RchiOBHm_Chr6g0245571

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000376
Physical Location & Seq
Reverse (-)
1356740 .. 1363561
6822 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22008

Sequence Viewer

Length: 3885 bp
ATGGCCCATTCTGGAGCTAACCTTAAACAGAGACAACCCAAGAAGAACAAGAAGAAGAGAAAGCAGAGCGGACCCGAAGAATCTGAAAGGCCACCCAAAACTAACCGCACCAGTTCATCAGAGAACGAGAATGAACCACAAGGCCAAAACTTTGAAGTTGGAAAAGCTGAAGAACCCAATCAAGGTGGTCCATGGAGGAACCTGGAGTTAATTCTGTCAATCCAGAACAGAGAGCTTGATCTTCACAAGAAGGTAGAACTAGCTCATGGTTTTGTGATGTCGAGGGTGAAGGAAGAAGGAGGCAGCAGTGATGAGGATAATCAAGCAGTGAACATGTCACGTATAATAATATTTGTCAATGATTGGGTCCAATCATTGTTGATTTCTGAAGGAAAGAAAGTTGAGAGTGGTGGAGAGAAGCATCAAGCTGAAGTTATATATGCGTATTTGGATTTAAGATGCTGGGAGATTTTCAAATTCTGTTTGGAGGAGTCTCTGAAATTAAATGTTTCGTTAAGTTTCTCACGGAACCTTTTACGCTCTATATGTTGGATTGCAAGGAGTGCACTGTCCTTATTGAACAAAACATCTATTGGTGAAGGGTTACAATTGTATGCTACTGTGCTTGATTGTATTTCTTTGGTATTTTCATCCCAAGAAGGCTTCTCAAATGAAAATTTAGACATGTGGATTACAACTGTAAGTTCAGTTCTTGACCTTGGACACAAATTTTACAGTGAAGCTCTTGTTAGTGGCAATGAGGGTGTTTTTAGGGCTCATGCTCGGAACGATGGATTTCGTGATTTTAGTAATAAGCTCCTTGAGCCTCTGTTGCATCTCTTGGGTCTCTTGCATCTCCAGATTGGTGGAAGTAATCCTGGTTGGACAAGAAAGTTACTGAAGCTTGTTGAAGATGTATTATCTCATGGGCTATATCACCCACTCCACATTGATGGGTTTTTGAGCTTGTGCAGTACTGAAAAAGATGCCACATCCAATGATGGGAAGTCAAAAGATTCCAAAACAATCAACCAGAGTTATCATAGACATCTGTTTGATAAAGTGGAAGGAATTTTAGCTGCAAAGAATGAATTTGCAGTAGAAAGCATAGTAGTACTGTTTCACTTGCTTACTGATCAAGTTAAAAAGCTAAAGAGGGATTCAGCGCTGACTGCAAGTACCAAAATGATGAGGAAGAGTGAAGGTTCAAGGCAGTTTGAAGATGGTTCATTGGGTCAAACGTCTATGATGTCTTCTGAGAGTCGTATTGTTGAAAAGAATTATTGCTCAACTAGTTTCAATCCAGGAACACGAAAGTCACTTCACGATTTTTTTGTACTGATTATGGAGCCTCTTTTGCTTGAGATCAATGGCTTTCTTGAAACTAAACTGAAAGTGGGTCCTGAATTGTTGGATATTCATTGCACACTTAAAGCTATAAATAATGTACTTTTGGTCTTTATGCATGAAAAGATTTATGTGAGAACAGAGGATGCCTCTGAGGGAGCTTCTCTTAAATTCTTGAAGAAGGTTTATAATATTATCATCTCATTGTCCTCAGATTTAATTCAGTTGTCAGTTACACACTCAGATAGTTTTACTCTAATAGCAAATGAGGTACTTTCTGCAATTGGCTATCTTTTGGAAATTAATTATGATGTTCTAGAGAATGATCTTGTAAGCTTGTGGTTGATGATCCTCTCCTACTGGGCCATTGGGCTTTCTTTGGTGGATTCTCCGGATAGATGCTCATTATTCCCCAAGATAACAGATATTGGATGCGAATTAATTATGCTCTATAGTCAACTTCGCCAGGATTGTTGTTGGCGGCTTATTAGATTATCAGAATGCTTCATACTCATCTTCAGATTGTTTGTGTCCTGTCAGAAGGCCTTAAGATGTATAATTACTGCTTGTGAGGAGACAGTAGCCAGTCGAACCTCAGACACTGTACTCTTTGAGGATTTATTTCCAGTTTCATGGCTTTACAAGTCAGTGCATATGGTTTCTGGGCTTCAAGAATCATTCTTCAAAGATATTTATCATCAAGTTCATGATATGATCTTGGAGTTAATGGATCACACATTTTATGTGTTTCTGACATTAAATAAATATCAATCCAATCATGCTGTTCGTTTCCTTGAGGTTGCTGAGCTGAATTTTGGATGTGCCCAAGAACAGAGAAGTTTATTGAATTTGTCCAATTATATTGAAGCCTGGAAAAGTGTAAACGTTGTTGCTAAGATTTTGAAGGAACAGATGCAGATCTTACTTGTAAATGTGAAGAATGGCATTTGTAATGCAGAAGAGGAAGTTTCTATTGATGCTTTAAATTTGAACAAGTTTGCTTCCATAATTTCGTGTTTTAGTTGGTTTTTATGGGGCCTAGCATGCGTTGTGATCAACACAGATGGGAGAAATAGTGATGAGAAAGCTAAGTTGTCAAGGTGGAAACTTGAACCAATCTCCCAACTCAACCTCTGTATAAAGTGTTCATTGAAGAAGACCGGCTTGAAAGATGCAAATAGTGTTGTTAGTGTTCTCACTGCTGTTGATTCATTTGAACTGCAATCTTTAAATAAGCCTTTGTTGAGAAACATGCTAAAAGGTGATTTCCCCAACGCAACATTTTTACTCAGGCAGTTGTTGATCGCCTCTTCTGCTATTTTGAGGCTAAATTTGCATATTAAGAGCGCTCACATGTTGTCAAGCTTGGTGCATAAGTTTGCTGGCATTATGCAAGTCTTGTTATTGGAATCAGCGGACGCAATTCAGGTGCCATACTTCTATTATTTTGTGTGTTTAGATGGTGTTCTGAAGTATCTGGAAGAATTAGGGAATCATTTTCCATTGACTAATCCTACCTTATCCAGAGATTTGTTGAGGTCATCATTCGCAGTGTTCATAAAGAAATCAACAGAGTTGCATCTCCAATCTGCAGTTCAAGCTATCGAAAGAGCCCTAGTTGGTGTGCGGGAAGGGTGCACAGTGAGGTATGACATATGTGCTGGAAGTGAAGATGGAGGAAGGGTTTCTTCAATTGTTGCATCTGGCATTGATTGCTTGGATTTAGTTCTGGAATTTGTTTCAGGACGCAATTTGATTGTGGTTAAAAAATACATTCAGAGGTTAATTGCCTGCATGCTCAATGTTATTCTGCATCTGCAGAGTCCACTAATCTTCTATGAGAGATTCACTCTGAGTAAAGATGATAGTGATCCAGATCCAGGAACTGTCATTCTTATGTGTGTTGACGTACTGGAAAGAATTTCTGGCAAGCATGCAATATACAAAATGGATCTCTGGCATGATTCTTCAACAAGTCCAAATAACCAACCATCCGACCCAGTAGCAAGTGTACATGTCTCTGGTATAGATAGGCAATACTCAATCGGCCTATATTCTGCTTGCTGCCGATTATTGCATAATGTTGTGAAGCATCACAAAAGTGAATGCGAGGGGTATGTTGCTCTACTTCAAGCTTCTGTTCGTGTTCTTCTTTATTGTTTGGAGACATTGGATGCTGTTGTGGTAGCCAGGGAAGGCTTCTTTTCATGGGAAGTAGAAGAAGGAGTCAAATGTGCATGTTCTCTCAGAAGGATTTATGAAGAGCTAAGACATCAAAAAGAGGTCTTTGGTCCACACTGTTACCACTTTTTAGCTTATTACATACGGGTTTATTCAGGATATGGCCCCCGCAAAACAGGCATCAAAAGGGAAATAGATGAAGCTCTGAGACCAGGTGTATATGCGCTGATAGATGTTTGCTCACCTGATGATCTTCTACGTCTTCATGCCACATTTGGAGAGGGTCCTTGTACAAACACTTTGGCAACCTTGAAGCACGATTACGAACTCAATTTCCAGTACCAGGGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

1294

Amino Acids

146.11

Weight (kDa)

6.31

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1123 - 1293 8.7e-33 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1536
AccB1I GGYRCC 1 cut(s) 2776
AccBSI CCGCTC 1 cut(s) 69
AccIII TCCGGA 1 cut(s) 1738
AciI CCGC 6 cut(s) 69, 106, 1828, 2762, 2974, 3697
AclI AACGTT 1 cut(s) 2232
AclWI GGATC 5 cut(s) 1690, 2085, 3212, 3218, 3306
AcuI CTGAAG 6 cut(s) 189, 408, 450, 920, 1849, 2837
AfeI AGCGCT 2 cut(s) 1167, 2695
AfiI CCNNNNNNNGG 4 cut(s) 182, 1002, 3227, 3871
AflII CTTAAG 1 cut(s) 1894
AflIII ACRYGT 4 cut(s) 333, 684, 2700, 3361
AhlI ACTAGT 1 cut(s) 1292
AjnI CCWGG 9 cut(s) 201, 877, 1303, 1812, 2216, 3226, 3536, 3739, 3870
AjuI GAANNNNNNNTTGG 2 cut(s) 2925, 2957
AleI CACNNNNGTG 1 cut(s) 3447
AloI GAACNNNNNNTCC 2 cut(s) 3846, 3878
Alw21I GWGCWC 2 cut(s) 568, 2987
Alw26I GTCTC 7 cut(s) 25, 498, 851, 1916, 3370, 3506, 3730
Alw44I GTGCAC 2 cut(s) 564, 2983
AlwI GGATC 5 cut(s) 1690, 2085, 3212, 3218, 3306
AlwNI CAGNNNCTG 2 cut(s) 1949, 3233
Aor13HI TCCGGA 1 cut(s) 1738
Aor51HI AGCGCT 2 cut(s) 1167, 2695
AoxI GGCC 8 cut(s) 3, 89, 142, 1710, 1890, 2383, 3394, 3691
ApaLI GTGCAC 2 cut(s) 564, 2983
ApeKI GCWGC 3 cut(s) 303, 1079, 3411
AseI ATTAAT 2 cut(s) 1650, 1787
Asp700I GAANNNNTTC 1 cut(s) 3031
AspLEI GCGC 3 cut(s) 1168, 2696, 3754
AsuHPI GGTGA 5 cut(s) 298, 608, 929, 2621, 3762
AvaII GGWCC 6 cut(s) 71, 188, 367, 1400, 3638, 3812
BaeGI GKGCMC 3 cut(s) 568, 2173, 2987
BanI GGYRCC 1 cut(s) 2776
BanII GRGCYC 2 cut(s) 778, 2962
BarI GAAGNNNNNNTAC 2 cut(s) 3833, 3865
BbsI GAAGAC 3 cut(s) 1245, 2510, 3782
Bbv12I GWGCWC 2 cut(s) 568, 2987
BbvI GCAGC 3 cut(s) 315, 1066, 3398
BccI CCATC 8 cut(s) 785, 947, 995, 1217, 2405, 2801, 3014, 3346
BciT130I CCWGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
BclI TGATCA 2 cut(s) 1135, 2400
BcoDI GTCTC 7 cut(s) 25, 498, 851, 1916, 3370, 3506, 3730
BcuI ACTAGT 1 cut(s) 1292
BfaI CTAG 5 cut(s) 260, 1293, 1664, 2387, 2963
BfmI CTRYAG 3 cut(s) 1798, 2937, 3164
BfoI RGCGCY 2 cut(s) 1169, 2697
BfrI CTTAAG 1 cut(s) 1894
BglII AGATCT 1 cut(s) 2265
BisI GCNGC 4 cut(s) 304, 1080, 1829, 3412
BlpI GCTNAGC 1 cut(s) 2151
BlsI GCNGC 4 cut(s) 305, 1081, 1830, 3413
BmcAI AGTACT 2 cut(s) 976, 1116
Bme1390I CCNGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
Bme18I GGWCC 6 cut(s) 71, 188, 367, 1400, 3638, 3812
BmrFI CCNGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
BmrI ACTGGG 2 cut(s) 1717, 3341
BmuI ACTGGG 2 cut(s) 1717, 3341
BpiI GAAGAC 3 cut(s) 1245, 2510, 3782
BplI GAGNNNNNCTC 4 cut(s) 1481, 1513, 3181, 3213
BpmI CTGGAG 3 cut(s) 33, 224, 842
Bpu1102I GCTNAGC 1 cut(s) 2151
BpuEI CTTGAG 3 cut(s) 842, 1382, 2162
BsaAI YACGTR 1 cut(s) 341
BsaBI GATNNNNATC 4 cut(s) 2040, 2264, 3216, 3222
BsaI GGTCTC 2 cut(s) 851, 3730
BsaJI CCNNGG 4 cut(s) 191, 718, 3537, 3871
BsaWI WCCGGW 1 cut(s) 1738
BsaXI ACNNNNNCTCC 4 cut(s) 2407, 2437, 3015, 3045
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 1002, 3227, 3871
Bse118I RCCGGY 1 cut(s) 2507
Bse1I ACTGG 7 cut(s) 111, 1712, 1932, 1973, 3264, 3347, 3865
Bse3DI GCAATG 2 cut(s) 763, 1420
Bse8I GATNNNNATC 4 cut(s) 2040, 2264, 3216, 3222
BseAI TCCGGA 1 cut(s) 1738
BseBI CCWGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
BseDI CCNNGG 4 cut(s) 191, 718, 3537, 3871
BseGI GGATG 7 cut(s) 650, 992, 1498, 1784, 2171, 3338, 3526
BseJI GATNNNNATC 4 cut(s) 2040, 2264, 3216, 3222
BseLI CCNNNNNNNGG 4 cut(s) 182, 1002, 3227, 3871
BseMI GCAATG 2 cut(s) 763, 1420
BseNI ACTGG 7 cut(s) 111, 1712, 1932, 1973, 3264, 3347, 3865
BseRI GAGGAG 2 cut(s) 503, 1934
BseSI GKGCMC 3 cut(s) 568, 2173, 2987
BseXI GCAGC 3 cut(s) 315, 1066, 3398
BseYI CCCAGC 1 cut(s) 462
BsgI GTGCAG 1 cut(s) 991
BshFI GGCC 8 cut(s) 5, 91, 144, 1712, 1892, 2385, 3396, 3693
BshNI GGYRCC 1 cut(s) 2776
BsiHKAI GWGCWC 2 cut(s) 568, 2987
BsiSI CCGG 2 cut(s) 1739, 2508
BslI CCNNNNNNNGG 4 cut(s) 182, 1002, 3227, 3871
BsmAI GTCTC 7 cut(s) 25, 498, 851, 1916, 3370, 3506, 3730
BsmI GAATGC 2 cut(s) 1853, 3458
BsnI GGCC 8 cut(s) 5, 91, 144, 1712, 1892, 2385, 3396, 3693
Bso31I GGTCTC 2 cut(s) 851, 3730
Bsp1286I GDGCHC 5 cut(s) 568, 778, 2173, 2962, 2987
Bsp13I TCCGGA 1 cut(s) 1738
Bsp1407I TGTACA 2 cut(s) 3358, 3818
Bsp1720I GCTNAGC 1 cut(s) 2151
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 6 cut(s) 69, 106, 1828, 2762, 2974, 3697
BspANI GGCC 8 cut(s) 5, 91, 144, 1712, 1892, 2385, 3396, 3693
BspEI TCCGGA 1 cut(s) 1738
BspHI TCATGA 1 cut(s) 2053
BspMAI CTGCAG 2 cut(s) 2941, 3168
BspPI GGATC 5 cut(s) 1690, 2085, 3212, 3218, 3306
BspQI GCTCTTC 1 cut(s) 3603
BspT107I GGYRCC 1 cut(s) 2776
BspTI CTTAAG 1 cut(s) 1894
BspTNI GGTCTC 2 cut(s) 851, 3730
BsrBI CCGCTC 1 cut(s) 69
BsrDI GCAATG 2 cut(s) 763, 1420
BsrFI RCCGGY 1 cut(s) 2507
BsrGI TGTACA 2 cut(s) 3358, 3818
BsrI ACTGG 7 cut(s) 111, 1712, 1932, 1973, 3264, 3347, 3865
BssAI RCCGGY 1 cut(s) 2507
BssECI CCNNGG 4 cut(s) 191, 718, 3537, 3871
BssT1I CCWWGG 2 cut(s) 191, 718
Bst2UI CCWGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
Bst6I CTCTTC 5 cut(s) 50, 1190, 2301, 2662, 3603
BstAFI CTTAAG 1 cut(s) 1894
BstAPI GCANNNNNTGC 2 cut(s) 563, 3060
BstAUI TGTACA 2 cut(s) 3358, 3818
BstBAI YACGTR 1 cut(s) 341
BstC8I GCNNGC 7 cut(s) 2392, 2731, 3139, 3143, 3278, 3282, 3409
BstDSI CCRYGG 1 cut(s) 191
BstF5I GGATG 7 cut(s) 650, 992, 1498, 1784, 2171, 3338, 3526
BstH2I RGCGCY 2 cut(s) 1169, 2697
BstHHI GCGC 3 cut(s) 1168, 2696, 3754
BstMAI GTCTC 7 cut(s) 25, 498, 851, 1916, 3370, 3506, 3730
BstNI CCWGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
BstNSI RCATGY 9 cut(s) 337, 688, 2394, 2602, 2704, 3145, 3284, 3365, 3588
BstSCI CCNGG 9 cut(s) 201, 877, 1303, 1812, 2216, 3226, 3536, 3739, 3870
BstSFI CTRYAG 3 cut(s) 1798, 2937, 3164
BstSLI GKGCMC 3 cut(s) 568, 2173, 2987
BstV1I GCAGC 3 cut(s) 315, 1066, 3398
BstV2I GAAGAC 3 cut(s) 1245, 2510, 3782
BstX2I RGATCY 3 cut(s) 2265, 3223, 3298
BstXI CCANNNNNNTGG 3 cut(s) 866, 953, 1980
BstYI RGATCY 3 cut(s) 2265, 3223, 3298
BsuRI GGCC 8 cut(s) 5, 91, 144, 1712, 1892, 2385, 3396, 3693
BtgI CCRYGG 1 cut(s) 191
BtsCI GGATG 7 cut(s) 650, 992, 1498, 1784, 2171, 3338, 3526
BtsI GCAGTG 4 cut(s) 313, 333, 2544, 2904
Cac8I GCNNGC 7 cut(s) 2392, 2731, 3139, 3143, 3278, 3282, 3409
CaiI CAGNNNCTG 2 cut(s) 1949, 3233
CciI TCATGA 1 cut(s) 2053
CfoI GCGC 3 cut(s) 1168, 2696, 3754
Cfr10I RCCGGY 1 cut(s) 2507
CseI GACGC 2 cut(s) 2774, 3102
CsiI ACCWGGT 1 cut(s) 3739
CspCI CAANNNNNGTGG 2 cut(s) 166, 201
DraI TTTAAA 2 cut(s) 2331, 2577
Eam1104I CTCTTC 5 cut(s) 50, 1190, 2301, 2662, 3603
EarI CTCTTC 5 cut(s) 50, 1190, 2301, 2662, 3603
Eco130I CCWWGG 2 cut(s) 191, 718
Eco147I AGGCCT 1 cut(s) 1892
Eco24I GRGCYC 2 cut(s) 778, 2962
Eco31I GGTCTC 2 cut(s) 851, 3730
Eco47I GGWCC 6 cut(s) 71, 188, 367, 1400, 3638, 3812
Eco47III AGCGCT 2 cut(s) 1167, 2695
Eco57I CTGAAG 6 cut(s) 189, 408, 450, 920, 1849, 2837
EcoO109I RGGNCCY 3 cut(s) 1400, 2383, 3812
EcoRII CCWGG 9 cut(s) 201, 877, 1303, 1812, 2216, 3226, 3536, 3739, 3870
EcoT14I CCWWGG 2 cut(s) 191, 718
EcoT22I ATGCAT 1 cut(s) 1467
EcoT38I GRGCYC 2 cut(s) 778, 2962
ErhI CCWWGG 2 cut(s) 191, 718
FalI AAGNNNNNCTT 4 cut(s) 2495, 2527, 3019, 3051
FauI CCCGC 2 cut(s) 2967, 3704
FauNDI CATATG 2 cut(s) 2001, 3002
FbaI TGATCA 2 cut(s) 1135, 2400
Fnu4HI GCNGC 4 cut(s) 304, 1080, 1829, 3412
FokI GGATG 7 cut(s) 637, 979, 1505, 1791, 2178, 3325, 3533
FriOI GRGCYC 2 cut(s) 778, 2962
Fsp4HI GCNGC 4 cut(s) 304, 1080, 1829, 3412
FspBI CTAG 5 cut(s) 260, 1293, 1664, 2387, 2963
GlaI GCGC 3 cut(s) 1167, 2695, 3753
GluI GCNGC 4 cut(s) 304, 1080, 1829, 3412
GsaI CCCAGC 1 cut(s) 466
GsuI CTGGAG 3 cut(s) 33, 224, 842
HaeII RGCGCY 2 cut(s) 1169, 2697
HaeIII GGCC 8 cut(s) 5, 91, 144, 1712, 1892, 2385, 3396, 3693
HapII CCGG 2 cut(s) 1739, 2508
HgaI GACGC 2 cut(s) 2774, 3102
HhaI GCGC 3 cut(s) 1168, 2696, 3754
Hin6I GCGC 3 cut(s) 1166, 2694, 3752
HinP1I GCGC 3 cut(s) 1166, 2694, 3752
HincII GTYRAC 2 cut(s) 1805, 3253
HindII GTYRAC 2 cut(s) 1805, 3253
HindIII AAGCTT 4 cut(s) 902, 1681, 2710, 3480
HpaII CCGG 2 cut(s) 1739, 2508
HphI GGTGA 5 cut(s) 298, 608, 929, 2621, 3762
Hpy166II GTNNAC 9 cut(s) 331, 566, 1805, 2230, 2985, 3173, 3253, 3359, 3641
Hpy8I GTNNAC 9 cut(s) 331, 566, 1805, 2230, 2985, 3173, 3253, 3359, 3641
HpyCH4IV ACGT 5 cut(s) 340, 1241, 2232, 3255, 3787
HpySE526I ACGT 5 cut(s) 340, 1241, 2232, 3255, 3787
HspAI GCGC 3 cut(s) 1166, 2694, 3752
Kpn2I TCCGGA 1 cut(s) 1738
Ksp22I TGATCA 2 cut(s) 1135, 2400
LguI GCTCTTC 1 cut(s) 3603
LmnI GCTCC 4 cut(s) 14, 822, 1348, 1505
Lsp1109I GCAGC 3 cut(s) 315, 1066, 3398
MabI ACCWGGT 1 cut(s) 3739
MaeI CTAG 5 cut(s) 260, 1293, 1664, 2387, 2963
MaeII ACGT 5 cut(s) 340, 1241, 2232, 3255, 3787
MaeIII GTNAC 6 cut(s) 336, 603, 894, 1317, 1579, 3647
MbiI CCGCTC 1 cut(s) 69
MfeI CAATTG 3 cut(s) 608, 1629, 3039
MflI RGATCY 3 cut(s) 2265, 3223, 3298
MhlI GDGCHC 5 cut(s) 568, 778, 2173, 2962, 2987
MlyI GAGTC 4 cut(s) 500, 1270, 3178, 3582
MmeI TCCRAC 5 cut(s) 139, 530, 863, 1392, 3366
Mph1103I ATGCAT 1 cut(s) 1467
MroI TCCGGA 1 cut(s) 1738
MroXI GAANNNNTTC 1 cut(s) 3031
MslI CAYNNNNRTG 3 cut(s) 951, 3242, 3447
MspA1I CMGCKG 1 cut(s) 2762
MspCI CTTAAG 1 cut(s) 1894
MspI CCGG 2 cut(s) 1739, 2508
MspR9I CCNGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
MunI CAATTG 3 cut(s) 608, 1629, 3039
Mva1269I GAATGC 2 cut(s) 1853, 3458
MvaI CCWGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
NcoI CCATGG 1 cut(s) 191
NdeI CATATG 2 cut(s) 2001, 3002
NmuCI GTSAC 2 cut(s) 336, 1317
NsiI ATGCAT 1 cut(s) 1467
NspI RCATGY 9 cut(s) 337, 688, 2394, 2602, 2704, 3145, 3284, 3365, 3588
OliI CACNNNNGTG 1 cut(s) 3447
PaeI GCATGC 3 cut(s) 2394, 3145, 3284
PagI TCATGA 1 cut(s) 2053
PceI AGGCCT 1 cut(s) 1892
PciI ACATGT 4 cut(s) 333, 684, 2700, 3361
PciSI GCTCTTC 1 cut(s) 3603
PctI GAATGC 2 cut(s) 1853, 3458
PdmI GAANNNNTTC 1 cut(s) 3031
PfoI TCCNGGA 2 cut(s) 1303, 3226
PkrI GCNGC 4 cut(s) 305, 1081, 1830, 3413
PleI GAGTC 4 cut(s) 499, 1269, 3177, 3581
PpsI GAGTC 4 cut(s) 499, 1269, 3177, 3581
Ppu21I YACGTR 1 cut(s) 341
PpuMI RGGWCCY 2 cut(s) 1400, 3812
PscI ACATGT 4 cut(s) 333, 684, 2700, 3361
PshBI ATTAAT 2 cut(s) 1650, 1787
PsiI TTATAA 1 cut(s) 1536
Psp1406I AACGTT 1 cut(s) 2232
Psp5II RGGWCCY 2 cut(s) 1400, 3812
Psp6I CCWGG 9 cut(s) 201, 877, 1303, 1812, 2216, 3226, 3536, 3739, 3870
PspFI CCCAGC 1 cut(s) 462
PspGI CCWGG 9 cut(s) 201, 877, 1303, 1812, 2216, 3226, 3536, 3739, 3870
PspPPI RGGWCCY 2 cut(s) 1400, 3812
PsrI GAACNNNNNNTAC 4 cut(s) 693, 725, 2476, 2508
PstI CTGCAG 2 cut(s) 2941, 3168
PstNI CAGNNNCTG 2 cut(s) 1949, 3233
PsuI RGATCY 3 cut(s) 2265, 3223, 3298
RseI CAYNNNNRTG 3 cut(s) 951, 3242, 3447
SapI GCTCTTC 1 cut(s) 3603
SatI GCNGC 4 cut(s) 304, 1080, 1829, 3412
ScaI AGTACT 2 cut(s) 976, 1116
SchI GAGTC 4 cut(s) 500, 1270, 3178, 3582
ScrFI CCNGG 9 cut(s) 203, 879, 1305, 1814, 2218, 3228, 3538, 3741, 3872
SduI GDGCHC 5 cut(s) 568, 778, 2173, 2962, 2987
SexAI ACCWGGT 1 cut(s) 3739
SfcI CTRYAG 3 cut(s) 1798, 2937, 3164
SinI GGWCC 6 cut(s) 71, 188, 367, 1400, 3638, 3812
SmiMI CAYNNNNRTG 3 cut(s) 951, 3242, 3447
SmlI CTYRAG 4 cut(s) 821, 1361, 1894, 2141
SmoI CTYRAG 4 cut(s) 821, 1361, 1894, 2141
SpeI ACTAGT 1 cut(s) 1292
SphI GCATGC 3 cut(s) 2394, 3145, 3284
SseBI AGGCCT 1 cut(s) 1892
SsiI CCGC 6 cut(s) 69, 106, 1828, 2762, 2974, 3697
SspI AATATT 2 cut(s) 351, 1540
SspMI CTAG 5 cut(s) 260, 1293, 1664, 2387, 2963
StuI AGGCCT 1 cut(s) 1892
StyD4I CCNGG 9 cut(s) 201, 877, 1303, 1812, 2216, 3226, 3536, 3739, 3870
StyI CCWWGG 2 cut(s) 191, 718
TaiI ACGT 5 cut(s) 343, 1244, 2235, 3258, 3790
TaqI TCGA 3 cut(s) 281, 1936, 2952
TatI WGTACW 7 cut(s) 974, 1114, 1336, 1447, 1951, 3358, 3818
TauI GCSGC 1 cut(s) 1831
TseFI GTSAC 2 cut(s) 336, 1317
TseI GCWGC 3 cut(s) 303, 1079, 3411
Tsp45I GTSAC 2 cut(s) 336, 1317
TspGWI ACGGA 1 cut(s) 541
Vha464I CTTAAG 1 cut(s) 1894
VneI GTGCAC 2 cut(s) 564, 2983
VpaK11BI GGWCC 6 cut(s) 71, 188, 367, 1400, 3638, 3812
VspI ATTAAT 2 cut(s) 1650, 1787
XbaI TCTAGA 1 cut(s) 1663
XceI RCATGY 9 cut(s) 337, 688, 2394, 2602, 2704, 3145, 3284, 3365, 3588
XmnI GAANNNNTTC 1 cut(s) 3031
XspI CTAG 5 cut(s) 260, 1293, 1664, 2387, 2963
ZrmI AGTACT 2 cut(s) 976, 1116
Zsp2I ATGCAT 1 cut(s) 1467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.