Rroxscaffold_7G00215270

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000376
Physical Location & Seq
Forward (+)
65890917 .. 65901696
10780 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215270.1

Sequence Viewer

Length: 6210 bp
ATGGCCGATTCTGGAGCTAACCCTAAACAGAGACAACCCAAGAAGAACAAGAAGAAGAGAAAGCAGAGCGGACCCCAAGAATCTGAAAGGCCACCCAAAACTAACCGCACCAATTCATCAGAGAACGAGAATGAACCACAAGACCAAAACTTTGAAGTTGGAAAAGCTGAAGAACCCAATCAAGGTGGTCCATGGAGGAACCTGGAGTTGATTCTGTCAATCCAGAACAGAGAGCTTGATCTTCACAAGAAGGTGGAACTAGCTCATGGTTTTGTGATGTCAAGGGTGAAGGAAGAAGGAGGCAGCAGTGATGAGGATAATCAAGCAGTGAACATGTCACGTTTAGTAATTTTTGTCAGTGATTGGATCCAGTCATTGTTGATTTCCGAAGGAAAGAAAGTTGAGAGTGGTGGAGAGAAGCATCAAGCTGAAGTTATTTATACGTATTTGGATTTAAGATGCTGGGAGATTTTCAAATTCTGTTTGGAGGAGTCTCTGAAATTAAATGTTTCGTTAAGTTTCTCACGGAACCTTTTACGCTCTATATGTTGGATTGCAAGGAGCGCACTGTCCTTATTGAACAAAAAAATTATTGGTGAAGGGTTACAATTGTACACTACTGTGCTTGATTGTATTTCTTTGGTATTTTCATCCCAAGAAGGCTTCTCCAATGAAAATTTAGACATGTGGATTATAACTGTAAGTTCAGTTCTTGACCTTGGACACAGATTTTACAGTGAAGCTCTTGTTAGTGCCAATGAGGGTGTATTTGTTTTCCAATTCTTGTGCTTGGTGCTTGAGCCATTTGCCAGGTTTTTAAGGGCTCACGCTCGGAAAGATGGATTTCGTGATTTTAGTAATAAGCTCCTTGAGCCTCTGTTGCATCTCTTGGGTCTCTTGCATCTCCAGATTGGTGGAAGTAATCCTGGTTGGAGAAGAAGCTTATTGAAGATTGTTGAAGATGTATTATCTCATGGGCTATATCACCCGCTCCATATTGATGGGTTTTTGAGCTTATGCAGTACTGAAAAAGACGCCACATCCAATGATGGGAAGTCAAAAGATTCCAAAACAATCAACCAGAGTTATCATAGACATTTATTTGATAAAGTGGAAGGAATTTTAGCTGCAAAGAATGCATTTGCAGTAGAAAGCATAGGAGTATTGTTTCACTTGCTTATTGATCAAGTTAAAAAGCTAAAGAGGGATTCAGCGCTGACTGCAAGTACCAAAATGATGGGGAAGAGTGAAGGTTCAAGGCAGTTAGAAGATAGTTCATTGGGTCAAACGTCTATGATGTCTTTTGAGAGTCGTATTGGTGAAAAGAATTATTGCTCAACTAGTTTCAATCCAGGAACACGAAAGTCACTTCACGATTTTTTTGTACTGATTATGGAGCCTCTTGTGCTTGAGATCAATGGCTTTCTTGAAACTAAGCTGAAAGTGGGCCCTGAATTGTTGGATATTCATTGCACACTTAAAGCTGTAAATAATGTTCTTTCGGTCTTTATGCATGAAAAGATTTATGTGAGAACAGAGGATGCCTCTGAGGGAGCTTCTCTTAATTTCTTGAAGAAGGTTTATAATATTATCATCTCATTATCTTCAGATTTAATTCAGTTGTCAGTTACACACTCAGATAGTTTTACTCTAATAGCAAGTGAGGTACTTTCTGCTGTTGGCTATCTTTTGGAAATTGAGTATGATGTTCTGGAGAATGATCTAGTAAGCTTGTGGTTAATGATCCTCTCTTACTGGGCCATTGGGCTTTCTTTGGTGGATTCTCCGGATAGATGCTCATTATTCTCCAAGATAACAGATATTGGATGTCAATTAATTATGCTCTATAGTCAACTTCGCCAGGTGAATAATACTATCTTTGCATTGTGTAAAGCAATAAGGGTTATTAATTTACATAATAGCGATGGTGAACTGAACTATACTAGATTTGTCATTCCTTTTCATGGTGAAGAATATGCAAAAGCTGTGGAAATGCTATTATGTGCACATGAATTCAAAATTGCAATCCATAAAGCAATGAAGTCCATACCAGAAGGACAAGCAAGTCGGTGTATTTGGCAGTTAACATTGGATATATCAGAATCTGTAGAGTGGATGAAAGTTAGTTGTTCGGAGGGTGATGAAAAAGAATTTTCTGAATGCCATCTAAGTAGCTTGCACAGTTTTAATCTGGAAGCTGAACTTTTCGGGAGAGGGTTGTCAGAAATATATGCATTATTCCTAGAGTCATTGATTGTCACAACAGGAAACTGTAACCTTCTCGGCGCTTCTATAAAGGAATTAATTAGAGTGATTTTCCCTTGCATGAGCAATCTGGTTGAACCACAGCAGGAGGATGCTGTAAACAAATTCCTATGGTCTGTCGCGGTAAAAGATTCTGACAATGTGATCGCTAGAAACAAAAACAAATATCTGATATTTGGAGTCTCCACTTATTGGGTAGTTTTGTTCTTCTTTCGATTGTATAGCTCCAGCCGATGCTTATATAGGCAAGCAGCAACCCTTATGCCTCCAGATTTATCAAGAAAGATGTCTGCAGAAATGGGAGATTCGTTCTCCTCATTCTCTGGCAGGGATTGGATGGAAATGTCTGATTGGATCAATGGGGGATTTTTCTCTTGGATTGTACAACCTTCAGCTTCTCTTCTTTCTGTTATTCAATCTGTTTCAAACATCTTTTGTAAGGACCGTGCAGCTGATTCTTGTCCTTTAACTTATGTAATGCATGTTATGGCTTGTGAAAGGCTTGTGGATTTGAATAGTCGTTTAAAGTCATTTGAATATTTGATTGAAAATGGTGACAATCTGGTGCAAATTGCGGAGATCTCTTCTTTGAGGCAGGAGGCAGCAGGACTCACAGGATTCATGATGGAGCATCTCTCATTAGTGTCTGAAGATCAACAGCGAATATTCATTTCTGCTGATACGACTAATAATAAGATGGTTAACTATGAAAGTGATGAATGGGATTTCAGTATTTGCTCTGTGAACAAGAAGTCATTGCCAACTGCACTTTGGTGGGTTGTTTGCCAAAATATTCATGCATGGTGCCCTCATGCCTCTAAAAAGGATTTGAAGAGATTCCTTTCTCTTTTAATCCACACTTCAATTCCCTATGTAAGAAGCAGCTTTGGGGTGGTTATAGAGCACAAGAACCATGAAGCTGACAGGCTGAAGAATGTGGCATTGCATCAAATCTCATCACAGTGCTTTATTGATTCCAGTCTGTATGAGCAAAGATTTGTCCGCAGGTATTTTGCAAAGAGGTTCTGCCGTGCATTGGAGAAATCTACATTACCATTCATCCGCGACTTTCCATCCGGCAATGTTAAATTCAAATCATCACCTAATTGGCCAAATGTCTTAAGTGATTTAGAGAACTCATCATTGGCTATTTCCTGCAACAAACTCAATGTATTTGATTGTTCCTCAGCCTCAAGTTGCAAGGGGCAAAACTCCCAACCATCTACCATCAGGAAATTTACAGCTTGTCAGAGTTTGCTTAATCTTTTGAGTTGTATGCCAAAAGGTCATTTGGACACGAGATCATTTTCACATTATGTGACTTCTATTCTGAACCTCGAAAGGATTGTTGTTGGCGGCTTATTAGATTATCAGAATGCTTCATACTCAACTTATTATTATGAGCTCTTCAGATTGTTTGTGTCCTGTCGGAAGGCCTTAAGATTTATAATTACTGCTTGTGAGGAGACAGTAGCCAGTCGAACCTCAGACACCGTACTCTTTGAGGATTTATTTCCAGTTTCATGGCTTTACAAGTCAGTGCATATGGTTGCTGGGCTTCAAGAATCATTCTCAAAAGATATTTATCATCAAGTCCATGATATGATCTTGGCGTTAATGGATCACACATTTTATGTGTTTCTGACATTAAATAAATATCAATCCAATCATGCTGTTCGTTTCCTTGAGGTTGCTGAGCTGAATTTTGGATGTGCCCAAGAACAGAGAAGTTTATTGAATTCGTCCAATTATATTGAAGCCTGGAAAAGTGTAAACGTTGTTGCTAAGATTTTGAAGGAACAGATGCAGATCTTACTTCTAAATGTGAAGAATGGCATTTGTAATGGAAAAGAAGAAGTTTCTGTTGATGCTTTAAATTTGAACAAGTTTGCTTCCATAATTTCGTGTTTTAGTGGGTTTTTATGGGGCCTAGCTTGCGTTGTGATCGACACAGATGGGAGAAATAGTGATGAGAAAGCTAAGTTGTCAAGGTGGAAACTTGAACCAATCTCCGAACTCAACCTCTGTATAAAGGTTTTTGAAGAAATTTCTAGTCTTCTACTACAAATGTTTATTCTTGATGATAATCAGCAGTCCACAGCTATATGTCATGCCTATAATCTTCAGAAGTCTGGCTACAATTTAGATTTGTTTGGTGCAGAGAAGATTTTGCCAGAAGGCATTGATACTGACACTGATATGGCTTGTGGTGGACTGCAGGATGAATCTGCAGTTGCAGTGACGTGCTCAGCATCATCTGACATCTGTGAAGACTCTGTAATTGGTAGTGTTCATAGAAGAAGACCACGCTTGAAAGATGCAAATAGTGTTGTTAGTGTTCTCACTGCTGTTGATTCATTTGAACTGCAATTTTTAAATAAGCCTTTGTTGAGAAGCATGCTAAAAGGTGATTTCCCCAACGCAGCATTTTTACTCAGGCAGTTGTTAATCGCCTCTTCTGCTATTTTGAGGCTAAATTTGCATATTAAGAGCGCTCCCATGTTGTCAAGCTTGGTGCATAAGTTTGCTGGCATTATGCAAGTCTTGTTATTGGAATCAGCGGACCCTATTCAGGTGCCACACTTCTATTATTTTGTGTGTTTAGATGGTGTTCTGAAGTATCTGGAAGAATTAGGGAATCATTTTCCTTTGACTAATCCTGCCTTATCCAGAGATTTGTTTGACAAGATGGTTCAGCTACAGTTACGGGCCCTAGGGAAATGCATAACTTTACAAGGGAAAAGAGCAACTCTTGCATCTCATGAAACAAGCACCAACACACCCATAGGAGAAAGGAGATTTTCTGAAGCATCATCTTTTTCTGGCTGGGAATATTTATTGGATGAATTCAAAGCTAGGTTGAGGTCATCATTCGCCGTGTTCATAAAGAAATCAACAGAGTTGCATCTCCAATCTGCAGTTCAAGCTATTGAAAGAGCCCTAGTTGGTGTGCAGGAAGGGTGCACCGTGAGGTATGACATATGTGCTGGAAATGAAGATGGAGGAAAGGTTTCTTCAATTGTTGCATCTGGCATTGATTGCTTGGATTTGGTTCTGGAATTTGTTTCAGGACGCAATTTGATTGTGGTTAAAAAATACATTCAGAGGTTAATTGCCTGCATGTTCAATGTTATTCTGCATCTGCAGAGTCCACTAATCTTCTATGAGAGATTCACTCAGAGTAAAGATGATAGTGATCCAGATCCAGGAACAGTCATTCTTATGTGTGTTGACGTACTGGCAAGAATTTCTGGCAAGCATGCAATATACAAAATGGATCTCTGGCATGTAGCACACTCGTTACGTATACCTTCAGCACTTTTTCAAGATTTCCATCTGTTAAAACATTCTAAATGTCCTGTTCCAAATGATTCTTCAACAAGTCCAAATAACCAACTCTCCGACCCAGTAGCAAGTGCACATGTCTCTGGTATAGATAGGCAATACTCAATAGGCCTATATTCTGCTTGCTGCCGATTATTGCATAATGTTGTGAAGCATCACAAAAGTGAATGCGAGGGGTATGTTGCTCTACTTCAAGCTTCTGTTCGTGTTCTTCTTTATTGTTTGGAGACATTGGATGCTGTTGTGGTAGCCAGGGAAGGCTTCTTTTCATGGGAAGTAGAAGAAGGAGTCAAATGTGCATGTTCTCTCAGAAGGATTTATGAAGAGCTAAGACATCAAAAAGAGGTCTTTGGTCCACACTGTTACCACTTTTTAGCTTATTACATACGGGTTTATTCCGGATATGGCCCCCGCAAAACAGGCATCAAAAGGGAAATAGATGAAGCTCTAAGACCAGGTGTATATGCGTTGATAGATGTTTGCTCACCTGATGATCTTCAACGTCTTCATACCTCATTTGGAGAGGGTCCTTGCAGAAACACTCTGGCAACCTTGAAGCACGATTACGAACTCAATTTCCAGTACCAGGGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

2069

Amino Acids

233.42

Weight (kDa)

6.3

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1830 - 2068 4.1e-38 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 695, 1584, 3713
AasI GACNNNNNNGTC 1 cut(s) 2064
Acc36I ACCTGC 1 cut(s) 3261
AccB1I GGYRCC 2 cut(s) 3069, 4840
AccB7I CCANNNNNTGG 1 cut(s) 2457
AccBSI CCGCTC 2 cut(s) 69, 991
AccI GTMKAC 1 cut(s) 5572
AccII CGCG 2 cut(s) 2387, 3330
AccIII TCCGGA 2 cut(s) 1786, 6008
AclI AACGTT 1 cut(s) 4041
AclWI GGATC 8 cut(s) 361, 374, 1738, 2627, 3894, 5456, 5462, 5550
AcoI YGGCCR 2 cut(s) 3, 3374
AcyI GRCGYC 1 cut(s) 1035
AfaI GTAC 9 cut(s) 614, 1024, 1228, 1386, 1668, 2649, 3762, 5502, 6194
AfeI AGCGCT 2 cut(s) 1215, 4759
AfiI CCNNNNNNNGG 8 cut(s) 182, 1050, 1964, 2457, 3301, 4837, 5471, 6196
AflII CTTAAG 2 cut(s) 3385, 3703
AflIII ACRYGT 3 cut(s) 333, 684, 5686
AhlI ACTAGT 1 cut(s) 1340
AjiI CACGTC 1 cut(s) 4509
AjuI GAANNNNNNNTTGG 6 cut(s) 3392, 3424, 5169, 5201, 5623, 5655
AleI CACNNNNGTG 3 cut(s) 620, 3037, 5772
AloI GAACNNNNNNTCC 2 cut(s) 6171, 6203
Alw21I GWGCWC 6 cut(s) 2008, 3171, 3672, 4514, 5231, 5686
Alw26I GTCTC 7 cut(s) 25, 498, 899, 2452, 3725, 5695, 5831
Alw44I GTGCAC 3 cut(s) 2004, 5227, 5682
AlwI GGATC 8 cut(s) 361, 374, 1738, 2627, 3894, 5456, 5462, 5550
AlwNI CAGNNNCTG 1 cut(s) 2105
Aor13HI TCCGGA 2 cut(s) 1786, 6008
Aor51HI AGCGCT 2 cut(s) 1215, 4759
ApaI GGGCCC 2 cut(s) 1451, 4978
ApaLI GTGCAC 3 cut(s) 2004, 5227, 5682
ApeKI GCWGC 8 cut(s) 303, 1127, 2516, 2714, 2867, 3147, 4688, 5736
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AseI ATTAAT 3 cut(s) 1835, 1908, 2303
Asp700I GAANNNNTTC 2 cut(s) 3101, 5275
AspA2I CCTAGG 1 cut(s) 4978
AspLEI GCGC 4 cut(s) 566, 1216, 2288, 4760
AvaII GGWCC 6 cut(s) 71, 188, 2707, 4828, 5963, 6137
AvrII CCTAGG 1 cut(s) 4978
BaeGI GKGCMC 7 cut(s) 1451, 2008, 3074, 3982, 4978, 5231, 5686
BalI TGGCCA 1 cut(s) 3376
BamHI GGATCC 1 cut(s) 366
BanI GGYRCC 2 cut(s) 3069, 4840
BanII GRGCYC 5 cut(s) 826, 1451, 3672, 4978, 5206
BarI GAAGNNNNNNTAC 4 cut(s) 4385, 4417, 6158, 6190
BauI CACGAG 1 cut(s) 3562
BbsI GAAGAC 4 cut(s) 4313, 4542, 4573, 6107
Bbv12I GWGCWC 6 cut(s) 2008, 3171, 3672, 4514, 5231, 5686
BbvCI CCTCAGC 1 cut(s) 3451
BbvI GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2879, 3159, 4700, 5723
BceAI ACGGC 2 cut(s) 3279, 5126
BclI TGATCA 1 cut(s) 1183
BcoDI GTCTC 7 cut(s) 25, 498, 899, 2452, 3725, 5695, 5831
BcuI ACTAGT 1 cut(s) 1340
BfmI CTRYAG 8 cut(s) 1846, 2106, 2556, 4481, 4494, 4964, 5181, 5408
BfoI RGCGCY 3 cut(s) 1217, 2289, 4761
BfrI CTTAAG 2 cut(s) 3385, 3703
BfuAI ACCTGC 1 cut(s) 3261
BglII AGATCT 2 cut(s) 2844, 4074
BisI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2868, 3148, 3622, 4689, 5737
BlnI CCTAGG 1 cut(s) 4978
BlpI GCTNAGC 2 cut(s) 3960, 4513
BlsI GCNGC 9 cut(s) 305, 1129, 2518, 2716, 2869, 3149, 3623, 4690, 5738
BmcAI AGTACT 1 cut(s) 1024
Bme18I GGWCC 6 cut(s) 71, 188, 2707, 4828, 5963, 6137
BmgBI CACGTC 1 cut(s) 4509
BmrI ACTGGG 2 cut(s) 1765, 5666
BmuI ACTGGG 2 cut(s) 1765, 5666
BpiI GAAGAC 4 cut(s) 4313, 4542, 4573, 6107
BplI GAGNNNNNCTC 6 cut(s) 1529, 1561, 2885, 2917, 5425, 5457
BpmI CTGGAG 6 cut(s) 33, 224, 890, 1733, 2476, 2517
Bpu10I CCTNAGC 1 cut(s) 3451
Bpu1102I GCTNAGC 2 cut(s) 3960, 4513
BpuEI CTTGAG 5 cut(s) 818, 890, 1430, 3442, 3971
BsaAI YACGTR 2 cut(s) 444, 5570
BsaBI GATNNNNATC 6 cut(s) 3849, 4073, 4350, 5460, 5466, 5598
BsaHI GRCGYC 1 cut(s) 1035
BsaI GGTCTC 1 cut(s) 899
BsaJI CCNNGG 5 cut(s) 191, 718, 4978, 5862, 6196
BsaWI WCCGGW 2 cut(s) 1786, 6008
BsaXI ACNNNNNCTCC 6 cut(s) 4216, 4246, 5259, 5289, 5648, 5678
Bsc4I CCNNNNNNNGG 8 cut(s) 182, 1050, 1964, 2457, 3301, 4837, 5471, 6196
Bse1I ACTGG 8 cut(s) 370, 1760, 3243, 3741, 3782, 5508, 5672, 6190
Bse3DI GCAATG 5 cut(s) 1468, 2043, 3020, 3206, 3352
Bse8I GATNNNNATC 6 cut(s) 3849, 4073, 4350, 5460, 5466, 5598
BseAI TCCGGA 2 cut(s) 1786, 6008
BseDI CCNNGG 5 cut(s) 191, 718, 4978, 5862, 6196
BseJI GATNNNNATC 6 cut(s) 3849, 4073, 4350, 5460, 5466, 5598
BseLI CCNNNNNNNGG 8 cut(s) 182, 1050, 1964, 2457, 3301, 4837, 5471, 6196
BseMI GCAATG 5 cut(s) 1468, 2043, 3020, 3206, 3352
BseMII CTCAG 9 cut(s) 1539, 1650, 3465, 3765, 3951, 4527, 4714, 5456, 5932
BseNI ACTGG 8 cut(s) 370, 1760, 3243, 3741, 3782, 5508, 5672, 6190
BseRI GAGGAG 3 cut(s) 503, 2569, 3743
BseSI GKGCMC 7 cut(s) 1451, 2008, 3074, 3982, 4978, 5231, 5686
BseXI GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2879, 3159, 4700, 5723
BseYI CCCAGC 3 cut(s) 462, 3818, 5091
BsgI GTGCAG 4 cut(s) 2733, 3015, 4443, 5237
Bsh1236I CGCG 2 cut(s) 2387, 3330
BshNI GGYRCC 2 cut(s) 3069, 4840
BsiHKAI GWGCWC 6 cut(s) 2008, 3171, 3672, 4514, 5231, 5686
BsiSI CCGG 3 cut(s) 1787, 3342, 6009
BslI CCNNNNNNNGG 8 cut(s) 182, 1050, 1964, 2457, 3301, 4837, 5471, 6196
BsmAI GTCTC 7 cut(s) 25, 498, 899, 2452, 3725, 5695, 5831
BsmI GAATGC 4 cut(s) 1141, 2165, 3646, 5783
Bso31I GGTCTC 1 cut(s) 899
Bsp120I GGGCCC 2 cut(s) 1447, 4974
Bsp13I TCCGGA 2 cut(s) 1786, 6008
Bsp1407I TGTACA 2 cut(s) 612, 2647
Bsp1720I GCTNAGC 2 cut(s) 3960, 4513
Bsp19I CCATGG 1 cut(s) 191
BspCNI CTCAG 9 cut(s) 1540, 1649, 3464, 3764, 3952, 4526, 4713, 5455, 5931
BspEI TCCGGA 2 cut(s) 1786, 6008
BspFNI CGCG 2 cut(s) 2387, 3330
BspHI TCATGA 2 cut(s) 2886, 5026
BspMAI CTGCAG 5 cut(s) 2560, 4485, 4498, 5185, 5412
BspMI ACCTGC 1 cut(s) 3261
BspPI GGATC 8 cut(s) 361, 374, 1738, 2627, 3894, 5456, 5462, 5550
BspQI GCTCTTC 2 cut(s) 3677, 5928
BspT107I GGYRCC 2 cut(s) 3069, 4840
BspTI CTTAAG 2 cut(s) 3385, 3703
BspTNI GGTCTC 1 cut(s) 899
BsrBI CCGCTC 2 cut(s) 69, 991
BsrDI GCAATG 5 cut(s) 1468, 2043, 3020, 3206, 3352
BsrGI TGTACA 2 cut(s) 612, 2647
BsrI ACTGG 8 cut(s) 370, 1760, 3243, 3741, 3782, 5508, 5672, 6190
BssECI CCNNGG 5 cut(s) 191, 718, 4978, 5862, 6196
BssNAI GTATAC 1 cut(s) 5573
BssNI GRCGYC 1 cut(s) 1035
BssSI CACGAG 1 cut(s) 3562
BssT1I CCWWGG 3 cut(s) 191, 718, 4978
Bst1107I GTATAC 1 cut(s) 5573
Bst2BI CACGAG 1 cut(s) 3562
Bst6I CTCTTC 8 cut(s) 50, 1238, 2670, 2854, 3092, 3677, 4726, 5928
BstACI GRCGYC 1 cut(s) 1035
BstAFI CTTAAG 2 cut(s) 3385, 3703
BstAPI GCANNNNNTGC 3 cut(s) 1136, 5018, 5304
BstAUI TGTACA 2 cut(s) 612, 2647
BstBAI YACGTR 2 cut(s) 444, 5570
BstC8I GCNNGC 9 cut(s) 2177, 2514, 4201, 4664, 4795, 5383, 5522, 5526, 5734
BstDSI CCRYGG 1 cut(s) 191
BstFNI CGCG 2 cut(s) 2387, 3330
BstH2I RGCGCY 3 cut(s) 1217, 2289, 4761
BstHHI GCGC 4 cut(s) 566, 1216, 2288, 4760
BstMAI GTCTC 7 cut(s) 25, 498, 899, 2452, 3725, 5695, 5831
BstNSI RCATGY 9 cut(s) 337, 688, 2750, 4666, 5389, 5528, 5555, 5690, 5913
BstSFI CTRYAG 8 cut(s) 1846, 2106, 2556, 4481, 4494, 4964, 5181, 5408
BstSLI GKGCMC 7 cut(s) 1451, 2008, 3074, 3982, 4978, 5231, 5686
BstSNI TACGTA 2 cut(s) 444, 5570
BstUI CGCG 2 cut(s) 2387, 3330
BstV1I GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2879, 3159, 4700, 5723
BstV2I GAAGAC 4 cut(s) 4313, 4542, 4573, 6107
BstX2I RGATCY 5 cut(s) 366, 2844, 4074, 5467, 5542
BstXI CCANNNNNNTGG 4 cut(s) 914, 1001, 1237, 3789
BstYI RGATCY 5 cut(s) 366, 2844, 4074, 5467, 5542
BstZ17I GTATAC 1 cut(s) 5573
BtgI CCRYGG 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 1938
BtrI CACGTC 1 cut(s) 4509
BtsI GCAGTG 4 cut(s) 313, 333, 4509, 4608
BveI ACCTGC 1 cut(s) 3261
Cac8I GCNNGC 9 cut(s) 2177, 2514, 4201, 4664, 4795, 5383, 5522, 5526, 5734
CaiI CAGNNNCTG 1 cut(s) 2105
CciI TCATGA 2 cut(s) 2886, 5026
CfoI GCGC 4 cut(s) 566, 1216, 2288, 4760
CseI GACGC 2 cut(s) 1043, 5346
CsiI ACCWGGT 1 cut(s) 6064
Csp6I GTAC 9 cut(s) 613, 1023, 1227, 1385, 1667, 2648, 3761, 5501, 6193
CspCI CAANNNNNGTGG 4 cut(s) 166, 201, 1968, 2003
CviQI GTAC 9 cut(s) 613, 1023, 1227, 1385, 1667, 2648, 3761, 5501, 6193
DraI TTTAAA 3 cut(s) 2790, 4140, 4641
DrdI GACNNNNNNGTC 1 cut(s) 2064
DseDI GACNNNNNNGTC 1 cut(s) 2064
EaeI YGGCCR 2 cut(s) 3, 3374
Eam1104I CTCTTC 8 cut(s) 50, 1238, 2670, 2854, 3092, 3677, 4726, 5928
EarI CTCTTC 8 cut(s) 50, 1238, 2670, 2854, 3092, 3677, 4726, 5928
Ecl136II GAGCTC 1 cut(s) 3670
Eco105I TACGTA 2 cut(s) 444, 5570
Eco130I CCWWGG 3 cut(s) 191, 718, 4978
Eco147I AGGCCT 2 cut(s) 3701, 5721
Eco24I GRGCYC 5 cut(s) 826, 1451, 3672, 4978, 5206
Eco31I GGTCTC 1 cut(s) 899
Eco47I GGWCC 6 cut(s) 71, 188, 2707, 4828, 5963, 6137
Eco47III AGCGCT 2 cut(s) 1215, 4759
Eco53kI GAGCTC 1 cut(s) 3670
EcoICRI GAGCTC 1 cut(s) 3670
EcoO109I RGGNCCY 4 cut(s) 1448, 4192, 4975, 6137
EcoRI GAATTC 3 cut(s) 2012, 4003, 5111
EcoT14I CCWWGG 3 cut(s) 191, 718, 4978
EcoT22I ATGCAT 6 cut(s) 1141, 1515, 2236, 2748, 3067, 4991
EcoT38I GRGCYC 5 cut(s) 826, 1451, 3672, 4978, 5206
ErhI CCWWGG 3 cut(s) 191, 718, 4978
FalI AAGNNNNNCTT 2 cut(s) 2187, 2219
FauI CCCGC 2 cut(s) 996, 6029
FauNDI CATATG 2 cut(s) 3810, 5246
FbaI TGATCA 1 cut(s) 1183
FblI GTMKAC 1 cut(s) 5572
Fnu4HI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2868, 3148, 3622, 4689, 5737
FriOI GRGCYC 5 cut(s) 826, 1451, 3672, 4978, 5206
Fsp4HI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2868, 3148, 3622, 4689, 5737
GlaI GCGC 4 cut(s) 565, 1215, 2287, 4759
GluI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2868, 3148, 3622, 4689, 5737
GsaI CCCAGC 3 cut(s) 466, 3822, 5095
GsuI CTGGAG 6 cut(s) 33, 224, 890, 1733, 2476, 2517
HaeII RGCGCY 3 cut(s) 1217, 2289, 4761
HapII CCGG 3 cut(s) 1787, 3342, 6009
HgaI GACGC 2 cut(s) 1043, 5346
HhaI GCGC 4 cut(s) 566, 1216, 2288, 4760
Hin1I GRCGYC 1 cut(s) 1035
Hin6I GCGC 4 cut(s) 564, 1214, 2286, 4758
HinP1I GCGC 4 cut(s) 564, 1214, 2286, 4758
HincII GTYRAC 4 cut(s) 1853, 2085, 2968, 5497
HindII GTYRAC 4 cut(s) 1853, 2085, 2968, 5497
HindIII AAGCTT 4 cut(s) 940, 1729, 4774, 5805
HpaI GTTAAC 2 cut(s) 2085, 2968
HpaII CCGG 3 cut(s) 1787, 3342, 6009
HpyCH4IV ACGT 8 cut(s) 340, 443, 1289, 4041, 4508, 5499, 5569, 6112
HpySE526I ACGT 8 cut(s) 340, 443, 1289, 4041, 4508, 5499, 5569, 6112
Hsp92I GRCGYC 1 cut(s) 1035
HspAI GCGC 4 cut(s) 564, 1214, 2286, 4758
Kpn2I TCCGGA 2 cut(s) 1786, 6008
Ksp22I TGATCA 1 cut(s) 1183
KspAI GTTAAC 2 cut(s) 2085, 2968
LguI GCTCTTC 2 cut(s) 3677, 5928
LmnI GCTCC 9 cut(s) 14, 561, 870, 996, 1396, 1553, 2495, 2893, 4765
Lsp1109I GCAGC 8 cut(s) 315, 1114, 2528, 2726, 2879, 3159, 4700, 5723
MabI ACCWGGT 1 cut(s) 6064
MaeII ACGT 8 cut(s) 340, 443, 1289, 4041, 4508, 5499, 5569, 6112
MbiI CCGCTC 2 cut(s) 69, 991
MfeI CAATTG 2 cut(s) 608, 5283
MflI RGATCY 5 cut(s) 366, 2844, 4074, 5467, 5542
MlsI TGGCCA 1 cut(s) 3376
MluNI TGGCCA 1 cut(s) 3376
MlyI GAGTC 8 cut(s) 500, 1318, 2255, 2454, 2868, 4532, 5422, 5907
MmeI TCCRAC 6 cut(s) 139, 530, 911, 1440, 3674, 5691
Mox20I TGGCCA 1 cut(s) 3376
Mph1103I ATGCAT 6 cut(s) 1141, 1515, 2236, 2748, 3067, 4991
MroI TCCGGA 2 cut(s) 1786, 6008
MroXI GAANNNNTTC 2 cut(s) 3101, 5275
MscI TGGCCA 1 cut(s) 3376
MslI CAYNNNNRTG 7 cut(s) 620, 999, 3037, 3226, 4463, 5486, 5772
Msp20I TGGCCA 1 cut(s) 3376
MspA1I CMGCKG 2 cut(s) 2717, 4826
MspCI CTTAAG 2 cut(s) 3385, 3703
MspI CCGG 3 cut(s) 1787, 3342, 6009
MunI CAATTG 2 cut(s) 608, 5283
Mva1269I GAATGC 4 cut(s) 1141, 2165, 3646, 5783
MvnI CGCG 2 cut(s) 2387, 3330
NcoI CCATGG 1 cut(s) 191
NdeI CATATG 2 cut(s) 3810, 5246
NmeAIII GCCGAG 1 cut(s) 2262
NmuCI GTSAC 6 cut(s) 336, 1365, 2257, 2819, 3583, 4504
NsiI ATGCAT 6 cut(s) 1141, 1515, 2236, 2748, 3067, 4991
NspI RCATGY 9 cut(s) 337, 688, 2750, 4666, 5389, 5528, 5555, 5690, 5913
OliI CACNNNNGTG 3 cut(s) 620, 3037, 5772
PaeI GCATGC 2 cut(s) 4666, 5528
PagI TCATGA 2 cut(s) 2886, 5026
PceI AGGCCT 2 cut(s) 3701, 5721
PciI ACATGT 3 cut(s) 333, 684, 5686
PciSI GCTCTTC 2 cut(s) 3677, 5928
PctI GAATGC 4 cut(s) 1141, 2165, 3646, 5783
PdmI GAANNNNTTC 2 cut(s) 3101, 5275
PflMI CCANNNNNTGG 1 cut(s) 2457
PfoI TCCNGGA 2 cut(s) 1351, 5470
PkrI GCNGC 9 cut(s) 305, 1129, 2518, 2716, 2869, 3149, 3623, 4690, 5738
PleI GAGTC 8 cut(s) 499, 1317, 2254, 2453, 2868, 4532, 5421, 5906
PpsI GAGTC 8 cut(s) 499, 1317, 2254, 2453, 2868, 4532, 5421, 5906
Ppu21I YACGTR 2 cut(s) 444, 5570
PpuMI RGGWCCY 1 cut(s) 6137
PscI ACATGT 3 cut(s) 333, 684, 5686
PshBI ATTAAT 3 cut(s) 1835, 1908, 2303
PsiI TTATAA 3 cut(s) 695, 1584, 3713
Psp124BI GAGCTC 1 cut(s) 3672
Psp1406I AACGTT 1 cut(s) 4041
Psp5II RGGWCCY 1 cut(s) 6137
PspFI CCCAGC 3 cut(s) 462, 3818, 5091
PspOMI GGGCCC 2 cut(s) 1447, 4974
PspPPI RGGWCCY 1 cut(s) 6137
PsrI GAACNNNNNNTAC 4 cut(s) 693, 725, 1479, 1511
PstI CTGCAG 5 cut(s) 2560, 4485, 4498, 5185, 5412
PstNI CAGNNNCTG 1 cut(s) 2105
PsuI RGATCY 5 cut(s) 366, 2844, 4074, 5467, 5542
PvuII CAGCTG 1 cut(s) 2717
RsaI GTAC 9 cut(s) 614, 1024, 1228, 1386, 1668, 2649, 3762, 5502, 6194
RsaNI GTAC 9 cut(s) 613, 1023, 1227, 1385, 1667, 2648, 3761, 5501, 6193
RseI CAYNNNNRTG 7 cut(s) 620, 999, 3037, 3226, 4463, 5486, 5772
SacI GAGCTC 1 cut(s) 3672
SapI GCTCTTC 2 cut(s) 3677, 5928
SatI GCNGC 9 cut(s) 304, 1128, 2517, 2715, 2868, 3148, 3622, 4689, 5737
ScaI AGTACT 1 cut(s) 1024
SchI GAGTC 8 cut(s) 500, 1318, 2255, 2454, 2868, 4532, 5422, 5907
SexAI ACCWGGT 1 cut(s) 6064
SfcI CTRYAG 8 cut(s) 1846, 2106, 2556, 4481, 4494, 4964, 5181, 5408
SinI GGWCC 6 cut(s) 71, 188, 2707, 4828, 5963, 6137
SmiMI CAYNNNNRTG 7 cut(s) 620, 999, 3037, 3226, 4463, 5486, 5772
SmlI CTYRAG 7 cut(s) 797, 869, 1409, 3385, 3457, 3703, 3950
SmoI CTYRAG 7 cut(s) 797, 869, 1409, 3385, 3457, 3703, 3950
SnaBI TACGTA 2 cut(s) 444, 5570
SpeI ACTAGT 1 cut(s) 1340
SphI GCATGC 2 cut(s) 4666, 5528
SseBI AGGCCT 2 cut(s) 3701, 5721
SspI AATATT 5 cut(s) 1588, 2804, 2931, 3058, 5099
SstI GAGCTC 1 cut(s) 3672
StuI AGGCCT 2 cut(s) 3701, 5721
StyI CCWWGG 3 cut(s) 191, 718, 4978
TaiI ACGT 8 cut(s) 343, 446, 1292, 4044, 4511, 5502, 5572, 6115
TaqI TCGA 4 cut(s) 2479, 3603, 3745, 4212
TaqII GACCGA 1 cut(s) 1492
TatI WGTACW 4 cut(s) 612, 1022, 1384, 2647
TauI GCSGC 1 cut(s) 3624
TseFI GTSAC 6 cut(s) 336, 1365, 2257, 2819, 3583, 4504
TseI GCWGC 8 cut(s) 303, 1127, 2516, 2714, 2867, 3147, 4688, 5736
Tsp45I GTSAC 6 cut(s) 336, 1365, 2257, 2819, 3583, 4504
TspGWI ACGGA 1 cut(s) 541
Van91I CCANNNNNTGG 1 cut(s) 2457
Vha464I CTTAAG 2 cut(s) 3385, 3703
VneI GTGCAC 3 cut(s) 2004, 5227, 5682
VpaK11BI GGWCC 6 cut(s) 71, 188, 2707, 4828, 5963, 6137
VspI ATTAAT 3 cut(s) 1835, 1908, 2303
XceI RCATGY 9 cut(s) 337, 688, 2750, 4666, 5389, 5528, 5555, 5690, 5913
XcmI CCANNNNNNNNNTGG 1 cut(s) 3033
XmaJI CCTAGG 1 cut(s) 4978
XmiI GTMKAC 1 cut(s) 5572
XmnI GAANNNNTTC 2 cut(s) 3101, 5275
ZrmI AGTACT 1 cut(s) 1024
Zsp2I ATGCAT 6 cut(s) 1141, 1515, 2236, 2748, 3067, 4991
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.