Rroxscaffold_3G00242140

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000376
Physical Location & Seq
Forward (+)
34535731 .. 34549236
13506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242140.1

Sequence Viewer

Length: 6408 bp
ATGGCCGATTCTGGAGCTAACCCTAAACAGAGACAACCCAAGAAGAACAATAAGAAGAGAAAGCAGAGCGGACCCCAAGAATCTGAAAGGCCACCCAAGACTAACCGCACCAATTCATCAGAGAACGAGAATGAACCACAAGACCAAAACTTTGAAGTTGGAAAAGCTGAAAAACCCAATCAAGGTGGTCCATGGAGGAACCTGGAGTTAATTCTGTCAATCCAGAACAGAGAGCTTGATCTTCACAAGAAGGTGGAACTAGCTCATGGTTTTGTGATGTCGAGGGTGAAGGAAGAAGGAGGCAGCAGTGATGAGGATAATCAAGCAGTGAACATGTCACGTTTAGTAATTTTTGTCAGTGATTGGATCCAGTCATTGTTGATTTCCGAAGGAAATAAAGTTGAGAGCGGTGGAGAGAAGCATCAAGCTGAAGTTATTTATACGTATTTGGATTTAAGATGCTGGGAGATTTTCAAATTCTGTTTGGAGGAGTCTCTGAAATTAAATGTTTTGTTAAGTTTCTCACGGAACCTTTTACGCTCTATATGTTGGATTGCAAGGAGCGCACTGTCCTTATTGAACAAAAAAATTATTGGTGAAGGGTTACAATTGTACACTACTGTGCTTGATTGTATTTCTTTGGTATTTTCATCCCAAGAAGGCTTCTCAAATGAAAATTTAGACATGTGGATTATAACTGTAAGTTCAGTTCTTGACCTTGGACACAAATTTTACAGTGAAGCTCCTGTTGGTGGCAATGAGGGTGTATTTGTTTTCCGATTCTTGTGCTTGGTGCTTGAGCCATTTGCCAGGTTTTTAAGGGCTCACGCAGCTCGGAAAGATGGATTTCGTGATTTTAGTAATAAGCTCCTTGAGCCTCTGTTGCACCTCTTGGGTCTCTTGCATCTCCAGATTGGTGGAAGTAATCCTGGTTGGAGAAGAAACTTACTGAAGCTTGTTGAAGATGCATTATCTCATGGGCTTTATCACCCGCTCCACATTGATGGGTTTTTGAGCTTATGCAGTACTGAAAAAGACACCACATCCAATGAGAGGACTGTGTCGGTTGTAAGAGATGTGAATCCGCCTGTCCCCGAATCCGGCTGCTGGTGCGGGCTTATACCTGGCTGGATGGGACTTTCTAAATGCGGGGCGAAATCCGGAAGCCAATTGCTGGCTCTGAATAACCAGCCGTTCAATTCAATTCTTCGGAAGTCAAAAGATTCCAAAACAATCAACCAGAGTTATCATAGACATTTATTTGATAAAGTGGAAGGAATTTTAGCTGCAAAGAATGCATTTGCAGTAGAAAGCATAGGAGTATTGTTTCACTTGCTTATTGATCAAGTTAAAAAGCTAAAGAGGGATTCAGCGCTGACTGCCAGTACCAAAATGATGGGGAAGAGTGAAGGTTCAAGGCAGTTAGAAGATTGTTCATTGGGTCAAACGTCTATGATGTCTTCTGAGAGTCGTATTGTTGAAAAGAATTATTGCTCAACTAGTTTCAATCCAGGAACACGAAAGTCACTTCACGATTTTTTTGTACTGATTATGGAGCCTCTTTTGCTTGAGATCAATGGCTTTCTTGAAACTAAACTGAAAGTGGGCCCTGAATTGTTGGATATTCATTGCACACTTAAAGCTATAAATAATGTACTTTCGGTCTTTATGCATGAAAAGATTTACGTGAGAACAGAGGATGCCTCTGAGGGAGCTTCTCTTAATTTCTTGAAGAAGGTTTATAATATTATCATCTCATTGTCCTCAGATTTAATTCAGTTGTCAGTTACACACTTAGATAGTTTTACTCTAATAGCAAGTGAGGTACTTTCTGCTGTTGGCTATCTTTTGGAAATTGAGTATGATGTTCTGGAGAATGATCTAGTAAGCTTGTGGTTAATGATCCTCTCTTACTGGGCCATTGGGCTTTCTTTGGTGGATTCTCCGGATAGATGCACATTATTCTCCAAGATAACAGCTATTGGATGCCAATTAATTATGCTCTATAGTCAACTTCGCCAGGTGAATAATACTATCTTTGCATTGTGTAAAGCAATAAGGGTTATTAATTTACATAATAGTGATGGTGAACTGAACTATACTAGATTTGTCATTCCTTTTCATGGTGAAGAATATGCAAAAGCTGTGGAAATGCTATTATCTGCACATGAATTCAAAATTGCAATCCATAAAGCAATGAAGTCTATACCAGAAGGACAAGCAAGTCGGTGTATTCGGCAGTTAACATTGGATATATCAGAATCTGTAGAGTGGATGAAAGTTAGTTGTTCAGAGGCTGATGAAAAAGAATTCTCTGAATGCCATCTAAGTAGCTTGCACAGTTTTAATCTGGAAGCTGAACTTTTCGGGAGAGGGTTGTCAGAAATATATGCATTATTCCTAGAGTCATTGATTGTCACAACCGGAAACTGTAACCTTCTTGGCGCTTCTATAAAGGAATTAATTAGAGTGATTTTCCCTTGCATGAGCAATCTGGTTGGACCACAGCAGGAGGATGCTGTAAACAAATTCCTATGGTCTGTCGCGGTAAAAGATTCTGACAATGTGATCGCTAGAAACAAAAACAAATATCTGATATTTGGAGTCTCCACTTATTGGGTAGTTTTGTTCTTCTTTCGATTGTATACCTCCAGCCGATGCTTATATAGGCAAGCAGCAATCCTTATGCCTCCAGATTTATCAAGAAAGATGTCTGCAGAAATGGGAGATTCGTTCTCCTCATCCTCTGGCAGGGATTGGATGGAAATGTCTGATTGGATCAATGGGGGATTTTTCTCTTGGATTGTACAACCTTCAGCTTCTCTTCTTTCTGTTATTCAATCTGTTTCAAACATCTTTTGTAAGGACCGTGCAGCTGATTCTTGTCCTTTAACTTATGTAATGCATGCTATTGCTTGTGAAAGGCTTGTGGATTTGAATAGTCGTATAAAGTCTTTTGAATATTTGATTGAAAATGGTGACAATCTGGTGCAAATTGCGGAGATCTCTTCTTTGAGGCAAGAGGCAGCAGGACTCACGGGATTCATGATGGAGCACCTCTCATTAGTGTCTGAAGATCAACAGCGAATATTCATTTCTGCTGATACGACTAATAATAAGATGGTTAACTATGAAAGTGATGAATGGGATTTCAGTATTTGCTCTGTGAACAAGAAGTCATTGCCAACTGCACTTTGGTGGGTTGTTTGCCAAAATATTCATGCATGGTGCCCTCATGCCTCTAAAAAGGATTTGAAGAGATTCCTTTCTTTTTTAATCCATACTTCAATTCCCTATGAAAGAAGCAGCTTTGGGGTGGTTATAGAGCACAAGAACCATGAAGCTGACAGGCTGAAGATTGTGGCATTGCATCAAATCTCATCACAGTGCTTTATTGATTCCAGTCTGTATGAGCAAAGAGTAAGCATCTTTCAAAGATCAAATGGTGCTTCCCCTGACCCTCACGTGATGTATTTTGCAAAGAGGTTCTGCCGTGCATTGGAGAAATCTGCATTACCATTCATCAGCGACTTTCCATCCGGCAATGTTAAATTCAAATCATCACCTAATTGGCCGAATGTCTTAAGTGATTTAGAGAACTCATCATTGGCTATTTCCTGCAACAAACTCAATGTATTTGATTGTTCCTCAGCCTCAAGTTGCACGGGACAAAACTCCCAACCATCTACCATCATGAAATTTACTGCTTGTCAGAGTTTGCTTAATCTTTTGAGTTGTATGCCAAAAGGTCATTTGGACACGAGATCATTTTCACGTTATGTGACTTCTATTCTGAACCTCGAAAGGATTGTTGTTGGCGGCTTATTAGATTATCAGAATGCTTCATACTCAACTTATTATTATGAGCTCTTCAGATTGTTTGTGTCCTGTCGGAAGGCCTTAAGATGTATAATTACTGCTTGTGAGGAGACAGTAGCCAGTCGAACCTCAGACACCGTACTCTTTGAGGATTTATTTCCAGTTTCATGGCTTTACAAGTCAGTGCATATGGTTGCTGGGCTTCAAGAATCATTCTCAAAAGATATTTATCATCAAGTCCATGATATGATCTTGGCGTTAATGGATCACACATTTTATGTGTTTCTGACATTAAATAAATATCAATCCAATCATGCTGTTCGTTTCCTTGAGGTTGCTGAGCTGAATTTTGGATGTGCCCAAGAACAGAGAAGTTTATTGAATTCGTCCAATTATTTTGAAGCCTGGAAAAGTGTAAACATTGTTGCTAAGATGTTGAAGGAACAGATGCAGATCTTACTTGTAAATGTGAAGAATGGCATTTGTAATGGAAAACAGGAAGTTTCTGTTGATGCTTTAAATTTGAACAAGTTTGCTTCCATAATTTCGTGTTTTAGTGGGTTTTTATGGGGCCTAGCTTGCGTTGTGATCGACACAGATGGGAGAAATAGTGATGAGAAAGCTAAGTTGTCGAGGTGGAAACTTGAACCAATCTCCGAACTCAACCTCTGTATAAAGGTTTTTGAAGAAATTTCTAGTCTTCTACTACAAATGTTTATTCTTGACGATAATCAGCAGTCCACAGCTATATGTCATGCCTATAATCTTCAGAAGTCAGGCTACAATGTAGATTTGTTGGGTGCAGAGAAGATTTTTCCGGATGGCAATGATACTGACACTGATATGGCATGTGGTGGACTGCAGGATGAATCTGCAGTTGCAGTGACGTGCTCAGCATCATCTGACATCTGTGATGACTCTGTAATTGGTAGTGTTCATAGAAGAAGACCGCGCTTGAAAGATGCAAATAGTGTTGTTAGTGTTCTCACTGCTGTTGATTCATTTGAACTGCAATTTTTAAATAAGCCTTTGTTTAGAAGCATGCTAAAAGGTGATTTCCCCAACGCAGCATTTTTACTCAGGCAGTTGTTAATCGCCTCTTCTGCTATTTTGAGGCTAAATTTGCATATTAAGAGCGCTCCCATGTTGTCAAGCTTGGTGCATAAGTTTGCTGGCATTATGCAAGTCTTGTTATTGGAATCAGCGGACCCAATTCAGGTGCCACACGTCTACTATTTTGTGTGTTTAGATGGTGTTCTGAAGTATCTGGAAGAATTAGGGAATCATTTTCCTTTGTCTAATCCTGCCTCATCCAGAGATTTGTTTGACAAGATGGTTCAGCTACAGTTACGGGCCCTAGGGAAATGCATAACTTTACAAGGGAAAAGAGCAACTCTTGCATCTCATGAAACAAGCACCAACACACCCATAGGAGAAAGGAGATTTTCTGAAGCATCATCTTTTTCTGGCTGGGAATATTTATTGGATGAATTCAAAGCTAGGTTGAGGTCATCATTCGCCGTGTTCATAAAGAAATCAACAGAGTTGCATCTCCAATCTGCAGTTCAAGCTATCGAAAGAGCCCTAGTTGGTGTGCGGGAAGGGTGCACAGTGAGGTATGACATATGTGCTGGAAATGAAGATGGAGGAAAGGTTTCTTCAATTGTTGCATCTGGCATTGATTGCTTGGATTTGGTTCTGGAATTTGTTTCAGGACGCAATTTGATTGTGGTTAAAAAATACATTCAGAGGTTAATTGCCTGCATGTTCAATGTTATTCTGCATCTGCAGAGTCCACTAATCTTCTACGAGAGATTCACTCAGAGTAAAGATGATAGTAATCCGGATCCAGGAACAGTCATTCTTATGTGTGTTGACGTACTGGCAAGAATTTCTGGCAAGCATGCAATATACAAAATGGATCTCTGGCATGTAGCACACTCGTTACGTATACCTTCAGCACTTTTTCAAGATTTCCATCTGTTAAAAGATTCTAAACGTCCTGTTCCAAATGATTCTTCAGCAAGTCTAAATAACCAACCATCCGACCCAGTAGCAAGTGTGCATGTCTCTGGTATAGATAGGCAATACTCAATCGGCCTATATTGTGCTTGCTGCCGATTATTGCATAATGTTGTGAAGCATCACAAAAGTGAATGCGAGGGGTATGTTGCTCTACTTCAAGCTTCTGTTCGTGTTCTTCTTTATTGTTTGGAGACATTGGATGCTGTTGTGGTAGCCAGGGAAGGCTTCTTTTCATGGGAAGTAGAAGAAGGAGTCAAATGTGCATGTTTTCTCAGAAGGATTTATGAAGAGCTAAGACATCAAAAAGAAGTCTTTGGTCCACACTGTTACCACTTTTTAGCTTATTACATACGGGTTTATTCCGGATATGGCCCCCGTAAAACAGGCATCAAAAGGGAAATAGATGAAGCTCTAAGACCAGGTGTATATGCGCTGATAGATGTTTGCTCACCTGATGATCTTCAACGTCTTCATACCACATTTGGAGAGGGTCCTTGCAGAAACACTTTGGCAACCTTGAAGCACGATTACGAACTCAATTTCCAGTACCAGGGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

2135

Amino Acids

240.14

Weight (kDa)

6.43

Isoelectric Point (pI)

46.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1896 - 2134 8e-37 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 695, 1743
AasI GACNNNNNNGTC 1 cut(s) 2223
AccB1I GGYRCC 2 cut(s) 3228, 5038
AccB7I CCANNNNNTGG 2 cut(s) 1174, 2616
AccBSI CCGCTC 3 cut(s) 69, 408, 994
AccI GTMKAC 3 cut(s) 2645, 5049, 5770
AccII CGCG 2 cut(s) 2546, 4771
AccIII TCCGGA 5 cut(s) 1160, 1945, 4636, 5662, 6206
AclWI GGATC 8 cut(s) 361, 374, 1897, 2786, 4092, 5660, 5673, 5748
AcoI YGGCCR 2 cut(s) 3, 3572
AcvI CACGTG 1 cut(s) 3466
AfeI AGCGCT 2 cut(s) 1374, 4957
AflII CTTAAG 2 cut(s) 3583, 3901
AflIII ACRYGT 3 cut(s) 333, 684, 5044
AhlI ACTAGT 1 cut(s) 1499
AjiI CACGTC 2 cut(s) 4707, 5047
AjuI GAANNNNNNNTTGG 8 cut(s) 732, 764, 3590, 3622, 5367, 5399, 5821, 5853
AleI CACNNNNGTG 3 cut(s) 620, 3196, 5970
AloI GAACNNNNNNTCC 2 cut(s) 6369, 6401
Alw21I GWGCWC 5 cut(s) 3057, 3330, 3870, 4712, 5429
Alw26I GTCTC 7 cut(s) 25, 498, 902, 2611, 3923, 5893, 6029
Alw44I GTGCAC 1 cut(s) 5425
AlwI GGATC 8 cut(s) 361, 374, 1897, 2786, 4092, 5660, 5673, 5748
AlwNI CAGNNNCTG 2 cut(s) 2264, 2297
Aor13HI TCCGGA 5 cut(s) 1160, 1945, 4636, 5662, 6206
Aor51HI AGCGCT 2 cut(s) 1374, 4957
ApaI GGGCCC 2 cut(s) 1610, 5176
ApaLI GTGCAC 1 cut(s) 5425
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AseI ATTAAT 3 cut(s) 1994, 2067, 2462
Asp700I GAANNNNTTC 2 cut(s) 3260, 5473
AspA2I CCTAGG 1 cut(s) 5176
AspLEI GCGC 6 cut(s) 566, 1375, 2447, 4773, 4958, 6277
AvaII GGWCC 7 cut(s) 71, 188, 2501, 2866, 5026, 6161, 6335
AvrII CCTAGG 1 cut(s) 5176
BaeGI GKGCMC 5 cut(s) 1610, 3233, 4180, 5176, 5429
BamHI GGATCC 2 cut(s) 366, 5665
BanI GGYRCC 2 cut(s) 3228, 5038
BanII GRGCYC 5 cut(s) 826, 1610, 3870, 5176, 5404
BarI GAAGNNNNNNTAC 4 cut(s) 4583, 4615, 6356, 6388
BauI CACGAG 1 cut(s) 3760
BbrPI CACGTG 1 cut(s) 3466
BbsI GAAGAC 4 cut(s) 1452, 4511, 4771, 6305
Bbv12I GWGCWC 5 cut(s) 3057, 3330, 3870, 4712, 5429
BbvCI CCTCAGC 1 cut(s) 3649
BceAI ACGGC 3 cut(s) 1177, 3477, 5324
BcgI CGANNNNNNTGC 2 cut(s) 768, 802
BclI TGATCA 1 cut(s) 1342
BcoDI GTCTC 7 cut(s) 25, 498, 902, 2611, 3923, 5893, 6029
BcuI ACTAGT 1 cut(s) 1499
BfmI CTRYAG 8 cut(s) 2005, 2265, 2715, 4679, 4692, 5162, 5379, 5606
BfoI RGCGCY 3 cut(s) 1376, 2448, 4959
BfrI CTTAAG 2 cut(s) 3583, 3901
BglII AGATCT 2 cut(s) 3003, 4272
BlnI CCTAGG 1 cut(s) 5176
BlpI GCTNAGC 2 cut(s) 4158, 4711
BmcAI AGTACT 1 cut(s) 1027
Bme18I GGWCC 7 cut(s) 71, 188, 2501, 2866, 5026, 6161, 6335
BmgBI CACGTC 2 cut(s) 4707, 5047
BmrI ACTGGG 2 cut(s) 1924, 5864
BmuI ACTGGG 2 cut(s) 1924, 5864
BpiI GAAGAC 4 cut(s) 1452, 4511, 4771, 6305
BplI GAGNNNNNCTC 6 cut(s) 1688, 1720, 3044, 3076, 5623, 5655
BpmI CTGGAG 6 cut(s) 33, 224, 893, 1892, 2635, 2676
Bpu10I CCTNAGC 1 cut(s) 3649
Bpu1102I GCTNAGC 2 cut(s) 4158, 4711
BpuEI CTTGAG 5 cut(s) 818, 893, 1589, 3640, 4169
BsaAI YACGTR 4 cut(s) 444, 1687, 3466, 5768
BsaBI GATNNNNATC 5 cut(s) 1080, 4047, 4271, 5658, 5796
BsaI GGTCTC 1 cut(s) 902
BsaJI CCNNGG 5 cut(s) 191, 718, 5176, 6060, 6394
BsaWI WCCGGW 6 cut(s) 1160, 1945, 2423, 4636, 5662, 6206
BsaXI ACNNNNNCTCC 4 cut(s) 4414, 4444, 5457, 5487
Bse1I ACTGG 9 cut(s) 370, 1383, 1919, 3402, 3939, 3980, 5706, 5870, 6388
Bse3DI GCAATG 7 cut(s) 763, 1627, 2202, 3179, 3365, 3550, 4651
Bse8I GATNNNNATC 5 cut(s) 1080, 4047, 4271, 5658, 5796
BseAI TCCGGA 5 cut(s) 1160, 1945, 4636, 5662, 6206
BseDI CCNNGG 5 cut(s) 191, 718, 5176, 6060, 6394
BseJI GATNNNNATC 5 cut(s) 1080, 4047, 4271, 5658, 5796
BseMI GCAATG 7 cut(s) 763, 1627, 2202, 3179, 3365, 3550, 4651
BseNI ACTGG 9 cut(s) 370, 1383, 1919, 3402, 3939, 3980, 5706, 5870, 6388
BseRI GAGGAG 3 cut(s) 503, 2728, 3941
BseSI GKGCMC 5 cut(s) 1610, 3233, 4180, 5176, 5429
BseYI CCCAGC 3 cut(s) 462, 4016, 5289
BsgI GTGCAG 4 cut(s) 2148, 2892, 3174, 4641
Bsh1236I CGCG 2 cut(s) 2546, 4771
BshNI GGYRCC 2 cut(s) 3228, 5038
BsiHKAI GWGCWC 5 cut(s) 3057, 3330, 3870, 4712, 5429
BsiSI CCGG 8 cut(s) 1101, 1161, 1946, 2424, 3540, 4637, 5663, 6207
BslFI GGGAC 3 cut(s) 1076, 1149, 3681
BsmAI GTCTC 7 cut(s) 25, 498, 902, 2611, 3923, 5893, 6029
BsmFI GGGAC 3 cut(s) 1076, 1149, 3681
BsmI GAATGC 4 cut(s) 1300, 2324, 3844, 5981
Bso31I GGTCTC 1 cut(s) 902
Bsp120I GGGCCC 2 cut(s) 1606, 5172
Bsp13I TCCGGA 5 cut(s) 1160, 1945, 4636, 5662, 6206
Bsp1407I TGTACA 2 cut(s) 612, 2806
Bsp1720I GCTNAGC 2 cut(s) 4158, 4711
Bsp19I CCATGG 1 cut(s) 191
BspEI TCCGGA 5 cut(s) 1160, 1945, 4636, 5662, 6206
BspFNI CGCG 2 cut(s) 2546, 4771
BspHI TCATGA 3 cut(s) 3045, 3693, 5224
BspMAI CTGCAG 5 cut(s) 2719, 4683, 4696, 5383, 5610
BspPI GGATC 8 cut(s) 361, 374, 1897, 2786, 4092, 5660, 5673, 5748
BspQI GCTCTTC 2 cut(s) 3875, 6126
BspT107I GGYRCC 2 cut(s) 3228, 5038
BspTI CTTAAG 2 cut(s) 3583, 3901
BspTNI GGTCTC 1 cut(s) 902
BsrBI CCGCTC 3 cut(s) 69, 408, 994
BsrDI GCAATG 7 cut(s) 763, 1627, 2202, 3179, 3365, 3550, 4651
BsrGI TGTACA 2 cut(s) 612, 2806
BsrI ACTGG 9 cut(s) 370, 1383, 1919, 3402, 3939, 3980, 5706, 5870, 6388
BssECI CCNNGG 5 cut(s) 191, 718, 5176, 6060, 6394
BssNAI GTATAC 2 cut(s) 2646, 5771
BssSI CACGAG 1 cut(s) 3760
BssT1I CCWWGG 3 cut(s) 191, 718, 5176
Bst1107I GTATAC 2 cut(s) 2646, 5771
Bst2BI CACGAG 1 cut(s) 3760
Bst6I CTCTTC 8 cut(s) 50, 1397, 2829, 3013, 3251, 3875, 4924, 6126
BstAFI CTTAAG 2 cut(s) 3583, 3901
BstAPI GCANNNNNTGC 3 cut(s) 1295, 5216, 5502
BstAUI TGTACA 2 cut(s) 612, 2806
BstBAI YACGTR 4 cut(s) 444, 1687, 3466, 5768
BstDSI CCRYGG 1 cut(s) 191
BstENI CCTNNNNNAGG 1 cut(s) 2749
BstFNI CGCG 2 cut(s) 2546, 4771
BstH2I RGCGCY 3 cut(s) 1376, 2448, 4959
BstHHI GCGC 6 cut(s) 566, 1375, 2447, 4773, 4958, 6277
BstMAI GTCTC 7 cut(s) 25, 498, 902, 2611, 3923, 5893, 6029
BstSFI CTRYAG 8 cut(s) 2005, 2265, 2715, 4679, 4692, 5162, 5379, 5606
BstSLI GKGCMC 5 cut(s) 1610, 3233, 4180, 5176, 5429
BstSNI TACGTA 2 cut(s) 444, 5768
BstUI CGCG 2 cut(s) 2546, 4771
BstV2I GAAGAC 4 cut(s) 1452, 4511, 4771, 6305
BstX2I RGATCY 5 cut(s) 366, 3003, 4272, 5665, 5740
BstXI CCANNNNNNTGG 4 cut(s) 917, 1004, 1396, 3987
BstYI RGATCY 5 cut(s) 366, 3003, 4272, 5665, 5740
BstZ17I GTATAC 2 cut(s) 2646, 5771
BtgI CCRYGG 1 cut(s) 191
BtrI CACGTC 2 cut(s) 4707, 5047
BtsI GCAGTG 4 cut(s) 313, 333, 4707, 4806
CaiI CAGNNNCTG 2 cut(s) 2264, 2297
CciI TCATGA 3 cut(s) 3045, 3693, 5224
CfoI GCGC 6 cut(s) 566, 1375, 2447, 4773, 4958, 6277
CseI GACGC 1 cut(s) 5544
CsiI ACCWGGT 1 cut(s) 6262
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 2127, 2162
DraI TTTAAA 2 cut(s) 4338, 4839
DrdI GACNNNNNNGTC 1 cut(s) 2223
DseDI GACNNNNNNGTC 1 cut(s) 2223
EaeI YGGCCR 2 cut(s) 3, 3572
Eam1104I CTCTTC 8 cut(s) 50, 1397, 2829, 3013, 3251, 3875, 4924, 6126
EarI CTCTTC 8 cut(s) 50, 1397, 2829, 3013, 3251, 3875, 4924, 6126
EciI GGCGGA 1 cut(s) 1074
Ecl136II GAGCTC 1 cut(s) 3868
Eco105I TACGTA 2 cut(s) 444, 5768
Eco130I CCWWGG 3 cut(s) 191, 718, 5176
Eco147I AGGCCT 1 cut(s) 3899
Eco24I GRGCYC 5 cut(s) 826, 1610, 3870, 5176, 5404
Eco31I GGTCTC 1 cut(s) 902
Eco47I GGWCC 7 cut(s) 71, 188, 2501, 2866, 5026, 6161, 6335
Eco47III AGCGCT 2 cut(s) 1374, 4957
Eco53kI GAGCTC 1 cut(s) 3868
Eco72I CACGTG 1 cut(s) 3466
EcoICRI GAGCTC 1 cut(s) 3868
EcoNI CCTNNNNNAGG 1 cut(s) 2749
EcoO109I RGGNCCY 4 cut(s) 1607, 4390, 5173, 6335
EcoRI GAATTC 4 cut(s) 2171, 2309, 4201, 5309
EcoT14I CCWWGG 3 cut(s) 191, 718, 5176
EcoT22I ATGCAT 7 cut(s) 970, 1300, 1674, 2395, 2907, 3226, 5189
EcoT38I GRGCYC 5 cut(s) 826, 1610, 3870, 5176, 5404
ErhI CCWWGG 3 cut(s) 191, 718, 5176
FalI AAGNNNNNCTT 2 cut(s) 2346, 2378
FaqI GGGAC 3 cut(s) 1076, 1149, 3681
FauI CCCGC 4 cut(s) 999, 1106, 1142, 5409
FauNDI CATATG 2 cut(s) 4008, 5444
FbaI TGATCA 1 cut(s) 1342
FblI GTMKAC 3 cut(s) 2645, 5049, 5770
FriOI GRGCYC 5 cut(s) 826, 1610, 3870, 5176, 5404
GlaI GCGC 6 cut(s) 565, 1374, 2446, 4772, 4957, 6276
GsaI CCCAGC 3 cut(s) 466, 4020, 5293
GsuI CTGGAG 6 cut(s) 33, 224, 893, 1892, 2635, 2676
HaeII RGCGCY 3 cut(s) 1376, 2448, 4959
HapII CCGG 8 cut(s) 1101, 1161, 1946, 2424, 3540, 4637, 5663, 6207
HgaI GACGC 1 cut(s) 5544
HhaI GCGC 6 cut(s) 566, 1375, 2447, 4773, 4958, 6277
Hin6I GCGC 6 cut(s) 564, 1373, 2445, 4771, 4956, 6275
HinP1I GCGC 6 cut(s) 564, 1373, 2445, 4771, 4956, 6275
HincII GTYRAC 4 cut(s) 2012, 2244, 3127, 5695
HindII GTYRAC 4 cut(s) 2012, 2244, 3127, 5695
HindIII AAGCTT 4 cut(s) 953, 1888, 4972, 6003
HpaI GTTAAC 2 cut(s) 2244, 3127
HpaII CCGG 8 cut(s) 1101, 1161, 1946, 2424, 3540, 4637, 5663, 6207
HspAI GCGC 6 cut(s) 564, 1373, 2445, 4771, 4956, 6275
Kpn2I TCCGGA 5 cut(s) 1160, 1945, 4636, 5662, 6206
Ksp22I TGATCA 1 cut(s) 1342
KspAI GTTAAC 2 cut(s) 2244, 3127
LguI GCTCTTC 2 cut(s) 3875, 6126
LmnI GCTCC 9 cut(s) 14, 561, 748, 873, 999, 1555, 1712, 3052, 4963
MabI ACCWGGT 1 cut(s) 6262
MbiI CCGCTC 3 cut(s) 69, 408, 994
MfeI CAATTG 3 cut(s) 608, 1169, 5481
MflI RGATCY 5 cut(s) 366, 3003, 4272, 5665, 5740
MlyI GAGTC 8 cut(s) 500, 1477, 2414, 2613, 3027, 4730, 5620, 6105
MmeI TCCRAC 7 cut(s) 139, 530, 914, 1599, 2479, 3872, 5889
Mph1103I ATGCAT 7 cut(s) 970, 1300, 1674, 2395, 2907, 3226, 5189
MroI TCCGGA 5 cut(s) 1160, 1945, 4636, 5662, 6206
MroXI GAANNNNTTC 2 cut(s) 3260, 5473
MslI CAYNNNNRTG 8 cut(s) 620, 1002, 2079, 3196, 3385, 4661, 5684, 5970
MspA1I CMGCKG 2 cut(s) 2876, 5024
MspCI CTTAAG 2 cut(s) 3583, 3901
MspI CCGG 8 cut(s) 1101, 1161, 1946, 2424, 3540, 4637, 5663, 6207
MunI CAATTG 3 cut(s) 608, 1169, 5481
Mva1269I GAATGC 4 cut(s) 1300, 2324, 3844, 5981
MvnI CGCG 2 cut(s) 2546, 4771
NcoI CCATGG 1 cut(s) 191
NdeI CATATG 2 cut(s) 4008, 5444
NmuCI GTSAC 6 cut(s) 336, 1524, 2416, 2978, 3781, 4702
NsiI ATGCAT 7 cut(s) 970, 1300, 1674, 2395, 2907, 3226, 5189
OliI CACNNNNGTG 3 cut(s) 620, 3196, 5970
PaeI GCATGC 3 cut(s) 2909, 4864, 5726
PagI TCATGA 3 cut(s) 3045, 3693, 5224
PceI AGGCCT 1 cut(s) 3899
PciI ACATGT 2 cut(s) 333, 684
PciSI GCTCTTC 2 cut(s) 3875, 6126
PctI GAATGC 4 cut(s) 1300, 2324, 3844, 5981
PdmI GAANNNNTTC 2 cut(s) 3260, 5473
PflFI GACNNNGTC 1 cut(s) 1060
PflMI CCANNNNNTGG 2 cut(s) 1174, 2616
PfoI TCCNGGA 2 cut(s) 1510, 5668
PleI GAGTC 8 cut(s) 499, 1476, 2413, 2612, 3027, 4730, 5619, 6104
PmaCI CACGTG 1 cut(s) 3466
PmlI CACGTG 1 cut(s) 3466
PpsI GAGTC 8 cut(s) 499, 1476, 2413, 2612, 3027, 4730, 5619, 6104
Ppu21I YACGTR 4 cut(s) 444, 1687, 3466, 5768
PpuMI RGGWCCY 1 cut(s) 6335
PscI ACATGT 2 cut(s) 333, 684
PshBI ATTAAT 3 cut(s) 1994, 2067, 2462
PsiI TTATAA 2 cut(s) 695, 1743
Psp124BI GAGCTC 1 cut(s) 3870
Psp5II RGGWCCY 1 cut(s) 6335
PspCI CACGTG 1 cut(s) 3466
PspFI CCCAGC 3 cut(s) 462, 4016, 5289
PspOMI GGGCCC 2 cut(s) 1606, 5172
PspPPI RGGWCCY 1 cut(s) 6335
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PstI CTGCAG 5 cut(s) 2719, 4683, 4696, 5383, 5610
PstNI CAGNNNCTG 2 cut(s) 2264, 2297
PsuI RGATCY 5 cut(s) 366, 3003, 4272, 5665, 5740
PsyI GACNNNGTC 1 cut(s) 1060
PvuII CAGCTG 1 cut(s) 2876
RseI CAYNNNNRTG 8 cut(s) 620, 1002, 2079, 3196, 3385, 4661, 5684, 5970
SacI GAGCTC 1 cut(s) 3870
SapI GCTCTTC 2 cut(s) 3875, 6126
ScaI AGTACT 1 cut(s) 1027
SchI GAGTC 8 cut(s) 500, 1477, 2414, 2613, 3027, 4730, 5620, 6105
SexAI ACCWGGT 1 cut(s) 6262
SfcI CTRYAG 8 cut(s) 2005, 2265, 2715, 4679, 4692, 5162, 5379, 5606
SinI GGWCC 7 cut(s) 71, 188, 2501, 2866, 5026, 6161, 6335
SmiMI CAYNNNNRTG 8 cut(s) 620, 1002, 2079, 3196, 3385, 4661, 5684, 5970
SmlI CTYRAG 7 cut(s) 797, 872, 1568, 3583, 3655, 3901, 4148
SmoI CTYRAG 7 cut(s) 797, 872, 1568, 3583, 3655, 3901, 4148
SnaBI TACGTA 2 cut(s) 444, 5768
SpeI ACTAGT 1 cut(s) 1499
SphI GCATGC 3 cut(s) 2909, 4864, 5726
SseBI AGGCCT 1 cut(s) 3899
SspI AATATT 5 cut(s) 1747, 2963, 3090, 3217, 5297
SstI GAGCTC 1 cut(s) 3870
StuI AGGCCT 1 cut(s) 3899
StyI CCWWGG 3 cut(s) 191, 718, 5176
TaqI TCGA 7 cut(s) 281, 2638, 3801, 3943, 4410, 4451, 5394
TaqII GACCGA 1 cut(s) 1651
TatI WGTACW 5 cut(s) 612, 1025, 1543, 1654, 2806
TauI GCSGC 1 cut(s) 3822
TseFI GTSAC 6 cut(s) 336, 1524, 2416, 2978, 3781, 4702
Tsp45I GTSAC 6 cut(s) 336, 1524, 2416, 2978, 3781, 4702
TspGWI ACGGA 1 cut(s) 541
Tth111I GACNNNGTC 1 cut(s) 1060
Van91I CCANNNNNTGG 2 cut(s) 1174, 2616
Vha464I CTTAAG 2 cut(s) 3583, 3901
VneI GTGCAC 1 cut(s) 5425
VpaK11BI GGWCC 7 cut(s) 71, 188, 2501, 2866, 5026, 6161, 6335
VspI ATTAAT 3 cut(s) 1994, 2067, 2462
XagI CCTNNNNNAGG 1 cut(s) 2749
XcmI CCANNNNNNNNNTGG 1 cut(s) 3192
XmaJI CCTAGG 1 cut(s) 5176
XmiI GTMKAC 3 cut(s) 2645, 5049, 5770
XmnI GAANNNNTTC 2 cut(s) 3260, 5473
ZrmI AGTACT 1 cut(s) 1027
Zsp2I ATGCAT 7 cut(s) 970, 1300, 1674, 2395, 2907, 3226, 5189
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.