Urb2/Npa2 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1216 |
| Acc36I | ACCTGC | 1 cut(s) | 1216 |
| AccBSI | CCGCTC | 3 cut(s) | 69, 94, 577 |
| AciI | CCGC | 4 cut(s) | 69, 92, 106, 575 |
| AclWI | GGATC | 2 cut(s) | 361, 374 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 1 cut(s) | 699 |
| AcuI | CTGAAG | 2 cut(s) | 450, 554 |
| AcyI | GRCGYC | 1 cut(s) | 621 |
| AfaI | GTAC | 4 cut(s) | 610, 817, 1086, 1177 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 182, 793 |
| AflIII | ACRYGT | 1 cut(s) | 333 |
| AgsI | TTSAA | 6 cut(s) | 155, 545, 846, 937, 1019, 1162 |
| AhlI | ACTAGT | 1 cut(s) | 929 |
| AjnI | CCWGG | 1 cut(s) | 201 |
| Alw26I | GTCTC | 2 cut(s) | 25, 485 |
| AlwI | GGATC | 2 cut(s) | 361, 374 |
| AoxI | GGCC | 2 cut(s) | 3, 89 |
| ApeKI | GCWGC | 2 cut(s) | 303, 707 |
| ApoI | RAATTY | 1 cut(s) | 699 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 134, 166 |
| AseI | ATTAAT | 2 cut(s) | 444, 1272 |
| AspS9I | GGNCC | 4 cut(s) | 71, 188, 1036, 1205 |
| AsuHPI | GGTGA | 4 cut(s) | 298, 563, 1240, 1343 |
| AvaII | GGWCC | 4 cut(s) | 71, 188, 1036, 1205 |
| BamHI | GGATCC | 1 cut(s) | 366 |
| BauI | CACGAG | 1 cut(s) | 473 |
| BbvI | GCAGC | 2 cut(s) | 315, 694 |
| BccI | CCATC | 4 cut(s) | 581, 820, 1042, 1283 |
| BceAI | ACGGC | 1 cut(s) | 1334 |
| BciT130I | CCWGG | 1 cut(s) | 203 |
| BclI | TGATCA | 1 cut(s) | 772 |
| BcoDI | GTCTC | 2 cut(s) | 25, 485 |
| BcuI | ACTAGT | 1 cut(s) | 929 |
| BfaI | CTAG | 3 cut(s) | 260, 930, 1308 |
| BfuAI | ACCTGC | 1 cut(s) | 1216 |
| BisI | GCNGC | 3 cut(s) | 92, 304, 708 |
| BlsI | GCNGC | 3 cut(s) | 93, 305, 709 |
| BmcAI | AGTACT | 1 cut(s) | 610 |
| Bme1390I | CCNGG | 1 cut(s) | 203 |
| Bme18I | GGWCC | 4 cut(s) | 71, 188, 1036, 1205 |
| BmgT120I | GGNCC | 4 cut(s) | 71, 188, 1036, 1205 |
| BmiI | GGNNCC | 5 cut(s) | 73, 200, 368, 987, 1038 |
| BmrFI | CCNGG | 1 cut(s) | 203 |
| BmsI | GCATC | 4 cut(s) | 430, 478, 496, 1120 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1118, 1150 |
| BpmI | CTGGAG | 3 cut(s) | 33, 224, 476 |
| BpuEI | CTTGAG | 2 cut(s) | 476, 1019 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1194 |
| BsaHI | GRCGYC | 1 cut(s) | 621 |
| BsaI | GGTCTC | 1 cut(s) | 485 |
| BsaJI | CCNNGG | 1 cut(s) | 191 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 182, 793 |
| Bse1I | ACTGG | 2 cut(s) | 370, 634 |
| Bse3DI | GCAATG | 2 cut(s) | 1057, 1220 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1194 |
| BseBI | CCWGG | 1 cut(s) | 203 |
| BseDI | CCNNGG | 1 cut(s) | 191 |
| BseGI | GGATG | 3 cut(s) | 626, 1057, 1135 |
| BseJI | GATNNNNATC | 1 cut(s) | 1194 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 182, 793 |
| BseMI | GCAATG | 2 cut(s) | 1057, 1220 |
| BseNI | ACTGG | 2 cut(s) | 370, 634 |
| BseXI | GCAGC | 2 cut(s) | 315, 694 |
| BshFI | GGCC | 2 cut(s) | 5, 91 |
| BslFI | GGGAC | 1 cut(s) | 1022 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 182, 793 |
| BsmAI | GTCTC | 2 cut(s) | 25, 485 |
| BsmFI | GGGAC | 1 cut(s) | 1022 |
| BsmI | GAATGC | 1 cut(s) | 721 |
| BsnI | GGCC | 2 cut(s) | 5, 91 |
| Bso31I | GGTCTC | 1 cut(s) | 485 |
| Bsp143I | GATC | 3 cut(s) | 238, 366, 772 |
| Bsp19I | CCATGG | 1 cut(s) | 191 |
| BspACI | CCGC | 4 cut(s) | 69, 92, 106, 575 |
| BspANI | GGCC | 2 cut(s) | 5, 91 |
| BspLI | GGNNCC | 5 cut(s) | 73, 200, 368, 987, 1038 |
| BspMI | ACCTGC | 1 cut(s) | 1216 |
| BspPI | GGATC | 2 cut(s) | 361, 374 |
| BspTNI | GGTCTC | 1 cut(s) | 485 |
| BsrBI | CCGCTC | 3 cut(s) | 69, 94, 577 |
| BsrDI | GCAATG | 2 cut(s) | 1057, 1220 |
| BsrI | ACTGG | 2 cut(s) | 370, 634 |
| BssECI | CCNNGG | 1 cut(s) | 191 |
| BssMI | GATC | 3 cut(s) | 238, 366, 772 |
| BssNI | GRCGYC | 1 cut(s) | 621 |
| BssSI | CACGAG | 1 cut(s) | 473 |
| BssT1I | CCWWGG | 1 cut(s) | 191 |
| Bst2BI | CACGAG | 1 cut(s) | 473 |
| Bst2UI | CCWGG | 1 cut(s) | 203 |
| Bst6I | CTCTTC | 1 cut(s) | 50 |
| BstACI | GRCGYC | 1 cut(s) | 621 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 716, 809 |
| BstDEI | CTNAG | 1 cut(s) | 1023 |
| BstDSI | CCRYGG | 1 cut(s) | 191 |
| BstF5I | GGATG | 3 cut(s) | 626, 1057, 1135 |
| BstKTI | GATC | 3 cut(s) | 241, 369, 775 |
| BstMAI | GTCTC | 2 cut(s) | 25, 485 |
| BstMBI | GATC | 3 cut(s) | 238, 366, 772 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 457, 466, 716, 809, 994 |
| BstNI | CCWGG | 1 cut(s) | 203 |
| BstNSI | RCATGY | 1 cut(s) | 337 |
| BstSCI | CCNGG | 1 cut(s) | 201 |
| BstV1I | GCAGC | 2 cut(s) | 315, 694 |
| BstX2I | RGATCY | 1 cut(s) | 366 |
| BstXI | CCANNNNNNTGG | 3 cut(s) | 500, 587, 826 |
| BstYI | RGATCY | 1 cut(s) | 366 |
| BsuRI | GGCC | 2 cut(s) | 5, 91 |
| BtgI | CCRYGG | 1 cut(s) | 191 |
| BtgZI | GCGATG | 1 cut(s) | 1302 |
| BtsCI | GGATG | 3 cut(s) | 626, 1057, 1135 |
| BtsI | GCAGTG | 2 cut(s) | 313, 333 |
| BtsIMutI | CAGTG | 4 cut(s) | 313, 333, 364, 803 |
| BveI | ACCTGC | 1 cut(s) | 1216 |
| Cfr13I | GGNCC | 4 cut(s) | 71, 188, 1036, 1205 |
| CseI | GACGC | 1 cut(s) | 629 |
| Csp6I | GTAC | 4 cut(s) | 609, 816, 1085, 1176 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 166, 201 |
| CviAII | CATG | 6 cut(s) | 192, 266, 334, 560, 823, 1103 |
| CviQI | GTAC | 4 cut(s) | 609, 816, 1085, 1176 |
| DdeI | CTNAG | 1 cut(s) | 1023 |
| DpnI | GATC | 3 cut(s) | 240, 368, 774 |
| DpnII | GATC | 3 cut(s) | 238, 366, 772 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| Eam1104I | CTCTTC | 1 cut(s) | 50 |
| EarI | CTCTTC | 1 cut(s) | 50 |
| Eco130I | CCWWGG | 1 cut(s) | 191 |
| Eco31I | GGTCTC | 1 cut(s) | 485 |
| Eco47I | GGWCC | 4 cut(s) | 71, 188, 1036, 1205 |
| Eco57I | CTGAAG | 2 cut(s) | 450, 554 |
| EcoRII | CCWGG | 1 cut(s) | 201 |
| EcoT14I | CCWWGG | 1 cut(s) | 191 |
| EcoT22I | ATGCAT | 2 cut(s) | 721, 1104 |
| ErhI | CCWWGG | 1 cut(s) | 191 |
| FaeI | CATG | 6 cut(s) | 195, 269, 337, 563, 826, 1106 |
| FaqI | GGGAC | 1 cut(s) | 1022 |
| FatI | CATG | 6 cut(s) | 191, 265, 333, 559, 822, 1102 |
| FauI | CCCGC | 1 cut(s) | 582 |
| FauNDI | CATATG | 1 cut(s) | 1339 |
| FbaI | TGATCA | 1 cut(s) | 772 |
| Fnu4HI | GCNGC | 3 cut(s) | 92, 304, 708 |
| FokI | GGATG | 3 cut(s) | 613, 1064, 1142 |
| Fsp4HI | GCNGC | 3 cut(s) | 92, 304, 708 |
| FspBI | CTAG | 3 cut(s) | 260, 930, 1308 |
| GluI | GCNGC | 3 cut(s) | 92, 304, 708 |
| GsuI | CTGGAG | 3 cut(s) | 33, 224, 476 |
| HaeIII | GGCC | 2 cut(s) | 5, 91 |
| HgaI | GACGC | 1 cut(s) | 629 |
| Hin1I | GRCGYC | 1 cut(s) | 621 |
| Hin1II | CATG | 6 cut(s) | 195, 269, 337, 563, 826, 1106 |
| HindIII | AAGCTT | 1 cut(s) | 536 |
| HinfI | GANTC | 5 cut(s) | 8, 80, 211, 644, 797 |
| HphI | GGTGA | 4 cut(s) | 298, 563, 1240, 1343 |
| Hpy166II | GTNNAC | 2 cut(s) | 331, 882 |
| Hpy188I | TCNGA | 4 cut(s) | 85, 121, 388, 895 |
| Hpy188III | TCNNGA | 8 cut(s) | 12, 223, 493, 512, 648, 941, 1016, 1159 |
| Hpy8I | GTNNAC | 2 cut(s) | 331, 882 |
| HpyAV | CCTTC | 5 cut(s) | 244, 283, 290, 383, 833 |
| HpyCH4IV | ACGT | 1 cut(s) | 340 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 457, 466, 716, 809, 994 |
| HpyF3I | CTNAG | 1 cut(s) | 1023 |
| HpySE526I | ACGT | 1 cut(s) | 340 |
| Hsp92I | GRCGYC | 1 cut(s) | 621 |
| Hsp92II | CATG | 6 cut(s) | 195, 269, 337, 563, 826, 1106 |
| Ksp22I | TGATCA | 1 cut(s) | 772 |
| Kzo9I | GATC | 3 cut(s) | 238, 366, 772 |
| LmnI | GCTCC | 6 cut(s) | 14, 456, 582, 597, 985, 1142 |
| Lsp1109I | GCAGC | 2 cut(s) | 315, 694 |
| LweI | GCATC | 4 cut(s) | 430, 478, 496, 1120 |
| MaeI | CTAG | 3 cut(s) | 260, 930, 1308 |
| MaeII | ACGT | 1 cut(s) | 340 |
| MaeIII | GTNAC | 1 cut(s) | 336 |
| MalI | GATC | 3 cut(s) | 240, 368, 774 |
| MbiI | CCGCTC | 3 cut(s) | 69, 94, 577 |
| MboI | GATC | 3 cut(s) | 238, 366, 772 |
| MboII | GAAGA | 9 cut(s) | 55, 64, 67, 89, 233, 305, 557, 869, 1174 |
| MflI | RGATCY | 1 cut(s) | 366 |
| MluCI | AATT | 5 cut(s) | 348, 699, 1043, 1153, 1273 |
| MmeI | TCCRAC | 3 cut(s) | 139, 654, 889 |
| Mph1103I | ATGCAT | 2 cut(s) | 721, 1104 |
| MseI | TTAA | 5 cut(s) | 444, 780, 1068, 1152, 1272 |
| MslI | CAYNNNNRTG | 1 cut(s) | 585 |
| MspR9I | CCNGG | 1 cut(s) | 203 |
| Mva1269I | GAATGC | 1 cut(s) | 721 |
| MvaI | CCWGG | 1 cut(s) | 203 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 457, 466, 716, 809, 994 |
| NcoI | CCATGG | 1 cut(s) | 191 |
| NdeI | CATATG | 1 cut(s) | 1339 |
| NdeII | GATC | 3 cut(s) | 238, 366, 772 |
| NlaIII | CATG | 6 cut(s) | 195, 269, 337, 563, 826, 1106 |
| NlaIV | GGNNCC | 5 cut(s) | 73, 200, 368, 987, 1038 |
| NmuCI | GTSAC | 1 cut(s) | 336 |
| NsiI | ATGCAT | 2 cut(s) | 721, 1104 |
| NspI | RCATGY | 1 cut(s) | 337 |
| PaqCI | CACCTGC | 1 cut(s) | 1216 |
| PciI | ACATGT | 1 cut(s) | 333 |
| PctI | GAATGC | 1 cut(s) | 721 |
| PfeI | GAWTC | 5 cut(s) | 8, 80, 211, 644, 797 |
| PkrI | GCNGC | 3 cut(s) | 93, 305, 709 |
| PscI | ACATGT | 1 cut(s) | 333 |
| PshBI | ATTAAT | 2 cut(s) | 444, 1272 |
| Psp6I | CCWGG | 1 cut(s) | 201 |
| PspGI | CCWGG | 1 cut(s) | 201 |
| PspN4I | GGNNCC | 5 cut(s) | 73, 200, 368, 987, 1038 |
| PspPI | GGNCC | 4 cut(s) | 71, 188, 1036, 1205 |
| PsuI | RGATCY | 1 cut(s) | 366 |
| RsaI | GTAC | 4 cut(s) | 610, 817, 1086, 1177 |
| RsaNI | GTAC | 4 cut(s) | 609, 816, 1085, 1176 |
| RseI | CAYNNNNRTG | 1 cut(s) | 585 |
| SaqAI | TTAA | 5 cut(s) | 444, 780, 1068, 1152, 1272 |
| SatI | GCNGC | 3 cut(s) | 92, 304, 708 |
| Sau3AI | GATC | 3 cut(s) | 238, 366, 772 |
| Sau96I | GGNCC | 4 cut(s) | 71, 188, 1036, 1205 |
| ScaI | AGTACT | 1 cut(s) | 610 |
| ScrFI | CCNGG | 1 cut(s) | 203 |
| SfaNI | GCATC | 4 cut(s) | 430, 478, 496, 1120 |
| SinI | GGWCC | 4 cut(s) | 71, 188, 1036, 1205 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 585 |
| SmlI | CTYRAG | 2 cut(s) | 455, 998 |
| SmoI | CTYRAG | 2 cut(s) | 455, 998 |
| SpeI | ACTAGT | 1 cut(s) | 929 |
| Sse9I | AATT | 5 cut(s) | 348, 699, 1043, 1153, 1273 |
| SsiI | CCGC | 4 cut(s) | 69, 92, 106, 575 |
| SspMI | CTAG | 3 cut(s) | 260, 930, 1308 |
| StyD4I | CCNGG | 1 cut(s) | 201 |
| StyI | CCWWGG | 1 cut(s) | 191 |
| TaiI | ACGT | 1 cut(s) | 343 |
| TaqI | TCGA | 1 cut(s) | 281 |
| TaqII | GACCGA | 2 cut(s) | 860, 1081 |
| TasI | AATT | 5 cut(s) | 348, 699, 1043, 1153, 1273 |
| TatI | WGTACW | 3 cut(s) | 608, 1084, 1175 |
| TauI | GCSGC | 1 cut(s) | 94 |
| TfiI | GAWTC | 5 cut(s) | 8, 80, 211, 644, 797 |
| Tru1I | TTAA | 5 cut(s) | 444, 780, 1068, 1152, 1272 |
| Tru9I | TTAA | 5 cut(s) | 444, 780, 1068, 1152, 1272 |
| TscAI | CASTG | 4 cut(s) | 313, 333, 364, 810 |
| TseFI | GTSAC | 1 cut(s) | 336 |
| TseI | GCWGC | 2 cut(s) | 303, 707 |
| Tsp45I | GTSAC | 1 cut(s) | 336 |
| TspDTI | ATGAA | 4 cut(s) | 147, 855, 1046, 1119 |
| TspRI | CASTG | 4 cut(s) | 313, 333, 364, 810 |
| VpaK11BI | GGWCC | 4 cut(s) | 71, 188, 1036, 1205 |
| VspI | ATTAAT | 2 cut(s) | 444, 1272 |
| XapI | RAATTY | 1 cut(s) | 699 |
| XceI | RCATGY | 1 cut(s) | 337 |
| XspI | CTAG | 3 cut(s) | 260, 930, 1308 |
| ZrmI | AGTACT | 1 cut(s) | 610 |
| Zsp2I | ATGCAT | 2 cut(s) | 721, 1104 |