FvH4_2g02080

Urb2/Npa2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000376
Physical Location & Seq
Reverse (-)
1735486 .. 1745970
10485 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02080.t2

Sequence Viewer

Length: 5946 bp
ATGTCGAGAGTAAAGGAAGAAGGACGGAGCAGTGATGAGGATGATCAAGCAGTGAACATGTCACGTTTAGTAATGTTTGGCAGTGAGTGGATCCAGTCGTTGTTGATTTCTGAAGGGAAGAAGGTTCAGAGTGGTGGAGACAAGCATCAAGCTGAAGCTATTGATACGTTTTTGGATTTAAGATGCTGGGAGATTTTTAAATTCTGTCTGGAGGAGTCTCTGAAATTAAATGTTTCCTTAAGTTTCTCACGGAACCTTTTACGCTCTATATGTTGGATTGCAAGGAGTGCACTGTCCTTAATGAACCCAACATCTTCATCTCCAAGAGATTTTTTTGCCATTGGTGAAGGGTTACAGTTGTACACTACTGTGCTTGATTGTATTTCTATGGTATTTTCATCCCAAGAAGGCTTCTCAAATGAAAATTTGGACATGTGGATTTCAACTGTAAGTTCAGTTCTTGACCTTGGACACAAATTTTACAGTGAAGCTCCTGTTAGTCATGATGAGGGTGTATTTGTTTCCCGATTCTTGTGCTTGGTGCTTGAGCCATTTGCCAGGTTTTTAAGGGCTCACGGAGCTCGGAAAAATGGATTTCATGATTTTAGTGAGAAGCTTCTTGAGCCTCTCTTGCATCTCTACGGTCTCTTGCCTCTTCAGATTGGTGGAAGTAATCCTGGTTGGACGAGAAACTTACTGAAGCTTGTTGAAGATGTATTATCTCATGGGCTATATCACCCACTCCATATTGATGGGTTTTTGAGCTTATGCAGTACTGAAAAATACGCCACATCCAAATATGAGAAGTCAAAAGATTCCAAAACAATGAACGAGAGCTATCATAGACATTTATTTGATAAAGTGGAAGGAATTTTAGCTGCAAAGAATGCATTTGCAGTAGAAAGCATAGGAGTCTTGTTTCAACTGCTTATAGATCAAGTTAAAAAGCTAAAGACGGATTCAGCGCTGACTGCAAGTGGCAAAATGATGGGGAAAAGTGAAGGTTCAAGGCATATAGAAGATAGTTCATTGGGTCAAACATCTATGATGTCTTCTGAGAGTCGTATTATTGAAACAAACTATCACTCAACTAGTTTCAATCTGGAAACACGAAGGTCCCTTTACTATTTTTTTGTACTGATTATGGAGCCTCATTTGCTTGAGATCAATGGCTTTCTTGAAACTAAGCTGAAAGTGGGCCCTGAATTGTTGGATGTTCGTTGCACACTTAAAGCCATCAATAGTGTACTTTTGGTCTTTATGCATGAAAAGATTTATCTGAGAACAGAGGATGCCTCTGAGGGAGCTTGTCTTAAATTTTTGAAGAAGGCTTATAATATCGTCATGTCATTGTCCTCTGATTTGATTCAGTTGTCAGTTACACACTCAGATAGTTTTACTCTAATAGCAAATGAGGTACTTTCTGCTGTAGGCTATCTTTTGGAGATTGAATATGATGTTCTAGAGAATGATCTGCTAAGCTTGTGGTTAATGATGCTCTCATACTTGGCCATTGGGTTTTCTTTGGTGGATTCTCCGGATAGATGCTCATTATTCTCCAAGATAATAGATATTGGATGCCAATTAATTATGCTCTATAGTCAACTTCGTCAGGTGGATACTGCTATCTTTGCATTGTGTAAAGCAATGAGGGTTATTAATTTACATAATATCGATGGTGATCTGAACTATGGTAGATTTGTCATTCCTTTTCATGGTGAAGCATATGCAAAATCTGTGGAAATGATATCATGTGCTCACCACCTCAAAATTGCAATCCACAAAGCAATGAAGTCCATACCAGAAGGACAAGCCAGTCAGTGTATTCAGCAGTTAACATTGGATATATTAGAATCTTTAGAGTGGATGAAAGCTAGTTGTTTGGAGGCTGATGAAAATGAATTTGCTGATTGCCATCTAAGTAGCTTGCACAGTTATAATCTGGAAGCTGAACTTTTCGGGAGAGGGTTGTCAGAAATGTATACACTAGTGCTAGAGTCATTGATTGTCACAGCTGGAAACTCTAACCTTCTTAGCGCTTCTATAAAGGAACTGATTAGAGTGATTTCCCCTTGCATGAGCAAGCTGGTTGGACCACAGCAGGAGGATGCTATAAACAAATTCCTATGTTCTGTCGTGGTGAAAGATTCTGACAATGTGGTCGCTAGAAACAAAAAGAAATATCTGATATTTGGAGTCTCCACTTATTGGGTAGTTCTATTCTTCTTTCGACTGTATAGTTCCTGCCGATGCTTATATAGGCAAGCATCAATCCTTATGCCTCCAGATTTATCAAGAAAAATGTCTGCAGAAATGGGAGATTTGTTCTCCTCATTCTCTGGCAGGGATTGGATGGAAATGTCTGATTGGATGAATGAGGGATTTTTCTCTTGGATTGTACAACCTTCAGCTTCTCTTCTTGTTGTTATTCGATCTATTTCAAGCATCTTTTGTAAGGACAGTGCAGCTTATTCTTGTCCTTTAACTTATGTAATGCATGCTATGGCTTGTGAAAGGCTTGTGGATTTGAATAGTCATATAAAGTCTTTTGAATATTTGGTTGAAAATGGTGATAATCTGGCGCAACTTGCGGAGATCTCTTCTTTGAGGCAAGAGGCAGCAGGACTTACTGGTTTTATGATGGGGCACCTCTCATTAGTGTCTGAAGATCAACAGCGAATCTTTACTTCCGCTGATACGACTAATAATAAGATGGTTCTCTATGAAAGTGATGAATGGGATTTCAGTATTTGCTCTGTGAACAAGAAATCATTGCCAACTGCAGTTTGGTGGGTTGTTTGCCAAAATATTCATGCATGGTGTCCTCATGCCTCTGAGAAGGATTTGAAGAGATTCCTTTCTGTTTTAATCCATACTTCCCTTCCCTATGTAAGAAACAATACTGGGGAGGTTATAGAGCTTAAGAACCATGAAGCTGACAGGCTGAAGAATGTGGCATTGCATCAAATCTCATCACACTGCTTTATTGATTCCAGTCTGTATGAGCAAAGATTTGTCCGCAGGTATTTTGCAAAGCTGTTCTGCCGTGCACTGGAGAAATCTACATTACCATTCATCAGTGACTTCCCATCCAGAAATGTTAAATTCAAATCATCACCTAATTGGCCGGACGTCTTAAGTGATTTAGAGAACTCATCACTGGCTATTTCCTGCAACAAACTCAAAGTATTTGATTGTTCCTCAGCCTCAAGTTGCAAGGGAGAAAACTCCCAACCATCTAACATGATGAAATTTACAGCTTGTCAGAGTTTGCTTAATCTTCTGAGTTGTATGCCAAAAGGTCATTTGAACACGAGATCATTCTCACGTTATGTGACATCTATTCTCAACCTTGAAAGGATTGCTGTTGGCGGCTTATTAGATTATCAGAATGCTTCATACTCAACTTATTATTATGAGCTCTTCAGATTGTTTGTGTCTTGTCGGAAGGCCTTGAGATGTGTAATTATTGCTTGTGAGGAGACAATAGCCAGTCAGACCTCAGACACTCGAGTGCTCTTTGAGGATTTATTTCCTGTTTTATGGCTTTACAAGTCAGTGCACATGGTTGCTGGGCTTCAAGAGTCATTCTCAAAAGATATTTATCATCACGTTCATGATATGATCTTGGCGTTAATGGATCACACGTTTTATGTGTTTCTGACATTAACTAAATATGAAACTAATCATGCTATTCGTTTCCTTGAGGTTGCTGAGCTGAATTCTGAATGTTCCCGTGAACAGAGAAGTCCATATTCGTCCAATAATATTAAATCCTGGAAAAGTGTGAACATTGCTGCTAAGATTTTGAAGGAACAGATGCAGATCTTACTTGTAAATGTGAAGGGTGGCATTTGTAAAGAGGGAGTTGCTGTTGATGCTCTAAATTTGAACAAATTTGCTTCCATAATTTCCTGTTTTAGTGGGTTTTTATGGGGACTTGCATGCAGTGTGATCGACACAGATGGGAAAAATAGTGATGAGAAGGCTAAGTTGTCAAGGTGGAAACCTGAACCAGTCTCCGAACTCGACCTCTGTATAAACGCCTTTGCAGAAATTTCTAGTCTTCTATTACAAATGTTTATTCTTGACGATAATCAGCAGCCCACAACTATATGTGACACTTATAATCCTCAGAAGTCAGGCTACAATTTAGATTTGTTGGGTGCAGAGAAGATTTCGCCAGAAGACAATAATTCGGTCACTGATATGGCATGTGGTGGACTGCAGGATGAATCTGCAGTTGCAGTCGCATGCTCAGCATCATCCGACATCTGTGATGACTCTGTAATTGGTAGTGTTCATAGAAGAAGACCGCGCTTGAAAGATGCAAATAGTGTTGTTAGTGTTCTCAGTGCTGTTGATTCATTTGAACTGCAATCTCTAAATAAGCCTCTGTTGAAAAGCATGCTAAAAGGTGATTTCCCCAATGCAGCATTTTTACTCAGGCAGTTGTTGATTGCCTCTTCTGCTGTTTTGAGGCTAAATTTGCATATTAAGAGTGCTCCCATGTCGTCAAGCTTGGTGCATAAGTTTGCTGGCATTATGCAAGTGGTGTTATTGGAATCAGTGGACGCAAGTCAGGTTCCACACTTCTATTATTTTGTGTGTTTAGATGGTGTTCTGAAGTATCTGGAAGAATTAGGGAATCATTTTCCTTTGACTAAGCCTACCTTATCCAAAGATCTGTTTGCCAAGATGGTTCAGCTACAGTTATGGGCTCTAGGGAAATGCATAACTTTACAAGGGAAAAGAGCAACTCTTGCATCTCATGAAACAAGCACAAATACACACCTTTCTCCTATGGGATTTTCTGAAGCATCAACTCTTTCTGGCTGTGAATACTTACTGGATGATTGCAAAGCTAGGTTGAGGTCATCATTCGCAGTGTTCATAAAGAAATCAACAGAGTTGCATCTACAATCTGCAGTTAAAGCTATCGAAAGAGCTTTAGTTGGTGTGCAGGAGGGGTGCACAGTGAGGTATGACATATGTGCTGTTAGTGACGATGGAGGAAATGTATCTTATATTGTTGCAGCTGGCATTGATTGCTTGGATTTGGTTCTGGAATTTGTTTCAGGACACAATTTGAGTGTGGTTAAAAAATGCATTCAGAGGTTGATTGCCTGCATGTTCAATGTTATTCTGCATCTGCAGAGTCCACTAATATTCTATGAGAGATCCACTCCGAGTAAAGAGCCAGATCCAGGAACAGTCATTCTTATGTGTGTTGATGTTCTGGCAAGAATTTCAGGCAAGCATGCAATATACAAAATGAATCTCTGGCATGTAGCACACTCGTTACGTATACCTTCAGCACTTTTTCAAGATTTCCATCTCTTGAAACAATCCAAATGTCGTGTTCCAAATGATTCCTCTACAAGTACAAATAACCAACTCTGTAACCCAGCAGCAAGTATACACGTCTCTGGTGTAGATAGGCAATACTCAACCGGCCTATATTCTGCTTGCTGCCGATTATTGCATAATGTTGTGAAGCATCACAAAAGTGAATGCGAGGGGTATGTTGCTTTACTTCAAGCTTCTGTTCATGTTCTTCTTTATTGTTTGGAGACATTGGATGCTGTTGTGGTAGCCAAGGAAGGCTTGTTTTCATGGGAAGTAGAAGAAGGAGTCAAATGTGCATGTTCACTCAGAAGGATTTATGAAGAGCTAAGACAACAAAAAGAGGTCTTTGGTCCACACTGTTACCACTTCTTAGCTTATTACATATGGGTTTATTCAGGATATGGCCCCCGCAAAACAGGCATCAAAAGGGAAATAGATGAAGCTCTAAGACCAGGTGTATATGCTCTCATAGATGTTTGCTCACCAGATGATCTTCAACGTCTTCATACCTCATTTGGAGAGGGTCCTTGCAGAAACACTTTAGCAACCTTGAAGCACGATTACGAACTCAATTTCCAGTACCAGGGAAAAGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Urb2

Protein Analysis

1982

Amino Acids

222.42

Weight (kDa)

6.17

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Urb2 PF10441 1838 - 2076 9.2e-39 Urb2/Npa2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30150 AT4G30150
fragaria_vesca FvH4_2g02080 FvH4_2g02080
malus_domestica MD05G1115300.v1.1 MD10G1117200.v1.1 MD10G1117300.v1.1 MD10G1117400.v1.1 MD10G1117500.v1.1 MD10G1117600.v1.1 MD10G1117700.v1.1
prunus_persica Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1 Prupe.8G159900_v2.0.a1
pyrus_communis pycom05g10980 pycom10g10170
rosa_chinensis RchiOBHm_Chr2g0118221 RchiOBHm_Chr6g0244891 RchiOBHm_Chr6g0244901 RchiOBHm_Chr6g0244911 RchiOBHm_Chr6g0244921 RchiOBHm_Chr6g0244931 RchiOBHm_Chr6g0244961 RchiOBHm_Chr6g0244971 RchiOBHm_Chr6g0245301 RchiOBHm_Chr6g0245311 RchiOBHm_Chr6g0245351 RchiOBHm_Chr6g0245361 RchiOBHm_Chr6g0245441 RchiOBHm_Chr6g0245521 RchiOBHm_Chr6g0245531 RchiOBHm_Chr6g0245571
rosa_laevigata RLG00000015303
rosa_multiflora Rmu_sc0000471.1_g000006 Rmu_sc0000471.1_g000007 Rmu_sc0000471.1_g000008 Rmu_sc0001288.1_g000012 Rmu_sc0001925.1_g000002 Rmu_sc0002202.1_g000009 Rmu_sc0002202.1_g000016 Rmu_sc0002202.1_g000017 Rmu_sc0002202.1_g000019 Rmu_sc0002202.1_g000020 Rmu_ssc0000087.1_g000055 Rmu_ssc0000087.1_g000056
rosa_roxburghii Rroxscaffold_3G00242140 Rroxscaffold_7G00215270
rosa_rugosa Rorug05G0511800 Rorug05G0513500 Rorug05G0513900 Rorug05G0514000 Rorug05G0514200 Rorug05G0514400
rosa_samantha Rh1AG142300 Rh1DG147200 Rh6AG026300 Rh6AG026400 Rh6AG026500 Rh6AG028900 Rh6AG029000 Rh6AG029100 Rh6AG029200 Rh6AG029300 Rh6AG029400 Rh6AG029500 Rh6BG025200 Rh6CG020200 Rh6CG020300 Rh6CG020400 Rh6CG023100 Rh6CG023300 Rh6CG023500 Rh6CG023800 Rh6CG024100 Rh6CG024200 Rh6CG024400 Rh6CG024700 Rh6CG024800 Rh6CG025200 Rh6CG025400 Rh6CG025600 Rh6CG025700 Rh6CG025800 Rh6CG025900 Rh6CG026000 Rh6DG020800 Rh6DG020900 Rh6DG021000 Rh6DG023100 Rh6DG023300 Rh6DG023500 Rh6DG023700 Rh6DG023900 Rh6DG024200 Rh6DG024500 Rh6DG024700 Rh6DG024900 Rh6DG025000 Rh6DG025200 Rh6DG025300 Rh6DG025400
rosa_wichuraiana Rw2G021630 Rw2G021750 Rw6G002430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1335, 1938, 4128
AasI GACNNNNNNGTC 2 cut(s) 1815, 2159
AatII GACGTC 1 cut(s) 3135
Acc36I ACCTGC 1 cut(s) 3012
AccB1I GGYRCC 1 cut(s) 2646
AccB7I CCANNNNNTGG 1 cut(s) 2208
AccI GTMKAC 3 cut(s) 1982, 5308, 5419
AccII CGCG 1 cut(s) 4318
AccIII TCCGGA 1 cut(s) 1537
AciI CCGC 6 cut(s) 2591, 2691, 3019, 3372, 4316, 5758
AclWI GGATC 5 cut(s) 85, 98, 3648, 5175, 5198
AcoI YGGCCR 2 cut(s) 1509, 3125
AcyI GRCGYC 1 cut(s) 3132
AfaI GTAC 8 cut(s) 362, 775, 1137, 1248, 1419, 2400, 5386, 5930
AfeI AGCGCT 2 cut(s) 966, 2038
AfiI CCNNNNNNNGG 5 cut(s) 1715, 2208, 3052, 5207, 5932
AflII CTTAAG 3 cut(s) 238, 2921, 3136
AflIII ACRYGT 4 cut(s) 57, 432, 3645, 5422
AhlI ACTAGT 2 cut(s) 1091, 1987
AjiI CACGTC 1 cut(s) 5425
AjnI CCWGG 6 cut(s) 557, 676, 3776, 5206, 5800, 5931
AleI CACNNNNGTG 3 cut(s) 368, 3512, 5508
AloI GAACNNNNNNTCC 2 cut(s) 5907, 5939
Alw21I GWGCWC 9 cut(s) 292, 583, 1759, 3052, 3423, 3519, 3564, 4507, 4976
Alw26I GTCTC 8 cut(s) 132, 222, 650, 2203, 3476, 4024, 5431, 5567
Alw44I GTGCAC 4 cut(s) 288, 3048, 3560, 4972
AlwI GGATC 5 cut(s) 85, 98, 3648, 5175, 5198
Ama87I CYCGRG 1 cut(s) 3510
Aor13HI TCCGGA 1 cut(s) 1537
Aor51HI AGCGCT 2 cut(s) 966, 2038
AoxI GGCC 6 cut(s) 1198, 1509, 3125, 3450, 5455, 5752
ApaI GGGCCC 1 cut(s) 1202
ApaLI GTGCAC 4 cut(s) 288, 3048, 3560, 4972
ApeKI GCWGC 9 cut(s) 878, 2465, 2618, 3797, 4102, 4433, 5036, 5411, 5472
AseI ATTAAT 2 cut(s) 1586, 1659
Asp700I GAANNNNTTC 1 cut(s) 2852
AspLEI GCGC 4 cut(s) 967, 2039, 2584, 4320
AspS9I GGNCC 7 cut(s) 1116, 1198, 1199, 2093, 5699, 5753, 5873
AvaI CYCGRG 1 cut(s) 3510
AvaII GGWCC 4 cut(s) 1116, 2093, 5699, 5873
BaeGI GKGCMC 6 cut(s) 292, 1202, 2649, 3052, 3564, 4976
BalI TGGCCA 1 cut(s) 1511
BamHI GGATCC 1 cut(s) 90
BanI GGYRCC 1 cut(s) 2646
BanII GRGCYC 5 cut(s) 574, 583, 1202, 3423, 4723
BarI GAAGNNNNNNTAC 4 cut(s) 4130, 4162, 5894, 5926
BauI CACGAG 1 cut(s) 3313
BbsI GAAGAC 5 cut(s) 1044, 4058, 4194, 4318, 5843
Bbv12I GWGCWC 9 cut(s) 292, 583, 1759, 3052, 3423, 3519, 3564, 4507, 4976
BbvCI CCTCAGC 1 cut(s) 3202
BbvI GCAGC 9 cut(s) 865, 2477, 2630, 3784, 4114, 4445, 5048, 5423, 5459
BceAI ACGGC 1 cut(s) 3030
BcgI CGANNNNNNTGC 4 cut(s) 516, 550, 3937, 3971
BciT130I CCWGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
BciVI GTATCC 1 cut(s) 1612
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 8 cut(s) 132, 222, 650, 2203, 3476, 4024, 5431, 5567
BcuI ACTAGT 2 cut(s) 1091, 1987
BfaI CTAG 9 cut(s) 1092, 1463, 1875, 1988, 1994, 2166, 4062, 4724, 4866
BfmI CTRYAG 9 cut(s) 1428, 1597, 2307, 2781, 4226, 4239, 4709, 4926, 5153
BfoI RGCGCY 2 cut(s) 968, 2040
BfrI CTTAAG 3 cut(s) 238, 2921, 3136
BfuAI ACCTGC 1 cut(s) 3012
BfuI GTATCC 1 cut(s) 1612
BglII AGATCT 3 cut(s) 2595, 3825, 4684
BlpI GCTNAGC 3 cut(s) 1478, 3714, 4258
BmcAI AGTACT 1 cut(s) 775
Bme1390I CCNGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
Bme18I GGWCC 4 cut(s) 1116, 2093, 5699, 5873
BmeT110I CYCGRG 1 cut(s) 3510
BmgBI CACGTC 1 cut(s) 5425
BmgT120I GGNCC 7 cut(s) 1116, 1198, 1199, 2093, 5699, 5753, 5873
BmiI GGNNCC 9 cut(s) 92, 254, 1118, 1149, 1200, 2648, 4587, 5755, 5874
BmrFI CCNGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
BmrI ACTGGG 1 cut(s) 2913
BmuI ACTGGG 1 cut(s) 2913
BoxI GACNNNNGTC 1 cut(s) 4578
BpiI GAAGAC 5 cut(s) 1044, 4058, 4194, 4318, 5843
BplI GAGNNNNNCTC 6 cut(s) 1280, 1312, 3575, 3607, 5170, 5202
BpmI CTGGAG 3 cut(s) 230, 2268, 3074
Bpu10I CCTNAGC 1 cut(s) 3202
Bpu1102I GCTNAGC 3 cut(s) 1478, 3714, 4258
BpuEI CTTGAG 6 cut(s) 566, 641, 1181, 3193, 3475, 3725
Bsa29I ATCGAT 1 cut(s) 1674
BsaAI YACGTR 1 cut(s) 5306
BsaBI GATNNNNATC 5 cut(s) 1680, 1914, 3603, 3824, 5334
BsaHI GRCGYC 1 cut(s) 3132
BsaI GGTCTC 1 cut(s) 650
BsaJI CCNNGG 3 cut(s) 466, 5598, 5932
BsaWI WCCGGW 1 cut(s) 1537
BsaXI ACNNNNNCTCC 4 cut(s) 3858, 3888, 5004, 5034
Bsc4I CCNNNNNNNGG 5 cut(s) 1715, 2208, 3052, 5207, 5932
Bse118I RCCGGY 1 cut(s) 5453
Bse3DI GCAATG 5 cut(s) 1653, 1794, 2771, 2957, 3792
Bse8I GATNNNNATC 5 cut(s) 1680, 1914, 3603, 3824, 5334
BseAI TCCGGA 1 cut(s) 1537
BseBI CCWGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
BseCI ATCGAT 1 cut(s) 1674
BseDI CCNNGG 3 cut(s) 466, 5598, 5932
BseJI GATNNNNATC 5 cut(s) 1680, 1914, 3603, 3824, 5334
BseLI CCNNNNNNNGG 5 cut(s) 1715, 2208, 3052, 5207, 5932
BseMI GCAATG 5 cut(s) 1653, 1794, 2771, 2957, 3792
BseRI GAGGAG 3 cut(s) 227, 2320, 3494
BseSI GKGCMC 6 cut(s) 292, 1202, 2649, 3052, 3564, 4976
BseXI GCAGC 9 cut(s) 865, 2477, 2630, 3784, 4114, 4445, 5048, 5423, 5459
BseYI CCCAGC 3 cut(s) 186, 3572, 5407
BsgI GTGCAG 3 cut(s) 2484, 4188, 4982
Bsh1236I CGCG 1 cut(s) 4318
BshFI GGCC 6 cut(s) 1200, 1511, 3127, 3452, 5457, 5754
BshNI GGYRCC 1 cut(s) 2646
BshVI ATCGAT 1 cut(s) 1674
BsiHKAI GWGCWC 9 cut(s) 292, 583, 1759, 3052, 3423, 3519, 3564, 4507, 4976
BsiHKCI CYCGRG 1 cut(s) 3510
BsiSI CCGG 3 cut(s) 1538, 3128, 5454
BslFI GGGAC 2 cut(s) 1102, 3951
BslI CCNNNNNNNGG 5 cut(s) 1715, 2208, 3052, 5207, 5932
BsmAI GTCTC 8 cut(s) 132, 222, 650, 2203, 3476, 4024, 5431, 5567
BsmBI CGTCTC 1 cut(s) 5431
BsmFI GGGAC 2 cut(s) 1102, 3951
BsmI GAATGC 4 cut(s) 892, 3397, 5109, 5519
BsnI GGCC 6 cut(s) 1200, 1511, 3127, 3452, 5457, 5754
Bso31I GGTCTC 1 cut(s) 650
BsoBI CYCGRG 1 cut(s) 3510
Bsp120I GGGCCC 1 cut(s) 1198
Bsp13I TCCGGA 1 cut(s) 1537
Bsp1407I TGTACA 2 cut(s) 360, 2398
Bsp1720I GCTNAGC 3 cut(s) 1478, 3714, 4258
BspACI CCGC 6 cut(s) 2591, 2691, 3019, 3372, 4316, 5758
BspANI GGCC 6 cut(s) 1200, 1511, 3127, 3452, 5457, 5754
BspDI ATCGAT 1 cut(s) 1674
BspEI TCCGGA 1 cut(s) 1537
BspFNI CGCG 1 cut(s) 4318
BspHI TCATGA 4 cut(s) 502, 598, 3616, 4771
BspLI GGNNCC 9 cut(s) 92, 254, 1118, 1149, 1200, 2648, 4587, 5755, 5874
BspMAI CTGCAG 6 cut(s) 2311, 2785, 4230, 4243, 4930, 5157
BspMI ACCTGC 1 cut(s) 3012
BspPI GGATC 5 cut(s) 85, 98, 3648, 5175, 5198
BspQI GCTCTTC 2 cut(s) 3428, 5664
BspT107I GGYRCC 1 cut(s) 2646
BspTI CTTAAG 3 cut(s) 238, 2921, 3136
BspTNI GGTCTC 1 cut(s) 650
BsrDI GCAATG 5 cut(s) 1653, 1794, 2771, 2957, 3792
BsrFI RCCGGY 1 cut(s) 5453
BsrGI TGTACA 2 cut(s) 360, 2398
BssAI RCCGGY 1 cut(s) 5453
BssECI CCNNGG 3 cut(s) 466, 5598, 5932
BssNAI GTATAC 3 cut(s) 1983, 5309, 5420
BssNI GRCGYC 1 cut(s) 3132
BssSI CACGAG 1 cut(s) 3313
BssT1I CCWWGG 2 cut(s) 466, 5598
Bst1107I GTATAC 3 cut(s) 1983, 5309, 5420
Bst2BI CACGAG 1 cut(s) 3313
Bst2UI CCWGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
Bst6I CTCTTC 7 cut(s) 660, 2421, 2605, 2843, 3428, 4471, 5664
BstACI GRCGYC 1 cut(s) 3132
BstAFI CTTAAG 3 cut(s) 238, 2921, 3136
BstAPI GCANNNNNTGC 4 cut(s) 287, 887, 4763, 5049
BstAUI TGTACA 2 cut(s) 360, 2398
BstBAI YACGTR 1 cut(s) 5306
BstFNI CGCG 1 cut(s) 4318
BstH2I RGCGCY 2 cut(s) 968, 2040
BstHHI GCGC 4 cut(s) 967, 2039, 2584, 4320
BstMAI GTCTC 8 cut(s) 132, 222, 650, 2203, 3476, 4024, 5431, 5567
BstNI CCWGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
BstPAI GACNNNNGTC 1 cut(s) 4578
BstSCI CCNGG 6 cut(s) 557, 676, 3776, 5206, 5800, 5931
BstSFI CTRYAG 9 cut(s) 1428, 1597, 2307, 2781, 4226, 4239, 4709, 4926, 5153
BstSLI GKGCMC 6 cut(s) 292, 1202, 2649, 3052, 3564, 4976
BstSNI TACGTA 1 cut(s) 5306
BstUI CGCG 1 cut(s) 4318
BstV1I GCAGC 9 cut(s) 865, 2477, 2630, 3784, 4114, 4445, 5048, 5423, 5459
BstV2I GAAGAC 5 cut(s) 1044, 4058, 4194, 4318, 5843
BstX2I RGATCY 6 cut(s) 90, 2595, 3825, 4684, 5180, 5203
BstXI CCANNNNNNTGG 1 cut(s) 752
BstYI RGATCY 6 cut(s) 90, 2595, 3825, 4684, 5180, 5203
BstZ17I GTATAC 3 cut(s) 1983, 5309, 5420
Bsu15I ATCGAT 1 cut(s) 1674
BsuI GTATCC 1 cut(s) 1612
BsuRI GGCC 6 cut(s) 1200, 1511, 3127, 3452, 5457, 5754
BsuTUI ATCGAT 1 cut(s) 1674
BtrI CACGTC 1 cut(s) 5425
BtsI GCAGTG 6 cut(s) 37, 57, 88, 2977, 3955, 4893
BveI ACCTGC 1 cut(s) 3012
CciI TCATGA 4 cut(s) 502, 598, 3616, 4771
CfoI GCGC 4 cut(s) 967, 2039, 2584, 4320
Cfr10I RCCGGY 1 cut(s) 5453
Cfr13I GGNCC 7 cut(s) 1116, 1198, 1199, 2093, 5699, 5753, 5873
ClaI ATCGAT 1 cut(s) 1674
CseI GACGC 1 cut(s) 4583
CsiI ACCWGGT 1 cut(s) 5800
Csp6I GTAC 8 cut(s) 361, 774, 1136, 1247, 1418, 2399, 5385, 5929
CspCI CAANNNNNGTGG 2 cut(s) 1719, 1754
CviQI GTAC 8 cut(s) 361, 774, 1136, 1247, 1418, 2399, 5385, 5929
DraI TTTAAA 1 cut(s) 199
DrdI GACNNNNNNGTC 2 cut(s) 1815, 2159
DseDI GACNNNNNNGTC 2 cut(s) 1815, 2159
EaeI YGGCCR 2 cut(s) 1509, 3125
Eam1104I CTCTTC 7 cut(s) 660, 2421, 2605, 2843, 3428, 4471, 5664
EarI CTCTTC 7 cut(s) 660, 2421, 2605, 2843, 3428, 4471, 5664
Ecl136II GAGCTC 2 cut(s) 581, 3421
Eco105I TACGTA 1 cut(s) 5306
Eco130I CCWWGG 2 cut(s) 466, 5598
Eco147I AGGCCT 1 cut(s) 3452
Eco24I GRGCYC 5 cut(s) 574, 583, 1202, 3423, 4723
Eco31I GGTCTC 1 cut(s) 650
Eco32I GATATC 1 cut(s) 1749
Eco47I GGWCC 4 cut(s) 1116, 2093, 5699, 5873
Eco47III AGCGCT 2 cut(s) 966, 2038
Eco53kI GAGCTC 2 cut(s) 581, 3421
Eco88I CYCGRG 1 cut(s) 3510
EcoICRI GAGCTC 2 cut(s) 581, 3421
EcoO109I RGGNCCY 3 cut(s) 1116, 1199, 5873
EcoRI GAATTC 1 cut(s) 3721
EcoRII CCWGG 6 cut(s) 557, 676, 3776, 5206, 5800, 5931
EcoRV GATATC 1 cut(s) 1749
EcoT14I CCWWGG 2 cut(s) 466, 5598
EcoT22I ATGCAT 6 cut(s) 892, 1266, 2499, 2818, 4736, 5111
EcoT38I GRGCYC 5 cut(s) 574, 583, 1202, 3423, 4723
ErhI CCWWGG 2 cut(s) 466, 5598
Esp3I CGTCTC 1 cut(s) 5431
FalI AAGNNNNNCTT 6 cut(s) 1938, 1970, 4658, 4690, 5591, 5623
FaqI GGGAC 2 cut(s) 1102, 3951
FauI CCCGC 1 cut(s) 5765
FauNDI CATATG 3 cut(s) 1726, 4991, 5732
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 3 cut(s) 1982, 5308, 5419
FriOI GRGCYC 5 cut(s) 574, 583, 1202, 3423, 4723
FspBI CTAG 9 cut(s) 1092, 1463, 1875, 1988, 1994, 2166, 4062, 4724, 4866
GlaI GCGC 4 cut(s) 966, 2038, 2583, 4319
GsaI CCCAGC 3 cut(s) 190, 3576, 5411
GsuI CTGGAG 3 cut(s) 230, 2268, 3074
HaeII RGCGCY 2 cut(s) 968, 2040
HaeIII GGCC 6 cut(s) 1200, 1511, 3127, 3452, 5457, 5754
HapII CCGG 3 cut(s) 1538, 3128, 5454
HgaI GACGC 1 cut(s) 4583
HhaI GCGC 4 cut(s) 967, 2039, 2584, 4320
Hin1I GRCGYC 1 cut(s) 3132
Hin6I GCGC 4 cut(s) 965, 2037, 2582, 4318
HinP1I GCGC 4 cut(s) 965, 2037, 2582, 4318
HincII GTYRAC 2 cut(s) 1604, 1836
HindII GTYRAC 2 cut(s) 1604, 1836
HindIII AAGCTT 5 cut(s) 614, 701, 1480, 4519, 5541
HpaI GTTAAC 1 cut(s) 1836
HpaII CCGG 3 cut(s) 1538, 3128, 5454
HpyCH4IV ACGT 9 cut(s) 64, 167, 3132, 3328, 3612, 3647, 5305, 5424, 5848
HpySE526I ACGT 9 cut(s) 64, 167, 3132, 3328, 3612, 3647, 5305, 5424, 5848
Hsp92I GRCGYC 1 cut(s) 3132
HspAI GCGC 4 cut(s) 965, 2037, 2582, 4318
Kpn2I TCCGGA 1 cut(s) 1537
Ksp22I TGATCA 1 cut(s) 43
KspAI GTTAAC 1 cut(s) 1836
LguI GCTCTTC 2 cut(s) 3428, 5664
LmnI GCTCC 6 cut(s) 27, 496, 578, 1147, 1304, 4510
Lsp1109I GCAGC 9 cut(s) 865, 2477, 2630, 3784, 4114, 4445, 5048, 5423, 5459
MabI ACCWGGT 1 cut(s) 5800
MaeI CTAG 9 cut(s) 1092, 1463, 1875, 1988, 1994, 2166, 4062, 4724, 4866
MaeII ACGT 9 cut(s) 64, 167, 3132, 3328, 3612, 3647, 5305, 5424, 5848
MflI RGATCY 6 cut(s) 90, 2595, 3825, 4684, 5180, 5203
MlsI TGGCCA 1 cut(s) 1511
MluNI TGGCCA 1 cut(s) 1511
MlyI GAGTC 9 cut(s) 224, 921, 1069, 2006, 2205, 3593, 4277, 5167, 5643
MmeI TCCRAC 6 cut(s) 254, 662, 1191, 2071, 3425, 4293
Mox20I TGGCCA 1 cut(s) 1511
Mph1103I ATGCAT 6 cut(s) 892, 1266, 2499, 2818, 4736, 5111
MroI TCCGGA 1 cut(s) 1537
MroXI GAANNNNTTC 1 cut(s) 2852
MscI TGGCCA 1 cut(s) 1511
MslI CAYNNNNRTG 7 cut(s) 368, 750, 3512, 3615, 4208, 5222, 5508
Msp20I TGGCCA 1 cut(s) 1511
MspA1I CMGCKG 3 cut(s) 2015, 2693, 5039
MspCI CTTAAG 3 cut(s) 238, 2921, 3136
MspI CCGG 3 cut(s) 1538, 3128, 5454
MspR9I CCNGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
Mva1269I GAATGC 4 cut(s) 892, 3397, 5109, 5519
MvaI CCWGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
MvnI CGCG 1 cut(s) 4318
NdeI CATATG 3 cut(s) 1726, 4991, 5732
NlaIV GGNNCC 9 cut(s) 92, 254, 1118, 1149, 1200, 2648, 4587, 5755, 5874
NmuCI GTSAC 7 cut(s) 60, 2008, 3080, 3334, 4118, 4201, 5003
NsiI ATGCAT 6 cut(s) 892, 1266, 2499, 2818, 4736, 5111
OliI CACNNNNGTG 3 cut(s) 368, 3512, 5508
PaeI GCATGC 5 cut(s) 2501, 3948, 4257, 4411, 5264
PaeR7I CTCGAG 1 cut(s) 3510
PagI TCATGA 4 cut(s) 502, 598, 3616, 4771
PceI AGGCCT 1 cut(s) 3452
PciI ACATGT 2 cut(s) 57, 432
PciSI GCTCTTC 2 cut(s) 3428, 5664
PctI GAATGC 4 cut(s) 892, 3397, 5109, 5519
PdmI GAANNNNTTC 1 cut(s) 2852
PflMI CCANNNNNTGG 1 cut(s) 2208
PfoI TCCNGGA 2 cut(s) 3776, 5206
PleI GAGTC 9 cut(s) 223, 920, 1068, 2005, 2204, 3592, 4277, 5166, 5642
PpsI GAGTC 9 cut(s) 223, 920, 1068, 2005, 2204, 3592, 4277, 5166, 5642
Ppu21I YACGTR 1 cut(s) 5306
PpuMI RGGWCCY 2 cut(s) 1116, 5873
PscI ACATGT 2 cut(s) 57, 432
PshAI GACNNNNGTC 1 cut(s) 4578
PshBI ATTAAT 2 cut(s) 1586, 1659
PsiI TTATAA 3 cut(s) 1335, 1938, 4128
Psp124BI GAGCTC 2 cut(s) 583, 3423
Psp5II RGGWCCY 2 cut(s) 1116, 5873
Psp6I CCWGG 6 cut(s) 557, 676, 3776, 5206, 5800, 5931
PspFI CCCAGC 3 cut(s) 186, 3572, 5407
PspGI CCWGG 6 cut(s) 557, 676, 3776, 5206, 5800, 5931
PspN4I GGNNCC 9 cut(s) 92, 254, 1118, 1149, 1200, 2648, 4587, 5755, 5874
PspOMI GGGCCC 1 cut(s) 1198
PspPI GGNCC 7 cut(s) 1116, 1198, 1199, 2093, 5699, 5753, 5873
PspPPI RGGWCCY 2 cut(s) 1116, 5873
PspXI VCTCGAGB 1 cut(s) 3510
PsrI GAACNNNNNNTAC 2 cut(s) 441, 473
PstI CTGCAG 6 cut(s) 2311, 2785, 4230, 4243, 4930, 5157
PsuI RGATCY 6 cut(s) 90, 2595, 3825, 4684, 5180, 5203
PvuII CAGCTG 2 cut(s) 2015, 5039
RsaI GTAC 8 cut(s) 362, 775, 1137, 1248, 1419, 2400, 5386, 5930
RsaNI GTAC 8 cut(s) 361, 774, 1136, 1247, 1418, 2399, 5385, 5929
RseI CAYNNNNRTG 7 cut(s) 368, 750, 3512, 3615, 4208, 5222, 5508
SacI GAGCTC 2 cut(s) 583, 3423
SapI GCTCTTC 2 cut(s) 3428, 5664
Sau96I GGNCC 7 cut(s) 1116, 1198, 1199, 2093, 5699, 5753, 5873
ScaI AGTACT 1 cut(s) 775
SchI GAGTC 9 cut(s) 224, 921, 1069, 2006, 2205, 3593, 4277, 5167, 5643
ScrFI CCNGG 6 cut(s) 559, 678, 3778, 5208, 5802, 5933
SexAI ACCWGGT 1 cut(s) 5800
SfcI CTRYAG 9 cut(s) 1428, 1597, 2307, 2781, 4226, 4239, 4709, 4926, 5153
Sfr274I CTCGAG 1 cut(s) 3510
SinI GGWCC 4 cut(s) 1116, 2093, 5699, 5873
SlaI CTCGAG 1 cut(s) 3510
SmiMI CAYNNNNRTG 7 cut(s) 368, 750, 3512, 3615, 4208, 5222, 5508
SnaBI TACGTA 1 cut(s) 5306
SpeI ACTAGT 2 cut(s) 1091, 1987
SphI GCATGC 5 cut(s) 2501, 3948, 4257, 4411, 5264
SseBI AGGCCT 1 cut(s) 3452
SsiI CCGC 6 cut(s) 2591, 2691, 3019, 3372, 4316, 5758
SspI AATATT 4 cut(s) 2555, 2809, 3769, 5169
SspMI CTAG 9 cut(s) 1092, 1463, 1875, 1988, 1994, 2166, 4062, 4724, 4866
SstI GAGCTC 2 cut(s) 583, 3423
StuI AGGCCT 1 cut(s) 3452
StyD4I CCNGG 6 cut(s) 557, 676, 3776, 5206, 5800, 5931
StyI CCWWGG 2 cut(s) 466, 5598
TaiI ACGT 9 cut(s) 67, 170, 3135, 3331, 3615, 3650, 5308, 5427, 5851
TaqI TCGA 8 cut(s) 5, 1674, 2230, 2431, 3511, 3957, 4029, 4941
TaqII GACCGA 1 cut(s) 4189
TatI WGTACW 6 cut(s) 360, 773, 1135, 1246, 2398, 5384
TauI GCSGC 1 cut(s) 3375
TseFI GTSAC 7 cut(s) 60, 2008, 3080, 3334, 4118, 4201, 5003
TseI GCWGC 9 cut(s) 878, 2465, 2618, 3797, 4102, 4433, 5036, 5411, 5472
Tsp45I GTSAC 7 cut(s) 60, 2008, 3080, 3334, 4118, 4201, 5003
TspGWI ACGGA 4 cut(s) 40, 265, 591, 971
Van91I CCANNNNNTGG 1 cut(s) 2208
Vha464I CTTAAG 3 cut(s) 238, 2921, 3136
VneI GTGCAC 4 cut(s) 288, 3048, 3560, 4972
VpaK11BI GGWCC 4 cut(s) 1116, 2093, 5699, 5873
VspI ATTAAT 2 cut(s) 1586, 1659
XbaI TCTAGA 1 cut(s) 1462
XcmI CCANNNNNNNNNTGG 1 cut(s) 2784
XhoI CTCGAG 1 cut(s) 3510
XmiI GTMKAC 3 cut(s) 1982, 5308, 5419
XmnI GAANNNNTTC 1 cut(s) 2852
XspI CTAG 9 cut(s) 1092, 1463, 1875, 1988, 1994, 2166, 4062, 4724, 4866
ZraI GACGTC 1 cut(s) 3133
ZrmI AGTACT 1 cut(s) 775
Zsp2I ATGCAT 6 cut(s) 892, 1266, 2499, 2818, 4736, 5111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.