Rw6G002430

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000376
Physical Location & Seq
Reverse (-)
3956702 .. 3965538
8837 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002430.1

Sequence Viewer

Length: 6180 bp
ATGGCCGATTCTGGAGCTAACCCTAAACAGAGACAACCCAAGAAGAACAAGAAGAAGAGAAAGCAGAGCGGACCCCAAGAATCTGAAAGGCCACCCAAGACTAACCGCGCCAATTCATCAGAGAACGAGAATGAACCACAAGACCAATACTTTGAAGTTGGAAAAGCTGAAAAACCCAATCAAGGTGGTCCATGGAGGAACCTGGAGTTAATTCTGTCAATCCAGAACAGAGAGCTTGATCTTCACAAGAAGGTGGAACTAGCTCATGGTTTTGTGATGTCGAGGGTGAAGGAAGAAGGAGGCAGCAGTGATGAGGATAATCAAGCAGTGAACATGTCACGTTTAGTAATTTTTGTCAGTGATTGGGTTCAATCATTGTTGATTTCCGAAGGAAAGAAAGTTGAGAGTGGTGGAGAGAAGCATCAAGCTGAAGTTGTGTATACGTATTTGGATTTAAGATGCTGGGAGATTTTCAAATTCTGTTTGGAGGAGTCTCTGAAATTAAATGTTTCTTTAACTTTCTCACGGAACCTTTTACGCTCTATATGTTGGATTGCAAGAAGTGCACTGTCCTTATTGAACAAAACATTTATTGGTGAAGGGTTACAATTGTACACTACTGTGCTTGATTGTATTTCTTTGGTATTTTCATCCCAAGAAGGCTTCTCAAATGAAAATTTAGACATGTGGATTACAACTGTAAGTTCAGTTCTTGACCTTGGGCACAAATGTTACTGTGAAGCTCCTGTTAGTGGCAATGAGGGTGTATTTGTTTTCCAATTCTTGTGCTTGGTGCTTGAGCCATTTGCCAGGTTTTTAAGGGCTCACGCTCGGAAAGATGGATTTCGTGATTTTAGTAATAAGCTCCTTGAGCCTCTGTTGCATCTCTTGGGTCTCTTGCATCTCCAGATTGGTGGAAGTAATCCTGGTTGGACAAGAAACATACTGAAACTTGTTGAAGATGTATTATCTCATGGGCTATATCACCCGCTCCACATTGATGGGTTTTTGAGCTTATGCAGTACTGAAAAAGACGCCACATCCAATGATGGGAAGTCAAAAGATTCCAAAACAATCAACCAGAGTTATCATAGACATTTATTTGATAAAGTGGAAGGAATTTTAGCTGCAAAGAATGCATTTGCAGTAGAAAGCATAGGAGTATTGTTTCACTTGCTTATTGATCAAGTTAAAAAGCTAAAGAGGGATTCAGCGCTGACTGCAAGTACCAAAATGATGGGGAAGAGTGAAGGTTCAAGGCAGTTAGAAGATAGTTCATTGGGTCAAACGTCTATGATGTCTTCTGAGAGTCGTATTGTTGAAAAGAATTATTGCTCAACTAGTTTCAATCCAGGAACACGAAAGTCACTTCACGATTTTTTTGTACTGATTATGGAGCCTCTTTTGCTTGAGATCAATGGCTTTCTTGAAACTAAGCTGAAAGTGGGCCCTGAATTGTTGGATATTCATTGCACTCTTAAAGCTATAAATAATGTACTTTCGGTCTTTATGCATGAAAAGATTTATGTGAGAACAGAGGATGCCTCTGAGGGAGCTTCTCTTAATTTCTTGAAGAAGGTTTATAATATTATCATCTCATTGTCCTCAGATTTAATTCAGTTGTCAGTTATACACTCAGATAATTTTACTCTAATAGCAAATGAGGTACTTTCTGCTGTTGGCTATCTTGTGGAAATTGAATATGATGTTCTAGAGAATGATCTAGTAAGCTTGTGGTTAATGATCCTCTCTTACTGGGCCATTGGGCTTTCTTTGGTGGATTCTCCGGATAGATGCTCAATATTCTCCAAGATAACAGATATTGGATGCCAATTAATTATGCTCTATAGTCAACTTCGCCAGGTGAATAATACTATCTTTGCATTGTGTAAAGCAATAAGGGTTATTAATTTACATAATAGCGATGGTGAACTGAACTATACTAGATTTGTCATTCCTTTTCATGGTGAAGCATATGCAAAAGCTGTGGAAATGCTATTATCTGCACACGAATTCAAAATTGCAATTCATAAAGCAATGAAGTCCATACCAGAAGGACAAGCAAGTCAATGTATTCAGCAGTTAACATTGGATATATCAGAATCTCTAGAGTGGATGAAAGTTAGTTGTTCGGAGGCTGATGAAAAAGAATTTTCTGAATGCCATCTAAGTAGCTTGCACAATTTTAATCTGGAAGCTGAACTTTTCGGGAGAGGGTTGTCAGAAATATATGCATTATTCCTAGAGTCATTGATTGTCACAACTGGAAACTGTAACCTTCTTGGCGCTTCTATAAAGGAATTAATTAGAGTGATTTTCCCTTGCATGAGCAATCTGGTTGGACCACAGCAGGAGGATGCTGTAAACAAATTCCTGTGGTCTGTCGCGGTAAAAGATTCTGACAATGTGATCACTAGAAACAAAAACAAATATATGATATTTGGAGTCTCCACTTATTGGGTAGTTTTGTTCTTCTTTCGATTGTATAGCTCCTGCCGATGCTTATATAGGCAAGCAGCAATCCTTATGCCTCCAGATTTATCAAGAAAGATGTCTGCAGAAATGGGAGATTCGTTCTCTTCATTCTCTGGCAGGGATTGGATGGAAATGTCTGATTGGATTAATGGGGGATTTTTCTCTTGGATTGTACAACCTTCAGCTTCTCTTTTTTCTGTTATTCAATCTGTTTCAAACATCTTTTGTAAGGACCGTGCAGCTGATTCTTGTCCGTTAACTTATCTATATTTGAATAGTCGTATAAAGTCTTTTGAATATTTGATTGAAAATGGTGACAATCTGGTGCAAGTTGCGGACATCTCTTCTTTGAGGCAAGAGGCAGCAGGACTCACGGGATTTATGATGGGGCACCTCTCATTAGTGTCTGAAGATCAACAGCGAATATTCATTTCTGCTGATACGACTAATAATAAGATGGTTGACTATGAAAGTGATGAATGGGATTTCAGTATTTGCTCTGTTAACAAGAAGTCATTGCCAACTGCACTTTGGTGGGTAGTTTGCCAAAATATTCATGCATGGTGCCCTCATGCCTCTAAAAAGGATTTGAAGAGATTCCTTTCTCTTTTAATCCATACTTCAATTCCCTATGTAAGAAGCAGCTTTGGGGTGGTTATAGAGCACAAGAACCATGAAGCTGACAGGCTGAAGAATGTGGCATTGCATCAAATCTCATCACAGTGCTTTATCGATTCCAGTCTGTATGAGCAAAGATTTGTCCGCAGGTATTTTGCAAAGAGGTTCTGCCGTGCATTGGAGAAATCTACATTACCATTCATCGGCGACTTTCCATCCGGCAATGTTAAATTCAAATCATCACCTAATTGGCCAAATGTCTTAAGTGATTTAGAGAACTCATCATTGGCTATTTCCTGCAACAAACTCAATGTATTTGATTGTTCCTCAGCCTCAAGTTGCAAGGGACAAAACTCCCAACCATCTACCATCATGAAATTTACAGCTTGTCAGAGTTTGCTTAATCTTTTGAGTTGCATGCCAAAAGGTCATTTGGACACGAGATCATTTTCACGTTATGTGACTTCTATTCTGAACCTTGAAAGGATTGTTGTTGGCGGCTTATTAGATTATCAGAATGCTTCATACTCAACTTATTATTATGAGCTCTTCAGATTGTTTGTGTCCTGTCGGAAGGCCTTACGATGTATAATTACTGCTTGTGAGGAGACAGTAGCCGGTCGAACCTCAGACACTGTACTCTTTGAGGATTTATTTCCAGTTTCATGGCTTTACAAGTCAGTGCATATGGTTGCTGGGCTTCAAGAATCATTCTCAAAAGATATTTATCATCAAGTTCATGATATGATCTTGGCATTAATGGATCACACATTTTATGTGTTTCTGACATTAAATAAATATCAATCCAATCATGCTGTTTGTTTCCTTGAGGTTGCTGAGCTGAATTTTGGATGTGCCCAAGAACAGAGAAGTTTATTGAATTCGTCCAATTATATTGAAGCCTGGAAAAGTGTAAACGATGTTGCTAAGATTTTGAAGGAACAGATGCAGATCTTACTTGTAAATGTGAAGAATGGCATTTTTAATGGAAAAGAGGAAGTTTCTGTTGATGCTTTAAATTTGAACAAGTTTGCTTCCATAATTTCGTGTTTTAGTGGGTTTTTATGGGGCCTAGCGTGTGTTGTGATCGACACAGATGGGAGAAATTGTGATGAGAAAGCTAAGTTGTCAAGGTTGAAACTTGAACCAATCTCCGAATTCAACCTCTGCATAAAGGTTTTTGAAGAAATTTCTAGTCTTCTACTACAAATGTTTATTCTTGACGATAATCAGCAGTCCACAACTATATGTCATGCCTATAATCTTCAGAAGTCGGGCTACAATTTAGATTTTTTGGGTGCAGAGAAGATTTTTCCGGAAGGCGATGATGCTGACACTGATATGGCATGTGGTGGACTGCAGGATGAATCTGCAGTTGCAGTGACGTGCTCAGCATCATCTGACATCTGTGAAGACTCTGTAATTGGTAGTGTTCATAGAAGAAGACCGCGCTTGAAAGATGCAAATAGTGTTGTTACTGTTCTCACTGCTGTTGATTCATTTGAACTGCAATCTTTAAATAAGCCTTTGTTGAGAAGCATGCTAAAAGGTGATTTCCCCAACGCAGCATTCTTACTGAGGCAGTTGTTAATTGCCTCTTCTGCTATTTTAAGGCTAAATTTGCATATTAAGAGCGCTCCCATGTTGTCAAGCTTGGTGCATAAGTTTGCTGGCATTATGCAAGTCTTGTTATTGGAATCAGCGGACACAAGTCAGGTGCCACACTTCTATTATTTTGTGTGTTTAGATGGTGTTCTGAAGTATCTGGGAGAATTAGGGAATCATTTTCCATTGACTAATCCTACCTTATCCAGAGATTTGTTTGACAAGATGGTTCAGCTACAGTTACGGGCTCTAGGGAAATGCATAACTTTACAAGGGAAAAGAGCAACTCTTGCATCTCATGAAACAAGCACCAACACACCCTTAGGAGAAAGGAGATTTTCTGAAGCATCATCTTTTTCTGGCTGGGAATATTTATTGGATGAATTCAAAGCTAGGTTGAGGTCATCATTTGCAGTGTTCATAAATAAATCAACAGAGTTGCATCTCCAATCTGCAGTTCAAGCTATCGAAAGAGCCCTAGTTGGTGTGCGGGAAGGGTGCACAGTGAGGTATGACATATGTGTTGGAAGTGAAGATGGAGGAAAGGTTTCTTCAATTGTTGCATCTGGCATTGATTGCTTGGATTTGGTTCTGGAATTTGTTTCAGGACGCAATTTGATTGTGGTTAAAAAATACATTCAGAGGTTAATTGCCTGCATGTTCAATGTTATTCTGCATCTGCAGAGTCCACTAATCTTCTATGAGAGATTAACTATGAGTAAAGATGATAGTGATCCAGATCCAGGAACAGTCATTCTTATGTGTGTTGATGTACTGGCAAGAATTTCTGGCAAGCATGCAATATACAAAATGGATCTCTGGCATGTAGCGCACTCGTTACGTATACCTTCAGCACTTTTTGAAGATTTCCATCTGTTAAAACATTCTAAATGTCCTGTTCCAAATGATTCTTCAACAAGTCCAAATAACCAACCATCCGATCCTGTAGCAAGTGTACATGTCTCTGGCATAGATAGGCAATACTCAATCGGTCTATATTCTGCTTGCTGCCGATTACTGCATAATGTTGTGAAGCATCACAAAAGTGAATGCGAGGGGTATGTTGCTCTACTTCAAGCTTCTGTTCGTGTTCTTCTTTATTGTTTGGAGACATTGGATGCTGTTGTGGTAGCCAGGGAAGGCTTCTTTTCTTGGGAAGTAGAAGAAGGAGTCAAATGTGCATGTTCTCTCAGAAGGATTTATGAAGAGCTAAGACATCAAAAAGAGGTCTTTGGTCCACACTGTTACCACTTTTTAGCTTATTACATACGGGTTTATTCAGGATATGGCCCCCGCAAAACAGGCATCAAAAGGGAAATAGATGAAGCTCTAAGACCAGGTGTATATGCGCTGATAGATGTTTGCTCACCTGATGATCTTCAACGTCTTCATACCTCATTTGGAGAGGGTCCTTGCAGAAACACCTTGGCAACCTTGAAGCACGATTATGTACTCAATTTCCAGTATCAGGGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

2059

Amino Acids

231.77

Weight (kDa)

6.25

Isoelectric Point (pI)

45.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1820 - 2058 2.1e-37 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1584
AasI GACNNNNNNGTC 1 cut(s) 2064
Acc36I ACCTGC 1 cut(s) 3231
AccB1I GGYRCC 3 cut(s) 2865, 3039, 4810
AccB7I CCANNNNNTGG 1 cut(s) 2457
AccBSI CCGCTC 2 cut(s) 69, 991
AccI GTMKAC 2 cut(s) 440, 5542
AccII CGCG 3 cut(s) 108, 2387, 4543
AccIII TCCGGA 2 cut(s) 1786, 4408
AclWI GGATC 6 cut(s) 1738, 3864, 5426, 5432, 5520, 5633
AcoI YGGCCR 2 cut(s) 3, 3344
AcuI CTGAAG 9 cut(s) 450, 2640, 2904, 3185, 3628, 4343, 4871, 5061, 5532
AcyI GRCGYC 1 cut(s) 1035
AfeI AGCGCT 2 cut(s) 1215, 4729
AfiI CCNNNNNNNGG 9 cut(s) 182, 752, 1050, 1964, 2457, 3271, 3296, 5441, 6166
AflII CTTAAG 1 cut(s) 3355
AflIII ACRYGT 3 cut(s) 333, 684, 5656
AhlI ACTAGT 1 cut(s) 1340
AjiI CACGTC 1 cut(s) 4479
AjnI CCWGG 9 cut(s) 201, 809, 925, 1351, 1860, 3995, 5440, 5831, 6034
AjuI GAANNNNNNNTTGG 8 cut(s) 3362, 3394, 4646, 4678, 5139, 5171, 5593, 5625
AleI CACNNNNGTG 3 cut(s) 620, 3007, 5742
Alw21I GWGCWC 5 cut(s) 568, 3141, 3642, 4484, 5201
Alw26I GTCTC 7 cut(s) 25, 498, 899, 2452, 3695, 5665, 5801
Alw44I GTGCAC 2 cut(s) 564, 5197
AlwI GGATC 6 cut(s) 1738, 3864, 5426, 5432, 5520, 5633
AlwNI CAGNNNCTG 1 cut(s) 3728
Aor13HI TCCGGA 2 cut(s) 1786, 4408
Aor51HI AGCGCT 2 cut(s) 1215, 4729
AoxI GGCC 8 cut(s) 3, 89, 1447, 1758, 3344, 3669, 4162, 5986
ApaI GGGCCC 1 cut(s) 1451
ApaLI GTGCAC 2 cut(s) 564, 5197
ApeKI GCWGC 8 cut(s) 303, 1127, 2516, 2714, 2837, 3117, 4658, 5706
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AseI ATTAAT 5 cut(s) 1835, 1908, 2303, 2622, 3851
Asp700I GAANNNNTTC 2 cut(s) 3071, 5245
AspLEI GCGC 7 cut(s) 110, 1216, 2288, 4545, 4730, 5530, 6049
AvaII GGWCC 6 cut(s) 71, 188, 2342, 2707, 5933, 6107
AxyI CCTNAGG 1 cut(s) 5020
BaeGI GKGCMC 7 cut(s) 568, 726, 1451, 2868, 3044, 3952, 5201
BalI TGGCCA 1 cut(s) 3346
BanI GGYRCC 3 cut(s) 2865, 3039, 4810
BanII GRGCYC 5 cut(s) 826, 1451, 3642, 4948, 5176
BarI GAAGNNNNNNTAC 2 cut(s) 4355, 4387
BauI CACGAG 1 cut(s) 3532
BbsI GAAGAC 5 cut(s) 1293, 4283, 4512, 4543, 6077
Bbv12I GWGCWC 5 cut(s) 568, 3141, 3642, 4484, 5201
BbvCI CCTCAGC 1 cut(s) 3421
BbvI GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2849, 3129, 4670, 5693
BceAI ACGGC 1 cut(s) 3249
BciT130I CCWGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
BclI TGATCA 2 cut(s) 1183, 2409
BcoDI GTCTC 7 cut(s) 25, 498, 899, 2452, 3695, 5665, 5801
BcuI ACTAGT 1 cut(s) 1340
BfmI CTRYAG 8 cut(s) 1846, 2556, 4451, 4464, 4934, 5151, 5378, 5643
BfoI RGCGCY 3 cut(s) 1217, 2289, 4731
BfrI CTTAAG 1 cut(s) 3355
BfuAI ACCTGC 1 cut(s) 3231
BglII AGATCT 1 cut(s) 4044
BisI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2838, 3118, 3592, 4659, 5707
BlpI GCTNAGC 2 cut(s) 3930, 4483
BlsI GCNGC 9 cut(s) 305, 1129, 2518, 2716, 2839, 3119, 3593, 4660, 5708
BmcAI AGTACT 1 cut(s) 1024
Bme1390I CCNGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
Bme18I GGWCC 6 cut(s) 71, 188, 2342, 2707, 5933, 6107
BmgBI CACGTC 1 cut(s) 4479
BmrFI CCNGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
BmrI ACTGGG 1 cut(s) 1765
BmuI ACTGGG 1 cut(s) 1765
BoxI GACNNNNGTC 1 cut(s) 4803
BpiI GAAGAC 5 cut(s) 1293, 4283, 4512, 4543, 6077
BplI GAGNNNNNCTC 2 cut(s) 1529, 1561
BpmI CTGGAG 4 cut(s) 33, 224, 890, 2517
Bpu10I CCTNAGC 1 cut(s) 3421
Bpu1102I GCTNAGC 2 cut(s) 3930, 4483
BpuEI CTTGAG 5 cut(s) 818, 890, 1430, 3412, 3941
Bsa29I ATCGAT 1 cut(s) 3207
BsaAI YACGTR 2 cut(s) 444, 5540
BsaBI GATNNNNATC 5 cut(s) 3819, 4043, 5430, 5436, 5568
BsaHI GRCGYC 1 cut(s) 1035
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 4 cut(s) 191, 718, 5832, 6123
BsaWI WCCGGW 2 cut(s) 1786, 4408
BsaXI ACNNNNNCTCC 4 cut(s) 4186, 4216, 5229, 5259
Bsc4I CCNNNNNNNGG 9 cut(s) 182, 752, 1050, 1964, 2457, 3271, 3296, 5441, 6166
Bse118I RCCGGY 1 cut(s) 3710
Bse1I ACTGG 6 cut(s) 1760, 2269, 3213, 3752, 5478, 6160
Bse21I CCTNAGG 1 cut(s) 5020
Bse3DI GCAATG 6 cut(s) 763, 1468, 2043, 2990, 3176, 3322
Bse8I GATNNNNATC 5 cut(s) 3819, 4043, 5430, 5436, 5568
BseAI TCCGGA 2 cut(s) 1786, 4408
BseBI CCWGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
BseCI ATCGAT 1 cut(s) 3207
BseDI CCNNGG 4 cut(s) 191, 718, 5832, 6123
BseJI GATNNNNATC 5 cut(s) 3819, 4043, 5430, 5436, 5568
BseLI CCNNNNNNNGG 9 cut(s) 182, 752, 1050, 1964, 2457, 3271, 3296, 5441, 6166
BseMI GCAATG 6 cut(s) 763, 1468, 2043, 2990, 3176, 3322
BseNI ACTGG 6 cut(s) 1760, 2269, 3213, 3752, 5478, 6160
BseRI GAGGAG 2 cut(s) 503, 3713
BseSI GKGCMC 7 cut(s) 568, 726, 1451, 2868, 3044, 3952, 5201
BseXI GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2849, 3129, 4670, 5693
BseYI CCCAGC 3 cut(s) 462, 3788, 5061
BsgI GTGCAG 4 cut(s) 1989, 2733, 2985, 4413
Bsh1236I CGCG 3 cut(s) 108, 2387, 4543
Bsh1285I CGRYCG 1 cut(s) 3715
BshFI GGCC 8 cut(s) 5, 91, 1449, 1760, 3346, 3671, 4164, 5988
BshNI GGYRCC 3 cut(s) 2865, 3039, 4810
BshVI ATCGAT 1 cut(s) 3207
BsiEI CGRYCG 1 cut(s) 3715
BsiHKAI GWGCWC 5 cut(s) 568, 3141, 3642, 4484, 5201
BsiSI CCGG 4 cut(s) 1787, 3312, 3711, 4409
BslFI GGGAC 1 cut(s) 3453
BslI CCNNNNNNNGG 9 cut(s) 182, 752, 1050, 1964, 2457, 3271, 3296, 5441, 6166
BsmAI GTCTC 7 cut(s) 25, 498, 899, 2452, 3695, 5665, 5801
BsmFI GGGAC 1 cut(s) 3453
BsmI GAATGC 5 cut(s) 1141, 2165, 3616, 4661, 5753
BsnI GGCC 8 cut(s) 5, 91, 1449, 1760, 3346, 3671, 4164, 5988
Bso31I GGTCTC 1 cut(s) 899
Bsp120I GGGCCC 1 cut(s) 1447
Bsp13I TCCGGA 2 cut(s) 1786, 4408
Bsp1407I TGTACA 3 cut(s) 612, 2647, 5653
Bsp1720I GCTNAGC 2 cut(s) 3930, 4483
Bsp19I CCATGG 1 cut(s) 191
BspANI GGCC 8 cut(s) 5, 91, 1449, 1760, 3346, 3671, 4164, 5988
BspDI ATCGAT 1 cut(s) 3207
BspEI TCCGGA 2 cut(s) 1786, 4408
BspFNI CGCG 3 cut(s) 108, 2387, 4543
BspHI TCATGA 3 cut(s) 3465, 3832, 4996
BspMAI CTGCAG 5 cut(s) 2560, 4455, 4468, 5155, 5382
BspMI ACCTGC 1 cut(s) 3231
BspPI GGATC 6 cut(s) 1738, 3864, 5426, 5432, 5520, 5633
BspQI GCTCTTC 2 cut(s) 3647, 5898
BspT107I GGYRCC 3 cut(s) 2865, 3039, 4810
BspTI CTTAAG 1 cut(s) 3355
BspTNI GGTCTC 1 cut(s) 899
BsrBI CCGCTC 2 cut(s) 69, 991
BsrDI GCAATG 6 cut(s) 763, 1468, 2043, 2990, 3176, 3322
BsrFI RCCGGY 1 cut(s) 3710
BsrGI TGTACA 3 cut(s) 612, 2647, 5653
BsrI ACTGG 6 cut(s) 1760, 2269, 3213, 3752, 5478, 6160
BssAI RCCGGY 1 cut(s) 3710
BssECI CCNNGG 4 cut(s) 191, 718, 5832, 6123
BssNAI GTATAC 2 cut(s) 441, 5543
BssNI GRCGYC 1 cut(s) 1035
BssSI CACGAG 1 cut(s) 3532
BssT1I CCWWGG 3 cut(s) 191, 718, 6123
Bst1107I GTATAC 2 cut(s) 441, 5543
Bst2BI CACGAG 1 cut(s) 3532
Bst2UI CCWGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
Bst6I CTCTTC 8 cut(s) 50, 1238, 2584, 2824, 3062, 3647, 4696, 5898
BstACI GRCGYC 1 cut(s) 1035
BstAFI CTTAAG 1 cut(s) 3355
BstAPI GCANNNNNTGC 4 cut(s) 563, 1136, 4988, 5274
BstAUI TGTACA 3 cut(s) 612, 2647, 5653
BstBAI YACGTR 2 cut(s) 444, 5540
BstC8I GCNNGC 9 cut(s) 2177, 2514, 3512, 4634, 4765, 5353, 5492, 5496, 5704
BstDSI CCRYGG 1 cut(s) 191
BstFNI CGCG 3 cut(s) 108, 2387, 4543
BstH2I RGCGCY 3 cut(s) 1217, 2289, 4731
BstHHI GCGC 7 cut(s) 110, 1216, 2288, 4545, 4730, 5530, 6049
BstMAI GTCTC 7 cut(s) 25, 498, 899, 2452, 3695, 5665, 5801
BstMCI CGRYCG 1 cut(s) 3715
BstNI CCWGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
BstPAI GACNNNNGTC 1 cut(s) 4803
BstSCI CCNGG 9 cut(s) 201, 809, 925, 1351, 1860, 3995, 5440, 5831, 6034
BstSFI CTRYAG 8 cut(s) 1846, 2556, 4451, 4464, 4934, 5151, 5378, 5643
BstSLI GKGCMC 7 cut(s) 568, 726, 1451, 2868, 3044, 3952, 5201
BstSNI TACGTA 2 cut(s) 444, 5540
BstUI CGCG 3 cut(s) 108, 2387, 4543
BstV1I GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2849, 3129, 4670, 5693
BstV2I GAAGAC 5 cut(s) 1293, 4283, 4512, 4543, 6077
BstX2I RGATCY 3 cut(s) 4044, 5437, 5512
BstXI CCANNNNNNTGG 4 cut(s) 914, 1001, 1237, 3759
BstYI RGATCY 3 cut(s) 4044, 5437, 5512
BstZ17I GTATAC 2 cut(s) 441, 5543
Bsu15I ATCGAT 1 cut(s) 3207
Bsu36I CCTNAGG 1 cut(s) 5020
BsuRI GGCC 8 cut(s) 5, 91, 1449, 1760, 3346, 3671, 4164, 5988
BsuTUI ATCGAT 1 cut(s) 3207
BtgI CCRYGG 1 cut(s) 191
BtgZI GCGATG 2 cut(s) 1938, 4431
BtrI CACGTC 1 cut(s) 4479
BtsI GCAGTG 5 cut(s) 313, 333, 4479, 4578, 5118
BveI ACCTGC 1 cut(s) 3231
Cac8I GCNNGC 9 cut(s) 2177, 2514, 3512, 4634, 4765, 5353, 5492, 5496, 5704
CaiI CAGNNNCTG 1 cut(s) 3728
CciI TCATGA 3 cut(s) 3465, 3832, 4996
CfoI GCGC 7 cut(s) 110, 1216, 2288, 4545, 4730, 5530, 6049
Cfr10I RCCGGY 1 cut(s) 3710
ClaI ATCGAT 1 cut(s) 3207
CseI GACGC 2 cut(s) 1043, 5316
CsiI ACCWGGT 1 cut(s) 6034
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 1968, 2003
DraI TTTAAA 2 cut(s) 4110, 4611
DrdI GACNNNNNNGTC 1 cut(s) 2064
DseDI GACNNNNNNGTC 1 cut(s) 2064
EaeI YGGCCR 2 cut(s) 3, 3344
Eam1104I CTCTTC 8 cut(s) 50, 1238, 2584, 2824, 3062, 3647, 4696, 5898
EarI CTCTTC 8 cut(s) 50, 1238, 2584, 2824, 3062, 3647, 4696, 5898
Ecl136II GAGCTC 1 cut(s) 3640
Eco105I TACGTA 2 cut(s) 444, 5540
Eco130I CCWWGG 3 cut(s) 191, 718, 6123
Eco147I AGGCCT 1 cut(s) 3671
Eco24I GRGCYC 5 cut(s) 826, 1451, 3642, 4948, 5176
Eco31I GGTCTC 1 cut(s) 899
Eco47I GGWCC 6 cut(s) 71, 188, 2342, 2707, 5933, 6107
Eco47III AGCGCT 2 cut(s) 1215, 4729
Eco53kI GAGCTC 1 cut(s) 3640
Eco57I CTGAAG 9 cut(s) 450, 2640, 2904, 3185, 3628, 4343, 4871, 5061, 5532
Eco81I CCTNAGG 1 cut(s) 5020
EcoICRI GAGCTC 1 cut(s) 3640
EcoO109I RGGNCCY 3 cut(s) 1448, 4162, 6107
EcoRI GAATTC 4 cut(s) 2012, 3973, 4250, 5081
EcoRII CCWGG 9 cut(s) 201, 809, 925, 1351, 1860, 3995, 5440, 5831, 6034
EcoT14I CCWWGG 3 cut(s) 191, 718, 6123
EcoT22I ATGCAT 5 cut(s) 1141, 1515, 2236, 3037, 4961
EcoT38I GRGCYC 5 cut(s) 826, 1451, 3642, 4948, 5176
ErhI CCWWGG 3 cut(s) 191, 718, 6123
FalI AAGNNNNNCTT 2 cut(s) 2187, 2219
FaqI GGGAC 1 cut(s) 3453
FauI CCCGC 3 cut(s) 996, 5181, 5999
FauNDI CATATG 3 cut(s) 1975, 3780, 5216
FbaI TGATCA 2 cut(s) 1183, 2409
FblI GTMKAC 2 cut(s) 440, 5542
Fnu4HI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2838, 3118, 3592, 4659, 5707
FriOI GRGCYC 5 cut(s) 826, 1451, 3642, 4948, 5176
Fsp4HI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2838, 3118, 3592, 4659, 5707
GlaI GCGC 7 cut(s) 109, 1215, 2287, 4544, 4729, 5529, 6048
GluI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2838, 3118, 3592, 4659, 5707
GsaI CCCAGC 3 cut(s) 466, 3792, 5065
GsuI CTGGAG 4 cut(s) 33, 224, 890, 2517
HaeII RGCGCY 3 cut(s) 1217, 2289, 4731
HaeIII GGCC 8 cut(s) 5, 91, 1449, 1760, 3346, 3671, 4164, 5988
HapII CCGG 4 cut(s) 1787, 3312, 3711, 4409
HgaI GACGC 2 cut(s) 1043, 5316
HhaI GCGC 7 cut(s) 110, 1216, 2288, 4545, 4730, 5530, 6049
Hin1I GRCGYC 1 cut(s) 1035
Hin6I GCGC 7 cut(s) 108, 1214, 2286, 4543, 4728, 5528, 6047
HinP1I GCGC 7 cut(s) 108, 1214, 2286, 4543, 4728, 5528, 6047
HincII GTYRAC 5 cut(s) 1853, 2085, 2733, 2938, 2980
HindII GTYRAC 5 cut(s) 1853, 2085, 2733, 2938, 2980
HindIII AAGCTT 3 cut(s) 1729, 4744, 5775
HpaI GTTAAC 3 cut(s) 2085, 2733, 2980
HpaII CCGG 4 cut(s) 1787, 3312, 3711, 4409
HpyCH4IV ACGT 7 cut(s) 340, 443, 1289, 3547, 4478, 5539, 6082
HpySE526I ACGT 7 cut(s) 340, 443, 1289, 3547, 4478, 5539, 6082
Hsp92I GRCGYC 1 cut(s) 1035
HspAI GCGC 7 cut(s) 108, 1214, 2286, 4543, 4728, 5528, 6047
Kpn2I TCCGGA 2 cut(s) 1786, 4408
Ksp22I TGATCA 2 cut(s) 1183, 2409
KspAI GTTAAC 3 cut(s) 2085, 2733, 2980
LguI GCTCTTC 2 cut(s) 3647, 5898
LmnI GCTCC 8 cut(s) 14, 748, 870, 996, 1396, 1553, 2495, 4735
Lsp1109I GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2849, 3129, 4670, 5693
MabI ACCWGGT 1 cut(s) 6034
MaeII ACGT 7 cut(s) 340, 443, 1289, 3547, 4478, 5539, 6082
MbiI CCGCTC 2 cut(s) 69, 991
MfeI CAATTG 2 cut(s) 608, 5253
MflI RGATCY 3 cut(s) 4044, 5437, 5512
MlsI TGGCCA 1 cut(s) 3346
MluNI TGGCCA 1 cut(s) 3346
MlyI GAGTC 8 cut(s) 500, 1318, 2255, 2454, 2838, 4502, 5392, 5877
MmeI TCCRAC 7 cut(s) 139, 530, 911, 1440, 2320, 3644, 5203
Mox20I TGGCCA 1 cut(s) 3346
Mph1103I ATGCAT 5 cut(s) 1141, 1515, 2236, 3037, 4961
MroI TCCGGA 2 cut(s) 1786, 4408
MroXI GAANNNNTTC 2 cut(s) 3071, 5245
MscI TGGCCA 1 cut(s) 3346
MslI CAYNNNNRTG 8 cut(s) 620, 999, 3007, 3196, 4433, 5456, 5742, 6144
Msp20I TGGCCA 1 cut(s) 3346
MspA1I CMGCKG 2 cut(s) 2717, 4796
MspCI CTTAAG 1 cut(s) 3355
MspI CCGG 4 cut(s) 1787, 3312, 3711, 4409
MspR9I CCNGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
MunI CAATTG 2 cut(s) 608, 5253
Mva1269I GAATGC 5 cut(s) 1141, 2165, 3616, 4661, 5753
MvaI CCWGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
MvnI CGCG 3 cut(s) 108, 2387, 4543
NcoI CCATGG 1 cut(s) 191
NdeI CATATG 3 cut(s) 1975, 3780, 5216
NmuCI GTSAC 6 cut(s) 336, 1365, 2257, 2789, 3553, 4474
NsiI ATGCAT 5 cut(s) 1141, 1515, 2236, 3037, 4961
OliI CACNNNNGTG 3 cut(s) 620, 3007, 5742
PaeI GCATGC 3 cut(s) 3514, 4636, 5498
PagI TCATGA 3 cut(s) 3465, 3832, 4996
PceI AGGCCT 1 cut(s) 3671
PciI ACATGT 3 cut(s) 333, 684, 5656
PciSI GCTCTTC 2 cut(s) 3647, 5898
PctI GAATGC 5 cut(s) 1141, 2165, 3616, 4661, 5753
PdmI GAANNNNTTC 2 cut(s) 3071, 5245
PflMI CCANNNNNTGG 1 cut(s) 2457
PfoI TCCNGGA 2 cut(s) 1351, 5440
PkrI GCNGC 9 cut(s) 305, 1129, 2518, 2716, 2839, 3119, 3593, 4660, 5708
PleI GAGTC 8 cut(s) 499, 1317, 2254, 2453, 2838, 4502, 5391, 5876
PpsI GAGTC 8 cut(s) 499, 1317, 2254, 2453, 2838, 4502, 5391, 5876
Ppu21I YACGTR 2 cut(s) 444, 5540
PpuMI RGGWCCY 1 cut(s) 6107
PscI ACATGT 3 cut(s) 333, 684, 5656
PshAI GACNNNNGTC 1 cut(s) 4803
PshBI ATTAAT 5 cut(s) 1835, 1908, 2303, 2622, 3851
PsiI TTATAA 1 cut(s) 1584
Psp124BI GAGCTC 1 cut(s) 3642
Psp5II RGGWCCY 1 cut(s) 6107
Psp6I CCWGG 9 cut(s) 201, 809, 925, 1351, 1860, 3995, 5440, 5831, 6034
PspFI CCCAGC 3 cut(s) 462, 3788, 5061
PspGI CCWGG 9 cut(s) 201, 809, 925, 1351, 1860, 3995, 5440, 5831, 6034
PspOMI GGGCCC 1 cut(s) 1447
PspPPI RGGWCCY 1 cut(s) 6107
PsrI GAACNNNNNNTAC 2 cut(s) 693, 725
PstI CTGCAG 5 cut(s) 2560, 4455, 4468, 5155, 5382
PstNI CAGNNNCTG 1 cut(s) 3728
PsuI RGATCY 3 cut(s) 4044, 5437, 5512
PvuII CAGCTG 1 cut(s) 2717
RseI CAYNNNNRTG 8 cut(s) 620, 999, 3007, 3196, 4433, 5456, 5742, 6144
SacI GAGCTC 1 cut(s) 3642
SapI GCTCTTC 2 cut(s) 3647, 5898
SatI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2838, 3118, 3592, 4659, 5707
ScaI AGTACT 1 cut(s) 1024
SchI GAGTC 8 cut(s) 500, 1318, 2255, 2454, 2838, 4502, 5392, 5877
ScrFI CCNGG 9 cut(s) 203, 811, 927, 1353, 1862, 3997, 5442, 5833, 6036
SexAI ACCWGGT 1 cut(s) 6034
SfcI CTRYAG 8 cut(s) 1846, 2556, 4451, 4464, 4934, 5151, 5378, 5643
SinI GGWCC 6 cut(s) 71, 188, 2342, 2707, 5933, 6107
SmiMI CAYNNNNRTG 8 cut(s) 620, 999, 3007, 3196, 4433, 5456, 5742, 6144
SmlI CTYRAG 6 cut(s) 797, 869, 1409, 3355, 3427, 3920
SmoI CTYRAG 6 cut(s) 797, 869, 1409, 3355, 3427, 3920
SnaBI TACGTA 2 cut(s) 444, 5540
SpeI ACTAGT 1 cut(s) 1340
SphI GCATGC 3 cut(s) 3514, 4636, 5498
SseBI AGGCCT 1 cut(s) 3671
SspI AATATT 6 cut(s) 1588, 1803, 2774, 2901, 3028, 5069
SstI GAGCTC 1 cut(s) 3642
StuI AGGCCT 1 cut(s) 3671
StyD4I CCNGG 9 cut(s) 201, 809, 925, 1351, 1860, 3995, 5440, 5831, 6034
StyI CCWWGG 3 cut(s) 191, 718, 6123
TaiI ACGT 7 cut(s) 343, 446, 1292, 3550, 4481, 5542, 6085
TaqI TCGA 6 cut(s) 281, 2479, 3207, 3715, 4182, 5166
TaqII GACCGA 2 cut(s) 1492, 5678
TatI WGTACW 9 cut(s) 612, 1022, 1384, 1495, 2647, 3730, 5470, 5653, 6148
TauI GCSGC 1 cut(s) 3594
TseFI GTSAC 6 cut(s) 336, 1365, 2257, 2789, 3553, 4474
TseI GCWGC 8 cut(s) 303, 1127, 2516, 2714, 2837, 3117, 4658, 5706
Tsp45I GTSAC 6 cut(s) 336, 1365, 2257, 2789, 3553, 4474
TspGWI ACGGA 2 cut(s) 541, 2718
Van91I CCANNNNNTGG 1 cut(s) 2457
Vha464I CTTAAG 1 cut(s) 3355
VneI GTGCAC 2 cut(s) 564, 5197
VpaK11BI GGWCC 6 cut(s) 71, 188, 2342, 2707, 5933, 6107
VspI ATTAAT 5 cut(s) 1835, 1908, 2303, 2622, 3851
XbaI TCTAGA 2 cut(s) 1711, 2107
XcmI CCANNNNNNNNNTGG 1 cut(s) 3003
XmiI GTMKAC 2 cut(s) 440, 5542
XmnI GAANNNNTTC 2 cut(s) 3071, 5245
ZrmI AGTACT 1 cut(s) 1024
Zsp2I ATGCAT 5 cut(s) 1141, 1515, 2236, 3037, 4961
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.