCGGA 1 cut(s) 1789
AclI AACGTT 1 cut(s) 4041
AclWI GGATC 7 cut(s) 361, 374, 2630, 3894, 5456, 5462, 5550
AcoI YGGCCR 3 cut(s) 3, 1761, 3374
AcuI CTGAAG 9 cut(s) 450, 2643, 2937, 3215, 3658, 4373, 4901, 5091, 5562
AfeI AGCGCT 2 cut(s) 1218, 4759
AfiI CCNNNNNNNGG 7 cut(s) 182, 1053, 1967, 2460, 3301, 5471, 6196
AflII CTTAAG 2 cut(s) 3385, 3703
AflIII ACRYGT 4 cut(s) 333, 684, 4846, 5686
AhlI ACTAGT 1 cut(s) 1343
AjiI CACGTC 2 cut(s) 4509, 4849
AjnI CCWGG 8 cut(s) 201, 809, 928, 1863, 5470, 5861, 6064, 6195
AjuI GAANNNNNNNTTGG 6 cut(s) 3392, 3424, 5169, 5201, 5623, 5655
AleI CACNNNNGTG 3 cut(s) 620, 3040, 5772
AloI GAACNNNNNNTCC 2 cut(s) 6171, 6203
Alw21I GWGCWC 6 cut(s) 568, 2901, 3171, 3672, 4514, 5231
Alw26I GTCTC 8 cut(s) 25, 498, 902, 1352, 2455, 3725, 5695, 5831
Alw44I GTGCAC 2 cut(s) 564, 5227
AlwI GGATC 7 cut(s) 361, 374, 2630, 3894, 5456, 5462, 5550
AlwNI CAGNNNCTG 2 cut(s) 1826, 3758
Aor13HI TCCGGA 1 cut(s) 1789
Aor51HI AGCGCT 2 cut(s) 1218, 4759
AoxI GGCC 9 cut(s) 3, 89, 1450, 1761, 3374, 3699, 4192, 5719, 6016
ApaI GGGCCC 1 cut(s) 1454
ApaLI GTGCAC 2 cut(s) 564, 5227
ApeKI GCWGC 9 cut(s) 303, 830, 1130, 2519, 2717, 2870, 3150, 4688, 5736
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AseI ATTAAT 4 cut(s) 1838, 1911, 2306, 3525
Asp700I GAANNNNTTC 2 cut(s) 3104, 5275
AspLEI GCGC 7 cut(s) 1040, 1219, 2011, 2291, 4575, 4760, 6079
AsuHPI GGTGA 9 cut(s) 608, 980, 1879, 1943, 1982, 2834, 3357, 4685, 6087
AvaII GGWCC 6 cut(s) 71, 188, 2345, 2710, 5963, 6137
BaeGI GKGCMC 5 cut(s) 568, 1454, 3077, 3982, 5231
BalI TGGCCA 1 cut(s) 1763
BamHI GGATCC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 3072, 4840
BanII GRGCYC 5 cut(s) 826, 1454, 3672, 4978, 5206
BarI GAAGNNNNNNTAC 4 cut(s) 4385, 4417, 6158, 6190
BauI CACGAG 1 cut(s) 3562
BbsI GAAGAC 3 cut(s) 4313, 4573, 6107
Bbv12I GWGCWC 6 cut(s) 568, 2901, 3171, 3672, 4514, 5231
BbvCI CCTCAGC 1 cut(s) 3451
BbvI GCAGC 9 cut(s) 315, 842, 1117, 2531, 2729, 2882, 3162, 4700, 5723
BceAI ACGGC 1 cut(s) 3279
BcgI CGANNNNNNTGC 2 cut(s) 768, 802
BciT130I CCWGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
BclI TGATCA 1 cut(s) 1186
BcoDI GTCTC 8 cut(s) 25, 498, 902, 1352, 2455, 3725, 5695, 5831
BcuI ACTAGT 1 cut(s) 1343
BfmI CTRYAG 7 cut(s) 1680, 1849, 2559, 4481, 4494, 4964, 5181
BfoI RGCGCY 3 cut(s) 1220, 2292, 4761
BfrI CTTAAG 2 cut(s) 3385, 3703
BfuAI ACCTGC 1 cut(s) 3261
BglII AGATCT 2 cut(s) 2847, 4074
BlpI GCTNAGC 2 cut(s) 3960, 4513
BmcAI AGTACT 1 cut(s) 1027
Bme1390I CCNGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
Bme18I GGWCC 6 cut(s) 71, 188, 2345, 2710, 5963, 6137
BmgBI CACGTC 2 cut(s) 4509, 4849
BmrFI CCNGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
BmrI ACTGGG 1 cut(s) 5666
BmuI ACTGGG 1 cut(s) 5666
BoxI GACNNNNGTC 1 cut(s) 4833
BpiI GAAGAC 3 cut(s) 4313, 4573, 6107
BplI GAGNNNNNCTC 6 cut(s) 1532, 1564, 2888, 2920, 5425, 5457
BpmI CTGGAG 5 cut(s) 33, 224, 893, 2132, 2520
Bpu10I CCTNAGC 1 cut(s) 3451
Bpu1102I GCTNAGC 2 cut(s) 3960, 4513
BpuEI CTTGAG 6 cut(s) 639, 818, 893, 1433, 3442, 3971
BsaAI YACGTR 1 cut(s) 444
BsaBI GATNNNNATC 5 cut(s) 3849, 4073, 5460, 5466, 5598
BsaI GGTCTC 1 cut(s) 902
BsaJI CCNNGG 5 cut(s) 191, 718, 5471, 5862, 6196
BsaWI WCCGGW 2 cut(s) 1610, 1789
BsaXI ACNNNNNCTCC 4 cut(s) 4216, 4246, 5259, 5289
Bsc4I CCNNNNNNNGG 7 cut(s) 182, 1053, 1967, 2460, 3301, 5471, 6196
Bse3DI GCAATG 7 cut(s) 763, 1471, 2046, 3023, 3206, 3352, 4453
Bse8I GATNNNNATC 5 cut(s) 3849, 4073, 5460, 5466, 5598
BseAI TCCGGA 1 cut(s) 1789
BseBI CCWGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
BseDI CCNNGG 5 cut(s) 191, 718, 5471, 5862, 6196
BseJI GATNNNNATC 5 cut(s) 3849, 4073, 5460, 5466, 5598
BseLI CCNNNNNNNGG 7 cut(s) 182, 1053, 1967, 2460, 3301, 5471, 6196
BseMI GCAATG 7 cut(s) 763, 1471, 2046, 3023, 3206, 3352, 4453
BseRI GAGGAG 3 cut(s) 503, 2572, 3743
BseSI GKGCMC 5 cut(s) 568, 1454, 3077, 3982, 5231
BseXI GCAGC 9 cut(s) 315, 842, 1117, 2531, 2729, 2882, 3162, 4700, 5723
BseYI CCCAGC 3 cut(s) 462, 3818, 5091
BsgI GTGCAG 4 cut(s) 2736, 3018, 4443, 5237
Bsh1236I CGCG 2 cut(s) 2390, 4573
BshFI GGCC 9 cut(s) 5, 91, 1452, 1763, 3376, 3701, 4194, 5721, 6018
BshNI GGYRCC 2 cut(s) 3072, 4840
BsiHKAI GWGCWC 6 cut(s) 568, 2901, 3171, 3672, 4514, 5231
BsiSI CCGG 4 cut(s) 1611, 1790, 3342, 4439
BslFI GGGAC 2 cut(s) 3483, 5488
BslI CCNNNNNNNGG 7 cut(s) 182, 1053, 1967, 2460, 3301, 5471, 6196
BsmAI GTCTC 8 cut(s) 25, 498, 902, 1352, 2455, 3725, 5695, 5831
BsmFI GGGAC 2 cut(s) 3483, 5488
BsmI GAATGC 4 cut(s) 1144, 2168, 3646, 5783
BsnI GGCC 9 cut(s) 5, 91, 1452, 1763, 3376, 3701, 4194, 5721, 6018
Bso31I GGTCTC 1 cut(s) 902
Bsp120I GGGCCC 1 cut(s) 1450
Bsp13I TCCGGA 1 cut(s) 1789
Bsp1407I TGTACA 3 cut(s) 612, 2650, 5683
Bsp1720I GCTNAGC 2 cut(s) 3960, 4513
Bsp19I CCATGG 1 cut(s) 191
BspANI GGCC 9 cut(s) 5, 91, 1452, 1763, 3376, 3701, 4194, 5721, 6018
BspEI TCCGGA 1 cut(s) 1789
BspFNI CGCG 2 cut(s) 2390, 4573
BspHI TCATGA 4 cut(s) 3495, 3862, 4342, 5026
BspMAI CTGCAG 3 cut(s) 4485, 4498, 5185
BspMI ACCTGC 1 cut(s) 3261
BspPI GGATC 7 cut(s) 361, 374, 2630, 3894, 5456, 5462, 5550
BspQI GCTCTTC 3 cut(s) 157, 3677, 5928
BspT107I GGYRCC 2 cut(s) 3072, 4840
BspTI CTTAAG 2 cut(s) 3385, 3703
BspTNI GGTCTC 1 cut(s) 902
BsrBI CCGCTC 1 cut(s) 69
BsrDI GCAATG 7 cut(s) 763, 1471, 2046, 3023, 3206, 3352, 4453
BsrGI TGTACA 3 cut(s) 612, 2650, 5683
BssECI CCNNGG 5 cut(s) 191, 718, 5471, 5862, 6196
BssNAI GTATAC 1 cut(s) 5573
BssSI CACGAG 1 cut(s) 3562
BssT1I CCWWGG 2 cut(s) 191, 718
Bst1107I GTATAC 1 cut(s) 5573
Bst2BI CACGAG 1 cut(s) 3562
Bst2UI CCWGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
Bst6I CTCTTC 8 cut(s) 50, 157, 2673, 2857, 3095, 3677, 4726, 5928
BstAFI CTTAAG 2 cut(s) 3385, 3703
BstAPI GCANNNNNTGC 4 cut(s) 563, 1139, 5018, 5304
BstAUI TGTACA 3 cut(s) 612, 2650, 5683
BstBAI YACGTR 1 cut(s) 444
BstC8I GCNNGC 9 cut(s) 2180, 2517, 2751, 4201, 4664, 4795, 5383, 5522, 5526
BstDSI CCRYGG 1 cut(s) 191
BstFNI CGCG 2 cut(s) 2390, 4573
BstH2I RGCGCY 3 cut(s) 1220, 2292, 4761
BstHHI GCGC 7 cut(s) 1040, 1219, 2011, 2291, 4575, 4760, 6079
BstMAI GTCTC 8 cut(s) 25, 498, 902, 1352, 2455, 3725, 5695, 5831
BstNI CCWGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
BstPAI GACNNNNGTC 1 cut(s) 4833
BstSCI CCNGG 8 cut(s) 201, 809, 928, 1863, 5470, 5861, 6064, 6195
BstSFI CTRYAG 7 cut(s) 1680, 1849, 2559, 4481, 4494, 4964, 5181
BstSLI GKGCMC 5 cut(s) 568, 1454, 3077, 3982, 5231
BstSNI TACGTA 1 cut(s) 444
BstUI CGCG 2 cut(s) 2390, 4573
BstV1I GCAGC 9 cut(s) 315, 842, 1117, 2531, 2729, 2882, 3162, 4700, 5723
BstV2I GAAGAC 3 cut(s) 4313, 4573, 6107
BstX2I RGATCY 5 cut(s) 366, 2847, 4074, 5467, 5542
BstXI CCANNNNNNTGG 4 cut(s) 917, 1004, 1240, 3789
BstYI RGATCY 5 cut(s) 366, 2847, 4074, 5467, 5542
BstZ17I GTATAC 1 cut(s) 5573
BsuRI GGCC 9 cut(s) 5, 91, 1452, 1763, 3376, 3701, 4194, 5721, 6018
BtgI CCRYGG 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 1941
BtrI CACGTC 2 cut(s) 4509, 4849
BtsI GCAGTG 5 cut(s) 313, 333, 4509, 4608, 5148
BveI ACCTGC 1 cut(s) 3261
Cac8I GCNNGC 9 cut(s) 2180, 2517, 2751, 4201, 4664, 4795, 5383, 5522, 5526
CaiI CAGNNNCTG 2 cut(s) 1826, 3758
CciI TCATGA 4 cut(s) 3495, 3862, 4342, 5026
CfoI GCGC 7 cut(s) 1040, 1219, 2011, 2291, 4575, 4760, 6079
CseI GACGC 2 cut(s) 4838, 5346
CsiI ACCWGGT 1 cut(s) 6064
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 1971, 2006
DraI TTTAAA 2 cut(s) 4140, 4641
DrdI GACNNNNNNGTC 2 cut(s) 1366, 2067
DseDI GACNNNNNNGTC 2 cut(s) 1366, 2067
EaeI YGGCCR 3 cut(s) 3, 1761, 3374
Eam1104I CTCTTC 8 cut(s) 50, 157, 2673, 2857, 3095, 3677, 4726, 5928
EarI CTCTTC 8 cut(s) 50, 157, 2673, 2857, 3095, 3677, 4726, 5928
Ecl136II GAGCTC 1 cut(s) 3670
Eco105I TACGTA 1 cut(s) 444
Eco130I CCWWGG 2 cut(s) 191, 718
Eco147I AGGCCT 1 cut(s) 3701
Eco24I GRGCYC 5 cut(s) 826, 1454, 3672, 4978, 5206
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 6 cut(s) 71, 188, 2345, 2710, 5963, 6137
Eco47III AGCGCT 2 cut(s) 1218, 4759
Eco53kI GAGCTC 1 cut(s) 3670
Eco57I CTGAAG 9 cut(s) 450, 2643, 2937, 3215, 3658, 4373, 4901, 5091, 5562
EcoICRI GAGCTC 1 cut(s) 3670
EcoO109I RGGNCCY 3 cut(s) 1451, 4192, 6137
EcoRI GAATTC 3 cut(s) 2015, 4003, 5111
EcoRII CCWGG 8 cut(s) 201, 809, 928, 1863, 5470, 5861, 6064, 6195
EcoT14I CCWWGG 2 cut(s) 191, 718
EcoT22I ATGCAT 3 cut(s) 2751, 3070, 4991
EcoT38I GRGCYC 5 cut(s) 826, 1454, 3672, 4978, 5206
ErhI CCWWGG 2 cut(s) 191, 718
FalI AAGNNNNNCTT 2 cut(s) 2190, 2222
FaqI GGGAC 2 cut(s) 3483, 5488
FauI CCCGC 1 cut(s) 6029
FauNDI CATATG 3 cut(s) 1978, 3810, 5246
FbaI TGATCA 1 cut(s) 1186
FblI GTMKAC 2 cut(s) 1295, 5572
FriOI GRGCYC 5 cut(s) 826, 1454, 3672, 4978, 5206
FspI TGCGCA 1 cut(s) 2010
GlaI GCGC 7 cut(s) 1039, 1218, 2010, 2290, 4574, 4759, 6078
GsaI CCCAGC 3 cut(s) 466, 3822, 5095
GsuI CTGGAG 5 cut(s) 33, 224, 893, 2132, 2520
HaeII RGCGCY 3 cut(s) 1220, 2292, 4761
HaeIII GGCC 9 cut(s) 5, 91, 1452, 1763, 3376, 3701, 4194, 5721, 6018
HapII CCGG 4 cut(s) 1611, 1790, 3342, 4439
HgaI GACGC 2 cut(s) 4838, 5346
HhaI GCGC 7 cut(s) 1040, 1219, 2011, 2291, 4575, 4760, 6079
Hin6I GCGC 7 cut(s) 1038, 1217, 2009, 2289, 4573, 4758, 6077
HinP1I GCGC 7 cut(s) 1038, 1217, 2009, 2289, 4573, 4758, 6077
HincII GTYRAC 5 cut(s) 1856, 2088, 2971, 4537, 5497
HindII GTYRAC 5 cut(s) 1856, 2088, 2971, 4537, 5497
HindIII AAGCTT 4 cut(s) 953, 1732, 4774, 5805
HpaI GTTAAC 1 cut(s) 2088
HpaII CCGG 4 cut(s) 1611, 1790, 3342, 4439
HphI GGTGA 9 cut(s) 608, 980, 1879, 1943, 1982, 2834, 3357, 4685, 6087
HpyCH4IV ACGT 8 cut(s) 340, 443, 4041, 4508, 4848, 5499, 5790, 6112
HpySE526I ACGT 8 cut(s) 340, 443, 4041, 4508, 4848, 5499, 5790, 6112
HspAI GCGC 7 cut(s) 1038, 1217, 2009, 2289, 4573, 4758, 6077
Kpn2I TCCGGA 1 cut(s) 1789
Ksp22I TGATCA 1 cut(s) 1186
KspAI GTTAAC 1 cut(s) 2088
LguI GCTCTTC 3 cut(s) 157, 3677, 5928
LmnI GCTCC 7 cut(s) 14, 873, 1399, 1556, 2498, 2896, 4765
Lsp1109I GCAGC 9 cut(s) 315, 842, 1117, 2531, 2729, 2882, 3162, 4700, 5723
MabI ACCWGGT 1 cut(s) 6064
MaeII ACGT 8 cut(s) 340, 443, 4041, 4508, 4848, 5499, 5790, 6112
MbiI CCGCTC 1 cut(s) 69
MfeI CAATTG 2 cut(s) 608, 5283
MflI RGATCY 5 cut(s) 366, 2847, 4074, 5467, 5542
MlsI TGGCCA 1 cut(s) 1763
MluNI TGGCCA 1 cut(s) 1763
MlyI GAGTC 7 cut(s) 500, 1321, 2258, 2457, 2871, 4532, 5907
MmeI TCCRAC 7 cut(s) 139, 530, 914, 2323, 2427, 3674, 5691
Mox20I TGGCCA 1 cut(s) 1763
Mph1103I ATGCAT 3 cut(s) 2751, 3070, 4991
MroI TCCGGA 1 cut(s) 1789
MroXI GAANNNNTTC 2 cut(s) 3104, 5275
MscI TGGCCA 1 cut(s) 1763
MslI CAYNNNNRTG 8 cut(s) 620, 1002, 3040, 3226, 3574, 4463, 5486, 5772
Msp20I TGGCCA 1 cut(s) 1763
MspA1I CMGCKG 2 cut(s) 2720, 4826
MspCI CTTAAG 2 cut(s) 3385, 3703
MspI CCGG 4 cut(s) 1611, 1790, 3342, 4439
MspR9I CCNGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
MunI CAATTG 2 cut(s) 608, 5283
Mva1269I GAATGC 4 cut(s) 1144, 2168, 3646, 5783
MvaI CCWGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
MvnI CGCG 2 cut(s) 2390, 4573
NcoI CCATGG 1 cut(s) 191
NdeI CATATG 3 cut(s) 1978, 3810, 5246
NmuCI GTSAC 5 cut(s) 336, 2260, 2822, 3583, 4504
NsbI TGCGCA 1 cut(s) 2010
NsiI ATGCAT 3 cut(s) 2751, 3070, 4991
OliI CACNNNNGTG 3 cut(s) 620, 3040, 5772
PaeI GCATGC 4 cut(s) 2753, 4203, 4666, 5528
PagI TCATGA 4 cut(s) 3495, 3862, 4342, 5026
PceI AGGCCT 1 cut(s) 3701
PciI ACATGT 3 cut(s) 333, 684, 5686
PciSI GCTCTTC 3 cut(s) 157, 3677, 5928
PctI GAATGC 4 cut(s) 1144, 2168, 3646, 5783
PdmI GAANNNNTTC 2 cut(s) 3104, 5275
PflMI CCANNNNNTGG 1 cut(s) 2460
PleI GAGTC 7 cut(s) 499, 1320, 2257, 2456, 2871, 4532, 5906
PpsI GAGTC 7 cut(s) 499, 1320, 2257, 2456, 2871, 4532, 5906
Ppu21I YACGTR 1 cut(s) 444
PpuMI RGGWCCY 1 cut(s) 6137
PscI ACATGT 3 cut(s) 333, 684, 5686
PshAI GACNNNNGTC 1 cut(s) 4833
PshBI ATTAAT 4 cut(s) 1838, 1911, 2306, 3525
PsiI TTATAA 1 cut(s) 1587
Psp124BI GAGCTC 1 cut(s) 3672
Psp1406I AACGTT 1 cut(s) 4041
Psp5II RGGWCCY 1 cut(s) 6137
Psp6I CCWGG 8 cut(s) 201, 809, 928, 1863, 5470, 5861, 6064, 6195
PspFI CCCAGC 3 cut(s) 462, 3818, 5091
PspGI CCWGG 8 cut(s) 201, 809, 928, 1863, 5470, 5861, 6064, 6195
PspOMI GGGCCC 1 cut(s) 1450
PspPPI RGGWCCY 1 cut(s) 6137
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PstI CTGCAG 3 cut(s) 4485, 4498, 5185
PstNI CAGNNNCTG 2 cut(s) 1826, 3758
PsuI RGATCY 5 cut(s) 366, 2847, 4074, 5467, 5542
PvuII CAGCTG 1 cut(s) 2720
RseI CAYNNNNRTG 8 cut(s) 620, 1002, 3040, 3226, 3574, 4463, 5486, 5772
SacI GAGCTC 1 cut(s) 3672
SapI GCTCTTC 3 cut(s) 157, 3677, 5928
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 7 cut(s) 500, 1321, 2258, 2457, 2871, 4532, 5907
ScrFI CCNGG 8 cut(s) 203, 811, 930, 1865, 5472, 5863, 6066, 6197
SexAI ACCWGGT 1 cut(s) 6064
SfcI CTRYAG 7 cut(s) 1680, 1849, 2559, 4481, 4494, 4964, 5181
SinI GGWCC 6 cut(s) 71, 188, 2345, 2710, 5963, 6137
SmiMI CAYNNNNRTG 8 cut(s) 620, 1002, 3040, 3226, 3574, 4463, 5486, 5772
SmlI CTYRAG 8 cut(s) 654, 797, 872, 1412, 3385, 3457, 3703, 3950
SmoI CTYRAG 8 cut(s) 654, 797, 872, 1412, 3385, 3457, 3703, 3950
SnaBI TACGTA 1 cut(s) 444
SpeI ACTAGT 1 cut(s) 1343
SphI GCATGC 4 cut(s) 2753, 4203, 4666, 5528
SseBI AGGCCT 1 cut(s) 3701
SspI AATATT 4 cut(s) 2807, 2934, 3061, 5099
SstI GAGCTC 1 cut(s) 3672
StuI AGGCCT 1 cut(s) 3701
StyD4I CCNGG 8 cut(s) 201, 809, 928, 1863, 5470, 5861, 6064, 6195
StyI CCWWGG 2 cut(s) 191, 718
TaiI ACGT 8 cut(s) 343, 446, 4044, 4511, 4851, 5502, 5793, 6115
TaqI TCGA 5 cut(s) 281, 2482, 3745, 4212, 5196
TaqII GACCGA 1 cut(s) 1495
TatI WGTACW 7 cut(s) 612, 1025, 1387, 1498, 2650, 3760, 5683
TauI GCSGC 2 cut(s) 94, 3624
TseFI GTSAC 5 cut(s) 336, 2260, 2822, 3583, 4504
TseI GCWGC 9 cut(s) 303, 830, 1130, 2519, 2717, 2870, 3150, 4688, 5736
Tsp45I GTSAC 5 cut(s) 336, 2260, 2822, 3583, 4504
TspGWI ACGGA 1 cut(s) 541
Van91I CCANNNNNTGG 1 cut(s) 2460
Vha464I CTTAAG 2 cut(s) 3385, 3703
VneI GTGCAC 2 cut(s) 564, 5227
VpaK11BI GGWCC 6 cut(s) 71, 188, 2345, 2710, 5963, 6137
VspI ATTAAT 4 cut(s) 1838, 1911, 2306, 3525
XbaI TCTAGA 1 cut(s) 1714
XcmI CCANNNNNNNNNTGG 1 cut(s) 3036
XmiI GTMKAC 2 cut(s) 1295, 5572
XmnI GAANNNNTTC 2 cut(s) 3104, 5275
ZrmI AGTACT 1 cut(s) 1027
Zsp2I ATGCAT 3 cut(s) 2751, 3070, 4991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.